##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AJ2K7 l01n02.33200000034581.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 19366446 Sequences flagged as poor quality 0 Sequence length 12 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.984145051704374 32.0 29.0 34.0 16.0 34.0 2 30.359235091456636 33.0 32.0 34.0 16.0 34.0 3 30.461918103094394 33.0 32.0 34.0 16.0 34.0 4 30.526748996692525 33.0 32.0 34.0 16.0 34.0 5 30.595129534866643 33.0 32.0 34.0 16.0 34.0 6 32.90806279066381 37.0 33.0 37.0 18.0 37.0 7 32.48083664911982 35.0 32.0 37.0 16.0 37.0 8 32.469310683023615 35.0 32.0 37.0 16.0 37.0 9 32.536478401870944 35.0 32.0 37.0 16.0 37.0 10 32.477434837553574 35.0 32.0 37.0 16.0 37.0 11 32.244785904445244 34.0 32.0 37.0 16.0 37.0 12 32.5481093433457 37.0 32.0 37.0 16.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1474690644251453 1101 2 0.2618919638232917 1101 3 0.22140099395840096 1101 4 0.2821830065438995 1101 5 0.26609843610140516 1101 6 0.18116200423607864 1101 7 0.37014618306999836 1101 8 0.3303978235037306 1101 9 0.12239123548676645 1101 10 0.18081917895664645 1101 11 0.15264237240355527 1101 12 0.19096050527772945 1102 1 0.22729770695264406 1102 2 0.19601954322247295 1102 3 0.21403971641264974 1102 4 0.2789248316387507 1102 5 0.18587071780939723 1102 6 0.09032224625497065 1102 7 0.1715522758355874 1102 8 0.22170037561939182 1102 9 0.22565476056639255 1102 10 0.3037596814324246 1102 11 0.17662359096151192 1102 12 0.20896094450230862 1103 1 0.4244072004909931 1103 2 0.32450084851597794 1103 3 0.19804850937946128 1103 4 0.3659516293134608 1103 5 0.2576050014380904 1103 6 0.3292887174605781 1103 7 0.386180662604076 1103 8 0.45649617626859396 1103 9 0.16891366033691213 1103 10 0.1880427231256192 1103 11 0.10219095169851045 1103 12 0.14759862796942969 2112 1 0.11921119075813635 2112 2 0.03791521294635203 2112 3 -0.0150038700187487 2112 4 0.0899186288575109 2112 5 0.052203490186695234 2112 6 0.24072030065683947 2112 7 0.24620150799294294 2112 8 0.3715469915630081 2112 9 0.023644347966524037 2112 10 0.10906049715880073 2112 11 0.1504323589839558 2112 12 0.05954314879116396 1113 1 0.26147288715889516 1113 2 0.21048526549185453 1113 3 0.0657631738998603 1113 4 0.14052668914289157 1113 5 0.14900115367712985 1113 6 0.317258635469166 1113 7 0.37949496231001945 1113 8 0.24108132511685199 1113 9 0.05418371150789625 1113 10 0.04367675705032781 1113 11 0.022047387025430965 1113 12 0.17079838973715056 2113 1 -0.017718568625618047 2113 2 0.055346096846061954 2113 3 0.030990102935575692 2113 4 0.06201972350721974 2113 5 0.0982671853431718 2113 6 0.25801831353302873 2113 7 0.23311958323555615 2113 8 -0.26932595517545366 2113 9 0.06966631623022579 2113 10 0.12024439792858743 2113 11 0.15665236520111847 2113 12 -0.02398634423053636 1112 1 0.09613238692956116 1112 2 0.08963957205072859 1112 3 0.07965621645747234 1112 4 -0.0014068016799519967 1112 5 -0.11993237972239612 1112 6 -0.23523144535250395 1112 7 -0.13659340147597732 1112 8 -1.0434748354671335 1112 9 -0.21397472550404473 1112 10 -0.11253455440871107 1112 11 -0.16256719578098 1112 12 0.011240760852068377 2114 1 -0.191065787674642 2114 2 0.049521024442277906 2114 3 0.035575701304836116 2114 