##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AJ9MW l01n02.332000000351c5.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	17382178
Sequences flagged as poor quality	0
Sequence length	12
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.95050148491173	33.0	32.0	34.0	16.0	35.0
2	31.16526340945306	33.0	32.0	34.0	16.0	35.0
3	31.301360623507595	33.0	32.0	34.0	16.0	35.0
4	31.327428242881876	33.0	32.0	34.0	16.0	35.0
5	31.33415766424668	33.0	32.0	34.0	16.0	35.0
6	33.3348211023958	36.0	33.0	37.0	18.0	37.0
7	32.77387873947672	34.0	33.0	37.0	18.0	37.0
8	33.07837867038296	35.0	33.0	37.0	18.0	37.0
9	32.80269348294558	35.0	33.0	37.0	18.0	37.0
10	32.88174962884398	35.0	33.0	37.0	18.0	37.0
11	33.34152682132239	37.0	33.0	37.0	18.0	37.0
12	33.18631928634029	37.0	33.0	37.0	18.0	37.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	-0.019796647101003373
1101	2	0.10186023236772357
1101	3	0.16113117813792144
1101	4	0.15126140917043074
1101	5	0.0962539792101822
1101	6	0.1710507695263459
1101	7	0.12494102593083056
1101	8	0.17464163858705462
1101	9	0.2191543700010783
1101	10	0.22935273136222634
1101	11	0.19196028793300712
1101	12	0.4472308121947677
1102	1	-0.07007241497074901
1102	2	0.13257793801355433
1102	3	0.13380894280256328
1102	4	0.138415142023252
1102	5	0.0698330381935186
1102	6	0.1902740701741905
1102	7	0.14922815187394178
1102	8	0.1486574359951618
1102	9	0.12263103916360052
1102	10	0.19812131058272087
1102	11	0.17241247684466288
1102	12	0.3895752217915103
1103	1	0.25970925496289254
1103	2	0.14233199483940595
1103	3	0.11743037804297174
1103	4	0.16427061852999003
1103	5	0.09760058214001077
1103	6	0.11296947559686288
1103	7	0.07448212971500823
1103	8	0.039880195564620635
1103	9	0.09028920965759113
1103	10	0.20469428122066802
1103	11	0.18330963037006853
1103	12	0.4191491696912948
2112	1	0.2469550879750919
2112	2	0.21441173847352957
2112	3	0.2639199073449312
2112	4	0.2798364204246795
2112	5	0.3191189309742697
2112	6	0.20584071533368586
2112	7	0.144029719464271
2112	8	0.2631120548130994
2112	9	0.2693386278800247
2112	10	0.3422290392185303
2112	11	0.2754482373376561
2112	12	0.5019147247833402
1113	1	0.14395262596068292
1113	2	0.16696692246082634
1113	3	0.20426790662289562
1113	4	0.16507297788544406
1113	5	0.22772812066397918
1113	6	0.14592626813094256
1113	7	0.0343747340376197
1113	8	0.22264857235600033
1113	9	0.12898949331564324
1113	10	0.20952481542705215
1113	11	0.17202059713149964
1113	12	0.48211464522213987
2113	1	-0.04404721052710414
2113	2	0.06592118844798733
2113	3	0.16778681251667038
2113	4	0.0762352914563813
2113	5	0.07367145863682012
2113	6	-0.12421110094261678
2113	7	0.056776480340872126
2113	8	0.11371243966846123
2113	9	-0.12993802954243705
2113	10	0.04676547316044122
2113	11	0.03454686878813362
2113	12	0.3596619004403081
1112	1	0.15296104710544256
1112	2	0.11945966019730037
1112	3	0.11293701330806272
1112	4	0.11052430183194772
1112	5	0.13806952897892089
1112	6	0.10339362940027286
1112	7	0.1596043330280139
1112	8	0.14516008414506842
1112	9	-0.011568457319995673
1112	10	0.08627976350944522
