FastQCFastQC Report
Tue 22 Sep 2015
000000000-AHV8J l01n01.33100000034817.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AHV8J l01n01.33100000034817.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21106207
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG393348318.636617180907965No Hit
AGCTCTCTAAGTGTAAATCGTTATTGTTGCGGTTAAAAAGCTCGTAGTTG229147710.856886791643804No Hit
AGCTCTCATGGCGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTG14413826.829185367129205No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGTGGTTAAAAAGCTCGTAGTTG5804982.75036627850755No Hit
AGCTCCAAGAGCATATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG5601612.654010737220572No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGCGGTTAAAAAGCTCGTAGTTG4459562.1129139878141063No Hit
AGCTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTG4309002.041579522080874No Hit
AGCTCCAAAAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG3543661.678965813232098No Hit
AGCTCCAATAGCGTATATTTAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG3429621.6249343143464858No Hit
AGCTCCAATAGGATATATCAAAGTTGTTGCAGTTAAAAAGCTCGTAGTCG2608211.2357549606141927No Hit
AGCTCCAATAGCGTATACTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG2585281.224890857935772No Hit
AGCTCCAATAAGGAAAACTAAAGTTGCTGTGTTTAAAAAGCTCGTAGTTG2319971.0991884993831436No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTCG2310101.094512149909266No Hit
AGCTCTCAAAGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTG2242081.0622846634641647No Hit
AGCTCCTCTAGTGTACATCGATATTGCTGCGGTTAAAAAGCTCGTAGTTT2186931.0361549093117488No Hit
AGCTCTCCTAGTGTATCTCGTTATTGCTGCGGTTAAAAAGCTCGTAGTTG2097730.993892460165865No Hit
AGCTCTAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG1849060.8760740383148901No Hit
AGCTCTCAATGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTG1819560.8620971072632804No Hit
AGCTCCAATAGGATATATCAAAGTTGTTGCAGTTGAAAAGCTCGTAGTCG1617180.7662106223065092No Hit
AGCTCCACTAGTGTACATCGATATTGCTGCGGTTAAAAAGCTCGTAGTTT1537750.7285771432072091No Hit
AGCTCCAGTAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG1508360.7146523295256225No Hit
AGCTCCAATAGCGTACGTTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTG1471070.696984541087842No Hit
AGCTCTCAAGGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTG1347290.638338285983834No Hit
AGCTCATGTAGTGTATATTAAAATTGTTGCAGTTAAAAAGCTCGTAGTTG1337910.6338940957036951No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAACGCTCGTAGTCG1278800.6058881162304529No Hit
AACCCCAAGAGTGTATCTTAATGTTGCTGCAGTTAAAAAGCTCGTAGTTG1249780.5921386064298526No Hit
AGCTCTCAAGGTGTATATCCCTATTGCTGCGGTTAAAAAGCTCGTAGTTG1199330.5682356853602355No Hit
AGCTCCAGTAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTG1101260.52177068101341No Hit
AGCTCCAATAGTGTATATTAAAGTTGCTGCGGTTAAAAAGCTCGTAGTTG949010.4496355029589163No Hit
AGCTCTCAAGGTGTATATCGCCATTGCTGCGGTTAAAAAGCTCGTAGTTG881320.41756436862388396No Hit
AGCTCCGCAAGTGTATTCCTATACTGTTGCGGTTAAAAAGCTCGTAGTTG771590.3655749230546256No Hit
AGCTCCAATAGCGTATATTAAAATTGTTGCAGTTAAAAAGCTCGTAGTTG749210.35497140722631976No Hit
AGCTCCAGTAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTCG744320.35265455323166306No Hit
AGCTCTTCTAGCGTATATCGAAGTTGATGCAGTTAAAACGCTCGTAGTCG653800.3097666956455037No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGTTCGTAGTTG637700.3021386078512354No Hit
AACTCCAGTAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTG565700.26802542019984926No Hit
