##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AJ9MW l01n03.333000000351c2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 17382178 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.699272611291864 32.0 32.0 33.0 29.0 33.0 2 31.514444795122913 32.0 32.0 33.0 29.0 33.0 3 31.389433476058063 33.0 32.0 33.0 29.0 33.0 4 31.602313242908917 33.0 32.0 33.0 30.0 33.0 5 32.18471528711765 33.0 32.0 33.0 32.0 34.0 6 36.19639253492859 37.0 37.0 37.0 33.0 37.0 7 36.29060149999614 37.0 37.0 37.0 33.0 37.0 8 36.54902377596179 37.0 37.0 37.0 37.0 37.0 9 36.589912035189144 37.0 37.0 37.0 37.0 37.0 10-11 36.58277202085952 37.0 37.0 37.0 37.0 37.0 12-13 37.01703184146429 37.5 37.5 37.5 37.0 37.5 14-15 37.40616469351539 38.0 38.0 38.0 37.5 38.0 16-17 37.254524893255606 38.0 38.0 38.0 36.5 38.0 18-19 37.586345537365915 38.0 38.0 38.0 38.0 38.0 20-21 36.98657009495588 38.0 38.0 38.0 36.5 38.0 22-23 37.306789632461474 38.5 38.5 38.5 36.5 38.5 24-25 37.022515273977746 38.5 38.0 39.0 34.0 39.0 26-27 36.76116755909415 38.0 37.0 39.0 34.0 39.0 28-29 38.04291965023025 39.0 38.0 39.0 37.0 39.0 30-31 36.94726032031199 39.0 37.0 39.0 34.0 39.0 32-33 36.461343509426726 38.5 36.5 39.0 34.0 39.0 34-35 36.69837637722959 38.5 37.0 39.0 33.5 39.0 36-37 36.86433745529473 39.0 37.5 39.0 33.5 39.0 38-39 37.148615150529466 39.0 38.0 39.0 34.5 39.0 40-41 36.752972412317945 38.5 37.0 39.0 34.0 39.0 42-43 37.10130447979534 38.5 37.5 39.0 34.5 39.0 44-45 36.39320178978721 38.5 36.5 39.0 33.0 39.0 46-47 36.79658435208752 39.0 37.0 39.0 33.0 39.0 48-49 36.59076463260243 39.0 37.0 39.0 33.0 39.0 50-51 36.80941718005649 38.5 37.0 39.0 34.0 39.0 52-53 36.82753289029718 38.5 37.0 39.0 34.0 39.0 54-55 36.83175704448545 38.0 37.5 39.0 34.5 39.0 56-57 37.02683610765003 38.5 37.5 39.0 34.5 39.0 58-59 36.138376905356736 38.0 37.0 39.0 32.5 39.0 60-61 36.00780980956472 38.0 37.0 39.0 32.0 39.0 62-63 36.390455873826625 38.0 37.0 39.0 32.5 39.0 64-65 36.73163222123257 38.5 37.5 39.0 33.0 39.0 66-67 36.40119606990562 38.5 37.0 39.0 32.5 39.0 68-69 36.35303504543562 38.5 37.0 39.0 32.5 39.0 70-71 36.35734722081433 38.5 37.0 39.0 32.5 39.0 72-73 36.035772588452375 38.0 36.5 39.0 31.5 39.0 74-75 36.22936504274666 38.0 37.0 39.0 32.0 39.0 76-77 36.43509208109594 39.0 37.0 39.0 32.5 39.0 78-79 36.53573815663377 39.0 37.0 39.0 33.0 39.0 80-81 36.35188009810969 39.0 37.0 39.0 32.5 39.0 82-83 36.23754503031783 39.0 37.0 39.0 31.5 39.0 84-85 36.23401097952167 38.5 37.0 39.0 32.0 39.0 86-87 36.31857426037175 39.0 37.0 39.0 32.0 39.0 88-89 36.278853202400754 39.0 37.0 39.0 31.5 39.0 90-91 36.03428606587736 38.0 37.0 39.0 31.5 39.0 92-93 36.17266619292473 38.0 37.0 38.5 32.0 39.0 94-95 36.60462914946562 39.0 37.0 39.0 33.0 39.0 96-97 36.47286720916101 38.5 37.0 39.0 32.5 39.0 98-99 36.10455835856703 38.0 37.0 39.0 30.5 39.0 100-101 35.597956826814226 38.0 36.0 39.0 25.5 39.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.28353721234443796 1101 2 0.19677279107102663 1101 3 0.20946130671525864 1101 4 0.2446711065442102 1101 5 0.12056883637170301 1101 6 0.06033155232299947 1101 7 0.04769625803807287 1101 8 0.04712158973141101 1101 9 0.030436378240288775 1101 10-11 0.04462266793579772 1101 12-13 0.08152271092950514 1101 14-15 0.11692525144644605 1101 16-17 0.06778595581823055 1101 18-19 0.08968581325701308 1101 20-21 0.1691742639051128 1101 22-23 0.08907601338061255 1101 24-25 0.4639082228208977 1101 26-27 0.3635837505977264 1101 28-29 0.14684799438678908 1101 30-31 0.2642081233328071 1101 32-33 0.21024048544650498 1101 34-35 0.23936417093054985 1101 36-37 0.3098323581024047 1101 38-39 0.14178695638582184 1101 40-41 0.1740407065812235 1101 42-43 0.027897524402625606 1101 44-45 0.04725691497125695 1101 46-47 0.1959055256561868 1101 48-49 0.3191991840197659 1101 50-51 0.2283017168205035 1101 52-53 0.25471705276982703 1101 54-55 0.22527268691055014 1101 56-57 0.021376146665687656 1101 58-59 0.171788558005332 1101 60-61 0.07768063387677415 1101 62-63 0.20994596866739812 1101 64-65 0.2514353491550878 1101 66-67 0.2270343896072191 1101 68-69 0.16052540020522343 1101 70-71 0.10472753839514581 1101 72-73 0.04537630694977679 1101 74-75 0.16555502326788485 1101 76-77 0.20989031556754156 1101 78-79 0.11648795546101098 1101 80-81 0.11081904576828805 1101 82-83 0.2412584244536191 1101 84-85 0.16397930761189627 1101 86-87 