FastQCFastQC Report
Mon 14 Sep 2015
000000000-AJ2K7 l01n03.3330000003458e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJ2K7 l01n03.3330000003458e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19366446
Sequences flagged as poor quality0
Sequence length251
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTTTGCTCCCCTGGCTTTCGCGCCTCAGCGTCAATACCGGTCCAGGA3041281.5703862236777981No Hit
CCTGTTCGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTACCGTCCCAGAG2962761.5298418718643576No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGACCAGTG2045461.0561875937381593No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTGGCCCAGAA1979331.0220409051820865No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTAACGTCCCAGCG1751350.9043218358185079No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGGGCCAGTG1720860.8885781108211595No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGCAGGCCCAGGG1235250.6378299869785091No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTAGCCCAGGG1036300.535100761389054No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTATCGGCCCAGAG1035150.5345069508365138No Hit
CCTGTTCGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTACCGTTCCAGAG990220.5113070307272692No Hit
CCCGTTTGCTCCCCTGGCTTTCGCGCCTCAGCGTCAATGCCGGTCCAGGA986590.509432654809251No Hit
CCCGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTGTCGGTCCAGGA969470.5005926229314351No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGTGTCAGTATTGGGCCAGGA939130.484926351484418No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTGTCAGCCCAGCA916190.4730811218537464No Hit
CCCGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAAGAACAGGCCAGGT897940.4636576065634345No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTCAGCCCAGAG808850.4176553612366461No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTATTGGTCCAGGA768480.3968100290574739No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTATCCAACCAGGG727040.3754121948859383No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACCAGTCCAGTG706990.3650592369916504No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAACGTCGGTACTGGTCCAGGA648720.3349711144729394No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCGGGCCAGTG637810.32933765957884065No Hit
CCTGTTTGCTCCCCACGCTGTCGCACCTGAGTGTCAGTATTGGGCCAGGT616590.31838056399196835No Hit
CCCGTTTGCTCCCCTGGCTTTCGCTCCTCAGCGTCAGTGTTGGTCCAGGA610970.3154786376395545No Hit
CCTGTTTGCTCCCCACGCTGTCGCGCCTCAGCGTCAGCAACAGCCCAGCA581520.30027192392450325No Hit
CCTGTTTGCTCCCCATGCTTTCGTACCTCAGCGTCAGTATTAGGCCAGAT574970.29688978556003515No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTTACTGCCCAGAG559320.2888087984754663No Hit
CCTGTTTGCTCCCCACGCTGTCGCGCCTCAGTGTCAGCAACGGCCCAGCA558100.28817884293277146No Hit
CCCGTTTGCTCCCCTGGCCTTCGTGCCTCAGCGTCAGGAACTGTCCAGAG554110.28611857849395805No Hit
CCCGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAATGCCGGTCCAGGA553060.2855764036416387No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTACCGTCCCAGCG545090.281461038334034No Hit
CCGGTTTGCTCCCCCAGCTTTCATCCCTCACCGTCGAGCGCGTTCTGGCA538670.2781460263798531No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTACCGATCCAGTG531500.27444374667401544No Hit
CCCGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAAGAACAGGCCAGGA527860.2725642071859752No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATATGTGTCCAGTG520190.2686037489790331No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTACCGTCCCAGCG518570.26776725063545476No Hit
CCCGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGGAGATGCCCAGAG517200.26705984154242857No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAATTACGGGCCAGAT507860.26223706714179773No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTGTCGGCCCAGCA507330.261963397930627No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTACCGTCCCAGCG500020.25818882824448014No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTACAGGTCCAGGG497410.25684113646871504No Hit
CCTGTTTGCTCCCCACGCTGTCGCGCCTCAGCGTCAGCCACAGCCCAGCG493390.2547653813198354No Hit
CCTGTTTGCTCCCCACGCTGTCGCGCCTCAGCGTCAGCAACGGCCCAGCA489480.2527464254411987No Hit
CCTGTTTGCTCCCCACGCTTTCGCACTTGAGTGTCAGTACTGGACCAGAG488810.25240046624971874No Hit
CCCGTTTGCTCCCCTGGCTTTCGCTCCTCAGTGTCAGTGTCGGTCCAGGA487450.25169822072671466No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTACCGTCCCAGAG486460.25118702729452785No Hit
CCCGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGGAAATGCCCAGAG469480.2424192853970212No Hit
CCTGTTTGATCCCCACACTTTCGTGCCTCAGCGTCAGTTACAGCCTAGTA461920.23851562646032215No Hit
CCCGTTTGCTCCCCTGGCTTTCGCTCCTCAGTGTCAGTGTTGGTCCAGGA448510.23159127906070115No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTATTGGTCCAGGA448370.23151898908039195No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTCACCGTCCAGAG440160.22727969809225712No Hit
CCCGTTTGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTGTCAGCCCAGCA437070.22568415495543168No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTATCAGCGTCAGTATCCAACCAGGG433830.22401115826827492No Hit
CCCGTTTGCTCCCCTGGCTTTCGCTCCTCAGCGTCAGTATTGGTCCAGGA430500.22229168945091937No Hit
CCCGTTTGCTCCCCTGGCTTTCGCTCCTCAGCGTCAATGCCGGTCCAGGA429780.221919912409329No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTTGTTGCCCAGAG428380.2211970126062366No Hit
CCCGTTTGCTCCCCTGGCTTTCGCTCCTCAGTGTCAATGCCGGTCCAGGA426940.2204534585230558No Hit
CCTGTTCGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTACCGTACTAGAG425880.21990612010071442No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTACCGGGCCAGTG422290.21805239846278457No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTGGCCCAGGA410670.21205233009711746No Hit
CCCGTTTGCTCCCCTGGCCTTCGTGCCTCAGTGTCAGGAACTGTCCAGAG395060.20399199729263695No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTACAGGCCCAGGG388890.20080607458900823No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTTAGCGTCAGTACCGTCCCAGCG388720.20071829389863272No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTATCGGGCCAGTG387580.20012964691611462No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGTGTCAGTGTTGGCCCAGGA377690.19502287616426886No Hit
CCCGTTTGCTCCCCTGGCTTTCGTTCCTCAGTGTCAGTTGCAGTCCAGAA376760.1945426641522146No Hit
CCCGTTTGCTCCCCTAGCTTTCGCTCCTCAGCGTCAATGTCGGTCCAGGA375970.1941347421204696No Hit
CCTGTTTGCTCCCCTAGCTTTCGTGCCTCAGTGTCAGGAGTTTTCCAGGG375310.19379394649901174No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCATCAGCGTCAGTATCGGCCCAGTA373100.19265279752413011No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGAAATGTTCCAGAG371010.1915736113895136No Hit
CCTGTTTGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTACCGGCCCAGAG369640.19086620229648743No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACCGGTCCAGTG367910.1899729046826661No Hit
CCTGTTTGCTCCCCCGGCTTTCGTGCCTCAGTGTCAGGTGTTGTCCAGGG362770.1873188296913125No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTCACTGTCCAGAA358020.18486613393082035No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATATGTGTCCAGTA354600.18310019298326602No Hit
CCGGTTTGCTCCCCCAGCTTTCATCCCTCACCGTCGAGCGCATTCTGGCA349840.18064233365275179No Hit
CCCGTTTGCTACCCTAGCTTTCGCGCCTCAGCGTCAAGAACAGGCCAGGA337880.17446670390633368No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACGTGTCCAGTG329120.16994341656698395No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGAACCGGACCAGTA325820.1682394384596947No Hit
CCCGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTAATGGTCCAGGA323120.16684527455373072No Hit
CCCGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTATTGGGCCAGAT322180.16635989897165437No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTTACGTCCCAGCG319230.1648366458151382No Hit
CCCGTTTGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTGTTGGCCCAGCA317710.16405178317178074No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTCATCGTCCAGAA311820.16101044042877047No Hit
CCTGTTCGCTCCCCATGCTTTCGCTCCTCAGCGTCAGTTAATGCCCAGAG309220.1596679122230274No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGATCTGGACCAGTT308190.15913606451075224No Hit
CCTGTTCGCTCCCCTAGCTTTCGCGCCTGAGCGTCAGGTAAGGCCCAGAG304010.15697769224151917No Hit
CCTGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGGAACCGTCCAGAA302900.15640453596906734No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCGAGCCAGTG302800.15635290026884643No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAACAGTCGTCCAGTA300160.154989717783015No Hit
CCCGTTTGCTCCCCTAGCTTTCGCGCCTCAGCGTCAAGAGTGGGCCAGGT297100.15340966535625586No Hit
CCCGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGGAATTGCCCAGAG295330.15249571346234617No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGCCTCAGCGTCAAGAATGGGCCAGGT294070.151845103639563No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGT294060.15183994006954088No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTGTTGGTCCAGGA289010.1492323372083861No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACATGTCCAGTG287210.14830289460441012No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGCAAAGATCCAGCA284260.14677964144789393No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATTGGCCCAGGT281070.14513246261084764No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTATCGGGCCAGTG274460.14171934282624699No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTGGTCCAGTT274000.14148181860523093No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTGTCAGCCCAGGG272790.1408570266325582No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTGTCAGCCCAGCA264250.13644733783369442No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAATGGCCCAGAG261910.13523906244852565No Hit
CCTGTTCGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGGTAAGGCCCAGAG259310.1338965342427826No Hit
CCTGTTCGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTACCGTCCCAGAA258610.1335350843412364No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTGCTGGTCCAGGG258540.13349893935108176No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTACCGTCCCAGAG257570.13299807305893915No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTGCTGGTCCAGGT255790.13207895759500737No Hit
CCCGTTCGCTCCCCTAGCTTTCGCGCTTCAGCGTCAGTGTCAGCCCAGCA255530.13194470477443304No Hit
CCCGTTTGCTCCCCTAGCTTTCGCTCATCAGCGTCAATGCCGGTCCAGGA248990.12856772997998703No Hit
CCCGTTTGCTCCCCTGGCTTTCGTGCCTCAGCGTCAGGAAATGTCCAGAG248330.1282269343585292No Hit
CCCGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTCACCGTCCAGAA247300.12769508664625404No Hit
CCTGTTTGCTCCCCTAGCTTTCGTGCCTCAGCGTCGGTATCTGTCCAGAA247250.1276692687961436No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAACAATCGTCCAGTA247250.1276692687961436No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCATGAGCGTCAGTGTTGGCCCAGAA243010.12547991510677797No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAACGTCGGTGCTGGTCCAGGA241520.12471054317348676No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTCCGGTCCAGTG230630.11908741541943216No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTATTGGCCCAGGA224850.11610287194666487No Hit
CCCGTTTGCTCCCCTAGCTTTCGCTCCTCAGCGTCAATGCCGGTCCAGGA224340.11583952987553835No Hit
CCTGTTCGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGGTCAGGCCCAGAG219210.11319061845420683No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTATCCCAGGG218310.11272589715221884No Hit
CCTGTTTGCTCCCCACGCTTTCGCGTCTCAGCGTCAGTCACCGTCCAGAA215290.11116649900554806No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTGTCGGCCCAGCA215270.11115617186550387No Hit
CCTGTTTGCTCCCCACGCTGTCGCGCCTCAGCGTCAGTACTGGCCCAGTC214450.1107327591236926No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTGTTGACCCAGGG212860.1099117514901805No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTCAGCCCAGGG212760.1098601157899596No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTGTTAGCCCAGGG206410.10658124882593327No Hit
CCTGTTTGCTCCCCACGCTGTCGCGCCTCAGCGTCAGCAACGGCCCAGCG205550.10613718180403364No Hit
CCCGTTTGCTCCCCTGGCTTTCGCTCCTCAGCGTCAATATTGGTCCAGGA205230.1059719475633268No Hit
CCCGTTTGCTCCCCTGGCCTTCGTGCCTCAGTGTCAGGAACTGCCCAGAG202350.10448483939696523No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTGACCCAGGG198750.10262595418901331No Hit
CCTGTTTGCTCCCCTAGCTTTCGTGCCTCAGTGTCAGGTGTTGTCCAGGG196030.10122146314300517No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACTTGTCCAGCG195420.10090648537165778No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTTACGGCCCAGAG195260.10082386825130435No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTTGC9549300.0244.740883
CTGTTCG2007250.0243.925892
TTTGCTC13454050.0243.894645
CTGTTTG10189650.0243.820652
GTTTGCT13855700.0243.672964
TGTTCGC2001500.0242.447783
CCTGTTC2003650.0242.273221
TTTGATC638250.0241.583625
CCTGTTT10221200.0241.525571
TGTTTGA620750.0241.07293
CCCGTTT4172550.0240.255661
CCGTTTG4239650.0238.175252
GTTCGCT3284150.0236.69324
GTTTGAT656650.0236.194694
GCTCCCC17271650.0235.254848
TTGCTCC14078300.0234.685426
TGCTCCC14066100.0234.596377
GGTTTGC167300.0233.87033
TTTGCTA552100.0232.863145
CCGTTCG1179700.0231.83312
Produced by FastQC (version 0.11.2)