1112	11	0.10037104925314821
1112	12	0.289771809474793
2114	1	-0.08569840657744976
2114	2	0.07395112333875531
2114	3	0.08332698001901306
2114	4	0.02990550260415148
2114	5	0.05239983615169663
2114	6	0.051399857862151066
2114	7	0.1358693602557608
2114	8	0.12621445265573783
2114	9	0.07566792945821277
2114	10	-0.021699522025500073
2114	11	-0.09494945753299078
2114	12	0.269987346668934
1114	1	0.15886033590700777
1114	2	0.131864052984767
1114	3	0.07594978628079829
1114	4	0.08618802172904338
1114	5	0.08127129705493985
1114	6	-0.06841785061629935
1114	7	0.062293864234078455
1114	8	0.018759908039797324
1114	9	0.08255847410426043
1114	10	0.029930928106232102
1114	11	0.03997396276355403
1114	12	0.31415063598157644
1109	1	-0.1783556777819051
1109	2	-0.025473657643942715
1109	3	0.001254834048989295
1109	4	-0.0163261606483438
1109	5	0.006686450061199167
1109	6	-0.1541569098039588
1109	7	-0.01543242755332841
1109	8	-0.104331239516668
1109	9	-0.0678219004575098
1109	10	-0.11665729353788379
1109	11	-0.04451990980810905
1109	12	0.14114650727263012
1108	1	0.1199127775299722
1108	2	0.09136213045734465
1108	3	-0.02422211838489119
1108	4	-0.03984621088932627
1108	5	0.02866952782824228
1108	6	0.01999166646722017
1108	7	0.04271706481483051
1108	8	-0.11975162784430893
1108	9	0.006097539399839036
1108	10	-0.1356955700887994
1108	11	-0.014674066379797068
1108	12	0.09436190270827183
1111	1	0.00966170875141259
1111	2	-0.09215492627064137
1111	3	-0.08533419055482838
1111	4	-0.08575817174094169
1111	5	-0.06692800168275781
1111	6	-0.061693139451683976
1111	7	-0.03213407201917562
1111	8	-0.18636852588706887
1111	9	-0.08463589074810329
1111	10	-0.13419858010259844
1111	11	-0.0725239716367625
1111	12	0.06553256042286648
1110	1	0.0030401468331326953
1110	2	-0.07339184285174127
1110	3	-0.18208585047959502
1110	4	-0.1144522982942
1110	5	-0.09606455294475325
1110	6	-0.09468564379483979
1110	7	-0.13345438857898984
1110	8	-0.12390905306065747
1110	9	-0.08021538048816979
1110	10	-0.24808027061438054
1110	11	-0.1682028838389371
1110	12	0.1580452033098041
1105	1	-0.07856996660163418
1105	2	-0.19169205404058687
1105	3	-0.1096861925400141
1105	4	-0.08197784234875627
1105	5	-0.10410306901683697
1105	6	-0.027659309319581382
1105	7	-0.19102239172855917
1105	8	-0.1000602548175209
1105	9	-0.0885878627867811
1105	10	-0.210423848363952
1105	11	-0.1636962103279771
1105	12	0.10429723787501644
1104	1	0.22476767863787828
1104	2	0.10263207170580912
1104	3	0.11800325877019802
1104	4	0.12453317366383487
1104	5	0.036190319873039556
1104	6	0.198328611497395
1104	7	0.16308488318477288
1104	8	0.2165176055117115
1104	9	0.1638430041705945
1104	10	0.22154254166498788
1104	11	0.19488809718814792
1104	12	0.48859740578475197
1107	1	0.01934960498517313
1107	2	0.09948219718993911
1107	3	0.06427546679190854
1107	4	0.10244830752158407
1107	5	0.11167953018870946
1107	6	0.08241170193351621
1107	7	0.08969083242111253
1107	8	0.15038488548818663
1107	9	0.13054567228083158
1107	10	0.19863537299220013
1107	11	0.06456585089770073
1107	12	0.24531531850640675
1106	1	0.1898923002314703
