FastQCFastQC Report
Mon 14 Sep 2015
000000000-AJ2K7 l01n02.33200000034581.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AJ2K7 l01n02.33200000034581.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19366446
Sequences flagged as poor quality0
Sequence length12
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGCTCGAGGA5914793.054143233095014No Hit
TGGCAAGACTCT5718032.9525448293403964No Hit
GGTCACTGACAG5542592.8619551568728716No Hit
CGACTGTCTTAA5474702.8268996799929114No Hit
CCATGCGATAAC5333662.754072688401372No Hit
AATCTTGCTGCA5216832.6937466998333095No Hit
CCTCTCGTGATC5176952.6731543825852198No Hit
TCGTCGATAATC5163602.6662610166057314No Hit
TGCAATGTTGCT5158482.6636172687544217No Hit
CAACACGCACGA5100572.633715034756506No Hit
CCATAGGGTTCA5037342.601065781506839No Hit
TCGGAGTGTTGT4988972.576089593309996No Hit
TAATCCACAGCG4913772.5372595467438885No Hit
AATGGAGCATGA4903802.5321114674318665No Hit
GTCTACACACAT4857322.508111193969198No Hit
TATCAGGTGTGC4812412.4849216009999977No Hit
GTACCTAATTGC4740692.447888476801577No Hit
GCCTGAATTTAC4644362.3981478067787965No Hit
AGCTTGACAGCT4641062.396443828671507No Hit
ATGTGGGACCCA4597662.3740339347756425No Hit
TGTTATCGCACA4580292.3650648136472743No Hit
GCATGGCTCTAA4551562.3502298769738132No Hit
ACTCACGGTATG4517812.332802828149264No Hit
TGTAATTGTCGC4431242.288101802468042No Hit
TACTAATCTGCG4240212.1894621243360812No Hit
GCTCAGTGCAGA4232922.1856978817899786No Hit
TCTTCCGCTACT4118392.1265595143269964No Hit
TCAAGCTCAAGC4105232.1197642561779273No Hit
TAACGCTTGGGT4089332.1115541798428064No Hit
ATTGGGCTAGGC3921112.02469260493123No Hit
GATTGGTTGCAC3815171.969989744117222No Hit
GAAGAAGCGGTA3531441.8234837718804988No Hit
AATCCGTACAGC2712321.4005254242311675No Hit
AATCAGTCTCGT2710871.399776706577965No Hit
GGAGACAAGGGA2279941.1772629836160957No Hit
ACACCTGGTGAT1906230.984295208320618No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTCGG2100.06.0002071
TCATAGG4850.06.0002071
CGACTGG1054.828198E-76.0002071
ACTCAGT3950.06.0002071
CCATTCG953.4281165E-66.0002071
TATCAGT8350.06.00020651
AGTCAGT550.0091577216.00020651
TCTTCGG2600.06.00020651
TATGGAG4300.06.00020651
GATCCGT751.7506328E-46.00020651
ATTCGGG1001.28594E-66.00020651
TAGCAGG550.0091577216.00020651
TCGGAGT519450.06.00020651
GCCTGGA1156.823211E-86.00020651
CCATCGG1451.9645086E-106.00020651
CCTCAGT1800.06.00020651
CCTCAGG806.5365086E-56.00020651
TCGTCCG650.00126150036.00020651
TATTAGG4150.06.00020651
TCGTAGG751.7506328E-46.00020651
Produced by FastQC (version 0.11.2)