##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AHV5L l01n02 kokuya8.34200000033d48.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2824333 Sequences flagged as poor quality 0 Sequence length 76 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.064767150332486 34.0 34.0 34.0 34.0 34.0 2 33.44978407291208 34.0 34.0 34.0 34.0 34.0 3 33.33578264319399 34.0 34.0 34.0 34.0 34.0 4 33.45223243859701 34.0 34.0 34.0 34.0 34.0 5 33.4307894996801 34.0 34.0 34.0 34.0 34.0 6 37.114977235333086 38.0 38.0 38.0 37.0 38.0 7 37.147521910482936 38.0 38.0 38.0 37.0 38.0 8 37.26028092296482 38.0 38.0 38.0 37.0 38.0 9 37.32081592361807 38.0 38.0 38.0 37.0 38.0 10-11 37.150454638316376 38.0 38.0 38.0 37.0 38.0 12-13 37.123924834642374 38.0 38.0 38.0 37.0 38.0 14-15 37.0554761071021 38.0 38.0 38.0 37.0 38.0 16-17 37.18703194701192 38.0 38.0 38.0 37.0 38.0 18-19 36.37474352351511 38.0 38.0 38.0 34.5 38.0 20-21 36.88973113297901 38.0 38.0 38.0 36.0 38.0 22-23 36.89039146587885 38.0 37.5 38.0 36.5 38.0 24-25 37.07993055351476 38.0 38.0 38.0 37.0 38.0 26-27 36.685693754950286 38.0 38.0 38.0 35.0 38.0 28-29 36.98708190571013 38.0 38.0 38.0 36.5 38.0 30-31 36.59982179863351 38.0 38.0 38.0 36.0 38.0 32-33 36.2556045976165 38.0 38.0 38.0 33.0 38.0 34-35 36.50008249735424 38.0 37.5 38.0 34.0 38.0 36-37 36.51260828662909 38.0 38.0 38.0 34.0 38.0 38-39 37.02917502999823 38.0 38.0 38.0 37.0 38.0 40-41 35.89118598975404 38.0 37.5 38.0 33.0 38.0 42-43 36.63861945457565 38.0 37.0 38.0 35.0 38.0 44-45 36.727011828987585 38.0 38.0 38.0 36.0 38.0 46-47 36.45306927334701 38.0 37.5 38.0 34.5 38.0 48-49 36.746519620738766 38.0 38.0 38.0 36.0 38.0 50-51 36.43870552799546 38.0 38.0 38.0 35.0 38.0 52-53 35.565013580197515 38.0 37.5 38.0 29.5 38.0 54-55 36.32655710215474 38.0 37.0 38.0 34.0 38.0 56-57 36.77814708817976 38.0 38.0 38.0 36.5 38.0 58-59 36.668725500852766 38.0 38.0 38.0 35.0 38.0 60-61 36.674371081596966 38.0 38.0 38.0 35.5 38.0 62-63 36.513212854149984 38.0 38.0 38.0 34.5 38.0 64-65 35.97557918984765 38.0 37.5 38.0 31.0 38.0 66-67 36.56562009508086 38.0 37.5 38.0 35.0 38.0 68-69 36.7410138960243 38.0 38.0 38.0 36.0 38.0 70-71 36.727952228012775 38.0 38.0 38.0 36.0 38.0 72-73 36.237521921104914 38.0 37.0 38.0 34.0 38.0 74-75 36.318901312274434 38.0 37.0 38.0 34.0 38.0 76 33.3438967713793 37.0 33.0 38.0 22.0 38.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2557863593456773 1101 2 0.07628648799665427 1101 3 0.11118035338123633 1101 4 0.10879070944694291 1101 5 0.0664710151282506 1101 6 0.16964667601500594 1101 7 0.18431283081842054 1101 8 0.14994547710435313 1101 9 0.12381965856560129 1101 10-11 0.16276951755413194 1101 12-13 0.16895722407962666 1101 14-15 0.1839814503640511 1101 16-17 0.172158406911322 1101 18-19 0.7482261275688913 1101 20-21 0.25634279629222334 1101 22-23 0.21119843309305963 1101 24-25 0.2118769875855051 1101 26-27 0.2542599278774347 1101 28-29 0.1934952507592982 1101 30-31 0.567596592704696 1101 32-33 0.6053116159364151 1101 34-35 0.443075247668844 1101 36-37 0.289821979711391 1101 38-39 0.2422979148882476 1101 40-41 0.7798902517374913 1101 42-43 0.31632046134560454 1101 44-45 0.19632980302259995 1101 46-47 0.2386298540360201 1101 48-49 0.24519779751653203 1101 50-51 0.3578204226431936 1101 52-53 0.9166271413490534 1101 54-55 0.385060871483482 1101 56-57 0.20450137630825083 1101 58-59 0.1430831646728592 1101 60-61 0.21781710695676537 1101 62-63 0.34081535266083307 1101 64-65 0.7531251144657389 1101 66-67 0.3787450071930252 1101 68-69 0.2804428655778821 1101 70-71 0.24997060835753615 1101 72-73 0.2259808957934979 1101 74-75 0.5634663364734251 1101 76 0.720820569927973 1102 1 0.1372030485668887 1102 2 0.06971060655860128 1102 3 0.13517898708396103 1102 4 0.09913629117001221 1102 5 0.08299825657372395 1102 6 0.11437592456103118 1102 7 0.18326396496269837 1102 8 0.11935063110426114 