4 -0.1199542844666226 2114 5 0.06506503339088354 2114 6 -0.32433954664634257 2114 7 -0.37245121550654403 2114 8 -1.1271963528558615 2114 9 -0.16239295881754146 2114 10 0.023294143480804053 2114 11 0.021836240329015766 2114 12 0.04686791505731236 1114 1 0.33610259864444103 1114 2 0.14463929521424745 1114 3 0.17581606796037974 1114 4 0.22050594096865694 1114 5 0.152489571077993 1114 6 -0.04213723257433344 1114 7 -0.0035511805092056647 1114 8 0.3071804642203162 1114 9 0.07346008234538459 1114 10 0.12772731259882875 1114 11 0.14410894634585247 1114 12 0.07688131271525833 1109 1 0.23602145463843272 1109 2 0.06193403359132077 1109 3 0.06419771438925181 1109 4 0.13709267374748535 1109 5 0.12335958342985265 1109 6 -0.05241332519902642 1109 7 0.0748414576299794 1109 8 0.2017507026947598 1109 9 0.15468663771933677 1109 10 0.1316320320863369 1109 11 0.254052792037065 1109 12 0.11207893174780992 1108 1 0.16686415546300637 1108 2 0.06497791607625558 1108 3 0.1328041203961483 1108 4 -0.009641267378960094 1108 5 0.06889320899989215 1108 6 0.06902307258260976 1108 7 0.07245726244111239 1108 8 0.17437324808416577 1108 9 0.20430506505196888 1108 10 0.22346630773497367 1108 11 0.19104921543684839 1108 12 0.07335097873635021 1111 1 0.15221659478038774 1111 2 0.2543321975100987 1111 3 0.18171516757938022 1111 4 0.20885607781778148 1111 5 0.024168263072418483 1111 6 0.21213495394803772 1111 7 0.24293293601100174 1111 8 0.11689402981620134 1111 9 0.06517750083396123 1111 10 0.06806783046638998 1111 11 0.12030061540799863 1111 12 0.3027614094902802 1110 1 0.449612679023371 1110 2 0.12485373327684002 1110 3 0.3020261104246096 1110 4 0.13551737316209866 1110 5 0.2102406132816661 1110 6 0.3054412525142993 1110 7 0.3443272013729839 1110 8 0.30344750240882234 1110 9 -0.030299741520416035 1110 10 0.1111505380443063 1110 11 0.010926967100900242 1110 12 0.1696522609963651 1105 1 0.27245860098391717 1105 2 0.22933531559635867 1105 3 0.15909943582347452 1105 4 0.22583982861533158 1105 5 0.19022522946332288 1105 6 0.24418773128374482 1105 7 0.013986546931533894 1105 8 0.24357616589303888 1105 9 0.15341003838788225 1105 10 0.10458873330784968 1105 11 0.030945183864460546 1105 12 -0.022806827664048512 1104 1 -0.9151554498569325 1104 2 -0.2798304135312577 1104 3 -0.09372720616666186 1104 4 -0.10257939655022597 1104 5 -0.004281518843875176 1104 6 0.22514296100288078 1104 7 0.25525317858411256 1104 8 0.02956966519698767 1104 9 0.13095162294665386 1104 10 0.05328596879981973 1104 11 -0.3043548319602465 1104 12 0.12668843735038848 1107 1 -0.02569293316042831 1107 2 -0.27941279026824617 1107 3 -0.20119374874842322 1107 4 -0.07834459417875905 1107 5 -0.05900248018940957 1107 6 0.1296186719612038 1107 7 -0.056646359085561926 1107 8 -0.03510622755620574 1107 9 -0.17816627587991718 1107 10 0.0057760705552567515 1107 11 -0.07909830688038255 1107 12 -0.22872499396360269 1106 1 -0.17286138227701286 1106 2 -0.23885666240308012 1106 3 -0.15089224709160476 1106 4 -0.4367533374684882 1106 5 -0.25703292319068893 1106 6 -0.2817939038141688 1106 7 -0.36360160132492325 1106 8 -0.04905613281953691 1106 9 -0.10266041390996605 1106 10 -0.2924930066778444 1106 11 -0.2320475086338405 1106 12 -0.17801272317824868 