1106	2	0.19982173403406023
1106	3	0.09450714850747133
1106	4	0.04226149264090395
1106	5	0.0878544747978971
1106	6	0.15112497496604504
1106	7	0.05219831763959348
1106	8	0.10338732459121758
1106	9	0.12832009988401438
1106	10	0.1158693315360253
1106	11	0.16433902016766666
1106	12	0.3146319564884621
2103	1	0.07204240321761546
2103	2	0.10945965093727494
2103	3	0.07234298191904642
2103	4	0.04986708903368964
2103	5	0.09325128218155143
2103	6	0.09544580506377542
2103	7	-0.02033167299552474
2103	8	0.10996428348419585
2103	9	0.043575693235254676
2103	10	0.05141662385263146
2103	11	0.055745116202722045
2103	12	0.31883240435321625
2102	1	0.031776071232240355
2102	2	0.025331230480148292
2102	3	0.03822697142943454
2102	4	0.006323239801410807
2102	5	0.01742798795332945
2102	6	0.024980948901287547
2102	7	-0.055001448709148804
2102	8	0.08746981150599709
2102	9	0.0025352602555130943
2102	10	-0.07607711205689327
2102	11	-0.0322837532039415
2102	12	0.2180829861964284
2101	1	-0.2262526576274979
2101	2	-0.28811043973671957
2101	3	-0.11782952204811536
2101	4	-0.0881574156544147
2101	5	-0.12055926939558859
2101	6	-0.02085051237978064
2101	7	-0.01645329776653881
2101	8	0.08169868145538572
2101	9	-0.060429098185665
2101	10	-0.1753133522059045
2101	11	0.0075631172385541845
2101	12	-6.482034235317755
2107	1	-0.3281488006812765
2107	2	-0.2616544225625752
2107	3	-0.1418785085401808
2107	4	-0.09829960119354197
2107	5	-0.08221066038079172
2107	6	0.05839499137476878
2107	7	0.011011404943083392
2107	8	0.04832514814786748
2107	9	0.03291525241230886
2107	10	0.00592759166609369
2107	11	-0.04729962589662762
2107	12	-0.006473807985841518
2106	1	0.1277163178570042
2106	2	0.04123903523853656
2106	3	0.026077673689414382
2106	4	0.023130154899480004
2106	5	-0.06109118023842086
2106	6	0.022499166272503146
2106	7	0.0783923628263068
2106	8	0.0792635358711351
2106	9	0.023769928446981226
2106	10	-0.04519500435465318
2106	11	-0.07581843249155185
2106	12	0.3678158178850879
2105	1	-0.012869678086200764
2105	2	-0.0684001084873529
2105	3	-0.013565305946830364
2105	4	-0.040570797087919885
2105	5	-0.02984589487746092
2105	6	0.0399258136811369
2105	7	0.0422822980212203
2105	8	-0.06384221131506251
2105	9	-0.0200390678619371
2105	10	0.03135925849927901
2105	11	-0.09140798110819759
2105	12	0.2670862922302959
2104	1	-0.15791899951579325
2104	2	-0.2103325689493829
2104	3	-0.24185457569426916
2104	4	-0.1836346986756645
2104	5	-0.25875168969832174
2104	6	-0.05045512647237871
2104	7	-0.14925108667834053
2104	8	-0.16995896522889353
2104	9	-0.10954874701295125
2104	10	-0.18042148784733314
2104	11	-0.1771298746582204
2104	12	0.18032157540068994
2111	1	-0.06647749965017269
2111	2	-0.1553469919645778
2111	3	-0.12743921402310576
2111	4	-0.14567022120681017
2111	5	-0.1825998353184204
2111	6	-0.06375586674325717
2111	7	-0.06358753442434306
2111	8	-0.16179120576458672
2111	9	-0.10407827048319973
2111	10	-0.06619086205791547
2111	11	-0.10567292517577442
2111	12	0.2500559277980656
2110	1	-0.15948630445149092
2110	2	-0.11933561763700595
2110	3	-0.20724381096972522
2110	4	-0.17711735418022911
