##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AHV8M l01n01.33100000033c80.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	19291995
Sequences flagged as poor quality	0
Sequence length	301
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.234235184075054	34.0	34.0	34.0	32.0	34.0
2	33.636279036978806	34.0	34.0	34.0	34.0	34.0
3	33.76489600997719	34.0	34.0	34.0	34.0	34.0
4	33.662574347546744	34.0	34.0	34.0	34.0	34.0
5	33.78411133737076	34.0	34.0	34.0	34.0	34.0
6	37.33863957563746	38.0	38.0	38.0	37.0	38.0
7	37.3442827970876	38.0	38.0	38.0	37.0	38.0
8	37.16255353580591	38.0	38.0	38.0	36.0	38.0
9	37.07851458597206	38.0	38.0	38.0	36.0	38.0
10-14	37.09793855949061	38.0	37.8	38.0	36.2	38.0
15-19	37.16162697533355	38.0	38.0	38.0	36.4	38.0
20-24	36.89219586673126	38.0	37.0	38.0	34.8	38.0
25-29	37.22227548783835	38.0	38.0	38.0	36.8	38.0
30-34	37.21632420078898	38.0	38.0	38.0	36.8	38.0
35-39	37.22821903074306	38.0	38.0	38.0	36.8	38.0
40-44	37.23440202011248	38.0	38.0	38.0	36.6	38.0
45-49	36.925537934257186	38.0	37.6	38.0	35.2	38.0
50-54	36.19216510267601	38.0	37.2	38.0	32.6	38.0
55-59	36.02191956819396	38.0	37.0	38.0	32.4	38.0
60-64	35.027584601799866	38.0	36.2	38.0	26.4	38.0
65-69	34.6339474896194	38.0	35.8	38.0	24.4	38.0
70-74	34.98172133053113	38.0	36.2	38.0	26.2	38.0
75-79	34.89865014997153	38.0	36.2	38.0	25.8	38.0
80-84	34.46216071484572	38.0	35.6	38.0	23.4	38.0
85-89	34.439888357839614	38.0	35.6	38.0	23.4	38.0
90-94	33.99240623896077	38.0	34.6	38.0	22.0	38.0
95-99	34.07361413892135	38.0	34.4	38.0	22.8	38.0
100-104	33.981371029797586	38.0	34.0	38.0	22.2	38.0
105-109	34.00604921367645	38.0	34.2	38.0	22.4	38.0
110-114	33.80066600680749	38.0	34.0	38.0	21.4	38.0
115-119	33.674391217704546	38.0	34.0	38.0	20.8	38.0
120-124	33.33968345938302	37.8	33.2	38.0	18.8	38.0
125-129	33.33341228836106	37.4	33.4	38.0	19.6	38.0
130-134	33.044963830853156	37.0	32.8	38.0	18.4	38.0
135-139	33.08044553194214	37.2	33.0	38.0	17.6	38.0
140-144	33.05758232883639	37.0	32.6	38.0	17.8	38.0
145-149	32.80588729159426	37.0	32.0	38.0	16.2	38.0
150-154	32.73236727461312	37.0	31.6	38.0	17.4	38.0
155-159	32.44597991032032	37.0	30.6	38.0	14.2	38.0
160-164	32.19752895436683	37.0	30.0	38.0	11.8	38.0
165-169	32.167762162492785	37.0	30.0	38.0	11.6	38.0
170-174	32.11351758073751	37.0	29.8	38.0	12.2	38.0
175-179	31.75214780016271	37.0	28.4	38.0	11.0	38.0
180-184	32.11535235210251	37.0	29.6	38.0	11.6	38.0
185-189	31.774706234373376	37.0	28.2	38.0	11.0	38.0
190-194	31.47595610510992	36.8	27.4	38.0	11.0	38.0
195-199	31.034379057220367	36.6	26.4	38.0	11.0	38.0
200-204	30.672433307182594	36.0	25.6	38.0	11.0	38.0
205-209	29.93369117087165	35.0	23.8	38.0	11.0	38.0
210-214	30.248393864916512	35.0	24.0	38.0	11.0	38.0
215-219	29.611749204786754	34.2	23.6	38.0	11.0	38.0
220-224	29.43146817112487	34.0	22.8	37.8	11.0	38.0
225-229	28.724602686243696	34.0	21.8	37.0	10.8	38.0
230-234	28.57058025362333	34.0	21.2	37.0	10.8	38.0
235-239	28.527505320211834	34.0	21.2	37.0	10.4	38.0
240-244	28.20709576173952	34.0	20.6	37.0	10.4	38.0
245-249	28.123423181480195	33.4	20.0	37.0	10.0	38.0
250-254	26.431400142909013	29.2	17.8	36.4	9.2	37.6
255-259	25.358787030579265	26.8	16.2	36.0	9.4	37.4
260-264	24.166278438284895	25.0	14.8	34.2	8.8	37.2
265-269	23.679453327662586	24.2	14.2	33.4	8.6	37.0
270-274	22.12058537232671	22.0	13.0	31.4	8.4	36.2
275-279	21.590068481771844	21.4	11.4	31.2	8.4	36.2
280-284	21.211046840930656	20.8	10.6	30.2	8.4	36.0
285-289	22.322683755619884	22.4	10.0	32.6	8.6	36.2
290-294	20.687663033294378	20.4	9.6	29.4	8.0	35.4
295-299	19.487010824956155	18.8	8.8	27.4	7.8	34.2
300-301	15.658025984352577	14.5	8.0	21.5	7.0	28.5
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	-6.972404644136255
1101	2	-1.3013518716206676
1101	3	-0.12502664388973272
1101	4	0.14154107863055287
1101	5	0.041062663271624444
1101	6	0.029601194530663122
1101	7	0.022186365462850688
1101	8	0.056969841800970755
1101	9	0.17567656340484916
1101	10-14	0.20381994953989846
1101	15-19	0.2766307576520859
1101	20-24	0.3195253773254123
1101	25-29	0.3199786290226996
1101	30-34	0.2949735670685243