1102 9 0.06083080464155444 1102 10-11 0.08185486157768196 1102 12-13 0.10612865436193175 1102 14-15 0.13790523076662708 1102 16-17 0.14934527673409548 1102 18-19 0.6936173004498087 1102 20-21 0.33439729347830394 1102 22-23 0.23766982741516074 1102 24-25 0.09808514810654145 1102 26-27 0.18881362104156807 1102 28-29 0.18662314234752841 1102 30-31 0.5242381166106114 1102 32-33 0.5270803157207808 1102 34-35 0.41600317969642475 1102 36-37 0.30473716667269457 1102 38-39 0.18806634036484837 1102 40-41 0.8121666226092188 1102 42-43 0.32885979679334554 1102 44-45 0.28648400067795166 1102 46-47 0.2959283307463849 1102 48-49 0.20591883541325018 1102 50-51 0.35316346459719483 1102 52-53 0.7756605471746099 1102 54-55 0.3636285918287001 1102 56-57 0.16265334011042398 1102 58-59 0.2401426870755614 1102 60-61 0.30866777552436986 1102 62-63 0.2734484546671112 1102 64-65 0.7946945345025611 1102 66-67 0.4069182771004236 1102 68-69 0.20681579024189034 1102 70-71 0.2522528465881919 1102 72-73 0.2593974824074081 1102 74-75 0.5221263136520378 1102 76 0.8381290325846322 1103 1 0.19694404863927417 1103 2 0.05522002303488449 1103 3 0.06349336671218708 1103 4 0.0738176647773301 1103 5 0.033073533775436204 1103 6 0.055819841934543035 1103 7 0.11241743457178899 1103 8 0.12347190708933908 1103 9 0.08160755836858158 1103 10-11 0.1168444931035566 1103 12-13 0.0845937511078958 1103 14-15 0.11873139170554481 1103 16-17 0.10003474097376852 1103 18-19 0.6661963057938252 1103 20-21 0.24959285069468962 1103 22-23 0.17016328629939892 1103 24-25 0.059101078394327544 1103 26-27 0.15732292290346095 1103 28-29 0.09936782500221852 1103 30-31 0.4536828250138498 1103 32-33 0.4531926347983273 1103 34-35 0.3443096418838465 1103 36-37 0.13114412170195067 1103 38-39 0.0882678918181341 1103 40-41 0.72028027705543 1103 42-43 0.30658789687424104 1103 44-45 0.21790914081863377 1103 46-47 0.26602008892464823 1103 48-49 0.1821220840166049 1103 50-51 0.20146267925056094 1103 52-53 0.7593149410010511 1103 54-55 0.36536195575761354 1103 56-57 0.17230041433228394 1103 58-59 0.18668793657932525 1103 60-61 0.11655978528278155 1103 62-63 0.1441487088506932 1103 64-65 0.6684354828238597 1103 66-67 0.3458570884191232 1103 68-69 0.1866167715759346 1103 70-71 0.22268584844996298 1103 72-73 0.2558902744010396 1103 74-75 0.4120389622538667 1103 76 0.7669378256348693 2116 1 0.14490460824121953 2116 2 0.06520996241717825 2116 3 0.03567813310376522 2116 4 0.057965368591609945 2116 5 0.09359098114571651 2116 6 0.08275727599900051 2116 7 0.1538167502226031 2116 8 0.07426756674607304 2116 9 8.158838334324514E-4 2116 10-11 0.07415127505161934 2116 12-13 0.05945204737849252 2116 14-15 0.1182785100887358 2116 16-17 0.05132927048725833 2116 18-19 0.6579108893667609 2116 20-21 0.24585940231976622 2116 22-23 0.16083652810114302 2116 24-25 0.08649358305270738 2116 26-27 0.10658702823814536 2116 28-29 0.07996630948856165 2116 30-31 0.46910365606157 2116 32-33 0.5004422777386921 2116 34-35 0.3148017176289173 2116 36-37 0.10725559751111291 2116 38-39 0.06968365358927286 2116 40-41 0.7069097879062056 2116 42-43 0.2384370130086637 2116 44-45 0.10197742223258643 2116 46-47 0.03082009845359579 2116 48-49 0.1043836184272493 2116 50-51 0.05818690931040038 2116 52-53 0.6962097874310942 2116 54-55 0.28532952103291365 2116 56-57 0.0449940804343143 2116 58-59 0.13049302879480962 2116 60-61 0.0787417150755445 2116 62-63 0.059922963072104096 2116 64-65 0.7031669178131281 2116 66-67 0.32615773397410663 2116 68-69 0.24441941403022582 2116 70-71 0.2143321596015042 2116 72-73 0.09490988006331946 2116 74-75 0.40507077759664867 2116 76 0.7990320164919069 1117 1 0.17950698917164232 1117 2 0.056826501183500966 1117 3 0.034039797953077766 1117 4 0.0727522419514699 1117 5 0.04635062285922231 1117 6 0.047708302122266844 1117 7 -0.6475459317282528 1117 8 -0.035963088992367886 