##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AHV5L l01n02 kokuya7.34200000033d3b.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2802850 Sequences flagged as poor quality 0 Sequence length 76 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.91367964750165 34.0 34.0 34.0 32.0 34.0 2 33.42192197227822 34.0 34.0 34.0 34.0 34.0 3 33.33011292077707 34.0 34.0 34.0 34.0 34.0 4 33.44576199225788 34.0 34.0 34.0 34.0 34.0 5 33.48689298392708 34.0 34.0 34.0 34.0 34.0 6 37.06638314572667 38.0 38.0 38.0 37.0 38.0 7 37.134924808676885 38.0 38.0 38.0 37.0 38.0 8 37.26093119503363 38.0 38.0 38.0 37.0 38.0 9 37.33418877214264 38.0 38.0 38.0 37.0 38.0 10-11 37.17475373280767 38.0 38.0 38.0 37.0 38.0 12-13 37.130756551367355 38.0 38.0 38.0 37.0 38.0 14-15 37.02465651033769 38.0 38.0 38.0 37.0 38.0 16-17 37.18082416112171 38.0 38.0 38.0 37.0 38.0 18-19 36.473632730970266 38.0 38.0 38.0 35.5 38.0 20-21 36.932854594430665 38.0 38.0 38.0 36.0 38.0 22-23 36.90336229195283 38.0 37.5 38.0 36.5 38.0 24-25 37.08182796082559 38.0 38.0 38.0 37.0 38.0 26-27 36.56774979039193 38.0 38.0 38.0 35.5 38.0 28-29 37.006070963483594 38.0 38.0 38.0 37.0 38.0 30-31 36.618711311700594 38.0 38.0 38.0 36.0 38.0 32-33 36.33472590399058 38.0 38.0 38.0 34.0 38.0 34-35 36.43355192036677 38.0 37.0 38.0 34.0 38.0 36-37 36.60703301996182 38.0 37.5 38.0 35.0 38.0 38-39 37.017850223879265 38.0 38.0 38.0 37.0 38.0 40-41 35.912079312128725 38.0 37.5 38.0 34.0 38.0 42-43 36.16024492926842 38.0 37.0 38.0 34.0 38.0 44-45 36.47445635692242 38.0 37.0 38.0 34.0 38.0 46-47 36.37341884153629 38.0 37.0 38.0 34.0 38.0 48-49 36.437833633622915 38.0 37.5 38.0 35.0 38.0 50-51 36.46861533795958 38.0 38.0 38.0 35.0 38.0 52-53 35.89260556219563 38.0 37.5 38.0 32.5 38.0 54-55 36.44797313448811 38.0 37.0 38.0 34.0 38.0 56-57 36.801440854844174 38.0 38.0 38.0 36.0 38.0 58-59 36.67704479369213 38.0 38.0 38.0 35.0 38.0 60-61 36.62469218830833 38.0 38.0 38.0 35.5 38.0 62-63 36.35366234368589 38.0 37.5 38.0 34.0 38.0 64-65 35.93360044240684 38.0 37.5 38.0 31.0 38.0 66-67 36.51529532440195 38.0 37.5 38.0 35.0 38.0 68-69 36.738554150239935 38.0 38.0 38.0 36.0 38.0 70-71 36.71660452753447 38.0 38.0 38.0 36.0 38.0 72-73 36.35184901082826 38.0 37.0 38.0 34.0 38.0 74-75 36.313385304243894 38.0 37.0 38.0 33.5 38.0 76 34.882367233351765 38.0 36.0 38.0 27.0 38.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2790156047367418 1101 2 0.015156143471578787 1101 3 0.01890892491762486 1101 4 0.062377320249858315 1101 5 0.05079298492093898 1101 6 0.04658708598556416 1101 7 0.11709601356027122 1101 8 0.06923364911204999 1101 9 0.03973409411580775 1101 10-11 0.09584347355677636 1101 12-13 0.0855244221623721 1101 14-15 0.13326365543385776 1101 16-17 0.14065382089275857 1101 18-19 0.6620294098804607 1101 20-21 0.1946348963205935 1101 22-23 0.19942621871582134 1101 24-25 0.15860269212751632 1101 26-27 0.23337274897944127 1101 28-29 0.16200828134508782 1101 30-31 0.5467714366545806 1101 32-33 0.5223633217049226 1101 34-35 0.40803740435168834 1101 36-37 0.24940417570962836 1101 38-39 0.14813544319675742 1101 40-41 0.7402371195972322 1101 42-43 0.37758077326197537 1101 44-45 0.2513743604371754 1101 46-47 0.24106048433390015 1101 48-49 0.2847305855056703 1101 50-51 0.2562004748641229 1101 52-53 0.6714235535545043 1101 54-55 0.3721168421079284 1101 56-57 0.18788729369246226 1101 58-59 0.17226232407531228 1101 60-61 0.17931122681228828 1101 62-63 0.32840595657761895 1101 64-65 0.7219955661545683 1101 66-67 0.32655868136985333 1101 68-69 0.26073448315661807 1101 70-71 0.17275599502526973 1101 72-73 0.25445056919356546 1101 74-75 0.5324091492245202 1101 76 0.49700475421435186 1102 1 0.22460215974105324 1102 2 0.08169959397783089 1102 3 0.12275216954623858 1102 4 0.11689983709423757 1102 5 0.03270006291288752 1102 6 0.11285115311858362 1102 7 0.16873805118748209 1102 8 0.1379192803897311 1102 9 0.09201484022281647 1102 