##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AHV8M l01n03.33300000033c8a.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	19291995
Sequences flagged as poor quality	0
Sequence length	301
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.428894834360054	27.0	21.0	32.0	12.0	34.0
2	26.193645032564024	31.0	21.0	34.0	11.0	34.0
3	27.57142498741058	32.0	23.0	34.0	11.0	34.0
4	27.733163832978395	32.0	23.0	34.0	11.0	34.0
5	25.49763614390321	30.0	19.0	34.0	11.0	34.0
6	30.024798368442454	34.0	26.0	37.0	11.0	38.0
7	28.765580490768322	32.0	25.0	37.0	11.0	37.0
8	28.676594359473967	32.0	23.0	37.0	11.0	38.0
9	28.960102000855795	33.0	25.0	37.0	11.0	38.0
10-14	30.829894917555187	35.2	27.8	37.2	11.0	38.0
15-19	31.082814949931304	36.0	27.6	37.4	11.0	38.0
20-24	30.898493401019437	35.4	28.0	37.4	11.0	38.0
25-29	29.634309567258338	33.4	24.8	36.8	11.0	37.6
30-34	28.71590472628673	32.6	23.0	36.8	11.0	37.8
35-39	26.52237022661472	29.2	19.4	35.6	10.4	37.0
40-44	26.950173364652024	30.0	17.0	36.4	11.0	37.2
45-49	27.043238026964037	30.2	18.6	36.4	10.6	37.4
50-54	26.84744865422161	29.2	18.4	35.8	10.8	37.2
55-59	29.594101221776185	33.8	24.4	37.0	11.0	38.0
60-64	29.69215025195684	33.8	24.6	37.0	11.0	38.0
65-69	26.489716838512557	29.2	17.4	35.8	10.8	37.4
70-74	24.794107327935755	26.2	11.0	35.4	10.8	37.0
75-79	27.301812850355805	31.2	20.8	36.2	11.0	37.2
80-84	25.79243866691859	28.2	15.4	35.4	10.8	37.2
85-89	26.63955660365867	29.6	19.0	36.2	11.0	37.4
90-94	25.299976969722415	27.8	14.6	34.6	10.6	37.2
95-99	26.698331375267305	29.4	19.0	35.8	11.0	37.0
100-104	25.809528729403052	27.8	14.0	35.8	10.8	37.0
105-109	25.11643982905863	27.4	15.0	35.0	10.6	37.0
110-114	24.78168414412299	26.2	12.6	34.4	11.0	37.4
115-119	22.007862421693556	22.0	11.0	31.2	10.4	36.2
120-124	23.19254201548362	24.0	11.0	33.0	11.0	37.0
125-129	25.454888361727235	28.0	13.4	35.4	11.0	37.0
130-134	24.41165866982653	25.8	11.0	34.8	10.0	37.0
135-139	22.677066793766016	22.6	11.0	32.4	10.6	36.6
140-144	21.130160286688856	20.4	10.4	30.2	9.8	36.2
145-149	20.20326330169586	18.8	10.4	28.4	9.6	35.0
150-154	20.680921791655038	19.2	10.4	29.6	10.2	35.4
155-159	21.05827357927472	20.2	10.6	30.2	10.2	36.0
160-164	19.438663155365738	18.0	9.8	28.2	9.4	35.0
165-169	17.68680879297346	15.2	9.6	24.8	8.8	33.8
170-174	17.61327249981145	15.6	9.6	24.6	8.6	33.2
175-179	19.037120038648155	17.4	10.4	26.6	9.4	34.2
180-184	19.835510013350095	18.4	10.6	28.6	10.0	35.0
185-189	18.526309031284736	16.8	10.6	25.4	9.6	33.4
190-194	18.75315420722429	16.2	10.4	26.2	10.2	33.8
195-199	15.846236897739193	13.8	9.0	21.2	8.2	28.8
200-204	17.418495235977407	15.6	9.8	24.2	8.8	31.0
205-209	18.829562551721583	17.8	10.4	26.4	9.6	33.6
210-214	16.494686620020378	13.6	9.8	22.0	8.8	29.6
215-219	15.05982572564424	12.2	9.0	19.6	8.0	26.8
220-224	14.577332743451365	10.8	8.8	18.6	7.8	26.2
225-229	14.31543747549178	11.0	8.4	18.2	7.6	25.6
230-234	13.942459854462953	10.6	8.2	17.6	7.4	25.0
235-239	13.763851960359725	10.0	8.4	17.0	7.4	24.8
240-244	14.199885403246267	10.6	8.6	17.6	8.0	25.0
245-249	12.27821100928131	9.0	8.0	15.0	7.0	21.4
250-254	12.077398506478982	9.0	8.0	15.0	7.0	20.2
255-259	12.005329163728272	9.2	8.0	14.6	7.0	20.6
260-264	12.015174511500755	9.0	8.0	14.6	7.0	20.8
265-269	11.747210353309754	9.2	7.2	14.2	7.0	20.0
270-274	12.397863372865274	9.6	8.0	15.4	7.0	21.4
275-279	12.603767013209367	9.6	8.2	15.6	7.2	21.8
280-284	12.080087414494974	9.4	7.6	14.6	7.0	20.8
285-289	11.39024453406711	8.8	7.0	13.8	7.0	19.0
290-294	11.462786974597496	8.8	7.4	13.8	7.0	19.4
295-299	11.477606333611428	9.0	7.8	13.8	7.0	19.0
300-301	11.477218426606477	9.5	8.0	14.0	7.0	19.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	1.6278490369449372
1101	2	2.860785615571963
1101	3	2.8975098850101944
1101	4	2.908024635646001
1101	5	4.011940678628779
1101	6	2.7012578298171697
1101	7	2.5648712097399873
1101	8	3.2513819868731026
1101	9	1.5043867651168803
1101	10-14	0.20308415653500234
1101	15-19	2.0154250297321816
1101	20-24	2.2881864198795263
1101	25-29	1.8186938178772998
1101	30-34	2.6087131808197626
1101	35-39	3.4592905072424074
1101	40-44	2.9884542931307045
1101	45-49	3.0575171113909043
