##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AHV8J l01n01.33100000034817.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	21106207
Sequences flagged as poor quality	0
Sequence length	301
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.34613756038686	34.0	34.0	34.0	33.0	34.0
2	33.65682142698591	34.0	34.0	34.0	34.0	34.0
3	33.767324939057026	34.0	34.0	34.0	34.0	34.0
4	33.764376706814254	34.0	34.0	34.0	34.0	34.0
5	33.82223594225149	34.0	34.0	34.0	34.0	34.0
6	37.30249982860492	38.0	38.0	38.0	37.0	38.0
7	37.43609204628761	38.0	38.0	38.0	37.0	38.0
8	37.18048899075045	38.0	38.0	38.0	36.0	38.0
9	36.98364438480112	38.0	38.0	38.0	36.0	38.0
10-14	37.16241203357856	38.0	37.6	38.0	36.2	38.0
15-19	37.1819407058786	38.0	38.0	38.0	36.4	38.0
20-24	36.947040356422164	38.0	37.0	38.0	35.4	38.0
25-29	37.22112709308688	38.0	37.8	38.0	36.4	38.0
30-34	37.10906122544899	38.0	37.8	38.0	36.2	38.0
35-39	37.19853271599203	38.0	38.0	38.0	36.8	38.0
40-44	37.30781200051719	38.0	38.0	38.0	36.6	38.0
45-49	36.99857583127086	38.0	37.6	38.0	35.8	38.0
50-54	36.161019220554415	38.0	37.2	38.0	32.6	38.0
55-59	35.74050889390026	38.0	36.8	38.0	30.6	38.0
60-64	34.57468045300607	38.0	35.8	38.0	24.2	38.0
65-69	34.47266019896422	38.0	35.8	38.0	23.2	38.0
70-74	34.55470910524094	38.0	35.4	38.0	24.2	38.0
75-79	34.18394038303519	38.0	34.6	38.0	22.6	38.0
80-84	33.95576723946657	38.0	34.0	38.0	21.4	38.0
85-89	33.90516559417806	38.0	34.0	38.0	21.6	38.0
90-94	33.80688487514597	38.0	34.2	38.0	21.2	38.0
95-99	33.58893916846357	38.0	34.0	38.0	20.6	38.0
100-104	33.45904513302651	38.0	34.0	38.0	20.0	38.0
105-109	33.5231531084671	38.0	34.0	38.0	19.4	38.0
110-114	33.362520219762835	38.0	34.0	38.0	19.4	38.0
115-119	33.142502174834156	38.0	33.4	38.0	17.2	38.0
120-124	32.96537622321244	37.6	33.0	38.0	14.6	38.0
125-129	32.92906889428309	37.4	32.8	38.0	13.2	38.0
130-134	32.729694966035346	37.0	32.0	38.0	12.0	38.0
135-139	32.68342939117389	37.0	32.0	38.0	11.8	38.0
140-144	32.576341537823446	37.0	31.6	38.0	11.0	38.0
145-149	32.45286505528919	37.0	31.0	38.0	11.0	38.0
150-154	32.00302449416894	37.0	29.4	38.0	11.0	38.0
155-159	32.0257890202631	37.0	29.6	38.0	11.0	38.0
160-164	32.086609062443095	37.0	29.8	38.0	11.0	38.0
165-169	31.982293388859496	37.0	29.6	38.0	11.0	38.0
170-174	32.16008191334426	37.0	30.2	38.0	11.0	38.0
175-179	31.526527461803063	37.0	27.6	38.0	11.0	38.0
180-184	31.406794787902914	37.0	27.4	38.0	11.0	38.0
185-189	31.2437592884406	37.0	27.0	38.0	11.0	38.0
190-194	31.09983090756193	36.8	26.6	38.0	11.0	38.0
195-199	30.760230021433983	36.6	25.2	38.0	11.0	38.0
200-204	30.256230567624016	35.8	24.0	38.0	11.0	38.0
205-209	30.121875228457675	35.2	23.8	38.0	11.0	38.0
210-214	29.82057770967564	35.0	23.8	38.0	11.0	38.0
215-219	29.399957339563663	34.0	22.4	38.0	11.0	38.0
220-224	29.385661251213918	34.0	22.8	38.0	11.0	38.0
225-229	28.95275406898075	34.0	21.6	38.0	11.0	38.0
230-234	28.55340580143083	34.0	20.8	37.0	10.8	38.0
235-239	28.587319313223826	34.0	21.4	37.0	10.4	38.0
240-244	28.43890416691166	33.8	21.0	37.2	10.4	38.0
245-249	28.1116259970349	33.6	20.6	37.0	10.0	38.0
250-254	26.785127540917227	29.8	17.8	37.0	9.6	37.8
255-259	26.465689216447082	28.8	17.2	36.8	9.6	38.0
260-264	24.713217907888424	25.8	15.2	35.2	8.8	37.4
265-269	24.199007552612365	25.0	14.8	34.6	9.0	37.0
270-274	23.149783577883035	23.6	13.2	33.2	8.6	37.0
275-279	22.416958167803436	22.6	12.6	32.4	8.6	37.0
280-284	22.458712861102896	22.8	11.2	32.6	8.6	36.8
285-289	23.389561895228262	24.2	10.8	33.6	8.8	37.2
290-294	22.21960157976277	22.4	10.0	32.6	8.6	36.2
295-299	21.539101440633083	21.4	9.6	31.6	8.4	36.0
300-301	16.503209411335728	15.5	8.0	22.5	7.5	29.5
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	-6.648702260524825
1101	2	-1.3389484353121546
1101	3	-0.22639455198169856
1101	4	-0.051962199816465215
1101	5	-0.018526938090076328
1101	6	0.05699764920669992
1101	7	0.08507409896854057
1101	8	0.13953692839335474
1101	9	0.16702761124653875
1101	10-14	0.21645425272930652
1101	15-19	0.1652465802863219
1101	20-24	0.2841683421432961
1101	25-29	0.16930601925135846
1101	30-34	0.1531004957253046
1101	35-39	0.21087871216698773
1101	40-44	0.16308489112375213
1101	45-49	0.22745473612095424
1101	50-54	0.4706525946185991
1101	55-59	0.4278064856323809
1101	60-64	0.43513791445107586