10-11 0.15754687587574523 1102 12-13 0.13423984950167522 1102 14-15 0.18940132343304583 1102 16-17 0.14499834508232112 1102 18-19 0.7228510574978984 1102 20-21 0.30274283412806113 1102 22-23 0.2514812819214285 1102 24-25 0.15977473134772424 1102 26-27 0.21641845815672411 1102 28-29 0.15071180580117272 1102 30-31 0.4978849629951583 1102 32-33 0.5943808583967396 1102 34-35 0.4742234824958089 1102 36-37 0.3287202658264974 1102 38-39 0.171277045009667 1102 40-41 0.804335039543929 1102 42-43 0.4096951753379656 1102 44-45 0.28931628537493026 1102 46-47 0.3280889989724116 1102 48-49 0.25826752646790396 1102 50-51 0.2619538638973822 1102 52-53 0.6659485010624167 1102 54-55 0.34144819564025397 1102 56-57 0.22815702084513845 1102 58-59 0.26464172081779935 1102 60-61 0.3090652158413647 1102 62-63 0.3784015689420741 1102 64-65 0.839102022070584 1102 66-67 0.4432859123043684 1102 68-69 0.20292996660369766 1102 70-71 0.2597649419567034 1102 72-73 0.25580095236126965 1102 74-75 0.504995637130925 1102 76 0.6483708730798341 1103 1 0.258147079421434 1103 2 0.06379812956060249 1103 3 0.05803118130364737 1103 4 0.07113063223185634 1103 5 0.03137203001791278 1103 6 0.024536843798614427 1103 7 0.17255150196944413 1103 8 0.13129915273145798 1103 9 0.05847345286876049 1103 10-11 0.11655130553476312 1103 12-13 0.05430708063408218 1103 14-15 0.1289366970624215 1103 16-17 0.10471051895267891 1103 18-19 0.6434861405386272 1103 20-21 0.24950595644630624 1103 22-23 0.2069821919826751 1103 24-25 0.07688653203389606 1103 26-27 0.22469259761808047 1103 28-29 0.13251505717357048 1103 30-31 0.47116991807432385 1103 32-33 0.4448268815880496 1103 34-35 0.3822366195828337 1103 36-37 0.21444317528919044 1103 38-39 0.12004415349727537 1103 40-41 0.7295753625836952 1103 42-43 0.42433106496714146 1103 44-45 0.28267319759552834 1103 46-47 0.29690985485020605 1103 48-49 0.262738416262124 1103 50-51 0.27152113930568333 1103 52-53 0.6748007239980041 1103 54-55 0.3333393446901667 1103 56-57 0.139511312323215 1103 58-59 0.225140994437524 1103 60-61 0.1823065893218967 1103 62-63 0.22229826325710889 1103 64-65 0.7138500849854594 1103 66-67 0.4234327766887347 1103 68-69 0.26851119811151136 1103 70-71 0.22694551947257224 1103 72-73 0.31616725216539976 1103 74-75 0.515816675228038 1103 76 0.792196116959559 2116 1 0.1476111644447471 2116 2 0.04725156599883462 2116 3 0.07910182197718996 2116 4 0.08705565550068428 2116 5 0.14710849580105645 2116 6 0.07319778549869937 2116 7 0.18350080495235943 2116 8 0.07772591900314296 2116 9 0.09544703018568867 2116 10-11 0.1129955004192098 2116 12-13 0.11142904043132518 2116 14-15 0.1558515862512735 2116 16-17 0.15791663332345962 2116 18-19 0.6755678134815781 2116 20-21 0.3132327375273576 2116 22-23 0.21303661739203505 2116 24-25 0.10450012312381318 2116 26-27 0.22382052986537815 2116 28-29 0.14163931114823924 2116 30-31 0.540836090057212 2116 32-33 0.4505111436498268 2116 34-35 0.3802399744846099 2116 36-37 0.13898535210734764 2116 38-39 0.13761640012332776 2116 40-41 0.7067084846216503 2116 42-43 0.3017514264006067 2116 44-45 0.19030239939903737 2116 46-47 0.1654804877367937 2116 48-49 0.1778490376919848 2116 50-51 0.1314274438762837 2116 52-53 0.5349082150734574 2116 54-55 0.292989982413161 2116 56-57 0.16542607380606 2116 58-59 0.18379511476829435 2116 60-61 0.12005826712673695 2116 62-63 0.15134214739973118 2116 64-65 0.7439576241984227 2116 66-67 0.42331455661024364 2116 68-69 0.3249306333225235 2116 70-71 0.2065750672998803 2116 72-73 0.11271304552689543 2116 74-75 0.43649312626977377 2116 76 0.6510891975879218 1117 1 0.3493438191682614 1117 2 0.11641252356552911 1117 3 0.09064296415848361 1117 4 0.10220640204055798 1117 5 0.041057394441551764 1117 6 0.059220486144738516 1117 7 -0.6667412207712715 1117 8 0.0188970708598859 1117 9 0.07918948468955023 1117 10-11 0.1167644827117087 1117 12-13 0.03503414977028996 1117 14-15 -0.5476758883606294 1117 16-17 -0.15984490070560042 1117 18-19 0.08397865070288901 1117 20-21 0.11930237885029982 1117 22-23 0.196648445350732 1117 24-25 0.21042469247065299 1117 26-27 0.31714603344306624 1117 28-29 0.18944157332264666 1117 30-31 0.5473823807893439 1117 32-33 0.5199542752757864 1117 34-35 0.40308581204863003 1117 36-37 0.21717739385046286 1117 38-39 0.07895818738776228 1117 40-41 0.7971696883783395 1117 42-43 0.41439017227420294 1117 44-45 0.23532405559061687 1117 46-47 0.17591172270358157 1117 48-49 0.24562230738837343 1117 50-51 0.15539686698354416 1117 52-53 0.8055758310871965 1117 54-55 0.3554529024862916 1117 56-57 0.1718261369276135 1117 58-59 0.1944893362771225 1117 60-61 0.32540019745074744 1117 62-63 0.18689303038416938 1117 64-65 0.821298610887105 1117 66-67 0.4770911501504784 1117 68-69 0.3092546309889386 1117 70-71 0.20816470229472372 1117 72-73 0.3186100191194541 1117 74-75 0.4085430803631951 1117 76 0.5406983500535674 2117 1 0.15523609502226066 2117 2 0.027185471742903644 2117 3 0.03568703765462544 2117 4 0.04986291018334299 2117 5 0.08001562204750456 2117 6 -0.08844222730599682 2117 7 0.1409283224706357 2117 8 0.07104343549801229 2117 9 0.05122220128642141 2117 10-11 0.06143046718974432 2117 12-13 0.04834595819465193 2117 14-15 -0.08416610294571569 2117 16-17 0.0032698496671130783 2117 18-19 0.4529591561548685 2117 20-21 0.20881182158992573 2117 22-23 0.14293877787619635 2117 24-25 0.09676545596857977 2117 26-27 0.15385221843555286 2117 28-29 0.05590836583454717 2117 30-31 0.46032204859589143 2117 32-33 0.4184137186478125 2117 34-35 0.316913036513192 2117 36-37 0.2388138995585578 2117 38-39 0.1451164535693863 2117 40-41 0.6165015644524132 2117 42-43 0.2708709346338267 2117 44-45 0.14798376322179507 2117 46-47 0.15299563901967161 2117 48-49 0.22494136969915246 2117 50-51 0.11609127058626001 2117 52-53 0.7669753869136287 2117 54-55 0.3553787549849403 2117 56-57 0.12822305846248128 2117 58-59 0.19298122906204185 2117 60-61 0.20465548737696082 2117 62-63 0.17972141369987327 2117 64-65 0.7572568191261766 2117 66-67 0.3770599852758423 2117 68-69 0.18882982034963902 2117 70-71 0.20423152516124787 2117 72-73 0.21710866067083145 2117 74-75 0.4066341478026416 2117 76 0.6385623605322337 1116 1 0.3018744089328962 1116 2 0.020445003713327026 1116 3 0.012824035407355439 1116 4 0.021977631551486354 1116 5 0.10429884605266437 1116 6 -0.0559195823598273 1116 7 0.14555740024896835 1116 8 0.11896305967631093 1116 9 0.08293509403258525 1116 10-11 0.03607498092528516 1116 12-13 0.005601352019070305 1116 14-15 0.12741105180580803 1116 16-17 -0.045590195381571164 1116 18-19 0.5017160814801116 1116 20-21 0.22560691981732361 1116 22-23 0.13555986399521203 1116 24-25 0.132835246475139 1116 26-27 0.1619937968864491 1116 28-29 0.09046148345405669 1116 30-31 0.46334998793883386 1116 32-33 0.4302947386815603 1116 34-35 0.3027062870728727 1116 36-37 0.19294642346964253 1116 38-39 0.17406243622630768 1116 40-41 0.6705889824289457 1116 42-43 0.22823132651898703 1116 44-45 0.10433721724456291 1116 46-47 0.20960347755374187 1116 48-49 0.2511244275221429 1116 50-51 0.2707690966833596 1116 52-53 0.7195340430640726 1116 54-55 0.25240590956055087 1116 56-57 -0.06241892402970706 1116 58-59 -0.004467957094156816 1116 60-61 0.10333559864528752 1116 62-63 0.2726644249663437 1116 64-65 0.6492518683458641 1116 66-67 0.24284162512710594 1116 68-69 0.16566389894559563 1116 70-71 0.1833722317655031 1116 72-73 0.11465708862873214 1116 74-75 0.42482375742510925 1116 76 0.5654769522175869 2118 1 0.13959354540755697 2118 2 0.05900204096227668 2118 3 0.10341350876186794 2118 4 0.02951740518135182 2118 5 0.08961713075273536 2118 6 -0.13793722669047526 2118 7 0.20847076728052372 2118 8 0.11598665728512003 2118 9 0.10936011055829198 2118 10-11 0.10411622834801193 2118 12-13 0.12086111104217423 2118 14-15 0.14990654577207607 2118 16-17 0.1063057845590194 2118 18-19 0.6386102856739768 2118 20-21 