1117 9 0.029715366249114084 1117 10-11 0.12261541861594338 1117 12-13 0.036838062125873705 1117 14-15 -0.4679869713862317 1117 16-17 -0.16513927894054348 1117 18-19 0.016389758556059064 1117 20-21 0.09670876842263709 1117 22-23 0.1312745758337286 1117 24-25 0.19951441010453408 1117 26-27 0.15588810495635386 1117 28-29 0.10925852647432066 1117 30-31 0.4410103643676777 1117 32-33 0.4818542208897867 1117 34-35 0.3287773455017131 1117 36-37 0.15277437960000384 1117 38-39 0.07184280692376888 1117 40-41 0.7455673616709007 1117 42-43 0.28719802796297245 1117 44-45 0.10750878388821405 1117 46-47 0.11679018654012197 1117 48-49 0.10553303066742359 1117 50-51 0.08405631120219681 1117 52-53 0.7885924808134135 1117 54-55 0.3353528971053237 1117 56-57 0.16370497694748565 1117 58-59 0.22135228686641994 1117 60-61 0.22167220903784113 1117 62-63 0.02918239325403249 1117 64-65 0.7483927895561706 1117 66-67 0.4177741131162733 1117 68-69 0.23441346182241318 1117 70-71 0.14460737767767284 1117 72-73 0.29424649976263595 1117 74-75 0.4022637275056411 1117 76 0.6790551820968957 2117 1 0.09818869424815801 2117 2 0.06279917268923896 2117 3 0.0655521507420076 2117 4 0.08834968550480937 2117 5 0.10007715350898394 2117 6 -0.015465708384226673 2117 7 0.1771182581842936 2117 8 0.11472940303912083 2117 9 0.09974257971099121 2117 10-11 0.09342725378876082 2117 12-13 0.09290722149702901 2117 14-15 -0.010301784801022507 2117 16-17 0.07008784925506006 2117 18-19 0.5018801715846095 2117 20-21 0.31160012655494285 2117 22-23 0.16895897972432294 2117 24-25 0.15225367821983582 2117 26-27 0.2075324307316393 2117 28-29 0.16855885084787303 2117 30-31 0.48677336265730986 2117 32-33 0.47023396297412035 2117 34-35 0.36388920412571935 2117 36-37 0.30453083230938915 2117 38-39 0.12905564916366785 2117 40-41 0.6642834282272148 2117 42-43 0.2774221128402701 2117 44-45 0.20346836008278757 2117 46-47 0.1621698127026434 2117 48-49 0.22699460614275324 2117 50-51 0.2514265728979268 2117 52-53 0.9028210092615367 2117 54-55 0.3641169244604967 2117 56-57 0.16528465629765776 2117 58-59 0.23097444993194216 2117 60-61 0.2260662685042547 2117 62-63 0.27679335133451133 2117 64-65 0.8201777750422323 2117 66-67 0.3924941737869645 2117 68-69 0.2739886068803301 2117 70-71 0.2505394024286076 2117 72-73 0.2360716546783692 2117 74-75 0.42521509103971766 2117 76 0.6776192932359706 1116 1 0.2682351061485022 1116 2 0.07873911928552246 1116 3 0.051169913011882784 1116 4 0.06083977878866875 1116 5 0.14937181352358664 1116 6 -0.1524482987027156 1116 7 0.1340065485759112 1116 8 0.09162557467816157 1116 9 0.09750337939937737 1116 10-11 0.07645540397015083 1116 12-13 0.035545244983858026 1116 14-15 0.1171215627464548 1116 16-17 0.00960564671649422 1116 18-19 0.5771414543283626 1116 20-21 0.23701806962059635 1116 22-23 0.1736294152616864 1116 24-25 0.13799024292397633 1116 26-27 0.12749546425801128 1116 28-29 0.08571931239256259 1116 30-31 0.41524682755781583 1116 32-33 0.37733534682262615 1116 34-35 0.2805101870615161 1116 36-37 0.24724025795877935 1116 38-39 0.13998435513822471 1116 40-41 0.6645322326589991 1116 42-43 0.11781371368229543 1116 44-45 0.036013726970885784 1116 46-47 0.19486959901062306 1116 48-49 0.17689871235577215 1116 50-51 0.26322526332072016 1116 52-53 0.7919603317295199 1116 54-55 0.3127291828177974 1116 56-57 0.026557951661310142 1116 58-59 0.03521876781076827 1116 60-61 0.0556817821551121 1116 62-63 0.2415788475439271 1116 64-65 0.6019599342368949 1116 66-67 0.28847899184896875 1116 68-69 0.17044340096122568 1116 70-71 0.19851998365230372 1116 72-73 0.15101719482331077 1116 74-75 0.37942971325678343 1116 76 0.4337095059158784 2118 1 0.004155668648849087 2118 2 -3.191521688421517E-4 2118 3 0.0052709527408083545 2118 4 0.02087591924237131 2118 5 0.1017352711338404 2118 6 -0.13924383096367876 2118 7 0.13815932182106394 2118 8 0.05083626807857655 