##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AHV5L l01n02 kokuya6.34200000033d2e.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2099455 Sequences flagged as poor quality 0 Sequence length 76 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.33236911484171 34.0 34.0 34.0 31.0 34.0 2 33.02858837174409 34.0 34.0 34.0 32.0 34.0 3 33.0201056940968 34.0 34.0 34.0 33.0 34.0 4 33.11340228773658 34.0 34.0 34.0 33.0 34.0 5 33.19599610375074 34.0 34.0 34.0 34.0 34.0 6 36.68333972388072 38.0 38.0 38.0 35.0 38.0 7 36.6201218887759 38.0 38.0 38.0 34.0 38.0 8 36.63935878597064 38.0 38.0 38.0 35.0 38.0 9 36.78525283942737 38.0 38.0 38.0 36.0 38.0 10-11 36.68796187582015 38.0 38.0 38.0 35.0 38.0 12-13 36.6797399801377 38.0 38.0 38.0 35.5 38.0 14-15 36.4745660183238 38.0 38.0 38.0 34.5 38.0 16-17 36.70333086443863 38.0 38.0 38.0 35.5 38.0 18-19 35.95782691222246 38.0 37.5 38.0 31.0 38.0 20-21 36.39852152106142 38.0 37.0 38.0 34.0 38.0 22-23 36.51406626957949 38.0 37.5 38.0 34.0 38.0 24-25 36.644443915206566 38.0 38.0 38.0 35.0 38.0 26-27 35.97649270882205 38.0 37.5 38.0 30.5 38.0 28-29 36.312280329895145 38.0 37.0 38.0 34.0 38.0 30-31 35.77329021103096 38.0 37.0 38.0 31.5 38.0 32-33 35.673508124727604 38.0 37.0 38.0 29.0 38.0 34-35 35.87031086639152 38.0 37.0 38.0 32.5 38.0 36-37 35.70211412009307 38.0 37.0 38.0 30.5 38.0 38-39 36.16813339652434 38.0 37.0 38.0 34.0 38.0 40-41 34.71019169260594 38.0 36.0 38.0 25.5 38.0 42-43 35.54978887377915 38.0 37.0 38.0 31.0 38.0 44-45 35.74826966998579 38.0 37.0 38.0 32.5 38.0 46-47 34.70806876070218 37.5 35.5 38.0 25.5 38.0 48-49 35.67269791445875 38.0 37.0 38.0 31.0 38.0 50-51 35.7164385519099 38.0 37.0 38.0 32.5 38.0 52-53 35.27552460043202 38.0 36.5 38.0 28.5 38.0 54-55 35.16894455942138 38.0 36.0 38.0 27.5 38.0 56-57 35.410425800981685 38.0 36.5 38.0 29.0 38.0 58-59 35.63167774493857 38.0 36.5 38.0 32.0 38.0 60-61 35.75026042472928 38.0 37.0 38.0 31.5 38.0 62-63 35.559343019974236 38.0 37.0 38.0 30.5 38.0 64-65 35.06438670988423 38.0 36.5 38.0 27.5 38.0 66-67 35.69004170129867 38.0 37.0 38.0 31.5 38.0 68-69 35.91238202295358 38.0 37.0 38.0 31.5 38.0 70-71 35.85428694589787 38.0 37.0 38.0 31.5 38.0 72-73 35.01030315010324 38.0 36.0 38.0 28.0 38.0 74-75 34.994862238057024 38.0 36.0 38.0 27.0 38.0 76 32.53351988968566 37.0 31.0 38.0 19.0 38.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.25984700856086107 1101 2 0.027666987467107163 1101 3 -0.017417201445617536 1101 4 0.11477550389833624 1101 5 0.04213138756725954 1101 6 0.16333899780778438 1101 7 0.18232689896483834 1101 8 0.09567181039646755 1101 9 0.02488891153678452 1101 10-11 0.11053272790565671 1101 12-13 0.09258135039713977 1101 14-15 0.1807039784867399 1101 16-17 0.19344166975440658 1101 18-19 0.7236519491157125 1101 20-21 0.2629656849230102 1101 22-23 0.20836083622257462 1101 24-25 0.17794545231964065 1101 26-27 0.2591116134911644 1101 28-29 0.15019878431622402 1101 30-31 0.48891337283407665 1101 32-33 0.3530375946961257 1101 34-35 0.30674219374691347 1101 36-37 0.20958622854410436 1101 38-39 0.1742339896387861 1101 40-41 1.086435335285806 1101 42-43 0.4119453233267336 1101 44-45 0.28201219039176806 1101 46-47 0.3343358878880949 1101 48-49 0.28843289961707796 1101 50-51 0.27165835164569785 1101 52-53 0.673628110029739 1101 54-55 0.5649557032066426 1101 56-57 0.15233915126453468 1101 58-59 0.15598396091950661 1101 60-61 0.22308774351095195 1101 62-63 0.3205705189888448 1101 64-65 0.8144642511125468 1101 66-67 0.29198721209629497 1101 68-69 0.33433027558757544 1101 70-71 0.2607914576011865 1101 72-73 0.30063308037471614 1101 74-75 0.5977368689002702 1101 76 0.444397330497722 1102 1 0.12152359104213417 1102 2 0.06611642260106265 1102 3 0.11252612978458387 1102 4 0.008851484246264363 1102 5 -0.03557663768801689 1102 6 0.08569287474772125 1102 7 0.22076199921200867 1102 8 0.06156727604364676 1102 9 0.022509933222643497 1102 10-11 