1101	65-69	0.44012329636884573
1101	70-74	0.4784191531819033
1101	75-79	0.37955652143273255
1101	80-84	0.43786240624014283
1101	85-89	0.5150973262052503
1101	90-94	0.4207705422911019
1101	95-99	0.4324645347391396
1101	100-104	0.36774826884747824
1101	105-109	0.35619275820871366
1101	110-114	0.3882238266808926
1101	115-119	0.48374588340544733
1101	120-124	0.4510537306084075
1101	125-129	0.4644727430994422
1101	130-134	0.4503230443411823
1101	135-139	0.5163692648409324
1101	140-144	0.5012201041727238
1101	145-149	0.504206868565575
1101	150-154	0.7790314833557872
1101	155-159	0.4653654294372913
1101	160-164	0.6115747726109717
1101	165-169	0.5951557781871735
1101	170-174	0.4759059155643328
1101	175-179	0.6675698262159422
1101	180-184	0.6239141007159574
1101	185-189	0.5698330625997663
1101	190-194	0.6320497101549414
1101	195-199	0.6459256892102623
1101	200-204	0.5664641146095271
1101	205-209	0.6216948898042709
1101	210-214	0.8197624802759336
1101	215-219	0.6453574726343092
1101	220-224	0.6855882436277945
1101	225-229	0.41520743265571625
1101	230-234	0.6819823527353783
1101	235-239	0.6324675559504982
1101	240-244	0.7278628475319842
1101	245-249	0.8153500003825833
1101	250-254	0.8473133725019046
1101	255-259	0.5594876832781672
1101	260-264	-0.031101254733805206
1101	265-269	-0.05722043099358132
1101	270-274	-0.5599657674497109
1101	275-279	-0.349284651954445
1101	280-284	-0.030766255941166776
1101	285-289	0.2488492507301352
1101	290-294	0.05430440627517896
1101	295-299	-0.25798502127166145
1101	300-301	0.014608191682903282
1102	1	0.1847451841739698
1102	2	0.091090094810653
1102	3	0.008901739353106564
1102	4	0.026812856332220747
1102	5	-0.032874654368967526
1102	6	0.006658620800941151
1102	7	0.08436621536935718
1102	8	0.043817984159836953
1102	9	0.007022847249757547
1102	10-14	0.17326437667827577
1102	15-19	0.2135107319717804
1102	20-24	0.28052223202190163
1102	25-29	0.14686542360961852
1102	30-34	0.16502124789931827
1102	35-39	0.21181456070903693
1102	40-44	0.11514303189665753
1102	45-49	0.24128428684526426
1102	50-54	0.48714466136401313
1102	55-59	0.48591665759627745
1102	60-64	0.5531361532663297
1102	65-69	0.5823875743098768
1102	70-74	0.5463394891875311
1102	75-79	0.47245535004228145
1102	80-84	0.5567316139554208
1102	85-89	0.6823884787473773
1102	90-94	0.5687694532511571
1102	95-99	0.5621754489093078
1102	100-104	0.5936088223469014
1102	105-109	0.5199777626850377
1102	110-114	0.4860328650288466
1102	115-119	0.5980280833400755
1102	120-124	0.5785988089030027
1102	125-129	0.5015024457433341
1102	130-134	0.5821147677798066
1102	135-139	0.7282014005973991
1102	140-144	0.7524850587823622
1102	145-149	0.7659197335092642
1102	150-154	0.9203262520611659
1102	155-159	0.43199954608448365
1102	160-164	0.7164767605170113
1102	165-169	0.7978274703040462
1102	170-174	0.6499722037138866
1102	175-179	0.9288006117181276
1102	180-184	1.0191191843762368
1102	185-189	1.042264887655012
1102	190-194	1.0875723089529181
1102	195-199	1.0163035581377535
1102	200-204	1.0950857823955786
1102	205-209	0.8154251909730164
1102	210-214	1.0075022575857204
1102	215-219	1.169459749469393
1102	220-224	0.9219371704414243
1102	225-229	0.9529126769003007
1102	230-234	1.083668740240082
1102	235-239	1.1073999029146222
1102	240-244	1.0561192220233657
1102	245-249	1.0380119143013964
1102	250-254	1.3355015622399549
1102	255-259	1.1199595215563924
1102	260-264	1.0035546492319867
1102	265-269	0.8625238275729608
1102	270-274	1.0613569962999634
1102	275-279	0.7655290255618965
1102	280-284	1.1991048617807678
1102	285-289	0.7948472959436685
1102	290-294	0.7241752403512649
1102	295-299	0.41043896042303984
1102	300-301	0.3719268823340869
1103	1	0.28108360705380875
1103	2	0.07631196042869703
1103	3	0.006569252774625056
1103	4	0.014502170142364434
1103	5	0.010426626099693692
1103	6	0.0813295454387486
1103	7	0.11585314793646262
1103	8	0.16968041008257018
1103	9	0.12528739122185328
1103	10-14	0.23632981931404373
1103	15-19	0.17807571549349177
1103	20-24	0.2381434829564384
1103	25-29	0.11541205510329888
1103	30-34	0.17110127090671057
1103	35-39	0.18548498257412405
1103	40-44	0.15320663632755327
1103	45-49	0.20329526503584816
1103	50-54	0.37827849609355724
1103	55-59	0.42031415796420646
1103	60-64	0.6121947258588563
