FastQCFastQC Report
Tue 25 Aug 2015
000000000-AHV5L l01n02 kokuya8.34200000033d48.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AHV5L l01n02 kokuya8.34200000033d48.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2824333
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATTGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTG112744039.9188056082622No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAA102037436.12796366434128No Hit
TCATTGTTTGGCATAGTCACGTTCAGTGCTAGATATTTGTATTTTAATTG302231.0700933636366534No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCATCCAGCACCCTGGAGTTTAACAA200640.7103978178210573No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGTAGTTTAACAA188750.6682993825444804No Hit
GCCTCCCTTAGGTCACTAATTGCCTCATCCGGCACCCTGGAGTTTAACAA181080.641142528165057No Hit
TCATTGTTTGGCATAGTCGCGTTCAGTGCTGGATATTTGTATTTTAATTG169390.5997522246845539No Hit
CATTGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTGG104790.37102565455277403No Hit
CTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAATG104560.3702113029872894No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAA101800.3604390842014734No Hit
ATTGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTGGT67860.24026911840777984No Hit
TCATTGTTTGGCATAGTCACGTTCAGTGCTGGGTATTTGTATTTTAATTG65120.2305677128015712No Hit
TGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTGGTGC59470.2105629895624914No Hit
CCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAAT51200.18128173979484716No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAC50790.17983006961289622No Hit
TTGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTGGTG50400.17844921261055266No Hit
TCATTGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGGATTTTAATTG49210.1742358284239146No Hit
TCATTGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTT45100.15968372001460168No Hit
TCATTGTTTGGCATAGTCACGTTCAGTGCTGGATATGTGTATTTTAATTG43820.1551516765197305No Hit
TCATTGTTTGGCATAGTCACGTCCAGTGCTGGATATTTGTATTTTAATTG42570.15072585279427037No Hit
TTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTGGTGCAAC36680.1298713713999022No Hit
TCATTGTTTGGCATAGTCACGTTCAGCGCTGGATATTTGTATTTTAATTG34670.12275464684936231No Hit
TCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAA33840.11981589989565677No Hit
GTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTGGTGCA31190.11043315359768129No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCATCAGGCACCCTGGAGTTTAACAA29790.10547623102516593No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGCAGTTTAACAA29640.10494513217811073No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGGT455.456968E-1270.000017
AACATTG650.070.0000146
ATAGGAC500.070.062
TAGGTAT600.070.030
GCTATAT353.7161954E-970.028
ATGCTAG401.3460522E-1070.026
GGGAGTA150.00222524170.038
CCTGTAT252.8420673E-670.036
CCTAAGA252.8420673E-670.069
TAGCACG500.070.014
GTACTAG353.7161954E-970.026
TTACGTA207.930614E-570.020
TGCTAGG600.070.027
ATAGTGC150.00222524170.013
ATAGTAC401.3460522E-1070.013
GCGCTAG401.3460522E-1070.026
TAGCTAT150.00222524170.030
TGTAAGC11600.070.051
TAGTCTG207.930614E-570.064
TACAACT207.930614E-570.052
Produced by FastQC (version 0.11.2)