1101	35-39	0.22668734941889568
1101	40-44	0.3213389893324745
1101	45-49	0.3725643974205184
1101	50-54	0.5460931582176016
1101	55-59	0.524951797562693
1101	60-64	0.5966430379107095
1101	65-69	0.7310526778991502
1101	70-74	0.523574644905807
1101	75-79	0.45685005310728144
1101	80-84	0.5889423990040541
1101	85-89	0.6233877299527961
1101	90-94	0.8454345989683105
1101	95-99	0.6668829243880268
1101	100-104	0.688497055004504
1101	105-109	0.712960298303507
1101	110-114	0.7671991680881831
1101	115-119	0.7718374288330949
1101	120-124	1.0111650473622618
1101	125-129	0.9167057131561904
1101	130-134	0.9132756543264051
1101	135-139	0.9217981538905491
1101	140-144	0.8429794284497873
1101	145-149	0.9970858836413825
1101	150-154	0.7350704046119176
1101	155-159	0.9516319260522579
1101	160-164	1.1401523011072854
1101	165-169	1.1174134223072798
1101	170-174	1.0740789081210664
1101	175-179	1.2793747538415339
1101	180-184	0.7712697081144455
1101	185-189	0.9174345444748546
1101	190-194	0.8814334579390461
1101	195-199	1.1062703375201899
1101	200-204	0.9965389783344385
1101	205-209	1.6826069135934851
1101	210-214	0.8838640396664736
1101	215-219	1.047593532617416
1101	220-224	1.2676894685840239
1101	225-229	1.2376512176667873
1101	230-234	1.3918109123219438
1101	235-239	1.4149928873243454
1101	240-244	1.4192388123861868
1101	245-249	0.9664181701946077
1101	250-254	0.6758871592080915
1101	255-259	0.2953984543855839
1101	260-264	0.21361491269490074
1101	265-269	-0.2537835895386422
1101	270-274	1.0103694119327145
1101	275-279	0.8508081757875878
1101	280-284	0.9129673623894554
1101	285-289	0.7646756088941551
1101	290-294	-0.013024337926481167
1101	295-299	-0.34347887698062607
1101	300-301	0.5064579158353517
1102	1	0.3555221284106622
1102	2	0.07068269174281738
1102	3	0.036660203075626896
1102	4	0.13295647312165215
1102	5	0.040152447209692355
1102	6	0.027790863909217478
1102	7	-0.018608148273251857
1102	8	-0.0030800332910416728
1102	9	0.1084401652993563
1102	10-14	0.033887247728408454
1102	15-19	0.17623192582941272
1102	20-24	0.24012987711962097
1102	25-29	0.13680059720332594
1102	30-34	0.1057501131381855
1102	35-39	0.1261667710843568
1102	40-44	0.10735334770540561
1102	45-49	0.08927056813583079
1102	50-54	0.4349101653948608
1102	55-59	0.2543576248658752
1102	60-64	0.2797481968947153
1102	65-69	0.4718601963377296
1102	70-74	0.10970713587207115
1102	75-79	0.21570338937179656
1102	80-84	0.25906628533776654
1102	85-89	0.35922295773938373
1102	90-94	0.4806192667489668
1102	95-99	0.32255375765370076
1102	100-104	0.248521233005512
1102	105-109	0.2388296395706746
1102	110-114	0.21417676840405164
1102	115-119	0.2511388559306269
1102	120-124	0.508214275028088
1102	125-129	0.23686689869843036
1102	130-134	0.23799013968103822
1102	135-139	0.3924113048439324
1102	140-144	0.28912606126807816
1102	145-149	0.13399905076219198
1102	150-154	0.11124029181140571
1102	155-159	0.40731374686191657
1102	160-164	0.443869077752872
1102	165-169	0.228597017763974
1102	170-174	0.43764889633180815
1102	175-179	0.4207101023308546
1102	180-184	0.2046937628890646
1102	185-189	0.09359858757030537
1102	190-194	0.036696607763825995
1102	195-199	0.2923693103504128
1102	200-204	0.14146571790301365
1102	205-209	0.8229886029623401
1102	210-214	0.279579203540429
1102	215-219	0.49835290607014215
1102	220-224	0.5596705629200294
1102	225-229	0.7556448482720626
1102	230-234	0.40733783961571035
1102	235-239	0.40068610652480174
1102	240-244	0.5312732239286433
1102	245-249	0.37925854149655436
1102	250-254	0.41722527202146864
1102	255-259	0.12698346827796314
1102	260-264	0.12245233917167297
1102	265-269	0.42304634333887137
1102	270-274	1.1977575670925944
1102	275-279	0.3034201679242621
1102	280-284	0.6543258365640234
1102	285-289	0.8985316303045394
1102	290-294	0.7380977614687332
1102	295-299	0.6401373470163207
1102	300-301	0.5256094516558427
1103	1	0.35716392607959335
1103	2	0.07434321710688607
1103	3	0.038732273457547706
1103	4	0.1265745898690298
1103	5	0.049761777402402174
1103	6	0.166451469871447
1103	7	0.16104077718169663
1103	8	0.27899835481873936
1103	9	0.1859559304433347
1103	10-14	0.15434825674113029
