FastQCFastQC Report
Fri 25 Sep 2015
000000000-AJJF6 l01n01.331000000349b6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJJF6 l01n01.331000000349b6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11768177
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCATGGGAGTGGGTTGCACCAGAAGTAGTTAGTCTAACCTTCGGGAG146774512.472152653720284No Hit
ACACCATGGGAGTGGGTTGCAAAAGAAGTAGGTAGCTTAACCTTCGGGAG6770605.753312513909334No Hit
ACACCATGGGAGTGGGTTGCACCAGAAGTAGCTAGCTTAACCTTCGGGAG4477123.8044295220916546No Hit
GCTTGTAGTAACGAATGGTCTGGTGAACCTTCTGGACTGCTTCTGAATAA2953422.5096665354370518No Hit
AAGTCATGAAAGGTGGGAGTACCCGAAGCGCCCTTAGCTAATTAAGCGGG2711342.3039592283494716No Hit
ACACCATGGGAGTGGGTTGCACCAGAAGTAGTTAGTCTAACCTTAGGGGG2651182.2528383113204367No Hit
ACACCATGGGAGTCGAACTCATTCGAAGCGGGGATGCTAAAATAGCTACC2255571.9166689963959582No Hit
GCTATCCGGGACTGAGCTGTTTCGAGAAGAGTGGGGACCGACTGTATGTG1682811.429966595505829No Hit
GCTGTCCGGAACTGAGCTGTTTCGAGAAGACTGAGGACCGATGTGCGGAG1657941.408833330769923No Hit
ACTTCTACCGATTCGAGTGGTTCGACGAATGACTAGGATTGATGGCTCAC1611111.369039571719562No Hit
GTTGTTTCCGATGGATGGCGAAATACAGGTTGTTGGACTGGCTTTCAGCC1519681.291346994526No Hit
GCTATTACCGATTGAGTGTAAAGGTGAACCTTCTCGATAGACGCAAGTTT1500061.2746749135401347No Hit
GCTTTTACCGATTGAGTGTTCAGGTGAGTCTTCTTGATAGTTGCAAAGCT1443301.2264431440825543No Hit
AAACCAACCGAGTTTTGCATTGGTGAGGCCCGTAAGGGTCGAACCTTTGT1282911.0901518561456036No Hit
ACACCATGGGAGTTGAACTCATTCGAAGCGGGGATGCTAAAGTAGCTACC1190411.011550047216319No Hit
ACACCATGGGAGTGGGGGATACCAGAAGTGGGTAGGCTAACCGCAAGGAG997100.8472850127934004No Hit
GCTATCCGGGACTGAGCTGTTTCGAGAAGAGTGGGGACCGTCTGTCATGT931020.791133579992891No Hit
GCTGTCCGGAACTGAGCTGTTTCGAGAAAACTGAGGACTAATGTATGGAG909940.7732208650498714No Hit
GCACCTACCGATTGAATGATTCGGTGAAACTTTCGGACCGTGGACAGGAC869830.7391374211995622No Hit
GCTGCCCGGAACTGAGCCATTTCGAGAAAAATCGGGACTGACTGCTTGTG793440.674225073263259No Hit
GCTCCTACCGATTTCGAGTGATGTGGTGAACTATCTGGACTGCGATTGGG775840.6592694858345519No Hit
GCTATTACCGATTGGGTGTATAGGTGAACCTTCTCGATAGTCGCAAGACT739250.6281771594699842No Hit
GCTATCCGGGACTGAGCTGTTTCGAGAAGAGTGGGGACCGTCTTTCATAT737580.6267580781628285No Hit
GCTAGTACCGATTGAATGGCTTAGTGAGGCCTCAGGATCTGCTTAGAGAA714580.6072138445912226No Hit
ACACCATGGGAGTTTGTTGCACCAGAAGTAGGTAGTCTAACCGCAAGGAG643610.5469071377835326No Hit
ACACCATGGGAGTTGAACTCATTCGAAGCGGGGATGCTAAAATAGCTACC616920.5242273293476126No Hit
GCTTGTAGTAACGAATGGTCTGGTGAACCTTCTGGACTGCTCAGCAATGA615550.5230631728261735No Hit
GCTATCCGGGACTGAGCTGTTTCGAGAAGAGTGGGGACCGACGGCATGTG603520.512840688918938No Hit
GCTGTCCGGAACTGAGCTGTTTCGAGAAGACTGAGGACTGATGCATTAGG598290.5083965001546118No Hit
GCTGTTACCGATTGATTATTTTGATGAGTATTGGGGAGTTTAATAATAGG585730.49772364912594363No Hit
