##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AJ2K7 l01n01.33100000034584.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 19366446 Sequences flagged as poor quality 0 Sequence length 251 %GC 56 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.28357546862238 33.0 32.0 33.0 32.0 34.0 2 32.4842215758121 33.0 32.0 33.0 32.0 34.0 3 32.62400938200019 33.0 32.0 34.0 32.0 34.0 4 32.20997915673325 33.0 32.0 34.0 32.0 34.0 5 31.46140974962572 33.0 32.0 33.0 29.0 34.0 6 33.80829156779721 35.0 33.0 37.0 31.0 37.0 7 34.36395242575742 37.0 33.0 37.0 32.0 37.0 8 32.45833840654088 33.0 32.0 34.0 31.0 35.0 9 32.60391540089493 33.0 32.0 34.0 30.0 37.0 10-14 33.855048004161425 35.0 33.4 36.4 30.2 37.0 15-19 37.093809685060435 38.0 37.6 38.0 35.8 38.0 20-24 36.77652622479106 38.2 37.0 38.2 34.0 38.2 25-29 36.31608875474622 38.2 36.8 38.2 33.8 38.2 30-34 37.87742547083756 39.0 38.2 39.0 36.6 39.0 35-39 37.45650824110939 38.4 38.2 38.4 36.4 38.4 40-44 37.2601115351779 38.6 37.8 38.6 35.6 38.8 45-49 36.230056645395855 38.0 36.6 38.0 33.2 38.4 50-54 37.162964366306554 38.2 37.8 38.2 35.8 38.8 55-59 35.32886416020782 38.0 36.4 38.2 26.8 38.2 60-64 36.165422597414114 38.2 36.8 39.0 30.2 39.0 65-69 35.941062371485195 38.4 36.6 38.8 27.2 39.0 70-74 36.307278454704594 38.2 36.6 38.8 30.8 39.0 75-79 37.07375221039524 39.0 37.4 39.0 35.2 39.0 80-84 35.733563287760695 38.2 36.6 38.4 27.0 39.0 85-89 36.70144264982847 38.4 37.0 38.8 33.8 39.0 90-94 36.0052174467117 38.4 36.6 39.0 28.0 39.0 95-99 34.9648128830659 38.0 35.2 38.4 24.6 39.0 100-104 35.285331588459755 38.0 35.4 39.0 23.0 39.0 105-109 35.120072934393846 38.0 35.4 38.8 24.4 39.0 110-114 35.91034572889625 38.4 36.6 39.0 26.8 39.0 115-119 35.078024496595816 38.0 35.0 38.8 24.2 39.0 120-124 35.35442449275412 38.0 36.8 39.0 25.2 39.0 125-129 34.29733983199602 37.8 34.0 38.4 18.0 39.0 130-134 35.120209872270834 37.6 35.2 38.0 27.2 39.0 135-139 33.48937254672334 37.2 33.4 38.0 16.2 39.0 140-144 35.127347991469364 37.2 35.8 38.0 27.8 39.0 145-149 35.09165625949129 37.6 35.8 38.6 24.6 39.0 150-154 34.87411747101146 37.0 35.2 38.0 26.8 39.0 155-159 34.70785581412305 37.0 35.0 38.0 27.4 38.4 160-164 34.72303940537154 37.0 35.6 38.0 28.2 38.0 165-169 34.38135559823419 37.0 35.0 38.0 27.0 38.0 170-174 34.33969649361582 37.0 36.0 38.0 24.8 38.0 175-179 33.427435441691266 37.0 33.8 37.2 18.4 38.0 180-184 33.37747700326637 36.6 33.0 37.0 21.0 38.0 185-189 33.76338925582938 37.0 34.4 37.0 21.8 37.8 190-194 33.510366414157765 37.0 33.0 37.0 23.6 37.0 195-199 32.016561758414525 34.6 31.0 37.0 19.4 37.0 200-204 31.430603457134055 35.6 29.4 37.0 13.0 37.0 205-209 33.30731522965029 37.0 33.0 37.0 22.6 37.0 210-214 31.788595078312255 36.8 30.6 37.0 13.6 37.0 215-219 32.46250915630054 36.8 30.6 37.0 17.8 37.0 220-224 33.29833428394657 37.0 33.2 37.0 20.0 37.0 225-229 32.11292481852375 36.6 31.6 37.0 13.6 37.0 230-234 31.02308312015534 34.8 28.0 37.0 17.0 37.0 235-239 30.794228863674835 34.6 28.0 37.0 13.0 37.0 240-244 31.72374495557936 35.2 29.6 37.0 19.8 37.0 245-249 31.18727547635741 35.2 28.4 37.0 13.6 37.0 250-251 28.721391059567665 33.5 21.5 36.0 12.5 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.11190191059306187 1101 2 0.2016422385219414 1101 3 0.1867393449317447 1101 4 0.27522545519107666 1101 5 0.4179626283438722 1101 6 0.29453516617476794 1101 7 0.3273024837843508 1101 8 0.33253071481769325 1101 9 0.39730511838267546 1101 10-14 0.17556464574765585 1101 15-19 0.28448028553543026 1101 20-24 0.25743501746346453 1101 25-29 0.5453065827061323 1101 30-34 0.24024388752616943 1101 35-39 0.21635359502193552 1101 40-44 0.264527044446055 1101 45-49 0.24485829649746194 1101 50-54 0.23715843521561908 1101 55-59 0.22609544756230093 1101 60-64 0.22539390429408712 1101 65-69 0.25513563058505895 1101 70-74 0.4145637575236023 1101 75-79 0.18752969815331255 1101 80-84 0.2108487444917344 1101 85-89 0.24980680676205225 1101 90-94 0.31221190201291193 1101 95-99 0.38226861608396234 1101 100-104 0.3809060990377944 1101 105-109 0.36019709325422156 1101 110-114 0.2608199679046166 1101 115-119 0.41341752922303954 1101 120-124 0.3206789450919487 1101 125-129 0.4553695327578211 1101 130-134 0.35881104034609734 1101 135-139 0.4790700707558031 1101 140-144 0.41706349205223603 1101 145-149 0.3308026762309666 1101 150-154 0.23632275834620486 1101 155-159 0.36612572990966896 1101 160-164 0.34120907216439633 1101 165-169 0.4641082210350973 1101 170-174 0.3571172080422613 1101 175-179 0.4470316618569896 1101 180-184 0.4338897785420315 1101 185-189 0.5116685271090589 1101 190-194 0.5280117414038514 1101 195-199 0.43543080859816996 1101 200-204 0.5268310720907152 1101 205-209 0.6864699832886885 1101 210-214 0.5489719001123063 1101 215-219 0.3469910502046645 1101 220-224 0.3616912916702404 1101 225-229 0.43353671495456325 1101 230-234 0.5445041006834543 1101 235-239 0.6891975215927069 1101 240-244 0.7684774064570377 1101 245-249 0.19781522353305547 1101 250-251 -0.11962582818772916 1102 1 0.13125246933443435 1102 2 0.025177148099075453 1102 3 -0.024200585837441224 1102 4 0.05222535835299169 1102 5 0.23922369709920588 1102 6 0.06648604753060283 1102 7 0.14729798464176724 1102 8 0.15232749702244064 1102 9 0.21333163853633152 1102 10-14 0.1351187932265958 1102 15-19 0.13356719539859085 1102 20-24 0.1693645258020382 1102 25-29 0.363727698850667 1102 30-34 0.1930350845268407 1102 35-39 0.11375990555193738 1102 40-44 0.24193198846402453 1102 45-49 0.20964122294306975 1102 50-54 0.14520159841186597 1102 55-59 0.20908040447100262 1102 60-64 0.1057779703057733 1102 65-69 0.16408042774838094 1102 70-74 0.34283988900757834 1102 75-79 0.15415385727578723 1102 80-84 0.23502944677506576 1102 85-89 0.18779466161176828 1102 90-94 