##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AJ2K7 l01n03.3330000003458e.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 19366446 Sequences flagged as poor quality 0 Sequence length 251 %GC 57 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.0094204171483 32.0 32.0 32.0 18.0 33.0 2 31.42038441126472 32.0 32.0 33.0 29.0 33.0 3 30.020179386553423 32.0 32.0 33.0 16.0 33.0 4 29.75936457313851 32.0 30.0 33.0 16.0 33.0 5 30.330283780513987 32.0 32.0 32.0 29.0 33.0 6 34.394463238118135 37.0 35.0 37.0 30.0 37.0 7 34.08540348600874 37.0 34.0 37.0 19.0 37.0 8 31.78499689617806 33.0 32.0 37.0 16.0 37.0 9 34.41936336692855 37.0 33.0 37.0 32.0 37.0 10-14 35.23230827173969 37.4 34.6 37.4 32.4 37.4 15-19 34.231756141524365 37.0 34.4 38.0 22.0 38.0 20-24 34.28516575524492 37.0 34.6 38.0 22.8 38.0 25-29 34.89764146710243 37.4 34.8 38.4 28.0 38.6 30-34 34.42879804585725 37.2 34.4 37.8 26.0 38.6 35-39 34.485054821106566 37.0 34.8 38.4 22.6 38.4 40-44 33.10352049105963 37.0 32.2 38.4 16.4 39.0 45-49 35.30256614972102 37.8 35.2 38.8 26.8 39.0 50-54 33.79791080924192 37.0 33.6 38.4 18.8 39.0 55-59 35.284760311726785 37.0 35.0 38.0 29.4 38.6 60-64 35.90129555004568 38.0 36.0 38.4 30.2 38.4 65-69 32.57058074568767 36.0 29.4 37.6 18.2 38.4 70-74 34.71930721826813 37.2 34.0 38.0 26.4 38.4 75-79 35.05902727841753 38.2 35.2 38.8 25.6 39.0 80-84 35.518896115477254 38.0 35.8 38.8 26.8 39.0 85-89 35.72208728436802 37.8 36.4 38.6 29.0 38.6 90-94 34.80212423074424 37.6 35.4 38.4 22.4 38.4 95-99 36.06761730056201 38.0 36.4 38.6 30.2 39.0 100-104 34.791210178677076 37.0 35.0 38.0 27.8 38.6 105-109 31.249632193743757 35.4 27.6 38.0 14.4 38.6 110-114 34.12722603827258 36.8 32.0 38.4 27.4 38.8 115-119 32.58253126051109 36.8 31.0 38.4 15.8 39.0 120-124 35.955473172517046 38.2 36.2 39.0 28.6 39.0 125-129 32.75721607361516 36.6 31.4 38.6 18.0 39.0 130-134 33.71043570926746 36.6 32.8 38.6 20.2 39.0 135-139 30.54816196012423 35.6 22.6 37.8 15.6 38.6 140-144 30.53256370322154 34.8 27.0 37.6 14.6 38.6 145-149 30.373423125750595 33.8 25.4 37.8 15.4 38.6 150-154 29.694676658794286 33.8 20.2 37.8 13.4 38.8 155-159 30.15216396441557 34.2 24.0 37.8 13.6 38.8 160-164 28.07729325246357 31.4 19.4 36.2 13.6 38.4 165-169 24.817926335064264 23.6 16.4 33.0 13.6 36.8 170-174 29.464114014517683 33.4 21.0 37.2 13.6 38.2 175-179 25.30702257915572 27.0 15.4 34.0 12.6 37.2 180-184 32.15045987270974 36.4 28.0 37.6 16.6 38.2 185-189 27.865251745209214 31.0 16.8 36.2 12.6 37.8 190-194 29.023380531461477 33.0 20.8 37.0 13.8 38.0 195-199 26.90387860529495 29.2 16.6 36.8 13.0 37.2 200-204 28.18714534406571 31.0 20.6 35.2 14.8 37.0 205-209 25.805259312937437 28.2 15.2 34.2 12.2 36.8 210-214 25.651937583178658 27.0 15.4 33.4 12.2 36.4 215-219 26.41457576676691 28.0 16.2 35.6 13.0 37.0 220-224 24.74477961521696 26.4 17.6 32.6 12.2 36.0 225-229 23.414761211220686 22.8 14.0 30.0 14.0 35.4 230-234 24.359615667221544 24.6 15.0 33.4 12.6 35.8 235-239 22.659708487556262 23.0 12.8 30.8 12.0 34.6 240-244 22.185000686238457 22.0 13.0 31.2 12.0 35.2 245-249 26.619568815052595 29.0 18.0 34.6 12.6 37.0 250-251 21.119032939755698 19.5 13.0 24.5 12.0 31.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -5.360882944857149 1101 2 -1.3191044148080522 1101 3 -0.42431117373666183 1101 4 0.012819488267425072 1101 5 0.40023109861734696 1101 6 0.3730384080062663 1101 7 0.1650424624351743 1101 8 0.7145640035166672 1101 9 0.27318400307110124 1101 10-14 0.2995043206919945 1101 15-19 0.7021337351583128 1101 20-24 0.5591654226741838 1101 25-29 0.446390789676272 1101 30-34 0.4181400350856421 1101 35-39 0.43431776668474953 1101 40-44 0.5091550006835277 1101 45-49 0.5086442070763013 1101 50-54 0.36664955703418656 1101 55-59 0.20366267198947696 1101 60-64 0.2811398714090316 1101 65-69 0.4019496308962829 1101 70-74 0.22062957908999437 1101 75-79 0.24028619557005726 1101 80-84 0.25846503246994956 1101 85-89 0.34605195274882306 1101 90-94 0.5861862478282589 1101 95-99 0.2947169060183441 1101 100-104 0.3783350996426975 1101 105-109 0.5897684742926543 1101 110-114 0.16170125524254075 1101 115-119 0.4760764396404724 1101 120-124 0.2782233179636151 1101 125-129 0.40989162207915086 1101 130-134 0.4480590565361595 1101 135-139 0.4926939874400347 1101 140-144 0.43526032815957194 1101 145-149 0.7175510075100426 1101 150-154 0.7068825824073599 1101 155-159 0.47666352863799943 1101 160-164 0.7423295329962869 1101 165-169 0.768676910053518 1101 170-174 0.6262389732611773 1101 175-179 0.9410085231170271 1101 180-184 0.46292616204016923 1101 185-189 0.3539211017023156 1101 190-194 0.26454806221314087 1101 195-199 0.8395976626454882 1101 200-204 0.3349235502759065 1101 205-209 0.6705048380423619 1101 210-214 0.28490777456631733 1101 215-219 0.5923361525171451 1101 220-224 -0.10629484983539683 1101 225-229 -0.015037505951742247 1101 230-234 0.6054641080028169 1101 235-239 0.25815747070688744 1101 240-244 0.5506836820678274 1101 245-249 0.15799450401667414 1101 250-251 0.12087879597039475 1102 1 0.5411914603740904 1102 2 0.1463457699715498 1102 3 0.31537763063821345 1102 4 0.3626642648603884 1102 5 0.4092201610583537 1102 6 0.22501307022875494 1102 7 0.2238092705485215 1102 8 0.49455068602562946 1102 9 0.22165105214175895 1102 10-14 0.24965630047917386 1102 15-19 0.3097002058855125 1102 20-24 -0.09691992931958993 1102 25-29 0.22103353877506748 1102 30-34 0.12998026100717652 1102 35-39 0.1764480315249486 1102 40-44 0.05720933391618388 1102 45-49 0.25957809874717697 1102 50-54 0.20453518486856126 1102 55-59 -0.14139516578958933 1102 60-64 0.19946933586165727 1102 65-69 -0.13126492092048636 1102 70-74 0.025323487889231444 1102 75-79 0.17220869596273758 1102 80-84 0.21252008589448224 1102 85-89 0.03658648898149153 1102 90-94 0.1541399129718215 1102 95-99 