0.11101060094136983 1101 88-89 0.29268141891572697 1101 90-91 0.13888129104601887 1101 92-93 0.12565447476480784 1101 94-95 0.2134524196559724 1101 96-97 0.08888459161804718 1101 98-99 0.23439458106363276 1101 100-101 0.02223984166984394 1102 1 0.08709290844298323 1102 2 0.23767436142330922 1102 3 0.13182302293988712 1102 4 0.2332464785845687 1102 5 0.08851635431608429 1102 6 0.05105061164245228 1102 7 0.03733036828425185 1102 8 0.022788905924201686 1102 9 0.027079313874693867 1102 10-11 0.03902230698491849 1102 12-13 0.03851788174353743 1102 14-15 0.1261035344365382 1102 16-17 0.04333336453218095 1102 18-19 0.10662401794845522 1102 20-21 0.3271002324375587 1102 22-23 0.2846350897506795 1102 24-25 0.05556171127155096 1102 26-27 0.2511141406706088 1102 28-29 0.15226416838665813 1102 30-31 0.21422777917284463 1102 32-33 -0.35806664325786386 1102 34-35 -0.2664422093485044 1102 36-37 -0.12489217755696558 1102 38-39 0.10617366575697673 1102 40-41 0.09063134540441808 1102 42-43 0.23468826785672547 1102 44-45 0.17299740470936342 1102 46-47 0.20390146454290203 1102 48-49 0.1519198799164485 1102 50-51 0.16677535461980852 1102 52-53 0.23179600424332847 1102 54-55 0.25650233857972893 1102 56-57 0.19705618859198637 1102 58-59 0.21649547927169266 1102 60-61 0.39696137400168396 1102 62-63 0.37357028853228513 1102 64-65 0.17972117428993784 1102 66-67 0.4490711286604565 1102 68-69 0.2587944917283309 1102 70-71 0.20225176515568677 1102 72-73 0.24819566036050844 1102 74-75 0.2149894001113637 1102 76-77 0.1842782784885273 1102 78-79 0.16728426613662606 1102 80-81 -8.938424291571323E-4 1102 82-83 0.2728659794989454 1102 84-85 0.25945042754437964 1102 86-87 0.19136970399673459 1102 88-89 0.2788584596160675 1102 90-91 0.27784464969818856 1102 92-93 0.23349815617664404 1102 94-95 0.1465421803088205 1102 96-97 0.24938604281992838 1102 98-99 0.32593371505478785 1102 100-101 0.31914614599806157 1103 1 0.06519199082380567 1103 2 -0.06683697690436574 1103 3 0.06490814572164894 1103 4 0.2137672560393007 1103 5 0.14005436016126538 1103 6 0.0794446538454423 1103 7 0.0626353475586896 1103 8 0.08525555755607428 1103 9 0.08781077914975555 1103 10-11 0.06763055980556487 1103 12-13 0.09628938146827437 1103 14-15 0.12947662181382213 1103 16-17 0.1402666507443584 1103 18-19 0.11216847015209197 1103 20-21 0.3235558085442136 1103 22-23 0.14164077618573145 1103 24-25 0.0031267085219468527 1103 26-27 0.1743000729652664 1103 28-29 0.1130444602292755 1103 30-31 0.06863429203340843 1103 32-33 -0.2889637340531479 1103 34-35 0.15331317901117103 1103 36-37 0.034793552213372436 1103 38-39 0.045616809641039424 1103 40-41 -0.0805595828647796 1103 42-43 0.08855135748187593 1103 44-45 0.059203682908162136 1103 46-47 0.20345993281293318 1103 48-49 -0.01986419415447216 1103 50-51 0.07287090504920002 1103 52-53 0.11610940567093309 1103 54-55 0.10583800097852958 1103 56-57 0.150935291512738 1103 58-59 0.1524614162807154 1103 60-61 0.461818952805487 1103 62-63 0.5030082553417472 1103 64-65 0.3913893286005745 1103 66-67 0.08326520849539776 1103 68-69 0.15257209285710616 1103 70-71 -0.045793842957841946 1103 72-73 0.33634323042316794 1103 74-75 0.20043817063252334 1103 76-77 0.2539355807832422 1103 78-79 0.11862439137538416 1103 80-81 -0.034293053380174854 1103 82-83 0.16499854175640394 1103 84-85 0.22738567262230447 1103 86-87 0.22065738642928778 1103 88-89 0.27030598562868846 1103 90-91 0.09905847957256952 1103 92-93 0.23295327988055448 1103 94-95 0.018375369567188216 1103 96-97 0.020737073906062164 1103 98-99 0.36361432163305807 1103 100-101 0.3210054608735007 2112 1 0.11924025292227114 2112 2 0.13192801998170367 2112 3 0.03755045875006857 2112 4 0.1055252467791874 2112 5 -0.01183859408277499 2112 6 0.04350986758448272 2112 7 0.03189645206405345 2112 8 -0.060160162532881145 2112 9 0.042713901751000094 2112 10-11 0.029891921396483667 2112 12-13 0.024922919485597106 2112 14-15 -1.4352847740184416E-4 2112 16-17 0.04465905040877516 2112 18-19 -0.07026990849855252 2112 20-21 0.052644144085221 2112 22-23 -0.37801640353104204 2112 24-25 -1.2091878850493387 2112 26-27 0.06311011177963621 2112 28-29 0.15680883753980623 2112 30-31 0.2051710995127891 2112 32-33 -0.13324186208041056 2112 34-35 0.07284930082568764 2112 36-37 0.24671878114650525 2112 38-39 0.18202803543658064 2112 40-41 0.16815274020351012 2112 42-43 0.1684577869046322 2112 