FastQCFastQC Report
Tue 10 Nov 2015
000000000-AJGTE_l01n01_filtered.3310000003c411.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJGTE_l01n01_filtered.3310000003c411.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12534411
Sequences flagged as poor quality0
Sequence length351
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAGCCCTGTAGTAGCCCGTGCGGCCGTCCCTGCCATTGAGAGTGCAATT4122413.28887412420097No Hit
CATGTACGGCGGTATTGTCTATCCGGCGACATTAAACACCTGCTGCTCTC3926003.1321774912279485No Hit
ATAGCCCGGCGGTATTGTCTATCCGGCGACATTAAACACCTGCTGCTCTC3462552.7624353469820004No Hit
CAGTACGCGGCGGTATTGTCTACCCGGCGACATTAAACGATCACTGCTTA3216832.5663990114892514No Hit
TACGGACTGAACACGGTGGCGCGTTTCTCGGTGACATCCTGCTTTACGAC3156092.517940412198068No Hit
GTCAGCCACGGCGGTATTGTCTAACCGGCGACAATAAACAGCTGCTGCTT3152242.514868867791235No Hit
GTCAGCCACTGTAGTAGCCCGTGCGGCCGTCCCTGCTATTGAGAGTGCCA3011912.4029130686715154No Hit
CAGTACGCTGTAGTAGCCCGTGCGGCCGTCCCTGCAATTGAGAGTGCCAT2891122.306546354671153No Hit
GTCAGCCACGGCGGTATTGTCTAACCGGCGACATTAAACAACTGCTGCTT2725062.1740630652688826No Hit
CATGTACTGTAGTAGCCCGTGCGGCCGTCCCTGCCATTGAGAGTGCAATT2721702.171382444695646No Hit
ATAGCCGAACACGGTGGCGCGTTTCTCGGTGACATCCTGCTTTACGACTC2686012.1429088291424305No Hit
GATTACACGGCGGTATTGTCTATCCGGCGACATTAAACACCTGCTGCTCT2660052.1221978439992113No Hit
GTCAGCCACTGTAGTAGCCCGTGCGGCCGTCCCTGCAATTGAGAGTGCCA2503041.9969346784623545No Hit
CATGTAGAACACGGTGGCGCGTTTCTCGGTGACATCCTGCTTTACGACTC2443071.9490903880525379No Hit
CATGTAGAACACGGTGGCGCGTTCCTCGGTGACATCTTGCTCTACGACTC2164841.7271174529062434No Hit
ATAGCCGAACACGGTGGCGCCTTCCTTGGTGACATCCTGCTTTACGACTC2082821.6616815899845632No Hit
CAGTACGCGGCGGTATTGTCTAACCGGCGACAATAAACAGCTGCTGCTTA2064571.6471216716924313No Hit
TACGGACTCGGCGGTATTGTCTATCCGGCGACATTAAACACCTGCTGCTC2055761.640093020725106No Hit
GATTACACTGTAGTAGCCCGTGCGGCCGTCCCTGCCATTGAGAGTGCAAT2002911.5979290929585763No Hit
GTCAGCCAGAACACGGTGGCGCCTTCCTTGGTGACATCCTGCTTTACGAC1978451.5784148134284093No Hit
ATAGCCCTGTAGTAGCCCGTGCGGCCGTCCCTGCTATTGAGAGTGCCATT1976761.5770665251043707No Hit
TACGGACTGAACACGGTGGCGCCTTCCTTGGTGACATCCTGCTTTACGAC1904681.5195608313785147No Hit
GATTACAGAACACGGTGGCGCGTTTCTCGGTGACATCCTGCTTTACGACT1891821.5093010752559495No Hit
CATGTACGGCGGTATTGTCTAACCGGCGACATTAAACAACTGCTGCTTAC1757551.40217996681296No Hit
CATGTACTGTAGTAGCCCGTGCTGAAAGACCTGTCCTGTAGATCTCGTAT1654001.3195673893252742No Hit
CATGTACTGTAGTAGCCCATGCTGAAAGACCTGTCCTGTAGATCTCGTAT1648861.315466678091216No Hit
CAGTACGGAACACGGTGGCGCCTTCCTTGGTGACATCCTGCTTTACGACT1612331.2863229073946914No Hit
CATGTACTGTAGTAGCCCGTGCGGCCGTCCCTGCAATTGAGAGTGCCATT1486471.1859113284222131No Hit