0.10288783539686364 1102 100-104 0.1365536253532582 1102 105-109 0.14661541460855787 1102 110-114 0.1113806804462456 1102 115-119 0.013033527395400313 1102 120-124 0.14513998795055016 1102 125-129 0.2889805608727798 1102 130-134 0.2616502492669639 1102 135-139 -0.025619872788524845 1102 140-144 0.3021128432707627 1102 145-149 0.3929412780204231 1102 150-154 0.6748716401857493 1102 155-159 0.5969772482278763 1102 160-164 0.5435650516824815 1102 165-169 -0.3628643171637336 1102 170-174 0.808927678500833 1102 175-179 1.0292985001247246 1102 180-184 0.696133067922581 1102 185-189 0.8509791095727337 1102 190-194 0.5147949966158443 1102 195-199 1.0648820026768853 1102 200-204 0.6248806316562003 1102 205-209 1.1408627254317025 1102 210-214 0.7151966866718276 1102 215-219 0.8338833813667357 1102 220-224 0.23533310026855503 1102 225-229 0.7112729701049645 1102 230-234 1.3101455725974525 1102 235-239 0.5092497142144872 1102 240-244 0.95692301434012 1102 245-249 0.33933640083148475 1102 250-251 0.6651485974917328 1103 1 0.5190926302702294 1103 2 0.153700039805738 1103 3 0.27279240842513985 1103 4 0.4380515091255326 1103 5 0.13386620128155613 1103 6 0.25805774170247986 1103 7 0.27011344582935237 1103 8 0.22506781076893034 1103 9 0.23673411025840352 1103 10-14 0.28299014068904427 1103 15-19 -0.7387688657225979 1103 20-24 -0.6206174440900085 1103 25-29 -0.00642294052779846 1103 30-34 -0.027682243832195752 1103 35-39 0.15632671133464981 1103 40-44 -0.023562132030825467 1103 45-49 0.2920290510257928 1103 50-54 0.2203863486180282 1103 55-59 -0.11940523334870079 1103 60-64 0.20211498674881057 1103 65-69 -0.6547510222587896 1103 70-74 -0.21494485386249806 1103 75-79 0.1751354439504098 1103 80-84 0.172194605469997 1103 85-89 0.289379580095634 1103 90-94 0.2890122188163886 1103 95-99 0.15825712653082036 1103 100-104 0.023120245455864108 1103 105-109 0.3810919636646979 1103 110-114 0.019769813731222996 1103 115-119 -0.03883623426600735 1103 120-124 0.19255815089994854 1103 125-129 0.274681370446892 1103 130-134 0.4008670719419527 1103 135-139 0.11510710499668164 1103 140-144 0.11827515013598244 1103 145-149 -1.1785137813707607 1103 150-154 0.5544456879076662 1103 155-159 0.6769725055010838 1103 160-164 0.46262564956408525 1103 165-169 -1.5718343090055527 1103 170-174 0.3452606376362546 1103 175-179 0.23141355741192982 1103 180-184 0.6164667583000707 1103 185-189 0.7242299003473818 1103 190-194 0.441909058759002 1103 195-199 0.6965791754325501 1103 200-204 0.6240846854500859 1103 205-209 0.9602044726027508 1103 210-214 0.7312205973407053 1103 215-219 1.012715231115397 1103 220-224 0.42847939846812366 1103 225-229 0.3178117010166197 1103 230-234 1.101815719236459 1103 235-239 0.9502588322095562 1103 240-244 1.2580603940618644 1103 245-249 0.4458481681779176 1103 250-251 0.16356255818414667 2112 1 0.36313083304480287 2112 2 0.05657818581083163 2112 3 0.010987875175970885 2112 4 -0.030450465821914463 2112 5 0.03571824391439904 2112 6 -0.21738938377105654 2112 7 -0.32559029031762066 2112 8 0.03426211366060272 2112 9 -0.10631010969951404 2112 10-14 0.04971554699380931 2112 15-19 -0.5611336912628317 2112 20-24 -0.8479042999117894 2112 25-29 -0.0890804806701837 2112 30-34 -0.1886647410754847 2112 35-39 -0.32744735850083373 2112 40-44 -0.2819085646409647 2112 45-49 -0.008693719727048688 2112 50-54 0.12547422370737848 2112 55-59 -0.40434936947468714 2112 60-64 -0.06800528431312358 2112 65-69 -0.588509318937593 2112 70-74 -0.4982823471449649 2112 75-79 -0.01848635831667167 2112 80-84 -0.1669140585879063 2112 85-89 0.01647963815617004 2112 90-94 -0.5764975246335524 2112 95-99 -0.3318160289442318 2112 100-104 -0.577861570406121 2112 105-109 -0.21939018710391522 2112 110-114 -0.9146802536143923 2112 115-119 -0.7566884634115105 2112 120-124 -0.1618295752037966 2112 125-129 0.07275403474115194 2112 130-134 0.08003060573991405 2112 135-139 -0.32818564091833125 2112 140-144 -0.4871269919772274 2112 145-149 -2.1747278000469876 2112 150-154 0.1736020026791394 2112 155-159 -0.10783116541938753 2112 160-164 -0.6135720219596799 2112 165-169 -1.329522912852017 2112 170-174 -0.5044873519535997 2112 175-179 -0.10290687370813956 2112 180-184 -0.017831067177127125 2112 185-189 0.3438028008722256 2112 190-194 0.15243115335736235 2112 195-199 0.4488398929485591 2112 200-204 -0.033222442156564114 2112 205-209 0.535271937454656 2112 210-214 0.06786170871797026 2112 215-219 0.023723226244932505 2112 220-224 -0.29471292295000495 2112 225-229 -0.20181592673881 2112 230-234 0.5708292301797648 2112 235-239 0.27701548271754817 2112 240-244 0.9351492347990664 2112 245-249 0.14549297758012258 2112 250-251 -0.0842498060493746 1113 1 0.3946611982863111 1113 2 0.15663570174895725 1113 3 0.16248924820945732 1113 4 0.303953215231612 1113 5 0.08514888674538312 1113 6 -0.08926180658939842 1113 7 -0.3626232436355039 1113 8 0.42858363632123186 1113 9 0.03619183005764626 1113 10-14 0.20843148216680873 1113 15-19 0.044274307141897395 1113 20-24 0.03719001235981523 1113 25-29 0.17595028710579896 1113 30-34 -0.06571342944360481 1113 35-39 -0.042829639374126316 1113 40-44 -0.07787922247599255 1113 45-49 0.24234048004947084 1113 50-54 0.2642258032437468 1113 55-59 -0.3844757864930699 1113 60-64 0.1913956792589815 1113 65-69 -0.3027367352250607 1113 70-74 -0.3230394216948511 1113 75-79 0.04380102428978461 1113 80-84 0.05413964052131348 1113 85-89 0.0823497231382504 1113 90-94 -0.1261947888049164 1113 95-99 -0.24333331642329625 1113 100-104 -0.4176492630928621 1113 105-109 -0.11016274115963398 1113 110-114 -0.9187924893867958 1113 115-119 -0.2775394166713028 1113 120-124 -0.03436361146140854 1113 125-129 0.19384871102936785 1113 130-134 0.23928161375098256 1113 135-139 -0.12224356764635047 1113 140-144 -0.09988655603743979 1113 145-149 -0.7498081556122997 1113 150-154 0.4765792746657205 1113 155-159 0.12079557458018897 1113 160-164 -0.27993985515150754 1113 165-169 -0.7831306845468227 1113 170-174 0.10899344923909737 1113 175-179 -0.22495947694061513 1113 180-184 0.3119126960692782 1113 185-189 0.43350282691239883 1113 190-194 0.5478218293317703 1113 195-199 0.7252094694858755 1113 200-204 0.3054833942454991 1113 205-209 0.6216393821435275 1113 210-214 0.4057696037748215 1113 215-219 -0.3176206259460166 1113 220-224 0.1060819762666867 1113 225-229 0.05103762523052424 1113 230-234 0.5622701612040366 1113 235-239 0.29356146357109125 1113 240-244 0.8831524774165942 1113 245-249 0.7957113631506267 1113 250-251 0.5958922727995493 2113 1 0.34680797454143786 2113 2 0.0958337765291688 2113 3 0.1142977751976666 2113 4 0.19014537972426027 2113 5 0.16476393083593877 2113 6 -0.1061093179838295 2113 7 -0.057546691200712985 2113 8 0.15835310966804172 2113 9 0.04605576418133239 2113 10-14 0.11966342653942519 2113 15-19 0.33072671455508384 2113 20-24 0.056343886343945826 2113 25-29 0.12366913277251257 2113 30-34 0.15049071800248726 2113 35-39 0.02756656483189346 2113 40-44 -0.20940102349786827 2113 45-49 0.207368914302954 2113 50-54 0.16200543621943808 2113 55-59 -0.14434571182782463 2113 60-64 0.1582344728925591 2113 65-69 -0.35770708319669353 2113 70-74 -0.20308270104982284 2113 75-79 -0.028667874158593065 2113 80-84 0.011094021812048993 2113 85-89 0.10660564103182679 2113 90-94 -0.11481742256184901 2113 95-99 -0.18889190389892008 2113 100-104 -0.25486955621082785 2113 105-109 -0.13928949045817518 2113 110-114 -1.1583797360247559 2113 115-119 -0.4160721631357305 2113 120-124 -0.1508636571985491 2113 125-129 0.18578859981683848 2113 130-134 0.29783777231172337 2113 135-139 -0.050643422274674776 2113 140-144 -0.14923065707522198 2113 145-149 -0.6506504733132878 2113 150-154 0.27453956228956145 2113 155-159 -0.26386065003246983 2113 160-164 -0.7600028297944768 2113 165-169 -0.4615742116729109 2113 170-174 0.47658712102967726 2113 175-179 0.568084575020837 2113 180-184 0.3115931391850637 2113 185-189 0.5606483916379048 2113 190-194 0.617741498516537 2113 195-199 0.9441020048458562 2113 200-204 0.3601291565971536 2113 205-209 0.7062559050120427 2113 210-214 0.5868137966255453 2113 215-219 0.40753425308729874 2113 220-224 0.45645461336525983 2113 225-229 0.29874937361723397 2113 230-234 0.5909593319011783 2113 235-239 0.6455949986186518 2113 240-244 1.2346238454661034 2113 245-249 0.5745667259687544 2113 250-251 0.5254723763104536 1112 1 0.3952483191474947 1112 2 0.10300458469674467 1112 3 0.20626940863360232 1112 4 0.12855422368723168 1112 5 0.17220994704856807 1112 6 -0.051269064626907834 1112 7 0.10057535044774824 1112 8 0.14652126715443003 1112 9 0.0982795529453071 1112 10-14 0.11287518536349239 1112 15-19 0.4531446628200655 1112 20-24 -0.21678435160220744 1112 25-29 0.03979982110642055 1112 30-34 -0.12095540766733848 1112 35-39 -0.09584321203452362 1112 40-44 0.04283401400368092 1112 45-49 0.12091752999204175 1112 50-54 -0.05529707549052887 1112 55-59 -0.26724165940796496 1112 60-64 0.0034753389486184005 1112 65-69 -0.5173927758671297 1112 70-74 -0.3087889826386956 1112 75-79 -0.053320752050957765 1112 80-84 -0.07452181614379327 1112 85-89 -0.07561975833748846 1112 90-94 -0.2743970116488441 1112 95-99 0.07763868252898476 1112 100-104 -0.19609856994806307 1112 105-109 -0.4185988060903334 1112 110-114 -0.3575773702366334 1112 115-119 -0.48888851512746356 1112 120-124 -0.10256573851670936 1112 125-129 -0.20488773271765126 1112 130-134 -0.06435860172673102 1112 135-139 -0.11617805530156033 1112 140-144 -0.3455614078382361 1112 145-149 -0.680028160520834 1112 150-154 -0.04074478468412934 1112 155-159 0.7336566454620339 1112 160-164 0.48144563646726013 1112 165-169 -0.6292502651339937 1112 170-174 -0.08282451447104222 1112 175-179 0.06228667942137989 1112 180-184 -0.02103824423340228 1112 185-189 0.1606783260656286 1112 190-194 -0.020800702066551935 1112 195-199 0.24050922872167035 1112 200-204 0.3174869179125359 1112 205-209 0.06354359063399428 1112 210-214 -0.10272152011301472 1112 215-219 -0.030880240071020637 1112 220-224 0.020786200544726796 1112 225-229 -0.4876472671258085 1112 230-234 0.00137340520704754 1112 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0.2773645980252084 2110 3 0.3002455003983364 2110 4 0.22228383035522015 2110 5 0.05612147472326967 2110 6 -0.003887365579188895 2110 7 0.1302179277077613 2110 8 -0.9531593865799834 2110 9 -0.22464132223987576 2110 10-14 0.21751868084572834 2110 15-19 0.36121995240028326 2110 20-24 -0.30741302520399927 2110 25-29 -0.32932129596043325 2110 30-34 0.16533324649192593 2110 35-39 0.2698320976515234 2110 40-44 0.3277210193409914 2110 45-49 0.189908201313834 2110 50-54 -0.4624679046935185 2110 55-59 0.19070990962593726 2110 60-64 -0.22974140256228992 2110 65-69 0.5006110098170353 2110 70-74 0.1505864995230226 2110 75-79 -0.18728119636345752 2110 80-84 -0.022650957667785576 2110 85-89 -0.19017158887484698 2110 90-94 0.07158985122540429 2110 95-99 -0.30909804324507206 2110 100-104 0.22316531501095938 2110 105-109 0.18230394215663281 2110 110-114 0.28478039887704654 2110 115-119 0.4298074436430497 2110 120-124 -0.09842692719441004 2110 125-129 -0.07516814745211065 2110 130-134 -0.3270589806862745 2110 135-139 -0.4834284684585697 2110 140-144 -0.22892177785251633 2110 145-149 0.3039525602278488 2110 150-154 -0.10531056386757243 2110 155-159 -0.17484978024038256 2110 160-164 0.36993613643221224 2110 165-169 0.5177132484526226 2110 170-174 -0.1372109318667576 2110 175-179 -2.433546169260236 2110 180-184 -1.7007941128735595 2110 185-189 -0.3208017215597927 2110 190-194 -0.2439084938700482 2110 195-199 -0.4711087139445844 2110 200-204 -0.2598314720232402 2110 