1101	50-54	3.2814895077498463
1101	55-59	0.8011038929988956
1101	60-64	0.774179315867805
1101	65-69	2.352825916364157
1101	70-74	3.0766613383304104
1101	75-79	3.1010053162857396
1101	80-84	2.5811415750289264
1101	85-89	4.199104783114521
1101	90-94	3.5820386035789085
1101	95-99	2.7842242961319066
1101	100-104	3.0048505087541386
1101	105-109	2.5075828752579383
1101	110-114	2.4841495946006127
1101	115-119	2.4713137573581356
1101	120-124	2.465852942708846
1101	125-129	1.7793982349144812
1101	130-134	1.0563089892918818
1101	135-139	2.1263269065451595
1101	140-144	1.6305054847546394
1101	145-149	2.2179473214838836
1101	150-154	2.075959794663575
1101	155-159	2.7496216451815627
1101	160-164	1.8589757459672391
1101	165-169	1.3583099634316227
1101	170-174	1.6998266974988567
1101	175-179	0.8146529164198526
1101	180-184	0.6702966786281763
1101	185-189	1.0989395248929306
1101	190-194	1.730676205671056
1101	195-199	0.8289139466800677
1101	200-204	1.6909471401962648
1101	205-209	2.2332144117969257
1101	210-214	1.4521020174330772
1101	215-219	1.8540481627376213
1101	220-224	1.0206580771946143
1101	225-229	0.655506482282064
1101	230-234	1.1806468621549087
1101	235-239	0.6027040177651575
1101	240-244	0.8508064657723011
1101	245-249	0.2082275699622933
1101	250-254	0.710818388820087
1101	255-259	-0.006156307980713649
1101	260-264	0.6835659410122616
1101	265-269	1.1424677935713987
1101	270-274	1.640473351429149
1101	275-279	0.5587670804160378
1101	280-284	0.6101324000092294
1101	285-289	2.015515348867231
1101	290-294	2.759370485149642
1101	295-299	2.8572160204558674
1101	300-301	2.968253066276686
1102	1	0.4495872426506651
1102	2	1.3793092605404063
1102	3	1.8418456172497848
1102	4	1.927679915953611
1102	5	1.707037269226241
1102	6	0.9340991444706219
1102	7	1.29611694260144
1102	8	0.4538424533316423
1102	9	-1.079821080782569
1102	10-14	-0.025686435511190098
1102	15-19	1.1358253044829887
1102	20-24	0.8871859620536817
1102	25-29	0.43254486790318225
1102	30-34	1.1651921631845426
1102	35-39	0.6002526443354839
1102	40-44	1.2055493072779555
1102	45-49	1.455889104247472
1102	50-54	1.1284513534923448
1102	55-59	0.7862135928643177
1102	60-64	0.5054317970984314
1102	65-69	1.214761778717385
1102	70-74	1.3575190076021784
1102	75-79	1.0467605537466405
1102	80-84	0.6943904473179394
1102	85-89	2.4542853689456052
1102	90-94	1.9409053033613333
1102	95-99	1.7911290107930888
1102	100-104	2.0323711204692074
1102	105-109	0.7855371553113244
1102	110-114	1.0487001059584564
1102	115-119	1.1790191117589295
1102	120-124	0.9624761094259782
1102	125-129	0.5467864144562213
1102	130-134	-0.0170315534873815
1102	135-139	0.8825780995592964
1102	140-144	0.0011506042139046713
1102	145-149	1.0866668463665192
1102	150-154	0.7253420049997388
1102	155-159	1.4615612088006813
1102	160-164	0.8632695248292599
1102	165-169	0.044317379888706654
1102	170-174	0.2982385967884298
1102	175-179	-0.26114318314216334
1102	180-184	-0.4291385402452086
1102	185-189	0.40777377732997167
1102	190-194	0.7560811430114533
1102	195-199	0.08168573484468844
1102	200-204	0.8856883106096873
1102	205-209	1.5659971880082644
1102	210-214	0.646454456897839
1102	215-219	0.27051008760803263
1102	220-224	0.3343981747059388
1102	225-229	0.3197828190699621
1102	230-234	0.07423197390146541
1102	235-239	0.3203782134140969
1102	240-244	0.4099458150020343
1102	245-249	0.24204997017930552
1102	250-254	0.12673150896184815
1102	255-259	0.4793638277100811
1102	260-264	-0.010363923857473978
1102	265-269	0.0656376017937923
1102	270-274	-0.7393240999683925
1102	275-279	-0.5291061919348454
1102	280-284	-0.7487875351621884
1102	285-289	-0.30865095198784864
1102	290-294	-0.6556738900749188
1102	295-299	-0.833434249481412
1102	300-301	-0.6478389214667306
1103	1	0.2899650130230498
1103	2	0.9115417219540483
1103	3	1.134230898066157
1103	4	0.9757861408922892
1103	5	1.0739426734158464
1103	6	1.2442696889447191
1103	7	-0.0163551129210866
1103	8	1.0878896346273876
1103	9	0.9187350872955662
1103	10-14	0.5654439918446386
1103	15-19	0.30171802473369524
1103	20-24	-0.08970316043663118
1103	25-29	0.22707511663060842
1103	30-34	0.09280859090012683
1103	35-39	-0.31284392081681034
1103	40-44	0.6341167687933336
