FastQCFastQC Report
Tue 25 Aug 2015
000000000-AHV5L l01n02 kokuya6.34200000033d2e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AHV5L l01n02 kokuya6.34200000033d2e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2099455
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCAC60139428.645243646565415No Hit
TCATTGTTTGGCATAGTCACGTTCAGCACTGGATATGTGCTTCCTGATTT59896528.529546953852307No Hit
TCATTGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTG136800.6515976765398639No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAA130520.621685151622683No Hit
GCCTCCCTTAGGTCACTAATTGCCTCGTCAGGCACTCTGGAGTTTATCAC119400.5687190246992672No Hit
TCATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTG119380.5686237618810596No Hit
TCATTGTTTGGCATAGTCACGTTCAGCACTAGATATGTGCTTCCTGATTT117620.5602406338787924No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAA117450.559430899924028No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCGTCAAGCACTCTGGAGTTTATCAC107500.5120376478657557No Hit
GATCTTTGGCGGCACGGAGCCGCGCATCACCTGTAATGCGTACCTGACCA92880.44240052775601285No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTAACAC90410.4306355697073765No Hit
TCATTGTTTGGCATAGTCACGTTCAGCACTGGATATGTGTTTCCTGATTT81860.38991071492363494No Hit
CTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCACTG77560.3694292090090047No Hit
GATCTCCCGAGCATCACCACATTACTGCGGTTATAGGTCCACGTCTTATC68300.32532252417889407No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACCCTGGAGTTTATCAC51150.2436346575658921No Hit
CATTGTTTGGCATAGTCACGTTCAGCACTGGATATGTGCTTCCTGATTTG50950.24268202938381628No Hit
TGGCATAGTCACGTTCAGCACTGGATATGTGCTTCCTGATTTGGTCAACC48710.23201259374456704No Hit
CCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCACT42740.2035766425096037No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCGTCCGGCACTCTGGAGTTTATCAC37500.17861778413921708No Hit
GATCTCGTCGAACTATAACCCGCAGACGCGGCAATACAGCGGTATCTGGG36530.17399753745614932No Hit
ATTGTTTGGCATAGTCACGTTCAGCACTGGATATGTGCTTCCTGATTTGG33720.16061311149798402No Hit
TCATTGTTTGGCATAGTCACGTTCAGCACTGGATATTTGCTTCCTGATTT30630.14589500608491252No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCATCAGGCACCCTGGAGTTTATCAA30020.14298949012958123No Hit
TCATTGTTTGGCATAGTCACGTTCAGCGCTGGATATTTGTATTCTGATTC29990.1428465959022699No Hit
TGTTTGGCATAGTCACGTTCAGCACTGGATATGTGCTTCCTGATTTGGTC29350.13979818571962724No Hit
TCATTGTTTGGCATAGTCACGTTCAGCACTGGGTATGTGCTTCCTGATTT25450.12122193616914866No Hit
TTGTTTGGCATAGTCACGTTCAGCACTGGATATGTGCTTCCTGATTTGGT25250.12026930798707285No Hit
GATCTCCATGGCATCACCACATTACTGCGGTTATAGGTCCACGTCTTATC23170.11036197489348426No Hit
GGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCACTGAGGGTTTC21570.10274094943687766No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTTAG252.8418126E-670.04
TAGTAAC401.3460522E-1070.014
TCATTGT771150.069.9183041
GCCTCCC769950.069.6045151
TTAGACT17150.069.3877663
ACTTAGA16500.069.3636361
CATTGTT780200.069.255322
ACTCTGT19350.069.09560434
CTAGATA21700.069.0322629
CCTCCCT787450.068.919932
TCTGTAG19500.068.7435936
CTTAGAC16650.068.7387562
ATTGTTT784400.068.697093
TTTAAAC4700.068.51063559
TAGATAT21900.068.40182530
TTGTTTG788700.068.335874
CTCCCTT795000.068.1817553
TGTGTTT20800.068.1490436
TCCCTTA794800.068.088834
TGTTTGG791900.067.87415
Produced by FastQC (version 0.11.2)