0.16076995155474805 1102 12-13 0.20120655984481317 1102 14-15 0.14866155675572656 1102 16-17 0.3132918446502373 1102 18-19 0.867338027352865 1102 20-21 0.4092741962024604 1102 22-23 0.19487421039955422 1102 24-25 0.15120215473045562 1102 26-27 0.23244257810647184 1102 28-29 0.235035249135656 1102 30-31 0.5961832544878405 1102 32-33 0.5579664633534946 1102 34-35 0.4640585871711096 1102 36-37 0.3239792039728826 1102 38-39 0.21387940038095365 1102 40-41 1.0335336954224275 1102 42-43 0.3075000102388401 1102 44-45 0.2854911925065906 1102 46-47 0.5963264248198286 1102 48-49 0.3102762536945747 1102 50-51 0.3717854928981339 1102 52-53 0.5980486962891476 1102 54-55 0.5911553987350047 1102 56-57 0.48799122347286783 1102 58-59 0.48545304914919996 1102 60-61 0.39142945248142524 1102 62-63 0.3789784578602635 1102 64-65 0.8625816991213426 1102 66-67 0.5216595254371796 1102 68-69 0.31214388869068443 1102 70-71 0.45872241588326546 1102 72-73 0.4862518180125761 1102 74-75 0.7356104399657255 1102 76 1.0672329073032572 1103 1 0.3126637142630244 1103 2 0.1237752034480124 1103 3 0.06974939820476322 1103 4 0.06075243676098552 1103 5 -0.0037415321484743913 1103 6 0.11299492873227734 1103 7 0.26890531339641655 1103 8 0.18737189564391343 1103 9 0.24681441205633803 1103 10-11 0.21286276228612877 1103 12-13 0.0806112404011472 1103 14-15 0.183682282439527 1103 16-17 0.18477039889526026 1103 18-19 0.7742883536488918 1103 20-21 0.3391331117994909 1103 22-23 0.23148290589483622 1103 24-25 0.21818804844138384 1103 26-27 0.18037599846503127 1103 28-29 0.0874610975570036 1103 30-31 0.43009311086152024 1103 32-33 0.3852594925940167 1103 34-35 0.3459745129193621 1103 36-37 0.21125165013513225 1103 38-39 0.20814573865472852 1103 40-41 0.8572235236094841 1103 42-43 0.28363537404358397 1103 44-45 0.2587422640778385 1103 46-47 0.6178589054669388 1103 48-49 0.4355538747373444 1103 50-51 0.32351241115240725 1103 52-53 0.6987919729589365 1103 54-55 0.5657708339549359 1103 56-57 0.3734943028154589 1103 58-59 0.36152912236336476 1103 60-61 0.19921061038417776 1103 62-63 0.16080200922923638 1103 64-65 0.7020281717718504 1103 66-67 0.4933986314330596 1103 68-69 0.3487966669898839 1103 70-71 0.26313957529077214 1103 72-73 0.44206929431663866 1103 74-75 0.6535140852614347 1103 76 0.9879160592615577 2116 1 0.12616273492661634 2116 2 0.07271627507641654 2116 3 0.04108057818753963 2116 4 0.11195352268267555 2116 5 0.14317841702094114 2116 6 0.16756553280079345 2116 7 0.26181193893857824 2116 8 0.07640143447368075 2116 9 0.16645437928291074 2116 10-11 0.19583992351788737 2116 12-13 0.19990350832842552 2116 14-15 0.29038135401261655 2116 16-17 0.22292929974568665 2116 18-19 0.8171048093527631 2116 20-21 0.4439986382682193 2116 22-23 0.25998345249356447 2116 24-25 0.1635772408193148 2116 26-27 0.12724875908123323 2116 28-29 0.1963009607631605 2116 30-31 0.5600717186943172 2116 32-33 0.6024668142720344 2116 34-35 0.39158957208019984 2116 36-37 0.16266074920828544 2116 38-39 0.2519296756546012 2116 40-41 0.9252202281295681 2116 42-43 0.23208813604502154 2116 44-45 0.2497301607487188 2116 46-47 0.40788922721893783 2116 48-49 0.18862428917592666 2116 50-51 0.1700380366689913 2116 52-53 0.5716767823188107 2116 54-55 0.5290831789449655 2116 56-57 0.2606359879954354 2116 58-59 0.2566337071572278 2116 60-61 0.2104517697127548 2116 62-63 0.1458590596812499 2116 64-65 0.7768472099180102 2116 66-67 0.440003708724781 2116 68-69 0.36689868474445575 2116 70-71 0.3484120738691061 2116 72-73 0.3726342094525279 2116 74-75 0.5963471588956182 2116 76 1.15673948591202 1117 1 0.37018877927378213 1117 2 0.17246715154882253 1117 3 0.04017058501257509 1117 4 0.07072791746096385 1117 5 0.04109303654743712 1117 6 -0.04754699553033248 1117 7 -0.9738355474141329 1117 8 -0.016094923747324685 1117 9 0.06244167654656252 1117 10-11 0.15479404516135986 1117 12-13 0.11860617631712245 1117 14-15 -0.7297835208969374 1117 16-17 -0.3185095878401967 1117 18-19 -0.06717241770416393 1117 20-21 0.10231143304250878 1117 22-23 0.20504658754700955 1117 24-25 0.30499162872255425 1117 26-27 0.30835622810457863 1117 28-29 0.2253097243757125 1117 30-31 0.6151978977914752 1117 32-33 0.5266343912676703 1117 34-35 0.3588250762435834 1117 36-37 0.2982879251052353 1117 38-39 0.16758297898853414 1117 40-41 1.1371155622061693 1117 42-43 0.40039805532841655 1117 44-45 0.34312362488782355 1117 46-47 0.3234011528293834 1117 48-49 0.32723522976922226 1117 50-51 0.19477038467022822 1117 52-53 0.930980885037819 1117 54-55 0.6117031221382163 1117 56-57 0.3456155844457456 1117 58-59 0.3629431726424386 1117 60-61 0.3496903822408015 1117 62-63 0.055329015490812594 1117 64-65 0.7818376698742853 1117 66-67 0.7115258966211186 1117 68-69 0.4378935059429665 1117 70-71 0.4040421759723145 1117 72-73 0.6125867690632063 1117 74-75 0.5440705224988349 1117 76 0.8490564021074718 2117 1 0.22618423192445647 2117 2 0.1126950330389036 2117 3 0.11224758576178573 2117 4 0.22507219222282515 2117 5 0.18630258217865503 2117 6 0.09325011438905761 2117 7 0.2650340088637151 2117 8 0.2380954607705661 2117 9 0.2505395283500107 2117 10-11 0.1912078356424871 2117 12-13 0.23484362146445648 2117 14-15 0.07698881167217309 2117 16-17 0.16734349433036755 2117 18-19 0.6848613557648875 2117 20-21 0.39815076331893096 2117 22-23 0.2819881008045968 2117 24-25 0.24525800917895424 2117 26-27 0.29945273922157156 2117 28-29 0.20107526760722294 2117 30-31 0.5529737278341216 2117 32-33 0.513069147578193 2117 34-35 0.43177758960820256 2117 36-37 0.4080566109226922 2117 38-39 0.3239071427494409 2117 40-41 1.0136493948231546 2117 42-43 0.36987619101377334 2117 44-45 0.3371235248996527 2117 46-47 0.4294700650989185 2117 48-49 0.3962160299280768 2117 50-51 0.23461926170448777 2117 52-53 0.9237287676826895 2117 54-55 0.5439971359450411 2117 56-57 0.34817642583916353 2117 58-59 0.512826056216781 2117 60-61 0.30812646163423096 2117 62-63 0.2328613391313965 2117 64-65 0.928914283345371 2117 66-67 0.5774582546238349 2117 68-69 0.44954339525183684 2117 70-71 0.5115448622742136 2117 72-73 0.5226137165792792 2117 74-75 0.5979950757911254 2117 76 0.8372286465179926 1116 1 0.22559897199973022 1116 2 -0.05129734521550233 1116 3 0.04047234572770719 1116 4 -0.012232162008139369 1116 5 0.0528607300055981 1116 6 -0.08955291534404353 1116 7 0.12302960734238155 1116 8 0.11883018836804382 1116 9 0.09105381947291136 1116 10-11 0.010582769924958768 1116 12-13 0.004785069228709915 1116 14-15 0.20773558493083755 1116 16-17 -0.05830296927520351 1116 18-19 0.5507213976036596 1116 20-21 0.27027306185852495 1116 22-23 0.1727175954939213 1116 24-25 0.17763230260075602 1116 26-27 0.09163576119016881 1116 28-29 0.048657588810506525 1116 30-31 0.3167223236004517 1116 32-33 0.22399656551505132 1116 34-35 0.23762875422330865 1116 36-37 0.10441056336043886 1116 38-39 0.27624518196773096 1116 40-41 0.9719186406317917 1116 42-43 0.1153678333193966 1116 44-45 0.23683045754944487 1116 46-47 0.42105234361314814 1116 48-49 0.2700190699835261 1116 50-51 0.3443904536266089 1116 52-53 0.746427167245578 1116 54-55 0.29837490712506565 1116 56-57 -0.34113551234708694 1116 58-59 0.07641813711743595 1116 60-61 0.02060099035320917 1116 62-63 0.18838114746453982 1116 64-65 0.6234427013957671 1116 66-67 0.33937115110935423 1116 68-69 0.23911048801008405 1116 70-71 0.26772764560399764 1116 72-73 0.33467781804206 1116 74-75 0.5478215115473333 1116 76 0.5039436241298603 2118 1 0.0032847593992499924 2118 2 0.06420624633173588 2118 3 0.1438153299767393 2118 4 0.0502762104924912 2118 5 0.11939480867286534 2118 6 -0.09680407208477959 2118 7 0.25409066647989675 2118 8 0.11971152773176641 2118 9 0.13063596181677894 2118 10-11 0.10641299919860359 2118 12-13 0.1729304439097774 2118 14-15 0.2385994181022184 2118 16-17 0.19314606814876356 2118 18-19 0.7130208213488558 2118 20-21 0.3234958308273974 2118 