FastQCFastQC Report
Tue 22 Sep 2015
000000000-AHV8J l01n02.33200000034814.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AHV8J l01n02.33200000034814.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21106207
Sequences flagged as poor quality0
Sequence length12
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCTGGTGAT9073094.2987780798321555No Hit
AATCAGTCTCGT8687204.115945607848913No Hit
GTATGCGCTGTA8465914.011099673190924No Hit
TGAGTCACTGGT8318023.941030238166431No Hit
CCTCGTTCGACT8297883.931488021509502No Hit
GAACCAAAGGAT8235243.9018095482527957No Hit
CTAACCTCCGCT8143143.8581730957153977No Hit
GGATGGTGTTGC8029083.804132120944327No Hit
CAGGCGTATTGG7998143.78947292613969No Hit
TCCGACACAATT7969773.7760313826164973No Hit
TACACGATCTAC7922773.753763051788509No Hit
GACTTGGTATTC7899953.7429510664801118No Hit
GCACACACGTTA7851733.7201047066391415No Hit
TTACTGTGCGAT7530343.567831965260267No Hit
AGCTGTTGTTTG7461323.5351306845422292No Hit
GCTGATGAGCTG7151183.3881881287338835No Hit
GTACATACCGGT7064803.347261779437679No Hit
AATCCGTACAGC6906843.2724212360847216No Hit
GGAGACAAGGGA6873913.2568191906769415No Hit
AGGCTACACGAC6608123.130889410873304No Hit
CCAGTGTATGCA3452361.6357083961130485No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGT550.0091610646.00000055
GTGCAGC1101.8156243E-76.00000056
GGCAGAA550.0091610646.00000054
TCCGCGT550.0091610646.00000051
GGACTGT1054.8314723E-76.00000051
TCGGATT1054.8314723E-76.00000054
AAGCAGT1950.06.00000051
GCTTAGC1101.8156243E-76.00000054
CTGCAGT1950.06.00000053
CGAATGG550.0091610646.00000055
CCTGGCG2900.06.00000054
AGTAGCG550.0091610646.00000055
GGCTGGG1054.8314723E-76.00000051
GCGCTCT2100.06.00000055
CGTCCGG550.0091610646.00000055
GCATGCT1054.8314723E-76.00000051
GGTTAGC550.0091610646.00000054
GTGCGAA2350.06.00000056
ATCGCTG550.0091610646.00000053
GCATCTG1054.8314723E-76.00000051
Produced by FastQC (version 0.11.2)