2118 9 0.08577901685956846 2118 10-11 0.05472363267260505 2118 12-13 0.05063604425771473 2118 14-15 0.12535208284963772 2118 16-17 0.07207523706948393 2118 18-19 0.5961835159280398 2118 20-21 0.23824267743672323 2118 22-23 0.1307476897551254 2118 24-25 0.07951532750065127 2118 26-27 0.13543063165985103 2118 28-29 0.10376910690395391 2118 30-31 0.3920859878196836 2118 32-33 0.3890092899839459 2118 34-35 0.33578947425753114 2118 36-37 0.25433492599142227 2118 38-39 0.1330617107732408 2118 40-41 0.7065268612949467 2118 42-43 0.17315333753785467 2118 44-45 0.09404196295960077 2118 46-47 0.1918790291885486 2118 48-49 0.19846183107724613 2118 50-51 0.28056869654896843 2118 52-53 0.8104468981941437 2118 54-55 0.26919275016688715 2118 56-57 0.034442523290330485 2118 58-59 0.0038199994077245947 2118 60-61 0.1727959341171399 2118 62-63 0.28192568857337363 2118 64-65 0.7373171108959582 2118 66-67 0.3598900694401479 2118 68-69 0.16965497711724709 2118 70-71 0.259422682797144 2118 72-73 0.015856070659125976 2118 74-75 0.33596283876885025 2118 76 0.46551183514024785 1119 1 0.1731021605797025 1119 2 0.016837468920591903 1119 3 0.05824927336863084 1119 4 0.05251704191145734 1119 5 0.10474975758189231 1119 6 -0.17497861381805535 1119 7 0.1370376918854035 1119 8 0.041256199388577386 1119 9 0.09317298640658578 1119 10-11 0.039497487023986366 1119 12-13 0.03045497310035472 1119 14-15 0.04750388285754781 1119 16-17 0.05429204497544049 1119 18-19 0.49275849172435215 1119 20-21 0.16284425598245633 1119 22-23 0.14249303420074 1119 24-25 0.10716627094560494 1119 26-27 0.1384289681785944 1119 28-29 0.10605225016276876 1119 30-31 0.39188427751519583 1119 32-33 0.40690663008673766 1119 34-35 0.30019658144441763 1119 36-37 0.17375741685322055 1119 38-39 0.1352993914028744 1119 40-41 0.6384889917535403 1119 42-43 0.19110583408181014 1119 44-45 0.09526243894234909 1119 46-47 0.1927402656040087 1119 48-49 0.05603230894478628 1119 50-51 0.20483022079548618 1119 52-53 0.6752265946571825 1119 54-55 0.31525531645772986 1119 56-57 0.05832472269948141 1119 58-59 0.2030213121695681 1119 60-61 0.2684010295701569 1119 62-63 0.32368139826717623 1119 64-65 0.7831570567709534 1119 66-67 0.3114881145817847 1119 68-69 0.2044220554362397 1119 70-71 0.2434960676905078 1119 72-73 0.05923518550886797 1119 74-75 0.4279155641098953 1119 76 0.4155697012234896 2119 1 0.16203929757477198 2119 2 0.036155205332875084 2119 3 0.01499972014836004 2119 4 0.09499879147684709 2119 5 0.09730422238750691 2119 6 0.03180722278626291 2119 7 0.11806989804674117 2119 8 0.11716643220561451 2119 9 0.10501212127726234 2119 10-11 0.11608237317628323 2119 12-13 0.08436043514630143 2119 14-15 0.18644637921237717 2119 16-17 0.09991722898139699 2119 18-19 0.6883262051898953 2119 20-21 0.23345339839350032 2119 22-23 0.2092544020921565 2119 24-25 0.09471411249658246 2119 26-27 0.1704931477694842 2119 28-29 0.11996672077208359 2119 30-31 0.4282789868535488 2119 32-33 0.4296790316920749 2119 34-35 0.32147111784539817 2119 36-37 0.2610636724454025 2119 38-39 0.157762434479217 2119 40-41 0.7451738262209417 2119 42-43 0.2727039970830063 2119 44-45 0.1747128255574566 2119 46-47 0.1949954308093922 2119 48-49 0.15198264920557136 2119 50-51 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-0.04892043151906478 2111 5 -0.009034710459239648 2111 6 0.012675051904281531 2111 7 -0.04946874350530095 2111 8 -0.04668847684661159 2111 9 -0.05282869762088893 2111 10-11 -0.09131700332211778 2111 12-13 -0.04092410905112587 2111 14-15 -0.06798318229089517 2111 16-17 -0.0667180716821889 2111 18-19 0.4616138259831004 2111 20-21 0.08853837298703127 2111 22-23 -0.07526852100113501 2111 24-25 -0.06659851381976978 2111 26-27 -0.16574358645681997 2111 28-29 -0.08068050988259756 2111 30-31 -12.40924402624653 2111 32-33 -10.801586054673532 2111 34-35 -5.301904464130331 2111 