1103	15-19	0.19371248119589524
1103	20-24	0.29618174195795177
1103	25-29	0.16310744704436786
1103	30-34	0.10070130085125584
1103	35-39	0.16126361981541493
1103	40-44	0.1462729673978984
1103	45-49	0.003652813527118326
1103	50-54	0.4049097421457475
1103	55-59	0.3627714301122964
1103	60-64	0.3143860151519746
1103	65-69	0.6085594577639881
1103	70-74	0.3195962193744961
1103	75-79	0.42692817306763686
1103	80-84	0.4145408448470391
1103	85-89	0.5254653730450372
1103	90-94	0.7193088476582687
1103	95-99	0.46692966027391947
1103	100-104	0.4623578875445915
1103	105-109	0.5151722797818721
1103	110-114	0.42797908607418833
1103	115-119	0.4609594064467899
1103	120-124	0.4955633167469813
1103	125-129	0.614334256161996
1103	130-134	0.6456773931738482
1103	135-139	0.7702052256269951
1103	140-144	0.45595543628281376
1103	145-149	0.6200449202031848
1103	150-154	0.6068579670026395
1103	155-159	0.9213283623976736
1103	160-164	0.9688270355875161
1103	165-169	0.8514893453021415
1103	170-174	1.0709930198972302
1103	175-179	0.8716747654232542
1103	180-184	0.672847568025368
1103	185-189	0.572814725425804
1103	190-194	0.40640731248894824
1103	195-199	0.5377178380058787
1103	200-204	0.6447784270752948
1103	205-209	1.1983311016637934
1103	210-214	0.8349214108580689
1103	215-219	0.8449850999426225
1103	220-224	1.042764451554536
1103	225-229	0.9804450443648491
1103	230-234	1.1802983816624177
1103	235-239	1.090336052786263
1103	240-244	1.1071634940448511
1103	245-249	0.7681329954564156
1103	250-254	0.2214812826653514
1103	255-259	0.8824740038722219
1103	260-264	0.25354946388775446
1103	265-269	0.8551386450408778
1103	270-274	1.0409410633624425
1103	275-279	0.9949991622851115
1103	280-284	0.45660849311035534
1103	285-289	1.2674578364890152
1103	290-294	0.27265460249493856
1103	295-299	-0.346720677570012
1103	300-301	0.7616812374150079
2116	1	0.4411539202964505
2116	2	0.06858352984249194
2116	3	0.015666668782621684
2116	4	0.15901993363715405
2116	5	0.047874966512566175
2116	6	0.15074612138197097
2116	7	0.09265425252566928
2116	8	0.27942057845742596
2116	9	0.2038959144734349
2116	10-14	0.12579863080266307
2116	15-19	0.20995766030642926
2116	20-24	0.3471475503941619
2116	25-29	-0.036077431083128886
2116	30-34	-0.04943172625784342
2116	35-39	0.14504365031525168
2116	40-44	0.115890646934659
2116	45-49	0.16313297901615442
2116	50-54	0.46360403369977377
2116	55-59	0.325898326356608
2116	60-64	0.31855105357268343
2116	65-69	0.6666435875623478
2116	70-74	0.42442457530486877
2116	75-79	0.45551938578003615
2116	80-84	0.4285910474419552
2116	85-89	0.49956772358762436
2116	90-94	0.7543657748460078
2116	95-99	0.6952379189134064
2116	100-104	0.49395309449528924
2116	105-109	0.6626795108101149
2116	110-114	0.626189099230487
2116	115-119	0.5589108938622118
2116	120-124	0.6639169852105624
2116	125-129	0.631176535732692
2116	130-134	0.7006862397854619
2116	135-139	0.7803701392731526
2116	140-144	0.7509643982330232
2116	145-149	0.7379098156536017
2116	150-154	0.7383736362441056
2116	155-159	0.8181044697364825
2116	160-164	0.9243349432222203
2116	165-169	0.7469015292383148
2116	170-174	0.921501514924266
2116	175-179	0.9120436396317224
2116	180-184	0.6025718520475607
2116	185-189	0.647457113117337
2116	190-194	0.6611229194264396
2116	195-199	0.7828955998229077
2116	200-204	0.3915848157680024
2116	205-209	1.3127032927224285
2116	210-214	0.7709191962358481
2116	215-219	-0.42929254933505234
2116	220-224	0.09213844037698848
2116	225-229	1.1087458548692908
2116	230-234	0.5870248932191089
2116	235-239	0.8972047997361017
2116	240-244	0.709126830507067
2116	245-249	0.5843137897724908
2116	250-254	0.22893690865471683
2116	255-259	0.7369719469749754
2116	260-264	1.036957601080303
2116	265-269	0.7050675284321102
2116	270-274	1.056706540524818
2116	275-279	1.1558437866107987
2116	280-284	0.932806672489555
2116	285-289	0.9082161269396174
2116	290-294	-0.7331022964876794
2116	295-299	-0.20797308878494647
2116	300-301	0.7314608195411019
1117	1	0.40524325545955264
1117	2	0.09470526888407704
1117	3	0.03372339853772388
1117	4	0.15401164250690869
1117	5	0.057465490127896146
1117	6	0.2048592152260298
