FastQCFastQC Report
Tue 25 Aug 2015
000000000-AHV8M l01n01.33100000033c80.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AHV8M l01n01.33100000033c80.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19291995
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG336362617.43534559282231No Hit
AGCTCTCATGGCGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTG268749113.930601785870254No Hit
AGCTCTCTAAGTGTAAATCGTTATTGTTGCGGTTAAAAAGCTCGTAGTTG17659619.153853709789992No Hit
AGCTCCAATAGCGTATATTTAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG8658554.48815687542942No Hit
AGCTCCAAGAGCATATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG8213744.257589741237233No Hit
AGCTCTAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG3752561.9451383851177653No Hit
AGCTCCATTAATATAGATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTG3299161.710118626922721No Hit
AGCTCCAATAGGATATATCAAAGTTGTTGCAGTTAAAAAGCTCGTAGTCG2465091.2777786848897692No Hit
AGCTCCAATAAGGAAAACTAAAGTTGCTGTGTTTAAAAAGCTCGTAGTTG2377221.2322312959338835No Hit
AGCTCCAATAGGATATATCAAAGTTGTTGCAGTTGAAAAGCTCGTAGTCG2341331.2136277248672314No Hit
AGCTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTG2215561.1484348819290073No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGTGGTTAAAAAGCTCGTAGTTG1893950.9817284319221522No Hit
AGCTCCAATAGCGTATATTAAAATTGTTGCAGTTAAAAAGCTCGTAGTTG1855560.9618289865822586No Hit
AGCTCCAATAGCGTATACTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG1842680.9551526423265193No Hit
AGCTCCAGTAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG1765760.9152811826874307No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGCGGTTAAAAAGCTCGTAGTTG1707300.8849784586819559No Hit
AGCTCTGATAGTATATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG1691570.8768248177547215No Hit
AGCTCTCCTAGTGTATCTCGTTATTGCTGCGGTTAAAAAGCTCGTAGTTG1663800.862430246327557No Hit
AGCTCCAGTAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTG1464020.7588743414042974No Hit
AGCTCTCAATGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTG1377900.7142340644396808No Hit
AGCTCATGTAGTGTATATTAAAATTGTTGCAGTTAAAAAGCTCGTAGTTG1301400.6745803116784967No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTCG1220650.6327235726528024No Hit
AGCTCCTCTAGTGTACATCGATATTGCTGCGGTTAAAAAGCTCGTAGTTT1159230.6008865335078099No Hit
AGCTCTCAAAGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTG1075180.557319240441437No Hit
AGCTCCAAGAGCGTATATAAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTG1051840.5452209582264561No Hit
AGCTCCAAGAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG903910.46854148573022125No Hit
AGCTCCAATAGCTTTGTTGAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTG828920.4296704410300749No Hit
AGCTCTCAAGGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTG799480.41441022558838525No Hit
AGCTCCAAAAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG777100.4028095590943291No Hit
AACTCCAGTAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTG734450.38070194399283225No Hit
AGCTCCAATAGCGTACGTTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTG732510.379696345556797No Hit
AGCTCCAGTAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTCG707230.3665924649057809No Hit
AGCTCTCAAGGTGTATATCCCTATTGCTGCGGTTAAAAAGCTCGTAGTTG621950.3223876016969733No Hit
AGCTCCAATAGCATATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG540140.27998141197942467No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGCGGTTAAAACGTTCGTAGTTG533540.27656030389806757No Hit
AGCTCTTCTAGCATATATTAAAGTTGCTGCAGTTAAAACGCTCGTAGTTT512330.2655661065638883No Hit
AGCTCTTCTAGCGTATATCGAAGTTGATGCAGTTAAAACGCTCGTAGTCG494420.25628246327038756No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGTTCGTAGTTG450620.2335787460031998No Hit
AGCTCCACTAGTGTAAATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTG400650.20767681102965246No Hit
AGCTCCAATAGTGTATATTAAAGTTGCTGCGGTTAAAAAGCTCGTAGTTG397360.20597144048606686No Hit
AGCTCCAGTAGCGTATATTAAAGTTGTTGCAGTTAAAACGCTCGTAGTCG394250.2043593728901547No Hit
AGCTCCAAGAGTGTATATTAAAGTTGTTGCGGTTAAAAAGCTCGTAGTTG374060.19389389225945788No Hit
AGCTCCAATAGCGTATACTTAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG366690.19007365490194247No Hit
AGCTCCAATAGCGTAACTTAATGTTGTTGCAGTTAAAAAGCTCGTAGTTG357420.1852685530967637No Hit
AGCTCTCATACTGCATTGAATTGCTGTTGCGGTTAAAAAGTTCGTAGTTG354670.18384309139619828No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAACGCTCGTAGTCG354350.1836772194892234No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGCGGTTAAAACGCTCGTAGTTG324560.1682355816492799No Hit
AGCTCCAATAGCGTATATTTAAGTTGCTGCAGTTAAAAAGCTCGTAGTCG313440.16247153288190258No Hit
AGCTCCAATAGCGTATATTAATGTTGTTGCAGTTAAAAAGCTCGTAGTTG306890.15907634228601034No Hit
AGCTCCAATAGCGTATACTAATTTTGTTGCAGTTAAAAAGCTCGTAGTTG299230.15510578351279897No Hit
AGCTCCAATAGCGTATGTTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG292200.1514617850564444No Hit
AGCTCCAATAGTGTATATTAAAGTTGCTGTGATTAAAACGCTCGTAGTTG271560.14076304705656414No Hit
AGCTCCAATAGCATATACTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG269790.1398455680711093No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGAGGTTAAAAAGCTCGTAGTTG265420.13758037984148347No Hit
AGCTCCAAGAGCATATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTG231030.119754333338776No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAACGCTCGTAGTTG226020.11715740129520041No Hit
AGCTCTCATAGCGTATATCATCATTGCTGCGGTTAAAAAGCTCGTAGTTG220400.11424427592895395No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTCCA9817150.0294.251951
CCAATAG7303850.0294.114175
TCCAATA7585600.0293.84764
TAGCGTA7578100.0293.523329
CTCCAAT7602050.0293.460053
AGCTCTC5406750.0293.39461
ATAGCGT6913600.0293.082828
GCTCCAA8911050.0293.058752
AATAGCG6873850.0292.912967
CTCTAAG1864650.0291.249245
CTAAGTG1866300.0290.76257
TCTCATG2862750.0290.43374
GCTCTCA3402700.0290.421142
CTCATGG2881250.0288.394845
CTCTCTA1889350.0287.995943
CTCTCAT2971500.0287.855773
ATGGCGT2912100.0285.187688
TAAGTGT1904500.0285.13968
CAATAGC6889000.0283.963536
TCCAGTA604600.0282.65424
Produced by FastQC (version 0.11.2)