0.3078314749800555 1102 95-99 0.3184666965014742 1102 100-104 0.43394644343415933 1102 105-109 0.17746320354941503 1102 110-114 0.10168397091371872 1102 115-119 0.14005871814364212 1102 120-124 0.3684665567197314 1102 125-129 0.40525015224410055 1102 130-134 0.22245635036384925 1102 135-139 0.4114175942048064 1102 140-144 0.2697085675438302 1102 145-149 0.2985044919277726 1102 150-154 0.15302754324522994 1102 155-159 0.08219401583124863 1102 160-164 0.0829171588201234 1102 165-169 0.29592293448683193 1102 170-174 0.047261649883779455 1102 175-179 0.07048909763373246 1102 180-184 0.2508193859174668 1102 185-189 0.3624306710861802 1102 190-194 0.48747394638158426 1102 195-199 0.20449002679709594 1102 200-204 0.6361885460007279 1102 205-209 0.5468858106696004 1102 210-214 -0.5865703101270974 1102 215-219 0.11278146474742812 1102 220-224 0.3381553652607323 1102 225-229 0.4273891245175676 1102 230-234 0.4983303649475914 1102 235-239 0.7318304218178753 1102 240-244 0.5801101291426676 1102 245-249 0.16402007022218612 1102 250-251 0.21031544387249923 1103 1 0.14377876207547047 1103 2 0.10147839057996322 1103 3 0.14520874519246973 1103 4 0.2522621788916837 1103 5 0.4546893447142857 1103 6 0.33255610834426363 1103 7 0.28829781204698435 1103 8 0.3491386098109004 1103 9 0.5070214238012838 1103 10-14 0.2912542633105346 1103 15-19 0.07415434338120974 1103 20-24 0.1825453662262433 1103 25-29 0.18660046381388895 1103 30-34 -0.12535810917332668 1103 35-39 0.12235139502387682 1103 40-44 0.32798746893081443 1103 45-49 0.2140863453779147 1103 50-54 0.1862849817441088 1103 55-59 0.3433880968151044 1103 60-64 0.253010838412834 1103 65-69 0.240974507941786 1103 70-74 0.417656109734871 1103 75-79 0.33632323903178474 1103 80-84 0.36401252680386875 1103 85-89 0.34886794155352874 1103 90-94 0.35540547371465436 1103 95-99 0.5629503852864488 1103 100-104 0.40786841589799394 1103 105-109 0.1660820192995871 1103 110-114 0.46055103486954607 1103 115-119 0.35560475585057105 1103 120-124 0.2060510156865618 1103 125-129 -0.1532712700879486 1103 130-134 0.3248840614437114 1103 135-139 0.11776376171226843 1103 140-144 0.4569549877840018 1103 145-149 0.2809798429035766 1103 150-154 0.34181588425253295 1103 155-159 0.18608383584632548 1103 160-164 0.4588641045125925 1103 165-169 0.41071480857205245 1103 170-174 0.28147123571171306 1103 175-179 0.4232923629948573 1103 180-184 0.3617925614859061 1103 185-189 0.52307997814016 1103 190-194 0.6620982999751632 1103 195-199 0.42315945001903543 1103 200-204 0.3734540603189487 1103 205-209 0.07909164789275991 1103 210-214 -0.04083564494720093 1103 215-219 0.29444827684889674 1103 220-224 0.5152234927056796 1103 225-229 -0.16836540172515058 1103 230-234 -0.3737686226430341 1103 235-239 0.8802446579657577 1103 240-244 0.784778035336366 1103 245-249 0.3433402423843397 1103 250-251 0.4300747603130155 2112 1 0.051070223740353526 2112 2 0.010676735810854154 2112 3 -0.02034426406649459 2112 4 0.04400712905842141 2112 5 0.005666805260087671 2112 6 -0.03678938616045713 2112 7 -0.129450204056333 2112 8 -0.010630404284121653 2112 9 -0.0030508881894135698 2112 10-14 -0.19369734120824944 2112 15-19 -0.09375935983553774 2112 20-24 0.22514028393721475 2112 25-29 0.3080641110914968 2112 30-34 -0.2236321393551961 2112 35-39 0.06917499793335224 2112 40-44 -0.0067068324262464785 2112 45-49 -0.058800681440573044 2112 50-54 0.09358144800730628 2112 55-59 0.2210103815779476 2112 60-64 0.09144412353166587 2112 65-69 0.21109336757942998 2112 70-74 0.3198783388772668 2112 75-79 0.1309871801883773 2112 80-84 0.31364401892385985 2112 85-89 0.25117027026178107 2112 90-94 0.30944636539077663 2112 95-99 0.05644002436307005 2112 100-104 0.23075048396359676 2112 105-109 0.2653917920009192 2112 110-114 0.2842084703118317 2112 115-119 0.02355324196506814 2112 120-124 0.3267287746239589 2112 125-129 0.18327830233464937 2112 130-134 0.11654771043713907 2112 135-139 0.24824588148966598 2112 140-144 0.2749622739371347 2112 145-149 0.2634573121884429 2112 150-154 0.1346365015814257 2112 155-159 0.20661098852196602 2112 160-164 0.187722597045628 2112 165-169 -0.0803224474912625 2112 170-174 -0.09720027615229299 2112 175-179 0.12720347020870548 2112 180-184 -0.030514084867974134 2112 185-189 0.36776876470968745 2112 190-194 0.4250852862074197 2112 195-199 0.21217078719158167 2112 200-204 -0.05052107755311752 2112 205-209 0.1392300250488887 2112 210-214 0.52436728567465 2112 215-219 0.3228649871763096 2112 220-224 0.22112910566249866 2112 225-229 0.3735622988188254 2112 230-234 -0.06747978917142561 2112 235-239 0.5933644163333582 2112 240-244 0.4414516774199626 2112 245-249 0.1774061211003044 2112 250-251 0.393681755764689 1113 1 0.18044628173517197 1113 2 0.1618246930669116 1113 3 0.16726884299662714 1113 4 0.2312655877387968 1113 5 0.2843440535854391 1113 6 0.2299376774412707 1113 7 0.01785555061503885 1113 8 0.1860179142557783 1113 9 0.3385485556170451 1113 10-14 0.09172514329317494 1113 15-19 0.2239942674586004 1113 20-24 0.2325629845639412 1113 25-29 0.3147341830371886 1113 30-34 -0.1662949669617646 1113 35-39 0.1934839814214655 1113 40-44 0.14337420648687527 1113 45-49 0.04553525752699272 1113 50-54 0.24190436788110503 1113 55-59 0.2401248671072196 1113 60-64 0.2545010235000902 1113 65-69 -0.10320472956153992 1113 70-74 0.2981311285959052 1113 75-79 0.17265455312498545 1113 80-84 0.3359100344992143 1113 85-89 0.2524780307687351 1113 90-94 0.2307614784282137 1113 95-99 0.09380843793558569 1113 100-104 0.1755344865742714 1113 105-109 0.4149624969878474 1113 110-114 0.24407247441828162 1113 115-119 0.4052523540030819 1113 120-124 0.4243414110934367 1113 125-129 0.37082916086211526 1113 130-134 0.35149637629614716 1113 135-139 0.6260341264141402 1113 