44-45 0.14010564951668414 2112 46-47 0.13937457261478414 2112 48-49 0.15576669065545445 2112 50-51 0.182951701829964 2112 52-53 0.2019190755290694 2112 54-55 0.2959224946780381 2112 56-57 0.23327517387880903 2112 58-59 0.2595136040903441 2112 60-61 0.31939564249091035 2112 62-63 0.4315195280230668 2112 64-65 0.3978488628655157 2112 66-67 0.40661705512666657 2112 68-69 0.2926372554705452 2112 70-71 0.3197898379746036 2112 72-73 0.4284556885868511 2112 74-75 0.28018079787622696 2112 76-77 0.4282409779300238 2112 78-79 0.3838478934887348 2112 80-81 0.20725163370459398 2112 82-83 0.25482839072350316 2112 84-85 0.24322893696615466 2112 86-87 0.20615712030325284 2112 88-89 0.25395243266851253 2112 90-91 0.22609565481739935 2112 92-93 0.2888310136914143 2112 94-95 0.26296055234517013 2112 96-97 0.25276871306092374 2112 98-99 0.3743527653105332 2112 100-101 -0.07869812005476717 1113 1 0.0015153198653088396 1113 2 0.1448107150300224 1113 3 0.19988355377633837 1113 4 0.2059465123697386 1113 5 0.08692159366489705 1113 6 0.06751660570062512 1113 7 0.050016248588278245 1113 8 0.058617408904893864 1113 9 0.07869289163325988 1113 10-11 0.05647174725157811 1113 12-13 0.10144462966271561 1113 14-15 0.09697646676399785 1113 16-17 0.12973559094435672 1113 18-19 0.05380905173794304 1113 20-21 0.22508665269948835 1113 22-23 0.015699694995568336 1113 24-25 0.32047083811456645 1113 26-27 0.2673184351500595 1113 28-29 0.12571407430897352 1113 30-31 -0.03814017174666873 1113 32-33 -0.09756804057831658 1113 34-35 -0.17266748661117504 1113 36-37 0.12917481165951727 1113 38-39 0.21622971096579846 1113 40-41 0.20355041094789073 1113 42-43 0.26809987422970494 1113 44-45 0.09581223031197084 1113 46-47 0.2641601815060355 1113 48-49 0.36656948306832504 1113 50-51 0.2782887182223348 1113 52-53 0.1504504345664941 1113 54-55 0.15535808485144997 1113 56-57 0.12333129603723592 1113 58-59 0.27584775445282617 1113 60-61 0.3452349721507417 1113 62-63 0.44518123558774647 1113 64-65 0.245717156988718 1113 66-67 0.2761841374283378 1113 68-69 0.22759246386392107 1113 70-71 0.12494320536311676 1113 72-73 0.0608368760548359 1113 74-75 0.033855248942799676 1113 76-77 0.35068979287723323 1113 78-79 0.21203324058267015 1113 80-81 0.24225623953582698 1113 82-83 0.2900650709362651 1113 84-85 0.31343789474601635 1113 86-87 0.2827620968894493 1113 88-89 0.22204319475123668 1113 90-91 0.17486751309242976 1113 92-93 0.17319901247874725 1113 94-95 0.1410420470455236 1113 96-97 0.22507762355671446 1113 98-99 0.3668978233568012 1113 100-101 0.21488335439083528 2113 1 0.0858262174964537 2113 2 0.031891576149554623 2113 3 0.21395248507765174 2113 4 0.07951273048691831 2113 5 0.04893124468008381 2113 6 0.039880438539732666 2113 7 0.033059914167935744 2113 8 0.04403870022952816 2113 9 0.05858652863297209 2113 10-11 0.04274098466568432 2113 12-13 0.08063234403417852 2113 14-15 0.040736570614249956 2113 16-17 0.08125250000157536 2113 18-19 -0.0061126973609901825 2113 20-21 0.15254570027758518 2113 22-23 -0.16023193215785625 2113 24-25 0.289771148102993 2113 26-27 0.2333725962668467 2113 28-29 0.10594090992731964 2113 30-31 0.2772411173774998 2113 32-33 0.36907545495327554 2113 34-35 0.2398550195727367 2113 36-37 0.10390836959862781 2113 38-39 0.07331307947138299 2113 40-41 0.16651830504098086 2113 42-43 0.09604375211689131 2113 44-45 -0.12200599071964291 2113 46-47 -0.06498314213903456 2113 48-49 0.16937909161846676 2113 50-51 0.07549691778928747 2113 52-53 0.15919238726547036 2113 54-55 0.016390509734371506 2113 56-57 0.18591985177144466 2113 58-59 0.2643876241697072 2113 60-61 0.3514433915926034 2113 62-63 0.22954648181899273 2113 64-65 0.3237952466205769 2113 66-67 -0.027908572940766874 2113 68-69 -0.010637931467329054 2113 70-71 0.08913328701216727 2113 72-73 0.049632526777756425 2113 74-75 -0.16412985437263217 2113 76-77 0.07480049922146037 2113 78-79 0.03695258044668748 2113 80-81 0.1277646502487002 2113 82-83 0.02537830220524029 2113 84-85 0.07854249966807458 2113 86-87 -0.1303907001355853 2113 88-89 -0.37378845134975336 2113 90-91 0.004449281235821445 2113 92-93 -0.06234267937122695 2113 94-95 -0.21257735676753242 2113 96-97 0.07486000577971907 2113 98-99 0.29426215159590186 2113 100-101 -0.006000474823679269 1112 1 0.2173807189154786 1112 2 0.053308745687438375 1112 3 0.06981255304496159 1112 4 0.07497451410369393 1112 5 