FastQCFastQC Report
Mon 5 Oct 2015
000000000-AJ9MW l01n01.331000000351c8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJ9MW l01n01.331000000351c8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17382178
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCATGGGAGTGGGTTGCACCAGAAGTAGTTAGTCTAACCTTCGGGAG230930013.285446737457182No Hit
ACACCATGGGAGTGGGTTGCAAAAGAAGTAGGTAGCTTAACCTTCGGGAG10922396.283671700980165No Hit
ACACCATGGGAGTGGGTTGCACCAGAAGTAGCTAGCTTAACCTTCGGGAG7347284.226904131346486No Hit
ACACCATGGGAGTGGGTTGCACCAGAAGTAGTTAGTCTAACCTTAGGGGG4081302.347979637534491No Hit
GCTTGTAGTAACGAATGGTCTGGTGAACCTTCTGGACTGCTTCTGAATAA3122121.796161562722462No Hit
ACTTCTACCGATTCGAGTGGTTCGACGAATGACTAGGATTGATGGCTCAC3069521.7659006828718473No Hit
GCTATTACCGATTGAGTGTAAAGGTGAACCTTCTCGATAGACGCAAGTTT2696381.5512325325399383No Hit
GCTGCCCGGAACTGAGCCATTTCGAGAAAAATCGGGACTGACTGCTTGTG2512051.4451871336261775No Hit
ACACCATGGGAGTCGAACTCATTCGAAGCGGGGATGCTAAAATAGCTACC2448641.4087072402549325No Hit
GCTATCCGGGACTGAGCTGTTTCGAGAAGAGTGGGGACCGACTGTATGTG2427321.3964418037831623No Hit
GTTGTTTCCGATGGATGGCGAAATACAGGTTGTTGGACTGGCTTTCAGCC2252891.2960918936625778No Hit
GCTTTTACCGATTGAGTGTTCAGGTGAGTCTTCTTGATAGTTGCAAAGCT2009201.1558965740656897No Hit
GCTATCCGGGACTGAGCTGTTTCGAGAAGAGTGGGGACCGACGGCATGTG1991061.1454605976305157No Hit
GCTATCCGGGACTGAGCTGTTTCGAGAAGAGTGGGGACCGTCTGTCATGT1766061.0160176705128667No Hit
GCTACTACCGATCGAATGGCTTAGTGAGGCCTCTGGATCGGATCCGCCCC1758441.0116338700478158No Hit
GCTATTACCGATTGGGTGTATAGGTGAACCTTCTCGATAGTCGCAAGACT1679800.9663921287654516No Hit
GCTATTACCGATTGGGTGTATAGGTGAACCTTCCTGATAGTCGCAAGATT1635640.9409867969364943No Hit
GCTGTCCGGAACTGAGCTGTTTCGAGAAGACTGAGGACCGATGTGCGGAG1630090.9377938714009257No Hit
ACACCATGGGAGTGGGGGATACCAGAAGTGGGTAGGCTAACCGCAAGGAG1610300.9264086468335555No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGCTTTCGGATTGGACTCTCGTA1580810.9094429938526691No Hit
ACACCATGGGAGTTGAACTCATTCGAAGCGGGGATGCTAAAGTAGCTACC1545760.8892786623172309No Hit
GCTTGTAGTAACGAATGGTCTGGTGAACCTTCTGGACTGCTTCTGCATAA1513170.8705295734516123No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGCTTTCGGATTGGACTTTGGCA1463730.8420866475996276No Hit
GCTATCCGGGACTGAGCTGTTTCGAGAAGAGTGGGGACCGTCTTTCATAT1380870.7944171323064347No Hit
GCACCTACCGATTGAATGATTCGGTGAAACTTTCGGACCGTGGACAGGAC1161630.6682879441229977No Hit
GCTCCTACTGATTGAATTTTGTGATGAGGACCTTAGACTATAGACAAATG1015210.5840522401738147No Hit
ACACCATGGGAGTTTGTTGCACCAGAAGTAGGTAGTCTAACCGCAAGGAG973560.5600909161095923No Hit
GCTATCCGGGACTGAACTGATTCGAGAAGAGTGGGGACTGTCGCTTCGAG965970.5557243747014902No Hit
GCTTGTAGTAACGAATGGTCTGGTGAACCTTCTGGACTGCTTCTGCAAAG948310.5455645431775006No Hit
ACACCATGGGAGTCTATTGCACCAGAAGTAGGTAGCCTAACGAAAGAGGG937010.539063631726703No Hit
GCTCCTACCGATTTCGAGTGATGTGGTGAACTATCTGGACTGCGATTGGG896860.5159652605099315No Hit
