##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AHV5L l01n02 kokuya5.34200000033d11.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2353186 Sequences flagged as poor quality 0 Sequence length 76 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.9385535185064 34.0 34.0 34.0 33.0 34.0 2 33.316999591192534 34.0 34.0 34.0 34.0 34.0 3 33.26268131800886 34.0 34.0 34.0 34.0 34.0 4 33.37367976862007 34.0 34.0 34.0 34.0 34.0 5 33.43819995529465 34.0 34.0 34.0 34.0 34.0 6 37.02459346604986 38.0 38.0 38.0 37.0 38.0 7 37.023151591076946 38.0 38.0 38.0 37.0 38.0 8 37.131328760242496 38.0 38.0 38.0 37.0 38.0 9 37.21010663840428 38.0 38.0 38.0 37.0 38.0 10-11 37.0650630676878 38.0 38.0 38.0 37.0 38.0 12-13 37.020580396109786 38.0 38.0 38.0 37.0 38.0 14-15 36.87842716215378 38.0 38.0 38.0 36.5 38.0 16-17 37.01713251736157 38.0 38.0 38.0 37.0 38.0 18-19 36.26300640918312 38.0 38.0 38.0 33.5 38.0 20-21 36.744884169802134 38.0 38.0 38.0 35.0 38.0 22-23 36.79634355295332 38.0 37.5 38.0 36.0 38.0 24-25 36.939989444098345 38.0 38.0 38.0 36.5 38.0 26-27 36.44221132541159 38.0 38.0 38.0 34.5 38.0 28-29 36.77653763875869 38.0 38.0 38.0 36.5 38.0 30-31 36.4091370167934 38.0 38.0 38.0 34.5 38.0 32-33 36.15354374877294 38.0 37.5 38.0 32.5 38.0 34-35 36.30344116444684 38.0 37.0 38.0 34.0 38.0 36-37 36.365659790598784 38.0 37.5 38.0 34.0 38.0 38-39 36.83333637884978 38.0 38.0 38.0 36.0 38.0 40-41 35.65290950226628 38.0 37.0 38.0 31.0 38.0 42-43 36.21507160929905 38.0 37.0 38.0 34.0 38.0 44-45 36.39134475557818 38.0 37.0 38.0 34.0 38.0 46-47 36.06217719296308 38.0 37.0 38.0 34.0 38.0 48-49 36.34806853346909 38.0 37.0 38.0 34.0 38.0 50-51 36.292057661400335 38.0 37.5 38.0 34.0 38.0 52-53 35.68397334507344 38.0 37.0 38.0 30.5 38.0 54-55 36.18049720676564 38.0 37.0 38.0 34.0 38.0 56-57 36.48449846293494 38.0 37.5 38.0 34.0 38.0 58-59 36.458391304384776 38.0 37.5 38.0 34.5 38.0 60-61 36.44659708157366 38.0 37.5 38.0 34.0 38.0 62-63 36.239779175976736 38.0 37.0 38.0 34.0 38.0 64-65 35.77352151508636 38.0 37.0 38.0 30.5 38.0 66-67 36.31915857904985 38.0 37.0 38.0 34.0 38.0 68-69 36.503409420249824 38.0 38.0 38.0 35.0 38.0 70-71 36.52534011336121 38.0 38.0 38.0 35.5 38.0 72-73 36.109258681634174 38.0 37.0 38.0 33.5 38.0 74-75 36.04288929986835 38.0 37.0 38.0 33.0 38.0 76 34.134051876902205 37.0 34.0 38.0 24.0 38.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.23493457799047945 1101 2 0.030202931978131176 1101 3 0.0672201811195805 1101 4 0.10510533734073846 1101 5 0.06163267933129646 1101 6 0.07944688795919319 1101 7 0.17153414559842162 1101 8 0.20478081724909458 1101 9 0.2152185309293344 1101 10-11 0.1648168991045722 1101 12-13 0.17974837779270558 1101 14-15 0.22824857204903282 1101 16-17 0.19613076533951812 1101 18-19 0.7407285404767237 1101 20-21 0.263100805086637 1101 22-23 0.21383915837887457 1101 24-25 0.21833693125241638 1101 26-27 0.3160583728651929 1101 28-29 0.21524348236700064 1101 30-31 0.5678795180145073 1101 32-33 0.6243842913562503 1101 34-35 0.4634970323508867 1101 36-37 0.3110502317749422 1101 38-39 0.2559950085191005 1101 40-41 0.8941941151631951 1101 42-43 0.4613248469096547 1101 44-45 0.3758095725250712 1101 46-47 0.32244433472599354 1101 48-49 0.31119156105565793 1101 50-51 0.2720322364826444 1101 52-53 0.8303710622079592 1101 54-55 0.4859313165629544 1101 56-57 0.28494348412379367 1101 58-59 0.23804114546384625 1101 60-61 0.2878004021976537 1101 62-63 0.4219921405475233 1101 64-65 0.8284730173470081 1101 66-67 0.4109016131446239 1101 68-69 0.3531534889100598 1101 70-71 0.2922109198286975 1101 72-73 0.3503125175837809 1101 74-75 0.5920313182816201 1101 76 0.6414030427130584 1102 1 0.15782098464755023 1102 2 0.07178733436467866 1102 3 0.16799028943751892 1102 4 0.11763343986531538 1102 5 0.08511506580444461 1102 6 0.15471800732325391 1102 7 0.2084155630307265 1102 8 0.19579038256516412 1102 9 0.10730389300982068 1102 10-11 0.16844945125284028 1102 12-13 0.20457492764860064 1102 14-15 0.20086617891097092 1102 16-17 0.23040074131692734 1102 18-19 0.8071084269363595 1102 20-21 0.40591323943996827 1102 22-23 0.25198293596834276 1102 