0.24272050871170592 2118 22-23 0.10426464068881103 2118 24-25 0.11065776166543628 2118 26-27 0.2348282882585906 2118 28-29 0.07173553113369024 2118 30-31 0.4375235832655875 2118 32-33 0.4267996655848165 2118 34-35 0.3469192541541162 2118 36-37 0.24862147763850118 2118 38-39 0.18349124105113646 2118 40-41 0.696476403124386 2118 42-43 0.21440984147086084 2118 44-45 0.16879830247413707 2118 46-47 0.2303584123169955 2118 48-49 0.23429259517440926 2118 50-51 0.2755661868019459 2118 52-53 0.7401450423426041 2118 54-55 0.27428340303695364 2118 56-57 0.03265419637996558 2118 58-59 0.062340612833473585 2118 60-61 0.23318349271382033 2118 62-63 0.37109903805887967 2118 64-65 0.8148982778246605 2118 66-67 0.39844640682663623 2118 68-69 0.18337212531761793 2118 70-71 0.27212438024545804 2118 72-73 0.0788193127070258 2118 74-75 0.375175788413479 2118 76 0.41813030992918954 1119 1 0.1631780888311809 1119 2 0.10860197425216711 1119 3 0.09780900660542358 1119 4 0.06720015984330274 1119 5 0.09420814350522733 1119 6 -0.12378045018484585 1119 7 0.2106776306644349 1119 8 0.1474832131819852 1119 9 0.12832494036995 1119 10-11 0.1041224727241925 1119 12-13 0.07392772030711825 1119 14-15 0.07247236382414002 1119 16-17 0.05306084211587603 1119 18-19 0.5492291188676859 1119 20-21 0.22576617410621935 1119 22-23 0.1890914853007999 1119 24-25 0.1650197299238343 1119 26-27 0.02817521571522974 1119 28-29 0.1080167917995496 1119 30-31 0.3882960824123103 1119 32-33 0.4219884513057508 1119 34-35 0.31161031267284756 1119 36-37 0.17151338011762363 1119 38-39 0.21572160811185626 1119 40-41 0.6307238394395043 1119 42-43 0.14991685766745633 1119 44-45 0.12767658368050405 1119 46-47 0.26161865154254116 1119 48-49 0.0852735246168379 1119 50-51 0.2448155623643231 1119 52-53 0.5827410932594859 1119 54-55 0.28191825829154027 1119 56-57 0.1290319733820482 1119 58-59 0.24984670206988113 1119 60-61 0.27351037223226626 1119 62-63 0.41220279942286453 1119 64-65 0.7754449904127014 1119 66-67 0.3551490465300091 1119 68-69 0.24842910040216992 1119 70-71 0.298979309036298 1119 72-73 0.07859519390098058 1119 74-75 0.4896471690023887 1119 76 0.6843141428126174 2119 1 0.1973394768306278 2119 2 0.09925244913885933 2119 3 0.0659588434114653 2119 4 0.09706956600290795 2119 5 0.11716448718706829 2119 6 -0.020490680357937663 2119 7 0.034275400089157415 2119 8 0.10988042418945554 2119 9 0.09319798165570603 2119 10-11 0.11528457228024536 2119 12-13 0.07502984028175064 2119 14-15 0.1629085596259543 2119 16-17 0.133452720315951 2119 18-19 0.6420195325759792 2119 20-21 0.24653069140787665 2119 22-23 0.2269659709691183 2119 24-25 0.08755873985468554 2119 26-27 0.2230920815403934 2119 28-29 0.1123127954810883 2119 30-31 0.4621167450107322 2119 32-33 0.43927628948849673 2119 34-35 0.3642235913246026 2119 36-37 0.15979242825598305 2119 38-39 0.18646878122120114 2119 40-41 0.6825242806819531 2119 42-43 0.3456321750835727 2119 44-45 0.23032110894849467 2119 46-47 0.19198032725834224 2119 48-49 0.1777237833295402 2119 50-51 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-0.026649075203422967 2111 3 -0.07346968944600007 2111 4 -0.0918963244804516 2111 5 0.004319484184748035 2111 6 0.046180687162653555 2111 7 -0.1454649641145238 2111 8 -0.06944811306752285 2111 9 -0.05600832737241035 2111 10-11 -0.12752959995060564 2111 12-13 -0.07378463047340489 2111 14-15 -0.08912953175503446 2111 16-17 -0.09234205044609922 2111 18-19 0.44988789821964303 2111 20-21 0.02860281978377799 2111 22-23 -0.13743719435288426 2111 24-25 -0.10862065115972541 2111 26-27 -0.23333723017221786 2111 28-29 -0.10682464201915565 2111 30-31 -12.449276477603426 2111 32-33 -10.765787112776628 2111 34-35 -5.823705097926656 2111 36-37 -1.2927584780746812 2111 38-39 -0.3375995908911662 2111 40-41 -6.129204912364241 2111 42-43 -0.8013427350125895 2111 44-45 -0.195185724465901 2111 46-47 -0.17770035016283003 2111 48-49 -0.17226132058846133 2111 