2110	5	-0.21050317496723636
2110	6	-0.23618208864076706
2110	7	-0.19425870157329683
2110	8	-0.31990766970169915
2110	9	-0.24958400138569203
2110	10	-0.162039491646496
2110	11	-0.14355986291570133
2110	12	-0.04831820573231482
2109	1	-0.28205672650715385
2109	2	-0.18906333600774516
2109	3	-0.17090267387246527
2109	4	-0.18005182486422555
2109	5	-0.12394680526471191
2109	6	-0.23818669779738855
2109	7	-0.163477423008473
2109	8	-0.29162117767437223
2109	9	-0.16242660854219082
2109	10	-0.13113503845617913
2109	11	-0.21850976735538552
2109	12	-0.128612325896313
2108	1	-0.05084637110774537
2108	2	-0.14371693501463056
2108	3	-0.3132052771782057
2108	4	-0.2984105464317892
2108	5	-0.20110221110310889
2108	6	-0.5337042202197253
2108	7	-0.38657251769542
2108	8	-0.48825612706991706
2108	9	-0.3513582788512224
2108	10	-0.2685216294399595
2108	11	-0.20689558978637734
2108	12	-0.0222407875484123
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	34.0
15	2718.0
16	89768.0
17	188932.0
18	256526.0
19	311419.0
20	290538.0
21	300628.0
22	298457.0
23	276824.0
24	251986.0
25	239258.0
26	229278.0
27	226824.0
28	242264.0
29	266560.0
30	318941.0
31	429722.0
32	721761.0
33	1917585.0
34	4267444.0
35	5210928.0
36	1043783.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.52478176210139	17.187564182118027	28.121826850467187	29.165827205313395
2	16.837805577391464	30.02449750825213	19.910176953679446	33.22751996067696
3	10.038420966396842	40.17115578624909	20.82562045166267	28.9648027956914
4	31.20140065301368	12.589256651266604	21.317271057746616	34.892071637973096
5	21.96485963956876	17.5602102337233	25.965077563927835	34.50985256278011
6	33.62244938465134	5.207839892100978	26.742086060791692	34.42762466245599
7	20.652090931993154	26.938374865242714	42.81511458547453	9.594419617289594
8	20.069793329109466	27.250251795272824	17.63410302403433	35.04585185158338
9	19.96983922267969	21.911109183210527	31.2932073299445	26.825844264165283
10	35.17148426393977	23.997297691923304	28.15834126195233	12.672876782184602
11	13.395467472488201	20.279967216996628	42.56822706567612	23.756338244839053
12	21.28793763865136	23.08727497136866	32.64791285924657	22.976874530733408
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	894.0
1	894.0
2	894.0
3	894.0
4	3946.0
5	6998.0
6	6998.0
7	6998.0
8	6998.0
9	6998.0
10	6998.0
11	6998.0
12	6998.0
13	17888.0
14	28778.0
15	28778.0
16	28778.0
17	28778.0
18	28778.0
19	28778.0
20	28778.0
21	58301.5
22	87825.0
23	87825.0
24	87825.0
25	87825.0
26	87825.0
27	87825.0
28	87825.0
29	190794.5
30	293764.0
31	293764.0
32	293764.0
33	293764.0
34	293764.0
35	293764.0
36	293764.0
37	293764.0
38	2963198.0
39	5632632.0
40	5632632.0
41	5632632.0
42	5632632.0
43	5632632.0
44	5632632.0
45	5632632.0
46	5737862.0
47	5843092.0
48	5843092.0
49	5843092.0
50	5843092.0
51	5843092.0
52	5843092.0
53	5843092.0
54	5508966.5
55	5174841.0
56	5174841.0
57	5174841.0
58	5174841.0
59	5174841.0
60	5174841.0
61	5174841.0
62	5174841.0
63	2703144.5
64	231448.0
65	231448.0
66	231448.0
67	231448.0
68	231448.0
69	231448.0
70	231448.0
71	146733.0
72	62018.0