2103 1 -0.17146015081464228 2103 2 -0.24579792764783903 2103 3 -0.1540519384621888 2103 4 -0.3749462887617021 2103 5 -0.31865163956137366 2103 6 -0.3231435173288304 2103 7 -0.4022695248768642 2103 8 -0.18979476420958719 2103 9 -0.24725773620445324 2103 10 -0.23486194213163714 2103 11 -0.1795185093213263 2103 12 -0.39266332366207024 2102 1 -0.428347583452819 2102 2 -0.3334330439713611 2102 3 -0.2741363720401928 2102 4 -0.4496745852268731 2102 5 -0.32129898562279635 2102 6 -0.3398482580988471 2102 7 -0.44245955182096 2102 8 -0.24295457340765836 2102 9 -0.318209434381842 2102 10 -0.26485611321680125 2102 11 -0.1517172184646114 2102 12 -0.28586568889815567 2101 1 -0.4244981048016072 2101 2 -0.20470555450402372 2101 3 -0.4075850328447608 2101 4 -0.1897166423515202 2101 5 -0.12337164450442373 2101 6 -0.1211629198772215 2101 7 -0.1523444171730759 2101 8 0.04432244661453666 2101 9 -0.11815816049585237 2101 10 -0.17860479282869335 2101 11 -0.10660343046436083 2101 12 -0.07772237792357828 2107 1 -0.3318353489914436 2107 2 -0.10320267138388317 2107 3 -0.23332242034072337 2107 4 -0.12040890663364934 2107 5 -0.11289364456393969 2107 6 -0.1292945179971099 2107 7 -0.02939085047514567 2107 8 -0.033401345197681565 2107 9 -0.03610287826629843 2107 10 -0.15931727421206432 2107 11 -0.1269328355127044 2107 12 -0.0067493644700107325 2106 1 -0.04416034152236037 2106 2 -0.0288466198939048 2106 3 -0.047372186748333434 2106 4 -0.019293036357822757 2106 5 -0.11650685666197091 2106 6 -0.018579764443963143 2106 7 -0.12839437353626693 2106 8 -0.17489030997693789 2106 9 -0.07020763097560234 2106 10 -0.09409258249940677 2106 11 -0.10476693427192174 2106 12 -0.11173531643687085 2105 1 0.039082577688926534 2105 2 -0.3324359549582425 2105 3 -0.31628966930830416 2105 4 -0.01111194732602172 2105 5 -0.16173333465143003 2105 6 -0.028006495087041117 2105 7 -0.12690978328701163 2105 8 0.03055037313988862 2105 9 -0.0471478550316462 2105 10 -0.04610619231736024 2105 11 -0.06277500513684231 2105 12 0.1547460484828065 2104 1 -0.22446722305687317 2104 2 -0.061360450625535634 2104 3 -0.1805833331188076 2104 4 -0.36289603249452185 2104 5 -0.15151195664704886 2104 6 -0.15390079644323862 2104 7 -0.1136221096197616 2104 8 -0.14987019852675587 2104 9 0.014534842296107797 2104 10 -0.14622315263329 2104 11 -0.04418020041197934 2104 12 -0.5230402370871161 2111 1 -0.0829960567690442 2111 2 -0.019430148112043355 2111 3 0.19127354985868905 2111 4 0.040191549831305906 2111 5 0.03759777184222912 2111 6 -0.023823774128487685 2111 7 0.1744511574827854 2111 8 0.008016945694294009 2111 9 0.03405215998678557 2111 10 0.09747279359790895 2111 11 0.08715317761616603 2111 12 0.1730644466984046 2110 1 0.09656599627574991 2110 2 0.07272766028819433 2110 3 -0.02524700738380048 2110 4 0.15602045081107008 2110 5 0.03194331890634672 2110 6 -0.05595700321015329 2110 7 -0.2160078567611805 2110 8 0.250600077474175 2110 9 0.197134615452498 2110 10 -0.08101529342926028 2110 11 -0.1098101292910485 2110 12 -0.21152807842210564 2109 1 0.18957406260024 2109 2 -0.12220302122208082 2109 3 0.06434235701112101 2109 4 0.075417769354587 2109 5 0.048312128244891284 2109 6 -0.018014837740260248 2109 7 -0.2173928969701393 2109 8 