0.028694769278132526 1112 6 0.029011830356388657 1112 7 0.011856301980614603 1112 8 0.05809682191109289 1112 9 0.059942544537975095 1112 10-11 0.026549502481657328 1112 12-13 0.053194812114639944 1112 14-15 0.054539884383352444 1112 16-17 -0.07755029762839172 1112 18-19 0.0506659845087043 1112 20-21 0.030644050926248667 1112 22-23 0.12747289219495173 1112 24-25 0.15811996251213856 1112 26-27 0.19477180271508132 1112 28-29 0.13130138111058898 1112 30-31 0.011715140297624771 1112 32-33 -0.10414773669651822 1112 34-35 -0.07290600165759287 1112 36-37 -0.023816216552980052 1112 38-39 0.11906581939336291 1112 40-41 0.11621788454363013 1112 42-43 0.13773029208041976 1112 44-45 0.016074758955305413 1112 46-47 0.15962091112141508 1112 48-49 0.29097934063317865 1112 50-51 0.21376600440525806 1112 52-53 0.2761921136500973 1112 54-55 0.10812112420317277 1112 56-57 0.1468897256888937 1112 58-59 0.17247963211514872 1112 60-61 0.21399023892303148 1112 62-63 -0.13306467683179335 1112 64-65 0.19831922262510204 1112 66-67 0.03450212988614254 1112 68-69 0.035268690638467604 1112 70-71 -0.16172711721031163 1112 72-73 -0.1464949733296521 1112 74-75 0.13128429678022968 1112 76-77 0.21733967141596366 1112 78-79 0.18322820795130212 1112 80-81 0.17431677766253273 1112 82-83 0.17431307367887428 1112 84-85 0.1419578922420186 1112 86-87 0.18805001573657165 1112 88-89 -0.5613452165934163 1112 90-91 -0.04401647770559691 1112 92-93 -0.06845444641526655 1112 94-95 0.03154612064405171 1112 96-97 0.13406298678174977 1112 98-99 0.04490429252601302 1112 100-101 -0.12742544394224353 2114 1 0.2960507254910141 2114 2 0.19345458946333594 2114 3 0.09655674420054083 2114 4 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-0.013470392910647888 2110 74-75 0.0759742314783125 2110 76-77 0.22786714324414703 2110 78-79 0.12796915415280097 2110 80-81 -0.04923454828891494 2110 82-83 -0.08187363291951755 2110 84-85 -0.14834654631206234 2110 86-87 0.09835971863689252 2110 88-89 0.014934429642671887 2110 90-91 -0.06004712961825476 2110 92-93 -0.17921929936273528 2110 94-95 -0.08714936342853008 2110 96-97 0.04640913819012127 2110 98-99 -6.951377082202725E-4 2110 100-101 0.11009026108374798 2109 1 0.14875531390831043 2109 2 0.16625185518971008 2109 3 0.07918074694931221 2109 4 -0.0588131494203985 2109 5 0.048717870191033796 2109 6 -0.005515707484939014 2109 7 -0.01147820332052163 2109 8 0.004324475109100945 2109 9 -0.012902324628967676 2109 10-11 0.01854796559464944 2109 12-13 -0.03793437934607624 2109 14-15 -0.05009417890320833 2109 16-17 -0.0025601638797425608 2109 18-19 -0.08439821936904224 2109 20-21 -0.13736964759948478 2109 22-23 -0.09902339867193177 2109 24-25 0.06901444155791836 2109 26-27 -0.06433630404024626 2109 28-29 -0.10070830056670133 2109 30-31 -0.11764048010261519 2109 32-33 -0.18983022879417177 2109 34-35 -0.024583927394068894 2109 36-37 -0.06582457993675916 2109 38-39 -0.06284059297504285 2109 40-41 -0.2046896319547784 2109 42-43 -0.02182022191427535 2109 44-45 0.19388907293244984 2109 46-47 -0.03617505053660608 2109 48-49 -0.09078619976588698 2109 50-51 -0.03877175199123428 2109 52-53 -0.1899268605332125 2109 54-55 -0.11187008535730314 2109 56-57 -0.15357616708726596 2109 58-59 -0.21566984201610495 2109 60-61 -0.1716592572446487 2109 62-63 -0.2683308690905122 2109 64-65 -0.28371294489117105 2109 66-67 -0.06477420967178205 2109 68-69 0.04075990557912945 2109 70-71 0.07436296069263193 2109 72-73 0.03563526393833172 2109 74-75 0.25761517239286036 2109 76-77 0.0323089276761408 2109 78-79 -0.01620476498296597 2109 80-81 -0.09432309400254724 2109 82-83 -0.2320280540737798 2109 84-85 -0.11929753920022534 2109 86-87 0.1254223019093743 2109 88-89 0.18762411196048134 2109 90-91 -0.10622647779568695 2109 92-93 -0.15040765395504962 2109 94-95 -0.03984747969830238 2109 96-97 -0.06666436851435975 2109 98-99 -0.04349702878742079 2109 100-101 0.25807657678929274 2108 1 0.19435779865171554 2108 2 0.2156842799901817 2108 3 0.24430560022552683 2108 4 0.0796061135328685 2108 5 0.06954004496767396 2108 6 -0.1096466264548468 2108 7 -0.068839366883644 2108 8 -0.03870746055751084 2108 9 -0.046520821514633326 2108 10-11 -0.0029273147523767307 2108 12-13 -0.09744313552180728 2108 14-15 -0.0717722452700329 2108 16-17 -0.13667445851356064 2108 18-19 -0.14267239584120972 2108 20-21 -0.560954474584257 2108 22-23 -0.3582725282722592 