73	62018.0
74	62018.0
75	62018.0
76	62018.0
77	62018.0
78	62018.0
79	38883.5
80	15749.0
81	15749.0
82	15749.0
83	15749.0
84	15749.0
85	15749.0
86	15749.0
87	15749.0
88	9489.0
89	3229.0
90	3229.0
91	3229.0
92	3229.0
93	3229.0
94	3229.0
95	3229.0
96	2069.5
97	910.0
98	910.0
99	910.0
100	910.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	1.1506037966013235E-5
3	1.1506037966013235E-5
4	0.0
5	0.0
6	0.0
7	5.753018983006618E-6
8	5.753018983006618E-6
9	0.0
10	0.0
11	0.0
12	5.753018983006618E-6
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
12	1.7382178E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	2.9197038617747495
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.65922143745462	2.092237055627082
2	11.902965691133925	0.6950626978995215
3	4.46858607691524	0.3914084407632691
4	2.437467769100994	0.28466736233582624
5	1.581102777639426	0.23081759428683074
6	1.3274584933725433	0.2325471413667303
7	0.7493001271499475	0.1531414132407609
8	0.7210783546687995	0.1684268205414941
9	0.49396497703631326	0.1298008305930959
>10	3.747101388815878	2.173408582168556
>50	0.4580483825080541	0.9255155242447957
>100	0.340435532114529	2.0491923770709644
>500	0.05603242889464757	1.1683927453795981
>1k	0.051323349476872296	2.5125859015738463
>5k	0.00216817836334501	0.45952079322676176
>10k+	0.0037450353548686356	86.33327471968086
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATGCGCTGTA	963374	5.542308909735017	No Hit
CTAACCTCCGCT	958718	5.515522853350138	No Hit
TTACTGTGCGAT	943701	5.429129767282328	No Hit
TCCGACACAATT	896202	5.155867118608497	No Hit
GCTGATGAGCTG	895075	5.149383466214648	No Hit
TACACGATCTAC	894703	5.14724334315297	No Hit
CCTCGTTCGACT	892051	5.131986336810036	No Hit
GCACACACGTTA	840025	4.832679771200134	No Hit
CACGCCATAATG	828512	4.766445263648779	No Hit
CAGGCGTATTGG	824114	4.741143486161516	No Hit
AGCTGTTGTTTG	809440	4.656723685604876	No Hit
TATCGTTGACCA	801009	4.608219982559147	No Hit
CCAGTGTATGCA	796205	4.580582479364784	No Hit
GACTTGGTATTC	770352	4.431849679597113	No Hit
AGGCTACACGAC	627715	3.6112563109179994	No Hit
TGAGTCACTGGT	623276	3.585718659652432	No Hit
ACACCTGGTGAT	585338	3.367460625475127	No Hit
AATCCGTACAGC	538711	3.099214609354478	No Hit
GGATCGCAGATC	513062	2.9516554254593412	No Hit
>>END_MODULE
>>Adapter Content	warn
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGCGA	130	3.6470738E-9	6.0000005	5
CGAACAG	55	0.009160845	6.0000005	5
TCACCGG	130	3.6470738E-9	6.0000005	5
CGTATGT	55	0.009160845	6.0000005	5
CGTATAG	205	0.0	6.0000005	5
AGTCACA	65	0.0012619963	6.0000005	3
ACGCATA	130	3.6470738E-9	6.0000005	2
GGGTATG	130	3.6470738E-9	6.0000005	4
GGCGTGT	55	0.009160845	6.0000005	3
CGCATGT	65	0.0012619963	6.0000005	5
GAATCGC	110	1.8154606E-7	6.0000005	1
GAATCCG	65	0.0012619963	6.0000005	1
ATTGTTG	110	1.8154606E-7	6.0000005	2
GGATCGA	110	1.8154606E-7	6.0000005	1
CCAGCGA	65	0.0012619963	6.0000005	1
TTGAGCA	110	1.8154606E-7	6.0000005	6
GGCTTAT	110	1.8154606E-7	6.0000005	3
GCCGATC	205	0.0	6.0000005	6
ACGATCC	65	0.0012619963	6.0000005	4
TCAGCGA	55	0.009160845	6.0000005	5
>>END_MODULE