GTTGTTTCCGATGGTTGGCGAAATACAGGTTGTTGGACTGGCTTCGTGCC812060.4671796595340354No Hit
GCTATTACCGATTGAGTGTATAGGTGAACCTTCCTGATAGCTTTCGGGCT751330.4322415752502362No Hit
ACACCATGGGAGTTGAACTCATTCGAAGCGGGGATGCTAAAATAGCTACC748970.4308838627702466No Hit
GCTTTTACCGATTGAGTGCTCAGGTGAACCTTCTCGATAGTGCAAACTAG699420.40237765370944883No Hit
ACACCATGGGAGCGGGTTCCGCCAGAAGTAGGTAGCCTAACCGCAAGGGG677940.39002016893395064No Hit
GCTATTACCGATTGGGTGTATAGGTGAACCTTCTCGATAGCCGCAAGGCT661170.3803723560994485No Hit
ACACCATGGGAGTGGGTTGCACCAGAAGTAGTTAGTCTAACCCTTCGGGG654810.37671343602625634No Hit
GCTGTCCGGAACTGAGCTGTTTCGAGAAGACTGAGGACTGATGCATTAGG651390.37474590353406806No Hit
GCTAGTACCGATTGAATGGCTTAGTGAGGCCTCAGGATCTGCTTAGAGAA603420.34714867147258527No Hit
ACACCATGGGAGTGGGTTGTACCAGAAGTAGTTAGTCTAACCCTTCGGGG560530.32247397305446995No Hit
GTTGTTTCCGATGGATGGCGAAATACAGGTTGTTGGACTGGCCTTACCGC559900.3221115328585405No Hit
GCTATTACCGATTGGGTGTATAGGTGAACCTTCCTGATAGATGCAAGTCT536410.308597691267458No Hit
GCTGTCCGGAACTGAGCTGTTTCGAGAAGACTGAGGATTAATGTATGGAG524170.30155599603225786No Hit
ACACCATGGGAGCGGGTTCTGCCAGAAGTGGGTAGCCTAACCGCAAGGAG487220.2802985908900484No Hit
GCTTGTAGTAACGAATGGTCTGGTGAACCTTCTGGACTGCTCAGCAATGA468630.2696037286006391No Hit
GCTCCTACCGATTGAATGGTCCGGTGAAGTGTTCGGATCGCGGCGACGGG458700.26389098075051354No Hit
GCTTGTAGTAACGAATGGTCTGGTGAACCTTCTGGACTGCGGTAGCAATA444260.255583621339052No Hit
GGTACTACCGATTGAATGGCCTGGTGAGGCCTTCGGATTGATCTTCATGG436730.25125159804484803No Hit
GCACCTACCGATTGAATGGTTCGGTGAAACTTTCGGACCGTGGACAGGAC435590.25059575388078525No Hit
GTTGTTTCCGATGGATGGCGAAATACAGGTTGTTGGACTGGCTCCGTGCC427650.246027856808278No Hit
ACTTCTACCGATTCGAGTGGTTCGACGAATGACTAGGATTGACGACTTTG415320.23893438440223086No Hit
ACACCATGGGAGTGGGTTGCACCAGAAGTAGCTAGTCTAACCTTCGGGAG404970.232980009754819No Hit
GCTTTTACCGATTGAGTGTTCAGGTGAGTCTTCTTGATAGTTTTTCTAAA389700.2241951497677679No Hit
GCTGCCTAGAACTGAGTTGATTCGAGAAGATGGGGGAGAGTAGTATATGT386300.22223912331354564No Hit
TTCTGCTTGGTAGGTGAACCTGCAGAAGGATCATGCTGACTGACTAGGCT383120.22040966327694952No Hit
GCTGTTACCGATTGAATGGTTCAATGAGTAGTAAGGAGTTTGAGGCAGCA364860.20990465061397945No Hit
GCTCCTACCGATACCGGGTGATGAGCTGAACCTTTTGGACCGAATTTCGG345820.19895090247033484No Hit
ACACCATGGGAGTGGGGGATACCAGAAGTAGGTAGGATAACCGCAAGGAG329730.1896942949266772No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGCTTTCGGATTGGATTTTGGCA324780.18684655053008892No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGTCTCCGGATTGGCTTTGGGGA316820.18226714741961567No Hit
ACGTCATGGGAGCCGAGAGCGCCCGAAGTCGTTTTAGCTAATAAAAGGCG316330.18198524948944833No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGCCTCTGGATTGGCTCCGACAC315750.18165157438843393No Hit
GCTATCCGGGACTGAGCTGTTTCGAGAAGAGTGGGGACCGACCGTGTGTG297620.17122135097224295No Hit