22-23 0.156490774501016 2118 24-25 0.2076392074336013 2118 26-27 0.23289753667531699 2118 28-29 0.035980227079896565 2118 30-31 0.40588239914715984 2118 32-33 0.4480773392438806 2118 34-35 0.4323438720622548 2118 36-37 0.30031994894781633 2118 38-39 0.23917600091623115 2118 40-41 0.9345213279884703 2118 42-43 0.11255611612351402 2118 44-45 0.1803860688711012 2118 46-47 0.6456828721821068 2118 48-49 0.3368756627864329 2118 50-51 0.3736438634654533 2118 52-53 0.7959617756028905 2118 54-55 0.5192638147655302 2118 56-57 0.13018816406943046 2118 58-59 0.1395359263093141 2118 60-61 0.26547705432431457 2118 62-63 0.3074945129182751 2118 64-65 0.8544429442691523 2118 66-67 0.488380446573494 2118 68-69 0.34814837031362345 2118 70-71 0.37841104745712784 2118 72-73 0.4799384664627908 2118 74-75 0.5525079301977556 2118 76 0.6934135496265839 1119 1 -0.025135727559103316 1119 2 -0.04884876972555929 1119 3 0.10608364546489213 1119 4 0.09223985485508024 1119 5 0.044365010673693916 1119 6 -0.12488363081748588 1119 7 0.23549583592762957 1119 8 0.09152723050559075 1119 9 0.12006943559872951 1119 10-11 0.0765014878930117 1119 12-13 0.021377404425273028 1119 14-15 -0.0157138043727727 1119 16-17 0.03580290484319448 1119 18-19 0.469945572431385 1119 20-21 0.21561031020519295 1119 22-23 0.10677426691714942 1119 24-25 0.1496046780757112 1119 26-27 0.13913898663767554 1119 28-29 0.1425522404501791 1119 30-31 0.3212694208280311 1119 32-33 0.4103090950692092 1119 34-35 0.23338514781934805 1119 36-37 0.1336565759444639 1119 38-39 0.23637163967895702 1119 40-41 0.8798327744066299 1119 42-43 -0.09419962914402902 1119 44-45 -0.010502970133124734 1119 46-47 0.49337921818521124 1119 48-49 0.07034522657752262 1119 50-51 0.24713591366387533 1119 52-53 0.5020133981193666 1119 54-55 0.3086966650229428 1119 56-57 0.11950517845949093 1119 58-59 0.32497876808748316 1119 60-61 0.3033412463186309 1119 62-63 0.4105225729535391 1119 64-65 0.8059409296034445 1119 66-67 0.41281898317026133 1119 68-69 0.2983649275409377 1119 70-71 0.32027496537322264 1119 72-73 0.2600964573590616 1119 74-75 0.5629145547405159 1119 76 0.6399175974593021 2119 1 0.1069434900737889 2119 2 0.1225222698440902 2119 3 -0.004726013756645386 2119 4 0.024730991645007805 2119 5 0.14107074733085057 2119 6 -0.06470177876165195 2119 7 0.20390820331156334 2119 8 0.21047523925078337 2119 9 0.13731322845659122 2119 10-11 0.14471482686664672 2119 12-13 0.15109929257767618 2119 14-15 0.2741637438533431 2119 16-17 0.18302543076524813 2119 18-19 0.7610682616020412 2119 20-21 0.4590573249152783 2119 22-23 0.285050827681701 2119 24-25 0.15386211835271268 2119 26-27 0.14870306213733642 2119 28-29 0.12504695351854167 2119 30-31 0.48346436001938287 2119 32-33 0.42207838900625916 2119 34-35 0.42558264490128295 2119 36-37 0.2399260304734483 2119 38-39 0.28473031520253045 2119 40-41 0.8353822559654631 2119 42-43 0.21304735852258005 2119 44-45 0.2566761942643083 2119 46-47 0.3738950231220386 2119 48-49 0.2878399379428984 2119 50-51 0.3017683693103592 2119 52-53 0.642097484940507 2119 54-55 0.4470797546073513 2119 56-57 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0.026902958889515105 2111 9 -0.14395697259939766 2111 10-11 -0.15916734286154366 2111 12-13 -0.05532443042374524 2111 14-15 -0.10514137630114107 2111 16-17 -0.04667104368181185 2111 18-19 0.4855302761180198 2111 20-21 0.08815503337476827 2111 22-23 -0.021818443365702933 2111 24-25 -0.19185406202939248 2111 26-27 -0.2853191844592331 2111 28-29 -0.12244266373655677 2111 30-31 -11.593855817648581 2111 32-33 -9.709971561664045 2111 34-35 -4.466918350204665 2111 36-37 -1.2719649788760918 2111 38-39 -0.3754153472299677 2111 40-41 -8.196675963609628 2111 42-43 -0.9576684773218247 2111 44-45 -0.20334438071641614 2111 46-47 -0.4242204772642424 2111 48-49 -0.2335389911042256 2111 50-51 -0.19844005573079926 2111 52-53 0.05202957453327883 2111 54-55 -0.11508433126927997 2111 