1103	65-69	0.6480297201832173
1103	70-74	0.43847403444545563
1103	75-79	0.1259147914812786
1103	80-84	0.2987480098945383
1103	85-89	0.37667316096683834
1103	90-94	0.47154324190917407
1103	95-99	0.32670697844077523
1103	100-104	0.5785526370295173
1103	105-109	0.46496562167662603
1103	110-114	0.44076911529469953
1103	115-119	0.6267212773775981
1103	120-124	0.6766989307614253
1103	125-129	0.529921737899322
1103	130-134	0.6815071656750931
1103	135-139	0.718221613253256
1103	140-144	0.7524905665716659
1103	145-149	0.3632583996670036
1103	150-154	0.7573439154230854
1103	155-159	0.43321866728739167
1103	160-164	0.8218886996817147
1103	165-169	0.6410996655113514
1103	170-174	0.6757010120423885
1103	175-179	0.7979555642710174
1103	180-184	0.8829670726943846
1103	185-189	0.23927797044384747
1103	190-194	-0.013182446627347133
1103	195-199	-0.05674842896221577
1103	200-204	1.0760735025211545
1103	205-209	0.8292233771426787
1103	210-214	-0.13346831328133035
1103	215-219	1.0080611873221628
1103	220-224	0.9656351142197472
1103	225-229	1.2451066592580666
1103	230-234	1.407554449253059
1103	235-239	1.5200897884856914
1103	240-244	1.6926892111995784
1103	245-249	1.4366164870407587
1103	250-254	1.674197628805949
1103	255-259	1.5578440555139466
1103	260-264	1.8530616521476375
1103	265-269	1.7411727531384606
1103	270-274	1.6987857461427147
1103	275-279	1.1797600127419905
1103	280-284	1.2470158828951377
1103	285-289	1.1618645536360006
1103	290-294	-1.2119614751037702
1103	295-299	-1.0384650550941679
1103	300-301	1.1885585887153063
2116	1	0.37265212404020787
2116	2	0.14590404328400552
2116	3	0.05580884440130518
2116	4	0.03813391359189211
2116	5	0.052519603587178665
2116	6	-0.8072849005346612
2116	7	-0.3809798976107217
2116	8	-0.7107757908802839
2116	9	-0.09120531477157101
2116	10-14	-0.17848749214296333
2116	15-19	0.19474856250215566
2116	20-24	0.25355068988156404
2116	25-29	0.16806438208032404
2116	30-34	0.18695762574250097
2116	35-39	0.19750801013238117
2116	40-44	0.19961179195125567
2116	45-49	0.18278254194324717
2116	50-54	0.4091443511806787
2116	55-59	0.3791011631019856
2116	60-64	0.46104857582442804
2116	65-69	0.5065787673537159
2116	70-74	0.08895977240968733
2116	75-79	0.26589657814528067
2116	80-84	0.1195546264413494
2116	85-89	0.3926186310080695
2116	90-94	0.3795288691314411
2116	95-99	-0.1973883669094647
2116	100-104	-0.06312692978197276
2116	105-109	0.26666843282459496
2116	110-114	0.4331369432082113
2116	115-119	0.5790628558637465
2116	120-124	0.5131100695515514
2116	125-129	0.5064633802898229
2116	130-134	0.6000649369104849
2116	135-139	0.506574107209758
2116	140-144	0.6475948262732629
2116	145-149	0.006884719981130161
2116	150-154	0.4965154832658172
2116	155-159	0.596471986987325
2116	160-164	0.6622454846194685
2116	165-169	0.5038805000126878
2116	170-174	0.6829749698905303
2116	175-179	0.10920202483703179
2116	180-184	0.669760346257462
2116	185-189	0.7616516250816012
2116	190-194	0.7676854301660647
2116	195-199	0.9613509058170564
2116	200-204	0.8275279872428811
2116	205-209	0.5434779289187404
2116	210-214	0.43306770185881405
2116	215-219	0.8684121381653718
2116	220-224	0.5587988633656238
2116	225-229	0.92093047390658
2116	230-234	0.4878560519397368
2116	235-239	1.0956654670878052
2116	240-244	0.9228079186820395
2116	245-249	1.0129375231674942
2116	250-254	0.9198968172847231
2116	255-259	0.13669368306236152
2116	260-264	0.7243003579528349
2116	265-269	0.9119971745348536
2116	270-274	0.5587487527807653
2116	275-279	0.35079867005653753
2116	280-284	0.7761731962182807
2116	285-289	0.4724679518516659
2116	290-294	0.46531520189100206
2116	295-299	0.8341762579793013
2116	300-301	0.5103700071867294
1117	1	0.28165874640502864
1117	2	0.050447681915720466
1117	3	-0.009139825546832014
1117	4	0.037113466947083396
1117	5	-0.0141794366168142
1117	6	-0.09956630415442191
1117	7	0.012657972573762777
1117	8	0.23121467869471957
1117	9	0.2550356352905965
1117	10-14	-0.1331284958387542
1117	15-19	0.131962444474496
1117	20-24	0.3029445592996822
1117	25-29	0.13008037987093957
1117	30-34	0.18559348027442013
1117	35-39	0.18368007868911462
1117	40-44	0.11863350828027563
1117	45-49	0.1819013231988862
1117	50-54	0.37873313733919645
1117	55-59	0.47144704779936575
1117	60-64	-0.1729430467109765
1117	65-69	0.4776482611992421