1103	45-49	0.8166184845181768
1103	50-54	0.3598022247833619
1103	55-59	0.3681547493931703
1103	60-64	0.8903866653574646
1103	65-69	0.3318473132806474
1103	70-74	0.548671311425224
1103	75-79	0.39001470794819326
1103	80-84	0.2705288553834997
1103	85-89	1.5424365410635268
1103	90-94	1.0192128019274982
1103	95-99	1.12399263848679
1103	100-104	1.551297099524696
1103	105-109	0.24304961363128896
1103	110-114	0.6360520248516295
1103	115-119	0.7502119691980269
1103	120-124	0.6463582363062805
1103	125-129	0.24993562866636765
1103	130-134	-0.09112533960004043
1103	135-139	0.42693825104855065
1103	140-144	-0.2379492079942338
1103	145-149	0.46070954306105705
1103	150-154	0.25405744275733255
1103	155-159	0.5540092465050606
1103	160-164	0.3507763773821786
1103	165-169	0.4207516969393197
1103	170-174	-0.17173821237906495
1103	175-179	-0.4116098648488524
1103	180-184	-0.06415922308901756
1103	185-189	0.3077449944016557
1103	190-194	0.9963536290015078
1103	195-199	0.11221126880939458
1103	200-204	0.33227222481426466
1103	205-209	-0.1528087541557639
1103	210-214	0.44537883399489075
1103	215-219	0.0066167459001125195
1103	220-224	0.520598154735989
1103	225-229	0.27584803449406614
1103	230-234	-0.021299329201587724
1103	235-239	0.46772120355928415
1103	240-244	0.3320363691469179
1103	245-249	0.3656701255569068
1103	250-254	0.2549470143858539
1103	255-259	0.5329076976392351
1103	260-264	0.06365882152879365
1103	265-269	0.12066332025186277
1103	270-274	0.023204719468338908
1103	275-279	1.3470941139056745
1103	280-284	-0.6679176848427364
1103	285-289	-0.314646654757178
1103	290-294	-0.3216656130845905
1103	295-299	-0.8955728836941166
1103	300-301	-0.5524846525125309
2116	1	-0.03335134959792896
2116	2	0.24725730089294728
2116	3	1.2003083644950152
2116	4	0.9147344325071138
2116	5	-0.10604783234679616
2116	6	1.2233862016665569
2116	7	0.9446430306170548
2116	8	-0.8880813753509891
2116	9	-0.6520422244504367
2116	10-14	0.731777775378184
2116	15-19	0.3133921929959236
2116	20-24	-0.05865214224929005
2116	25-29	0.47369530458910347
2116	30-34	-0.7647235043287708
2116	35-39	-1.5539921448393628
2116	40-44	0.23842358922487605
2116	45-49	0.046289447983646426
2116	50-54	-0.49143902665331396
2116	55-59	0.1259688197466069
2116	60-64	0.6217400444784396
2116	65-69	-0.004276078266364891
2116	70-74	0.01177357172181459
2116	75-79	0.2434691050702611
2116	80-84	-0.35294050554590584
2116	85-89	2.914394653116311
2116	90-94	0.6526238235173132
2116	95-99	0.7492525488890927
2116	100-104	0.7135925821257771
2116	105-109	0.32896906037365525
2116	110-114	0.5237035934906231
2116	115-119	0.3207181828767709
2116	120-124	0.1982899591621461
2116	125-129	-0.14653436830208832
2116	130-134	-0.15673185105702103
2116	135-139	0.39933035758594215
2116	140-144	-0.17338637196694506
2116	145-149	0.5831347776648776
2116	150-154	0.5114163260529736
2116	155-159	0.5560256543385513
2116	160-164	-0.311420216204791
2116	165-169	0.02202238737897133
2116	170-174	0.13989843603012986
2116	175-179	-0.2266215144742496
2116	180-184	-0.21566006134434446
2116	185-189	0.1144567396034688
2116	190-194	0.41202265756648515
2116	195-199	-0.12962018339347026
2116	200-204	0.5656462492421781
2116	205-209	-0.20685415434810395
2116	210-214	0.05698550897719912
2116	215-219	-0.05323004449276958
2116	220-224	0.46375385709731276
2116	225-229	0.4145655301935012
2116	230-234	0.4087360838650582
2116	235-239	0.25278015189194036
2116	240-244	0.721113230893538
2116	245-249	0.24919184849882114
2116	250-254	0.5410057167856106
2116	255-259	0.5035426875114499
2116	260-264	0.008552176273916245
2116	265-269	0.35903726086426957
2116	270-274	1.386172526152686
2116	275-279	-0.2538577532770656
2116	280-284	-0.24870159037590867
2116	285-289	0.3833879662680548
2116	290-294	0.2371076888255086
2116	295-299	0.5391801026760987
2116	300-301	-0.42607270112146267
1117	1	0.05674276588753102
1117	2	0.18587872360742708
1117	3	0.30913300298415436
1117	4	0.40322786547089606
1117	5	0.006249284978544267
1117	6	0.9493027896727106
1117	7	1.099086905233765
1117	8	1.0130979802057283
1117	9	1.6759588733124176
1117	10-14	0.745770152660505
1117	15-19	-0.6478344950713044
1117	20-24	-1.190972296578412