24-25 0.19872006885416482 1102 26-27 0.3613206894473322 1102 28-29 0.26430943449493327 1102 30-31 0.6260786816201289 1102 32-33 0.625580559882529 1102 34-35 0.5338797013210836 1102 36-37 0.35817846820976484 1102 38-39 0.25915801415545303 1102 40-41 0.9491435519918738 1102 42-43 0.4093035423875193 1102 44-45 0.39135796107729703 1102 46-47 0.42787721260607725 1102 48-49 0.3984726291330105 1102 50-51 0.4519269078960093 1102 52-53 0.7293315407851324 1102 54-55 0.4875876245852453 1102 56-57 0.26696979148072586 1102 58-59 0.38149030684640906 1102 60-61 0.35037255008359125 1102 62-63 0.4316739711761812 1102 64-65 0.9358404514457277 1102 66-67 0.5636441470562872 1102 68-69 0.3283580763182954 1102 70-71 0.29432775341998507 1102 72-73 0.37201583401121496 1102 74-75 0.5825281909594651 1102 76 0.9515154110662678 1103 1 0.19605196252813784 1103 2 0.10602440816634129 1103 3 0.07645564127932403 1103 4 0.10991701719238733 1103 5 0.027025160704070572 1103 6 0.042606222464570465 1103 7 0.19637549573516822 1103 8 0.17320361481023383 1103 9 0.15584904137396904 1103 10-11 0.20124450607514888 1103 12-13 0.12583708151830564 1103 14-15 0.18701823915613147 1103 16-17 0.19262699772187375 1103 18-19 0.739571865926905 1103 20-21 0.3585602774598158 1103 22-23 0.3115030569333257 1103 24-25 0.1804591282929593 1103 26-27 0.2995501510153744 1103 28-29 0.24576837816441355 1103 30-31 0.5367924119480207 1103 32-33 0.5936779756120956 1103 34-35 0.4853881408209446 1103 36-37 0.21310213465236671 1103 38-39 0.22503694687934228 1103 40-41 0.8323172699761798 1103 42-43 0.47973090445426436 1103 44-45 0.3131449802184747 1103 46-47 0.42816015710240407 1103 48-49 0.3470065688458561 1103 50-51 0.33141242534499327 1103 52-53 0.7393571093183411 1103 54-55 0.4506841524965779 1103 56-57 0.2626879890434495 1103 58-59 0.3062734073822071 1103 60-61 0.24285069788024316 1103 62-63 0.277311371967194 1103 64-65 0.728194543050023 1103 66-67 0.4705198261123087 1103 68-69 0.34916400964930716 1103 70-71 0.37580891910937453 1103 72-73 0.36851140021854434 1103 74-75 0.6287125584260878 1103 76 0.9378501036914741 2116 1 0.06319372448497518 2116 2 0.013164945981387177 2116 3 0.10268073800165212 2116 4 0.06077431897262642 2116 5 0.148634112377934 2116 6 0.07906803360860692 2116 7 0.12349777876417534 2116 8 0.06005180427084156 2116 9 0.09047807131411645 2116 10-11 0.10782127635175698 2116 12-13 0.05520959580105256 2116 14-15 0.11441013027877034 2116 16-17 0.09598477183014609 2116 18-19 0.6561620332477958 2116 20-21 0.24645338384578963 2116 22-23 0.1914555997424543 2116 24-25 0.08485472512803227 2116 26-27 0.206918802285756 2116 28-29 0.1111226447410445 2116 30-31 0.4827132078046148 2116 32-33 0.5177333666577368 2116 34-35 0.3400847657695323 2116 36-37 0.10104574259675303 2116 38-39 0.0963818287876208 2116 40-41 0.7415941607428209 2116 42-43 0.2754241663816188 2116 44-45 0.1527295062669154 2116 46-47 0.07763032466994702 2116 48-49 0.10406655253576957 2116 50-51 0.1218463025326244 2116 52-53 0.5740714894615309 2116 54-55 0.32578060933121833 2116 56-57 0.1176567628650318 2116 58-59 0.09117249647569281 2116 60-61 0.07837037661609969 2116 62-63 0.12573913769546863 2116 64-65 0.6428298946776039 2116 66-67 0.31828015193956816 2116 68-69 0.2689657791850877 2116 70-71 0.17384305245324327 2116 72-73 0.10497043077932489 2116 74-75 0.4348729748992213 2116 76 0.6882537878558139 1117 1 0.29651302544453273 1117 2 0.1399873168412782 1117 3 0.05513361424553409 1117 4 0.07837622775584663 1117 5 0.023673617656307044 1117 6 -0.0019833320116191544 1117 7 -0.6333679519024642 1117 8 -0.0024760576843831927 1117 9 0.09434923198447365 1117 10-11 0.13356964568740892 1117 12-13 0.05479240465587765 1117 14-15 -0.544172030914865 1117 16-17 -0.2126151428458627 1117 18-19 0.024607259546634452 1117 20-21 0.12725533914238696 1117 22-23 0.1903689371295485 1117 24-25 0.2002858677202397 1117 26-27 0.2537482123457764 1117 28-29 0.13633845239839104 1117 30-31 0.5342859472425516 1117 32-33 0.5003676194158615 1117 34-35 0.48350029499705016 1117 36-37 0.28424185327463647 1117 38-39 0.13649609753786507 1117 40-41 0.8387313425259819 1117 42-43 