56-57 -0.11849640776824089 2111 58-59 -0.15523830351849455 2111 60-61 -0.17381665329303786 2111 62-63 -0.21260467923562487 2111 64-65 0.2518719866665151 2111 66-67 -0.11549780509748331 2111 68-69 -0.0802992569070966 2111 70-71 -0.15484038265932298 2111 72-73 -0.20583484907015048 2111 74-75 -0.06488021627821894 2111 76 -0.0892715512921356 2110 1 0.08419227791824113 2110 2 -0.07883428295874495 2110 3 -0.10098425270268763 2110 4 0.027287295179434068 2110 5 -0.06853018879314021 2110 6 0.12198866868425995 2110 7 -0.314152621305837 2110 8 -0.26959172070348103 2110 9 -0.2025122836571498 2110 10-11 -0.1386734978907569 2110 12-13 -0.06096961568139392 2110 14-15 -0.01171498589359743 2110 16-17 -0.17789079017460097 2110 18-19 0.47909224619792923 2110 20-21 -0.021139039073901245 2110 22-23 -0.16711234740396463 2110 24-25 -0.22695712363169207 2110 26-27 -0.20389615359183466 2110 28-29 -0.1134601969945166 2110 30-31 -0.17248181154865705 2110 32-33 -0.5248279608264497 2110 34-35 -0.33885849701891857 2110 36-37 -0.2289964078508504 2110 38-39 -0.36398492364062207 2110 40-41 0.1994577325903606 2110 42-43 -0.18787053267188725 2110 44-45 -0.3406156490325998 2110 46-47 -0.7823303056218336 2110 48-49 -0.3456321437348322 2110 50-51 -0.5444011312542045 2110 52-53 0.14051751523300027 2110 54-55 -0.2552038871858642 2110 56-57 -0.23408850349965604 2110 58-59 -0.42504031911874307 2110 60-61 -0.4484273280368072 2110 62-63 -0.3019593608594491 2110 64-65 0.308431696208082 2110 66-67 -0.23945045638513562 2110 68-69 -0.6218463232843305 2110 70-71 -0.3955151357089832 2110 72-73 -0.5968460050219093 2110 74-75 -0.4181470997320176 2110 76 -0.9171541246528285 2109 1 -0.18919751451032596 2109 2 0.00776026002214536 2109 3 -0.04355424409028075 2109 4 -0.1643772703934161 2109 5 -0.2301694164227328 2109 6 -0.0522342802529252 2109 7 -0.20926464497122765 2109 8 -0.19771652567620634 2109 9 -0.06181505335646165 2109 10-11 -0.1303266426665246 2109 12-13 -0.17207992934765315 2109 14-15 -0.07736029213322126 2109 16-17 -0.19219646187973183 2109 18-19 0.3779254838378776 2109 20-21 0.0603118702212484 2109 22-23 -0.20176700871180486 2109 24-25 -0.28674983304418333 2109 26-27 -0.26532369081864005 2109 28-29 -0.14426501994095986 2109 30-31 0.1977140270374136 2109 32-33 -0.07116007510931155 2109 34-35 -0.020227338837756292 2109 36-37 -0.22506387792586935 2109 38-39 -0.41349152024309177 2109 40-41 0.13389090532523795 2109 42-43 0.11038252223531231 2109 44-45 -0.08409942900438949 2109 46-47 -0.4591219977720655 2109 48-49 -0.21336705018414648 2109 50-51 -0.1966016941809201 2109 52-53 -0.02487952908968083 2109 54-55 -0.42518525818078245 2109 56-57 -0.09576633092704867 2109 58-59 -0.3204808311199443 2109 60-61 -0.2822708943211083 2109 62-63 -0.04994895732461657 2109 64-65 0.3742213538337964 2109 66-67 -0.27199395526802306 2109 68-69 -0.363865807288974 2109 70-71 -0.5367743878027937 2109 72-73 -0.5790990848925972 2109 74-75 -0.35486358936592666 2109 76 -0.7804123594843873 2108 1 -0.1289146010589377 2108 2 -0.1397735951843586 2108 3 -0.13068360115862987 2108 4 -0.10710791140596143 2108 5 0.110426497118425 2108 6 -0.1660288801346823 2108 7 -0.020664633208966166 2108 8 -0.025962059884278688 2108 9 0.008391002972409467 2108 10-11 -0.14172087358456764 2108 12-13 -0.11399538341755289 2108 14-15 -0.06455279399815339 2108 16-17 -0.2459927641947246 2108 18-19 0.5128154292671567 2108 20-21 0.03602421471945405 2108 22-23 -0.1280431681462204 2108 24-25 -0.2582051348855927 2108 26-27 -0.25048732560647125 2108 28-29 -0.20943961220819318 2108 30-31 0.19627475902179725 2108 32-33 -0.058193755429492455 2108 34-35 2.2829420430525715E-4 2108 36-37 -0.19846335852896146 2108 38-39 -0.19787658587963364 2108 40-41 0.38213300371349845 2108 42-43 -0.035806627383806244 2108 44-45 -0.30799589630064617 2108 46-47 -0.25244731419250144 2108 48-49 -0.08177397506350559 2108 50-51 -0.07072210883175245 2108 52-53 0.2641835445142462 