FastQCFastQC Report
Mon 30 Nov 2015
000000000-AJHH5_l01n01.3310000003edf6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJHH5_l01n01.3310000003edf6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17167835
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTACTACCGATTGAATGGCTTAGTGAGGTCTCCGGATTGGCTTTGGGGA244228114.225911420979989No Hit
GCTACTACCGATTGAATGGCTCAGTGAGGCCTTCGGACTGGCTCAGGGAG10868126.330512845679144No Hit
GCTACTACCGATTGAATGTTTTAGTGAGGTCTTCAGACTGGCCGGCGCAG7751184.514943206292465No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGTCTCCGGATTGACTTTGGGAA4873552.838767963461904No Hit
GCCTCAATCGACTGGATGATCCGGTGAATGATTCGGACTGGAGATATGTT2355051.3717804254292985No Hit
GCCTCAATCGACTGGATGATCCGGTGAATGATTCGGACCGGAAATATGTT2189671.2754491174921008No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGCCTCCGGATTGGTATCGGAAA2099961.2231944214282116No Hit
GTTATTTCCGATCGAATGGCTCGGTGAGGGGGTAGGAGCTCCGCGCGCGC1792021.0438241047866548No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGTCTTCGGATTGGCTTTGGGGA1410880.8218159133053177No Hit
TTCTGCTTGGTAGGTGAACCTGCAGAAGGATCATGCTGACTGACTGGATC1263150.7357654590692421No Hit
ACACCATGGGAGTGGGTTGCAAAAGAAGTAGGTAGCTTAACCTTCGGGAG1255940.7315657448944494No Hit
GCTCCTACCGATGGAACGCAGAGGCAAATTAGGTGGAGACTCGCTCCGGT1203270.7008862794871922No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGTCTCCGGATTGACTTTGGGGA1201400.6997970332310394No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGCTTTCGGATTGGATCTGAGCA1142420.6654420898150524No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGCTTTCGGATTGGACTTTGGCA931390.5425203585658879No Hit
GCTACTACCGATTGGAAGATTCGATGAGAACTTTGGATTGGGCGCGAAGT882950.5143048031391262No Hit
GCTTCTACCGATTGGACGTCCCGGTGAACAATCGGGAGGCCGTACTCATG750010.4368692965653503No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGTCTTCGGATTGGCTTTGAGGA734810.42801553020517735No Hit
TTCTGCTTGGTAGGTGAACCTGCAGAAGGATCATGCTGACTGACTCAGGC731390.42602343277413834No Hit
GCTCCTACCGATGGGACGAGAAGGCAAATTTGATGGACTGGGTCTGCTAA571770.3330472362997431No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGCTTTCGGATTGGACTCTCGTA564810.3289931432821902No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGCTTTCGGATTGGAACTAGGCA563990.328515505886444No Hit
GCTACTACCGATTGGATGGTTTAGTGAGATCCTCGGATTGGACTCGAGTC560950.32674475261440944No Hit
GCACCTACCGATTGAATGATTCGGTGAAACTTTCGGACCGTGGTTTGGAC547040.31864239142559325No Hit
GCTCCTACCGATTGAATGGTCCGGTGAAGTGTTCGGATCGCGGCGACGTG541330.31531640419423884No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGTCTCCGGATTGGCGATGGGGA540490.31482711710591343No Hit
GCTACTACCGATTGAATGTTTTAGTGAGGTCTTCGGACTGGCCGGCGCAG512100.29829037848977463No Hit
GCACCTACCGATTGAATGATTCGGTGAAACTTTCGGACCGTGATATGGAC511310.29783021563289724No Hit
GCTACTACCGATTGAATGGCTCAGTGAGGCCTTCGGACTGGCGCCAGGAG489060.2848699326385651No Hit
GCTACTACCGATTGGATGGTTTAGTGAGGTTCACGGACTGTTGCCTCTGG468940.2731503419039151No Hit
GCTACTACCGATTGGATGGTTTAGTGAGGCTCACGGATTGTTGCGCTTGG411000.23940118250204528No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGCTTTCGGATTGGATTTTGGCA406770.23693727252154972No Hit
GCTCCTACCGATTGAATGATCCGGTGAAGTGTTCGGATCGCGGCGACGTG395310.23026199867368252No Hit
GTTACTACCGATTGAATGGCTTAGTGAGGCCTCCGGATTGTGCCTTTTCA391090.22780391353947657No Hit
GCTACTACCGATTGAACGGCTCAGTGAGGCCTTCGGACTGGCTCAGGGAG388230.22613800750065455No Hit
GCTACTACCGATTGGATGACTTAGTGAGGTCTTAGGACCGACGTAAAGGA374870.2183560128577657No Hit