50-51 -0.14252267389436923 2111 52-53 0.13606532584508102 2111 54-55 -0.11499519441736794 2111 56-57 -0.11126753140609935 2111 58-59 -0.018028314327864337 2111 60-61 -0.032466933290493216 2111 62-63 -0.24968810638848993 2111 64-65 0.30158037018444617 2111 66-67 -0.15383339148652198 2111 68-69 -0.10118702841850791 2111 70-71 -0.14578030004149412 2111 72-73 -0.028026296981877863 2111 74-75 0.09003423774174024 2111 76 -0.042348096463257434 2110 1 0.022117921102669413 2110 2 -0.03811343264478495 2110 3 0.02037582351985634 2110 4 -0.0482595044107299 2110 5 -0.0746649440863365 2110 6 0.08008017695510006 2110 7 -0.08258541849836831 2110 8 -0.23494614147497117 2110 9 -0.10518236976165696 2110 10-11 -0.05068095913700432 2110 12-13 -0.0059939233167440875 2110 14-15 0.03355622446933637 2110 16-17 -0.07972107108714255 2110 18-19 0.4740981486860889 2110 20-21 0.03390785124689444 2110 22-23 -0.09732612583198375 2110 24-25 -0.06471001371592422 2110 26-27 -0.19882527367352765 2110 28-29 -0.09737509843289871 2110 30-31 0.05650165999939816 2110 32-33 -0.23880043072085755 2110 34-35 -0.12190861818429255 2110 36-37 -0.09670635543185568 2110 38-39 -0.13689512284351935 2110 40-41 0.2346417756275514 2110 42-43 -0.18908254098031563 2110 44-45 -0.2120532141351319 2110 46-47 -0.20985195262684186 2110 48-49 -0.16065924853792524 2110 50-51 -0.3582812300626088 2110 52-53 0.32064185930637734 2110 54-55 -0.038905160856380405 2110 56-57 -0.10850129963548483 2110 58-59 -0.1465631890004957 2110 60-61 -0.37135079305471663 2110 62-63 -0.22298048659686742 2110 64-65 0.4365162280786521 2110 66-67 0.002062096053982998 2110 68-69 -0.29101178649304416 2110 70-71 -0.18835556289192823 2110 72-73 -0.21578724625273793 2110 74-75 -0.02668457336603325 2110 76 -0.5511537381160281 2109 1 -0.18101079283331245 2109 2 -0.03702456093061102 2109 3 0.003775991145232638 2109 4 -0.06147308101784432 2109 5 -0.15835633417141537 2109 6 0.09869408384202671 2109 7 -0.02796201678535226 2109 8 -0.1085451786689049 2109 9 -0.07056358729946055 2109 10-11 -0.03623098053400753 2109 12-13 -0.060984683969714126 2109 14-15 -0.007073763903065355 2109 16-17 -0.04992915210460325 2109 18-19 0.4745474435317476 2109 20-21 0.14651696878907217 2109 22-23 -0.10300819321386712 2109 24-25 -0.09546276899250472 2109 26-27 -0.06451234749818724 2109 28-29 -0.03223804854143708 2109 30-31 0.21991949304661773 2109 32-33 0.1369621725888024 2109 34-35 0.13418066016598118 2109 36-37 -0.014714856408879484 2109 38-39 -0.21395943508999693 2109 40-41 0.19799256866939174 2109 42-43 -0.02887784324281739 2109 44-45 -0.09244776612444383 2109 46-47 -0.17907651534086 2109 48-49 -0.03838517716036449 2109 50-51 -0.04357204419542171 2109 52-53 0.22801769707396602 2109 54-55 -0.054066245392903056 2109 56-57 -0.021545526256737446 2109 58-59 -0.17165723522241905 2109 60-61 -0.28406151044364947 2109 62-63 -0.11828204742007387 2109 64-65 0.5330537174752621 2109 66-67 -0.09806391041262685 2109 68-69 -0.16481555354957322 2109 70-71 -0.22937894844094586 2109 72-73 -0.1437938214147323 2109 74-75 -0.008506367336288179 2109 76 -0.5754277658710976 2108 1 -0.16690125560170088 2108 2 -0.039918808497283464 2108 3 -0.06715775255748468 2108 4 -0.0257357286987272 2108 5 0.017067578121945814 2108 6 -0.033808632633686386 2108 7 -0.002439492702428936 2108 8 0.024558308568735754 2108 9 -0.06867673612443781 2108 10-11 -0.0884851986829105 2108 12-13 -0.022465937919264434 2108 14-15 -0.031848800472310757 2108 16-17 -0.12332154313097021 2108 18-19 0.48312068147608045 2108 20-21 -0.010265881622764539 2108 22-23 -0.10923888526227188 2108 24-25 -0.14704170781463688 2108 26-27 -0.15335191169834417 2108 28-29 -0.143393342319996 2108 30-31 0.2863467963676811 2108 32-33 0.21643412421591535 2108 34-35 0.07684003576221698 2108 36-37 -0.039988888930814426 2108 38-39 -0.011878692500935983 2108 40-41 0.30852351281215107 2108 42-43 -0.0670843129881078 2108 44-45 -0.202519175944893 