1117	25-29	-0.06021641010807954
1117	30-34	-0.41067654757454264
1117	35-39	-1.048059537703729
1117	40-44	-0.34296914343360996
1117	45-49	0.2251964222917877
1117	50-54	1.3175744282964246
1117	55-59	0.89611927393873
1117	60-64	1.2212652898319405
1117	65-69	-0.6293181186769949
1117	70-74	-0.09036213318935893
1117	75-79	-0.09104899682589007
1117	80-84	-0.467506812034177
1117	85-89	2.2469634497583115
1117	90-94	0.3722140258296065
1117	95-99	0.06498140937521768
1117	100-104	0.27082888640657643
1117	105-109	0.49102677648045656
1117	110-114	0.3145200193876718
1117	115-119	-0.33694810656841057
1117	120-124	-0.2762515749689598
1117	125-129	0.09069414878879911
1117	130-134	-0.2789812107928533
1117	135-139	0.03516738624218263
1117	140-144	-0.26904409749204206
1117	145-149	0.16919680592603115
1117	150-154	0.20648583977688162
1117	155-159	0.2770135325656895
1117	160-164	0.1573334623238587
1117	165-169	0.18456366114259026
1117	170-174	-0.12682667247273116
1117	175-179	-0.49708074906055444
1117	180-184	-0.27558088336601827
1117	185-189	-0.03455097409181107
1117	190-194	0.4067253014538963
1117	195-199	-0.534347648068902
1117	200-204	0.21381410951335766
1117	205-209	0.5302960674427446
1117	210-214	0.2359937578357112
1117	215-219	0.22454894040494366
1117	220-224	0.3264178430161415
1117	225-229	0.43833118476119104
1117	230-234	-0.1670616708822248
1117	235-239	-0.18180340771034764
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2108	220-224	-0.38124330914818927
2108	225-229	-0.22932678095265047
2108	230-234	-0.07442022126824277
2108	235-239	-0.665028233384465
2108	240-244	0.3170572794044304
2108	245-249	-0.030613843142319297
2108	250-254	-0.1286397079215842
2108	255-259	-0.2022846628703583
2108	260-264	0.41886773368156227
2108	265-269	-0.12079275387416466
2108	270-274	0.5185925779504288
2108	275-279	0.418631164255995
2108	280-284	-0.35726286144005215
2108	285-289	0.14501381574654815
2108	290-294	0.03242315576032517
2108	295-299	-0.2470829757623889
2108	300-301	0.8493827613779139
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
2	544.0
3	71.0
4	52.0
5	106.0
6	124.0
7	103.0
8	78.0
9	106.0
10	565.0
11	10908.0
12	65737.0
13	220284.0
14	492470.0
15	858923.0
16	1150332.0
17	1549719.0
18	2207417.0
19	2719269.0
20	2598043.0
21	1980927.0
22	1312063.0
23	843418.0
24	574460.0
25	443508.0
26	404688.0
27	410782.0
28	428081.0
29	417858.0
30	338202.0
31	191402.0
32	61766.0
33	9047.0
34	672.0
35	155.0
36	98.0
37	17.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	5.194153197254481	13.726007139775081	65.92094783966546	15.158891823304977
2	84.05941303340855	6.031451421945892	5.443817182117474	4.46531836252809
3	5.495212168992078	79.62077618684201	3.935579412536601	10.948432231629308
4	5.0291825223759625	78.74876553026682	3.892661729271535	12.329390218085678
5	31.063675029081317	55.17988998007786	4.168032330345694	9.588402660495136
6	4.825046844309772	84.47765562190811	4.62274080007199	6.074556733710133
7	5.585521803648536	6.932165372773394	5.948442940133434	81.53386988344464
8	10.139596735287054	75.21247832659125	3.831983593600849	10.815941344520853
9	7.565538730069192	5.767298352008675	81.20975032116804	5.457412596754092
10-14	22.18309432417894	6.560192237276653	42.70619371359581	28.550519724948604
15-19	21.371552469375946	52.13677165737594	7.0643180687323035	19.427357804515815
20-24	23.365111975150967	5.249503992531863	49.35739412973776	22.02798990257941
25-29	20.765399108063644	14.553810271966919	28.766188436675883	35.91460218329356
30-34	35.57746531878097	15.361700210804335	42.88245679449374	6.178377675920952
35-39	22.82986504119671	14.635120374570429	33.62854326380666	28.906471320426203
40-44	14.933165332393056	18.42506520782201	42.284899873573195	24.356869586211737
45-49	21.425831107586202	39.247264890475094	25.05906115588524	14.26784284605346
50-54	19.790682182983975	36.63562969837788	9.900359551035681	33.67332856760247
55-59	7.055055744315128	34.328825614983536	51.404639463571655	7.211479177129679
60-64	5.66286407492702	24.45014477827292	20.209721921417987	49.67726922538207
65-69	16.638864334075446	17.347885241782738	20.578399379575472	45.434851044566344