36-37 -1.3248887221443653 2111 38-39 -0.29084041464484756 2111 40-41 -6.087483910681023 2111 42-43 -0.6273982833617922 2111 44-45 -0.14038049512037531 2111 46-47 -0.07161368606033847 2111 48-49 -0.1250746196610848 2111 50-51 -0.11344125421340578 2111 52-53 0.18384978440796118 2111 54-55 0.004645479352937798 2111 56-57 -0.06673216409125615 2111 58-59 0.00688476852352693 2111 60-61 -0.060530673085843034 2111 62-63 -0.1552643766192503 2111 64-65 0.3052916579673379 2111 66-67 -0.08795688450268102 2111 68-69 -0.06785090335863231 2111 70-71 -0.10645554061284912 2111 72-73 0.025085103609740145 2111 74-75 0.08578985599204714 2111 76 -0.1078106894580344 2110 1 0.11732195514542099 2110 2 -0.005273466162165619 2110 3 -0.004365917946202558 2110 4 0.004862505455513144 2110 5 -0.06665724499077896 2110 6 0.015333595754540852 2110 7 -0.10987608805736215 2110 8 -0.18339351630793743 2110 9 -0.037082208587072785 2110 10-11 -0.019349810741914553 2110 12-13 -0.018477846533961895 2110 14-15 0.017281924300114326 2110 16-17 -0.07721856581274267 2110 18-19 0.5709450384022645 2110 20-21 0.06606844079959018 2110 22-23 -0.0728896562990613 2110 24-25 -0.07925747241292669 2110 26-27 -0.06218358426161075 2110 28-29 -0.023418466523949633 2110 30-31 0.09402372883396737 2110 32-33 -0.22674984669714604 2110 34-35 -0.22029475060037385 2110 36-37 -0.10183002261889129 2110 38-39 -0.053001535137717326 2110 40-41 0.35808393308742126 2110 42-43 -0.050742820339053196 2110 44-45 -0.11777596001154933 2110 46-47 -0.24019682797655406 2110 48-49 -0.17595184277725906 2110 50-51 -0.3704598979538716 2110 52-53 0.33062595437043996 2110 54-55 -0.04438056608193364 2110 56-57 -0.09869644090323249 2110 58-59 -0.10560544417254647 2110 60-61 -0.2945253048884169 2110 62-63 -0.14641007663613692 2110 64-65 0.5049721383652823 2110 66-67 0.011649402999616143 2110 68-69 -0.24593802534037934 2110 70-71 -0.10839624410895965 2110 72-73 -0.11939825032808216 2110 74-75 0.023159112483710942 2110 76 -0.4215800278928441 2109 1 -0.18563750809454405 2109 2 0.015951102895989777 2109 3 0.03523031211272354 2109 4 -0.12696737926233936 2109 5 -0.13911003967451308 2109 6 0.009240781778565577 2109 7 -0.10182243143979264 2109 8 -0.1368814437862369 2109 9 -0.06182281456786143 2109 10-11 -0.09077397022440437 2109 12-13 -0.09381948825251385 2109 14-15 -0.08175462533076683 2109 16-17 -0.078143177686826 2109 18-19 0.37492741301191046 2109 20-21 0.04951997473631309 2109 22-23 -0.16805740550800152 2109 24-25 -0.12189156967026804 2109 26-27 -0.1211959701317582 2109 28-29 -0.042992332695583 2109 30-31 0.1901085695543543 2109 32-33 0.06731164881219343 2109 34-35 0.053842713941115505 2109 36-37 -0.025261692771110233 2109 38-39 -0.22530061360918552 2109 40-41 0.14925104156146318 2109 42-43 -0.04029368589937121 2109 44-45 -0.058184606489994906 2109 46-47 -0.21018706320347036 2109 48-49 -0.1149026669588693 2109 50-51 -0.14695482070637667 2109 52-53 0.14089632143432596 2109 54-55 -0.08259050084863873 2109 56-57 -0.03915549887921088 2109 58-59 -0.1796572517836239 2109 60-61 -0.19169918603392944 2109 62-63 -0.0016641278554843097 2109 64-65 0.5146345342458645 2109 66-67 -0.08982132925308406 2109 68-69 -0.2094491172067876 2109 70-71 -0.2722824934434058 2109 72-73 -0.2396875202412474 2109 74-75 -0.0648314700168342 2109 76 -0.6777607966878207 2108 1 -0.1970310118869918 2108 2 -0.06569534365311114 2108 3 -0.06046321191806925 2108 4 -0.05378085501261154 2108 5 -8.273071481355032E-4 2108 6 0.034091023065236925 2108 7 0.019009953739121954 2108 8 -0.006329010101566723 2108 9 -0.08207858936653167 2108 10-11 -0.04906488089319083 2108 12-13 -0.031634743386383946 2108 14-15 -3.851220410666656E-5 2108 16-17 -0.11806318657712467 2108 18-19 0.497776220390989 2108 20-21 -0.002548484165792786 2108 22-23 -0.06934461001091563 2108 24-25 -0.16567883959169905 2108 26-27 -0.17507737481134455 2108 