2108 24-25 -0.009773929668540404 2108 26-27 -0.24230441459491203 2108 28-29 -0.05618461292210242 2108 30-31 -0.15229799226336382 2108 32-33 0.012157944861939995 2108 34-35 0.013752992284828736 2108 36-37 0.035595873081994966 2108 38-39 -0.023542568578925227 2108 40-41 -0.1920282945661853 2108 42-43 -0.20141766813333817 2108 44-45 0.1177407476123733 2108 46-47 -0.17818524416870218 2108 48-49 -0.33198729343874334 2108 50-51 -0.26498297858851316 2108 52-53 -0.29150037314354194 2108 54-55 -0.21180725016439084 2108 56-57 -0.01474909190912399 2108 58-59 -0.20564022941533722 2108 60-61 -0.11494074391870157 2108 62-63 -0.18423277553374362 2108 64-65 -0.058824164293938 2108 66-67 -0.24894733295687388 2108 68-69 -0.06404878470834774 2108 70-71 -0.17880430192064978 2108 72-73 -0.2754827147659711 2108 74-75 0.12763083327375568 2108 76-77 0.06610046872285835 2108 78-79 -0.19094588248780298 2108 80-81 -0.0088710797246776 2108 82-83 -0.13571372116892633 2108 84-85 -0.25625403923665147 2108 86-87 -0.02544657695257513 2108 88-89 0.12475632161427797 2108 90-91 -0.17729059639417244 2108 92-93 -0.24933053394314442 2108 94-95 -0.25610412195604937 2108 96-97 -0.06747541264842738 2108 98-99 0.024867021164709513 2108 100-101 0.04440355598410406 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 5.0 14 66.0 15 514.0 16 372.0 17 1641.0 18 8674.0 19 22839.0 20 28404.0 21 22322.0 22 19756.0 23 25317.0 24 36921.0 25 51550.0 26 73628.0 27 109162.0 28 158479.0 29 219594.0 30 289381.0 31 360552.0 32 427840.0 33 547634.0 34 753775.0 35 1150603.0 36 2115192.0 37 6539976.0 38 4417981.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 75.27972041248225 14.736599751768736 7.5171132179178 2.466566617831206 2 87.5129169658716 3.9420491494218965 1.9443075545538653 6.600726330152643 3 8.969698733956125 40.68979733149666 37.30942117840468 13.031082756142526 4 4.756590342130888 30.970066006688 62.158723722654315 2.1146199285267935 5 1.7061153096004427 94.33891426034184 1.9695345427943496 1.9854358872633797 6 1.5991149095355026 2.303669885327374 1.9047900671595932 94.19242513797754 7 1.8705941223246016 1.9269334372251854 1.9378814323498472 94.26459100810037 8 1.6781786494189623 2.290455200723408 94.15084806978734 1.880518080070288 9 1.6049887419171522 2.1075782332915933 94.46064814202225 1.8267848827690063 10-11 47.85456690180022 2.0539543433509886 48.443943561042815 1.6475351938059775 12-13 1.7621410849664523 48.04284883056657 48.372237932438615 1.8227721520283593 14-15 47.99308233985408 1.9187555207408415 2.2412755179471757 47.846886621457905 16-17 1.7388701231801906 46.78493972389421 1.9426046609348955 49.533585491990706 18-19 1.6564121020967568 1.86014376334197 94.65629681159633 1.8271473229649358 20-21 1.8753432394950735 24.693516543208798 43.005614716406654 30.42552550088947 22-23 9.471336100688879 12.890594032577503 10.150387943329081 67.48768192340452 24-25 1.5837198307369764 2.218102357483625 42.55145989185014 53.64671791992925 26-27 34.65770515064337 22.74128420500584 5.102720729243481 37.4982899151073 28-29 1.5426087570844116 2.2703282638113587 53.47434308865091 42.712719890453315 30-31 3.869284965324829 45.91698462643749 37.57552131844467 12.638209089793007 32-33 35.47732050609538 54.1615929833419 5.936902728760458 4.424183781802257 34-35 21.53084901098125 27.380550354506784 36.38922866858227 14.6993719659297 36-37 5.676903665351948 39.17135700715986 23.363858660289864 31.78788066719832 38-39 11.207925151842305 51.05367980928512 10.242332692715493 27.49606234615708 40-41 34.17990541806672 19.719010471530094 5.8086736886482235 40.29241042175497 42-43 22.38578502647942 9.028592389285164 65.28372048658115 3.3019020976542754 44-45 52.298785572210804 18.54887517548146 22.402825468707086 6.749513783600651 46-47 7.730394890674805 44.890858901571484 33.75490401720659 13.623842190547123 48-49 18.291229096837004 41.720962700991784 28.681434512982207 11.306373689189007 50-51 30.947937019169864 20.962974260187647 15.238326290295728 32.850762430346755 52-53 4.9129401390320595 2.5498847152526 62.683545755888595 29.853629389826754 54-55 2.845917813061171 4.440671934207555 39.68966662290537 53.0237436298259 56-57 13.658996013042785 18.52246881834946 10.006214411105443 57.81232075750231 58-59 22.881934013102388 11.531618764921173 26.431207297497473 39.15523992447897 60-61 18.077513071146782 