1117	7	0.13330148570182132
1117	8	0.2294561895055054
1117	9	0.1143930193441065
1117	10-14	0.1960993896836598
1117	15-19	0.20066902730335556
1117	20-24	0.27241421719538295
1117	25-29	0.22856390554596828
1117	30-34	0.1121129241771257
1117	35-39	-0.1035546133161418
1117	40-44	-0.695262194494461
1117	45-49	-0.14493039858414392
1117	50-54	-0.49748004112549893
1117	55-59	0.22124684288148444
1117	60-64	0.5745814410745425
1117	65-69	0.6774751987454195
1117	70-74	0.25134236333180127
1117	75-79	-0.1283124798522337
1117	80-84	0.40579903749637936
1117	85-89	0.3832585671108717
1117	90-94	0.6830358905164502
1117	95-99	-0.250645703826585
1117	100-104	0.5047195987346313
1117	105-109	0.6374101939075061
1117	110-114	0.4677102567870577
1117	115-119	0.6629370743716336
1117	120-124	0.706262801846762
1117	125-129	0.6586013246986724
1117	130-134	0.792492981191856
1117	135-139	0.8667909557735243
1117	140-144	0.5981689313470397
1117	145-149	0.876552076530416
1117	150-154	0.8568587093779314
1117	155-159	1.039920694127325
1117	160-164	1.1638981446279075
1117	165-169	0.8411858113656621
1117	170-174	0.97079788931228
1117	175-179	1.2414079257887245
1117	180-184	0.7581350526701556
1117	185-189	0.7921819493508657
1117	190-194	0.7465937254669441
1117	195-199	0.7413202937932617
1117	200-204	0.7024526724013498
1117	205-209	1.5025932710817322
1117	210-214	0.638352575486195
1117	215-219	-0.4520960367087383
1117	220-224	-0.17614387094566197
1117	225-229	1.295719904660455
1117	230-234	0.5844948908699195
1117	235-239	0.9887883336499961
1117	240-244	1.0180978249469987
1117	245-249	0.40253792860409376
1117	250-254	-0.12346788704743261
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2108	260-264	0.5733666453808013
2108	265-269	0.247955750297308
2108	270-274	0.8489081463584327
2108	275-279	0.9830428969400948
2108	280-284	0.09438673544227427
2108	285-289	-0.07793574266093728
2108	290-294	0.9108006577184256
2108	295-299	0.1619823031092551
2108	300-301	-0.3805787317245759
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	175.0
13	879.0
14	3781.0
15	19266.0
16	30100.0
17	35087.0
18	64568.0
19	167846.0
20	369112.0
21	509197.0
22	493030.0
23	475601.0
24	488726.0
25	526299.0
26	576458.0
27	652841.0
28	769902.0
29	902327.0
30	1058394.0
31	1247203.0
32	1479388.0
33	1775358.0
34	2214407.0
35	2782287.0
36	2381293.0
37	268468.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	3.7667073830363322	89.57622060341608	3.42726089240641	3.229811121141178
2	88.63313514232199	4.7109072960054155	3.7062159719614276	2.949741589711173
3	3.183574327071928	4.128510296628213	3.5394680539778287	89.14844732232203
4	3.3743840385610713	4.187192667217672	89.19288544290002	3.245537851321235
5	3.555371023059046	4.1762347543631435	3.5566254293555435	88.71176879322226
6	3.4589735276211715	4.599773118332241	35.85907522783414	56.082178126212455
7	4.957149325406729	58.2809502075861	5.577919753763154	31.183980713244015
8	7.3591248598188015	71.20416006742693	15.384992583711535	6.051722489042735
9	9.735597588533484	16.671360323284347	70.32421996791933	3.268822120262834
10-14	38.36605182615899	21.127551608840868	24.47767791770628	16.028718647293864
15-19	4.28330818041369	40.003873109027865	44.259616488600585	11.453202221957865
20-24	19.942518127337273	42.00863518780717	30.451959996879534	7.596886687976023
25-29	37.59687476593271	4.224568791356208	49.11761795501191	9.060938487699174
30-34	27.455237262916558	13.878701502877231	38.7780714228881	19.887989811318114
35-39	3.991562303432071	88.44464660083106	3.542914042845232	4.020877052891627
40-44	37.63512171758286	4.064656869338811	20.939068250847047	37.361153162231275
45-49	20.70592906539733	21.025590147623404	53.61632739382319	4.652153393156073
50-54	31.692179061833674	29.02368573079145	27.368518393250675	11.9156168141242
55-59	37.628598804841076	9.21363601846258	36.018433552362	17.139331624334343
60-64	30.505706641537074	21.88670896918644	27.27954573904876	20.32803865022772
65-69	31.193296494219492	17.238876539207066	29.452224096056423	22.11560287051702
70-74	32.72684654956628	9.396787631346577	34.7872047447659	23.08916107432124