140-144 0.5011231996398706 1113 145-149 0.4010862769910233 1113 150-154 0.40516981901164684 1113 155-159 0.4856971261421421 1113 160-164 0.35187114828373467 1113 165-169 0.3413759230331621 1113 170-174 0.3507415995248948 1113 175-179 0.4006780972876456 1113 180-184 -0.17871197449017728 1113 185-189 0.42730832836694077 1113 190-194 0.6586276279599232 1113 195-199 0.4196478552555405 1113 200-204 0.7417827528654435 1113 205-209 0.512790414399177 1113 210-214 0.6096950178591243 1113 215-219 0.5104343283379862 1113 220-224 0.4690742937205812 1113 225-229 0.7780803121121949 1113 230-234 0.3889975172924274 1113 235-239 0.7886135843204158 1113 240-244 0.8755534393348299 1113 245-249 0.4330623276368186 1113 250-251 0.2563683094624487 2113 1 0.05456337763803276 2113 2 0.03680625328045295 2113 3 -0.03493711797332111 2113 4 0.10974212202457068 2113 5 0.19116085295358687 2113 6 0.12205125133520767 2113 7 0.2107202666271064 2113 8 0.1316742236172317 2113 9 0.27010464339041107 2113 10-14 0.040933788175465224 2113 15-19 0.14751416211037593 2113 20-24 0.174369897259254 2113 25-29 0.2714898454046164 2113 30-34 0.13004875449775 2113 35-39 0.10640759486732776 2113 40-44 0.19722850730693153 2113 45-49 0.12426561843964379 2113 50-54 0.1335381014749899 2113 55-59 0.1534230952622906 2113 60-64 0.06740771500275144 2113 65-69 -0.35558158992768796 2113 70-74 0.04988282092329399 2113 75-79 0.11623077417193173 2113 80-84 0.1887619507388223 2113 85-89 0.1123820263602795 2113 90-94 -0.040238641521021634 2113 95-99 -0.10722565946169738 2113 100-104 -0.19931659659098955 2113 105-109 0.20228590828504167 2113 110-114 0.17949907249754204 2113 115-119 0.21078808785996017 2113 120-124 0.22049001482550068 2113 125-129 0.2916702046592192 2113 130-134 0.12443738134389548 2113 135-139 0.16741965621164212 2113 140-144 0.25519440216688594 2113 145-149 0.18635388321585822 2113 150-154 0.1032483639320887 2113 155-159 0.22129111706395577 2113 160-164 0.10378519450884482 2113 165-169 0.005098560792774265 2113 170-174 0.04320771771131149 2113 175-179 0.24443833243210378 2113 180-184 -0.029464274347624553 2113 185-189 0.30875865225044663 2113 190-194 0.3733803113365539 2113 195-199 -6.970841371796155E-4 2113 200-204 0.29668687777356695 2113 205-209 0.15921194893078194 2113 210-214 0.35278516182525976 2113 215-219 0.309507810658765 2113 220-224 0.22190715907259317 2113 225-229 0.3411629051407701 2113 230-234 0.3350035779543141 2113 235-239 0.5479822722783894 2113 240-244 0.5335544333998925 2113 245-249 0.3025366896657147 2113 250-251 0.18754139725836083 1112 1 0.07562808070441918 1112 2 -0.00244206424468274 1112 3 -0.004048701568372337 1112 4 0.14444955250184677 1112 5 0.1808028583419663 1112 6 0.12260496280966748 1112 7 0.21049755054194463 1112 8 0.18911870331661618 1112 9 0.2604232399011934 1112 10-14 -0.052488746075638915 1112 15-19 0.1258794617123229 1112 20-24 0.04253436739945471 1112 25-29 0.20203096061204917 1112 30-34 0.1424031678584825 1112 35-39 0.07582826087173089 1112 40-44 0.21404373118134146 1112 45-49 0.25789716051555445 1112 50-54 0.1500753875249572 1112 55-59 0.12232966751736285 1112 60-64 0.11452280232943934 1112 65-69 -0.34990593521749247 1112 70-74 -0.007255788030292365 1112 75-79 0.04745587477795965 1112 80-84 0.017686134348764426 1112 85-89 0.16087743126157505 1112 90-94 0.09406416594122646 1112 95-99 0.3849838496434259 1112 100-104 0.4017521702795648 1112 105-109 0.2503911484508521 1112 110-114 0.27683550749052444 1112 115-119 0.3260419864532622 1112 120-124 0.2646452308067424 1112 125-129 0.34344677753480113 1112 130-134 0.12509236674818425 1112 135-139 0.2210690516656939 1112 140-144 0.2349023811744928 1112 145-149 0.05895525982809602 1112 150-154 0.15486085037147035 1112 155-159 0.1604803229743652 1112 160-164 0.15899577264936937 1112 165-169 -0.03830991130217143 1112 170-174 0.18144681588362488 1112 175-179 0.18515845457405078 1112 180-184 0.19628314336459596 1112 185-189 0.3281170667430757 1112 190-194 0.22334737189527942 1112 195-199 0.6613475954484613 1112 200-204 0.4360266019248904 1112 205-209 0.4379339857412319 1112 210-214 0.24160957289014817 1112 215-219 0.2025192463442238 1112 220-224 0.18211479317459833 1112 225-229 0.49175384980726733 1112 230-234 0.5614176460052036 1112 235-239 0.36980811831038807 1112 240-244 0.6189401951028515 1112 245-249 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-0.3228139140178996 2111 225-229 -0.7557851714957273 2111 230-234 -0.3902733600150263 2111 235-239 -0.17301666828864626 2111 240-244 -0.6295528931838703 2111 245-249 -0.2930222938910063 2111 250-251 0.06312442519118022 2110 1 -0.10717311669107943 2110 2 -0.11559802210605596 2110 3 -0.09133849319244547 2110 4 -0.09765969173760425 2110 5 -0.3553263749497013 2110 6 -0.29929759888997864 2110 7 -0.2523140469579843 2110 8 -0.20135856371469885 2110 9 -0.4765987159964169 2110 10-14 -0.18738332383266254 2110 15-19 -0.26199156300744164 2110 20-24 -0.12599062184844456 2110 25-29 0.0905931275362164 2110 30-34 -0.09062816930288875 2110 35-39 -0.1573071958621881 2110 40-44 -0.28756951492165683 2110 45-49 -0.24024438977874496 2110 50-54 -0.2148082911186151 2110 55-59 -0.19054846534501735 2110 60-64 -0.22317795659838424 2110 65-69 -0.19081628392098793 2110 70-74 -0.13982030957743774 2110 75-79 -0.20522988628880512 2110 80-84 -0.2689655281254204 2110 85-89 -0.21424835311502477 2110 90-94 -0.5409449631994931 2110 95-99 -0.920700376708588 2110 100-104 -1.0621523389670102 2110 105-109 -0.174398288869547 2110 110-114 -0.3106315581373664 2110 115-119 -0.04394093915949071 2110 120-124 -0.505671489454059 2110 125-129 -0.4238271195956713 2110 130-134 -0.09707041395476068 2110 135-139 -0.30855877733696957 2110 140-144 -0.23763187393657859 2110 145-149 -0.19559406584468064 