FastQCFastQC Report
Tue 25 Aug 2015
000000000-AHV8M l01n03.33300000033c8a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AHV8M l01n03.33300000033c8a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19291995
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAAGACATTCTTGGCAAATGCTTTCGCTTTAGGGCGTCCTTCTGCGATC6977503.616785096616498No Hit
TGAAAACATTCTTGGCAAATGCTTTCGCTGTTGGACGTCCCGCTGCGGTC2272531.1779652648676304No Hit
TGAAAACATCCTTGGTAAATGCTTTCGCAGTCGTTCGTCGTGGAAAAATC1365460.707785794056032No Hit
TGAAAACATCTTTGGCAAATGCTTTCGCATTAGTTAGTCCTTAATAAATC1209920.6271616802720507No Hit
TGAAAACATCCTTGGCAAATGCTTTCGCAGTAGTTCGTCTTTCATAAATC991580.5139852047442476No Hit
CGAAGACATTCTTGGCAAATGCTTTCGCTTTAGGTCGTCCTTCTGCGATC864060.44788524981475475No Hit
TGAAAACATTCTTGGCAAATGCTTTCGCTGTTGGTCGTCCCGCTGCGGTC829420.42992961588472317No Hit
TGAAAACATCCTTGATAAATGCTTTCGCAGTCGTTCGTCGTGGAAAAATC788250.40858915835298526No Hit
TGAAAACATCCTTGGCAAATGCTTTCGCAGTAGTTCGTCTCCAACAAATC645550.33462065483637127No Hit
TGAAAACGTCCTTGGCAAATGCTTTCGCAGTAGTTAGTCTTCAATAAATC644060.33384831376951946No Hit
TGAAAACGTCCTTGGCAAATGCTTTCGCAGTAGTTAGTCTTCAGTAAATC609230.3157941933947215No Hit
TGAAAACATCCTTGGCAAATGCTTTCGCATAAGTTAGTCTTTAACAAATC560620.29059721402581745No Hit
TGAAAACATCCTTGGCAAATGCTTTCGCAGTTGTTCGTCTTTCATAAATC541400.28063453261313825No Hit
TGAAAACGTCCTTGGCAAATGCTTTCGCAGTTGTTAGTCTTCCGTAAATC483990.2508760758024248No Hit
TGAAAACATTCTTGGCAAATGCTTTCGCTGTTGGACGTCCCGCTGCGGTA481400.2495335500553468No Hit
TGAAAACATCCTTGGTAAATGCTTTCGCAGTCGTTCGTCGTGGGAAAATC471160.24422564903215038No Hit
TGAAAACATCCTTGGCAAATGCTTTCGCAGTAGTTAGTCTTTAGAAAATC445290.23081594205264933No Hit
TGAAAACATGCTTGGCAAATGCTTTCGCATTAGTTAGTCTTTAACAAATC445110.23072263910497592No Hit
TGAAAACATCCTTGGCAAATGCTTTCGCATTTGTTAGTCTTTGACAAATC376930.1953815559251389No Hit
TGAAAACATCCTTGGCAAATGCTTTCGCATTAGTTAGTCCTTAATAAATC372380.1930230647478397No Hit
TGAAAACGTCCTTGGCAAATGCTTTCGCAGATGTTAGTCTTCCGTAAATC360400.18681323523046736No Hit
CAGGCACGCACTTGAAGAACGCCTTCGCTGATGTTCATCTTAATAAAATC356550.18481758884967572No Hit
TGAAAACATCCTTGGCAAATGCTTTCGCAGTAGTTAGTCTTCAGTAAATC352950.18295152989620825No Hit
TGAAAACATCCTTGGCAAATGCTTTCGCAGAAGTTAGTCTTTCATAAATC342430.1774984909544088No Hit
TGAAAACATTCTTGGCGAACGCCTTCGCTGCTGTTCGTCCTGTGGCGATC314010.1627669922162016No Hit