205-209 -0.06323380911957699 2110 210-214 -0.20264370241219964 2110 215-219 -0.6833710356722804 2110 220-224 0.07933223089326447 2110 225-229 -0.5182671398126821 2110 230-234 -0.24178523800591023 2110 235-239 -0.030780180687457914 2110 240-244 -0.811828246531487 2110 245-249 -0.06582789238118991 2110 250-251 -0.443663962174238 2109 1 0.7754799606874059 2109 2 0.3285483857502953 2109 3 -0.04249515344859489 2109 4 0.039774791266015797 2109 5 -0.2600627552062491 2109 6 -0.06977543203880288 2109 7 -0.08205033134976247 2109 8 0.14646019930815868 2109 9 -0.05584210584171956 2109 10-14 0.2959653276890464 2109 15-19 0.3157261044861954 2109 20-24 -0.07101896862692314 2109 25-29 -0.0912803911469311 2109 30-34 0.25738138844376834 2109 35-39 -0.06395031633377357 2109 40-44 0.240485207314741 2109 45-49 -0.524097023351878 2109 50-54 -0.20302127054234376 2109 55-59 0.22878006111096738 2109 60-64 -0.0806288366962633 2109 65-69 0.4725025672288439 2109 70-74 0.4490232453451952 2109 75-79 0.012708839786135684 2109 80-84 -0.045430965326424655 2109 85-89 -0.0754900102597702 2109 90-94 0.18887280336068102 2109 95-99 -0.2618784771861229 2109 100-104 0.0876359905118207 2109 105-109 0.04280561509113312 2109 110-114 0.4835803866890842 2109 115-119 -0.15299085460266326 2109 120-124 -0.1819757310417316 2109 125-129 -0.1926421298984735 2109 130-134 -0.3368809414807288 2109 135-139 0.13682362142783688 2109 140-144 0.3929510374637992 2109 145-149 0.6707865824553316 2109 150-154 -0.4778053778314977 2109 155-159 -0.04252940841891828 2109 160-164 0.21864649674193615 2109 165-169 0.5071641897839321 2109 170-174 0.3011511744881723 2109 175-179 -2.103031700427003 2109 180-184 -1.0635082563630789 2109 185-189 -0.07116126216409313 2109 190-194 -0.01169085309572182 2109 195-199 0.12652524622557948 2109 200-204 -0.19036914170703767 2109 205-209 -0.273891400311026 2109 210-214 -0.2181165952467765 2109 215-219 0.18020422860248075 2109 220-224 0.5081797962278429 2109 225-229 0.3507464804391738 2109 230-234 0.10913286222537977 2109 235-239 0.32671865875219197 2109 240-244 -0.5921074517278768 2109 245-249 -0.37628493238177185 2109 250-251 -0.3129132684048308 2108 1 0.8311649618108348 2108 2 0.20874342579459437 2108 3 0.09688936016399552 2108 4 0.04417122731705092 2108 5 -0.11988198678562156 2108 6 -0.01046163299215408 2108 7 -0.022115934363732492 2108 8 -0.06496641426667438 2108 9 -0.0058584821860776515 2108 10-14 0.24153788977317703 2108 15-19 -0.06509979195926263 2108 20-24 0.45482799937580154 2108 25-29 -0.08684112735985394 2108 30-34 0.23998132717797915 2108 35-39 0.16494803648928524 2108 40-44 0.07234603239093218 2108 45-49 -0.09362491145529361 2108 50-54 -0.25367693423049076 2108 55-59 0.20031057556006715 2108 60-64 -0.17815093284801264 2108 65-69 0.40970203443929876 2108 70-74 0.24808469237132158 2108 75-79 -0.08504549713813958 2108 80-84 0.07038357888399815 2108 85-89 -0.09391580230307284 2108 90-94 0.31736772263398194 2108 95-99 -0.25838213435415724 2108 100-104 0.10216054305107036 2108 105-109 0.31506740179009185 2108 110-114 0.2749985167709852 2108 115-119 0.3501496041426435 2108 120-124 -0.04251814190740788 2108 125-129 0.1450544496048849 2108 130-134 0.03861972850324946 2108 135-139 0.6334721429706036 2108 140-144 0.15448971009887202 2108 145-149 0.3479180006561293 2108 150-154 -0.3521007623396315 2108 155-159 -1.013802386500025 2108 160-164 0.04084248541384028 2108 165-169 0.5070286569864102 2108 170-174 0.30823401368120784 2108 175-179 -1.7025431817763312 2108 180-184 -1.0758336437805909 2108 185-189 0.10843165238446772 2108 190-194 -0.13739630347139098 2108 195-199 -0.10038533288080131 2108 200-204 -0.1455647949988652 2108 205-209 -0.1400475620750825 2108 210-214 -0.04189259610835805 2108 215-219 -0.1815198422466331 2108 220-224 0.224156426314714 2108 225-229 -0.5132555464221973 2108 230-234 -0.21235846916853873 2108 235-239 0.021904790863061407 2108 240-244 -0.3579572371342685 2108 245-249 -0.11840903228299382 2108 250-251 -0.23713430967615423 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 8.0 13 390.0 14 1357.0 15 1183.0 16 5247.0 17 27899.0 18 63429.0 19 88642.0 20 122425.0 21 165683.0 22 221578.0 23 299337.0 24 424401.0 25 601864.0 26 834337.0 27 1107332.0 28 1396086.0 29 1642621.0 30 1832114.0 31 1985867.0 32 2100005.0 33 2094644.0 34 1927436.0 35 1542652.0 36 797413.0 37 82486.0 38 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 1.2603190074213928 1.8905017471971886 1.8828286821443645 94.96635056323706 2 0.9584825712721153 1.497765852225331 1.191116903489515 96.35263467301304 3 2.4803621686704935 1.2906498177311418 66.46022713718355 29.768760876414806 4 94.86771062009574 1.7627034801689212 2.1675841900772186 1.2020017096581226 5 1.52579363296704 2.0881373897926343 95.15978305983452 1.2262859174058058 6 0.9833244571564653 1.4292555278340693 96.26564419718517 1.3217758178242924 7 1.1231229519344954 1.2624825432606477 78.68906354836608 18.925330956438778 8 95.59194288926321 1.352927635767554 1.931505656742595 1.123623818226638 9 0.978470701335702 4.710203410579308 1.1921082474296008 93.11921764065539 10-14 1.0324754474827236 2.20262509703639 20.329534907953683 76.4353645475272 15-19 22.49147726950004 17.222429969856112 26.335732431237 33.950360329406855 20-24 20.83008871798344 1.8154492944229268 42.413443177203355 34.941018810390275 25-29 17.52142236112914 7.6715273416712595 25.664891741107276 49.142158556092326 30-34 38.70118761077794 20.483050942852397 22.706591596620257 18.109169849749406 35-39 23.674609166803243 34.93329958423967 18.862115434086355 22.52997581487073 40-44 31.661539757991736 14.031155742256479 26.384221451886425 27.923083047865365 45-49 26.760187181478727 25.242762662803493 6.557201047626394 41.43984910809139 50-54 28.701312569172476 20.812682925922495 