75-79	17.72268238717665	9.49515381898036	27.272171696084307	45.50999209775868
80-84	35.887392672453004	14.74160137404141	28.400857454089117	20.970148499416467
85-89	31.096426263846737	20.303138166892538	28.833967663790084	19.766467905470638
90-94	29.96034987568678	17.465755096867895	29.58723553473863	22.98665949270669
95-99	24.04912711204829	17.846466371155497	28.977841845801848	29.12656467099437
100-104	27.56811724241065	13.949171145856093	32.01704230174225	26.465669309991007
105-109	21.001482957065694	13.800178921881113	37.51494479979851	27.68339332125468
110-114	25.505140344479667	17.223961544671766	33.18023045309725	24.09066765775131
115-119	29.916881069065177	13.065586011192726	29.209578376938204	27.80795454280389
120-124	28.445827991264316	15.209804174421654	30.93737959059972	25.406988243714313
125-129	23.619097181179004	19.476258618937635	36.296268336128726	20.608375863754638
130-134	26.55099747954522	22.098533036081193	27.172060270014796	24.17840921435879
135-139	26.222115221016235	18.76626400015957	30.397166194491536	24.61445458433266
140-144	31.708790096617793	18.19445111819695	31.061931127392477	19.03482765779278
145-149	35.036670599109534	19.013907457759707	25.95429401581939	19.995127927311362
150-154	27.236107279066857	25.762289755541506	25.376771302053225	21.624831663338412
155-159	19.922527035359288	24.78926284573253	34.1758247659454	21.112385352962782
160-164	16.110520785652717	32.720519214742055	35.65200562517263	15.516954374432595
165-169	15.514302979774927	40.459963858582995	31.998252746709333	12.027480414932743
170-174	14.72167366421532	41.99022765609403	29.61106871586213	13.677029963828518
175-179	24.63824863970255	26.847297659819898	30.368670344482485	18.145783355995064
180-184	24.717355298423684	38.3169393828976	21.875510185717243	15.090195132961473
185-189	27.215449894772387	30.949082544054946	21.253633308623595	20.581834252549076
190-194	26.03305012739949	19.93178599771567	32.887088768364734	21.148075106520103
195-199	23.145414227455177	26.493366994138285	30.988200772998432	19.37301800540811
200-204	19.688883743165782	26.657204420003826	27.853345910037202	25.80056592679319
205-209	19.191588191414876	29.715344368617675	36.45006057541701	14.64300686455044
210-214	25.375395633044285	32.07283734684294	29.826665085077884	12.725101935034894
215-219	19.85322237448046	44.2975214271176	25.265481702242347	10.583774496159592
220-224	23.72737825499494	42.27770599829946	24.10152275991398	9.893392986791625
225-229	28.567796556752935	33.534406014004944	23.389320267890675	14.508477161351449
230-234	25.506921396253574	24.72059427743773	32.262333354288536	17.51015097202016
235-239	17.82517728266443	18.932374293381788	47.20468831999835	16.03776010395543
240-244	25.21733821616101	14.667739778239575	44.33924470473835	15.775677300861071
245-249	28.509781001775075	16.191548383938493	44.508721402838994	10.789949211447441
250-254	26.116995493527252	20.96189077369651	40.369058193930066	12.55205553884618
255-259	27.77652998580945	22.024069286772697	36.40533921895351	13.794061508464345
260-264	24.64218558047678	31.859067276940063	33.372588495689904	10.126158646893256
265-269	26.131071016270642	30.54285613606969	31.667509696886377	11.65856315077329
270-274	27.248063604715405	37.321268928213044	25.440981120421352	9.989686346650197
275-279	34.470041892151784	31.843265563971567	22.078789657093154	11.607902886783497
280-284	34.41144899095207	29.02038655906524	20.94007537673074	15.628089073251953
285-289	41.3894202892045	20.379806192010594	23.9803105293233	14.25046298946161
290-294	33.90966609981048	23.69667852888455	25.2137005687862	17.179954802518772
295-299	26.172250678361397	23.84038541703049	33.08581162687273	16.901552277735373
300-301	33.49941492433827	16.658784102156375	31.99228655986811	17.849514413637245
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	1.25
15	3.5
16	2.25
17	6.5
18	14.0
19	28.75
20	57.75
21	108.5
22	255.75
23	362.5
24	455.0
25	590.0
26	1017.5
27	1518.5
28	1896.0
29	2793.75
30	4375.5
31	6096.5
32	7831.25
33	11958.25
34	19400.25
35	32165.0
36	53263.0
37	109040.5
38	299490.25
39	671504.25
40	436447.5