1117	70-74	0.5861480667521164
1117	75-79	0.36869537072427505
1117	80-84	0.6073340531106837
1117	85-89	0.568099779860681
1117	90-94	0.5811729255119076
1117	95-99	0.5441280549392573
1117	100-104	0.6430761787637351
1117	105-109	0.5731162474514164
1117	110-114	0.5027831731241648
1117	115-119	0.36124512309466184
1117	120-124	0.5733472200740266
1117	125-129	0.7545534710643409
1117	130-134	0.6783538099588853
1117	135-139	0.6881064782321289
1117	140-144	0.7159668686794305
1117	145-149	0.7556557082551407
1117	150-154	0.8320415931572782
1117	155-159	0.31360839508723615
1117	160-164	0.12885190378741385
1117	165-169	0.8916922159040972
1117	170-174	1.0444570733161527
1117	175-179	1.0877262781189394
1117	180-184	0.9925899569540633
1117	185-189	1.0342335140282621
1117	190-194	1.080322388080166
1117	195-199	1.2200199396252742
1117	200-204	0.9074702234603578
1117	205-209	0.7618108270859025
1117	210-214	0.6380133537764721
1117	215-219	1.0090517374714167
1117	220-224	1.2175992137342604
1117	225-229	1.2077891740218512
1117	230-234	0.801255928076273
1117	235-239	1.231343062495199
1117	240-244	1.0889237773843838
1117	245-249	1.2278597094237682
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2108	245-249	-1.2699912248354686
2108	250-254	-1.967036350212414
2108	255-259	-0.5314279785501306
2108	260-264	-0.20782220934172457
2108	265-269	-0.6431065020854163
2108	270-274	-1.383530911687373
2108	275-279	-0.7728685892276879
2108	280-284	-1.2324909919446156
2108	285-289	-0.7476526633541631
2108	290-294	-1.0326463424371575
2108	295-299	-1.2160843238585564
2108	300-301	1.4865066755130663
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	7.0
11	94.0
12	614.0
13	1947.0
14	2561.0
15	2710.0
16	7429.0
17	28165.0
18	103144.0
19	293557.0
20	592646.0
21	752230.0
22	649176.0
23	570033.0
24	537033.0
25	550270.0
26	577417.0
27	623796.0
28	702482.0
29	806780.0
30	934710.0
31	1121146.0
32	1380004.0
33	1745785.0
34	2259132.0
35	2911427.0
36	3092295.0
37	859617.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	4.096624277398587	87.78936925995278	4.235791869188055	3.8782145934605867
2	85.13683202292103	6.869860605460754	4.605299284708049	3.388008086910168
3	3.929450706135877	4.930663287818603	4.641023372887417	86.4988626331581
4	3.699452014281865	5.577283497693356	86.16941452341484	4.553849964609936
5	4.005703156422184	5.871234940508259	3.9851878644040593	86.1378740386655
6	4.302364702478281	5.564311010500371	30.917303142151503	59.21602114486985
7	5.452794052479444	59.905074369828746	6.6457796040757104	27.9963519736161
8	7.128121125695394	68.80791513131659	15.915270801617742	8.14869294137028
9	7.917552405318492	23.683487990049564	64.37266061116523	4.026298993466709
10-14	37.73714528621841	21.34275950197968	25.440566369883516	15.4795288419184
15-19	4.8408281033157685	39.24954208968006	44.09535166598148	11.814278141022685
20-24	19.863311299846533	43.41745629615023	29.72793074568064	6.991301658322596
25-29	36.9751343763472	5.06239136193443	49.07896999209759	8.883504269620781
30-34	27.422087729927032	14.238917490006614	38.750789282034425	19.58820549803193
35-39	4.480764355243934	86.0984846779907	4.6431886127147335	4.777562354050636
40-44	36.87366659485525	4.935060098671448	21.315160037992616	36.87611326848069
45-49	20.690774045758197	20.925742839535307	52.68832149708377	5.695161617622721
50-54	31.06804647561734	28.878722737818315	28.606820732877303	11.44641005368705
55-59	37.217071736290656	11.58614430342695	34.44490334051969	16.75188061976271
60-64	30.265143329637578	20.189793457441215	27.182437848733315	22.362625364187892
65-69	33.684608513505054	18.352207954749993	28.196184184112283	19.766999347632666
70-74	32.204826760203765	10.81331382753898	32.04366942861879	24.938189983638463
75-79	19.605154252539588	12.265047076104645	26.549414838482488	41.580383832873274
80-84	30.95376350663101	16.92602654754594	30.91491048107318	21.20529946474987
85-89	33.551291651326	19.353711629509856	28.838617273474267	18.25637944568988
90-94	27.149695131386657	20.227348478363247	29.193737455467367	23.42921893478273
95-99	23.022361583440578	18.513100312398155	28.540302453023465	29.924235651137803
100-104	26.840794783085276	18.038185033211985	27.168103244095033	27.952916939607707
105-109	20.41074799685461	14.975535347744916	38.870892497289326	25.742824158111144