14.796899751250178 35.68910475365485 55-59 19.83064626312954 1.403300326761038 40.69040958779944 38.07564382230999 60-64 20.202332425887537 19.461700923339265 3.0739145427095917 57.262052108063614 65-69 37.9469356432254 4.676822995814513 31.759213848529566 25.617027512430518 70-74 18.38294439774856 2.492939592530297 44.09593995718161 35.028176052539536 75-79 18.943544933334696 10.385907667312836 27.698576186874966 42.9719712124775 80-84 1.6667973049882254 35.06374995184971 39.110507937284936 24.158944805877134 85-89 22.711364800748676 21.779859866905884 20.16161767626337 35.347157656082075 90-94 1.1436367829182494 19.69688604713534 56.84343942094486 22.316037749001545 95-99 15.294782532634022 21.029401057891572 6.955610750676712 56.720205658797695 100-104 0.9749408848686022 37.40639351174707 21.11583715463333 40.502828448750996 105-109 40.60352529317976 26.107852726308174 10.620067306102523 22.668554674409545 110-114 2.7858740834534124 18.119403012819184 40.13715268149871 38.957570222228696 115-119 13.246054541963973 23.44787783984733 26.196559761145643 37.10950785704305 120-124 1.1632253021540453 1.8372415878473518 35.16402751439268 61.83550559560592 125-129 21.879406267933724 15.890823747423767 15.760620198460781 46.46914978618173 130-134 4.444598663069104 22.417550437493798 30.106587445109962 43.03126345432714 135-139 22.660488145321036 30.26631009117522 13.764721725400728 33.30848003810301 140-144 34.53113596578329 24.33044039159276 11.898246069516318 29.240177573107633 145-149 9.183443363846933 18.76673293592433 44.154964726104104 27.894858974124624 150-154 34.00848250628949 25.33394924396557 11.000357009231328 29.65721124051362 155-159 12.971997030327609 18.98485968979543 36.01321791308534 32.029925366791616 160-164 41.55733108240233 16.21988997072452 6.789937676638632 35.43284127023452 165-169 31.11967575258775 25.408892266552158 38.325917930424616 5.14551405043548 170-174 29.179848486397557 7.494707082548858 9.851361473344154 53.474082957709435 175-179 31.84336041832353 16.383053452347426 39.913206584212716 11.86037954511633 180-184 5.178319243499814 29.21025468482963 21.56397720056638 44.04744887110418 185-189 23.05755738559362 25.789308993503507 17.670998592100997 33.48213502880188 190-194 10.619326850161356 19.887653108887402 37.09989638780394 32.393123653147306 195-199 26.24110071615618 28.682033864138006 10.393728410468292 34.68313700923753 200-204 16.067531440719687 5.407493971790178 19.55310024358625 58.971874343903885 205-209 31.15539526457255 26.384446583539383 6.005841236951787 36.45431691493627 210-214 9.597645329452806 2.319326943105617 39.40419114586125 48.67883658158033 215-219 18.894539555683064 21.172232633700578 21.87590330203074 38.05732450858562 220-224 15.341017138611804 23.684404459135145 19.508296979218592 41.46628142303446 225-229 6.61093315727625 35.12482155992896 35.24263873712296 23.021606545671826 230-234 25.283193416076443 26.396611954511428 9.801774677708032 38.5184199517041 235-239 18.396886036808198 35.94092999820411 3.4271130593605044 42.23507090562719 240-244 21.22147140471721 12.055083312653235 30.495699623978506 36.22774565865105 245-249 2.279197742321952 9.751294584458089 21.182955303208445 66.7865523700115 250-251 34.21728576883146 2.667203436996221 12.297501882831313 50.818008911341 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 14.5 1 3.666666666666667 2 1.9999999999999998 3 2.6666666666666665 4 3.3333333333333335 5 0.6666666666666666 6 1.0 7 1.0 8 0.0 9 1.9999999999999998 10 1.6666666666666665 11 0.6666666666666666 12 1.3333333333333333 13 1.3333333333333333 14 2.6666666666666665 15 1.6666666666666665 16 1.0 17 1.0 18 2.333333333333333 19 5.999999999999998 20 7.33333333333333 21 4.0 22 2.333333333333333 23 4.666666666666666 24 4.333333333333333 25 7.666666666666663 26 10.333333333333334 27 19.666666666666664 28 35.66666666666664 29 111.66666666666616 30 238.0000000000024 31 346.6666666666644 32 404.99999999999443 33 511.99999999998835 34 472.99999999999056 35 694.000000000009 36 1380.666666666632 37 2424.333333333318 38 3770.0000000005966 39 8799.333333331457 40 19710.33333333315 41 27958.99999996981 42 34717.33333329983 43 44763.00000003959 44 62442.00000016822 45 74022.33333340057 46 92763.66666646118 47 99388.33333303145 48 86694.6666665495 49 92980.99999979135 50 148440.99999974255 51 183997.3333341107 52 270578.6666692274 53 416507.66666073323 54 623490.3333326749 55 870379.3333614166 56 1049880.666715191 57 1176265.3333524314 58 1148369.0000255932 59 993031.0000423618 60 783380.0000179552 61 530911.6666552307 62 323948.33333278756 63 192355.0000010206 64 110652.33333286754 65 65292.00000018896 66 41803.33333335139 67 32261.33333328749 68 24069.666666650624 69 12330.33333333788 70 7322.99999999781 71 4396.333333333805 72 2593.333333333395 73 1606.9999999999138 74 1020.3333333333794 75 650.000000000004 76 424.66666666666 77 301.00000000000034 78 231.0000000000022 79 188.66666666666765 80 159.00000000000014 81 127.3333333333326 82 107.66666666666622 83 107.99999999999955 84 98.99999999999967 85 98.99999999999967 86 98.33333333333302 87 95.33333333333306 88 88.99999999999982 89 96.9999999999997 90 87.33333333333317 91 81.33333333333326 92 77.99999999999997 93 75.66666666666667 94 81.33333333333326 95 83.99999999999989 96 88.33333333333316 97 99.99999999999966 98 130.33333333333266 99 257.66666666666947 100 894.