2108 54-55 -0.1717898015108048 2108 56-57 -0.36322561955757493 2108 58-59 -0.187449385630309 2108 60-61 -0.35104789723381913 2108 62-63 -0.295882091937564 2108 64-65 0.12450111004536524 2108 66-67 -0.056463275873348096 2108 68-69 -0.1200587951212242 2108 70-71 -0.2879101274481286 2108 72-73 -0.5043981095531151 2108 74-75 -0.14415098176441177 2108 76 -0.9280700899242014 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 21.0 13 199.0 14 747.0 15 1416.0 16 2161.0 17 3299.0 18 5551.0 19 8068.0 20 9544.0 21 9582.0 22 9990.0 23 9999.0 24 10393.0 25 11606.0 26 13079.0 27 15245.0 28 17856.0 29 21408.0 30 27013.0 31 37774.0 32 57597.0 33 80501.0 34 118063.0 35 174770.0 36 364879.0 37 1088694.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.84049431876368 4.338887949491654 42.120931384573616 8.69968634717105 2 6.208087336951733 6.5452700819974705 5.302566618479558 81.94407596257123 3 6.7021203121762545 40.597583658616166 7.276983788649911 45.423312240557664 4 6.953423626607858 3.6075552941120437 79.3542609867799 10.084760092500195 5 6.770709541285715 4.237623573736993 43.749544524650446 45.242122360326846 6 44.018900143132385 3.975174509575104 5.978932627753393 46.026992719539116 7 7.089077879735455 4.597907552198071 43.06712932642043 45.24588524164604 8 7.648032465568446 4.972528584799388 80.22629682465211 7.153142124980054 9 9.218249498084026 4.21785653895892 79.30843957122205 7.255454391734998 10-11 63.797890404890786 23.444274823704248 4.827538575487448 7.930296195917512 12-13 25.980456832844716 23.543205260412822 23.308430044940234 27.16790786180223 14-15 7.137280865748492 41.792512818802976 23.872457375842778 27.197748939605752 16-17 25.73377376509618 5.016730532447706 42.35439673629585 26.895098966160262 18-19 25.93511173137791 42.13857882164657 4.960501654000681 26.965807792974843 20-21 25.986124970528067 4.238719096146381 42.51360472122527 27.261551212100283 22-23 25.825869094598357 4.048765036640462 42.33122405576685 27.794141812994326 24-25 7.96883000588248 23.594337578085742 22.79441569359667 45.64241672243511 26-27 42.689650409272886 6.555391756432026 6.947826936038163 43.80713089825693 28-29 9.224393949858415 22.10966655632057 23.23426794096563 45.431671552855384 30-31 44.823585168531835 22.691531849932485 23.445989554431986 9.0388934271037 32-33 44.39059184407382 22.784270203457563 6.005582401146965 26.81955555132165 34-35 6.919200459166784 41.52851573384521 24.734371539280435 26.817912267707573 36-37 25.502904325170107 4.718438832935214 42.33884508122346 27.439811760671223 38-39 44.87302657118157 4.466802098639885 42.43065462227102 8.229516707907528 40-41 25.7421330773939 23.790245563729634 25.903722632778507 24.563898726097964 42-43 26.335120305031545 4.322336034828086 43.833280541854904 25.509263118285457 44-45 7.238473794389496 4.697242855884027 63.005375204517364 25.058908145209113 46-47 24.29959203698103 44.098920910426756 23.18978020486269 8.41170684772953 48-49 7.784234479900737 23.16727436406115 42.16177531788012 26.886715838157997 50-51 27.615095346173174 5.9566887597019225 40.60256114086751 25.825654753257393 52-53 44.223929543619654 23.820039010124056 23.451490982183472 8.504540464072818 54-55 43.102995777475584 25.323953121167158 4.705625983886294 26.86742511747096 56-57 25.59264189992165 23.01942647020298 22.568095053240008 28.819836576635367 58-59 7.291154132858289 23.10306722458924 42.72130148062235 26.88447716193012 60-61 24.677880688083338 24.384637917935844 24.409049015101537 26.52843237887928 62-63 7.555841873248058 5.721556308661057 42.730327632647516 43.992274185443364 64-65 43.34601122672312 24.981673815347317 23.9581462808205 7.714168677109059 66-67 25.938160141560545 23.551659835528742 23.072726016990124 27.437454005920586 68-69 8.218180432540827 23.313860025578066 41.4667854276467 27.00117411423441 70-71 44.98741340014432 5.740727950825334 23.393380663076847 25.878477985953495 72-73 60.478981449947725 