0.35053082564068205 1117 44-45 0.24121967515122833 1117 46-47 0.22772399333250348 1117 48-49 0.213080637155322 1117 50-51 0.1917126746982234 1117 52-53 0.8541237445531991 1117 54-55 0.43590654750847335 1117 56-57 0.27947292214575015 1117 58-59 0.22072192262140078 1117 60-61 0.21192717426560392 1117 62-63 0.0849635995121858 1117 64-65 0.7921276949518159 1117 66-67 0.5200929984541389 1117 68-69 0.3255558387526847 1117 70-71 0.2411559101225791 1117 72-73 0.4194707608291992 1117 74-75 0.46510101029252837 1117 76 0.62970190144204 2117 1 0.08894018838844886 2117 2 0.016748106538692298 2117 3 0.013226188588163268 2117 4 0.10289016362911241 2117 5 0.08334439186570819 2117 6 -0.10341883892861148 2117 7 0.2332439573348637 2117 8 0.18010375842616355 2117 9 0.13447562670621238 2117 10-11 0.12908384216484592 2117 12-13 0.12936433712238937 2117 14-15 0.001620269708510591 2117 16-17 0.05162780033144543 2117 18-19 0.4847972137366554 2117 20-21 0.304811989997404 2117 22-23 0.19141003835700587 2117 24-25 0.1942098186017276 2117 26-27 0.28958654813634865 2117 28-29 0.14475655847028435 2117 30-31 0.5545620100079844 2117 32-33 0.40810328881723024 2117 34-35 0.33017520696251523 2117 36-37 0.27872292620340744 2117 38-39 0.16645436069874364 2117 40-41 0.7232740325562688 2117 42-43 0.29011304931284343 2117 44-45 0.23631514250572394 2117 46-47 0.27032184182608177 2117 48-49 0.2648495063742331 2117 50-51 0.23095479181801437 2117 52-53 0.8037055218148197 2117 54-55 0.4132067273061182 2117 56-57 0.20284376697740925 2117 58-59 0.3078826605148208 2117 60-61 0.21576599369588223 2117 62-63 0.2817607708196519 2117 64-65 0.765132983869961 2117 66-67 0.43898175985104615 2117 68-69 0.28568099551973347 2117 70-71 0.3667074874002054 2117 72-73 0.29588737875281623 2117 74-75 0.395574082684476 2117 76 0.7455223796795565 1116 1 0.26784853307012213 1116 2 -0.004781932399076538 1116 3 0.026118530932627948 1116 4 0.05994945929381146 1116 5 0.05134128815365813 1116 6 -0.0446941253073021 1116 7 0.16053918851900306 1116 8 0.1233149380723475 1116 9 0.08145060695460415 1116 10-11 0.06662634935474898 1116 12-13 0.07640587597298065 1116 14-15 0.12158832157945909 1116 16-17 0.003941016081839166 1116 18-19 0.5824441806277747 1116 20-21 0.2292344026937272 1116 22-23 0.2085670396936763 1116 24-25 0.11209260320945447 1116 26-27 0.049665366086991014 1116 28-29 0.08940277299396371 1116 30-31 0.43633783278225735 1116 32-33 0.3461128842508785 1116 34-35 0.29856104894650315 1116 36-37 0.1713022507062405 1116 38-39 0.19896264223809368 1116 40-41 0.7552384576705791 1116 42-43 0.12567069652319418 1116 44-45 0.03972282513545622 1116 46-47 0.22160349741142937 1116 48-49 0.19708833356061461 1116 50-51 0.26276391352719486 1116 52-53 0.7417911000775277 1116 54-55 0.28272339797808144 1116 56-57 -0.0930272828971681 1116 58-59 0.10693831371583684 1116 60-61 0.002339454758903514 1116 62-63 0.208706127581614 1116 64-65 0.6784391283088027 1116 66-67 0.39621029892310133 1116 68-69 0.17475745270154164 1116 70-71 0.2552267371689183 1116 72-73 0.2627062466832726 1116 74-75 0.45466143270183323 1116 76 0.6360061880724572 2118 1 0.072240943393723 2118 2 0.05942043197035218 2118 3 0.13274218401041793 2118 4 0.03422387206616406 2118 5 0.10298063520534839 2118 6 -0.15169465356295575 2118 7 0.16261625582482253 2118 8 0.06878669815223049 2118 9 0.04897504610068637 2118 10-11 0.0906867932044193 2118 12-13 0.06676366220411722 2118 14-15 0.15827094581786127 2118 16-17 0.120157747733046 2118 18-19 0.6058780844872587 2118 20-21 0.1575062781206782 2118 22-23 0.07054163131647329 2118 24-25 0.09749965936382665 2118 26-27 0.24053569809698416 2118 28-29 0.10766822053874137 2118 30-31 0.38299341216110605 2118 32-33 0.41468282774832943 2118 34-35 0.34148262962085596 2118 36-37 0.2282654791700338 2118 38-39 0.1051920477850743 2118 40-41 0.7880951335829778 2118 42-43 0.1888814727599808 2118 44-45 0.15876383331658417 2118 46-47 0.37643010219369444 2118 48-49 0.18360629422489438 2118 50-51 0.2565820420628597 2118 52-53 0.7317873528040906 2118 54-55 0.32789659235936597 2118 56-57 0.04161237160165143 2118 58-59 0.011179605768134593 2118 