FastQCFastQC Report
Mon 5 Oct 2015
000000000-AJ9MW l01n03.333000000351c2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJ9MW l01n03.333000000351c2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17382178
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAATC289363016.64710831979744No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACAAAGTGGTAAGC10433356.00232606063521No Hit
TCGTACCTTGTTACGACTTTTACCCGGTTCAAGCCATTGCGATTAAAGTG7628404.3886330009967685No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAACC7281654.189147067761014No Hit
AGATACCTTGTTACGACTTCTTGTTGTTCCAAATGGTAGGGTTTACTTAT6684783.845766623722298No Hit
GGAAACCTTGTTACGACTTTTATTTCCTCTAAGTGCAAAGGTTTACTTAA3733122.1476710225841664No Hit
GGAAACCTTGTTACGCCTTTTCCTTCCTCTAAGTGATATGACTTACAAAA3646582.097884396305227No Hit
AGCTACCTTGTTACGACTTTTGCTTCCTCTAATGATTAAATATCGGTTAT3492392.0091785966062483No Hit
GGAAACCTTGTTACGACTTTTATTTCCTCTAGGTGTAAAGGTTTACTTAA2994321.7226379801196372No Hit
AGGTACCTTGTTACGACTTTTGCCCGGTTCAAGCCATTGCGATTAAACTG2940741.6918133044086883No Hit
GGAAACCTTGTTACGACTTCTTTTTCCTCTAAGTGCTAAGGTTTGTTTAA2527421.4540295238030585No Hit
GGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATGAGGTTTAAATAA2501381.4390486623713092No Hit
GGAAACCTTGTTACGACTTTTGCCCGGTTCAAGCCATACCGATCAAGATC2423981.394520295442838No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCGCCAAATCCACTGTGGAAGGT2346791.3501127419130101No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCGCTGAATCCACTGTGGAAGGT2183851.2563730506039001No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGGATAA2067921.1896783015339043No Hit
GGAGACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGACCAG1915121.1017721714735633No Hit
GGAAACCTTGTTACGACTTTTATTTCCTCTAGGTGCAAAGGTTTACTTAA1911031.0994191867095136No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATGAAGCATACCGTGGTAAGC1809971.0412791768672487No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGGATGA1351720.7776470819709705No Hit
GGAAACCTTGTTACGACTTTTGCTTCCTCTAAATATAAACATTCAATAGT1287630.7407759833088811No Hit
GAAGACCTTGTTACGACTTCTCCTTCTTCTAAATTGCAAGATTCGAACAA1239180.712902606336214No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATCGGCCACACCGTGGTAAGC1213660.6982209018915811No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGACCAA1142510.657288171827489No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTACGAAA948330.5455760492154665No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATCGACCACACCGTGGTAAGC940530.5410886944087214No Hit
AGCTACCTTGTTACGACTTTTACCCGGTTCAAACCAATGCGATAAAAATG923120.5310726883593069No Hit
AGCTACCTTGTTACGACTTTTGCTTCCTCTATTGATTAAATATCGGTTAT836660.4813320862322316No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCACGAACCCCGCCGTGGTAAGC832700.479053890714961No Hit
GGAAACCTTGTTACGACTTTTAGTTCCTCTAAATGACCAAGTTTGTCCAA715800.41180109880361365No Hit
GGAAACCTTGTTACGACTTCTTTTTCCTCTAAGTGTTAAGGTTTACTTAA684150.39359279372239775No Hit
AGCTACCTTGTTACGACTTTTGCTTCCTCTATTGAAGAAATGTCGGTGAA646740.37207074970696996No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGATCAA631690.363412456137545No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTACTTAA553680.3185331550511104No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCACGAACCCTGCCGTGGTAAGC513920.2956591515746761No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAATGGA508030.2922706233936852No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGATCAG499220.28720221366965637No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGACCGA491030.28249049112257396No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGATCTC474960.2732453896168823No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAATC435730.25067629614654735No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCACGAACCCTGCCGTGGTAATC432180.24863397440757998No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGACCAG425560.24482547584082962No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGGCCAAGTTTGTCCAA415860.2392450474273132No Hit