45.492728241535666 18.955967428247483 17.473791259070065 62-63 46.979558603070345 18.02756823684581 14.326688519701042 20.66618464038281 64-65 19.327100435860224 21.522958745446054 48.329700110078264 10.820240708615456 66-67 20.063015118128465 39.042394457127294 19.818057322851026 21.076533101893215 68-69 25.06949934582421 27.884129365146297 11.814437178125779 35.23193411090371 70-71 16.12106664653877 31.271466671207715 28.340363906065164 24.267102776188345 72-73 15.597602325784491 25.553851767022522 22.385971999596368 36.46257390759662 74-75 15.155241190143146 45.11119952862064 26.005153094163457 13.728406187072759 76-77 16.140897302973194 14.213549648381232 28.49645194060261 41.14910110804296 78-79 10.315795868618997 17.206212593151445 32.25156249119069 40.22642904703887 80-81 27.302139582277896 17.046284418442845 35.68756746133885 19.964008537940412 82-83 26.672799576669853 14.659325200788992 35.21663108040891 23.451244142132246 84-85 28.155099435755403 20.812955660677275 20.94415325858474 30.08779164498258 86-87 12.589699633728294 52.75418880188663 15.542971657521859 19.113139906863225 88-89 11.65851310462935 16.20736193128387 11.219971973592722 60.91415299049405 90-91 12.813750958021487 33.40188151335236 10.788239540522483 42.99612798810368 92-93 9.252071288189546 16.76158764454029 33.59331897303088 40.393022094239285 94-95 7.858344909366363 14.627867692989913 12.496423060447317 65.0173643371964 96-97 14.821712791112828 33.447848135026575 14.151511968177982 37.57892710568262 98-99 35.05319931713966 15.497140231793738 36.15390430359187 13.29575614747473 100-101 30.57470415962833 18.16538180658373 37.58498215816223 13.674931875625711 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 1.0 5 0.5 6 1.0 7 1.0 8 1.5 9 2.5 10 2.5 11 4.5 12 9.0 13 12.5 14 15.0 15 19.5 16 36.0 17 50.0 18 59.5 19 86.5 20 141.5 21 200.0 22 290.5 23 1125.0 24 1640.0 25 3635.0 26 6995.5 27 12940.5 28 12927.5 29 14378.0 30 16039.0 31 21435.5 32 33246.0 33 89369.5 34 108890.0 35 75381.0 36 85998.5 37 335851.5 38 420601.5 39 412677.5 40 417624.5 41 288601.0 42 347359.0 43 531615.0 44 1032731.5 45 1029563.5 46 730938.0 47 815386.0 48 679607.5 49 692876.5 50 1404595.5 51 2585283.5 52 2023088.5 53 636007.5 54 761756.5 55 720049.0 56 259629.0 57 201505.0 58 193183.5 59 108173.5 60 59226.0 61 53890.0 62 51930.0 63 49839.0 64 37046.0 65 7325.0 66 2516.5 67 1379.0 68 921.0 69 691.0 70 528.5 71 373.5 72 293.5 73 249.5 74 198.5 75 170.0 76 144.0 77 108.0 78 90.5 79 89.0 80 70.5 81 56.5 82 49.5 83 41.0 84 36.0 85 33.0 86 37.5 87 36.0 88 31.5 89 31.5 90 26.5 91 31.5 92 37.5 93 41.0 94 43.5 95 40.0 96 37.5 97 39.0 98 47.5 99 68.5 100 368.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.7382178E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 4.494809304564398 #Duplication Level Percentage of deduplicated Percentage of total 1 82.59697839550616 3.712576670210256 2 7.565598717991744 0.6801184702445953 3 2.972079422832241 0.4007679073095223 4 1.2536075690015396 0.22538907861681898 5 0.7400428015482148 0.16631756350874097 6 0.49996053445390803 0.1348336357307049 7 0.309247572398958 0.0973006206082952 8 0.2875752504891795 0.10340767293289609 9 0.20676380482831272 0.08364274864104933 >10 1.9223955759721116 1.8729912719414044 >50 0.8543679917230848 2.8487775807719395 >100 0.6503109913826279 4.966856399782997 >500 0.05829744525892061 1.8148570515934368 >1k 0.057819394054908706 5.518914648635055 >5k 0.010822118949604482 3.4963931864212303 >10k+ 0.014132413608456502 73.87685549305111 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAATC 2893630 16.64710831979744 No Hit GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACAAAGTGGTAAGC 1043335 6.00232606063521 No Hit TCGTACCTTGTTACGACTTTTACCCGGTTCAAGCCATTGCGATTAAAGTG 762840 4.3886330009967685 No Hit GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAACC 728165 4.189147067761014 No Hit AGATACCTTGTTACGACTTCTTGTTGTTCCAAATGGTAGGGTTTACTTAT 668478 3.845766623722298 No Hit GGAAACCTTGTTACGACTTTTATTTCCTCTAAGTGCAAAGGTTTACTTAA 373312 2.1476710225841664 No Hit GGAAACCTTGTTACGCCTTTTCCTTCCTCTAAGTGATATGACTTACAAAA 364658 2.097884396305227 No Hit AGCTACCTTGTTACGACTTTTGCTTCCTCTAATGATTAAATATCGGTTAT 349239 2.0091785966062483 No Hit GGAAACCTTGTTACGACTTTTATTTCCTCTAGGTGTAAAGGTTTACTTAA 299432 1.7226379801196372 No Hit AGGTACCTTGTTACGACTTTTGCCCGGTTCAAGCCATTGCGATTAAACTG 294074 