110-114	23.143297942130328	16.751644992519594	33.14532985640327	26.959727208946806
115-119	28.68714754975051	14.977364619461827	32.76896030266131	23.56652752812635
120-124	25.503838548791546	18.942385361772462	27.551697876002873	28.002078213433112
125-129	25.10017053941709	20.973426886356116	33.97997032683127	19.946432247395524
130-134	23.60088207613939	22.110295791116442	28.931140117978195	25.35768201476597
135-139	26.552770811632765	20.5072928883058	29.906065162181495	23.03387113787994
140-144	28.866177906143186	17.69560813617402	33.66107865130508	19.777135306377716
145-149	35.42030278127962	19.650026344384454	26.12982007405613	18.799850800279792
150-154	27.598629620070213	26.575685903025338	23.930917718927514	21.89476675797694
155-159	19.090336747400265	26.712731790829675	31.782627346667606	22.41430411510245
160-164	16.354226946136965	28.19504933634539	39.00167252240131	16.44905119511634
165-169	13.889658515114395	38.22426817137425	34.58293899827089	13.30313431524047
170-174	14.580670885571449	47.31218750065739	25.790581275712466	12.316560338058698
175-179	23.15643458256532	28.45755427413356	32.149397467243425	16.236613676057694
180-184	24.27552750482619	37.011765023603246	22.604340287607364	16.1083671839632
185-189	24.623128601280996	32.578128564561936	22.691679532156538	20.107063302000526
190-194	27.4194099364006	23.223167411299148	31.093798372916492	18.26362427938376
195-199	23.23503866725522	26.472172073988137	30.201411958263684	20.091377300492955
200-204	19.49803632149038	25.062543220703386	29.409689598192273	26.029730859613963
205-209	17.216972270781135	31.63044586630535	36.7106057498975	14.441976113016011
210-214	25.203176271652588	31.984464587399437	30.341694590208846	12.470664550739126
215-219	20.281222141485564	39.033292030470434	28.466138610881643	12.219347217162362
220-224	21.39153242170314	42.70327977394855	26.13737005089015	9.767817753458147
225-229	25.58142357816431	36.79605759151979	23.954858278972623	13.667660551343278
230-234	26.71670667180348	27.93122148119637	30.400152159984113	14.95191968701604
235-239	17.95505333189881	19.33157079321867	45.50188775763442	17.2114881172481
240-244	23.4042191494915	15.72012391577863	45.854828748381806	15.020828186348067
245-249	22.913775435658835	17.83437930847808	47.31031571766442	11.941529538198665
250-254	25.759495600848226	20.738576918602117	41.40264968143113	12.099277799118527
255-259	25.76729563300395	18.94809955047156	41.04212777905934	14.24247703746515
260-264	25.43856886985213	31.509374716522952	31.15175478575586	11.900301627869057
265-269	22.55521897418111	28.613909240302466	32.96498984099709	15.86588194451933
270-274	26.548294906182377	36.70137348991565	26.134876202189105	10.615455401712858
275-279	26.645278454458705	34.68740998796496	26.595975418679473	12.071336138896857
280-284	33.04338291697934	27.418657291250597	23.8107450707011	15.727214721068966
285-289	38.8736356087098	23.931549614764982	22.407571384095686	14.787243392429536
290-294	33.99305396735764	21.497629043392383	26.880730903107903	17.62858608614207
295-299	25.83172575717043	24.10347387023015	33.55549863917275	16.509301733426668
300-301	26.689790355732107	18.395210830293486	38.44624984817854	16.468748965795864
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5.5
1	1.0
2	0.25
3	0.25
4	0.0
5	0.0
6	0.25
7	0.25
8	0.0
9	0.25
10	0.5
11	2.25
12	2.25
13	4.25
14	7.25
15	10.75
16	17.25
17	21.25
18	40.75
19	56.75
20	101.75
21	164.0
22	265.25
23	426.75
24	683.75
25	1071.5
26	1326.0
27	2170.5
28	4017.25
29	7726.75
30	11462.25
31	10738.75
32	16337.25
33	26326.5
34	42026.25
35	62395.0
36	90511.0
37	220671.0
38	511932.0
39	716930.75
40	387934.0
41	443154.5
42	521318.25
43	561910.5
44	528205.0
45	421456.0
46	392402.25
47	416250.0
48	416863.0
49	556163.25
50	224699.5
51	89579.0
52	73141.75
53	60193.5
54	29023.75
55	19598.25
56	13950.5
57	10623.25
58	9326.0
59	9101.25
60	9123.25
61	7538.75
62	6811.5
63	13129.5
64	23169.5
65	13747.5
66	5398.0
67	2225.0
68	1160.0
69	922.5
70	800.0
71	479.5
72	228.5
73	139.25
74	97.0
75	51.75
76	40.5
77	34.25
78	20.75
79	17.5
80	12.25
81	11.5
82	12.5
83	10.75
84	9.75
85	11.75
86	10.75
87	10.25
88	6.5
89	4.5
90	5.75
91	5.25
92	3.75
93	4.75
94	5.0
95	4.5
96	3.75
97	5.0
98	28.5
99	23.25
100	68.66666666666677
