FastQCFastQC Report
Tue 25 Aug 2015
000000000-AHV5L l01n02 kokuya7.34200000033d3b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AHV5L l01n02 kokuya7.34200000033d3b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2802850
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTG115789641.31137948873468No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAA90785032.390245642827836No Hit
TCATTGTTTGGCATAGTCACGTTCAATGCTAGGTATGTGTAGTTTAAGTG300971.0737998822626968No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCATCCAGCACACTGGAGTTTAACAA175060.6245785539718501No Hit
GCCTCCCTTAGGTCACTAATTGCCTCATCCGGCACACTGGAGTTTAACAA172310.6147671120466668No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGTAGTTTAACAA168830.6023511782649803No Hit
TCATTGTTTGGCATAGTCGCGTTCAATGCTGGGTATGTGTAGTTTAAGTG148800.5308882030790089No Hit
CTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAATG132890.47412455179549384No Hit
ATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGG119040.4247105624632071No Hit
CATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGG105680.3770447936921348No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAA85160.3038335979449489No Hit
TGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTC80120.28585190074388567No Hit
TTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGT71400.2547407103483954No Hit
TCATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTATTTTAAGTG71240.2541698628182029No Hit
TTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTCAAC61070.2178853666803432No Hit
CCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAAT54590.19476604170754766No Hit
TGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTCAACC53610.19126960058511872No Hit
TCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAATGA46570.16615230925664948No Hit
TCATTGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTG46050.1642970547835239No Hit
TCATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGCTTAAGTG45950.1639402750771536No Hit
TCATTGTTTGGCATAGTCACGTCCAATGCTGGGTATGTGTAGTTTAAGTG45850.16358349537078332No Hit
GTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTCA45030.16065790177854683No Hit
TCATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAATTG44870.16008705424835434No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAC39900.14235510284175037No Hit
TCATTGTTTGGCATAGTCACGTTCAGTGCTGGGTATGTGTAGTTTAAGTG39470.14082095010435808No Hit
GCCTCCCTTAGGTCACTAGTTGAACGAAGCAAAGCCTACAGCAACTGTTA38410.13703908521683286No Hit
CCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAATGAA36820.13136628788554505No Hit
TCATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGCAGTTTAAGTG34350.12255382913819862No Hit
TCATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTATTTTAATTG34180.12194730363736912No Hit
TCATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTCTAAGTG31340.11181475997645254No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGCAGTTTAACAA29190.10414399628949106No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTAAT700.070.0000142
CTAAGGT252.84206E-670.000017
GTGCTAG700.070.0000126
TAGGACT353.7161954E-970.0000163
TTCTAAG550.070.042
CTGAGTG1550.070.044
ATTACTC150.002225239370.062
AGTATAC150.002225239370.064
ACTCGAA150.002225239370.067
AGCCTAG150.002225239370.010
TAACTCC150.002225239370.062
CTAGACT207.930605E-569.9999963
ACTATAC401.3460522E-1069.9999964
TTGTAAG207.930605E-569.9999950
AATTACA207.930605E-569.9999949
TGTAAGG207.930605E-569.9999943
TGCTAGG39200.069.9107127
TCATTGT1383300.069.868441
ATGCTAG39150.069.821226
GCTAGGT39500.069.7341828
Produced by FastQC (version 0.11.2)