GCTACTACCGATTGAACGGCTTAGTGAGGTCTTCGGATCGACTTTGGGGA277320.1595427224367395No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGCCTTCGGATTGGGCCCGAAGA272750.1569135927615055No Hit
GTTGTTTCCGATGATGGCGTAATGCAGGTGATCGGACTAGCAGACGTTTT269470.15502660253507933No Hit
GTTGTTTCCGATGGATGGCGAAATACAGGTTGTTGGACTGGCTTTACCGC269180.15485976498457213No Hit
ACACCATGGGAGCGGGTTCTGCCAGAAGTAGTTAGCCTAACCGCAAGGAG259950.14954972846325704No Hit
ACACCATGGGAGTGGGTTGCACCAGAAGTAGTTAGTCTAACCTTCGGGGG258790.14888237826122824No Hit
GCTACCGGGGACTCTGAGCTGCGGCGAGAAGAATTTTTTTCTGATCGTCC253610.1459023144280308No Hit
GCTCCTACCGATTGAGTGATCCGGTGAACTTTCTGGACTTCTGGTTAACC250690.1442224328849929No Hit
GCTATCCGGGACTGAGTTGTTTCGAGAGGAGTGGAGACTGTTGTTTTGGG248680.14306607606940858No Hit
GCTGCTACCGATTGAATAGGAAATGTGAAATGGGAGGACGGGAGACTCGG241760.13908498693316798No Hit
GCACCTACCGATTGAATGATTCGGTGAAACTTTCGGACTGTGGTCTGGAC240440.1383255884274111No Hit
ACACCATGGGAATTTGTTGCACCAGAAGTAGGTAGTCTAACCGCAAGGAG240240.13821052804775097No Hit
ACACCATGGGAGTTGAATTCACCCGAAGCCGGAATACTAAACTAGTTACC232090.1335218175766006No Hit
ACACCATGGGAGTGGGTTGCACCAGAAGTAGTTAGTCTAACCCTCGGGAG231590.13323416662745025No Hit
GCTCCTACTGATTGAATTTTGTGATGAGGACCTTGGACTGTAGACGCATG222680.12810822671359137No Hit
GCTTGTAGTAACGAATGGTCTGGTGAACCTTCTGGACTGTGACAGCAATG209230.12037041618144745No Hit
GCTACTACCGATTGAATGGCTCAGTGAGGCCTTCGGACTGGCTCAGAGGG202660.11659068270961212No Hit
ACACCATGGGAGCGGGTTCTGCCAGAAGTAGTTAGCCTAACCGCAAGGGG201970.11619372439978465No Hit
GCACCTACCGATTGAATGATTCGGTGAAGATTCTGGACCGTGATCGATCG200770.11550336212182385No Hit
ACACCATGGGAGTGGGTTGCACCAGAAGTAGCTAGCCTAACCTTCGGGAG199470.11475546965403301No Hit
GTTGTTTCCGATGATGGCGTAATGCAGGTGATCGGACGAGCAGACGTTTT198530.11421468586963039No Hit
GCTGCTACTGATGTGCTTTTGGGTGAAATAATCGGATCATATCAACTTGG193280.1111943509035519No Hit
GCACCTACCGATTGAATGATTCGGTGAAGATTCTGGACCGTGGCCGATCG191870.11038317522694797No Hit
GCTATTACCGATTGAGTGTAAAGGTGAACCTTCTCGATAGTGTCACCGCT190860.1098021203096643No Hit
GCTACTACCGATTGAATGTTTTAGTGAGATCTTCGGCTTGGTTTTCCGAG189930.10926708954424467No Hit
GCACCATGGGAGTGGGTTGCACCAGAAGTAGTTAGTCTAACCTTCGGGAG186670.10739160535578453No Hit
GCCTCAATCGACTGGATGATCCGGTGAATGATTCGGACCGGAAATATGTT177910.10235196072667073No Hit
GCTACTACCGATTGAATGGCTCAGTGAGGCCTTCGGACTGGCTCAGGGGG174600.10044771144329555No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTACC1631400.094.97673
CCATGGG7021050.094.964144
CTACTAC1591300.094.96122
GCTATTA972050.094.96091
CTATCCG1036250.094.9312442
ACTACCG1633000.094.930184
CATGGGA7057700.094.928665
ACCATGG7008600.094.9132463
CACCATG7029550.094.9013442
CCGATTG4095900.094.884038
GCTATCC1034850.094.862291
CCGGAAC637350.094.850956
CGGAACT637500.094.828627
AGTAACG772400.094.8216557
TTGTAGT770250.094.8149953
ACACCAT6985900.094.7898941
CGATTGA3346750.094.774339
ACCGATT4707150.094.768917
CCGGGAC1048000.094.755246
CCGATTC425450.094.743228
Produced by FastQC (version 0.11.2)