70-74	21.209711746970772	28.266513412430783	25.566893145457968	24.956881695140474
75-79	13.135829477513825	43.9428036538015	20.373310764560483	22.548056104124196
80-84	31.763842494536387	29.427306808718573	28.211713028488	10.597137668257039
85-89	6.038429043384283	13.99878820569002	5.453601072672585	74.50918167825311
90-94	26.557781946591184	27.738664438046595	23.639231032767277	22.06432258259494
95-99	7.086892815769246	14.441562158262444	36.405694267845966	42.065850758122345
100-104	5.62353307894252	38.541407748439966	38.347307915173666	17.487751257443847
105-109	6.015253908637971	22.904168089651037	44.364372800136834	26.716205201574155
110-114	6.858031321630115	27.2296772838791	30.371438711927745	35.54085268256304
115-119	20.784794079486325	22.088503459680357	23.33395147990707	33.79275098092624
120-124	11.436530894632682	46.8115542320796	18.599318990282914	23.152595883004807
125-129	4.892888468908519	44.21113595556063	5.417212831397694	45.478762744133164
130-134	6.694822251888819	50.6964999376736	5.697452692254071	36.9112251181835
135-139	16.78075418548651	37.71322864889291	14.911668667259878	30.594348498360706
140-144	13.742773056531025	29.80111459038288	15.207701161430641	41.24841119165546
145-149	8.449115894054943	22.583936336778653	33.35930648976721	35.607641279399196
150-154	6.002865322828843	24.475785212448027	43.71841912601174	25.802930338711388
155-159	6.71945522475135	25.78448838073121	48.65275844817411	18.843297946343327
160-164	8.159374853328453	24.503917498179963	30.941515751592252	36.39519189689933
165-169	11.470028864399302	39.80863342957288	19.405779695585494	29.315558010442327
170-174	8.643267001642142	37.6354367470273	26.038345110724258	27.6829511406063
175-179	13.770304060170277	43.85095992858255	12.610226676751502	29.768509334495675
180-184	10.279181386046917	44.35855089428542	11.018127693588008	34.34414002607965
185-189	13.349077013806957	24.875633764595968	24.32794893185118	37.4473402897459
190-194	10.626775416310146	22.08307426860805	41.39275166187624	25.897398653205567
195-199	9.68011778364135	34.209186063610005	23.388851309764696	32.72184484298395
200-204	7.075172048703017	25.37640040666717	42.80079570905175	24.74763183557807
205-209	6.825130665911113	22.910937666679587	51.88582242192707	18.378109245482232
210-214	11.841014715492587	35.433475261238954	27.5355837282041	25.189926295064364
215-219	9.88760823686161	39.226075828775606	26.507108038967463	24.37920789539532
220-224	12.993755203875812	31.335735519337526	25.47884159096177	30.191667685824893
225-229	15.646300793098836	32.15700349629023	17.525191193959845	34.67150451665109
230-234	14.78727546703933	28.328559414898475	19.838719458436522	37.04544565962567
235-239	13.069402992247154	31.897523487813977	18.262963091565222	36.77011042837365
240-244	11.101099094260201	31.137985262349442	24.579932211373436	33.180983432016916
245-249	13.62235018433381	32.791646844921246	24.293473469543954	29.292529501200992
250-254	11.775224155596469	30.152576181301505	26.900674650264516	31.17152501283751
255-259	15.310812791951728	33.97918528166194	21.662668395506813	29.047333530879516
260-264	13.362283261230154	34.52489890376295	19.1252882095005	32.9875296255064
265-269	12.28139736754017	34.95849106144923	19.9773075380681	32.7828040329425
270-274	11.739593048787093	35.75282188787477	17.69974681087136	34.80783825246678
275-279	11.53121723413571	34.60019696972682	16.855380073917043	37.013205722220434
280-284	12.07878076708866	34.083133132289376	17.818596900426048	36.01948920019592
285-289	12.80823621791145	35.230587383039904	14.591952449606193	37.36922394944246
290-294	13.434366182591685	36.29974290079801	14.665616810450954	35.600274106159354
295-299	14.941007645490098	35.48804585141633	14.338282553032695	35.23266395006088
300-301	9.641231574093219	40.49902329238297	9.192663797365292	40.66708133615852
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	228.0
1	47.75
2	18.5
3	19.75
4	18.25
5	18.5
6	18.25
7	18.25
8	17.75
9	17.5
10	16.5
11	18.25
12	20.5
13	24.0
14	28.5
15	25.25
16	29.0
17	33.25
18	37.25
19	49.25
20	59.0
21	74.0
22	108.75
23	201.5
24	349.5
25	701.5
26	1368.25
27	2686.0
28	5149.75
29	9872.25
30	18159.5