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 5.16357002208872E-6 3 0.0 4 5.16357002208872E-6 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 2.065428008835488E-6 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-154 0.0 155-159 0.0 160-164 1.032714004417744E-6 165-169 0.0 170-174 0.0 175-179 0.0 180-184 0.0 185-189 0.0 190-194 0.0 195-199 0.0 200-204 0.0 205-209 0.0 210-214 0.0 215-219 0.0 220-224 0.0 225-229 0.0 230-234 0.0 235-239 0.0 240-244 0.0 245-249 0.0 250-251 2.58178501104436E-6 >>END_MODULE >>Sequence Length Distribution pass #Length Count 251 1.9366446E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 25.58860991245752 #Duplication Level Percentage of deduplicated Percentage of total 1 86.80369248326562 22.21185825915205 2 5.951908064117212 3.0460210737501106 3 2.2349888161993294 1.7157077092928956 4 1.161911786682615 1.1892682984843197 5 0.6570006164953015 0.8405866243871185 6 0.5291081574425142 0.8123485345377391 7 0.4138693103315112 0.7413238235767605 8 0.24730525175315116 0.5062558093130789 9 0.1858278307444103 0.42795682846172056 >10 1.4546392241381316 7.282651583219869 >50 0.19250162166471385 3.48648498177473 >100 0.13288522012747472 6.67009615293482 >500 0.013617553463695774 2.412923609551298 >1k 0.014007919189383121 7.7346443147541 >5k 0.0026091285207429423 4.602250077250306 >10k+ 0.004127015864166576 36.31962231955915 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCCGTTTGCTCCCCTGGCTTTCGCGCCTCAGCGTCAATACCGGTCCAGGA 304128 1.5703862236777981 No Hit CCTGTTCGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTACCGTCCCAGAG 296276 1.5298418718643576 No Hit CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGACCAGTG 204546 1.0561875937381593 No Hit CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTGGCCCAGAA 197933 1.0220409051820865 No Hit CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTAACGTCCCAGCG 175135 0.9043218358185079 No Hit CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGGCCAGTG 172086 0.8885781108211595 No Hit CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGCAGGCCCAGGG 123525 0.6378299869785091 No Hit CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTAGCCCAGGG 103630 0.535100761389054 No Hit CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTATCGGCCCAGAG 103515 0.5345069508365138 No Hit CCTGTTCGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTACCGTTCCAGAG 99022 0.5113070307272692 No Hit CCCGTTTGCTCCCCTGGCTTTCGCGCCTCAGCGTCAATGCCGGTCCAGGA 98659 0.509432654809251 No Hit CCCGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTGTCGGTCCAGGA 96947 0.5005926229314351 No Hit CCTGTTTGCTCCCCACGCTTTCGCACATGAGTGTCAGTATTGGGCCAGGA 93913 0.484926351484418 No Hit CCCGTTCGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTGTCAGCCCAGCA 91619 0.4730811218537464 No Hit CCCGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAAGAACAGGCCAGGT 89794 0.4636576065634345 No Hit CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTCAGCCCAGAG 80885 0.4176553612366461 No Hit CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTATTGGTCCAGGA 76848 0.3968100290574739 No Hit CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTATCCAACCAGGG 72704 0.3754121948859383 No Hit CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACCAGTCCAGTG 70699 0.3650592369916504 No Hit CCTGTTTGCTCCCCACGCTTTCGTGCCTGAACGTCGGTACTGGTCCAGGA 64872 0.3349711144729394 No Hit CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCGGGCCAGTG 63781 0.32933765957884065 No Hit CCTGTTTGCTCCCCACGCTGTCGCACCTGAGTGTCAGTATTGGGCCAGGT 61659 0.31838056399196835 No Hit CCCGTTTGCTCCCCTGGCTTTCGCTCCTCAGCGTCAGTGTTGGTCCAGGA 61097 0.3154786376395545 No Hit CCTGTTTGCTCCCCACGCTGTCGCGCCTCAGCGTCAGCAACAGCCCAGCA 58152 0.30027192392450325 No Hit CCTGTTTGCTCCCCATGCTTTCGTACCTCAGCGTCAGTATTAGGCCAGAT 57497 0.29688978556003515 No Hit CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTTACTGCCCAGAG 55932 0.2888087984754663 No Hit CCTGTTTGCTCCCCACGCTGTCGCGCCTCAGTGTCAGCAACGGCCCAGCA 55810 0.28817884293277146 No Hit CCCGTTTGCTCCCCTGGCCTTCGTGCCTCAGCGTCAGGAACTGTCCAGAG 55411 0.28611857849395805 No Hit CCCGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAATGCCGGTCCAGGA 55306 0.2855764036416387 No Hit CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTACCGTCCCAGCG 54509 0.281461038334034 No Hit CCGGTTTGCTCCCCCAGCTTTCATCCCTCACCGTCGAGCGCGTTCTGGCA 53867 0.2781460263798531 No Hit CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGATCCAGTG 53150 0.27444374667401544 No Hit CCCGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAAGAACAGGCCAGGA 52786 0.2725642071859752 No Hit CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATATGTGTCCAGTG 52019 0.2686037489790331 No Hit CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTACCGTCCCAGCG 51857 0.26776725063545476 No Hit CCCGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGGAGATGCCCAGAG 51720 0.26705984154242857 No Hit CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAATTACGGGCCAGAT 50786 0.26223706714179773 No Hit CCCGTTCGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTGTCGGCCCAGCA 50733 0.261963397930627 No Hit CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTACCGTCCCAGCG 50002 0.25818882824448014 No Hit CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTACAGGTCCAGGG 49741 0.25684113646871504 No Hit CCTGTTTGCTCCCCACGCTGTCGCGCCTCAGCGTCAGCCACAGCCCAGCG 49339 0.2547653813198354 No Hit CCTGTTTGCTCCCCACGCTGTCGCGCCTCAGCGTCAGCAACGGCCCAGCA 48948 0.2527464254411987 No Hit CCTGTTTGCTCCCCACGCTTTCGCACTTGAGTGTCAGTACTGGACCAGAG 48881 0.25240046624971874 No Hit CCCGTTTGCTCCCCTGGCTTTCGCTCCTCAGTGTCAGTGTCGGTCCAGGA 48745 0.25169822072671466 No Hit CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTACCGTCCCAGAG 48646 0.25118702729452785 No Hit CCCGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGGAAATGCCCAGAG 46948 0.2424192853970212 No Hit CCTGTTTGATCCCCACACTTTCGTGCCTCAGCGTCAGTTACAGCCTAGTA 46192 0.23851562646032215 No Hit CCCGTTTGCTCCCCTGGCTTTCGCTCCTCAGTGTCAGTGTTGGTCCAGGA 44851 0.23159127906070115 No Hit CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTATTGGTCCAGGA 44837 0.23151898908039195 No Hit CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTCACCGTCCAGAG 