FastQCFastQC Report
Tue 1 Mar 2016
000000000-AJH22_l01n02.3320000004aefe.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJH22_l01n02.3320000004aefe.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1426853
Sequences flagged as poor quality0
Sequence length12
%GC25

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAA138430.9701770259445087No Hit
CGCCAAATAACC99830.699651610922779No Hit
GCAGGATAGATA89820.6294972222085947No Hit
CTTACACCAAGT82630.5791066073379669No Hit
CTCGAGAGTACG81400.5704862378955646No Hit
ACCACATACATC74120.5194648642852487No Hit
GAATGATGAGTG72320.5068496894914893No Hit
CTCTACCTCTAC70540.4943746833065495No Hit
CGGACTACAACT58340.4088718319266245No Hit
TCTACGGAGAGC57200.40088222122391026No Hit
ATGGTTGTTGGC56000.3924721046947373No Hit
ACTATTGTCACG52460.3676622609336771No Hit
ATGATGACCCGT50360.3529445570076245No Hit
TAACACCACATC49950.3500711005268237No Hit
TATGCACCAGTG46800.32799454463774474No Hit
TGGCACCGATTA44860.31439818958224847No Hit
AGCAAACACCCG41340.28972851443000786No Hit
ACGGCATGGCAT41310.2895182615167785No Hit
AAAAAAAAATAA36000.25230349587518824No Hit
GTCCGAAACACT34290.2403190798211168No Hit
ATTAGTTCGCGT33530.23499267268597396No Hit
TAAAAAAAAAAA33220.23282005924927093No Hit
ACGATGCGACCA30760.21557932036446642No Hit
AATGTCCGTGAC30720.21529898314682733No Hit
GACTCGAATCGT29990.21018282892491377No Hit
AGGCTACACGAC29770.20864097422789873No Hit
CTAGATTTGCCA28290.1982684971752521No Hit
ATACTTCGCAGG28000.19623605234736866No Hit
CAGGAAGGTTAA26850.18817635734024457No Hit
AATAAAAAAAAA24050.1685527521055077No Hit
AAGATGGATCAG23310.16336651357918439No Hit
AGCCGGCACATA22730.15930162392341748No Hit
AAAAAAAATAAA22650.15874094948813927No Hit
AAAAAAAAAAAT22130.155096565658831No Hit
TCAACAGCATCG21780.1526436150044889No Hit
AAAAATAAAAAA21490.15061117017660544No Hit
GTATTACGATCC21440.15026074865455658No Hit
TTGATGCTATGC20130.1410797047768761No Hit
ACACTAGATCCG19270.13505245459763549No Hit
CCATACATAGCT18450.12930554163603397No Hit
TAAGGTAAGGTG17310.1213159309333197No Hit
AAAAAAAAAAGA15300.10722898574695501No Hit
ATGGACCGAACC14790.10365468622205651No Hit
CGTACTACAACT14380.10078122974125575No Hit
ATAAAAAAAAAA14380.10078122974125575No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCATG550.00914690556.00000052
CCAGTGT1101.8050378E-76.00000051
CACCGAT5650.06.00000054
AGGGTTC550.00914690556.00000055
GCGGCAA550.00914690556.00000051
TGACACG550.00914690556.00000055
GGTTTGA1750.06.00000053
CCTTGAC1101.8050378E-76.00000056
ATGGACC1750.06.00000051
AGCGGAT1101.8050378E-76.00000052
GCATGTC1101.8050378E-76.00000054
ATTTGCC3350.06.00000055
CGAAAGT1101.8050378E-76.00000053
GGAGGTG550.00914690556.00000055
ACAAGGG1750.06.00000055
CTTCGCA3350.06.00000054
ACGATGC4350.06.00000051
GACCCTT550.00914690556.00000056
CGAGAAC1101.8050378E-76.00000053
CTGCACA550.00914690556.00000054
Produced by FastQC (version 0.11.2)