2110 150-154 -0.19837443987302805 2110 155-159 -0.33622399687030224 2110 160-164 -0.3648787090030723 2110 165-169 -0.49985129098120495 2110 170-174 -0.5139133930975035 2110 175-179 -1.0866687591772362 2110 180-184 -0.5194429929672637 2110 185-189 0.033985244687961824 2110 190-194 -0.2825843082783379 2110 195-199 -0.2222823199428774 2110 200-204 -0.41651820636897696 2110 205-209 -0.22128176509808384 2110 210-214 -0.5775977092771143 2110 215-219 -0.3875235548624971 2110 220-224 -0.2782394146883007 2110 225-229 -0.17531330226262654 2110 230-234 -0.05995327172336573 2110 235-239 -0.629900268892559 2110 240-244 -0.7121772298791562 2110 245-249 -0.2780633671816446 2110 250-251 -0.037484940808337086 2109 1 -0.2108892527368127 2109 2 -0.1342155696842724 2109 3 -0.17585097533812188 2109 4 -0.22496277639605466 2109 5 -0.24590763178285613 2109 6 -0.3375995506566909 2109 7 -0.3028074323925196 2109 8 -0.2666835787862709 2109 9 -0.24843240852609227 2109 10-14 -0.20681825918050833 2109 15-19 -0.13033454852318727 2109 20-24 -0.3889449341526685 2109 25-29 -5.398016097919431 2109 30-34 -0.18623182745869116 2109 35-39 -0.11426455273623048 2109 40-44 -0.15987739998922734 2109 45-49 -0.09766245119347872 2109 50-54 -0.13644933259172376 2109 55-59 -0.2344944706875438 2109 60-64 -0.1095619183956913 2109 65-69 -0.062456257414687855 2109 70-74 -0.17861003727665548 2109 75-79 -0.16568421979854264 2109 80-84 -0.16372686863572739 2109 85-89 -0.1345508123251946 2109 90-94 -0.028697140067620808 2109 95-99 -0.06511851819820436 2109 100-104 -0.19548254315886737 2109 105-109 -0.09282207926966635 2109 110-114 -0.20349243115206406 2109 115-119 -1.2456569304739702 2109 120-124 -0.389538729160094 2109 125-129 0.011325315359400179 2109 130-134 -0.26273303041703855 2109 135-139 -0.13588051517683652 2109 140-144 -0.0835966045280756 2109 145-149 -0.09773605826318033 2109 150-154 -0.08770641996878936 2109 155-159 -0.3764491905155438 2109 160-164 -0.25532650857372374 2109 165-169 -0.208422084182601 2109 170-174 -0.37852827495063224 2109 175-179 -0.42299751888400294 2109 180-184 -0.3331493986125835 2109 185-189 -4.249008552306805 2109 190-194 -1.334810239634301 2109 195-199 -0.2759573189106419 2109 200-204 -0.3180001144316229 2109 205-209 0.06263167860997498 2109 210-214 -0.27904634124357486 2109 215-219 -0.20719081757740554 2109 220-224 -0.2288714863081509 2109 225-229 -0.3700033953948605 2109 230-234 -0.5165823998121368 2109 235-239 -0.2998533418910583 2109 240-244 -0.5125948143725481 2109 245-249 -0.13415693761608338 2109 250-251 0.07895783886001695 2108 1 -0.08277225336676963 2108 2 -0.035218387744606616 2108 3 -0.05933082146995616 2108 4 -0.1477557367601463 2108 5 -0.30522702341880503 2108 6 -0.43311678216904426 2108 7 -0.35052172403755577 2108 8 -0.2809678859001039 2108 9 -0.401069822355403 2108 10-14 0.0135562214375895 2108 15-19 -0.18968949673234903 2108 20-24 -0.3163600491827552 2108 25-29 -0.01882651368186572 2108 30-34 -0.15089954806153827 2108 35-39 -0.15498936960245402 2108 40-44 -0.26145064437847054 2108 45-49 -0.2064137701515989 2108 50-54 -0.2063637579746569 2108 55-59 -0.32030851447927233 2108 60-64 -0.2771133342828449 2108 65-69 0.004692874890238841 2108 70-74 -0.19551438932397502 2108 75-79 -0.19200673031968307 2108 80-84 -0.14149832645632898 2108 85-89 -0.16257927702720565 2108 90-94 -0.11386069255102882 2108 95-99 -0.15846401842613744 2108 100-104 -0.43332279471722046 2108 105-109 -0.044095361649361564 2108 110-114 -0.34719120703134365 2108 115-119 -0.14488484964051906 2108 120-124 -0.3832991210994834 2108 125-129 -0.3931590388623434 2108 130-134 -0.22098096522208266 2108 135-139 -0.3942153626857987 2108 140-144 -0.1826138580640091 2108 145-149 -0.1942977767984715 2108 150-154 -0.35443202515486405 2108 155-159 -0.397058286817213 2108 160-164 -0.34909086879005713 2108 165-169 -0.14925700029166222 2108 170-174 -0.19439976729399433 2108 175-179 -0.20291771476070863 2108 180-184 -0.3014136041582063 2108 185-189 0.057398903628417486 2108 190-194 -0.29420294547398385 2108 195-199 0.2007867877617926 2108 200-204 -0.22925721447365888 2108 205-209 -0.0010568789456968375 2108 210-214 -0.1461941269995961 2108 215-219 -0.23510294778384377 2108 220-224 -0.3756562380342956 2108 225-229 0.0076499966889329585 2108 230-234 -0.017149770491485583 2108 235-239 -0.3194425165459549 2108 240-244 -0.5304094692069619 2108 245-249 -0.0907706459551001 2108 250-251 -0.38963674236737234 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 2.0 13 40.0 14 122.0 15 350.0 16 673.0 17 965.0 18 2013.0 19 5775.0 20 17213.0 21 46141.0 22 86671.0 23 124425.0 24 160315.0 25 197631.0 26 246911.0 27 330261.0 28 437116.0 29 560904.0 30 754438.0 31 946073.0 32 1125801.0 33 1324196.0 34 1603940.0 35 2147667.0 36 3750736.0 37 5379701.0 38 116366.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 8.40321450822727 2.081331804503521 86.82545057570191 2.6900031115672953 2 0.7767661655628503 96.77827826540812 1.2917186767257143 1.153236892303317 3 0.7648899545120462 1.311376387799806 1.181125333992618 96.74260832369553 4 72.3141819619356 24.878033894293253 1.1399665173465487 1.6678176264245903 5 50.45256109458597 15.880957197825559 32.788070666140804 0.8784110414476668 6 13.331764640760625 84.4022388000359 1.133104132787193 1.1328924264162872 7 93.54586794087051 1.9296312808245768 1.397055505176324 3.1274452731285853 8 93.47794634079996 2.644351989002009 1.5836204536444114 2.2940812165536206 9 79.42567779343716 3.326593841740503 14.110854412833412 3.1368739519889193 10-14 32.15728825162126 32.60408164310666 12.039730077885535 23.198900027386543 15-19 38.093083263702596 2.4481879638628588 39.46939154453016 19.989337227904386 20-24 34.61382124526101 9.312454128134815 28.79157796944261 