60-61 0.14271748084119196 2118 62-63 0.25874590993515056 2118 64-65 0.7486611329210149 2118 66-67 0.34948823710040244 2118 68-69 0.14566657180888143 2118 70-71 0.1943206568286442 2118 72-73 0.1415926760694859 2118 74-75 0.4420484852110249 2118 76 0.5029165865079861 1119 1 0.1551340438029314 1119 2 -0.00887769415028572 1119 3 0.056130456830423725 1119 4 0.040489924125495236 1119 5 0.08927584874373906 1119 6 -0.15292435851455366 1119 7 0.1534406021777528 1119 8 0.039935765279622615 1119 9 0.0456297661547751 1119 10-11 0.05173479409539539 1119 12-13 0.022401841578911785 1119 14-15 -0.0128096106091391 1119 16-17 -0.00336450453909265 1119 18-19 0.46501460786644344 1119 20-21 0.1560045109769419 1119 22-23 0.08568024249754558 1119 24-25 0.11227187173128783 1119 26-27 0.06546270943648125 1119 28-29 0.08482017774019823 1119 30-31 0.31248083378705616 1119 32-33 0.3424175214678442 1119 34-35 0.1488143219733331 1119 36-37 0.13640826868484623 1119 38-39 0.12456327143008394 1119 40-41 0.5456910214032646 1119 42-43 0.04419638102154266 1119 44-45 -0.024078576305647914 1119 46-47 0.16575354217406613 1119 48-49 -0.005778129582949987 1119 50-51 0.09538141167749359 1119 52-53 0.4620423901122521 1119 54-55 0.22904313678650112 1119 56-57 0.02521767520010343 1119 58-59 0.1868693829431649 1119 60-61 0.20512205432741126 1119 62-63 0.2482080232169821 1119 64-65 0.6449880133675805 1119 66-67 0.237545892166942 1119 68-69 0.16424024499139023 1119 70-71 0.17550536879706158 1119 72-73 0.08970569571550868 1119 74-75 0.393425401534202 1119 76 0.40306699025423853 2119 1 0.2080653823812213 2119 2 0.18006863335678958 2119 3 0.12100870656449558 2119 4 0.12997024712635152 2119 5 0.11127281279888024 2119 6 0.04110195403804795 2119 7 0.08919049800208967 2119 8 0.1422348840208869 2119 9 0.10484487901229045 2119 10-11 0.1645191351299573 2119 12-13 0.11497074933436835 2119 14-15 0.2790477227280235 2119 16-17 0.19410371774382895 2119 18-19 0.7331159984431324 2119 20-21 0.32868074781862333 2119 22-23 0.27954714475836795 2119 24-25 0.10136081003169295 2119 26-27 0.2306108056000582 2119 28-29 0.18455882215151576 2119 30-31 0.5237636485681421 2119 32-33 0.4383775760918809 2119 34-35 0.3793316791731769 2119 36-37 0.2597073677724069 2119 38-39 0.19784229172176282 2119 40-41 0.7561478562430537 2119 42-43 0.29610632334345155 2119 44-45 0.23881560463075147 2119 46-47 0.32818975873490075 2119 48-49 0.22991756398182872 2119 50-51 0.3085871424319464 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5 0.00647047984544713 2111 6 0.07457385855450127 2111 7 -0.12412793157147917 2111 8 -0.03400497676958736 2111 9 -0.04338126061962555 2111 10-11 -0.07347125006933197 2111 12-13 -0.016869052554014274 2111 14-15 -0.0769537141759784 2111 16-17 -0.019348780382060227 2111 18-19 0.476741589303316 2111 20-21 0.11529483059698009 2111 22-23 -0.04010313917162733 2111 24-25 -0.050551631882292725 2111 26-27 -0.2235950816233938 2111 28-29 -0.03486357373719784 2111 30-31 -12.165088915299265 2111 32-33 -10.4094565859535 2111 34-35 -5.411174821713772 2111 36-37 -1.28154641956705 2111 38-39 -0.26579616661062033 2111 40-41 -6.692009531480821 2111 42-43 -0.8818963510845279 2111 44-45 -0.1485430029174637 2111 46-47 -0.1552217773679132 2111 48-49 -0.19307040254305008 2111 50-51 -0.05937199171164309 2111 52-53 0.23045699963753918 2111 54-55 0.010537418887402339 2111 56-57 -0.024398252085852334 2111 58-59 -0.05896494439971178 2111 60-61 -0.060480857117376274 2111 62-63 -0.20428895494162447 2111 64-65 0.32504955447578254 2111 66-67 -0.08125237622915193 2111 68-69 -0.06658783855716166 2111 70-71 -0.11214635058537681 2111 72-73 -0.002747642412117557 2111 74-75 0.07155636395441434 2111 76 0.0788249546149018 2110 1 0.024962127276957347 2110 2 -0.12996033968590837 2110 3 -0.11239019067762257 2110 4 -0.044026992428825906 2110 5 -0.14082077663243808 2110 6 -0.04337948087463417 2110 7 -0.18338112399540307 2110 8 -0.29677573431731474 2110 9 -0.18366073309982767 2110 10-11 -0.14028004472177713 2110 12-13 -0.12229073577177019 2110 14-15 -0.10943066927428191 2110 16-17 -0.2025576345571949 2110 18-19 0.4665837370177144 2110 