1.6918133044086883 No Hit GGAAACCTTGTTACGACTTCTTTTTCCTCTAAGTGCTAAGGTTTGTTTAA 252742 1.4540295238030585 No Hit GGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATGAGGTTTAAATAA 250138 1.4390486623713092 No Hit GGAAACCTTGTTACGACTTTTGCCCGGTTCAAGCCATACCGATCAAGATC 242398 1.394520295442838 No Hit GGTTACCTTGTTACGACTTCACCCCAGTCGCCAAATCCACTGTGGAAGGT 234679 1.3501127419130101 No Hit GGTTACCTTGTTACGACTTCACCCCAGTCGCTGAATCCACTGTGGAAGGT 218385 1.2563730506039001 No Hit GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGGATAA 206792 1.1896783015339043 No Hit GGAGACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGACCAG 191512 1.1017721714735633 No Hit GGAAACCTTGTTACGACTTTTATTTCCTCTAGGTGCAAAGGTTTACTTAA 191103 1.0994191867095136 No Hit GGCTACCTTGTTACGACTTCACCCCAGTCATGAAGCATACCGTGGTAAGC 180997 1.0412791768672487 No Hit GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGGATGA 135172 0.7776470819709705 No Hit GGAAACCTTGTTACGACTTTTGCTTCCTCTAAATATAAACATTCAATAGT 128763 0.7407759833088811 No Hit GAAGACCTTGTTACGACTTCTCCTTCTTCTAAATTGCAAGATTCGAACAA 123918 0.712902606336214 No Hit GGCTACCTTGTTACGACTTCACCCCAGTCATCGGCCACACCGTGGTAAGC 121366 0.6982209018915811 No Hit GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGACCAA 114251 0.657288171827489 No Hit GGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTACGAAA 94833 0.5455760492154665 No Hit GGCTACCTTGTTACGACTTCACCCCAGTCATCGACCACACCGTGGTAAGC 94053 0.5410886944087214 No Hit AGCTACCTTGTTACGACTTTTACCCGGTTCAAACCAATGCGATAAAAATG 92312 0.5310726883593069 No Hit AGCTACCTTGTTACGACTTTTGCTTCCTCTATTGATTAAATATCGGTTAT 83666 0.4813320862322316 No Hit GGCTACCTTGTTACGACTTCACCCCAGTCACGAACCCCGCCGTGGTAAGC 83270 0.479053890714961 No Hit GGAAACCTTGTTACGACTTTTAGTTCCTCTAAATGACCAAGTTTGTCCAA 71580 0.41180109880361365 No Hit GGAAACCTTGTTACGACTTCTTTTTCCTCTAAGTGTTAAGGTTTACTTAA 68415 0.39359279372239775 No Hit AGCTACCTTGTTACGACTTTTGCTTCCTCTATTGAAGAAATGTCGGTGAA 64674 0.37207074970696996 No Hit GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGATCAA 63169 0.363412456137545 No Hit GGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTACTTAA 55368 0.3185331550511104 No Hit GGCTACCTTGTTACGACTTCACCCCAGTCACGAACCCTGCCGTGGTAAGC 51392 0.2956591515746761 No Hit GGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAATGGA 50803 0.2922706233936852 No Hit GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGATCAG 49922 0.28720221366965637 No Hit GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGACCGA 49103 0.28249049112257396 No Hit GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGATCTC 47496 0.2732453896168823 No Hit GGCTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAATC 43573 0.25067629614654735 No Hit GGCTACCTTGTTACGACTTCACCCCAGTCACGAACCCTGCCGTGGTAATC 43218 0.24863397440757998 No Hit GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGACCAG 42556 0.24482547584082962 No Hit GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGGCCAAGTTTGTCCAA 41586 0.2392450474273132 No Hit CAAGCAGAAGACGGGCGGTGTGTACCGAACGGCGATAGTGTAGATCTCGG 41473 0.23859495628223343 No Hit GGAAACCTTGTTACGACTTCTCCTTCCTCTAGGTGATAAGCTTTACATAT 41170 0.23685179153038247 No Hit CGATACCTTGTTACGACTTTTGCTTCCTCTAAATGATACAATTCGAGGTA 40949 0.23558037433513795 No Hit GGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATGAGGTTTAGATAA 40158 0.23102973631957974 No Hit GGCCACCTTGTTACGACTTTTGCCTGGTTCAAGCCAATACGATCAAATGG 38964 0.22416063165386987 No Hit GGAAACCTTGTTACGACTTCATCTTCCTCTAAATGTTAAGGTTTATTCAA 37406 0.21519742807834552 No Hit GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCGAGTTTGACGAA 33932 0.19521144013138053 No Hit GGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATATGGTTTACTTGA 33875 0.19488351804934917 No Hit GGATACCTTGTTACGACTTCATCCCAGTCACCGATATCACCTTAGGCGTC 32382 0.1862942607077203 No Hit GGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTTAATGAA 30898 0.17775678053693847 No Hit GGAAACCTTGTTACGACTTTTACTTCCTCTAAACGACCAAGTTTGATCGA 27597 0.15876606487403364 No Hit GGTTACCTTGTTACGACTTCACCCCAGTCGCTGAACCCACCGTGGTCGGT 26676 0.15346753439068453 No Hit GGATACCTTGTTACGACTTCTCCTTCCTCTAAATAATAAGGTTGAATAAT 26041 0.14981436733647532 No Hit GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGATTCA 25929 0.14917002921037859 No Hit GGAAACCTTGTTACGACTTTTGCTTCCTCTACACTTTTAGATTTAAAAAA 25552 0.1470011410537851 No Hit GGCTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAACC 25300 0.14555138027006742 No Hit GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAAGC 24267 0.13960851166062158 No Hit AGCTACCTTGTTACGACTTTTACCCGGTTCAAGCCATTGCGATCGGAGTA 24093 0.13860748635757844 No Hit GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCGAGTTTGACCAA 23621 0.13589206139759932 No Hit GGAAACCTTGTTACGACTTCACCTTCCTCTAGACGATAAGGTTTGAGGAG 23432 0.13480474080981106 No Hit AGATACCTTGTTACGACTTTTGCTTCCTCTAATTACAGTGATTTGAAGTG 23205 0.13349880550066856 No Hit CGGTACCTTGTTACGACTTTTACCGGGTTCAAGCCGCGGACGATCAGAAA 23153 0.1331996485135522 No Hit GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGCCCAA 21843 0.12566319364581355 No Hit CGATACCTTGTTACGACTTTTGCTTCCTCTAAATGATACAATTTGAGGTA 21653 0.12457012003904229 No Hit GGAAACCTTGTTACGACTTCTCCTTCCTCTAGGTGATATGGTTTACTTAT 21483 0.12359210681193117 No Hit AGATACCTTGTTACGACTTAACCCTCCTTGCTGAATCTCAGTTCGAAGAC 20217 0.11630878477944478 No Hit GGCTACCTTGTTACGACTTCACCCCAGTCATGAATCACTCCGTGGTAACC 20042 0.11530200645741863 No Hit GGAAACCTTGTTACGACTTCACCTTCCTCTAAATGGCGAGGTTAAAAAAA 19521 0.11230468356727218 No Hit GGAGACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGACCAA 19088 0.10981362634763031 No Hit GAATACCTTGTTACGACTTTTACTTCCTCTAAATGATCCAGTTTGAGCAT 18859 0.10849618500052181 No Hit GGAAACCTTGTTACGACTTTTGCTTCCTCTAGATGATAAGGTTTAATCAA 18831 0.10833510046899761 No Hit GGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATGAGGTTTAAACAA 18146 0.10439428246563809 No Hit AGATACCTTGTTACGACTTAACCTTCCTTGCTAAATCTCAGTTCGACAAC 17562 0.1010345193795622 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 7.824105816888999E-4 0.0 0.0 2 7.824105816888999E-4 0.0 0.0 3 7.939166196549132E-4 0.0 0.0 4 7.939166196549132E-4 0.0 0.0 5 8.054226576209264E-4 0.0 0.0 6 8.111756766039331E-4 0.0 0.0 7 8.169286955869397E-4 0.0 0.0 8 8.341877525359596E-4 0.0 0.0 9 8.341877525359596E-4 0.0 0.0 10-11 8.428172810104695E-4 0.0 0.0 12-13 8.658293569424959E-4 0.0 0.0 14-15 9.003474708405356E-4 0.0 0.0 16-17 9.262360562640654E-4 0.0 0.0 18-19 9.578776606706018E-4 0.0 0.0 20-21 0.0010182843599921712 0.0 0.0 22-23 0.001044172945415701 0.0 0.0 24-25 0.0010671850213477274 0.0 0.0 26-27 0.0010959501162627608 0.0 0.0 28-29 0.0011678628535503432 0.0 0.0 30-31 0.001248405119312436 0.0 0.0 32-33 0.0013548359704980584 0.0 0.0 34-35 0.0014411312552431578 0.0 0.0 36-37 0.0015159205020222437 0.0 0.0 38-39 0.0015907097488013296 0.0 0.0 40-41 0.0016913875810039454 0.0 0.0 42-43 0.0018294600365961044 0.0 0.0 44-45 0.001979038530154276 0.0 0.0 46-47 0.002197653251508528 2.8189793016732427E-4 0.0 48-49 0.0023558612735412095 2.8189793016732427E-4 0.0 50-51 0.002548587409471932 2.8189793016732427E-4 0.0 52-53 0.0027528195833686664 2.8189793016732427E-4 0.0 54-55 0.002988693361671938 2.8189793016732427E-4 0.0 56-57 0.003227443649466712 2.8189793016732427E-4 0.0 58-59 0.003471946956244494 2.8189793016732427E-4 0.0 60-61 0.003759597905394824 2.8189793016732427E-4 0.0 62-63 0.004050125364036658 2.8189793016732427E-4 0.0 64-65 0.004435577635898102 2.8189793016732427E-4 0.0 66-67 0.0048008943413190225 2.8189793016732427E-4 0.0 68-69 0.0051949761416549755 2.8189793016732427E-4 0.0 70-71 0.005689735774193545 2.8189793016732427E-4 0.0 72-73 0.006181618897240611 2.8189793016732427E-4 0.0 74-75 0.006713773153168723 2.8189793016732427E-4 0.0 76-77 0.007314963636892914 2.8189793016732427E-4 0.0 78-79 0.008028337990785735 2.8189793016732427E-4 0.0 80-81 0.008756094892136072 2.8189793016732427E-4 0.0 82-83 0.009368791413826277 2.8189793016732427E-4 0.0 84-85 0.010168461052464196 2.8189793016732427E-4 0.0 86-87 0.011025660880932181 2.8189793016732427E-4 0.0 88-89 0.012078463354822393 2.8189793016732427E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGTTACG 1587830 0.0 94.97098 9 CTTGTTA 1583735 0.0 94.961006 7 CCTTGTT 1585560 0.0 94.95476 6 ACCTTGT 1584995 0.0 94.94875 5 TTGTTAC 1584580 0.0 94.91786 8 GTTACCT 601085 0.0 94.856964 2 TCGTACC 86545 0.0 94.80241 1 TACCTTG 1035045 0.0 94.69528 4 GATACCT 119930 0.0 94.6475 2 AACCTTG 500375 0.0 94.60129 4 AGATACC 93530 0.0 94.50738 1 GGTACCT 37835 0.0 94.4476 2 AAACCTT 489205 0.0 94.30867 3 AGGTACC 34510 0.0 94.25674 1 CTACCTT 185140 0.0 94.245705 3 GGAAACC 485550 0.0 94.21151 1 GGTTACC 601405 0.0 94.06169 1 TTACCTT 607285 0.0 93.980835 3 CGTACCT 89145 0.0 93.61462 2 ATACCTT 125090 0.0 93.37856 3 >>END_MODULE