27.282146657161565 25-29 20.387252260946585 33.90668582144602 38.63296239279009 7.073099524817305 30-34 39.17996967621027 20.32408756820457 25.076446658913877 15.41949609667129 35-39 37.52560072199101 20.48741622494907 22.435612605431064 19.55137044762885 40-44 47.72835759333437 41.774543455211145 1.6154931059627564 8.881605845491732 45-49 34.098977169068604 4.233683351090851 26.922622767233594 34.74471671260695 50-54 57.761142132118614 20.4969843202 4.609810184067846 17.13206336361354 55-59 34.077746986491455 9.854938896428791 28.971077387880843 27.096236729198914 60-64 30.68481950689352 37.345122589864964 20.10201045664238 11.868047446599133 65-69 31.701811473308013 17.707864416630702 27.02427177397443 23.56605233608686 70-74 43.10843018537127 20.371042441193293 30.92531780338192 5.595209570053517 75-79 7.505143690277503 41.75772364222119 17.957896869668293 32.77923579783302 80-84 51.160794293387646 10.091654400606078 8.214328018677254 30.53322328732902 85-89 1.5904373987875733 38.40951199822621 27.07985657254821 32.920194030438005 90-94 31.587647005547637 6.1576181814670585 17.46743826926221 44.7872965437231 95-99 31.190697882210273 35.92931750027152 19.37338548718113 13.506599130337069 100-104 28.66446533349485 10.83046419565056 25.65522243988391 34.84984803097068 105-109 25.76227976986588 44.443757001155504 19.663199949025238 10.13076327995338 110-114 26.869347117173692 11.887441815602099 25.224200661288087 36.01901040593612 115-119 32.87145336703961 13.052162622426053 30.187290225447562 23.889093785086782 120-124 42.156289285086174 30.229782996838967 22.80792975644576 4.805997961629098 125-129 45.730677688616694 14.76810045580898 16.778502364347077 22.72271949122725 130-134 61.1377596075191 35.289645813176044 2.1259842926265358 1.4466102866783095 135-139 37.51107456680487 25.913140697059234 22.62163434633283 13.954150389803063 140-144 38.35817681778061 39.999619961246374 18.747072126708225 2.895131094264792 145-149 18.933534836489876 10.253984649532494 29.106208748884537 41.70627176509309 150-154 41.26436621360471 19.878503262808263 37.740518833450395 1.1166116901366414 155-159 56.795718739514726 7.922988038176958 19.70866105221371 15.572632170094606 160-164 21.77112517185652 56.635905214617075 20.278421761019032 1.3145478525073728 165-169 34.79873591675003 18.811897650193536 23.415224455741647 22.974141977314787 170-174 23.508980429346714 40.1629808587492 34.68590778091137 1.6421309309927075 175-179 41.944278696713674 26.20515634314274 12.08199286623878 19.768572093904808 180-184 34.716724325327675 45.360733150116786 2.3394122219369753 17.583130302618564 185-189 23.593332509227558 31.67327861807995 4.526280144534521 40.20710872815797 190-194 56.91926703453248 3.0484870899749708 19.620311539043474 20.411934336449075 195-199 38.43087368740759 40.8739528150906 1.3969708226279618 19.298202674873853 200-204 35.56003719009673 13.957430289481096 20.514267821777935 29.96826469864424 205-209 39.53833800521105 6.319444531221108 26.35575909339028 27.78645837017756 210-214 28.725018815791177 25.0438343209057 13.53063554903812 32.70051131426501 215-219 21.413094586378936 19.40573195515584 35.25114830051936 23.93002515794586 220-224 19.00988648097849 22.816346375581766 19.175766167938093 38.998000975501654 225-229 48.88866804873406 25.206464058572898 8.709043556149059 17.195824336543982 230-234 33.85897546715593 41.76986732619914 1.872827879725583 22.498329326919354 235-239 33.786684454132676 26.58951157068261 15.97134962191824 23.65245435326647 240-244 76.55453846879844 19.16936044093336 2.861996494989287 1.4141045952789129 245-249 23.172084575270905 53.548500881870645 1.9700723815827887 21.309342161275662 250-251 14.350353699382943 35.52449427220668 1.2483963242403897 48.87675570416998 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.6666666666666666 5 1.0 6 0.3333333333333333 7 0.3333333333333333 8 0.3333333333333333 9 0.0 10 0.6666666666666666 11 1.6666666666666665 12 1.6666666666666665 13 1.0 14 0.0 15 0.6666666666666666 16 2.6666666666666665 17 5.999999999999998 18 8.999999999999998 19 17.000000000000007 20 28.3333333333333 21 32.333333333333286 22 44.000000000000036 23 47.33333333333339 24 55.33333333333345 25 51.000000000000085 26 51.66666666666676 27 56.00000000000012 28 82.33333333333324 29 130.666666666666 30 213.33333333333502 31 587.9999999999969 32 1060.6666666667047 33 1007.6666666667113 34 1227.6666666666667 35 1529.666666666598 36 2109.9999999998417 37 2955.000000000226 38 4117.333333334059 39 8946.999999998392 40 19661.33333333333 41 26248.333333309365 42 33517.3333332911 43 50697.000000082764 44 62133.66666683264 45 76197.0000000356 46 102997.33333297893 47 124113.99999933831 48 104942.99999961728 49 102323.9999996554 50 152105.33333318253 51 312880.6666667651 52 616101.3333318147 53 801335.3333533788 54 1044531.3333816906 55 1318787.333319248 56 1288281.9999930172 57 1076231.3333757224 58 885119.3333631326 59 602677.6666635853 60 331957.3333323214 61 198482.66666786562 62 137868.99999943486 63 71280.33333344047 64 38694.6666666621 65 23250.33333332027 66 18123.333333338924 67 14892.666666675874 68 12003.333333337285 69 10340.33333333426 70 8300.666666663883 71 5400.333333332892 72 2991.0000000002424 73 1334.3333333333092 74 555.9999999999933 75 224.000000000002 76 120.66666666666603 77 81.66666666666659 78 62.3333333333335 79 55.000000000000114 80 41.000000000000014 81 30.999999999999957 82 27.999999999999968 83 20.33333333333333 84 17.33333333333334 85 16.666666666666675 86 10.666666666666668 87 8.666666666666664 88 17.66666666666667 89 22.666666666666654 90 21.99999999999999 91 15.666666666666677 92 8.33333333333333 93 5.999999999999998 94 8.33333333333333 95 9.666666666666666 96 10.333333333333334 97 13.000000000000005 98 9.666666666666666 99 16.333333333333343 100 59.