44016 0.22727969809225712 No Hit CCCGTTTGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTGTCAGCCCAGCA 43707 0.22568415495543168 No Hit CCTGTTTGCTCCCCACGCTTTCGCGTATCAGCGTCAGTATCCAACCAGGG 43383 0.22401115826827492 No Hit CCCGTTTGCTCCCCTGGCTTTCGCTCCTCAGCGTCAGTATTGGTCCAGGA 43050 0.22229168945091937 No Hit CCCGTTTGCTCCCCTGGCTTTCGCTCCTCAGCGTCAATGCCGGTCCAGGA 42978 0.221919912409329 No Hit CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTTGTTGCCCAGAG 42838 0.2211970126062366 No Hit CCCGTTTGCTCCCCTGGCTTTCGCTCCTCAGTGTCAATGCCGGTCCAGGA 42694 0.2204534585230558 No Hit CCTGTTCGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTACCGTACTAGAG 42588 0.21990612010071442 No Hit CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTACCGGGCCAGTG 42229 0.21805239846278457 No Hit CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTGGCCCAGGA 41067 0.21205233009711746 No Hit CCCGTTTGCTCCCCTGGCCTTCGTGCCTCAGTGTCAGGAACTGTCCAGAG 39506 0.20399199729263695 No Hit CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTACAGGCCCAGGG 38889 0.20080607458900823 No Hit CCTGTTCGCTCCCCACGCTTTCGCGCCTTAGCGTCAGTACCGTCCCAGCG 38872 0.20071829389863272 No Hit CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTATCGGGCCAGTG 38758 0.20012964691611462 No Hit CCTGTTTGCTCCCCACGCTTTCGTGCATGAGTGTCAGTGTTGGCCCAGGA 37769 0.19502287616426886 No Hit CCCGTTTGCTCCCCTGGCTTTCGTTCCTCAGTGTCAGTTGCAGTCCAGAA 37676 0.1945426641522146 No Hit CCCGTTTGCTCCCCTAGCTTTCGCTCCTCAGCGTCAATGTCGGTCCAGGA 37597 0.1941347421204696 No Hit CCTGTTTGCTCCCCTAGCTTTCGTGCCTCAGTGTCAGGAGTTTTCCAGGG 37531 0.19379394649901174 No Hit CCCGTTCGCTCCCCTGGCTTTCGCGCATCAGCGTCAGTATCGGCCCAGTA 37310 0.19265279752413011 No Hit CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGAAATGTTCCAGAG 37101 0.1915736113895136 No Hit CCTGTTTGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTACCGGCCCAGAG 36964 0.19086620229648743 No Hit CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACCGGTCCAGTG 36791 0.1899729046826661 No Hit CCTGTTTGCTCCCCCGGCTTTCGTGCCTCAGTGTCAGGTGTTGTCCAGGG 36277 0.1873188296913125 No Hit CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTCACTGTCCAGAA 35802 0.18486613393082035 No Hit CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATATGTGTCCAGTA 35460 0.18310019298326602 No Hit CCGGTTTGCTCCCCCAGCTTTCATCCCTCACCGTCGAGCGCATTCTGGCA 34984 0.18064233365275179 No Hit CCCGTTTGCTACCCTAGCTTTCGCGCCTCAGCGTCAAGAACAGGCCAGGA 33788 0.17446670390633368 No Hit CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACGTGTCCAGTG 32912 0.16994341656698395 No Hit CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGAACCGGACCAGTA 32582 0.1682394384596947 No Hit CCCGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTAATGGTCCAGGA 32312 0.16684527455373072 No Hit CCCGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTATTGGGCCAGAT 32218 0.16635989897165437 No Hit CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTTACGTCCCAGCG 31923 0.1648366458151382 No Hit CCCGTTTGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTGTTGGCCCAGCA 31771 0.16405178317178074 No Hit CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTCATCGTCCAGAA 31182 0.16101044042877047 No Hit CCTGTTCGCTCCCCATGCTTTCGCTCCTCAGCGTCAGTTAATGCCCAGAG 30922 0.1596679122230274 No Hit CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGATCTGGACCAGTT 30819 0.15913606451075224 No Hit CCTGTTCGCTCCCCTAGCTTTCGCGCCTGAGCGTCAGGTAAGGCCCAGAG 30401 0.15697769224151917 No Hit CCTGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGGAACCGTCCAGAA 30290 0.15640453596906734 No Hit CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCGAGCCAGTG 30280 0.15635290026884643 No Hit CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAACAGTCGTCCAGTA 30016 0.154989717783015 No Hit CCCGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAAGAGTGGGCCAGGT 29710 0.15340966535625586 No Hit CCCGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGGAATTGCCCAGAG 29533 0.15249571346234617 No Hit CCCGTTCGCTCCCCTAGCTTTCGCGCCTCAGCGTCAAGAATGGGCCAGGT 29407 0.151845103639563 No Hit CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGT 29406 0.15183994006954088 No Hit CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTGTTGGTCCAGGA 28901 0.1492323372083861 No Hit CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACATGTCCAGTG 28721 0.14830289460441012 No Hit CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGCAAAGATCCAGCA 28426 0.14677964144789393 No Hit CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATTGGCCCAGGT 28107 0.14513246261084764 No Hit CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTATCGGGCCAGTG 27446 0.14171934282624699 No Hit CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTGGTCCAGTT 27400 0.14148181860523093 No Hit CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTGTCAGCCCAGGG 27279 0.1408570266325582 No Hit CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTGTCAGCCCAGCA 26425 0.13644733783369442 No Hit CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAATGGCCCAGAG 26191 0.13523906244852565 No Hit CCTGTTCGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGGTAAGGCCCAGAG 25931 0.1338965342427826 No Hit CCTGTTCGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTACCGTCCCAGAA 25861 0.1335350843412364 No Hit CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTGCTGGTCCAGGG 25854 0.13349893935108176 No Hit CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTACCGTCCCAGAG 25757 0.13299807305893915 No Hit CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTGCTGGTCCAGGT 25579 0.13207895759500737 No Hit CCCGTTCGCTCCCCTAGCTTTCGCGCTTCAGCGTCAGTGTCAGCCCAGCA 25553 0.13194470477443304 No Hit CCCGTTTGCTCCCCTAGCTTTCGCTCATCAGCGTCAATGCCGGTCCAGGA 24899 0.12856772997998703 No Hit CCCGTTTGCTCCCCTGGCTTTCGTGCCTCAGCGTCAGGAAATGTCCAGAG 24833 0.1282269343585292 