FastQCFastQC Report
Tue 8 Mar 2016
000000000-AJH7P_l01n03.3330000004af9d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJH7P_l01n03.3330000004af9d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4911240
Sequences flagged as poor quality0
Sequence length26
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGCAC52193310.627316115685652No Hit
CCTGTTTGCTCCCCACGCTTTCGAGC2868555.840785626440573No Hit
CCTGTTTGCTCCCCACGCTTTCGTGC2680635.458153134442625No Hit
CCTGTTTGCTCCCCACGCTTTCGCGC2009264.091146024221989No Hit
CCTGTTCGCTCCCCACGCTTTCGTCC1804213.67363435710737No Hit
CCTGTTTGATACCCACGCTTTCGTGC1007202.050805906451324No Hit
CCTGTTTGCTACCCACGCTTTCGCGC870971.7734217835007047No Hit
CCTGTTCGCTCCCCATGCTTTCGCTC790201.6089622987270018No Hit
CCTGTTTGCTCCCCACGCTCTCGCAC530311.0797884037432501No Hit
CCTGTTCGCTCCCCACGCTTTCGAGC524361.0676733370798415No Hit
CCTGTTTGATACCCACACTTTCGAGC509351.0371107907575277No Hit
CCTGTTCGCTCCCCACGCTTTCGCTC463620.9439978498301854No Hit
CCTGTTTGATCCCCACGCTTTCGCAC444490.9050463833980827No Hit
CCTGTTCGCTACCCATGCTTTCGCTC426030.8674591345566496No Hit
CCTGTTCGCTCCCCACGCTTTCGTGC385020.7839568011337259No Hit
CCTGTTTGCTCCCCACGCTCTCGTGC261420.5322891978400567No Hit
CCTGTTTGCTCCCCACGCTCTCGAGC256650.5225767830527525No Hit
CCTGTTTGCTCCCCACGCTCTCGCGC247200.5033352065873384No Hit
CCTGTTTGCTACCCACGCTTTCGAAC241680.49209568255674735No Hit
CCTGTTTGCTACCCACGCTTTCGGGC201540.4103647958560363No Hit
CCTGTTTGCTACCCACGCTTTCGAGC200800.4088580480693267No Hit
CCTGTTTGCTACCCACGCTTTCGCAC196890.40089671854765796No Hit
CCTGTTCGCTCCCCACGCTTTCGCGC180560.3676464599571595No Hit
CCTGTTCGCTCCCCACGCTCTCGTCC179510.3655085070165579No Hit
CCTGTTTGCTACCCACGCTTTCGTGC168280.3426425912804098No Hit
CCTGTTCGCTCCCCTAGCTTTCGCAC151830.30914799521098546No Hit
CCTGTTCGCTCCCCACGCTTTCGTGA136860.27866689471498035No Hit
CCTGTTTGCTACCCACGCTTTCGAAT118030.24032627198019235No Hit
CCTGTTCGCTCCCCACGCTTTCGCCC113400.2308989175849684No Hit
CCTGTTTGCTCCCCACGCTTACGCAC109710.22338554010799716No Hit
CCTGTTTGCTCCCCACGCTTTCCCAC104810.21340842638518986No Hit
CCTGTTTGCTCCCCATGCTTTCGTAC100830.20530456666748112No Hit
CCTGTTTGCTCCCCACGCTTTCCAGC99230.20204673361513592No Hit
CCTGTTTGCTCCCCACGCTTTCACGC95960.19538853731440534No Hit
CCTGTTCGCTCCCCATGCTTTCGCTT95360.19416684991977587No Hit
CCTGTTCGCTCCCCACGCTTTCGCTT87720.17861069709482738No Hit
CCTGTTTGCTCCCCACGCTTTCGTTC87310.1777758773751639No Hit
CCTGTTTGATACCCACACTTTCGCGC85970.17504744219382476No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGC81900.1667603293669216No Hit
CCTGTTCGCTACCCACGCTTTCGAGC79980.1628509297041073No Hit
CCTGTTTGCTCCCCACGCTTTCCTGC77260.15731261351512044No Hit
CCTGTTCGCTCCCCATGCTCTCGCTC73050.14874044029613703No Hit
CCTGTTTGCTCCCCACGCTTTCCCGC71770.14613417385426083No Hit
CCTGTTTGCTCCCCACGCTTACGAGC70250.14303923245453287No Hit
CCTGTTTGCTCCCCACACTTTCGCAC69960.14244875021379527No Hit
CCGGTTTGCTCCCCACACTTTCGCGC68820.14012754416399933No Hit
CCTGTTTGCTACCCACGCTCTCGCGC65460.13328609475407432No Hit
CCTGTTTGATACCCACGCTTTCGCGC64970.1322883833817936No Hit
CCTGTTTGATACCCACGCTCTCGTGC60340.12286102898656959No Hit
CCTGTTCGCTCCCCACGCTCTCGCTC59560.12127283537355128No Hit
CCTGTTCGCTCCCCACGCTTTCCTCC57850.11779102629885731No Hit
CCTGCTTGCTCCCCACGCTTTCGCAC57210.11648789307791922No Hit
CCTGTTTGCTCCCCATGCTTTCGCAC57020.11610102540295322No Hit
CCTGTTTGCTCCCCACGCTTACGCGC56980.11601957957664459No Hit
CCTGTTCGCTACCCACGCTTTCGCTC56090.11420740994127757No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTGGG601.9681465E-920.0000025
CTGTAAG305.470059E-420.0000022
GTCTGAT601.9681465E-920.0000024
TTTCGGG25100.020.00000219
AGCCAGT305.470059E-420.00000213
TGTATAC305.470059E-420.0000023
TTTGGGT408.260284E-620.0000026
TGTTAGA408.260284E-620.0000023
TTCGGGC22700.020.00000220
TAGCTAC1600.020.0000026
TTGGGTC408.260284E-620.0000027
TCACGTG2900.020.00000219
TCACGGG250.00448553320.019
CTGTATA356.706314E-520.02
CTCGTGT753.6379788E-1220.020
GTTAGAT451.0215117E-620.04
CTCGGGC3000.020.020
GTATGAT501.2676173E-720.04
TCTCGGG3050.020.019
CTGTTAG3900.020.02
Produced by FastQC (version 0.11.2)