FastQCFastQC Report
Thu 5 May 2016
000000000-AHW8P_l01n01.331000000544c6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AHW8P_l01n01.331000000544c6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18784682
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCGTCCACGAGGTCTCTTGTATGGCACTCTCGCGCATCGTACGCTGCA522260.2780244030748032No Hit
ATGCGGTGTCGGTCTCGTAGCCTATCGGGTAGTAGAGCTTATTGATGAATT446500.23769366976773948No Hit
ATGCGGTCCACGAGGTCTCTTGTATGGCACTCTCGCGCATCGTACGCTGCA406540.21642101793365467No Hit
GGAACGTCCACGAGGTCTCTTGTATGGCACTCTCGCGCATCGTACGCTGCA384260.20456029013427002No Hit
ATGCGGTCCACGAGGTCTCTCCCACTACGGTAACTGATAGCGTACGCTGCA306820.163335211104452No Hit
AGACAGTCCACGAGGTCTCTTGTATGGCACTCTCGCGCATCGTACGCTGCA300680.1600665904272428No Hit
CTCAGGTCCACGAGGTCTCTTGTATGGCACTCTCGCGCATCGTACGCTGCA261320.13911334777985596No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTAAA1050.045.035
GGGGAAC1900.045.020
GTGTGGC2250.045.020
GGAAATT4750.045.028
CGTGGGG1750.044.99999616
AACCCCG2350.044.99999636
GTGGGGC600.044.99999617
AAACCGC453.8562575E-1044.99999629
CGGGCCT453.8562575E-1044.99999621
TGGGGCG207.036057E-444.99999618
GGGCCGG551.8189894E-1244.99999628
ACATGTG193550.044.8953742
GTGTCGG7481750.044.8950426
TGTCGGT7561900.044.8854457
TTCGTGT281550.044.8561483
TCGGTCT7568600.044.8531389
AAACCCA13800.044.83695230
GTCGGTC7570250.044.8353428
CGGTCTC7573550.044.81461310
CTCCGGG22050.044.79591419
Produced by FastQC (version 0.11.2)