FastQCFastQC Report
Mon 5 Oct 2015
000000000-AJ9MW l01n02.332000000351c5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJ9MW l01n02.332000000351c5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17382178
Sequences flagged as poor quality0
Sequence length12
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGCGCTGTA9633745.542308909735017No Hit
CTAACCTCCGCT9587185.515522853350138No Hit
TTACTGTGCGAT9437015.429129767282328No Hit
TCCGACACAATT8962025.155867118608497No Hit
GCTGATGAGCTG8950755.149383466214648No Hit
TACACGATCTAC8947035.14724334315297No Hit
CCTCGTTCGACT8920515.131986336810036No Hit
GCACACACGTTA8400254.832679771200134No Hit
CACGCCATAATG8285124.766445263648779No Hit
CAGGCGTATTGG8241144.741143486161516No Hit
AGCTGTTGTTTG8094404.656723685604876No Hit
TATCGTTGACCA8010094.608219982559147No Hit
CCAGTGTATGCA7962054.580582479364784No Hit
GACTTGGTATTC7703524.431849679597113No Hit
AGGCTACACGAC6277153.6112563109179994No Hit
TGAGTCACTGGT6232763.585718659652432No Hit
ACACCTGGTGAT5853383.367460625475127No Hit
AATCCGTACAGC5387113.099214609354478No Hit
GGATCGCAGATC5130622.9516554254593412No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCGA1303.6470738E-96.00000055
CGAACAG550.0091608456.00000055
TCACCGG1303.6470738E-96.00000055
CGTATGT550.0091608456.00000055
CGTATAG2050.06.00000055
AGTCACA650.00126199636.00000053
ACGCATA1303.6470738E-96.00000052
GGGTATG1303.6470738E-96.00000054
GGCGTGT550.0091608456.00000053
CGCATGT650.00126199636.00000055
GAATCGC1101.8154606E-76.00000051
GAATCCG650.00126199636.00000051
ATTGTTG1101.8154606E-76.00000052
GGATCGA1101.8154606E-76.00000051
CCAGCGA650.00126199636.00000051
TTGAGCA1101.8154606E-76.00000056
GGCTTAT1101.8154606E-76.00000053
GCCGATC2050.06.00000056
ACGATCC650.00126199636.00000054
TCAGCGA550.0091608456.00000055
Produced by FastQC (version 0.11.2)