41	416695.75
42	512135.5
43	503416.5
44	424355.25
45	325836.25
46	301616.0
47	320112.5
48	331293.0
49	647431.25
50	328513.0
51	113146.0
52	92226.0
53	84157.5
54	51263.0
55	37316.75
56	31070.0
57	25394.25
58	23557.0
59	28275.25
60	23777.25
61	15149.25
62	11218.25
63	12557.5
64	15831.0
65	10874.0
66	6263.75
67	4260.75
68	2853.75
69	2054.0
70	1501.25
71	981.75
72	598.25
73	367.25
74	220.75
75	133.5
76	96.75
77	61.75
78	36.5
79	26.75
80	19.5
81	17.0
82	13.75
83	12.75
84	10.0
85	11.75
86	7.75
87	5.5
88	7.5
89	9.75
90	14.75
91	8.75
92	5.75
93	2.75
94	0.75
95	1.75
96	2.75
97	3.5
98	2.25
99	8.0
100	61.000000000000156
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	1.0366994185930486E-6
110-114	0.0
115-119	0.0
120-124	2.073398837186097E-6
125-129	1.0366994185930486E-6
130-134	2.073398837186097E-6
135-139	3.1100982557791456E-6
140-144	0.0
145-149	2.073398837186097E-6
150-154	1.0366994185930486E-6
155-159	3.1100982557791456E-6
160-164	2.073398837186097E-6
165-169	5.183497092965243E-6
170-174	5.183497092965243E-6
175-179	6.220196511558291E-6
180-184	3.1100982557791456E-6
185-189	3.110098255779146E-5
190-194	1.4513791860302679E-5
195-199	1.2440393023116582E-5
200-204	1.5446821337036424E-4
205-209	5.494506918543157E-5
210-214	9.330294767337438E-6
215-219	3.255236174382173E-4
220-224	1.9282609185830704E-4
225-229	3.3900070987992687E-4
230-234	2.56064756392483E-4
235-239	1.834957970909696E-4
240-244	3.0478962906635627E-4
245-249	8.677174133623816E-4
250-254	5.173130098779312E-4
255-259	7.236161941779479E-4
260-264	4.924322238316981E-4
265-269	4.188265651115916E-4
270-274	4.7688173255280234E-4
275-279	0.0011362225627779812
280-284	0.001066763701732247
285-289	0.0015716363185870616
290-294	0.0010989013837086315
295-299	0.0022517111371841013
300-301	0.00245179412497256
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
301	1.9291995E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	4.061277957230645
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.4501516292041	3.145465935958578
2	11.610495100154747	0.9430689564558578
3	4.372821499588289	0.5327773070154651
4	1.7936492987616766	0.29138033440252004
5	1.0658428449143755	0.21643420259613777
6	0.6133250806259261	0.1494530178337669
7	0.39926622916167676	0.11350717948926423
8	0.2434295699638661	0.07909081173059077
9	0.14900597217165218	0.05446392032488086
>10	1.1181212034374788	0.8955541958248698
>50	0.21402372904210354	0.6379864867064089
>100	0.8860092858266194	7.162251207137323
>500	0.03238979081645063	0.916806035090594
>1k	0.03541697614996311	3.0361296987785646
>5k	0.005526026159090434	1.607807733734657
>10k+	0.01052576402187037	80.21782297692059
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG	3363626	17.43534559282231	No Hit
AGCTCTCATGGCGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTG	2687491	13.930601785870254	No Hit
AGCTCTCTAAGTGTAAATCGTTATTGTTGCGGTTAAAAAGCTCGTAGTTG	1765961	9.153853709789992	No Hit
AGCTCCAATAGCGTATATTTAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG	865855	4.48815687542942	No Hit
AGCTCCAAGAGCATATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG	821374	4.257589741237233	No Hit
AGCTCTAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG	375256	1.9451383851177653	No Hit
AGCTCCATTAATATAGATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTG	329916	1.710118626922721	No Hit
AGCTCCAATAGGATATATCAAAGTTGTTGCAGTTAAAAAGCTCGTAGTCG	246509	1.2777786848897692	No Hit
AGCTCCAATAAGGAAAACTAAAGTTGCTGTGTTTAAAAAGCTCGTAGTTG	237722	1.2322312959338835	No Hit
AGCTCCAATAGGATATATCAAAGTTGTTGCAGTTGAAAAGCTCGTAGTCG	234133	1.2136277248672314	No Hit
AGCTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTG	221556	1.1484348819290073	No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGTGGTTAAAAAGCTCGTAGTTG	189395	0.9817284319221522	No Hit
AGCTCCAATAGCGTATATTAAAATTGTTGCAGTTAAAAAGCTCGTAGTTG	185556	0.9618289865822586	No Hit
AGCTCCAATAGCGTATACTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG	184268	0.9551526423265193	No Hit
AGCTCCAGTAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG	176576	0.9152811826874307	No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGCGGTTAAAAAGCTCGTAGTTG	170730	0.8849784586819559	No Hit
AGCTCTGATAGTATATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG	169157	0.8768248177547215	No Hit
AGCTCTCCTAGTGTATCTCGTTATTGCTGCGGTTAAAAAGCTCGTAGTTG	166380	0.862430246327557	No Hit
AGCTCCAGTAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTG	146402	0.7588743414042974	No Hit
AGCTCTCAATGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTG	137790	0.7142340644396808	No Hit
AGCTCATGTAGTGTATATTAAAATTGTTGCAGTTAAAAAGCTCGTAGTTG	130140	0.6745803116784967	No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTCG	122065	0.6327235726528024	No Hit
AGCTCCTCTAGTGTACATCGATATTGCTGCGGTTAAAAAGCTCGTAGTTT	115923	0.6008865335078099	No Hit
AGCTCTCAAAGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTG	107518	0.557319240441437	No Hit
AGCTCCAAGAGCGTATATAAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTG	105184	0.5452209582264561	No Hit
AGCTCCAAGAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG	90391	0.46854148573022125	No Hit
AGCTCCAATAGCTTTGTTGAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTG	82892	0.4296704410300749	No Hit
AGCTCTCAAGGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTG	79948	0.41441022558838525	No Hit
AGCTCCAAAAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG	77710	0.4028095590943291	No Hit
AACTCCAGTAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTG	73445	0.38070194399283225	No Hit
AGCTCCAATAGCGTACGTTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTG	73251	0.379696345556797	No Hit
AGCTCCAGTAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTCG	70723	0.3665924649057809	No Hit
AGCTCTCAAGGTGTATATCCCTATTGCTGCGGTTAAAAAGCTCGTAGTTG	62195	0.3223876016969733	No Hit
AGCTCCAATAGCATATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG	54014	0.27998141197942467	No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGCGGTTAAAACGTTCGTAGTTG	53354	0.27656030389806757	No Hit
AGCTCTTCTAGCATATATTAAAGTTGCTGCAGTTAAAACGCTCGTAGTTT	51233	0.2655661065638883	No Hit
AGCTCTTCTAGCGTATATCGAAGTTGATGCAGTTAAAACGCTCGTAGTCG	49442	0.25628246327038756	No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGTTCGTAGTTG	45062	0.2335787460031998	No Hit
AGCTCCACTAGTGTAAATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTG	40065	0.20767681102965246	No Hit
AGCTCCAATAGTGTATATTAAAGTTGCTGCGGTTAAAAAGCTCGTAGTTG	39736	0.20597144048606686	No Hit
AGCTCCAGTAGCGTATATTAAAGTTGTTGCAGTTAAAACGCTCGTAGTCG	39425	0.2043593728901547	No Hit
AGCTCCAAGAGTGTATATTAAAGTTGTTGCGGTTAAAAAGCTCGTAGTTG	37406	0.19389389225945788	No Hit
AGCTCCAATAGCGTATACTTAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG	36669	0.19007365490194247	No Hit
AGCTCCAATAGCGTAACTTAATGTTGTTGCAGTTAAAAAGCTCGTAGTTG	35742	0.1852685530967637	No Hit
AGCTCTCATACTGCATTGAATTGCTGTTGCGGTTAAAAAGTTCGTAGTTG	35467	0.18384309139619828	No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAACGCTCGTAGTCG	35435	0.1836772194892234	No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGCGGTTAAAACGCTCGTAGTTG	32456	0.1682355816492799	No Hit
AGCTCCAATAGCGTATATTTAAGTTGCTGCAGTTAAAAAGCTCGTAGTCG	31344	0.16247153288190258	No Hit
AGCTCCAATAGCGTATATTAATGTTGTTGCAGTTAAAAAGCTCGTAGTTG	30689	0.15907634228601034	No Hit
AGCTCCAATAGCGTATACTAATTTTGTTGCAGTTAAAAAGCTCGTAGTTG	29923	0.15510578351279897	No Hit
AGCTCCAATAGCGTATGTTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG	29220	0.1514617850564444	No Hit
AGCTCCAATAGTGTATATTAAAGTTGCTGTGATTAAAACGCTCGTAGTTG	27156	0.14076304705656414	No Hit
AGCTCCAATAGCATATACTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG	26979	0.1398455680711093	No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGAGGTTAAAAAGCTCGTAGTTG	26542	0.13758037984148347	No Hit
AGCTCCAAGAGCATATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTG	23103	0.119754333338776	No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAACGCTCGTAGTTG	22602	0.11715740129520041	No Hit
AGCTCTCATAGCGTATATCATCATTGCTGCGGTTAAAAAGCTCGTAGTTG	22040	0.11424427592895395	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0014410121918443375	0.0	0.0
2	0.0014410121918443375	0.0	0.0
3	0.0014565626831232333	0.0	0.0