No Hit CCCGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTCACCGTCCAGAA 24730 0.12769508664625404 No Hit CCTGTTTGCTCCCCTAGCTTTCGTGCCTCAGCGTCGGTATCTGTCCAGAA 24725 0.1276692687961436 No Hit CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAACAATCGTCCAGTA 24725 0.1276692687961436 No Hit CCTGTTTGCTCCCCACGCTTTCGCGCATGAGCGTCAGTGTTGGCCCAGAA 24301 0.12547991510677797 No Hit CCTGTTTGCTCCCCACGCTTTCGTGCCTGAACGTCGGTGCTGGTCCAGGA 24152 0.12471054317348676 No Hit CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTCCGGTCCAGTG 23063 0.11908741541943216 No Hit CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTATTGGCCCAGGA 22485 0.11610287194666487 No Hit CCCGTTTGCTCCCCTAGCTTTCGCTCCTCAGCGTCAATGCCGGTCCAGGA 22434 0.11583952987553835 No Hit CCTGTTCGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGGTCAGGCCCAGAG 21921 0.11319061845420683 No Hit CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTATCCCAGGG 21831 0.11272589715221884 No Hit CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTCACCGTCCAGAA 21529 0.11116649900554806 No Hit CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTGTCGGCCCAGCA 21527 0.11115617186550387 No Hit CCTGTTTGCTCCCCACGCTGTCGCGCCTCAGCGTCAGTACTGGCCCAGTC 21445 0.1107327591236926 No Hit CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTGTTGACCCAGGG 21286 0.1099117514901805 No Hit CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTCAGCCCAGGG 21276 0.1098601157899596 No Hit CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTGTTAGCCCAGGG 20641 0.10658124882593327 No Hit CCTGTTTGCTCCCCACGCTGTCGCGCCTCAGCGTCAGCAACGGCCCAGCG 20555 0.10613718180403364 No Hit CCCGTTTGCTCCCCTGGCTTTCGCTCCTCAGCGTCAATATTGGTCCAGGA 20523 0.1059719475633268 No Hit CCCGTTTGCTCCCCTGGCCTTCGTGCCTCAGTGTCAGGAACTGCCCAGAG 20235 0.10448483939696523 No Hit CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTGACCCAGGG 19875 0.10262595418901331 No Hit CCTGTTTGCTCCCCTAGCTTTCGTGCCTCAGTGTCAGGTGTTGTCCAGGG 19603 0.10122146314300517 No Hit CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACTTGTCCAGCG 19542 0.10090648537165778 No Hit CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTTACGGCCCAGAG 19526 0.10082386825130435 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 3.924313216787427E-4 0.0 0.0 2 3.924313216787427E-4 0.0 0.0 3 3.975948917008314E-4 0.0 0.0 4 4.0792203174500887E-4 0.0 0.0 5 4.182491717891863E-4 0.0 0.0 6 4.23412741811275E-4 0.0 0.0 7 4.285763118333637E-4 0.0 0.0 8 4.337398818554525E-4 0.0 0.0 9 4.337398818554525E-4 0.0 0.0 10-14 4.6265587397914933E-4 0.0 0.0 15-19 5.029317201514413E-4 0.0 0.0 20-24 5.752217004606835E-4 0.0 0.0 25-29 6.619696768317739E-4 0.0 0.0 30-34 8.282366315430306E-4 0.0 0.0 35-39 9.604240241085018E-4 0.0 0.0 40-44 0.0010874478466518845 0.0 0.0 45-49 0.0012764345094603315 0.0 0.0 50-54 0.001520155014502919 0.0 0.0 55-59 0.0018402963558724196 0.0 0.0 60-64 0.0022162042534804786 0.0 0.0 65-69 0.0026313552832564115 0.0 0.0 70-74 0.003199347985686171 0.0 0.0 75-79 0.00387267751656654 0.0 0.0 80-84 0.004791792980498332 0.0 0.0 85-89 0.0059949047956450036 0.0 0.0 90-94 0.007451031541874023 0.0 5.16357002208872E-6 95-99 0.009323342031883392 0.0 5.16357002208872E-6 100-104 0.011501335867200415 0.0 5.16357002208872E-6 105-109 0.014303088961185756 5.16357002208872E-6 5.16357002208872E-6 110-114 0.017588152209238598 5.16357002208872E-6 5.16357002208872E-6 115-119 0.021706615658856558 5.16357002208872E-6 8.261712035341952E-6 120-124 0.0263941045249087 5.16357002208872E-6 1.032714004417744E-5 125-129 0.03155251097697533 5.16357002208872E-6 1.032714004417744E-5 130-134 0.03715808259295485 5.16357002208872E-6 1.032714004417744E-5 135-139 0.04335539933346573 5.16357002208872E-6 1.032714004417744E-5 140-144 0.049736539166762975 5.16357002208872E-6 1.032714004417744E-5 145-149 0.05649358689766827 5.16357002208872E-6 1.032714004417744E-5 150-154 0.06329400861675911 5.16357002208872E-6 1.032714004417744E-5 155-159 0.07009236490784113 5.16357002208872E-6 1.032714004417744E-5 160-164 0.07710759113985086 5.16357002208872E-6 1.032714004417744E-5 165-169 0.08458030967581766 5.16357002208872E-6 1.032714004417744E-5 170-174 0.09165853146209688 5.16357002208872E-6 1.032714004417744E-5 175-179 0.09875017853043351 5.16357002208872E-6 1.032714004417744E-5 180-184 0.10624768220250633 5.16357002208872E-6 1.032714004417744E-5 185-189 0.11427600087284988 5.16357002208872E-6 1.858885207951939E-5 190-194 0.12214528158651308 5.16357002208872E-6 2.065428008835488E-5 195-199 0.1298173139253325 5.16357002208872E-6 2.065428008835488E-5 200-204 0.13799640884032105 5.16357002208872E-6 2.065428008835488E-5 205-209 0.1460660360708413 5.16357002208872E-6 2.065428008835488E-5 210-214 0.15435046781428047 5.16357002208872E-6 2.5817850110443595E-5 215-219 0.16261321256362682 5.16357002208872E-6 3.2014134136950064E-5 220-224 0.1708201907567346 5.16357002208872E-6 3.614499015462104E-5 225-229 0.17867914433035365 5.16357002208872E-6 3.614499015462104E-5 230-234 0.1864627097816502 5.16357002208872E-6 3.614499015462104E-5 235-239 0.19456641657431623 5.16357002208872E-6 3.614499015462104E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGTTTGC 954930 0.0 244.74088 3 CTGTTCG 200725 0.0 243.92589 2 TTTGCTC 1345405 0.0 243.89464 5 CTGTTTG 1018965 0.0 243.82065 2 GTTTGCT 1385570 0.0 243.67296 4 TGTTCGC 200150 0.0 242.44778 3 CCTGTTC 200365 0.0 242.27322 1 TTTGATC 63825 0.0 241.58362 5 CCTGTTT 1022120 0.0 241.52557 1 TGTTTGA 62075 0.0 241.0729 3 CCCGTTT 417255 0.0 240.25566 1 CCGTTTG 423965 0.0 238.17525 2 GTTCGCT 328415 0.0 236.6932 4 GTTTGAT 65665 0.0 236.19469 4 GCTCCCC 1727165 0.0 235.25484 8 TTGCTCC 1407830 0.0 234.68542 6 TGCTCCC 1406610 0.0 234.59637 7 GGTTTGC 16730 0.0 233.8703 3 TTTGCTA 55210 0.0 232.86314 5 CCGTTCG 117970 0.0 231.8331 2 >>END_MODULE