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	9.475885458718377E-7
80-84	0.0
85-89	9.475885458718377E-7
90-94	9.475885458718377E-7
95-99	1.8951770917436753E-6
100-104	1.8951770917436753E-6
105-109	2.842765637615513E-6
110-114	7.580708366974701E-6
115-119	5.685531275231026E-6
120-124	6.633119821102863E-6
125-129	6.633119821102863E-6
130-134	8.528296912846538E-6
135-139	6.633119821102863E-6
140-144	7.580708366974701E-6
145-149	5.685531275231026E-6
150-154	8.528296912846538E-6
155-159	4.737942729359188E-6
160-164	1.1371062550462052E-5
165-169	9.475885458718376E-6
170-174	1.2318651096333888E-5
175-179	8.528296912846538E-6
180-184	9.475885458718376E-6
185-189	9.475885458718376E-6
190-194	3.7903541834873507E-6
195-199	1.1371062550462052E-5
200-204	1.2318651096333888E-5
205-209	5.685531275231026E-6
210-214	1.0423474004590214E-5
215-219	1.2318651096333888E-5
220-224	1.1371062550462052E-5
225-229	1.1371062550462052E-5
230-234	3.7903541834873507E-6
235-239	8.528296912846538E-6
240-244	2.842765637615513E-6
245-249	5.685531275231026E-6
250-254	8.528296912846538E-6
255-259	2.842765637615513E-6
260-264	3.7903541834873507E-6
265-269	8.528296912846538E-6
270-274	8.528296912846538E-6
275-279	7.580708366974701E-6
280-284	2.842765637615513E-6
285-289	0.0
290-294	1.8951770917436753E-6
295-299	5.685531275231026E-6
300-301	7.106914094038782E-6
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
301	2.1106207E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	3.1379059061951438
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.8710650309609	2.255246394451875
2	11.679497335578318	0.732983273414033
3	5.72921555870497	0.5393321801857632
4	2.720479650810019	0.3414643665584185
5	1.6225653205314396	0.2545728651241511
6	1.1957750239486398	0.22513377060774462
7	0.6361244201688728	0.13972690025860074
8	0.45329604938869295	0.11379202805053643
9	0.3769965957942502	0.1064681860102417
>10	1.9509003091689792	1.1677130533888394
>50	0.28456798587713567	0.6442902531074794
>100	1.3487653403200794	9.294841087757124
>500	0.04802232843512306	1.037371921514916
>1k	0.05807897365649315	4.1802369288407215
>5k	0.008420835107471856	1.8444571741347813
>10k+	0.016229241548739087	77.12236961659475
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG	3933483	18.636617180907965	No Hit
AGCTCTCTAAGTGTAAATCGTTATTGTTGCGGTTAAAAAGCTCGTAGTTG	2291477	10.856886791643804	No Hit
AGCTCTCATGGCGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTG	1441382	6.829185367129205	No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGTGGTTAAAAAGCTCGTAGTTG	580498	2.75036627850755	No Hit
AGCTCCAAGAGCATATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG	560161	2.654010737220572	No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGCGGTTAAAAAGCTCGTAGTTG	445956	2.1129139878141063	No Hit
AGCTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTG	430900	2.041579522080874	No Hit
AGCTCCAAAAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG	354366	1.678965813232098	No Hit
AGCTCCAATAGCGTATATTTAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG	342962	1.6249343143464858	No Hit
AGCTCCAATAGGATATATCAAAGTTGTTGCAGTTAAAAAGCTCGTAGTCG	260821	1.2357549606141927	No Hit
AGCTCCAATAGCGTATACTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG	258528	1.224890857935772	No Hit
AGCTCCAATAAGGAAAACTAAAGTTGCTGTGTTTAAAAAGCTCGTAGTTG	231997	1.0991884993831436	No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTCG	231010	1.094512149909266	No Hit
AGCTCTCAAAGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTG	224208	1.0622846634641647	No Hit
AGCTCCTCTAGTGTACATCGATATTGCTGCGGTTAAAAAGCTCGTAGTTT	218693	1.0361549093117488	No Hit
AGCTCTCCTAGTGTATCTCGTTATTGCTGCGGTTAAAAAGCTCGTAGTTG	209773	0.993892460165865	No Hit
AGCTCTAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG	184906	0.8760740383148901	No Hit
AGCTCTCAATGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTG	181956	0.8620971072632804	No Hit
AGCTCCAATAGGATATATCAAAGTTGTTGCAGTTGAAAAGCTCGTAGTCG	161718	0.7662106223065092	No Hit
AGCTCCACTAGTGTACATCGATATTGCTGCGGTTAAAAAGCTCGTAGTTT	153775	0.7285771432072091	No Hit
AGCTCCAGTAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG	150836	0.7146523295256225	No Hit
AGCTCCAATAGCGTACGTTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTG	147107	0.696984541087842	No Hit
AGCTCTCAAGGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTG	134729	0.638338285983834	No Hit
AGCTCATGTAGTGTATATTAAAATTGTTGCAGTTAAAAAGCTCGTAGTTG	133791	0.6338940957036951	No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAACGCTCGTAGTCG	127880	0.6058881162304529	No Hit
AACCCCAAGAGTGTATCTTAATGTTGCTGCAGTTAAAAAGCTCGTAGTTG	124978	0.5921386064298526	No Hit
AGCTCTCAAGGTGTATATCCCTATTGCTGCGGTTAAAAAGCTCGTAGTTG	119933	0.5682356853602355	No Hit
AGCTCCAGTAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTG	110126	0.52177068101341	No Hit
AGCTCCAATAGTGTATATTAAAGTTGCTGCGGTTAAAAAGCTCGTAGTTG	94901	0.4496355029589163	No Hit
AGCTCTCAAGGTGTATATCGCCATTGCTGCGGTTAAAAAGCTCGTAGTTG	88132	0.41756436862388396	No Hit
AGCTCCGCAAGTGTATTCCTATACTGTTGCGGTTAAAAAGCTCGTAGTTG	77159	0.3655749230546256	No Hit
AGCTCCAATAGCGTATATTAAAATTGTTGCAGTTAAAAAGCTCGTAGTTG	74921	0.35497140722631976	No Hit
AGCTCCAGTAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTCG	74432	0.35265455323166306	No Hit
AGCTCTTCTAGCGTATATCGAAGTTGATGCAGTTAAAACGCTCGTAGTCG	65380	0.3097666956455037	No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGTTCGTAGTTG	63770	0.3021386078512354	No Hit
AACTCCAGTAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTG	56570	0.26802542019984926	No Hit
AGCTCCAATAGCGTATATTAAAGTTGCTGCGGTTAAAAAGCTCGTAGTTG	55450	0.262718924342967	No Hit
AGCTCCAATAGCGTATACTTAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG	53929	0.2555125134516117	No Hit
AGCTCCAATAGCTTTGTTGAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTG	53257	0.2523286159374823	No Hit
AGCTCCAATAGCATATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG	51391	0.24348761480449801	No Hit
AGCTCCAATAGCGTATGTTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG	51137	0.2422841773512408	No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGTGGTTAAAAAGCTCGTAGTTC	50253	0.23809583597848727	No Hit
AGCTCCAATAGCGTATATTAAAATTGTTGTGGTTAAAAAGCTCGTAGTTG	49891	0.23638070071045925	No Hit
AACTCCAAGAGTGTATCTTAATGTTGCTGCAGTTAAAAAGCTCGTAGTTG	44715	0.2118571091432961	No Hit
AGCTCCAAGAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG	43958	0.20827048649717117	No Hit
AGCTCTCAAAGTGTATGTCATTATTGCTGCGGTTAAAAAGCTCGTAGTTG	43639	0.20675908276650562	No Hit
AGCTCCAGTAGCGTATATTAAAGTTGTTGCAGTTAAAACGCTCGTAGTCG	42879	0.20315824629219262	No Hit
AGCTCCAATAGTGTATATTAACGTTGCTGCAGTTAAAAAGCTCGTAGTTG	41765	0.19788017809168645	No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAACGCTCGTAGTTG	40148	0.19021892469831267	No Hit
AGCTCTCATACTGCATTGAATTGCTGTTGCGGTTAAAAAGTTCGTAGTTG	39884	0.18896810781776185	No Hit
AGCTCCAATAGCGTATGCTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTCG	39321	0.18630064606113264	No Hit
AGCTCTGATAGTATATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG	39260	0.18601163155464173	No Hit
AGCTCCAATAGTGTATATTAAAATTGTTGCAGTTAAAAAGCTCGTAGTTG	38824	0.1839458885246411	No Hit
AGCTCCAATAGTGTATATTAAAGTTGCTGTGATTAAAACGCTCGTAGTTG	34853	0.16513151794635578	No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGAGGTTAAAAAGCTCGTAGTTG	32503	0.1539973525323617	No Hit
AGCTCCAATAGCGTATGCTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG	32044	0.15182263681958583	No Hit
AAGAACGAAAGTGCCTGACTGACTGTATGCGCTGTAATCTCGTATGCCGT	30800	0.14592863606426298	No Hit
AGCTCCACTAGCGTATGTTAAAGCTGCTGCAGTTAAAACGCTCGTAGTTG	28473	0.13490344333304416	No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTCT	27245	0.12908524966139107	No Hit
ATCACTGATAGCTTTCTCTTATGTTGTTGCAGTTAAAAAGCTCGTAGCCG	25605	0.12131502358524202	No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAACGCTCGTAGTCT	25409	0.12038638681028761	No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGCAGCTGAAAAGCTCGTAGTTG	24555	0.11634018371941485	No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGCGGTTAAAACGCTCGTAGTTG	23675	0.11217079411757877	No Hit
AGCTCCAAGAGTGTATATTAAAGTTGTTGCGGTTAAAAAGCTCGTAGTTG	23517	0.11142219916634001	No Hit
AGCTCCAATAGCGTATATTAAGATTGTTGCAGTTGAAACGTCCGTAGTTG	23016	0.10904848985893106	No Hit
GATAAACTCGATTTTCTTGCCACATCTTAATCAAGAACGAAAGTGCCTGA	22729	0.10768870029560498	No Hit
AGCTCTCAAAATGCATAGAACCATTGCTGCGGTTAAAAAGCTCGTAGTTG	21997	0.10422052621771405	No Hit
AGCTCCAATAGCGTATATTAACGTTGTTGCAGTTAAAAAGCTCGTAGTTG	21543	0.10206950021858499	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	6.111946120873353E-4	0.0	0.0