GTTGTTTCCGATGGTTGGCGAAATACAGGTTGTTGGACTGGCTTCGTGCC567980.48264059930437825No Hit
GCTTGTAGTAACGAATGGTCTGGTGAACCTTCTGGACTGCTTCTGCAAAG564160.4793945570329202No Hit
GCTTGTAGTAACGAATGGTCTGGTGAACCTTCTGGACTGCTTCTGCATAA557140.47342931704715185No Hit
GCACCTACCGATTGAATGGTTCGGTGAAACTTTCGGACCGTGGACAGGAC513580.43641423816110175No Hit
GCTACTACCGATTGAGTGTTTTAGTGAGGTCCTCGGATTGCTTTCCTGGC465850.395855704753591No Hit
GCTATCCGGGACTGAACTGATTCGAGAAGAGTGGGGACTGTCGCTTCGAG455810.38732422192494215No Hit
ACACCATGGGAGTCTATTGCACCAGAAGTAGGTAGCCTAACGAAAGAGGG414030.35182169676747727No Hit
GTTGTTTCCGATGGATGGCGAAATACAGGTTGTTGGACTGGCTCCGTGCC408390.3470291107960052No Hit
ACACCATGGGAGCGGGTTCCGCCAGAAGTAGGTAGCCTAACCGCAAGGGG389560.33102833174586005No Hit
GCTTTTACCGATTGAGTGCTCAGGTGAACCTTCTCGATAGTGCAAACTAG369600.3140673360028491No Hit
GTTGTTTCCGATGGATGGCGAAATACAGGTTGTTGGACTGGCCTTACCGC363670.30902832273851766No Hit
ACACCATGGGAGTGGGTTGCACCAGAAGTAGTTAGTCTAACCCTTCGGGG349800.29724230014555353No Hit
ACACCATGGGAGTGGGTTGTACCAGAAGTAGTTAGTCTAACCCTTCGGGG345470.29356288573837735No Hit
GCTCCTACCGATACCGGGTGATCCGGTGAACCTTTTGGACCGTGAGGAAA337810.28705380620974685No Hit
GCTTTTACCGATTGAGTGTTCAGGTGAGTCTTCTTGATAGTTTTTCTAAA333050.28300899960971015No Hit
ACACCATGGGAGCGGGTTCTGCCAGAAGTGGGTAGCCTAACCGCAAGGAG314310.26708469799527995No Hit
ACTTCTACCGATTCGAGTGGTTCGACGAATGACTAGGATTGACGACTTTG312510.2655551492809804No Hit
AAGTCACCCAAGCTTGTTTTTGGTGAGGCTGCGCTTCTTGGCGCTGTCGA267290.2271294865806318No Hit
ACACCATGGGAGTGGGGGATACCAGAAGTAGGTAGGATAACCGCAAGGAG260910.2217080861377255No Hit
GCTGCTACCGATTGAATAGGAAATGTGAAATGGGAGGACGGGAGACTCGG256350.2178332293948332No Hit
GTTGTTTCCGATGGATGGCGAAATACAGGTTGTTGGACTGGCTTTACCGC246450.20942071146618546No Hit
ACACCATGGGAGTGGGTTGCACCAGAAGTAGCTAGTCTAACCTTCGGGAG241640.20533341740186267No Hit
GCTGTCCGGAACTGAGCTGTTTCGAGAAGACTGAGGACCGATGTACGGAG235310.19995450442324242No Hit
ACACCATGGGAGTTGAATTCACCCGAAGCCGGAATACTAAACTAGTTACC215300.18295102121594534No Hit
AAACCAACCGAGTTGTGTAGGGGTGAAGCCCTTTTTGGGAAGAACCTTTT214190.18200779950879395No Hit
ACGTCATGGGAGCCGAGAGCGCCCGAAGTCGTTTTAGCTAATAAAAGGCG209310.1778610229944706No Hit
GCTCCTACCGATACCGGGTGATGAGCTGAACCTTTTGGACCGAATTTCGG206590.17554970493730676No Hit
ACACCATGAGAGCCGGGGGGACCCGAAGTCGGTAGTCTAACCGCAAGGAG204900.1741136286444366No Hit
GCTATTACCGATTGAGTGTTAAGGTGAACCTTCTCGATAGAGCAATCTAA200450.17033224432297372No Hit
GGTACTACCGATTGAATGGCCTGGTGAGGCCTTCGGATTGATCTTCATGG199440.16947399754439452No Hit
GCTGTTACCGATTGAATGGTTCAATGAGTAGTAAGGAGTTTGAGGCAGCA195340.16599002547293434No Hit
GCACCTACCGATTGAATGATTCGGTGAAACTTTCGGACTGTGGTCTGGAC182820.15535116441569496No Hit
ACACCATGGGAGTGGGTTGCACCAGAAGTAGTTAGTCTAACCTTCGGGGG169800.1442874287155946No Hit
GTTGTTTCCGATGATGGCGTAATGCAGGTGATCGGACTAGCAGACGTTTT168630.14329322205129988No Hit
