FastQCFastQC Report
Mon 30 Nov 2015
000000000-AJHH5_l01n03.3330000003edf0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJHH5_l01n03.3330000003edf0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17167835
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGATCAA261630415.239568646832872No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCGAGTTTGACGAA11854926.905308677535636No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGTTCAAGTTTGGGCAT9002855.2440217418212605No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGATCAG7990424.654296828924555No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGACCAG3206631.8678126857579884No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGGATAA3108771.8108107399680855No Hit
GGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATGAGGTTTAAATAA2791941.6262621349750859No Hit
AGATACCATGTTACGACTTTCTCATTCTTCAGATGATTTGGTTTACAAGA2243181.3066178699876834No Hit
AGATACCATGTTACGACTTTCTCATTCTTCAGATGATTTGGTTCACAAGA2098941.2226002871066737No Hit
CAAGCAGAAGACGGGCGGTGTGTACCGAACGGCGATAGTGTAGATCTCGG1980821.1537972027340664No Hit
GGAAACCTTGTTACGACTTGTACTTCCTCTAAATGGCCGAGTTCGAAGGT1933321.1261291828585258No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGACCAA1614910.9406602521517711No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTACTTAA1502130.8749676356978036No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCGAGTTTGACCAA1468240.8552272316223914No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACAAAGTGGTAAGC1211400.7056218795206268No Hit
GAAGACCTTGTTACGACTTCTCCTTCCTACAAACAAAAAGGTTCAGTAGG1199420.698643713665701No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATATGGTTTACTTGA1166910.6797071383782521No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGATTCA1151540.6707543496311562No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGGATGA974360.5675497230722453No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGATCGA911780.5310978349920069No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAGAAAATTCGATTTGAGCAT855870.49853111938692324No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGATCTC800250.46613332432423776No Hit
GGAAACCTTGTTACGACTTCTTCTTCCTCTAGACGTCCAAATTTACAAAG733330.42715345295431834No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAGATGATCAAGTTTGATCGT702200.4090207064548325No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCGAGTTTGATCAA699540.40747129734180226No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAGATGATCAAGTTTGTTTCA693910.4041919088807645No Hit
GGAAACCTTGTTACGACTTCACCTTCCTCTAGATGATGAGGTTTAAATAA603500.35152947357660413No Hit
CAGAAGACGGGCGGTGTGTACCGAACGGCGATAGTGTAGATCTCGGTGGT590770.3441144442499593No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAGATGACCAAGTTTGATCAA583650.33996715369177305No Hit
AGCTACCTTGTTACGACTTTTACTTCCTCTATTGAAGAAATATCGGTGAA565730.32952902914083226No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAGATGATCAAGTTTGGGTCA563340.3281368908776209No Hit
GGATACCGTGTTACGACTTCTTCATCCTCTAGATGATAAGGCTCAGGGGA544940.3174191737047799No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAGATGATCAAGTTTGTTCTC538040.31340002976496456No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAGATAACGTAGTTCGATCAA536860.3127126979027932No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAGTGAA481330.28036732645671397No Hit
GGAAACCTTGTTACGACTTGTACTTCCTCTAAATGACCAAGTTTGACCAA461710.2689389780365433No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAGTGGA455390.26525767518152404No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGGTAAGGTTTGAACAA415900.24225535718394314No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAGGTGATAAGGTTTACTTAT404030.2353412646382028No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGATCCA392440.2285902677885709No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCGAGTTTGATGAA381150.22201401632762663No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGACGAA373840.2177560536899382No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGATCAA364020.2120360546335633No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGATCTT361530.21058566790745603No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAACTGACCAAGTTTGATCAA350900.20439385630162452No Hit
GAATACCTTGTTACGACTTTTACTTCCTCTAAATGATCCAGTTTGAGCAT344040.20039801174696753No Hit
GGAAACCTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCG330810.19269174010584328No Hit
AGCTACCTTGTTACGACTTTTGCTTCCTCTATTGAAGAAATATCGGTGAA328340.1912530030723152No Hit
GGAGACCTTGTTACGACTTCTCCTTCCTACAAACAAGAAGGTTCAGTTAA322970.1881250606148067No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTTAAGCAA319460.1860805395671615No Hit
GGAAACCTTGTTACGACTTCACCTTCCTCTAAATGGCGAGGTTAAAAAAA311780.18160705761675833No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAGAACTAGGCGATAGATCAA302380.1761317021045461No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAAGCAA302340.17610840271938774No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAACGACCAAGTTTGACGAA301800.17579386101975No Hit
GGAAACCTTGTTACGACTTCACCTTCCTCTAAATGTTGAGGTTTAAATAA297210.17312025657282937No Hit
GGAAACCTTGTTACGACTTCTCCTTCTTCTAAATACTACAATTTAAAGAA296470.1726892179473999No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTAAAGCGA294360.17146017538029693No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGGGCAT288550.16807593968604662No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGAGCCAGTTTGGACGT281080.16372477950772477No Hit
GGAAACCTTGTTACGACTTCTTCTTCCTCTAGTCGTCCAAATTTACAAAA276710.16117932167917504No Hit
AAGCAGAAGACGGGCGGTGTGTACCGAACGGCGATAGTGTAGATCTCGGT265410.1545972453719412No Hit
AGAAGACGGGCGGTGTGTACCGAACGGCGATAGTGTAGATCTCGGTGGTC262590.15295463871827752No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAACGACCAAGTTTGACTAA257100.149756798105294No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGATCAG253560.14769480251878003No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGGTCAA242510.14125834736878587No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCGAGTTTGACCAG237210.1381711788353045No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTACATGACCAAGTTTGATCAA218640.12735443927554055No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTTAGTGGA211410.12314307540816884No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGAGCAAGTTTGATTCA210650.12270038709016017No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGGACAA207230.12070828965912127No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAATGGA206820.12046947096124817No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGTCCAA206530.12030055041885013No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAGTTAAAAAGATTTGAACAA203000.11824437967862575No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCGCTGACCCTACCGTGGCCGGC198820.11580959392957818No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAACTGACCGAGTTTGACGAA197450.11501158998790471No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAATAGTGAGGTTAAATCAG190700.11107981874243315No Hit
GGAAACCTTGTTACGACTTTTGCTTCCTCTAAGCGAGTAGATTCACATCA188750.10994397371596361No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTTACCCAA182700.10641994171076319No Hit
GGAGACCTTGTTACGACTTCTCCTTCCTACAAACAAGAAGGTTCAGTTGA179430.10451521697406808No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCGAGTTTGATCAG175890.10245322138755411No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAGATGATCAAGTTTGTTCCA175800.10240079777094782No Hit
GGATACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGACCAA174800.10181831314198908No Hit
GGTTACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGACCAG172170.10028637856782756No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTACG15600250.094.981359
CTTGTTA14959700.094.9748467
CCTTGTT15014900.094.949326
TTGTTAC14963850.094.936778
AAACCTT13001150.094.820553
GAAACCT12981250.094.747822
AACCTTG13175600.094.680154
ATGTTAC562400.094.6789868
CATGTTA563350.094.6120767
ACCTTGT15022950.094.55385
GGAAACC12991300.094.383861
ACCATGT566050.094.15245
AGATACC609100.094.142121
CCATGTT569450.093.848826
GTTACCT363850.093.655292
GATACCT368700.093.312252
CGTGTTA85400.092.8307347
GACCTTG566250.092.433054
TACCTTG1324950.092.124744
ATACCAT569900.091.132593
Produced by FastQC (version 0.11.2)