FastQCFastQC Report
Tue 25 Aug 2015
000000000-AHV8M l01n02.33200000033c8d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AHV8M l01n02.33200000033c8d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19291995
Sequences flagged as poor quality0
Sequence length12
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTGTACCCAA9426064.885995460811595No Hit
TACAGATGGCTC8725334.522772269016243No Hit
CTGCTAACGCAA8365694.33635297956484No Hit
CGGAGCTATGGT8330204.317956748381906No Hit
GCGATATATCGC8324554.315028072524382No Hit
GACTTTCCCTCG8298254.301395475169882No Hit
TCCAAAGTGTTC8002684.148186851593109No Hit
ATCCCGAATTTG7970554.131532275433412No Hit
TTAGGGCTCGTA7772814.029033803917117No Hit
TCACGGGAGTTG7511793.8937341627965383No Hit
ATGTGCACGACT7289623.7785724078821294No Hit
TCTAGCGTAGTG7260063.7632499904753245No Hit
TAGCTCGTAACT7055603.657268208912557No Hit
AAGAGATGTCGA6968643.6121925181921313No Hit
ACGCGCAGATAC6762133.505148119725306No Hit
GTTGGTCAATCT6758133.5030747208881197No Hit
GGATGGTGTTGC6567823.404427587711898No Hit
CAGTGCATATGC6399253.3170493772157834No Hit
TCGAGGACTGCA6170993.1987308725717583No Hit
TAGGATTGCTCG4115292.1331593751708935No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGGG650.00126185656.00006531
TTTGGGC650.00126185656.00006531
CGGTGCA1303.6470738E-96.00006531
TCAAGGC1303.6470738E-96.00006531
TTTAGCG1900.06.0000651
TTAGGGG550.0091599476.0000651
TCTTGCG3550.06.0000651
ACACGGG1653.6379788E-126.0000651
TCGGGGA600.0033962716.0000651
GCACGGG2300.06.0000651
TTCCGGC550.0091599476.0000651
TCGATGA2150.06.0000651
CCTCGCC953.4294917E-66.0000651
ACGTGTG600.0033962716.0000651
TTCGGTC600.0033962716.0000651
TTCGGGC3250.06.0000651
CCGCGCT1552.7284841E-116.0000651
CCGCGCC751.7511961E-46.0000651
GTTGGGC1156.826485E-86.0000651
ATTTGCG550.0091599476.0000651
Produced by FastQC (version 0.11.2)