28-29 -0.11320352353447305 2108 30-31 0.26769536667382 2108 32-33 0.08782905626487292 2108 34-35 0.014049633530667904 2108 36-37 -0.04296767745555741 2108 38-39 -0.033206167823244925 2108 40-41 0.41118149886504085 2108 42-43 0.008761993702933069 2108 44-45 -0.11953720300880377 2108 46-47 -0.010239588474071581 2108 48-49 -0.05342472359772188 2108 50-51 -0.040673483995789184 2108 52-53 0.3710758116203863 2108 54-55 -0.1672119578564164 2108 56-57 -0.18593434918938812 2108 58-59 -0.03654426456888871 2108 60-61 -0.1765972805499345 2108 62-63 -0.22440725535070527 2108 64-65 0.31953863732821475 2108 66-67 -0.0017534510024077576 2108 68-69 -0.10624456228951829 2108 70-71 -0.193908165059689 2108 72-73 -0.1137430673503701 2108 74-75 0.08826133517213464 2108 76 -0.6367989855323515 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 82.0 13 564.0 14 1490.0 15 2465.0 16 2874.0 17 3497.0 18 4735.0 19 6950.0 20 8651.0 21 8673.0 22 8391.0 23 8089.0 24 8111.0 25 8501.0 26 9090.0 27 10266.0 28 12274.0 29 14400.0 30 18377.0 31 24012.0 32 37150.0 33 57301.0 34 86179.0 35 150135.0 36 408254.0 37 1923822.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.41501763425205 1.4346396122553537 50.980107515650595 3.1702352378419967 2 1.934651473462938 2.0011096425244475 2.835607557607407 93.2286313264052 3 2.039490385871638 48.74520816065244 3.1044143873969534 46.110887066078966 4 2.2871948881381905 1.268193233588249 93.66448644688852 2.7801254313850383 5 2.549168246095627 1.4814471239758202 50.23253277853568 45.73685185139288 6 49.77019352887921 1.443172600398041 3.3262366725170156 45.46039719820574 7 2.3210435879905096 1.5418153595910964 50.969804198017734 45.167336854400666 8 2.354821474663221 2.1486134956465825 93.5700570718821 1.9265079578080915 9 3.4955509849582187 1.9437863736322878 92.55144488982 2.0092177515894902 10-11 71.67032003662457 23.51064835485051 2.157695285931227 2.6613363225936886 12-13 23.436276812967876 26.07622755532014 23.991894723462142 26.495600908249838 14-15 2.3618850893290557 47.31136519666767 26.079006972619727 24.24774274138354 16-17 25.847554095073065 2.05653865886211 47.752867668224674 24.34303957784015 18-19 23.732329013611356 46.8604799788127 2.8242243389855233 26.582966668590423 20-21 26.444084320085487 1.4483242592144765 45.8678916402563 26.239699780443736 22-23 23.42613282498912 1.5849759925617837 49.982668474291096 25.006222708158 24-25 2.9338962509024253 25.6401068854133 23.597182060330706 47.828814803353566 26-27 26.466939273803764 23.11103187903126 26.199973586684006 24.22205526048097 28-29 26.0469994154372 1.8013456628520788 23.846001870176075 48.30565305153465 30-31 47.252253894990424 3.392606325104016 25.851112457348336 23.50402732255722 32-33 46.91773243452525 25.88660756362653 3.163755831907923 24.0319041699403 34-35 1.8069576073359621 46.80630789641306 26.81241907381318 24.57431542243779 36-37 2.6112182947265783 1.7243009234392686 51.044423586028984 44.62005719580517 38-39 47.78852210415698 2.1444355180497485 48.31597407246242 1.7510683053308516 40-41 23.10359649517249 46.66246862533561 27.99561878857769 2.238316090914209 42-43 23.176852729476305 1.9154788050842448 72.81650216174934 2.0911663036901103 44-45 1.769975424286017 3.0960761354981865 92.86654583577786 2.2674026044379327 46-47 2.424360017037651 92.02744506402043 2.652431565258063 2.895763353683861 48-49 2.9606636327940086 23.83488420097772 49.74000941107157 23.464442755156703 50-51 49.635418344791496 23.395470718219133 24.354440499756933 2.6146704372324368 52-53 45.62324980800776 24.882689116332955 26.586259481442166 2.9078015942171125 54-55 2.4449489490084915 68.85494734508998 1.7704180066585633 26.929685699242974 56-57 22.832612160110017 25.82825750363006 2.8034583740656642 48.53567196219426 58-59 1.4741887730660657 26.775932583020484 45.21164820153997 26.53823044237347 60-61 1.8196154631907782 