4	0.0014669296773091637	0.0	0.0
5	0.001518764648238816	0.0	0.0
6	0.001518764648238816	0.0	0.0
7	0.001534315139517712	0.0	0.0
8	0.0015498656307966075	0.0	0.0
9	0.0015498656307966075	0.0	0.0
10-14	0.0016276180871910863	0.0	0.0
15-19	0.001748911919166473	0.0	0.0
20-24	0.0019344811150946288	0.0	0.0
25-29	0.002082729131953435	0.0	0.0
30-34	0.002496372199972061	0.0	0.0
35-39	0.002896538175548978	0.0	0.0
40-44	0.0033744566075203735	0.0	0.0
45-49	0.003979889067978713	0.0	0.0
50-54	0.004681734574366207	0.0	0.0
55-59	0.005628241143541661	0.0	0.0
60-64	0.006898197931318145	0.0	0.0
65-69	0.008412815781882589	0.0	0.0
70-74	0.01013373681674705	0.0	5.183497092965243E-6
75-79	0.012648769606253784	0.0	5.183497092965243E-6
80-84	0.015573298666104777	0.0	5.183497092965243E-6
85-89	0.019178939243971398	0.0	5.183497092965243E-6
90-94	0.023714499200315986	0.0	5.183497092965243E-6
95-99	0.029150950951417932	0.0	5.183497092965243E-6
100-104	0.036068846171689345	0.0	1.0366994185930486E-5
105-109	0.0447553506000805	0.0	1.555049127889573E-5
110-114	0.055542208050541164	0.0	1.555049127889573E-5
115-119	0.06756792130622054	0.0	1.555049127889573E-5
120-124	0.08097866498514021	0.0	2.073398837186097E-5
125-129	0.09643689001578115	0.0	2.5917485464826212E-5
130-134	0.11324904448710463	0.0	2.5917485464826212E-5
135-139	0.13103569641190554	0.0	2.5917485464826212E-5
140-144	0.14998863518262368	0.0	2.5917485464826212E-5
145-149	0.1697014746271705	4.146797674372194E-6	4.250467616231499E-5
150-154	0.18987357191415402	2.5917485464826212E-5	4.7688173255280234E-5
155-159	0.21026855957613505	2.5917485464826212E-5	5.909186685980377E-5
160-164	0.23173653113635995	2.5917485464826212E-5	7.25689593015134E-5
165-169	0.25347715464367476	2.5917485464826212E-5	7.567905755729254E-5
170-174	0.2752893104108725	2.6954184883419263E-5	7.87891558130717E-5
175-179	0.2985829096472397	3.110098255779146E-5	8.293595348744388E-5
180-184	0.3223202162347647	3.110098255779146E-5	8.293595348744388E-5
185-189	0.3462762663996129	3.110098255779146E-5	8.293595348744388E-5
190-194	0.3706480330313169	3.110098255779146E-5	8.811945058040913E-5
195-199	0.39404426551012484	3.4211080813570606E-5	8.811945058040913E-5
200-204	0.41795677429939204	3.62844796507567E-5	8.811945058040913E-5
205-209	0.44290287240899656	4.6651473836687183E-5	9.64130459291535E-5
210-214	0.4679951451366227	6.738546220854815E-5	9.848644476633962E-5
215-219	0.49297856442529664	1.368443232542824E-4	9.848644476633962E-5
220-224	0.5179951580953654	1.555049127889573E-4	1.0263324244071183E-4
225-229	0.5435228445788007	1.575783116261434E-4	1.9075269302112096E-4
230-234	0.5690691916517706	1.6068840988192251E-4	2.498445598809247E-4
235-239	0.5950924204572934	1.6068840988192251E-4	2.539913575552969E-4
240-244	0.62137793421572	1.6068840988192251E-4	2.5710145581107603E-4
245-249	0.648121669117165	1.6068840988192251E-4	2.6124825348544824E-4
250-254	0.675654332276159	1.6068840988192251E-4	2.6954184883419265E-4
255-259	0.7034637941799177	1.6068840988192251E-4	2.799088430201231E-4
260-264	0.7313002102685595	1.6068840988192251E-4	2.882024383688675E-4
265-269	0.7588940386932508	1.6068840988192251E-4	2.923492360432397E-4
270-274	0.7869730424458435	1.627618087191086E-4	2.9545933429901884E-4
275-279	0.8151671198339001	1.6587190697488777E-4	2.9753273313620494E-4
280-284	0.8442859331033417	1.6587190697488777E-4	3.0582632848494935E-4
285-289	0.8736390404413852	1.6587190697488777E-4	3.151566232522867E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCTCCA	981715	0.0	294.25195	1
CCAATAG	730385	0.0	294.11417	5
TCCAATA	758560	0.0	293.8476	4
TAGCGTA	757810	0.0	293.52332	9
CTCCAAT	760205	0.0	293.46005	3
AGCTCTC	540675	0.0	293.3946	1
ATAGCGT	691360	0.0	293.08282	8
GCTCCAA	891105	0.0	293.05875	2
AATAGCG	687385	0.0	292.91296	7
CTCTAAG	186465	0.0	291.24924	5
CTAAGTG	186630	0.0	290.7625	7
TCTCATG	286275	0.0	290.4337	4
GCTCTCA	340270	0.0	290.42114	2
CTCATGG	288125	0.0	288.39484	5
CTCTCTA	188935	0.0	287.99594	3
CTCTCAT	297150	0.0	287.85577	3
ATGGCGT	291210	0.0	285.18768	8
TAAGTGT	190450	0.0	285.1396	8
CAATAGC	688900	0.0	283.96353	6
TCCAGTA	60460	0.0	282.6542	4
>>END_MODULE