TGAAGACATTCTTGGCAAATGCTTTCGCTTTTGGGCGTCCCCCTGCGGTC313570.1625389183441111No Hit
TGAAAACATCCTTGGCAAATGCTTTCGCATTAGTTAGTCTTTAATAAATC303220.1571739988528921No Hit
TGAAAACATCCTTGGTAAATGCTTTCGCATTTGTTAGTCTTTGTCAAATC295360.15309977013782142No Hit
TGAAAACATTCTTGGCAAATGCTTTCGCTGTTGGTCGTCTCGCTGCGGTC293760.15227041060294697No Hit
TGAAGACATCCTTGGCAAATGCTTTCGCATTAGTTAGTCTTCAGGAAATC293730.15225486011166808No Hit
TGAAAACATCCTTGGCAAATGCTTTCGCAGTAGTTAGTCTTCAATAAATC286860.14869379760880094No Hit
CGAAGACATTCTTGGCAAATGCTTTCGCTTTAGGGCGTCTTTCTGCGATC281180.14574957125999669No Hit
CGAAGACATTCTTGGCAAATGCTTTCGCTTTAGTGCGTCCTTCTGCGATC261190.1353877605711592No Hit
TGAAAACATCCTTGGTAAATGCTTTCGCAGTAGTTCGTCTTCCATAAATC259820.13467762146942294No Hit
TGAAAACATCTTTGGCAAATGCTTTCGCATTAGTTAGTCCTTAATAAATA255520.13244871771944788No Hit
CGAAGACATTCTTGGCAAATGCTTTCGCTTTAGGGCGTCCTTCTGAGATC254890.1321221574025911No Hit
CGAAAACATCCTTGGCAAATGCTTTCGCAGTGGTTCGTCTTTCGTAAATC253040.1311632104403925No Hit
TGAAAACATCCTTGGCAAATGCTTTCGCAGAAGTTCGTCTTTCATAAATC251040.13012651102179945No Hit
TGAAAACATTCTTGGCAAATGCTTTCGCTGTTGGACGTCTCGCTGCGGTC246580.12781467131833696No Hit
TGAAGACATTCTTGGCAAATGCTTTCGCTTTAGGGCGTCCTTCTGCGATC235960.12230979740560786No Hit
TGAAAACATCCTGGACAGATGCCTTCGCAGTCGTTCGTCGTGGAAAAATC212600.11020114819644106No Hit
TGAAAACATCCTTGGTAAATGCTTTCGCAGTCGTTCGTCGTGAAAAAATC206530.10705476546101116No Hit
TGAAAACATTCTTGGCAAATGCTTTCGCTTTAGTTAGTCTTGCGACGGTC202940.10519389000463664No Hit
TGAAAACATCCTTGGCAAATGCTTTCGCATAAGTTAGTCTTTAATAAATC201760.10458223734766674No Hit
CGCAGACATTCTTGGCAAATGCTTTCGCTTTAGGGCGTCCTTCTGCGATC195400.10128553319654085No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTTGG6500100.0293.20739
ATTCTTG4736650.0288.783028
ATCCTTG5958550.0288.068368
TGAAAAC6704200.0286.81281
TTCTTGG5106950.0284.087349
AAGACAT3565150.0274.931823
AGACATT2516200.0266.575134
TGAAGAC1565800.0265.66781
GACATTC2590700.0265.29075
AAAACAT6606100.0263.146973
GAAAACA6901550.0262.75152
CGAAGAC1662150.0259.94831
ATCTTTG620150.0258.727368
GAAGACA3956850.0258.53012
CATCCTT6615100.0256.444247
AAACATC4950250.0253.015844
ACATCCT6644400.0252.481816
AACATCC4892550.0250.075135
ACATTCT5500800.0246.027626
GACATCC1530900.0244.67585
Produced by FastQC (version 0.11.2)