FastQCFastQC Report
Tue 22 Sep 2015
000000000-AHV8J l01n03.33300000034811.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AHV8J l01n03.33300000034811.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21106207
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAAGACATTCTTGGCAAATGCTTTCGCTTTAGGGCGTCCTTCTGCGATC4536732.1494766918565706No Hit
TGAAAACGTCCTTGGCAAATGCTTTCGCAGTAGTTAGTCTTCAATAAATC1000460.47401221830146933No Hit
TGAAAACATCCTTGGTAAATGCTTTCGCAGTCGTTCGTCGTGGGAAAATC896050.42454335826423006No Hit
TGAAAACATCCTTGGCAAATGCTTTCGCAGTAGTTAGTCTTCAATAAATC644110.3051756291407547No Hit
TGAAAACATCCTTGGCAAATGCTTTCGCATTAGTTAGTCTTCAGTAAATC639990.30322359673625865No Hit
TGAAAACATCCTTGGTAAATGCTTTCGCAGTCGTTCGTCGTGAAAAAATC614220.2910139183227No Hit
TGAAAACATTCTTGGCAAATGCTTTCGCTGTTGGACGTCCCGCTGCGGTC604280.286304403249717No Hit
TGAAAACGTCCTTGGCAAATGCTTTCGCAGTTGTTAGTCTTCCGTAAATC367280.17401516056390426No Hit
TGAAAACATCCTTGGTAAATGCTTTCGCAGTCGTTCGTCGTGGAAAAATC318310.15081345501823232No Hit
TGAAAACATCCTTGGCAAATGCTTTCGCATTTGTTAGTCTTTGACAAATC315830.14963844522135125No Hit
CGAAGACATTCTTGGCAAATGCTTTCGCTTTAGGGCGTCTTTCTGCGATC274810.13020340414551984No Hit
TGAAAACATCCTTGGCAAATGCTTTCGCATTAGTTCGTCTTTAATAAATC273740.12969644427347843No Hit
CGAAGACATTCTTGGCAAATGCTTTCGCTTTAGTGCGTCCTTCTGCGATC266440.1262377460810462No Hit
TGAAAACATCCTTGGCAAATGCTTTCGCATACGTTAGTCTTTAACAAATC238020.1127725128442074No Hit
TGAAAACATCTTTGGCAAATGCTTTCGCATTAGTTAGTCCTTAATAAATC237460.11250718805136328No Hit
TGAAAACATTCTTGGCAAACGCTTTCGCATCAGTTAGTCTTTGTCAAATC231160.109522284131867No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACATTC2335550.0353.4735
TCCTTGG4652750.0342.386729
ATTCTTG4677200.0338.775058
ATCCTTG4235950.0333.713938
TTCTTGG4912250.0331.027449
TGAAAAC5248950.0318.999021
ACATTCT4987400.0315.22416
GAAGACA2848050.0307.752442
CGAAGAC1563300.0307.1721
AAGACAT2832250.0303.969823
CCCTTGG142150.0303.60679
AGACATT2606400.0300.33364
CATCCTT4695850.0297.14697
ACATCCT4766100.0295.313756
GACATCC792950.0293.774875
TGAAGAC1139350.0293.505521
AACATCC3820800.0286.784765
TCCTAGG84800.0285.792889
TCCCTGG138950.0285.27419
AACGTCC706650.0284.747385
Produced by FastQC (version 0.11.2)