FastQCFastQC Report
Tue 5 Apr 2016
000000000-AJHJG_l01n02.3320000005063a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJHJG_l01n02.3320000005063a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15679705
Sequences flagged as poor quality0
Sequence length12
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTAGTTCGCGT3028141.931248068761498No Hit
TAAGGTAAGGTG2698031.7207147711006041No Hit
GTCCGAAACACT2640211.6838390773295797No Hit
ATACCTTCGGTA2639411.6833288636489017No Hit
TGACCTCCAAGA2477061.5797873748262483No Hit
CCATAGGGTTCA2408241.5358962429458973No Hit
CCATACATAGCT2372481.513089691419577No Hit
TGGCACCGATTA2291001.4611244280424918No Hit
ATGTCGAGAGAA2282921.455971269867641No Hit
TACTAATCTGCG2269941.4476930528986356No Hit
AGCCGGCACATA2209731.4092930957565848No Hit
ATCGCTCGAGGA2143671.3671622010745736No Hit
CGACTGTCTTAA2135881.3621939953589688No Hit
CGTCCGAAATAC2118911.3513710876575804No Hit
TAAACCGCGTGT2103341.3414410538973787No Hit
GCAGGATAGATA2084951.3297125169127864No Hit
ATACTTCGCAGG2084951.3297125169127864No Hit
CAGGCGTATTGG2078651.3256945841774446No Hit
TATCAGGTGTGC2050671.307849860695721No Hit
TCCTCTGTCGAC2048291.306331974995703No Hit
ACCACATACATC2011771.2830407204727385No Hit
AGAGCCTACGTT2007051.2800304597567365No Hit
GACACATTTCTG2006671.2797881082584144No Hit
ACGATGCGACCA2002671.2772370398550228No Hit
ATCCCGAATTTG1986841.2671411866486009No Hit
AGTTCCCGAGTA1954381.2464392665550788No Hit
AGATGTTCTGCT1931171.2316366921443995No Hit
GGATCGCAGATC1918931.2238304228300214No Hit
TGAGCCGGAATC1905671.2153736310727785No Hit
TCGGAGTGTTGT1832821.1689122977760105No Hit
GAGGCTCATCAT1754851.1191855969229014No Hit
TGCAATGTTGCT1713031.092514176765443No Hit
GGAGACAAGGGA1704661.087176066131346No Hit
ATGGACCGAACC1696881.0822142380867497No Hit
ATCGATCTGTGG1691581.078834072452256No Hit
TCTTCCGCTACT1667371.0633937309407289No Hit
TCAACAGCATCG1645001.0491268808947618No Hit
GACTCGAATCGT1628801.038795053861026No Hit
CTGCTAACGCAA1628671.038712144137916No Hit
TCGTCGATAATC1628211.0384187712715258No Hit
GGTCACTGACAG1550970.9891576404020356No Hit
GATTGGTTGCAC1477260.9421478273985383No Hit
TAACACCACATC1442770.920151240090295No Hit
ACATTCAGCGCA1413860.9017133932047828No Hit
TATGCACCAGTG1406810.8972171351438053No Hit
GTATGCGCTGTA1385590.8836837172638133No Hit
CGCCAAATAACC1327870.8468718002028737No Hit
AATGTCCGTGAC1300000.8290972311022432No Hit
AACACAAGGAGT1187530.7573675652698824No Hit
AAGATGGATCAG1066480.6801658577122465No Hit
CAACACGCACGA1064730.6790497652857627No Hit
GTGGGATGTTTC925900.5905085586750516No Hit
ACACCTGGTGAT763180.48673109602508463No Hit
TGGCAAGACTCT759300.4842565596737949No Hit
TCTGTTGCTCTC757150.482885360406972No Hit
CCATGCGATAAC750860.4788738053426388No Hit
AATCTTGCTGCA719220.4586948542718119No Hit
CAAGCATGCCTA710130.4528975513251047No Hit
GTCTACACACAT704280.44916661378514455No Hit
GCATGGCTCTAA672520.4289111306622159No Hit
GCCTGAATTTAC647750.41311363957421393No Hit
ATGATGACCCGT642550.40979725064980493No Hit
CGGACTACAACT619340.3949946762391257No Hit
TAACGCTTGGGT599380.382264844906202No Hit