ATAGCCCGGCGGTATTGTCTAACCGGCGACAATAAACAGCTGCTGCTTAC1474241.1761541886571296No Hit
CAGTACGCTGTAGTAGCCCGTGCGGCCGTCCCTGCTATTGAGAGTGCCAT1464531.1684075143219734No Hit
GATTACACTGTAGTAGCCCGTGCGGCCGTCCCTGCAATTGAGAGTGCCAT1251570.9985072294182791No Hit
TACGGACTCTGTAGTAGCCCGTGCGGCCGTCCCTGCCATTGAGAGTGCAA1236700.9866438877742242No Hit
GATTACACGGCGGTATTGTCTAACCGGCGACATTAAACAACTGCTGCTTA1177360.9393022137218894No Hit
GATTACAGAACACGGTGGCGCGTTCCTCGGTGACATCTTGCTCTACGACT1080530.8620508773806762No Hit
TACGGACTCTGTAGTAGCCCGTGCGGCCGTCCCTGCTATTGAGAGTGCCA802200.6399981618601783No Hit
TACGGACTCGGCGGTATTGTCTAACCGGCGACAATAAACAGCTGCTGCTT759780.6061553271230694No Hit
CAGTACGCGGCGGTATTGTCTAACCGGCGACACTAAACAGCTGCTGCTTA736040.5872154662871674No Hit
GATTACACTGTAGTAGCCCGTGCGGCCGTACCTGCAATTGAGAGTGCCAT625750.49922569157816826No Hit
CAGTACGGAACACGGTGGCGCCTTTCTCGGTGACATCCTGCTTTACGACT611970.4882319560129311No Hit
TACGGACTCGGCGGTATTGTCTAACCGGCGACACTAAACAGCTGCTGCTT445760.35562899604935566No Hit
TACGGACTGAACACGGTGGCGCCTTTCTCGGTGACATCCTGCTTTACGAC402020.32073306037276106No Hit
GTCAGCCAGAACACGGTGGCGCCTTTCTCGGTGACATCTTGCTTTACGAC389920.31107963509414205No Hit
GTCAGCCACGGCGGTATTGTCTAACCGGCGACACTAAACAGCTGCTGCTT336680.26860456386821846No Hit
ATAGCCCTGTAGTAGCCCGTGCGGCCGTCCCTGCAATTGAGAGTGCCATT301280.24036231140019265No Hit
TACGGACTCTGTAGTAGCCCGTGCGGCCGTCCCTGCAATTGAGAGTGCCA268520.2142262608111382No Hit
ACTCTGATGTGCTGGAGGCGATCGAGCGTTGCTGGTGGAACGCGTTCGGT170410.1359537356801209No Hit
GTCAGCCAGAACACGGTGGCGCGTTTCTCGGTGACATCCTGCTTTACGAC148980.11885680148831883No Hit
CATGTACTGTAGTAGCCCATGCTGAAAGACCTGTCTCCACTATCTCGTAT138780.11071920331956564No Hit
CAGTACGCGGCGGTATTGTCTATCCGGCGACATTAAACACCTGCTGCTCT138670.11063144490794183No Hit
GTCAGCCACTGTAGTAGCCCGTGCGGCCGTCCCTGCCATTGAGAGTGCAA135940.1084534406921873No Hit
AGCCTCAACCCATAATAAGAGAGAACGCATCTCGTATGCCGTCTTCTGCT133130.10621161217707TruSeq Adapter, Index 18 (96% over 26bp)
GATTACAGAACACGGTGGCGCGTTTCTCGGTGACATCCTGCTTTCCGACT126290.10075463458155313No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGAC1323350.0342.731751
GCCACGG753050.0340.991155
ATAGCCC1353500.0340.399021
TGTACGG724500.0340.166533
ATAGCCG584250.0340.157751
CAGTACG1575500.0339.514471
TACGCGG736050.0338.578434
AGCCGAA591500.0336.983
GGACTGA655900.0336.610264
TAGCCCT770050.0336.241032
AGCCACG763800.0336.011264
GTACGGC732450.0335.74434
GACTCGG413500.0335.279825
GGACTCG414300.0334.923834
GATTACA1388500.0334.464751
GCCACTG694600.0333.600865
ATGTACG741050.0332.82562
CATGTAC1739850.0332.576841
ACTCGGC416350.0332.03186
GCCGAAC600750.0332.021124
Produced by FastQC (version 0.11.2)