CAAGCAGAAGACGGGCGGTGTGTACCGAACGGCGATAGTGTAGATCTCGG414730.23859495628223343No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAGGTGATAAGCTTTACATAT411700.23685179153038247No Hit
CGATACCTTGTTACGACTTTTGCTTCCTCTAAATGATACAATTCGAGGTA409490.23558037433513795No Hit
GGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATGAGGTTTAGATAA401580.23102973631957974No Hit
GGCCACCTTGTTACGACTTTTGCCTGGTTCAAGCCAATACGATCAAATGG389640.22416063165386987No Hit
GGAAACCTTGTTACGACTTCATCTTCCTCTAAATGTTAAGGTTTATTCAA374060.21519742807834552No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCGAGTTTGACGAA339320.19521144013138053No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATATGGTTTACTTGA338750.19488351804934917No Hit
GGATACCTTGTTACGACTTCATCCCAGTCACCGATATCACCTTAGGCGTC323820.1862942607077203No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTTAATGAA308980.17775678053693847No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAACGACCAAGTTTGATCGA275970.15876606487403364No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCGCTGAACCCACCGTGGTCGGT266760.15346753439068453No Hit
GGATACCTTGTTACGACTTCTCCTTCCTCTAAATAATAAGGTTGAATAAT260410.14981436733647532No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGATTCA259290.14917002921037859No Hit
GGAAACCTTGTTACGACTTTTGCTTCCTCTACACTTTTAGATTTAAAAAA255520.1470011410537851No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAACC253000.14555138027006742No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAAGC242670.13960851166062158No Hit
AGCTACCTTGTTACGACTTTTACCCGGTTCAAGCCATTGCGATCGGAGTA240930.13860748635757844No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCGAGTTTGACCAA236210.13589206139759932No Hit
GGAAACCTTGTTACGACTTCACCTTCCTCTAGACGATAAGGTTTGAGGAG234320.13480474080981106No Hit
AGATACCTTGTTACGACTTTTGCTTCCTCTAATTACAGTGATTTGAAGTG232050.13349880550066856No Hit
CGGTACCTTGTTACGACTTTTACCGGGTTCAAGCCGCGGACGATCAGAAA231530.1331996485135522No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGCCCAA218430.12566319364581355No Hit
CGATACCTTGTTACGACTTTTGCTTCCTCTAAATGATACAATTTGAGGTA216530.12457012003904229No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAGGTGATATGGTTTACTTAT214830.12359210681193117No Hit
AGATACCTTGTTACGACTTAACCCTCCTTGCTGAATCTCAGTTCGAAGAC202170.11630878477944478No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATGAATCACTCCGTGGTAACC200420.11530200645741863No Hit
GGAAACCTTGTTACGACTTCACCTTCCTCTAAATGGCGAGGTTAAAAAAA195210.11230468356727218No Hit
GGAGACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGACCAA190880.10981362634763031No Hit
GAATACCTTGTTACGACTTTTACTTCCTCTAAATGATCCAGTTTGAGCAT188590.10849618500052181No Hit
GGAAACCTTGTTACGACTTTTGCTTCCTCTAGATGATAAGGTTTAATCAA188310.10833510046899761No Hit
GGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATGAGGTTTAAACAA181460.10439428246563809No Hit
AGATACCTTGTTACGACTTAACCTTCCTTGCTAAATCTCAGTTCGACAAC175620.1010345193795622No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTACG15878300.094.970989
CTTGTTA15837350.094.9610067
CCTTGTT15855600.094.954766
ACCTTGT15849950.094.948755
TTGTTAC15845800.094.917868
GTTACCT6010850.094.8569642
TCGTACC865450.094.802411
TACCTTG10350450.094.695284
GATACCT1199300.094.64752
AACCTTG5003750.094.601294
AGATACC935300.094.507381
GGTACCT378350.094.44762
AAACCTT4892050.094.308673
AGGTACC345100.094.256741
CTACCTT1851400.094.2457053
GGAAACC4855500.094.211511
GGTTACC6014050.094.061691
TTACCTT6072850.093.9808353
CGTACCT891450.093.614622
ATACCTT1250900.093.378563
Produced by FastQC (version 0.11.2)