GCTGCTACTGATGTGCTTTTGGGTGAAATAATCGGATCATATCAACTTGG165080.1402766120869868No Hit
ACACCATGGGAATTTGTTGCACCAGAAGTAGGTAGTCTAACCGCAAGGAG163480.1389170132298316No Hit
AAACCACCCGAGTTGGGTTTCAGTAAGGATATCTCATTTTGGGGTCTTCG159830.13581542833694632No Hit
GCTGTCCGGAACTGAGCTGTTTCGAGAAGACTGAGGACCGATGTATGGAG158070.1343198695940756No Hit
GCACCTACCGATTGAATGATTCGGTGAAACTTTCGGACCGCGAATATGAC157680.13398846737264405No Hit
GCACCTACCGATTGAATGATTCGATGAATTTTTCGGACTGTGATCAGGAC152530.12961225855117575No Hit
GCTTGTAGTAACGAATGGTCTGGTGAACCTTCTGGACTGCGATAGCAATA146230.1242588380511272No Hit
ACACCATGGGAGCGGGTTCTGCCAGAAGTAGTTAGCCTAACCGCAAGGAG145310.12347706870826297No Hit
ACACCATGGGAGTTTGTTGCACCAGAAGTAGGTAGTCTAACCTTAGGGAG144040.12239788711539604No Hit
GCTATTACCGATTGAGTGTAAAGGTGAACCTTCTCGATAGTGTCACCGCT140480.11937277965822574No Hit
GTTGTTTCCGATGATGGCGTAATGCAGGTGATCGGACGAGCAGACGTTTT138220.11745234627249404No Hit
AAACCATCCGAGTTGGGTTTCAGTGAGGCTGCCTCTAATTAGGGTTGTCG138120.11736737134392183No Hit
GCTACTACCGATTGAGCGTTTTAGTGAGGTCCTCGGACTGCTTGCCTGGC138040.11729939140106409No Hit
GCTGCTACCGATTGAATAGGAAATGTGAAATGGGAGGACGGGAGACTCTG135900.115480927929619No Hit
GCTGCTACCGATGGGTGATCCGGTGAATGATTTGGACTCTGTCTATGCTA134360.11417231402960713No Hit
GCTGTTACCGATTGATTATTTTGATGAGTATTGGGGAGTTTTAATGGATT132600.11267675528673642No Hit
ACACCATGGGAGTGGGTTGCACCAGAAGTAGTTAGTCTAACCCTCGGGAG130090.11054388457957423No Hit
ACACCATGGGAGTGGAGGATACCAGAAGTAGTTAGGCTAACCGTAAGGGG128250.10898034589384574No Hit
ACACCATGGGAGCGGGTTCTGCCAGAAGTAGTTAGCCTAACCGCAAGGGG127760.10856396874384197No Hit
GCATCTACCGATGAATTTTGCGGTGAAGTCTTGGGAGTGGTGTTAATTGT127180.10807111415812322No Hit
GCACCTACCGATTGGACGATCCGGCAAGATGTTTGGAGTTGTGTCTGTTG122100.10375438778665548No Hit
ACACCATGGGAGTGGGTTGCTCCAGAAGTAGCTAGTCTAACCGCAAGGGG120560.10244577388664362No Hit
AAGTCATGAAAGTTGGGAGTACCCGAAGCGCCCTTAGCTAATTAAGCGGG120130.10208038169378317No Hit
AAACCATCCGAGTTGGGTCCAAGTGATGATGCCTCTGATTGGGGCGTTAG119750.1017574769652088No Hit
GCTATTACCGATTGGGTGTATAGGTGAACCTTCTCGATAGCCGCAAGGCT118190.1004318680794825No Hit
GCTTGTAGTAACGAATGGTCTGGTGAACCTTCTGGACTGCGACAGCAATG118070.10032989816519584No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCATGGG4816950.094.966474
CATGGGA4854050.094.9021455
CACCATG4850450.094.895212
CCGGAAC540250.094.859326
ACACCAT4828800.094.820971
CTGTCCG430700.094.812512
CCGATTG2005750.094.810548
ACCATGG4808450.094.808353
GCTATCC529700.094.74891
AGTAACG607700.094.7186057
CGATTGA1722550.094.693919
ACCGAGT354900.094.678787
CTACTAC222200.094.636582
ACCGATT2398700.094.629697
GCTATTA380400.094.600421
TACCGAT2623000.094.563576
CGGAACT543950.094.563387
CTAGTAC95950.094.554452
TACTACC242850.094.5501253
TCCGGAA436550.094.532135
Produced by FastQC (version 0.11.2)