2	6.206704975460537E-4	0.0	0.0
3	6.206704975460537E-4	0.0	0.0
4	6.206704975460537E-4	0.0	0.0
5	6.348843257341312E-4	0.0	0.0
6	6.396222684634904E-4	0.0	0.0
7	6.443602111928495E-4	0.0	0.0
8	6.490981539222087E-4	0.0	0.0
9	6.490981539222087E-4	0.0	0.0
10-14	6.841589301194667E-4	0.0	0.0
15-19	7.315383574130587E-4	0.0	0.0
20-24	9.314795405920163E-4	0.0	0.0
25-29	0.0017729381693262082	0.0	0.0
30-34	0.004365540430831556	0.0	0.0
35-39	0.0052960723828777	0.0	0.0
40-44	0.00547611420659335	0.0	0.0
45-49	0.005773657009997107	0.0	0.0
50-54	0.006186805615997228	0.0	0.0
55-59	0.006747778035153355	0.0	3.7903541834873502E-6
60-64	0.007451836324736132	0.0	4.737942729359188E-6
65-69	0.008296137719107937	0.0	4.737942729359188E-6
70-74	0.009460724041984428	0.0	9.475885458718376E-6
75-79	0.011013821668668369	0.0	1.1371062550462052E-5
80-84	0.01277823154108173	0.0	1.5161416733949401E-5
85-89	0.015019278452068626	0.0	1.8951770917436752E-5
90-94	0.017784341828922648	0.0	7.959743785323436E-5
95-99	0.020818520352804272	0.0	1.2413409950921074E-4
100-104	0.024662887083406318	0.0	1.2792445369269807E-4
105-109	0.029338288968737962	0.0	1.3266239642205726E-4
110-114	0.035188700650950686	0.0	1.3266239642205726E-4
115-119	0.04227950573970965	0.0	1.3266239642205726E-4
120-124	0.05029420966069366	0.0	1.364527506055446E-4
125-129	0.059804208307063414	0.0	1.3740033915141646E-4
130-134	0.07037929647899313	0.0	1.3740033915141646E-4
135-139	0.0822004635887443	0.0	1.3740033915141646E-4
140-144	0.09461671630530298	0.0	1.45928636064263E-4
145-149	0.10781378198366007	6.633119821102863E-6	1.6961834971105893E-4
150-154	0.1217764992070816	9.475885458718376E-5	1.7719905807803363E-4
155-159	0.13653519080903545	9.475885458718376E-5	1.8288458935326464E-4
160-164	0.1523040118008887	9.475885458718376E-5	1.914128862661112E-4
165-169	0.16816380129314568	9.85492087706711E-5	1.9425565190372672E-4
170-174	0.18396484029555854	9.949679731654296E-5	1.9709841754134223E-4
175-179	0.2000842690493844	9.949679731654296E-5	2.0562671445418878E-4
180-184	0.21667370172196265	9.949679731654296E-5	2.0846948009180426E-4
185-189	0.23334652218657764	9.949679731654296E-5	2.0846948009180426E-4
190-194	0.25020033206345416	9.949679731654296E-5	2.1320742282116342E-4
195-199	0.26732704744154173	1.0233956295415845E-4	2.4637302192667774E-4
200-204	0.284677393716455	1.0423474004590213E-4	2.6721996993585826E-4
205-209	0.3019576184389739	1.1371062550462052E-4	2.729055012110892E-4
210-214	0.3200537168994884	1.3455757351380095E-4	2.776434439404485E-4
215-219	0.33791670857771844	1.9709841754134223E-4	2.795386210321921E-4
220-224	0.3558365555687007	2.245784853716255E-4	2.823813866698076E-4
225-229	0.37400088040451795	2.2742125100924104E-4	2.8711932939916676E-4
230-234	0.3920363331980966	2.2931642810098473E-4	2.9470003776614146E-4
235-239	0.4099580753661707	2.321591937386002E-4	3.1459939722945006E-4
240-244	0.42815651338963934	2.368971364679594E-4	3.3165599105514315E-4
245-249	0.4463710604183878	2.368971364679594E-4	3.344987566927587E-4
250-254	0.4644662112903564	2.368971364679594E-4	3.6197882452304196E-4
255-259	0.4824637605421002	2.368971364679594E-4	3.667167672524011E-4
260-264	0.5004660477365734	2.416350791973186E-4	3.7429747561937586E-4
265-269	0.5180627670334135	2.463730219266778E-4	3.8472094962396607E-4
270-274	0.5358338426226938	2.463730219266778E-4	3.923016579909408E-4
275-279	0.5544615382574424	2.482681990184215E-4	4.036727205414028E-4
280-284	0.5735658709307646	2.51110964656037E-4	4.14096194545993E-4
285-289	0.5932283332576052	2.51110964656037E-4	4.273624341881987E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCTCCA	1094685	0.0	294.3463	1
CCAATAG	885285	0.0	294.02347	5
TAGCGTA	885690	0.0	293.97897	9
CTCCAAT	914025	0.0	293.47	3
TCCAATA	912600	0.0	293.411	4
ATAGCGT	822170	0.0	293.09995	8
AGCTCTC	512355	0.0	292.86948	1
AATAGCG	820185	0.0	292.65652	7
GCTCCAA	1030800	0.0	292.17664	2
CTAAGTG	241955	0.0	291.11658	7
CTCTAAG	243090	0.0	289.95755	5
GCTCTCA	250910	0.0	287.7515	2
TAAGTGT	245110	0.0	287.58	8
CAATAGC	835990	0.0	284.66943	6
TCTCATG	157620	0.0	283.62054	4
CTCATGG	157605	0.0	283.46973	5
CTCTCTA	249655	0.0	283.27213	3
AAGTGTA	287760	0.0	282.81067	9
TCTCTAA	251350	0.0	280.45227	4
TAGTGTA	121930	0.0	276.98724	9
>>END_MODULE