FastQCFastQC Report
Tue 5 Apr 2016
000000000-AJHJG_l01n03.33300000050637.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJHJG_l01n03.33300000050637.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15679705
Sequences flagged as poor quality0
Sequence length251
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTACCCACGCTTTCGCACCTCAGTGTCAGTATGATGCCAGGG204846613.064442220054524No Hit
CCTGTTTGATCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCAGAA15552969.919166208803036No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG12827108.180702379285835No Hit
CCTGTTTGCTACCCATGCTTTCGCTCCTCAGCGTCAGTAACTGCCCAGTA10737216.84783929289486No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAATGTTAGACCAGAA10113406.449993797714944No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG8113845.174740213543559No Hit
CCTGTTCGCTACCCATGCTTTCGCTCCTCAGCGTCAGTAACTGCCCAGTA7727904.928600378642328No Hit
CCTGTTTGCTACCCACGCTTTCGAACCTCAGTGTCAGTATGATGCCAGAA4336322.7655622347486766No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTGTTATCCCAGGA2128691.3576084499038725No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG1970821.256924157693018No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTTGTCCAGGG1253530.7994601939258423No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA1027600.6553694728312809No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAACGTCAGGAACCGGCCAGCA894110.5702339425390975No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCAGAA743340.47407779674426276No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAATGTTAGACCAGAA713520.4550595817969789No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG667600.4257733165260443No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG654430.41737392380787774No Hit
CCTGTTCGCTACCCATGCTTTCGCTCCTCAGCGTCAGTTACTGCCCAGAG591800.37743057028177507No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTATCCCAGGA590850.3768246915359696No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAAGCCAGAG575310.36691379078879355No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGCGTCAGTATGATGCCAGGG546360.3484504332192474No Hit
CCTGTTCGCTACCCATGCTTTCGCTCCTCAGCGTCAGTTACTACCCAGAG517250.32988503291356563No Hit
CCTGTTTGCTCCCCATGCTTTCGTACCTCAGCGTCAGTATTAGGCCAGAT477510.30454016832587094No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTATTGGCCCAGGG441920.28184203720669493No Hit
CCTGTTTGCTACCCACGCTTTCGAACCTCAGTGTCAGTATGATGCCAGGA437640.27911239401506593No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGTGTCAGTATGATGCCAGAA405840.2588314002081034No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG378440.2413565816448715No Hit
CCTGTTTGATCCCCACGCTTTCGCACATCAGCGTCAGTTGCAGACCAGAA339440.21648366471180422No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG335220.21379228754622617No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG323950.20660465231967054No Hit
CCTGTTTGCTCCCCACGCTTTCGTACCTCAGCGTCAGTTTGTGTCCAGAA317000.2021721709687778No Hit
CCTGTTCGCTACCCATGCTTTCGCTCCTCAGCGTCAGTAACTGCCCAGAG291150.1856858914118601No Hit
CCTGTTTGCTACCCACGCTTTCGCTCCTCAGCGTCAGTAACTGCCCAGTA288570.1840404522916726No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAA279390.17818575030588904No Hit
CCTGTTCGCTCCCCACGCTTTCGTCCCTCAGCGTCAGTTATATCTTGGTA272320.17367673690289454No Hit
CCTGTTTGCTACCCACGCTTTCGAGCATGAACGTCAGTGTTATCCCAGGA262960.16770723683895838No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGGCCAAAA253220.16149538527670004No Hit
CCTGTTTGCTACCCACGCTCTCGCACCTCAGTGTCAGTATGATGCCAGGG247430.1578027137627908No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATCAGCGTCAGTACATTCCCAAGG243760.15546210850267908No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTTCAAGTCCAGAG222110.1416544507693225No Hit
CCTGTTTGCTACCCACGCTTTCGTTCCTCAGCGTCAGAATGAGCCCAGTA217960.1390077173008038No Hit
CCTGTTCGCTACCCACGCTTTCGCTCCTCAGCGTCAGTAACTGCCCAGTA207500.13233667342593497No Hit
ACTGTTTGCTACCCACGCTTTCGCACCTCAGTGTCAGTATGATGCCAGGG204270.13027668569019635No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTTAGCGTCAGTATCTGTCCAGTA192850.12299338539851355No Hit
CCTGTTCGCTCCCCATGCTTTCGCTCCTCAGCGTCAGTTACTGCCCAGAG191840.12234924062665718No Hit
CCTGTTTGCTACCCACGCTTTCGAATCTCAGTGTCAATATTATGCCAGGA175210.11174317373955696No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAACGTCAGTGTTATCCCAGGA170910.10900077520591107No Hit
CCCATTCGCTCCCCTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCA170000.10842040714413952No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTGTTGGTCCAGGT163980.10458104919703529No Hit
CCTGTTTGCTCCCCATGCTTTCGCTCCTCAGCGTCAGTAACTGCCCAGTA159390.10165369820414351No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTTGC8893850.0244.834723
CTGTTCG2888400.0244.698882
TTTGCTC4408400.0244.574835
TTTGATC1833850.0244.438895
TGTTTGA1899400.0244.322833
TTGCTCC4436550.0243.892786
GTTTGCT8946850.0243.807434
CCTGTTC2870900.0243.68151
CTGTTTG10835400.0243.607152
GATCCCC1857200.0242.816738
TGTTCGC2853350.0242.746083
TTTGCTA4588050.0242.72255
TGATCCC1847750.0242.692867
TGCTACC4614300.0242.557597
TTGATCC1854800.0242.153466
CTCCCCA6140350.0242.095289
TGCTCCC4469900.0242.015537
TTGCTAC4628050.0241.961356
CCTGTTT10849750.0241.320391
GCTCCCC6422200.0239.676328
Produced by FastQC (version 0.11.2)