31	31843.0
32	53053.5
33	83940.5
34	126360.5
35	181056.0
36	243209.5
37	302772.0
38	345845.75
39	367185.5
40	374151.0
41	378553.0
42	375893.75
43	373215.5
44	369872.25
45	362513.75
46	334593.25
47	302983.0
48	279640.25
49	257991.25
50	231883.0
51	205324.5
52	176881.75
53	143950.75
54	109491.0
55	80660.5
56	58835.5
57	43542.5
58	32558.5
59	24680.75
60	19247.0
61	15491.25
62	13004.5
63	11278.25
64	9988.0
65	8979.25
66	8196.5
67	7603.75
68	7035.0
69	6452.75
70	5872.25
71	5255.5
72	4531.5
73	3702.5
74	2841.25
75	2075.25
76	1513.75
77	1068.25
78	775.0
79	575.75
80	445.5
81	347.5
82	276.75
83	226.5
84	173.25
85	144.75
86	117.5
87	109.5
88	94.0
89	91.0
90	84.0
91	80.5
92	77.0
93	70.25
94	72.0
95	73.25
96	76.5
97	84.0
98	92.25
99	140.25
100	321.66666666666583
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0032707866656610683
2	0.002088949328464993
3	0.0014202782034724766
4	0.0016120675959121907
5	0.0020630318430001665
6	0.0022340872470680197
7	0.0024673446162514555
8	0.002581381552296691
9	0.0035869799883319485
10-14	0.0026041889395057383
15-19	0.0028094554243871615
20-24	0.0033817135034505245
25-29	0.0034387319714731424
30-34	0.0033485391220555473
35-39	0.004246320818557127
40-44	0.0034957504394957594
45-49	0.0037321179069349747
50-54	0.0062969122685341775
55-59	0.0053047909249406295
60-64	0.005789966252842177
65-69	0.003566245999960087
70-74	0.0033806768040319313
75-79	0.0035185578267048065
80-84	0.005667635721448196
85-89	0.006543646730159323
90-94	0.005878085703422585
95-99	0.005486213323194413
100-104	0.005154469509244638
105-109	0.005005184792967238
110-114	0.006806968382481957
115-119	0.00514824931273308
120-124	0.005754718472610013
125-129	0.008136017037118245
130-134	0.008359944111534344
135-139	0.008897991109784136
140-144	0.009078376808619327
145-149	0.0067862343941100965
150-154	0.006862950151085982
155-159	0.009223514727222354
160-164	0.007482896403404624
165-169	0.006796601388296026
170-174	0.008230356684210212
175-179	0.006465894273764843
180-184	0.008056191181886581
185-189	0.008452210359789126
190-194	0.007510887287706636
195-199	0.007455942218521206
200-204	0.007915200060957925
205-209	0.008374457903394646
210-214	0.008245907175489108
215-219	0.536251434856789
220-224	0.008114246349327791
225-229	0.008619118966182605
230-234	0.00949409327547514
235-239	0.0102156360708159
240-244	0.00839933868944088
245-249	0.011161105940572761
250-254	0.008524779319090637
255-259	0.010320342712093799
260-264	0.009644414691171131
265-269	0.5342516416783231
270-274	0.5364577380410891
275-279	0.008920798496993183
280-284	0.009775038817913854
285-289	0.01024259025569932
290-294	0.011623473881265261
295-299	0.01108957368068984
300-301	0.010022291629248297
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
301	1.9291995E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.17499619015606
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.78641907442699	55.83422414301867
2	4.088243918507936	4.920201244412875
3	1.0756674398019925	1.9418485227587947
4	0.6276261062890335	1.510695942191403
5	0.30748386826044577	0.9251420300553388
6	0.21073490027783598	0.7608583092811018
7	0.16358328171498404	0.6890536347780674
8	0.10043293934053389	0.48348413937462414
9	0.06079696657852771	0.32926115090123553
>10	0.4987476876862857	5.555001669177278
>50	0.04265568297910549	1.786931358844824
>100	0.03130078734992266	3.8010161755909078
>500	0.0029964768648101684	1.2410201981428468
>1k	0.002333530212737124	2.7520989137999536
>5k	4.094114010038634E-4	1.6927275436369948
>10k+	5.679283076864023E-4	15.776435024035035
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGAAGACATTCTTGGCAAATGCTTTCGCTTTAGGGCGTCCTTCTGCGATC	697750	3.616785096616498	No Hit
TGAAAACATTCTTGGCAAATGCTTTCGCTGTTGGACGTCCCGCTGCGGTC	227253	1.1779652648676304	No Hit
TGAAAACATCCTTGGTAAATGCTTTCGCAGTCGTTCGTCGTGGAAAAATC	136546	0.707785794056032	No Hit
TGAAAACATCTTTGGCAAATGCTTTCGCATTAGTTAGTCCTTAATAAATC	120992	0.6271616802720507	No Hit
TGAAAACATCCTTGGCAAATGCTTTCGCAGTAGTTCGTCTTTCATAAATC	99158	0.5139852047442476	No Hit
CGAAGACATTCTTGGCAAATGCTTTCGCTTTAGGTCGTCCTTCTGCGATC	86406	0.44788524981475475	No Hit