20-21 -0.012223807224032157 2110 22-23 -0.1984208119166624 2110 24-25 -0.22096768578312975 2110 26-27 -0.1384089509362454 2110 28-29 -0.09580039840408006 2110 30-31 -0.013258504860765186 2110 32-33 -0.4020888229065136 2110 34-35 -0.2878732284420664 2110 36-37 -0.16777299624079944 2110 38-39 -0.18881977986314524 2110 40-41 0.26529656319393524 2110 42-43 -0.25732473792749744 2110 44-45 -0.2664378302584751 2110 46-47 -0.3788837216184149 2110 48-49 -0.27978381590100554 2110 50-51 -0.5060549201984372 2110 52-53 0.2291953281602872 2110 54-55 -0.14045053465267898 2110 56-57 -0.20064109204438552 2110 58-59 -0.2756129809346888 2110 60-61 -0.421942320646032 2110 62-63 -0.21882064073859908 2110 64-65 0.4156418286246151 2110 66-67 -0.09330952796615577 2110 68-69 -0.4085354474632439 2110 70-71 -0.2701583910402974 2110 72-73 -0.35708157932071316 2110 74-75 -0.0674116395267248 2110 76 -0.6370057800933466 2109 1 -0.19316186848366357 2109 2 -0.026919714400740702 2109 3 -0.04153602402155343 2109 4 -0.08235381411108733 2109 5 -0.09899616357731844 2109 6 0.03567165549272033 2109 7 -0.11653351043842264 2109 8 -0.10856560540477034 2109 9 -0.049095824301581104 2109 10-11 -0.08064768114856946 2109 12-13 -0.0778826586742909 2109 14-15 -0.10189666197337743 2109 16-17 -0.09252020319199517 2109 18-19 0.3354178323187469 2109 20-21 0.02496909256301194 2109 22-23 -0.16036607916446144 2109 24-25 -0.1379400225122751 2109 26-27 -0.21024904026047153 2109 28-29 -0.1025259149606228 2109 30-31 0.13655390954065183 2109 32-33 0.04033105977887175 2109 34-35 0.03994749402235698 2109 36-37 -0.07283467154844914 2109 38-39 -0.29176457325663563 2109 40-41 0.05528945114007655 2109 42-43 0.016936870200929377 2109 44-45 -0.10041681763853205 2109 46-47 -0.2997265669816187 2109 48-49 -0.15045004207042467 2109 50-51 -0.21511833278615455 2109 52-53 0.16349787898673895 2109 54-55 -0.17424893608661307 2109 56-57 -0.05588652306636277 2109 58-59 -0.18805052638730757 2109 60-61 -0.3362296323606486 2109 62-63 -0.1204949396790127 2109 64-65 0.4818752193480691 2109 66-67 -0.2410338923840314 2109 68-69 -0.2073365725722951 2109 70-71 -0.3043118864540375 2109 72-73 -0.36074759732949957 2109 74-75 -0.10560070892081797 2109 76 -0.8946835412878755 2108 1 -0.08733338695823534 2108 2 -0.11472721247862694 2108 3 -0.03920721444933406 2108 4 -0.050109844527085556 2108 5 -0.0036700552786541607 2108 6 -0.02874566737668971 2108 7 0.047922800584572656 2108 8 0.03134874000883059 2108 9 -0.05030549532375517 2108 10-11 -0.09055736020998495 2108 12-13 -0.04268738386772242 2108 14-15 -0.003969552354519124 2108 16-17 -0.08322473552604492 2108 18-19 0.5540108271916324 2108 20-21 0.019996768766688433 2108 22-23 -0.0400251113626382 2108 24-25 -0.09780231029889563 2108 26-27 -0.20268450882593214 2108 28-29 -0.09118065821931509 2108 30-31 0.3254887867582923 2108 32-33 0.110524473235877 2108 34-35 0.07644178365999466 2108 36-37 -0.09225528167812058 2108 38-39 -0.006888236198513198 2108 40-41 0.4021628472028098 2108 42-43 -0.04028444321952662 2108 44-45 -0.15923691947639895 2108 46-47 -0.1357120384120023 2108 48-49 0.01646059165737057 2108 50-51 -0.07682374720533858 2108 52-53 0.3671606209785594 2108 54-55 -0.16640933827979154 2108 56-57 -0.15813791670121446 2108 58-59 -0.09099242661537943 2108 60-61 -0.1710411290530871 2108 62-63 -0.1862923248042918 2108 64-65 0.29486547924759066 2108 66-67 0.009914685123291633 2108 68-69 -0.011062985673326864 2108 70-71 -0.081234138030581 2108 72-73 -0.23556795756820037 2108 74-75 0.0753803132703652 2108 76 -0.7996634531220579 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 53.0 13 362.0 14 964.0 15 1431.0 16 2041.0 17 3384.0 18 5438.0 19 7843.0 20 8968.0 21 8970.0 22 8531.0 23 8171.0 24 8395.0 25 8660.0 26 9243.0 27 10093.0 28 11692.0 29 13894.0 30 17384.0 31 24896.0 32 38096.0 33 52689.0 34 80767.0 35 139275.0 36 344668.0 37 1537277.