47.40552902225056 27.040649951687705 23.73420556287095 62-63 1.9423878133350423 23.60444041124046 47.9733622062271 26.4798095691974 64-65 23.897394535276117 49.619379159610425 23.9261092796069 2.557117025506553 66-67 23.0844592333836 23.620160937113294 26.270450403688233 27.024929425814875 68-69 2.477700044576897 26.289021160040267 46.99658291001805 24.236695885364792 70-71 44.92938686762503 3.3752039862155065 25.94973751324649 25.745671632912977 72-73 23.946538881923626 71.22651613673034 2.191331546244724 2.6356134351013143 74-75 25.929307910929765 26.62940241111795 23.15208581990863 24.28920385804365 76 43.060963420389875 50.718134157693164 2.329576576133197 3.8913258457837654 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 1.0 2 3.0 3 2.0 4 0.0 5 1.0 6 1.0 7 2.0 8 4.0 9 6.5 10 9.5 11 11.5 12 13.0 13 18.0 14 21.5 15 28.0 16 48.5 17 61.0 18 64.0 19 86.5 20 123.0 21 140.0 22 169.5 23 213.5 24 296.0 25 364.0 26 445.5 27 822.5 28 2366.0 29 3614.0 30 5671.5 31 10536.5 32 70260.0 33 127176.0 34 607977.5 35 612674.5 36 83279.0 37 29988.0 38 21999.0 39 11806.5 40 8552.0 41 7871.5 42 8242.0 43 7291.0 44 6473.0 45 6975.0 46 7344.0 47 9120.0 48 20731.0 49 82908.5 50 135251.0 51 562647.5 52 533228.0 53 46984.5 54 17557.0 55 13460.0 56 7810.5 57 5325.5 58 4393.0 59 4019.5 60 3586.5 61 3587.5 62 3648.0 63 3815.5 64 4158.5 65 4503.0 66 4811.5 67 4951.0 68 5014.5 69 5122.0 70 5038.5 71 4911.0 72 4771.0 73 4415.5 74 4022.5 75 3845.0 76 3586.5 77 3053.0 78 2559.0 79 2340.0 80 2173.5 81 1798.5 82 1426.0 83 1262.0 84 1138.0 85 914.0 86 708.5 87 603.0 88 501.0 89 354.5 90 266.5 91 186.5 92 150.0 93 122.5 94 92.5 95 68.0 96 46.0 97 42.0 98 40.0 99 67.5 100 93.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 2824333.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 6.856837413100257 #Duplication Level Percentage of deduplicated Percentage of total 1 91.58595063396673 6.279899728213363 2 3.688970204625677 0.505893378297789 3 1.8645422148025386 0.3835458845028859 4 0.8945065655462172 0.2453394433960448 5 0.4974697965377691 0.17055347563937742 6 0.2974650007753828 0.1223801487842725 7 0.17438267365028404 0.0836999548617204 8 0.10028674249495091 0.05501199103818647 9 0.07407828188413995 0.045714846124922215 >10 0.5231905248602707 0.7229451781912877 >50 0.09049920326565772 0.4630183047234382 >100 0.14170551070978468 2.1829314757530827 >500 0.026153377836470596 1.2538715788898427 >1k 0.032430188214731016 4.3616152104987576 >5k 0.003138405311103001 1.2367994056265506 >10k+ 0.005230675518505002 81.8867799954585 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TCATTGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTG 1127440 39.9188056082622 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAA 1020374 36.12796366434128 No Hit TCATTGTTTGGCATAGTCACGTTCAGTGCTAGATATTTGTATTTTAATTG 30223 1.0700933636366534 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCATCCAGCACCCTGGAGTTTAACAA 20064 0.7103978178210573 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGTAGTTTAACAA 18875 0.6682993825444804 No Hit GCCTCCCTTAGGTCACTAATTGCCTCATCCGGCACCCTGGAGTTTAACAA 18108 0.641142528165057 No Hit TCATTGTTTGGCATAGTCGCGTTCAGTGCTGGATATTTGTATTTTAATTG 16939 0.5997522246845539 No Hit CATTGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTGG 10479 0.37102565455277403 No Hit CTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAATG 10456 0.3702113029872894 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAA 10180 0.3604390842014734 No Hit ATTGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTGGT 6786 0.24026911840777984 No Hit TCATTGTTTGGCATAGTCACGTTCAGTGCTGGGTATTTGTATTTTAATTG 6512 0.2305677128015712 No Hit TGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTGGTGC 5947 0.2105629895624914 No Hit CCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAAT 5120 0.18128173979484716 