TGAAAACATTCTTGGCAAATGCTTTCGCTGTTGGTCGTCCCGCTGCGGTC	82942	0.42992961588472317	No Hit
TGAAAACATCCTTGATAAATGCTTTCGCAGTCGTTCGTCGTGGAAAAATC	78825	0.40858915835298526	No Hit
TGAAAACATCCTTGGCAAATGCTTTCGCAGTAGTTCGTCTCCAACAAATC	64555	0.33462065483637127	No Hit
TGAAAACGTCCTTGGCAAATGCTTTCGCAGTAGTTAGTCTTCAATAAATC	64406	0.33384831376951946	No Hit
TGAAAACGTCCTTGGCAAATGCTTTCGCAGTAGTTAGTCTTCAGTAAATC	60923	0.3157941933947215	No Hit
TGAAAACATCCTTGGCAAATGCTTTCGCATAAGTTAGTCTTTAACAAATC	56062	0.29059721402581745	No Hit
TGAAAACATCCTTGGCAAATGCTTTCGCAGTTGTTCGTCTTTCATAAATC	54140	0.28063453261313825	No Hit
TGAAAACGTCCTTGGCAAATGCTTTCGCAGTTGTTAGTCTTCCGTAAATC	48399	0.2508760758024248	No Hit
TGAAAACATTCTTGGCAAATGCTTTCGCTGTTGGACGTCCCGCTGCGGTA	48140	0.2495335500553468	No Hit
TGAAAACATCCTTGGTAAATGCTTTCGCAGTCGTTCGTCGTGGGAAAATC	47116	0.24422564903215038	No Hit
TGAAAACATCCTTGGCAAATGCTTTCGCAGTAGTTAGTCTTTAGAAAATC	44529	0.23081594205264933	No Hit
TGAAAACATGCTTGGCAAATGCTTTCGCATTAGTTAGTCTTTAACAAATC	44511	0.23072263910497592	No Hit
TGAAAACATCCTTGGCAAATGCTTTCGCATTTGTTAGTCTTTGACAAATC	37693	0.1953815559251389	No Hit
TGAAAACATCCTTGGCAAATGCTTTCGCATTAGTTAGTCCTTAATAAATC	37238	0.1930230647478397	No Hit
TGAAAACGTCCTTGGCAAATGCTTTCGCAGATGTTAGTCTTCCGTAAATC	36040	0.18681323523046736	No Hit
CAGGCACGCACTTGAAGAACGCCTTCGCTGATGTTCATCTTAATAAAATC	35655	0.18481758884967572	No Hit
TGAAAACATCCTTGGCAAATGCTTTCGCAGTAGTTAGTCTTCAGTAAATC	35295	0.18295152989620825	No Hit
TGAAAACATCCTTGGCAAATGCTTTCGCAGAAGTTAGTCTTTCATAAATC	34243	0.1774984909544088	No Hit
TGAAAACATTCTTGGCGAACGCCTTCGCTGCTGTTCGTCCTGTGGCGATC	31401	0.1627669922162016	No Hit
TGAAGACATTCTTGGCAAATGCTTTCGCTTTTGGGCGTCCCCCTGCGGTC	31357	0.1625389183441111	No Hit
TGAAAACATCCTTGGCAAATGCTTTCGCATTAGTTAGTCTTTAATAAATC	30322	0.1571739988528921	No Hit
TGAAAACATCCTTGGTAAATGCTTTCGCATTTGTTAGTCTTTGTCAAATC	29536	0.15309977013782142	No Hit
TGAAAACATTCTTGGCAAATGCTTTCGCTGTTGGTCGTCTCGCTGCGGTC	29376	0.15227041060294697	No Hit
TGAAGACATCCTTGGCAAATGCTTTCGCATTAGTTAGTCTTCAGGAAATC	29373	0.15225486011166808	No Hit
TGAAAACATCCTTGGCAAATGCTTTCGCAGTAGTTAGTCTTCAATAAATC	28686	0.14869379760880094	No Hit
CGAAGACATTCTTGGCAAATGCTTTCGCTTTAGGGCGTCTTTCTGCGATC	28118	0.14574957125999669	No Hit
CGAAGACATTCTTGGCAAATGCTTTCGCTTTAGTGCGTCCTTCTGCGATC	26119	0.1353877605711592	No Hit
TGAAAACATCCTTGGTAAATGCTTTCGCAGTAGTTCGTCTTCCATAAATC	25982	0.13467762146942294	No Hit
TGAAAACATCTTTGGCAAATGCTTTCGCATTAGTTAGTCCTTAATAAATA	25552	0.13244871771944788	No Hit
CGAAGACATTCTTGGCAAATGCTTTCGCTTTAGGGCGTCCTTCTGAGATC	25489	0.1321221574025911	No Hit
CGAAAACATCCTTGGCAAATGCTTTCGCAGTGGTTCGTCTTTCGTAAATC	25304	0.1311632104403925	No Hit
TGAAAACATCCTTGGCAAATGCTTTCGCAGAAGTTCGTCTTTCATAAATC	25104	0.13012651102179945	No Hit
TGAAAACATTCTTGGCAAATGCTTTCGCTGTTGGACGTCTCGCTGCGGTC	24658	0.12781467131833696	No Hit
TGAAGACATTCTTGGCAAATGCTTTCGCTTTAGGGCGTCCTTCTGCGATC	23596	0.12230979740560786	No Hit
TGAAAACATCCTGGACAGATGCCTTCGCAGTCGTTCGTCGTGGAAAAATC	21260	0.11020114819644106	No Hit
TGAAAACATCCTTGGTAAATGCTTTCGCAGTCGTTCGTCGTGAAAAAATC	20653	0.10705476546101116	No Hit
TGAAAACATTCTTGGCAAATGCTTTCGCTTTAGTTAGTCTTGCGACGGTC	20294	0.10519389000463664	No Hit
TGAAAACATCCTTGGCAAATGCTTTCGCATAAGTTAGTCTTTAATAAATC	20176	0.10458223734766674	No Hit
CGCAGACATTCTTGGCAAATGCTTTCGCTTTAGGGCGTCCTTCTGCGATC	19540	0.10128553319654085	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0012129383197538668	0.0	0.0
2	0.001218121816846832	0.0	0.0
3	0.0012284888110327625	1.0366994185930486E-5	0.0
4	0.001238855805218693	1.0366994185930486E-5	0.0
5	0.0012906907761483454	1.0366994185930486E-5	0.0
6	0.0013062412674272412	1.0366994185930486E-5	0.0
7	0.0013217917587061369	1.0366994185930486E-5	0.0
8	0.0013321587528920674	1.0366994185930486E-5	0.0
9	0.0013321587528920674	1.0366994185930486E-5	0.0
10-14	0.0014244250011468487	1.1403693604523535E-5	3.110098255779146E-6
15-19	0.0015851134110287713	1.555049127889573E-5	5.183497092965243E-6
20-24	0.0017997101906775323	1.555049127889573E-5	5.183497092965243E-6
25-29	0.001986316086024281	1.555049127889573E-5	5.183497092965243E-6
30-34	0.0022848855185790785	3.213768197638451E-5	5.183497092965243E-6
35-39	0.002672611101132879	3.62844796507567E-5	3.110098255779146E-5
40-44	0.003157786429034426	3.62844796507567E-5	3.110098255779146E-5
