FastQCFastQC Report
Tue 8 Mar 2016
000000000-AJH7P_l01n02.3320000004af90.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJH7P_l01n02.3320000004af90.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4911240
Sequences flagged as poor quality0
Sequence length12
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTGTACCCAA843461.7174074164569435No Hit
GACAGGAGATAG780931.5900872284799767No Hit
GCTGATGAGCTG729441.4852460885641916No Hit
GAGTGGTAGAGA646431.3162256375172054No Hit
GAACTAGTCACC639151.3014024971290346No Hit
TCGAGGACTGCA605871.2336395696402538No Hit
ACACCTGGTGAT585091.19132846287292No Hit
CACGGTTGTGAG562401.1451283178993492No Hit
ACACGTAAGCCT531501.0822114170759318No Hit
AAGAGATGTCGA521351.061544538650117No Hit
ATGATGACCCGT505631.029536328910825No Hit
ACACTAGATCCG482570.9825828100438992No Hit
GTCAATTGACCG475010.967189548871568No Hit
GACTTGGTATTC472610.9623027992930503No Hit
GCGATATATCGC469750.9564794227119832No Hit
CCTGAACTAGTT462270.9412490531922693No Hit
AGGCATCTTACG455720.9279122991342309No Hit
GCACACACGTTA444820.905718311465129No Hit
ACCACATACATC407440.8296071867797135No Hit
GTATGCGCTGTA396950.808248018830275No Hit
TATCGTTGACCA396010.8063340419120222No Hit
ATCCCGAATTTG395840.8059878971502106No Hit
ATTCCTGTGAGT366140.7455143711160522No Hit
ATGGTTGTTGGC363320.7397724403612937No Hit
TAGGATTGCTCG361380.7358223177853251No Hit
ATACTTCGCAGG354630.7220783345957436No Hit
TAGGAACTGGCC350580.7138319446819948No Hit
AGGCTACACGAC349360.7113478469795815No Hit
TCCAAAGTGTTC344520.7014929019962372No Hit
AGCTGGAAGTCC335830.6837987962306872No Hit
CTTCGGCAGAAT328340.668548065254396No Hit
CCTCGTTCGACT327180.6661861362914457No Hit
AGCAAACACCCG325470.6627043272167518No Hit
GACTTTCCCTCG318680.6488788982008618No Hit
GAAGTTGGAAGT309890.6309811778695401No Hit
GAACCAAAGGAT291950.5944527247701192No Hit
GTCTAATTCCGA285320.5809530790594636No Hit
GAGGCTCATCAT285190.5806883801239605No Hit
GAGGAATAGCAG282980.5761884982204087No Hit
AATGGAGCATGA281100.572360544383903No Hit
TCACGGGAGTTG264200.5379496827685065No Hit
CAGCTAGAACGC263860.5372573932448832No Hit
ACGGGACATGCT253540.5162443700572564No Hit
AATCTTGCTGCA246190.5012786994730455No Hit
ACATTCAGCGCA244470.4977765289417744No Hit
CGCCAAATAACC243120.49502773230385805No Hit
GTTGGTCAATCT235990.48051001376434466No Hit
GTCATATCGTAC233860.47617302351341No Hit
TCTGTTGCTCTC220710.44939770811444774No Hit
CCACCTACTCCA218300.4444905970793527No Hit
CAGGAAGGTTAA211650.43095022845554276No Hit
TGGCAAGACTCT207400.42229660941025077No Hit
CCTCTCGTGATC207140.4217672115392447No Hit
CTTGTGTCGATA203890.41514973815166845No Hit
TTACTGTGCGAT197870.4028921412922195No Hit
GCTTCGGTAGAT190810.3885169529487461No Hit
TACTAATCTGCG177000.3603977814156914No Hit
TACAGATGGCTC174310.35492054959643593No Hit
TAATCCACAGCG167060.3401584935779966No Hit
CTCGAGAGTACG164970.33590294915337066No Hit
AGCTTGACAGCT162160.3301813798551893No Hit
GACGGAACCCAT156790.3192472776732556No Hit
TACACGATCTAC154770.31513426344466977No Hit
CTTACACCAAGT149450.30430196854562186No Hit
TGACCTCCAAGA144300.29381581840838566No Hit
CCATACATAGCT143220.2916167810980526No Hit
GCCTGAATTTAC139450.28394051196846415No Hit
CTACCGGATCAA139440.283920150511887No Hit
ACTATTGTCACG135260.27540906166263507No Hit
ACGGCATGGCAT132710.2702168902354599No Hit
GGAGACAAGGGA132400.269585685081568No Hit
CGGGTTTGACGA125340.25521049673809465No Hit
GAATGATGAGTG123040.2505273617253484No Hit
ATCGCTCGAGGA109520.22299867243303118No Hit
CTAACCTCCGCT109040.2220213225173276No Hit
TCGGAGTGTTGT108320.22055529764377224No Hit
GTCTACACACAT107640.2191707185965255No Hit
CTCTACCTCTAC106840.2175418020703529No Hit
CGTCCGAAATAC104790.21336770347203554No Hit
CCAAGTCTTACA104190.2121460160774061No Hit
TCAAGCTCAAGC103010.2097433642013015No Hit
TGCAATGTTGCT100440.204510469860972No Hit
CGTGACAATGTC98130.19980697339164857No Hit
TTGATGCTATGC93330.19003347423461284No Hit
CTGCTAACGCAA90240.1837417841522711No Hit
TATGCACCAGTG89810.18286624151945333No Hit
CCATGCGATAAC88620.18044322818677158No Hit
CTATTTGCGACA87220.1775926242659695No Hit
GCAGGATAGATA86280.17567864734771668No Hit
ATTGGGCTAGGC86140.17539358695563645No Hit
GTATTACGATCC83110.16922406561275768No Hit
ATGTGGGACCCA75900.15454345542062697No Hit
GACTCGAATCGT73460.1495752600158005No Hit
ATGAGACTCCAC73200.1490458621447944No Hit
GTCCGAAACACT69440.1413899544717831No Hit
GGTCAGCTTAAC68590.1396592306627247No Hit
AATGTCCGTGAC67340.13711404859058No Hit
AGCCGGCACATA66210.13481320399736116No Hit
AGATGTTCTGCT61980.12620030786522346No Hit
ATACCTTCGGTA57440.11695620657919387No Hit
CGGACTACAACT56830.11571415772798724No Hit
TGGCATACGGCA56660.11536801296617555No Hit
ATGGACCGAACC54020.10999258842980592No Hit
TCGTCGATAATC53460.10885234686148508No Hit
GGTCACTGACAG53460.10885234686148508No Hit
CGACTGTCTTAA53080.10807861151155308No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTGT1507.4578566E-116.00001054
GACAGGC2800.06.00001051
AGGTAAG4500.06.00001053
GGCAGGT1405.18412E-106.00001053
AGCAAAC34350.06.00001051
ACAGTGT751.7502786E-46.00001054
GAAGCGG600.00339517746.00001054
CAACGTG1507.4578566E-116.00001053
GTCTGCG650.00126137246.00001051
GGAGACC650.00126137246.00001051
GAATGAT15650.06.00001051
TGTGGTA600.00339517746.00001052
GGCGTGT704.6948274E-46.00001053
TAGCGGA4050.06.00001051
GCTGCTA852.4430567E-56.00001053
GAGAGTC650.00126137246.00001054
AAGAAGC650.00126137246.00001052
CGACAGT909.144411E-66.00001053
CTGGTTT1202.5655027E-86.00001051
TCGGCGT704.6948274E-46.00001051
Produced by FastQC (version 0.11.2)