0.11165667070471841 2109 9 -0.0128597564038202 2109 10 0.05136750340493279 2109 11 0.0872035040015362 2109 12 0.08031882822245251 2108 1 -0.18423022581031745 2108 2 0.07139557962906906 2108 3 -0.01734390551899878 2108 4 -0.14223905243690282 2108 5 -0.2151233421060894 2108 6 -0.45467152296220803 2108 7 -0.20330979307910724 2108 8 -0.12809028882060147 2108 9 -0.1547290297241517 2108 10 -0.33332756537472363 2108 11 -0.04379356228386655 2108 12 -0.04267767069084272 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 103.0 15 3181.0 16 79261.0 17 184578.0 18 274826.0 19 374854.0 20 379152.0 21 434370.0 22 434656.0 23 413939.0 24 404079.0 25 389323.0 26 376339.0 27 381599.0 28 391312.0 29 439447.0 30 493853.0 31 674465.0 32 1007944.0 33 2200886.0 34 4099005.0 35 5288950.0 36 640324.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 22.75015355714249 25.135957777014116 36.13015862278543 15.983730043057964 2 22.88060493907865 28.128552858898324 15.979157972505643 33.011684229517385 3 12.857728258452791 30.376946807896505 37.70147088422935 19.063854049421355 4 15.624632418359052 9.434539512309073 37.2087527055816 37.73207536375027 5 24.312163419142575 43.26870299279486 13.003795327237636 19.415338260824935 6 34.05675465699798 14.699108964029847 20.782625784823917 30.46151059414825 7 38.3650722491881 19.41804913508653 29.3666840059348 12.850194609790563 8 24.486501033798355 16.053327492302923 26.526038902543085 32.93413257135563 9 14.69500908943231 29.82306614233711 38.086058743044546 17.395866025186034 10 27.75467711057332 24.255660977306935 27.72634459893618 20.263317313183567 11 35.16165537032453 22.011849773572294 12.34817684153303 30.47831801457015 12 11.409413993667192 34.050491246561194 23.934061004275126 30.606033755496494 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2383.0 1 2383.0 2 2383.0 3 2383.0 4 7435.5 5 12488.0 6 12488.0 7 12488.0 8 12488.0 9 12488.0 10 12488.0 11 12488.0 12 12488.0 13 26677.0 14 40866.0 15 40866.0 16 40866.0 17 40866.0 18 40866.0 19 40866.0 20 40866.0 21 79629.0 22 118392.0 23 118392.0 24 118392.0 25 118392.0 26 118392.0 27 118392.0 28 118392.0 29 273671.5 30 428951.0 31 428951.0 32 428951.0 33 428951.0 34 428951.0 35 428951.0 36 428951.0 37 428951.0 38 3421058.5 39 6413166.0 40 6413166.0 41 6413166.0 42 6413166.0 43 6413166.0 44 6413166.0 45 6413166.0 46 7116818.5 47 7820471.0 48 7820471.0 49 7820471.0 50 7820471.0 51 7820471.0 52 7820471.0 53 7820471.0 54 6038889.5 55 4257308.0 56 4257308.0 57 4257308.0 58 4257308.0 59 4257308.0 60 4257308.0 61 4257308.0 62 4257308.0 63 2238612.5 64 219917.0 65 219917.0 66 219917.0 67 219917.0 68 219917.0 69 219917.0 70 219917.0 71 129948.5 72 39980.0 73 39980.0 74 39980.0 75 39980.0 76 39980.0 77 39980.0 78 39980.0 79 24049.5 80 8119.0 81 8119.0 82 8119.0 83 8119.0 84 8119.0 85 8119.0 86 8119.0 87 8119.0 88 4982.0 89 1845.0 90 1845.0 91 1845.0 92 1845.0 93 1845.0 94 1845.0 95 1845.0 96 2202.5 97 2560.0 98 2560.0 99 2560.0 100 2560.