FastQCFastQC Report
Mon 30 Nov 2015
000000000-AJHH5_l01n02.3320000003edf3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJHH5_l01n02.3320000003edf3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17167835
Sequences flagged as poor quality0
Sequence length12
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGATGTGACT9423945.489300194229499No Hit
GGAGACAAGGGA5579313.2498623151958297No Hit
AGTTCAGACGCT5278893.074872282964043No Hit
ATTTCGACATGC5005402.9155685617901153No Hit
TGCCCATTTAAG4852702.8266231589481143No Hit
TTCAGACTACCA4744892.7638254911000715No Hit
TAATGACCACGC4654362.7110931576404362No Hit
CTTATCAAGGCG4478462.6086341114065927No Hit
TACACGATCTAC4476062.6072361482970914No Hit
TTACTGTGCGAT4349742.5336566899670223No Hit
GAGTGAGTACAA4212402.453658251025828No Hit
AGCAGTCGCGAT4194752.4433773973247064No Hit
GACTTGGTATTC3986922.3223196168882096No Hit
CCAGTGTATGCA3968132.311374730710075No Hit
CAGGGTGATACC3962462.308072042863879No Hit
AACGGATAAACG3938202.29394096576534No Hit
TGAGAACCTCCA3864102.2507788547594965No Hit
GCACACACGTTA3754702.18705503635141No Hit
ACCGTGCATAGG3708762.1602956924970447No Hit
CTAACCTCCGCT3624872.111431056973695No Hit
GGATCGCAGATC3619902.1085361083677703No Hit
TCCGACACAATT3541822.063055708538671No Hit
CACGCCATAATG3478022.025893189211103No Hit
AGGCTACACGAC3355531.9545446470099463No Hit
TGCCTACAAGCA3328551.9388292117206392No Hit
CCTCGTTCGACT3260471.8991736581811278No Hit
CAGGCGTATTGG3226991.8796720728035887No Hit
GTATGCGCTGTA3214461.872373540402736No Hit
ACACCTGGTGAT3199791.8638284908959108No Hit
AATCAGTCTCGT3097951.8045082562827521No Hit
AATCCGTACAGC2907811.6937546289325356No Hit
TATCGTTGACCA2406941.4020055528259678No Hit
TGAGTCACTGGT2259201.3159492737436025No Hit
ATCGAATGCGGC2227041.297216568076289No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAAGGC4700.06.00000055
AACTTGT806.539557E-56.00000051
GGATCGT953.429972E-66.00000051
CCTGTAG806.539557E-56.00000054
AACGGTT4700.06.00000051
ATCCGGA1900.06.00000052
TGCATCG2350.06.00000055
ACGGCTC953.429972E-66.00000052
GATGCTC806.539557E-56.00000054
GAGCCAC4550.06.00000052
TAAGGAC953.429972E-66.00000051
TGTACCA1900.06.00000056
GAGGGTG1900.06.00000051
AGGTCAG1850.06.01
GCCCCGA650.00126199386.05
CGGGTCT550.0091608326.02
CGGAAGC550.0091608326.02
CGGCGGT550.0091608326.02
TGGAGCT1054.831036E-76.06
GCCCAGT1303.6470738E-96.02
Produced by FastQC (version 0.11.2)