AGCTCCAATAGCGTATATTAAAGTTGCTGCGGTTAAAAAGCTCGTAGTTG554500.262718924342967No Hit
AGCTCCAATAGCGTATACTTAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG539290.2555125134516117No Hit
AGCTCCAATAGCTTTGTTGAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTG532570.2523286159374823No Hit
AGCTCCAATAGCATATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG513910.24348761480449801No Hit
AGCTCCAATAGCGTATGTTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG511370.2422841773512408No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGTGGTTAAAAAGCTCGTAGTTC502530.23809583597848727No Hit
AGCTCCAATAGCGTATATTAAAATTGTTGTGGTTAAAAAGCTCGTAGTTG498910.23638070071045925No Hit
AACTCCAAGAGTGTATCTTAATGTTGCTGCAGTTAAAAAGCTCGTAGTTG447150.2118571091432961No Hit
AGCTCCAAGAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG439580.20827048649717117No Hit
AGCTCTCAAAGTGTATGTCATTATTGCTGCGGTTAAAAAGCTCGTAGTTG436390.20675908276650562No Hit
AGCTCCAGTAGCGTATATTAAAGTTGTTGCAGTTAAAACGCTCGTAGTCG428790.20315824629219262No Hit
AGCTCCAATAGTGTATATTAACGTTGCTGCAGTTAAAAAGCTCGTAGTTG417650.19788017809168645No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAACGCTCGTAGTTG401480.19021892469831267No Hit
AGCTCTCATACTGCATTGAATTGCTGTTGCGGTTAAAAAGTTCGTAGTTG398840.18896810781776185No Hit
AGCTCCAATAGCGTATGCTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTCG393210.18630064606113264No Hit
AGCTCTGATAGTATATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG392600.18601163155464173No Hit
AGCTCCAATAGTGTATATTAAAATTGTTGCAGTTAAAAAGCTCGTAGTTG388240.1839458885246411No Hit
AGCTCCAATAGTGTATATTAAAGTTGCTGTGATTAAAACGCTCGTAGTTG348530.16513151794635578No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGAGGTTAAAAAGCTCGTAGTTG325030.1539973525323617No Hit
AGCTCCAATAGCGTATGCTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG320440.15182263681958583No Hit
AAGAACGAAAGTGCCTGACTGACTGTATGCGCTGTAATCTCGTATGCCGT308000.14592863606426298No Hit
AGCTCCACTAGCGTATGTTAAAGCTGCTGCAGTTAAAACGCTCGTAGTTG284730.13490344333304416No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTCT272450.12908524966139107No Hit
ATCACTGATAGCTTTCTCTTATGTTGTTGCAGTTAAAAAGCTCGTAGCCG256050.12131502358524202No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAACGCTCGTAGTCT254090.12038638681028761No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGCAGCTGAAAAGCTCGTAGTTG245550.11634018371941485No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGCGGTTAAAACGCTCGTAGTTG236750.11217079411757877No Hit
AGCTCCAAGAGTGTATATTAAAGTTGTTGCGGTTAAAAAGCTCGTAGTTG235170.11142219916634001No Hit
AGCTCCAATAGCGTATATTAAGATTGTTGCAGTTGAAACGTCCGTAGTTG230160.10904848985893106No Hit
GATAAACTCGATTTTCTTGCCACATCTTAATCAAGAACGAAAGTGCCTGA227290.10768870029560498No Hit
AGCTCTCAAAATGCATAGAACCATTGCTGCGGTTAAAAAGCTCGTAGTTG219970.10422052621771405No Hit
AGCTCCAATAGCGTATATTAACGTTGTTGCAGTTAAAAAGCTCGTAGTTG215430.10206950021858499No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTCCA10946850.0294.34631
CCAATAG8852850.0294.023475
TAGCGTA8856900.0293.978979
CTCCAAT9140250.0293.473
TCCAATA9126000.0293.4114
ATAGCGT8221700.0293.099958
AGCTCTC5123550.0292.869481
AATAGCG8201850.0292.656527
GCTCCAA10308000.0292.176642
CTAAGTG2419550.0291.116587
CTCTAAG2430900.0289.957555
GCTCTCA2509100.0287.75152
TAAGTGT2451100.0287.588
CAATAGC8359900.0284.669436
TCTCATG1576200.0283.620544
CTCATGG1576050.0283.469735
CTCTCTA2496550.0283.272133
AAGTGTA2877600.0282.810679
TCTCTAA2513500.0280.452274
TAGTGTA1219300.0276.987249
Produced by FastQC (version 0.11.2)