45-49	0.003755961993562615	3.62844796507567E-5	3.110098255779146E-5
50-54	0.004452624002857143	3.62844796507567E-5	3.110098255779146E-5
55-59	0.005360772693544655	3.62844796507567E-5	3.110098255779146E-5
60-64	0.006671160758646268	3.62844796507567E-5	3.4211080813570606E-5
65-69	0.00825627416967504	3.62844796507567E-5	2.5917485464826214E-4
70-74	0.009983415401051058	4.457807499950109E-5	2.5917485464826214E-4
75-79	0.01237197086148944	4.6651473836687183E-5	2.5917485464826214E-4
80-84	0.015157582199248964	4.6651473836687183E-5	2.5917485464826214E-4
85-89	0.018671993228279397	4.6651473836687183E-5	2.5917485464826214E-4
90-94	0.023065525364276737	4.6651473836687183E-5	3.607713976703809E-4
95-99	0.028362022693868623	4.8724872673873285E-5	4.665147383668718E-4
100-104	0.03503940364902645	5.1834970929652425E-5	4.716982354598371E-4
105-109	0.04348643051172261	5.1834970929652425E-5	4.789551313899884E-4
110-114	0.054138517037766176	5.1834970929652425E-5	4.89322125575919E-4
115-119	0.0664980475062325	5.1834970929652425E-5	4.976157209246634E-4
120-124	0.08013271825956829	5.1834970929652425E-5	4.976157209246634E-4
125-129	0.0953390253314911	5.1834970929652425E-5	4.976157209246634E-4
130-134	0.11190651874002663	5.1834970929652425E-5	4.976157209246634E-4
135-139	0.12907218771309031	5.1834970929652425E-5	4.976157209246634E-4
140-144	0.14725174871753804	5.1834970929652425E-5	5.266433046452687E-4
145-149	0.16616114611267524	5.1834970929652425E-5	5.442671947613505E-4
150-154	0.18562103089908538	5.1834970929652425E-5	5.55670888365874E-4
155-159	0.2057133023308372	5.1834970929652425E-5	5.774415761563281E-4
160-164	0.2271149251282721	5.1834970929652425E-5	5.815883738307002E-4
165-169	0.2486679060408216	5.1834970929652425E-5	5.857351715050724E-4
170-174	0.2698829229429097	5.1834970929652425E-5	5.857351715050724E-4
175-179	0.29105128837116123	5.1834970929652425E-5	5.857351715050724E-4
180-184	0.3125120030354559	5.1834970929652425E-5	5.888452697608516E-4
185-189	0.33475853585904414	5.1834970929652425E-5	6.272031482487943E-4
190-194	0.35710562852623584	5.1834970929652425E-5	6.593408302251789E-4
195-199	0.3788877200102944	5.1834970929652425E-5	6.925152116201564E-4
200-204	0.4015074646245762	5.1834970929652425E-5	7.2776299185232E-4
205-209	0.42358708884177093	5.1834970929652425E-5	7.49533679642774E-4
210-214	0.4446528210275817	5.1834970929652425E-5	7.567905755729254E-4
215-219	0.46544175446862807	5.1834970929652425E-5	7.816713616191587E-4
220-224	0.48677495510443586	5.3908369766838526E-5	7.920383558050892E-4
225-229	0.5078655680762928	5.701846802261768E-5	0.0013103880651016133
230-234	0.5287312172743149	5.701846802261768E-5	0.0013342321517292536
235-239	0.5479619914892162	5.701846802261768E-5	0.0014109479087051392
240-244	0.5641821905925231	5.701846802261768E-5	0.0014959572610297692
245-249	0.5803018298522262	5.701846802261768E-5	0.0016535355726559123
250-254	0.5967324789375075	5.701846802261768E-5	0.001680489757539332
255-259	0.6127235674693052	6.116526569698987E-5	0.0017136641389343093
260-264	0.6276966171720447	9.433964709196742E-5	0.0017385449249805423
265-269	0.6418475642358398	9.848644476633962E-5	0.0017686092081197406
270-274	0.6553516108624329	1.0366994185930485E-4	0.0017986734912589393
275-279	0.6671627273384635	1.0366994185930485E-4	0.001838068069165475
280-284	0.6781610714703171	1.0366994185930485E-4	0.001854655259862964
285-289	0.6883414597609009	1.0366994185930485E-4	0.0018619121557931152
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCTTGG	650010	0.0	293.2073	9
ATTCTTG	473665	0.0	288.78302	8
ATCCTTG	595855	0.0	288.06836	8
TGAAAAC	670420	0.0	286.8128	1
TTCTTGG	510695	0.0	284.08734	9
AAGACAT	356515	0.0	274.93182	3
AGACATT	251620	0.0	266.57513	4
TGAAGAC	156580	0.0	265.6678	1
GACATTC	259070	0.0	265.2907	5
AAAACAT	660610	0.0	263.14697	3
GAAAACA	690155	0.0	262.7515	2
CGAAGAC	166215	0.0	259.9483	1
ATCTTTG	62015	0.0	258.72736	8
GAAGACA	395685	0.0	258.5301	2
CATCCTT	661510	0.0	256.44424	7
AAACATC	495025	0.0	253.01584	4
ACATCCT	664440	0.0	252.48181	6
AACATCC	489255	0.0	250.07513	5
ACATTCT	550080	0.0	246.02762	6
GACATCC	153090	0.0	244.6758	5
>>END_MODULE