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAC 5079 0.17983006961289622 No Hit TTGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTGGTG 5040 0.17844921261055266 No Hit TCATTGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGGATTTTAATTG 4921 0.1742358284239146 No Hit TCATTGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTT 4510 0.15968372001460168 No Hit TCATTGTTTGGCATAGTCACGTTCAGTGCTGGATATGTGTATTTTAATTG 4382 0.1551516765197305 No Hit TCATTGTTTGGCATAGTCACGTCCAGTGCTGGATATTTGTATTTTAATTG 4257 0.15072585279427037 No Hit TTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTGGTGCAAC 3668 0.1298713713999022 No Hit TCATTGTTTGGCATAGTCACGTTCAGCGCTGGATATTTGTATTTTAATTG 3467 0.12275464684936231 No Hit TCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAA 3384 0.11981589989565677 No Hit GTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTGGTGCA 3119 0.11043315359768129 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCATCAGGCACCCTGGAGTTTAACAA 2979 0.10547623102516593 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGCAGTTTAACAA 2964 0.10494513217811073 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 8.851647450920271E-4 0.0 0.0 2 8.851647450920271E-4 0.0 0.0 3 9.205713348957081E-4 0.0 0.0 4 9.205713348957081E-4 0.0 0.0 5 9.205713348957081E-4 0.0 0.0 6 9.205713348957081E-4 0.0 0.0 7 9.205713348957081E-4 0.0 0.0 8 9.205713348957081E-4 0.0 0.0 9 9.205713348957081E-4 0.0 0.0 10 9.559779246993892E-4 0.0 0.0 11 9.559779246993892E-4 0.0 0.0 12 9.913845145030702E-4 0.0 0.0 13 9.913845145030702E-4 0.0 0.0 14 9.913845145030702E-4 0.0 0.0 15 9.913845145030702E-4 0.0 0.0 16 9.913845145030702E-4 0.0 0.0 17 9.913845145030702E-4 0.0 0.0 18 9.913845145030702E-4 0.0 0.0 19 9.913845145030702E-4 0.0 0.0 20 0.0011330108737177946 0.0 0.0 21 0.0011330108737177946 0.0 0.0 22 0.0011330108737177946 0.0 0.0 23 0.0011330108737177946 0.0 0.0 24 0.0011330108737177946 0.0 0.0 25 0.0011330108737177946 0.0 0.0 26 0.0011330108737177946 0.0 0.0 27 0.0012038240533251568 0.0 0.0 28 0.0012038240533251568 0.0 0.0 29 0.0012392306431288377 0.0 0.0 30 0.0012392306431288377 0.0 0.0 31 0.0012392306431288377 0.0 0.0 32 0.0012746372329325189 0.0 0.0 33 0.0013100438227362 0.0 0.0 34 0.0014516701819509243 0.0 0.0 35 0.0015224833615582866 0.0 0.0 36 0.0015578899513619675 0.0 0.0 37 0.0016287031309693298 0.0 0.0 38 0.001734922900380373 0.0 0.0 39 0.0018411426697914162 0.0 0.0 40 0.0020181756188098216 0.0 0.0 41 0.002088988798417184 0.0 0.0 42 0.002159801978024546 0.0 0.0 43 0.0023368349270429514 0.0 0.0 44 0.002584681055668719 0.0 0.0 45 0.0027617140046871246 0.0 0.0 46 0.003009560133312892 0.0 0.0 47 0.0032219996721349784 0.0 0.0 48 0.003469845800760746 0.0 0.0 49 0.0037885051089938757 0.0 0.0 50 0.003965538058012281 0.0 0.0 51 0.004142571007030686 0.0 0.0 52 0.004532043494871178 0.0 0.0 53 0.004956922572515351 0.0 0.0 54 0.005346395060355843 0.0 0.0 55 0.005488021419570568 0.0 0.0 56 0.005735867548196335 0.0 0.0 57 0.005983713676822103 0.0 0.0 58 0.006337779574858914 0.0 0.0 59 0.006585625703484681 0.0 0.0 60 0.007010504781128854 0.0 0.0 61 0.007541603628184071 0.0 0.0 62 0.007931076116024562 0.0 0.0 63 0.008462174963079779 0.0 0.0 64 0.008922460630527632 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTAAGGT 45 5.456968E-12 70.00001 7 AACATTG 65 0.0 70.00001 46 ATAGGAC 50 0.0 70.0 62 TAGGTAT 60 0.0 70.0 30 GCTATAT 35 3.7161954E-9 70.0 28 ATGCTAG 40 1.3460522E-10 70.0 26 GGGAGTA 15 0.002225241 70.0 38 CCTGTAT 25 2.8420673E-6 70.0 36 CCTAAGA 25 2.8420673E-6 70.0 69 TAGCACG 50 0.0 70.0 14 GTACTAG 35 3.7161954E-9 70.0 26 TTACGTA 20 7.930614E-5 70.0 20 TGCTAGG 60 0.0 70.0 27 ATAGTGC 15 0.002225241 70.0 13 ATAGTAC 40 1.3460522E-10 70.0 13 GCGCTAG 40 1.3460522E-10 70.0 26 TAGCTAT 15 0.002225241 70.0 30 TGTAAGC 1160 0.0 70.0 51 TAGTCTG 20 7.930614E-5 70.0 64 TACAACT 20 7.930614E-5 70.0 52 >>END_MODULE