26.360746003129382 4.541130912546351 8.619141634376541 74-75 8.096839417848917 41.162777959041755 23.033382473070393 27.70700015003894 76 7.527810788990476 76.1893920088785 4.190373215906033 12.092423986224997 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.5 3 0.5 4 0.0 5 1.0 6 1.0 7 2.0 8 4.0 9 4.0 10 5.5 11 4.5 12 2.0 13 5.5 14 11.5 15 16.5 16 22.5 17 26.0 18 29.0 19 42.0 20 55.5 21 59.0 22 85.5 23 115.0 24 128.0 25 138.0 26 179.0 27 237.5 28 310.5 29 366.0 30 486.5 31 911.0 32 2523.0 33 3831.0 34 9856.5 35 12028.5 36 13596.5 37 19018.0 38 15631.5 39 17261.5 40 44324.0 41 332251.5 42 598133.0 43 333217.0 44 46853.5 45 19869.0 46 14332.0 47 14078.0 48 18385.0 49 19322.0 50 15698.0 51 25650.5 52 64582.5 53 342836.0 54 592110.0 55 340958.0 56 57492.0 57 21391.5 58 17605.0 59 16965.5 60 20557.0 61 21987.5 62 19187.0 63 20229.0 64 22688.5 65 25099.0 66 26978.0 67 27864.0 68 28265.5 69 28194.5 70 26633.5 71 25545.0 72 24029.0 73 20717.0 74 17141.0 75 15361.0 76 13491.0 77 10006.0 78 7072.0 79 5753.0 80 4835.5 81 3234.0 82 2106.0 83 1662.0 84 1385.5 85 899.5 86 597.0 87 504.0 88 413.5 89 293.0 90 222.5 91 151.5 92 121.0 93 106.5 94 89.5 95 78.5 96 70.0 97 57.0 98 42.0 99 73.5 100 107.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 2099455.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 22.443920512887594 #Duplication Level Percentage of deduplicated Percentage of total 1 96.02426408228393 21.551609503713077 2 1.9803434857818107 0.8889334356620341 3 0.7494006882510245 0.5045846843822775 4 0.4162324949673871 0.37367556127715684 5 0.22967996514239933 0.2577459440529402 6 0.09640739201007845 0.1298255905877397 7 0.050384642330113534 0.07915802352691378 8 0.036687179745352475 0.06587233168373685 9 0.04149480335360413 0.08381754613495733 >10 0.26073933234944896 1.1642498749873835 >50 0.040079630547746194 0.6562863490074771 >100 0.04819205652302722 2.375334522646005 >500 0.012177685330270164 2.0630129658244196 >1k 0.010437421084781767 4.578253350574695 >5k 0.0015221238808893463 2.504154731407478 >10k+ 0.001957016418286302 62.72348558453172 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCAC 601394 28.645243646565415 No Hit TCATTGTTTGGCATAGTCACGTTCAGCACTGGATATGTGCTTCCTGATTT 598965 28.529546953852307 No Hit TCATTGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTG 13680 0.6515976765398639 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAA 13052 0.621685151622683 No Hit GCCTCCCTTAGGTCACTAATTGCCTCGTCAGGCACTCTGGAGTTTATCAC 11940 0.5687190246992672 No Hit TCATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTG 11938 0.5686237618810596 No Hit TCATTGTTTGGCATAGTCACGTTCAGCACTAGATATGTGCTTCCTGATTT 11762 0.5602406338787924 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAA 11745 0.559430899924028 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCGTCAAGCACTCTGGAGTTTATCAC 10750 0.5120376478657557 No Hit GATCTTTGGCGGCACGGAGCCGCGCATCACCTGTAATGCGTACCTGACCA 9288 0.44240052775601285 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTAACAC 9041 0.4306355697073765 No Hit TCATTGTTTGGCATAGTCACGTTCAGCACTGGATATGTGTTTCCTGATTT 8186 0.38991071492363494 No Hit CTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCACTG 7756 0.3694292090090047 No Hit GATCTCCCGAGCATCACCACATTACTGCGGTTATAGGTCCACGTCTTATC 6830 0.32532252417889407 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACCCTGGAGTTTATCAC 5115 0.2436346575658921 No Hit CATTGTTTGGCATAGTCACGTTCAGCACTGGATATGTGCTTCCTGATTTG 5095 0.24268202938381628 No Hit TGGCATAGTCACGTTCAGCACTGGATATGTGCTTCCTGATTTGGTCAACC 4871 0.23201259374456704 No Hit CCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCACT 4274 0.2035766425096037 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCGTCCGGCACTCTGGAGTTTATCAC 3750 0.17861778413921708 No Hit GATCTCGTCGAACTATAACCCGCAGACGCGGCAATACAGCGGTATCTGGG 3653 0.17399753745614932 No Hit ATTGTTTGGCATAGTCACGTTCAGCACTGGATATGTGCTTCCTGATTTGG 3372 0.16061311149798402 