2108 46-47 -0.05864366201972615 2108 48-49 -0.0385386935586709 2108 50-51 -0.03227434687374142 2108 52-53 0.35253059204546844 2108 54-55 -0.0545485297764472 2108 56-57 -0.1234506113098277 2108 58-59 -0.017455442686745926 2108 60-61 -0.24358495670749392 2108 62-63 -0.20263800587253655 2108 64-65 0.37819415216550567 2108 66-67 -0.04189042335089965 2108 68-69 -0.10483240381796577 2108 70-71 -0.1574514922124024 2108 72-73 -0.06595786136552562 2108 74-75 0.09042926808898955 2108 76 -0.737288210060548 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 51.0 13 371.0 14 916.0 15 1355.0 16 1884.0 17 2932.0 18 5139.0 19 7945.0 20 8926.0 21 8705.0 22 8456.0 23 8166.0 24 8281.0 25 8718.0 26 9168.0 27 10436.0 28 12184.0 29 14605.0 30 18501.0 31 28578.0 32 41998.0 33 53946.0 34 84800.0 35 155615.0 36 390609.0 37 1910565.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.77360543732272 2.1307597623847156 53.55117112938616 3.544463670906399 2 2.5126924380541236 2.0238685623561734 3.7877160747096705 91.67572292488003 3 2.665037372674242 51.122143532475874 3.8659935422873146 42.34682555256257 4 2.8305831564300625 1.5795351160425994 92.1724673100594 3.4174144174679344 5 2.742636958809783 1.580212997484703 53.44884670959916 42.22830333410636 6 52.239078081238745 1.822430740139501 4.5677792247176985 41.37071195390406 7 2.824161121715397 1.9642506734216958 53.98522932015627 41.226358884706634 8 3.3548709349412205 2.627753893358546 91.8998876143925 2.1174875573077405 9 4.306937581390371 2.203578500454894 91.1077296323385 2.381754285816223 10-11 72.1326685338138 22.100397809372602 2.6863906381005047 3.0805430187130955 12-13 21.74420678951781 27.747471323831103 22.41432827300783 28.093993613643253 14-15 2.779064166830191 46.74634746775603 27.877999179406675 22.596589186007098 16-17 27.45330645592879 2.671227500579767 47.145155823536754 22.73031021995469 18-19 22.114437090818274 46.49410421535223 3.4630465419126963 27.9284121519168 20-21 27.928483507858072 1.897657741227679 42.35083932425924 27.82301942665501 22-23 21.6086305010971 2.4946215459264676 52.629662664787624 23.267085288188806 24-25 3.0166080953315375 27.853006760975436 21.96983427582639 47.16055086786663 26-27 4.2948962663003725 45.35651212159053 27.954974401056067 22.393617211053034 28-29 28.433415987298645 2.0316998769110013 22.16675883475748 47.368125301032876 30-31 47.491428367554455 3.5832099470182137 27.30115775014717 21.62420393528016 32-33 71.03050466489465 3.490482901332572 3.542804645271777 21.93620778850099 34-35 3.121429973063132 46.58206825195783 28.108853488413576 22.18764828656546 36-37 28.094992596821093 20.808088195943412 29.44315607328255 21.653763133952943 38-39 47.588490286672496 2.9711365217546426 47.49990188558075 1.9404713059921153 40-41 22.527819897604225 46.12276789696202 28.93884795832813 2.410564247105625 42-43 45.32736321958007 2.6971832242182066 49.318301015038266 2.6571525411634584 44-45 2.6151774087089925 4.207360365342419 90.4970654869151 2.6803967390334837 46-47 3.758799079508358 90.33091317765846 2.665964999910805 3.2443227429223827 48-49 27.43550314858091 22.37731237847191 28.6306973259361 21.556487147011076 50-51 52.51246053124498 22.075548102823912 22.317533938669566 3.0944574272615375 52-53 45.32354567672191 23.709706191911803 27.392154414256918 3.5745937171093707 54-55 2.5215584137574254 66.31924648126014 2.75396471448704 28.40523039049539 56-57 20.83099345309239 27.899405961788894 3.6305010970975973 47.63909948802112 58-59 1.6534777101878444 28.7191430151453 41.95294075672976 27.6744385179371 60-61 2.147367857716253 46.998501525233245 29.394205897568547 21.459924719481958 62-63 2.3861783541752146 22.327309702624117 71.84811887899815 3.438393064202508 64-65 22.097614927662914 52.31919296430419 22.412258950710886 3.1709331573220116 66-67 21.42308721479922 22.30444012344578 27.826854808498492 28.445617853256504 68-69 2.903901386089159 28.317819362434665 