GCTACTACCGATTGAATGTTTTAGTGAGATCTTCGGCTTGGTTTTCCGAG353090.20566949763904419No Hit
GCTACTACCGATTGGAAGTAGACGAGAGGTCTCGGGATCGATTCCTGGGC332250.19353051797154386No Hit
GCTACTACCGATTGGATGGTTTAGTGAGGCCTCCGGACTGTTGCATTTGG327440.1907287669062523No Hit
GTTGTTTCCGATGATGGTGCAATACAGGTGATCGGACAGACGAACCTCTG320330.1865873011943556No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGCCTCCGGATTGGGCCCGAAGA316200.184181639676756No Hit
GCTCCTACTGATTGAATGCTCCGGTGAAAAGTCGGGAGAATTCTTGTGCA315310.18366322835698268No Hit
GCTGCCCGGGACTGAGCCGTTTCGAGAAAAGCGGGGACTGCTGCTGTCGG312960.18229438947892962No Hit
GCTCCTACCGATTGAATGATCCGGTGAAATCTTCGGATGGTGGCATATAG310670.1809604996786141No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGCCTCCGGATTGGCTTTGGGGA307130.17889850409210015No Hit
TTCTGGTAGGTGAACCTGCAGAAGGATCATGCTGACTGACTGGATCGCAG296720.17283483910463956No Hit
GCACCTACCGATTGAATGTTTCGGTGAAACTTTCGGACCGTGGTTTGGAC292280.17024860735206274No Hit
GCTACTACCGATTGAATGGCTTAGTGAGCTCTCTGGACTGTTGCGTGTTA289390.16856522677437194No Hit
GCTTCTACCGATTGGTCGTCCCGGTGAACAATCGGGAGGCCGTGCTCATG277360.16155793668799823No Hit
TTCTGGTAGGTGAACCTGCAGAAGGATCATGCTGACTGACTCAGGCGTAT271830.15833679668985634No Hit
GCTACTACCGATTGAACGGCTTAGTGAGGCTTTCGGATTGGCCCGGGGAG264300.15395068743379697No Hit
GCTACTACCGATTGAATGGCTCAGTGAGGCCTTCGGACTGGCGCAGGGAG263350.15339732703628617No Hit
GCTCCTACCGATTTTGAGTGATCCGGTGAACCTTCTGGACTGTGAGCAGG261930.15257019886316475No Hit
GCTCCTACCGATTTCGAGTGATCCGGTGAACCTTTTGGACTGCGCGGGGC260460.1517139464585954No Hit
GCTACTACCGATTGAATGAATTAGTGAGGACTTCGGACCGGACATCGCGG258070.150321808195384No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGTCTCCGGATTGGCTTTGGGAA257080.14974514841271483No Hit
GCTTCAACCGATTGGATGATCCGGCAAAGTGGATGGACAAGGTGCTTGCC254050.1479802199869698No Hit
GCTACTACCGATTGAATGGTTTAGTGAGATCATCTGAGTAGATGTTCATG251460.14647158479796665No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGTCTCCGGACTGGCTTTGGGGA243980.14211459977335522No Hit
GCTCCTACCGATTTCGAGTGGTCCGGTGAACCTTTCGGACCGCGCCTAGC235380.13710523196431No Hit
GCTCCTACCGATTGAATGATCCGGTGAAATCTTGGGATCGAGAGGCACAC216060.125851628932827No Hit
GCTACTACCGATTGAATGGCTCAGTGAGGCCTTGGGACTGGCTCAGAGAG215290.12540311576852875No Hit
GCTACTACCGATTGAATGTCTTAGTGAGGTCTTCAGACTGGCCGGCGCAG206480.12027142618740219No Hit
GCACCTACCGATTGAATGACTCGGTGAAAAATTGGGACTGTGAACCTGTT204730.1192520780867244No Hit
GCACCTACCGATTGAATGGTTCGGTGAAACTTTCGGACTGCGTGTTCTTT199300.11608918655147837No Hit
GCACCTACCGATTGAATGATTCGGTGAAACTTTCGGACTGTGGTCTGGAC194940.11354955356921825No Hit
GCTACTACCGATTGAATGGCTCAGTGAGGCCTTCGGACTGGCTCGAGGAG179480.104544341205516No Hit
GCACCTACCGATTGGATGATTCGGTGAAACTTTCGGACTGCGATTAGGAT179320.10445114366488262No Hit
GCTACTACCGATTGAATGGCTCAGTGAGGTCTTCGGACTGGCTCAGGGAG176620.102878435166694No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACCG10671900.094.98614
TACTACC10670850.094.975423
CTACTAC10586700.094.940672
CTACCGA13653150.094.939015
GCTACTA10527750.094.938081
TACCGAT13693300.094.889596
ACCGATT13266800.094.811217
CGATTGA11562200.094.805169
CCGATTG12927350.094.7706158
GCTCCTA1998350.094.617211
TCCTACC1941650.094.59383
CCTACCG2605850.094.573364
AATCGAC552000.094.543826
CAATCGA567550.094.522845
TCAATCG564750.094.310214
CTCCTAC2017300.094.253482
TCTACCG208550.093.9066244
GCACCTA681650.093.801341
CACCTAC685300.093.689882
ACCTACC686300.093.5464553
Produced by FastQC (version 0.11.2)