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 1.032714004417744E-6 15-19 0.0 20-24 0.0 25-29 0.0 30-34 6.196284026506464E-6 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 1.032714004417744E-6 60-64 0.0 65-69 0.0 70-74 5.20487858226543E-4 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 1.032714004417744E-6 100-104 0.0 105-109 0.0 110-114 0.0 115-119 1.032714004417744E-6 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-154 0.0 155-159 0.0 160-164 0.0 165-169 0.0 170-174 0.0 175-179 1.032714004417744E-6 180-184 1.032714004417744E-6 185-189 0.0 190-194 1.032714004417744E-6 195-199 0.0 200-204 0.0 205-209 1.032714004417744E-6 210-214 1.032714004417744E-6 215-219 0.0 220-224 0.0 225-229 1.032714004417744E-6 230-234 0.0 235-239 0.0 240-244 1.032714004417744E-6 245-249 1.032714004417744E-6 250-251 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 251 1.9366446E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 3.7645575685868744 #Duplication Level Percentage of deduplicated Percentage of total 1 71.53949348787934 2.6931454166266753 2 10.52226151725994 0.7922331846690263 3 3.9302003334798297 0.4438639623439245 4 2.410778968048711 0.36302064841431314 5 1.5991379043640848 0.30100233505439844 6 1.1296132200064561 0.2551496398170657 7 0.7835898457245088 0.20649083590730147 8 0.6501831417969165 0.19581214939353397 9 0.4660134930504216 0.15789011600939143 >10 4.707214860315986 3.8260512326071887 >50 1.1913285766308512 3.1392632064531445 >100 0.794719651650663 6.145634102883691 >500 0.11458800489196076 2.9730307207941755 >1k 0.11126275336457744 9.175525977811517 >5k 0.02042951998583393 5.423049100969771 >10k+ 0.029184721550104695 63.908837370245095 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TACGGGGGGGGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCTCGTA 557869 2.8805956446526118 No Hit TACAGAGGGGGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGTA 476156 2.4586648474376767 No Hit TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA 451960 2.333727107183218 No Hit TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGTGTGCGCA 368805 1.90435044199643 No Hit TACGGGGGGAGCAAGCGTTGTTCGGATTTACTGGGCGTAAAGGGCGCGTA 332511 1.7169438316147423 No Hit TACGAAGGGGGCTAGCGTTGCTCGGAATCACTGGGCGTAAAGCGCACGTA 330091 1.7044479921612878 No Hit TACGGAGGGTGCGAGCGTTGTCCGGAATCACTGGGCGTAAAGGGCGCGTA 299325 1.545585596861706 No Hit TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA 274290 1.416315621358715 No Hit TACAGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGTA 245974 1.2701039726132508 No Hit TACAGAGGTGGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGTA 237766 1.2277213898719466 No Hit TACGTAGGGGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGAGCGTGTA 196923 1.0168256994597769 No Hit TACGGAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTA 194556 1.004603529217493 No Hit TACGGAGGGTGCAAGCGTTATCCGGATTCACTGGGTTTAAAGGGTGCGTA 192742 0.9952368131974241 No Hit TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGCGTAAAGAGCGCGCA 176338 0.9105336105550806 No Hit TACAGAGGTCTCAAGCGTTGTTCGGATTCATTGGGCGTAAAGGGTGCGTA 164806 0.8509873210603536 No Hit TACGGGGGGGGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGTA 161194 0.8323365061405691 No Hit TACGTAGGGTGCAGGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA 154432 0.7974204456512053 No Hit TACAGAGGTCCCAAGCGTTGTTCGGATTCACTGGGCGTAAAGGGTGCGTA 151926 0.7844805391758509 No Hit TACGGAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGCGTA 144614 0.7467245151743381 No Hit GACAGAGGGGGCAAGCGTTGCTCGGAATCACTGGGCGTAAAGGGCGCGTA 137563 0.7103161829485906 No Hit TACGAAGGGTGCAAGCGTTACTCGGAATTACTGGGCGTAAAGCGTGCGTA 133884 0.6913194088373262 No Hit TACAGAGGTCCCGAGCGTTGTTCGGATTCACTGGGCGTAAAGGGTGCGTA 132571 0.6845396413983237 No Hit TACAGAGGGTGCAAGCGTTGTTCGGAATCATTGGGCGTAAAGGGCGTGTA 131406 0.6785240823225903 No Hit TACAGAGGGTGCGAGCGTTGTCCGGAATCACTGGGCGTAAAGGGCGCGTA 127673 0.6592484754301331 No Hit TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA 120827 0.6238986750589138 No Hit TACGTAGGGACCAAGCGTTGTTCGGATTTACTGGGCGTAAAGGGCGCGTA 119773 0.6184562722556323 No Hit TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGTTTAAAGGGTGCGTA 118140 0.6100241624095614 No Hit TACGAAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTA 114607 0.5917812695215219 No Hit TACAGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGTA 114448 0.5909602618880098 No Hit TACCAGCACCCCGAGTGGTCGGGACGATTATTGGGCCTAAAGCATCCGTA 105283 0.5436361426355667 No Hit TACGTAGGGGGCAAGCGTTGTCCGGAATCATTGGGCGTAAAGAGCGTGTA 104432 0.5392419445467692 No Hit TACGTAGGGGGCTAGCGTTGTCCGGAATCATTGGGCGTAAAGCGCGCGTA 103649 0.5351988692194738 No Hit TACAGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA 101553 0.5243760264531758 No Hit TACGAAGGGAGCTAGCGTTGTTCGGAATCACTGGGCGTAAAGCGCACGTA 100091 0.516826887080882 No Hit TACGGAGGGAGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTA 98738 0.509840576840996 No Hit TACGTAGGGGGCTAGCGTTGTCCGGAATCATTGGGCGTAAAGCGCGTGTA 94844 0.4897336351749826 No Hit TACGAAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGTA 81943 0.42311841832001595 No Hit TACGAAGGGGGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGTA 78548 0.4055880980950248 No Hit TACGGGGGGGGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGTTCGTA 78070 0.4031199116244663 No Hit GACAGAGGGTGCAAACGTTGTTCGGAATTACTGGGCGTAAAGCGCGTGTA 77936 0.40242799324150647 No Hit TACGGAGGGGACTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTA 76502 0.39502343382983124 No Hit TACGTAGGGGGCAAGCGTTGTCCGGATTCATTGGGCGTAAAGAGCGTGTA 76469 0.3948530360191023 No Hit TACGTAGGGAGCAAGCGTTGTTCGGATTTACTGGGCGTAAAGGGCGCGTA 74390 