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.990039036438255 2.5427654252575023 52.31970613457669 5.147489403727542 2 3.177649365583511 2.3738455013755817 5.450440381678287 88.99806475136262 3 3.3999012402759496 48.755219519409 5.0579937157538755 42.78688552456117 4 3.825451961723383 2.0107207845023725 89.04094278990272 5.122884463871534 5 3.3347130231099453 2.4394586743249365 51.35123190432036 42.87459639824476 6 49.7860347630829 2.6975343215538423 6.302901683079876 41.21352923228338 7 3.4090377896179898 3.0417060104896083 53.01561372539187 40.53364247450053 8 4.352269646343298 4.160104641112093 88.89548042526175 2.5921452872828583 9 6.254754192826237 2.9804273865304314 87.86160549994773 2.9032129206956014 10-11 69.6016591973605 22.095788433213524 4.086523547224911 4.21602882220105 12-13 21.564041261506738 27.42932347889202 22.5956001778015 28.41103508179974 14-15 3.655894604166437 45.32096060404915 27.997170644394455 23.025974147389963 16-17 26.887759828589836 3.697561518723977 46.382648885383475 23.03202976730271 18-19 22.670243661147058 44.8356610994626 5.108074754821761 27.38602048456858 20-21 27.747742847356733 2.366663748636954 42.15399887641691 27.731594527589404 22-23 21.581506944202456 2.758834193302187 50.97431737227741 24.685341490217944 24-25 4.459188521434345 26.95286305459917 22.32530705180126 46.26264137216523 26-27 21.006690503853072 28.211582084884068 22.83440408025545 27.947323331007407 28-29 22.844964231471714 8.157748686249196 22.611536019677153 46.38575106260194 30-31 46.878742266867135 9.22721365841884 26.86615082700645 17.027893247707578 32-33 56.53726479759781 15.58210868159168 4.9852412856442285 22.895385235166284 34-35 3.6972640496756313 44.948508107731385 28.303776242082012 23.050451600510964 36-37 21.575238846398033 11.204214201512332 40.756425543922155 26.46412140816748 38-39 46.87865727571046 3.9357704830812357 46.675294685587964 2.5102775556203376 40-41 22.702986504254234 39.57755570532886 29.94725023861267 7.772207551804235 42-43 33.02630986245882 3.317523561673408 55.35076700269337 8.305399573174412 44-45 3.515276735455676 5.556976796564317 82.61106431875763 8.316682149222373 46-47 8.929850849019159 77.23307889814065 8.920119361580428 4.916950891259765 48-49 15.626304083060157 23.531204078215662 38.997937264627616 21.844554574096563 50-51 45.6045973416466 17.74891572531878 27.61258566046203 9.033901272572589 52-53 44.35291132957616 24.6874662691347 26.904481838664683 4.055140562624459 54-55 13.789326470580738 54.10806880544079 4.4124008896874285 27.690203834291044 56-57 20.60115519980146 27.23078413691055 10.401196505503602 41.76686415778438 58-59 2.1938767271265425 28.133942663265888 41.94358202029079 27.728598589316782 60-61 8.016280905971733 40.992870941778506 29.11437939882355 21.876468753426206 62-63 3.555201331301478 17.795724604854865 58.41301962530798 20.23605443853567 64-65 28.04187599280295 45.77513209750526 22.57420365410979 3.6087882555820063 66-67 21.65100846256947 23.267858979273207 27.083706940292863 27.99742561786446 68-69 4.822631955145067 27.749145201441788 45.0895509322255 22.338671911187642 70-71 41.13283012902508 5.918252955779951 26.47999350667563 26.468923408519345 72-73 31.946072261181225 59.537750097102396 3.9824731236714817 4.533704518044898 74-75 20.82279088860804 33.68968708805849 21.68774589004014 23.79977613329333 76 11.995184401063069 77.67584032881378 4.07583590927364 6.253139360849504 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.5 3 2.0 4 3.0 5 1.5 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 1.0 14 1.0 15 2.5 16 4.0 17 4.0 18 3.5 19 5.5 20 17.5 21 27.0 22 25.0 23 41.5 24 76.0 25 92.0 26 142.0 27 314.5 28 768.0 29 1099.0 30 2421.5 31 4887.5 32 22762.5 33 39494.0 34 138120.5 35 160842.0 36 282460.5 37 479984.0 38 282593.0 39 55798.0 40 30091.5 41 118462.0 42 203135.0 43 119950.0 44 31270.5 45 25233.5 46 24691.0 47 45325.0 48 201835.0 49 219692.0 50 101673.0 51 146727.5 52 128635.5 53 130251.0 54 195013.0 55 114565.5 56 21402.0 57 6637.0 58 4588.0 59 4079.5 60 3571.5 61 3682.0 62 3792.0 63 3915.0 64 4219.0 65 4541.5 66 4787.5 67 4892.0 68 4925.0 69 4892.5 70 4606.5 71 4386.0 72 4171.5 73 3640.0 74 2942.5 75 2562.0 76 2254.0 77 1706.0 78 1234.0 79 1002.0 80 831.0 81 560.0 82 359.5 83 259.0 84 223.5 85 150.5 86 98.0 87 83.0 88 66.5 89 44.0 90 38.5 91 33.0 92 27.0 93 22.5 94 17.5 95 18.5 96 20.0 97 25.5 98 33.0 99 72.0 100 109.