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.003568026885263305 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 1.032714004417744E-5 11 0.0 12 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 12 1.9366446E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 3.5730142279964614 #Duplication Level Percentage of deduplicated Percentage of total 1 65.79361359864822 2.3508151749927153 2 13.683310791680995 0.9778132828954743 3 6.25417402084251 0.6703875828250837 4 3.1251099599453926 0.4466424940375336 5 2.1501997484223225 0.3841347147073685 6 1.4776576954063472 0.3167815181877204 7 1.0830103345052857 0.27087279339782144 8 0.753942744488036 0.21550785225203628 9 0.7147486065198208 0.22984262464823677 >10 4.180465009517185 2.9021356331964334 >50 0.37508309223168584 0.9306591051018029 >100 0.273039294581754 2.1282404714696326 >500 0.07278144604956555 1.8655050697435622 >1k 0.05643937302163003 3.5244824765460003 >5k 0.0012848568328870688 0.3139605140064508 >10k+ 0.005139427306554255 82.47221869199228 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATCGCTCGAGGA 591479 3.054143233095014 No Hit TGGCAAGACTCT 571803 2.9525448293403964 No Hit GGTCACTGACAG 554259 2.8619551568728716 No Hit CGACTGTCTTAA 547470 2.8268996799929114 No Hit CCATGCGATAAC 533366 2.754072688401372 No Hit AATCTTGCTGCA 521683 2.6937466998333095 No Hit CCTCTCGTGATC 517695 2.6731543825852198 No Hit TCGTCGATAATC 516360 2.6662610166057314 No Hit TGCAATGTTGCT 515848 2.6636172687544217 No Hit CAACACGCACGA 510057 2.633715034756506 No Hit CCATAGGGTTCA 503734 2.601065781506839 No Hit TCGGAGTGTTGT 498897 2.576089593309996 No Hit TAATCCACAGCG 491377 2.5372595467438885 No Hit AATGGAGCATGA 490380 2.5321114674318665 No Hit GTCTACACACAT 485732 2.508111193969198 No Hit TATCAGGTGTGC 481241 2.4849216009999977 No Hit GTACCTAATTGC 474069 2.447888476801577 No Hit GCCTGAATTTAC 464436 2.3981478067787965 No Hit AGCTTGACAGCT 464106 2.396443828671507 No Hit ATGTGGGACCCA 459766 2.3740339347756425 No Hit TGTTATCGCACA 458029 2.3650648136472743 No Hit GCATGGCTCTAA 455156 2.3502298769738132 No Hit ACTCACGGTATG 451781 2.332802828149264 No Hit TGTAATTGTCGC 443124 2.288101802468042 No Hit TACTAATCTGCG 424021 2.1894621243360812 No Hit GCTCAGTGCAGA 423292 2.1856978817899786 No Hit TCTTCCGCTACT 411839 2.1265595143269964 No Hit TCAAGCTCAAGC 410523 2.1197642561779273 No Hit TAACGCTTGGGT 408933 2.1115541798428064 No Hit ATTGGGCTAGGC 392111 2.02469260493123 No Hit GATTGGTTGCAC 381517 1.969989744117222 No Hit GAAGAAGCGGTA 353144 1.8234837718804988 No Hit AATCCGTACAGC 271232 1.4005254242311675 No Hit AATCAGTCTCGT 271087 1.399776706577965 No Hit GGAGACAAGGGA 227994 1.1772629836160957 No Hit ACACCTGGTGAT 190623 0.984295208320618 No Hit >>END_MODULE >>Adapter Content warn >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGTCGG 210 0.0 6.000207 1 TCATAGG 485 0.0 6.000207 1 CGACTGG 105 4.828198E-7 6.000207 1 ACTCAGT 395 0.0 6.000207 1 CCATTCG 95 3.4281165E-6 6.000207 1 TATCAGT 835 0.0 6.0002065 1 AGTCAGT 55 0.009157721 6.0002065 1 TCTTCGG 260 0.0 6.0002065 1 TATGGAG 430 0.0 6.0002065 1 GATCCGT 75 1.7506328E-4 6.0002065 1 ATTCGGG 100 1.28594E-6 6.0002065 1 TAGCAGG 55 0.009157721 6.0002065 1 TCGGAGT 51945 0.0 6.0002065 1 GCCTGGA 115 6.823211E-8 6.0002065 1 CCATCGG 145 1.9645086E-10 6.0002065 1 CCTCAGT 180 0.0 6.0002065 1 CCTCAGG 80 6.5365086E-5 6.0002065 1 TCGTCCG 65 0.0012615003 6.0002065 1 TATTAGG 415 0.0 6.0002065 1 TCGTAGG 75 1.7506328E-4 6.0002065 1 >>END_MODULE