FastQCFastQC Report
Fri 4 Dec 2015
000000000-AJHHN_l01n02.3320000003f35a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJHHN_l01n02.3320000003f35a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17973195
Sequences flagged as poor quality0
Sequence length12
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTAGCGTAGTG6709803.73322606247804No Hit
AGGCATCTTACG6255663.4805497853887415No Hit
GCGATATATCGC6095383.3913725411647735No Hit
ATGTGCACGACT6075943.3805564341787866No Hit
AATCCGTACAGC5877463.2701253171737132No Hit
GACTTGGTATTC5850133.2549193396054514No Hit
GAATCTTCGAGC5804453.2295037137247995No Hit
TAGGAACTGGCC5768143.209301406900665No Hit
CGGAGCTATGGT5750273.1993588229583003No Hit
ATGAGACTCCAC5725773.1857274124049733No Hit
TGAGTCACTGGT5652043.144705212401023No Hit
AATCAGTCTCGT5569863.0989815667164353No Hit
GGCCACGTAGTA5563743.0955764959986247No Hit
GGAGACAAGGGA5511783.0666667779434875No Hit
ACGCGCAGATAC5425163.0184727868361745No Hit
GACGGAACCCAT5408133.008997565541352No Hit
AAGAGATGTCGA5177412.8806286250163087No Hit
ACACGTAAGCCT5154352.8677984075730554No Hit
CCTGAACTAGTT5002472.783294789824514No Hit
GTCAATTGACCG4982652.7722672568789246No Hit
GAACTAGTCACC4964452.7621410661821675No Hit
AGCTGGAAGTCC4719632.625927109787659No Hit
GACAGGAGATAG4456732.479653728788899No Hit
CCAGTGTATGCA4275782.3789760251307572No Hit
CCTCGTTCGACT4194342.3336641036832906No Hit
CTAGCGAACATC3501921.948412622241065No Hit
CAAGCATGCCTA3448951.9189409562406685No Hit
TCGAGGACTGCA2943821.6378946536773236No Hit
TAGGATTGCTCG2543571.4152019159643012No Hit
TCCCAGAACAAC2238281.2453434127877654No Hit
CAGCTAGAACGC1844281.026128075726102No Hit
TACACGATCTAC1458750.8116253120271604No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCCCG550.0091591776.00010871
TGCTTCA909.150204E-66.00010871
ATGTGCC2800.06.00010871
CCTGTGT704.6964412E-46.00010871
CCGCTAG650.00126173086.00010871
TCCAGCG2150.06.00010871
ATGAGGC1507.4578566E-116.00010871
TGCGACA704.6964412E-46.00010871
TGCTGGA2200.06.00010871
TGGGGCA550.0091591776.00010871
GGCGGCA550.0091591776.00010871
ATGCGCC852.4444062E-56.00010871
TCGAGGC1451.9645086E-106.00010871
GGCGACG550.0091591776.00010871
GGCTGGA1054.829508E-76.00010871
TCTGGCG650.00126173086.00010871
GGCTACG1507.4578566E-116.00010871
CGAAGCT1054.829508E-76.00010871
ACGCGCG751.7509934E-46.00010871
GCGCGCA1001.2862765E-66.00010821
Produced by FastQC (version 0.11.2)