No Hit TCATTGTTTGGCATAGTCACGTTCAGCACTGGATATTTGCTTCCTGATTT 3063 0.14589500608491252 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCATCAGGCACCCTGGAGTTTATCAA 3002 0.14298949012958123 No Hit TCATTGTTTGGCATAGTCACGTTCAGCGCTGGATATTTGTATTCTGATTC 2999 0.1428465959022699 No Hit TGTTTGGCATAGTCACGTTCAGCACTGGATATGTGCTTCCTGATTTGGTC 2935 0.13979818571962724 No Hit TCATTGTTTGGCATAGTCACGTTCAGCACTGGGTATGTGCTTCCTGATTT 2545 0.12122193616914866 No Hit TTGTTTGGCATAGTCACGTTCAGCACTGGATATGTGCTTCCTGATTTGGT 2525 0.12026930798707285 No Hit GATCTCCATGGCATCACCACATTACTGCGGTTATAGGTCCACGTCTTATC 2317 0.11036197489348426 No Hit GGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCACTGAGGGTTTC 2157 0.10274094943687766 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0013813108640099454 0.0 0.0 2 0.0013813108640099454 0.0 0.0 3 0.0014289422731137366 0.0 0.0 4 0.0014289422731137366 0.0 0.0 5 0.0014289422731137366 0.0 0.0 6 0.0014289422731137366 0.0 0.0 7 0.0014289422731137366 0.0 0.0 8 0.0014289422731137366 0.0 0.0 9 0.0014289422731137366 0.0 0.0 10 0.0014289422731137366 0.0 0.0 11 0.0014765736822175278 0.0 0.0 12 0.0014765736822175278 0.0 0.0 13 0.0014765736822175278 0.0 0.0 14 0.0014765736822175278 0.0 0.0 15 0.001524205091321319 0.0 0.0 16 0.001524205091321319 0.0 0.0 17 0.001524205091321319 0.0 0.0 18 0.001524205091321319 0.0 0.0 19 0.001524205091321319 0.0 0.0 20 0.0015718365004251103 0.0 0.0 21 0.0015718365004251103 0.0 0.0 22 0.0016194679095289015 0.0 0.0 23 0.0016194679095289015 0.0 0.0 24 0.0016194679095289015 0.0 0.0 25 0.0016670993186326927 0.0 0.0 26 0.001714730727736484 0.0 0.0 27 0.0018099935459440664 0.0 0.0 28 0.00195288777325544 0.0 0.0 29 0.0020481505914630225 0.0 0.0 30 0.0020481505914630225 0.0 0.0 31 0.002095782000566814 0.0 0.0 32 0.0022386762278781874 0.0 0.0 33 0.0022863076369819785 0.0 0.0 34 0.002381570455189561 0.0 0.0 35 0.0024768332733971434 0.0 0.0 36 0.0027626217280198907 0.0 0.0 37 0.0029531473644350556 0.0 0.0 38 0.0031436730008502205 0.0 0.0 39 0.0033341986372653854 0.0 0.0 40 0.0037628813191995067 9.526281820758244E-5 0.0 41 0.004096301182926045 9.526281820758244E-5 0.0 42 0.004382089637548792 9.526281820758244E-5 0.0 43 0.00466787809217154 9.526281820758244E-5 0.0 44 0.005096560774105661 9.526281820758244E-5 0.0 45 0.005382349228728408 9.526281820758244E-5 0.0 46 0.005811031910662529 9.526281820758244E-5 0.0 47 0.006573134456323189 9.526281820758244E-5 0.0 48 0.00700181713825731 9.526281820758244E-5 0.0 49 0.0076210254566065954 9.526281820758244E-5 0.0 50 0.008430759411371046 9.526281820758244E-5 0.0 51 0.009049967729720332 9.526281820758244E-5 0.0 52 0.009764438866277201 9.526281820758244E-5 0.0 53 0.010859961275664399 1.4289422731137365E-4 0.0 54 0.012003115094155388 1.4289422731137365E-4 0.0 55 0.012765217639816048 1.4289422731137365E-4 0.0 56 0.013717845821891872 1.4289422731137365E-4 0.0 57 0.014765736822175279 1.4289422731137365E-4 0.0 58 0.015575470776939729 1.4289422731137365E-4 0.0 59 0.016432836140807972 1.4289422731137365E-4 0.0 60 0.017671252777506544 1.4289422731137365E-4 0.0 61 0.01910019505062028 1.4289422731137365E-4 0.0 62 0.02067203155104539 1.4289422731137365E-4 0.0 63 0.021815185369536378 1.4289422731137365E-4 0.0 64 0.023815704551895613 1.4289422731137365E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTGTTAG 25 2.8418126E-6 70.0 4 TAGTAAC 40 1.3460522E-10 70.0 14 TCATTGT 77115 0.0 69.918304 1 GCCTCCC 76995 0.0 69.604515 1 TTAGACT 1715 0.0 69.38776 63 ACTTAGA 1650 0.0 69.36363 61 CATTGTT 78020 0.0 69.25532 2 ACTCTGT 1935 0.0 69.095604 34 CTAGATA 2170 0.0 69.03226 29 CCTCCCT 78745 0.0 68.91993 2 TCTGTAG 1950 0.0 68.74359 36 CTTAGAC 1665 0.0 68.73875 62 ATTGTTT 78440 0.0 68.69709 3 TTTAAAC 470 0.0 68.510635 59 TAGATAT 2190 0.0 68.401825 30 TTGTTTG 78870 0.0 68.33587 4 CTCCCTT 79500 0.0 68.181755 3 TGTGTTT 2080 0.0 68.14904 36 TCCCTTA 79480 0.0 68.08883 4 TGTTTGG 79190 0.0 67.8741 5 >>END_MODULE