0.38411797394317987 No Hit TACGTAGGGTGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA 70731 0.36522447123235724 No Hit TACGAAGGGGGCTAGCGTTGTTCGGATTTACTGGGCGTAAAGCGCACGTA 70640 0.36475458636034713 No Hit TACGGAGGGAGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGCGTA 69570 0.3592295664367122 No Hit TACAGAGGGGGCAAGCGTTATTCGGAATTATTGGGCGTAAAGGGCGCGTA 69425 0.3584808487835094 No Hit TACGTAGGTGGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGGGCGCGTA 69196 0.3572983912484511 No Hit TACAGAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGTGCGTA 69008 0.35632764008429835 No Hit TACGTAGGGGGCAAGCGTTGTCCGGAATCATTGGGCGTAAAGCGCGCGTA 65916 0.34036188157600006 No Hit TACAGAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGTA 65445 0.3379298400955963 No Hit TACGAAGGTGGCAAGCGTTGTTCGGATTTACTGGGCGTACAGGGAGCGTA 63732 0.3290846446477583 No Hit TACGTAGGGGGCAAGCGTTGTCCGGAATCATTGGGCGTAAAGCGCGTGTA 63460 0.3276801536017501 No Hit TACGTAGGGGGCTAGCGTTGTCCGGATTTATTGGGCGTAAAGAGCGTGTA 62289 0.3216336131058843 No Hit TACGAAGGGGGCTAGCGTTGTTCGGAATCACTGGGCGTAAAGCGCACGTA 61712 0.31865423320313907 No Hit TACGAAGGGGGCTAGCGTTGCTCGGAATCACTGGGCGTAAAGGGTGCGTA 59187 0.30561621889736507 No Hit TACGTAGGTGGCGAGCGTTGTCCGGATTTACTGGGCGTAAAGAGCGCGCA 56071 0.2895265347085366 No Hit TACGTAGGTGACAAGCGTTGTCCGGATTTACTGGGCGTAAAGAGCGCGCA 55290 0.2854937865212853 No Hit TACGAAGGGGGCTAGCGTTGTTCGGAATCACTGGGCGTAAAGGGCGCGTA 53910 0.2783680598908029 No Hit TACGTAGGGGGCAAGCGTTGTCCGGATTTACTGGGCGTAAAGAGCGTGTA 53819 0.2778981750187928 No Hit GACAGAGGGGGCAAGCGTTGCTCGGAATTACTGGGCGTAAAGGGCGCGTA 51458 0.26570698619664135 No Hit TACGAAGGGGGCTAGCGTTGCTCGGAATTACTGGGCGTAAAGCGCACGTA 50537 0.2609513382062976 No Hit CACGGGGGGAGCAAGCGTTGTTCGGAATCACTGGGCGTAAAGGGCGTGTA 50378 0.26013033057278556 No Hit GACGAACCGTGCGAACGTTGTTCGGAATCACTGGGCTTAAAGGGCGCGTA 49561 0.25591169386473905 No Hit TACGGAGGGTGCAAACGTTGCTCGGAATCATTGGGCGTAAAGCGCACGTA 48199 0.24887891149465421 No Hit TACGTAGGGCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA 48105 0.24839353591257787 No Hit TACGAAGGGGGCTAGCGTTGCTCGGAATTACTGGGCGTAAAGGGCGCGTA 47851 0.24708198912696736 No Hit TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGGGTGCGCA 47746 0.24653981427464802 No Hit TACGAAGGGGGCTAGCGTTGTTCGGATTTACTGGGCGTAAAGCGCGTGTA 47723 0.24642105216413998 No Hit TACGGGGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCTCGTA 46591 0.24057589089913553 No Hit TACGTAGGGTGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA 45789 0.23643470774142042 No Hit TACGTAGGGTGCGAGCGTTGTCCGGAATTACTGGGCGTAAAGAGCTCGTA 45769 0.2363314363409786 No Hit TACGTAGGGGGCTAGCGTTGTCCGGAATCATTGGGCGTAAAGAGCGTGTA 45628 0.23560337296786413 No Hit TACGGGGGGGGTAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCTCGTA 44911 0.23190109326202651 No Hit TACGAAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGCGCA 44059 0.2275017316032069 No Hit CACGTAGGGGACGAGCGTTGTCCGGATTCATTGGGCGTAAAGCGCGCGTA 43981 0.227098973141484 No Hit TACGAAGGGTGCAAACGTTGCTCGGAATTATTGGGCGTAAAGCGCACGTA 43856 0.22645352688872292 No Hit TACAGAGGGTGCAAGCGTTGTTCGGAATTATTGGGCGTAAAGCGCGTGTA 43470 0.22446038886019667 No Hit GACGGAGGGTGCAAACGTTGTTCGGAATCACTGGGCGTAAAGGGCGCGTA 41840 0.21604376972419204 No Hit GACAGAGGGTGCAAACGTTGCTCGGAATTACTGGGCGTAAAGCGCGTGTA 41495 0.21426233806657144 No Hit TACAGAGGGCGCGAGCGTTGTCCGGAATCACTGGGCGTAAAGGGCGCGTA 41390 0.2137201632142521 No Hit TACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA 41179 0.2126306499395914 No Hit TACAGAGACTGCAAGCGTTATTCGGATTCACTGGGCGTAAAGGGTGCGCA 41115 0.21230018145817772 No Hit TACGGAGGGTGCGAGCGTTATCCGGAATTACTGGGTTTAAAGGGTGCGTA 41079 0.21211429293738251 No Hit TACGTAGGGGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGTGTA 39536 0.20414690439329963 No Hit TACGTAGGGAGCAAGCGTTGTCCGGAATCATTGGGCGTAAAGCGCGCGTA 38884 0.20078025673889777 No Hit TACGTAGGGTGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTCGTA 37657 0.19444455632179491 No Hit TACGTAGGGCGCAAGCGTTATCCGGAATTATTGGGCGTAAAGAGCTCGTA 37509 0.1936803479585258 No Hit GACGTAGGGCGCGAGCGTTGTCCGGATTTATTGGGCGTAAAGAGCTCGTA 36694 0.18947203839052348 No Hit TACGGGGGGGGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGTGCGTA 36588 0.1889246999681821 No Hit TACGTAGGGGGCGAGCGTTGTCCGGATTTATTGGGCGTAAAGAGCTCGTA 35535 0.18348746073492267 No Hit TACGTAGGGGGCAAGCGTTGTCCGGATTTACTGGGCGTAAAGCGCGTGTA 35530 0.18346164288481223 No Hit TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGTA 35174 0.18162341195694864 No Hit TACGAAGGGGGCTAGCGTTGCTCGGAATCACTGGGCGTAAAGGGCGCGTA 34447 0.17786949655089013 No Hit TACAGGGGGGGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCTCGTA 33667 0.17384191193366094 No Hit TACGTAGGGTGCGGGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA 33499 0.17297443216995 No Hit TACAGAGGGGGCGAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGTA 32490 0.1677643900176625 No Hit TACGAAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGCGTA 31504 0.16267310997588302 No Hit TACGTAGGGGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCGTGTA 31260 0.16141319889049338 No Hit TACGTAGGGGGCTAGCGTTGTCCGGAATCATTGGGCGTAAAGAGCGCGTA 31115 0.16066448123729052 No Hit TACGAAGGGGACTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTA 30387 0.15690540226120994 No Hit TACGTAGGTGGCGAGCGTTGTCCGGATTTACTGGGCGTAAAGCGCGCGCA 30335 0.1566368966200613 No Hit TACGTAGGGGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGAGCTCGTA 30230 0.156094721767742 No Hit GACGTAGGGAGCGAGCGTTGTCCGGATTCATTGGGCGTAAAGCGCGCGTA 30062 0.1552272420040311 No Hit TACGGAGGGTGCGAGCGTTGTTCGGAATCACTGGGCGTAAAGGGCGTGTA 30009 0.1549535727928604 No Hit TACGGAGGTGGCAAGCGTTGCTCGGAATCACTGGGCGTAAAGGGCGCGTA 29327 0.15143201803779588 No Hit TACGTAGGGTCCGAGCGTTGTCCGGAATTACTGGGCGTAAAGAGCTCGTA 