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 2353186.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 7.937082625287012 #Duplication Level Percentage of deduplicated Percentage of total 1 87.70677521915886 6.9613592171193925 2 4.330228232455771 0.6873875853470397 3 2.5724705248395487 0.6125373332030083 4 1.4352634897700873 0.4556721962945185 5 1.026228283683464 0.4072629340001066 6 0.6643189929681425 0.3163652842041365 7 0.4039548616725226 0.22443561797868386 8 0.27083397637482587 0.17197053185776187 9 0.15796454013000558 0.11283998461895882 >10 0.9351078208914435 1.5086275127522872 >50 0.17826178043751403 1.0324153619696106 >100 0.21812579140351923 3.9286026619844026 >500 0.0469211623735701 2.612528131647455 >1k 0.04416109182736053 7.4255155074216965 >5k 0.004968122830577997 2.4654013630161637 >10k+ 0.004416109182735998 71.07707877658474 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TCATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTG 456603 19.403608554529903 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAA 339975 14.447434244466864 No Hit TCATTGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTG 235944 10.026576734690755 No Hit TCATTGTTTGGCATAGTCACGTTCAGCACTGGATATGTGCTTCCTGATTT 197434 8.390072013007046 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCAC 188002 7.989253718150627 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAA 181888 7.729435752209983 No Hit TCATTGTTTGGCATAGTCACGTTCAATGCTAGGTATGTGTAGTTTAAGTG 11953 0.5079496478391423 No Hit CTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAATG 10455 0.44429127149320113 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCATCCAGCACACTGGAGTTTAACAA 6792 0.2886299680518242 No Hit GCCTCCCTTAGGTCACTAATTGCCTCATCCGGCACACTGGAGTTTAACAA 6496 0.2760512768646422 No Hit CTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCACTG 6451 0.2741389758395639 No Hit ATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGG 6440 0.27367152447787807 No Hit TCATTGTTTGGCATAGTCACGTTCAGTGCTAGATATTTGTATTTTAATTG 6355 0.2700594003193968 No Hit TTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTCAAC 6325 0.2687845329693445 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGTAGTTTAACAA 6184 0.2627926564240991 No Hit TCATTGTTTGGCATAGTCGCGTTCAATGCTGGGTATGTGTAGTTTAAGTG 5883 0.25000148734524175 No Hit CTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAATG 5344 0.22709637062263674 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTAACAA 4835 0.2054661212500839 No Hit CATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGG 4704 0.1998992004881892 No Hit TGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTCAACC 4596 0.1953096780280012 No Hit TCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAATGA 4079 0.1733394640287678 No Hit TCATTGTTTGGCATAGTCACGTTCAGTGCTGGGTATGTGTAGTTTAAGTG 4059 0.17248955246206632 No Hit TCATTGTTTGGCATAGTCACGTTCAGCACTAGATATGTGCTTCCTGATTT 3982 0.1692173929302656 No Hit TGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTC 3887 0.16518031298843355 No Hit TGGCATAGTCACGTTCAGCACTGGATATGTGCTTCCTGATTTGGTCAACC 3806 0.16173817114329253 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCATCCAGCACCCTGGAGTTTAACAA 3694 0.15697866636976424 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGTAGTTTATCAC 3641 0.1547264007180053 No Hit CCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAAT 3525 0.14979691363113667 No Hit TCATTGTTTGGCATAGTCGCGTTCAGTGCTGGATATTTGTATTTTAATTG 3522 0.14966942689613147 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCGTCAAGCACTCTGGAGTTTATCAC 3390 0.14406001055590167 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGTAGTTTAACAA 3317 0.14095783333744125 No Hit CCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAATGAA 3100 0.13173629283873015 No Hit TTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGT 3093 0.1314388237903846 No Hit TTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTGGTGCAAC 2907 0.12353464622006081 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTAACAC 2902 0.12332216832838543 No Hit TCATTGTTTGGCATAGTCGCGTTCAGCACTGGATATGTGCTTCCTGATTT 