TGTAATTGTCGC593090.37825328984186884No Hit
TTATGCAGTCGT580600.37028757875227886No Hit
AATCCGTACAGC568040.36227722396562945No Hit
ATGTGGGACCCA565660.3607593382656115No Hit
CTACCGGATCAA565610.3607274499105691No Hit
ACGGCATGGCAT562310.3586228184777711No Hit
GAAGTTGGAAGT557080.3552872965403367No Hit
GAATAGAGCCAA528530.33707904581112974No Hit
ACGTGTACCCAA525340.335044568759425No Hit
CGGGTTTGACGA522750.333392751968229No Hit
GCTGATGAGCTG502640.3205672555701782No Hit
AAGAGATGTCGA502400.32041419146597466No Hit
GTACCTAATTGC494080.3151079691869203No Hit
TCACGGGAGTTG488820.31175331423646047No Hit
ACTCACGGTATG474830.3028309524955986No Hit
GTCATATCGTAC460470.2936726169274231No Hit
CGGAGCTATGGT456180.29093659606478567No Hit
TAATCCACAGCG432320.27571947303855526No Hit
ACGGGACATGCT416180.26542591203087046No Hit
GACTTTCCCTCG408870.26076383452367247No Hit
TCAAGCTCAAGC406770.25942452361189194No Hit
GAATGATGAGTG401900.2563185978307628No Hit
ATCGGCGTTACA396400.2528108787760994No Hit
GGAAACCACCAC384290.24508751918483157No Hit
CTTACACCAAGT379520.2420453701137872No Hit
TAGCTCGTAACT371280.2367901692028007No Hit
AGCTTGACAGCT364570.2325107519561114No Hit
AAGGAGCGCCTT348710.22239576573666406No Hit
CGATCCGTATTA339330.21641351033071096No Hit
TTGCGCATACTA327300.20874117210751092No Hit
CTAACCTCCGCT324040.20666205135874685No Hit
TCTACGGAGAGC317860.20272065067550696No Hit
TCCGAATTCACA300130.19141303997747405No Hit
TGGCATACGGCA299870.19124722053125362No Hit
CGCAGCGGTATA290220.18509276800807156No Hit
GAACCAAAGGAT280700.17902122520799976No Hit
CTATTTGCGACA266960.17025830524234992No Hit
TAGCGGATCACG264590.16874679721334043No Hit
GTATTACGATCC224600.14324249085043372No Hit
CCTGAACTAGTT222490.14189680226764473No Hit
CACGCCATAATG220440.14058937971090654No Hit
GCTTCGGTAGAT215820.13764289570498933No Hit
ATTGGGCTAGGC215820.13764289570498933No Hit
AATGCCTCAACT210840.1344668155427669No Hit
TATCGTTGACCA208820.1331785259990542No Hit
CAGGAAGGTTAA208200.1327831103965285No Hit
CCACCTACTCCA202600.12921161463178038No Hit
AGCTGGAAGTCC199920.12750239880150807No Hit
CTAGATTTGCCA195990.1249959740951759No Hit
CGTGACAATGTC192910.12303165142456443No Hit
CCTAGTACTGAT190650.12159029777664822No Hit
TGAGTCACTGGT185770.11847799432451056No Hit
CCAAGTCTTACA170450.10870740233952106No Hit
TGTTATCGCACA170170.10852882755128365No Hit
AGTAGAGGGATG167000.10650710584159588No Hit
TTACTGTGCGAT166180.10598413681890062No Hit
CTTGTGTCGATA158770.10125828260161782No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTCA550.0091607096.00000056
GGTACCG550.0091607096.00000052
ACCGGCT1101.8153514E-76.00000053
AAGCGGT1750.06.00000055
ACGATGC206750.06.00000051
AGGCCCA550.0091607096.00000052
AGACCAG550.0091607096.00000053
CCGACTA1101.8153514E-76.00000056
GCAGCCG550.0091607096.00000055
ATGCCCG650.0012619716.00000052
GCCCGTG550.0091607096.00000054
GCGAGCA550.0091607096.00000056
AGTCCGA1202.5695044E-86.01
GTCGAGT1001.286613E-66.03
GCGGTGA3750.06.03
GCTGCGA1202.5695044E-86.03
TCTAGAG751.751365E-46.04
ACGGGCG600.0033965676.03
GGCCACT1001.286613E-66.01
GGGAAGG1601.0913936E-116.04
Produced by FastQC (version 0.11.2)