46.48550582442871 22.292773427047468 70-71 40.81463510355531 4.597320584405159 27.37340564068716 27.214638671352375 72-73 22.071891110833615 72.35776085056283 2.5303173555488163 3.040030683054748 74-75 26.57807231924648 29.17642756480011 21.279233637190718 22.966266478762687 76 1.9851579642149957 90.40551581426048 2.8086412044882887 4.800685017036231 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.5 3 0.5 4 0.0 5 0.0 6 0.0 7 0.5 8 1.0 9 1.5 10 1.5 11 1.0 12 1.0 13 3.5 14 7.5 15 12.0 16 15.5 17 16.0 18 18.0 19 25.0 20 32.0 21 34.0 22 46.0 23 77.0 24 114.5 25 133.0 26 157.5 27 257.5 28 458.5 29 584.0 30 875.5 31 2153.5 32 5655.5 33 8171.0 34 14685.5 35 61408.0 36 637958.5 37 1174301.0 38 665920.5 39 94970.5 40 24327.0 41 14797.5 42 13342.0 43 12171.0 44 12676.5 45 21348.0 46 28343.0 47 75047.5 48 500225.0 49 486812.5 50 94927.0 51 59047.0 52 16613.5 53 8995.5 54 7931.0 55 6422.0 56 4220.0 57 3314.0 58 3101.0 59 3165.0 60 3457.5 61 3843.0 62 4000.0 63 4262.0 64 4727.5 65 5210.5 66 5646.0 67 5802.0 68 5737.5 69 5643.5 70 5359.0 71 5104.0 72 4879.0 73 4349.0 74 3632.5 75 3221.0 76 2879.5 77 2249.0 78 1704.0 79 1448.0 80 1271.5 81 978.5 82 754.5 83 647.0 84 561.0 85 416.0 86 306.5 87 256.0 88 226.5 89 170.0 90 133.0 91 101.0 92 79.0 93 65.5 94 46.0 95 38.0 96 36.0 97 27.0 98 22.0 99 63.5 100 101.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 2802850.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 6.97493098774397 #Duplication Level Percentage of deduplicated Percentage of total 1 90.16095106920531 6.28866411497068 2 3.8552179976408993 0.5377975895250754 3 2.1548944873668407 0.4509072100576091 4 1.1912522902280667 0.33235610053330866 5 0.7666486328013155 0.2673660652818722 6 0.386011999232972 0.16154442330546354 7 0.24716818746769353 0.12067867349670486 8 0.17893718544915066 0.09984596157191764 9 0.11191351101161935 0.07025301143119529 >10 0.6183345164962465 0.886616247430806 >50 0.11602172322019418 0.5597591505396745 >100 0.14078831535247277 2.2574944372104833 >500 0.029681339607072785 1.4490681002388428 >1k 0.03332641573940921 4.9488487873329765 >5k 0.0036450767214978815 1.733164890226796 >10k+ 0.005207252459282688 79.83563523684657 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TCATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTG 1157896 41.31137948873468 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAA 907850 32.390245642827836 No Hit TCATTGTTTGGCATAGTCACGTTCAATGCTAGGTATGTGTAGTTTAAGTG 30097 1.0737998822626968 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCATCCAGCACACTGGAGTTTAACAA 17506 0.6245785539718501 No Hit GCCTCCCTTAGGTCACTAATTGCCTCATCCGGCACACTGGAGTTTAACAA 17231 0.6147671120466668 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGTAGTTTAACAA 16883 0.6023511782649803 No Hit TCATTGTTTGGCATAGTCGCGTTCAATGCTGGGTATGTGTAGTTTAAGTG 14880 0.5308882030790089 No Hit CTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAATG 13289 0.47412455179549384 No Hit ATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGG 11904 0.4247105624632071 No Hit CATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGG 10568 0.3770447936921348 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAA 8516 0.3038335979449489 No Hit TGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTC 8012 0.28585190074388567 No Hit TTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGT 7140 0.2547407103483954 No Hit TCATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTATTTTAAGTG 7124 0.2541698628182029 No Hit TTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTCAAC 6107 0.2178853666803432 No Hit CCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAAT 5459 0.19476604170754766 No Hit TGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTCAACC 5361 0.19126960058511872 No Hit TCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAATGA 4657 0.16615230925664948 No Hit TCATTGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTG 4605 0.1642970547835239 No Hit TCATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGCTTAAGTG 4595 0.1639402750771536 No Hit TCATTGTTTGGCATAGTCACGTCCAATGCTGGGTATGTGTAGTTTAAGTG 4585 0.16358349537078332 No Hit GTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTCA 4503 0.16065790177854683 No Hit TCATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAATTG 4487 0.16008705424835434 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAC 3990 0.14235510284175037 No Hit TCATTGTTTGGCATAGTCACGTTCAGTGCTGGGTATGTGTAGTTTAAGTG 3947 0.14082095010435808 No Hit GCCTCCCTTAGGTCACTAGTTGAACGAAGCAAAGCCTACAGCAACTGTTA 3841 0.13703908521683286 No Hit CCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAATGAA 3682 0.13136628788554505 No Hit TCATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGCAGTTTAAGTG 3435 0.12255382913819862 No Hit TCATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTATTTTAATTG 3418 0.12194730363736912 No Hit TCATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTCTAAGTG 3134 0.11181475997645254 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGCAGTTTAACAA 2919 0.10414399628949106 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.001748220561214478 0.0 0.0 2 0.001748220561214478 0.0 0.0 3 0.001926610414399629 0.0 0.0 4 0.001926610414399629 0.0 0.0 5 0.001926610414399629 0.0 0.0 6 0.001926610414399629 0.0 0.0 7 0.001926610414399629 0.0 0.0 8 0.001926610414399629 0.0 0.0 9 0.001926610414399629 0.0 0.0 10 0.001962288385036659 0.0 0.0 11 0.001962288385036659 0.0 0.0 12 0.001962288385036659 0.0 0.0 13 0.001962288385036659 0.0 0.0 14 0.001997966355673689 0.0 0.0 15 0.001997966355673689 0.0 0.0 16 0.001997966355673689 0.0 0.0 17 0.001997966355673689 0.0 0.0 18 0.001997966355673689 0.0 0.0 19 0.001997966355673689 0.0 0.0 20 0.001997966355673689 0.0 0.0 21 0.001997966355673689 0.0 0.0 22 0.001997966355673689 0.0 0.0 23 0.001997966355673689 0.0 0.0 24 0.001997966355673689 0.0 0.0 25 0.001997966355673689 0.0 0.0 26 0.001997966355673689 0.0 0.0 27 0.0020336443263107196 0.0 0.0 28 0.00210500026758478 0.0 0.0 29 0.0022120341794958703 0.0 0.0 30 0.0022477121501329003 0.0 0.0 31 0.0023190680914069606 0.0 0.0 32 0.0023190680914069606 0.0 0.0 33 0.002354746062043991 0.0 0.0 34 0.002354746062043991 0.0 0.0 35 0.002497457944592112 0.0 0.0 36 0.0027115257684142925 0.0 0.0 37 0.002747203739051323 0.0 0.0 38 0.0029612715628735036 0.0 0.0 39 0.0031396614160586547 0.0 0.0 40 0.0033180512692438054 0.0 0.0 41 0.0035677970637030165 0.0 0.0 42 0.0037818648875251977 0.0 0.0 43 0.003924576770073318 0.0 0.0 44 0.0041743225645325295 0.0 0.0 45 0.00435271241771768 0.0 0.0 46 0.0044954243002658005 0.0 0.0 47 0.004638136182813921 0.0 0.0 48 0.004959237918547193 0.0 0.0 49 0.005244661683643435 0.0 0.0 50 0.0057084753019248265 0.0 0.0 51 0.006136610949569189 0.0 0.0 52 0.006350678773391369 0.0 0.0 53 0.00656474659721355 0.0 0.0 54 0.0071712720980430635 0.0 0.0 55 0.007563729775050395 0.0 0.0 56 0.008170255275879909 0.0 0.0 57 0.008955170629894572 0.0 0.0 58 0.009490340189450023 0.0 0.0 59 0.009989831778368447 0.0 0.0 60 0.01063203524983499 0.0 0.0 61 0.011238560750664503 0.0 0.0 62 0.011773730310219955 0.0 0.0 63 0.012415933781686497 0.0 0.0 64 0.013058137253153041 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCTTAAT 70 0.0 70.00001 42 CTAAGGT 25 2.84206E-6 70.00001 7 GTGCTAG 70 0.0 70.00001 26 TAGGACT 35 3.7161954E-9 70.00001 63 TTCTAAG 55 0.0 70.0 42 CTGAGTG 155 0.0 70.0 44 ATTACTC 15 0.0022252393 70.0 62 AGTATAC 15 0.0022252393 70.0 64 ACTCGAA 15 0.0022252393 70.0 67 AGCCTAG 15 0.0022252393 70.0 10 TAACTCC 15 0.0022252393 70.0 62 CTAGACT 20 7.930605E-5 69.99999 63 ACTATAC 40 1.3460522E-10 69.99999 64 TTGTAAG 20 7.930605E-5 69.99999 50 AATTACA 20 7.930605E-5 69.99999 49 TGTAAGG 20 7.930605E-5 69.99999 43 TGCTAGG 3920 0.0 69.91071 27 TCATTGT 138330 0.0 69.86844 1 ATGCTAG 3915 0.0 69.8212 26 GCTAGGT 3950 0.0 69.73418 28 >>END_MODULE