2865 0.12174983192998769 No Hit TCATTGTTTGGCATAGTCACGTTCAGCACTGGATATGTGTTTCCTGATTT 2786 0.11839268124151682 No Hit TCATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTATTTTAAGTG 2719 0.11554547749306684 No Hit TTGGCATAGTCACGTTCAGCACTGGATATGTGCTTCCTGATTTGGTCAAC 2588 0.10997855673117213 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTATCAC 2533 0.10764129992274303 No Hit TCATTGTTTGGCATAGTCACGTTCAATGCTGGATATTTGTATTTTAATTG 2487 0.10568650331932962 No Hit TCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCACTGA 2470 0.10496407848763335 No Hit CCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCACT 2436 0.10351922882424083 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0020822833384186376 0.0 0.0 2 0.0020822833384186376 0.0 0.0 3 0.00220977007342386 0.0 0.0 4 0.00220977007342386 0.0 0.0 5 0.00220977007342386 0.0 0.0 6 0.00220977007342386 0.0 0.0 7 0.0022522656517589344 0.0 0.0 8 0.0022522656517589344 0.0 0.0 9 0.0022522656517589344 0.0 0.0 10 0.0022522656517589344 0.0 0.0 11 0.0022522656517589344 0.0 0.0 12 0.0022522656517589344 0.0 0.0 13 0.0022522656517589344 0.0 0.0 14 0.0022522656517589344 0.0 0.0 15 0.0022522656517589344 0.0 0.0 16 0.0022522656517589344 0.0 0.0 17 0.0022522656517589344 0.0 0.0 18 0.0022947612300940087 0.0 0.0 19 0.0022947612300940087 0.0 0.0 20 0.002337256808429083 0.0 0.0 21 0.002337256808429083 0.0 0.0 22 0.0024222479650992316 0.0 0.0 23 0.0024222479650992316 0.0 0.0 24 0.0024647435434343055 0.0 0.0 25 0.0024647435434343055 0.0 0.0 26 0.0024647435434343055 0.0 0.0 27 0.0024647435434343055 0.0 0.0 28 0.00250723912176938 0.0 0.0 29 0.002549734700104454 0.0 4.249557833507423E-5 30 0.0025922302784395284 0.0 4.249557833507423E-5 31 0.0025922302784395284 0.0 4.249557833507423E-5 32 0.002719717013444751 0.0 4.249557833507423E-5 33 0.0028047081701148995 0.0 4.249557833507423E-5 34 0.0029321949051201225 0.0 4.249557833507423E-5 35 0.0030171860617902706 0.0 4.249557833507423E-5 36 0.003272159531800716 0.0 4.249557833507423E-5 37 0.0035271330018111615 0.0 4.249557833507423E-5 38 0.0037396108934865326 0.0 4.249557833507423E-5 39 0.003994584363496978 0.0 4.249557833507423E-5 40 0.004292053411842498 0.0 4.249557833507423E-5 41 0.004547026881852943 0.0 4.249557833507423E-5 42 0.005184460556879057 0.0 4.249557833507423E-5 43 0.005439434026889502 0.0 4.249557833507423E-5 44 0.005736903075235022 0.0 4.249557833507423E-5 45 0.006161858858585764 0.0 4.249557833507423E-5 46 0.006714301376941729 0.0 4.249557833507423E-5 47 0.007054266003622323 0.0 4.249557833507423E-5 48 0.007394230630302917 0.0 4.249557833507423E-5 49 0.007946673148658882 0.0 4.249557833507423E-5 50 0.008499115667014848 0.0 4.249557833507423E-5 51 0.008924071450365589 0.0 4.249557833507423E-5 52 0.009476513968721555 0.0 4.249557833507423E-5 53 0.010411416692093188 0.0 4.249557833507423E-5 54 0.011261328258794672 0.0 4.249557833507423E-5 55 0.012153735403831231 0.0 4.249557833507423E-5 56 0.012918655813862567 0.0 4.249557833507423E-5 57 0.01364108064555883 0.0 4.249557833507423E-5 58 0.014321009898920017 0.0 4.249557833507423E-5 59 0.015085930308951353 0.0 4.249557833507423E-5 60 0.015723363983977466 0.0 4.249557833507423E-5 61 0.017125718069034918 0.0 4.249557833507423E-5 62 0.018145611949076698 0.0 4.249557833507423E-5 63 0.01997292181748489 0.0 4.249557833507423E-5 64 0.021120302432531896 0.0 4.249557833507423E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATGCTA 15 0.0022251944 70.00001 25 ACCATAG 15 0.0022251944 70.00001 10 ATGTGAA 45 5.456968E-12 70.00001 35 TCCTATG 15 0.0022251944 70.00001 22 TCCTAGG 15 0.0022251944 70.00001 4 TAGTTAT 15 0.0022251944 70.00001 30 CTAAAAC 15 0.0022251944 70.00001 70 CGCTAGA 15 0.0022251944 70.00001 27 GTGAGTC 45 5.456968E-12 70.00001 48 GCGCTAG 30 1.02476406E-7 70.00001 26 TATGTAA 15 0.0022251944 70.00001 49 TGTAAGA 15 0.0022251944 70.00001 43 CTAGATA 1320 0.0 70.0 29 GCTATAT 20 7.930338E-5 70.0 28 AGTGCTA 780 0.0 70.0 25 TGTCTGT 20 7.930338E-5 70.0 32 TCCCGTA 25 2.84192E-6 70.0 4 GACTCCC 20 7.930338E-5 70.0 1 TCATTGT 108435 0.0 69.86443 1 GCCTCCC 88435 0.0 69.67547 1 >>END_MODULE