29041 0.1499552370114785 No Hit TACGGAGGGGGCGAGCGTTATTCGGAATTATTGGGCGTAAAGGGCGCGTA 28530 0.14731665273019118 No Hit TACGGAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGTACGTA 28337 0.14632008371592806 No Hit TACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGTA 28145 0.14532867827168702 No Hit TACAGGGGGGGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGTTCGTA 28107 0.14513246261084764 No Hit TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA 27480 0.14189490420699802 No Hit TACAGAGGGGGCCAGCGTTATTCGGAATTATTGGGCGTAAAGGGCGCGTA 26551 0.1370979476564776 No Hit TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGCGTAAAGGGCGTGCA 26254 0.13556436735991725 No Hit TACGGAGGGTGCAAGCGTTGTTCGGAATCACTGGGCGTAAAGGGCGTGTA 25517 0.13175881625363786 No Hit TACAGAGGGTGCTAGCGTTGTTCGGAATCATTGGGCGTAAAGGGCGTGTA 25081 0.12950749972400719 No Hit TACGAAGGGTGCAAACGTTGCTCGGAATCATTGGGCGTAAAGCGCACGTA 24940 0.12877943635089267 No Hit TACGTAGGGGGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGTA 24849 0.12830955147888262 No Hit TACGGAGGGTGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTCCGTA 24447 0.12623379633000292 No Hit CACGTAGGGTGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA 23872 0.12326474356730192 No Hit TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGTGTGCGCA 22814 0.11780168648393206 No Hit CACGTAGGGGGCGAGCGTTGTCCGGATTTATTGGGCGTAAAGAGCTCGTA 22642 0.1169135524401328 No Hit TACGTAGGGGGCAAGCGTTGTCCGGAATCATTGGGCGTAAAGAGCGCGTA 22588 0.11663471965894 No Hit TACGGAGGGGGCCAGCGTTGTTCGGAATCATTGGGCGTAAAGGGCGCGTA 22585 0.11661922894887373 No Hit TACGGAGGGAGCTAGCGTTGTTCGGAAATACTGGGCGTAAAGCGCACGTA 22109 0.1141613696183595 No Hit TACAGAGGTCCCAAGCGTTGTTCGGATTTACTGGGCGTAAAGGGTGCGTA 21354 0.11026287425168252 No Hit TACGGAGGGAGCTAGCGTTGTTCGGATATACTGGGCGTAAAGCGCACGTA 20709 0.1069323715874353 No Hit GACAGAGGTGCCAAGCGTTAGGCGGAATCACTGGGCTTAAAGCGTGTGTA 19758 0.10202181649642893 No Hit TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA 19417 0.10026103911889668 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 3.3046848141367807E-4 0.0 0.0 2 3.3046848141367807E-4 0.0 0.0 3 3.3563205143576677E-4 0.0 0.0 4 3.407956214578555E-4 0.0 0.0 5 3.5112276150203297E-4 0.0 0.0 6 3.5628633152412167E-4 0.0 0.0 7 3.6144990154621037E-4 0.0 0.0 8 3.666134715682991E-4 0.0 0.0 9 3.666134715682991E-4 0.0 0.0 10-14 3.944967496875782E-4 0.0 0.0 15-19 4.213473138024395E-4 0.0 0.0 20-24 4.99833578138188E-4 0.0 0.0 25-29 5.876142685136963E-4 0.0 0.0 30-34 7.66273791277966E-4 0.0 0.0 35-39 9.025920398611081E-4 0.0 0.0 40-44 0.0010275504343956554 0.0 0.0 45-49 0.00121756981120852 0.0 0.0 50-54 0.0014581921742378545 0.0 0.0 55-59 0.0017876279416471148 0.0 0.0 60-64 0.002174895693303769 0.0 0.0 65-69 0.0025941775790973725 0.0 0.0 70-74 0.003160104853518296 1.2392568053012929E-5 0.0 75-79 0.00383136895638983 1.549071006626616E-5 0.0 80-84 0.004759778846361381 1.549071006626616E-5 0.0 85-89 0.005953596235468294 1.549071006626616E-5 0.0 90-94 0.007422115549750326 1.549071006626616E-5 0.0 95-99 0.00931508031984805 1.549071006626616E-5 4.130856017670976E-6 100-104 0.01149823772518716 1.549071006626616E-5 1.4457996061848418E-5 105-109 0.014339233951340374 1.549071006626616E-5 1.549071006626616E-5 110-114 0.017695554465698045 1.549071006626616E-5 1.549071006626616E-5 115-119 0.021822279627351348 1.549071006626616E-5 1.549071006626616E-5 120-124 0.02986815443576999 1.549071006626616E-5 1.549071006626616E-5 125-129 0.037361527251825136 1.549071006626616E-5 2.7883278119279093E-5 130-134 0.043062108556211086 1.549071006626616E-5 3.098142013253232E-5 135-139 0.04921295316652317 0.004718470286184672 3.098142013253232E-5 140-144 0.055531097445550924 0.015599145036730022 3.098142013253232E-5 145-149 0.062415168998999605 0.015603275892747692 3.098142013253232E-5 150-154 0.06953160120344229 0.01560430860675211 3.098142013253232E-5 155-159 0.07668314568403516 0.01560430860675211 3.098142013253232E-5 160-164 0.08407531252765738 0.01560430860675211 3.098142013253232E-5 165-169 0.09193839695729408 0.01560430860675211 3.098142013253232E-5 170-174 0.09965999956832555 0.01560430860675211 3.098142013253232E-5 175-179 0.10727213449488873 0.01560430860675211 3.098142013253232E-5 180-184 0.11490285827353144 0.01560430860675211 3.098142013253232E-5 185-189 0.1230241212042726 0.01560430860675211 3.098142013253232E-5 190-194 0.13114848227702697 0.01560430860675211 3.098142013253232E-5 195-199 0.13929969391389624 0.01560430860675211 3.098142013253232E-5 200-204 0.14819239420593744 0.01560430860675211 3.098142013253232E-5 205-209 0.1568640937010332 0.01560430860675211 3.098142013253232E-5 210-214 0.1657444014250214 0.01560430860675211 3.098142013253232E-5 215-219 0.17463297086104493 0.01560430860675211 3.717770415903878E-5 220-224 0.18387163034456605 0.01560430860675211 4.130856017670976E-5 225-229 0.19306794855390605 0.01560430860675211 4.130856017670976E-5 230-234 0.20225703776521514 0.01560430860675211 4.130856017670976E-5 235-239 0.21179724973802627 0.01560430860675211 4.130856017670976E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCAAACA 835185 0.0 243.15758 245 GTGCGAG 287760 0.0 243.00325 9 CGTAGGG 477270 0.0 242.95415 3 GGTCCCA 28110 0.0 242.21075 7 TACGAAG 219720 0.0 241.95569 1 TACAGAG 375030 0.0 241.94897 1 GCGAACA 173600 0.0 241.40807 245 ACGGAGG 298225 0.0 240.30888 2 GTAGGGT 231725 0.0 239.81381 4 AGGGGGC 523260 0.0 239.72064 6 GGGTGCA 288945 0.0 239.64525 7 AGGTCCC 50435 0.0 239.60771 6 TACGGAG 287380 0.0 239.59476 1 CGAAGGG 209020 0.0 239.18013 3 TACGTAG 553325 0.0 239.04222 1 TCAAACA 62265 0.0 238.97957 245 ACAGAGG 440700 0.0 237.64201 2 ACGGGGG 199045 0.0 237.51605 2 TACGGGG 188440 0.0 237.44594 1 GAACCGT 16140 0.0 237.03047 4 >>END_MODULE