FastQCFastQC Report
Tue 8 Mar 2016
000000000-AJH7P_l01n01.3310000004af93.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJH7P_l01n01.3310000004af93.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4911240
Sequences flagged as poor quality0
Sequence length26
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGGAGGGTGCGAGCGTTAATCGGA3432746.989558645067233No Hit
TACGGAGGGTGCAAGCGTTAATCGGA2960036.027052231208412No Hit
TACGTAGGTGGCGAGCGTTATCCGGA2035534.144635570650182No Hit
TACGTAGGGGGCAAGCGTTATCCGGA1787623.6398547006458655No Hit
TACGGAGGGTGCAAGCGTTATCCGGA1723073.5084214984403124No Hit
TACGTAGGGGGCTAGCGTTATCCGGA1296842.6405551347521197No Hit
TACGTAGGGTGCGAGCGTTGTCCGGA1246592.538238815451902No Hit
TACGGAGGATACGAGCGTTATCCGGA1164552.3711934256929004No Hit
TACGTAGGGAGCAAGCGTTATCCGGA1161192.3643519762829754No Hit
TACGTAGGTGGCAAGCGTTGTCCGGA1155202.352155463793258No Hit
TACGTAGGGTGCGAGCGTTAATCGGA1116292.2729290362515373No Hit
TACGGAGGATGCGAGCGTTATCCGGA962271.9593218820501543No Hit
TACGTAGGTCCCGAGCGTTGTCCGGA941151.9163184857591973No Hit
TACGGAGGATCCGAGCGTTATCCGGA756121.5395704547120481No Hit
TACGTAGGTGGCAAGCGTTATCCGGA674631.3736449450647903No Hit
TACGTAGGGCGCGAGCGTTGTCCGGA527801.0746776781423837No Hit
TACGTAGGGTGCAAGCGTTAATCGGA526401.0718270742215814No Hit
TACATAGGGGGCAAGCGTTATCCGGA462600.9419209812593153No Hit
TACAGAGGGTGCAAGCGTTAATCGGA448430.9130687972894829No Hit
TACGTATGTCACAAGCGTTATCCGGA447400.9109715672620357No Hit
TACGAAGGGTGCAAGCGTTACTCGGA418240.8515975598830439No Hit
TACGTATGGTGCAAGCGTTATCCGGA409270.8333333333333334No Hit
TACGTATGTCGCAAGCGTTATCCGGA337960.6881357864816218No Hit
TACAGAGGGTGCGAGCGTTAATCGGA313390.6381076876715454No Hit
TACGTAGGGCGCAAGCGTTGTCCGGA266990.5436305291535336No Hit
TACGTATGTCGCGAGCGTTATCCGGA264310.5381736587908552No Hit
TACGAAGGGGGCTAGCGTTGCTCGGA251150.5113779819353157No Hit
TACGTATGTCACGAGCGTTATCCGGA197430.4019962372028245No Hit
TACGTAGGTGGCGAGCGTTGTCCGGA191820.39057346006303906No Hit
TACGTAGGGCGCAAGCGTTATCCGGA149410.30422052271931327No Hit
TACGGAGGGTGCAAGCGTTACTCGGA131410.26756990088042937No Hit
TACGGAGGGTGCGAGCGTTATTCGGA130110.2649229115253989No Hit
TACGGAGGGGGCTAGCGTTGTTCGGA121220.24682157662830567No Hit
TACGTAGGGGGCGAGCGTTGTCCGGA120770.2459053110823336No Hit
TACGGAGGGTGCAAGCGTTACCCGGA114510.23315903926503287No Hit
TACATAGGTCGCAAGCGTTATCCGGA100240.20410324072942881No Hit
TACGTAGGGTGCTAGCGTTATCCGGA94790.19300624689487786No Hit
TACGTAGGGTGCGAGCGTTATCCGGA92920.18919865451494938No Hit
TACGTAGGGTGCAAGCGTTGTCCGGA91420.18614443602837574No Hit
TACAGAGGATGCAAGCGTTATCCGGA64220.13076127413850677No Hit
TACGTAGGGTCCAAGCGTTAATCGGA58300.11870729184482942No Hit
TACGGAGGATGCAAGCGTTATCCGGA58050.11819825543040047No Hit
TACGGCGGGTGCGAGCGTTAATCGGA56570.11518475985698112No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTGTA408.260284E-620.0000027
GAGGAGG800.020.0000025
GAGGACA305.470059E-420.0000025
GGGGACC408.260284E-620.0000026
CGGCGGG16700.020.0000023
GGGTGAG305.470059E-420.0000027
ACACGAG305.470059E-420.0000029
ATAGGTT2400.020.0000024
ATAGGTG408.260284E-620.0000024
GGTGTAA408.260284E-620.0000028
AGGAGGG305.470059E-420.0000023
CGCGGGT601.9681465E-920.0000024
GGATGAT408.260284E-620.0000024
CCTAGGT1200.020.0000023
CTATCCG305.470059E-420.00000218
GACACGA305.470059E-420.0000028
GGGTCCA6900.020.0000027
TAGGTCC100900.020.0000025
GTGGGGA408.260284E-620.0000024
GCTATCC305.470059E-420.00000217
Produced by FastQC (version 0.11.2)