FastQCFastQC Report
Tue 25 Aug 2015
000000000-AHV5L l01n02 kokuya5.34200000033d11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AHV5L l01n02 kokuya5.34200000033d11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2353186
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTG45660319.403608554529903No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAA33997514.447434244466864No Hit
TCATTGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTG23594410.026576734690755No Hit
TCATTGTTTGGCATAGTCACGTTCAGCACTGGATATGTGCTTCCTGATTT1974348.390072013007046No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCAC1880027.989253718150627No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAA1818887.729435752209983No Hit
TCATTGTTTGGCATAGTCACGTTCAATGCTAGGTATGTGTAGTTTAAGTG119530.5079496478391423No Hit
CTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAATG104550.44429127149320113No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCATCCAGCACACTGGAGTTTAACAA67920.2886299680518242No Hit
GCCTCCCTTAGGTCACTAATTGCCTCATCCGGCACACTGGAGTTTAACAA64960.2760512768646422No Hit
CTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCACTG64510.2741389758395639No Hit
ATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGG64400.27367152447787807No Hit
TCATTGTTTGGCATAGTCACGTTCAGTGCTAGATATTTGTATTTTAATTG63550.2700594003193968No Hit
TTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTCAAC63250.2687845329693445No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGTAGTTTAACAA61840.2627926564240991No Hit
TCATTGTTTGGCATAGTCGCGTTCAATGCTGGGTATGTGTAGTTTAAGTG58830.25000148734524175No Hit
CTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAATG53440.22709637062263674No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTAACAA48350.2054661212500839No Hit
CATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGG47040.1998992004881892No Hit
TGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTCAACC45960.1953096780280012No Hit
TCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAATGA40790.1733394640287678No Hit
TCATTGTTTGGCATAGTCACGTTCAGTGCTGGGTATGTGTAGTTTAAGTG40590.17248955246206632No Hit
TCATTGTTTGGCATAGTCACGTTCAGCACTAGATATGTGCTTCCTGATTT39820.1692173929302656No Hit
TGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTC38870.16518031298843355No Hit
TGGCATAGTCACGTTCAGCACTGGATATGTGCTTCCTGATTTGGTCAACC38060.16173817114329253No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCATCCAGCACCCTGGAGTTTAACAA36940.15697866636976424No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGTAGTTTATCAC36410.1547264007180053No Hit
CCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAAT35250.14979691363113667No Hit
TCATTGTTTGGCATAGTCGCGTTCAGTGCTGGATATTTGTATTTTAATTG35220.14966942689613147No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCGTCAAGCACTCTGGAGTTTATCAC33900.14406001055590167No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGTAGTTTAACAA33170.14095783333744125No Hit
CCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAATGAA31000.13173629283873015No Hit
TTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGT30930.1314388237903846No Hit
TTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTGGTGCAAC29070.12353464622006081No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTAACAC29020.12332216832838543No Hit
TCATTGTTTGGCATAGTCGCGTTCAGCACTGGATATGTGCTTCCTGATTT28650.12174983192998769No Hit
TCATTGTTTGGCATAGTCACGTTCAGCACTGGATATGTGTTTCCTGATTT27860.11839268124151682No Hit
TCATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTATTTTAAGTG27190.11554547749306684No Hit
TTGGCATAGTCACGTTCAGCACTGGATATGTGCTTCCTGATTTGGTCAAC25880.10997855673117213No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTATCAC25330.10764129992274303No Hit
TCATTGTTTGGCATAGTCACGTTCAATGCTGGATATTTGTATTTTAATTG24870.10568650331932962No Hit
TCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCACTGA24700.10496407848763335No Hit
CCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCACT24360.10351922882424083No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGCTA150.002225194470.0000125
ACCATAG150.002225194470.0000110
ATGTGAA455.456968E-1270.0000135
TCCTATG150.002225194470.0000122
TCCTAGG150.002225194470.000014
TAGTTAT150.002225194470.0000130
CTAAAAC150.002225194470.0000170
CGCTAGA150.002225194470.0000127
GTGAGTC455.456968E-1270.0000148
GCGCTAG301.02476406E-770.0000126
TATGTAA150.002225194470.0000149
TGTAAGA150.002225194470.0000143
CTAGATA13200.070.029
GCTATAT207.930338E-570.028
AGTGCTA7800.070.025
TGTCTGT207.930338E-570.032
TCCCGTA252.84192E-670.04
GACTCCC207.930338E-570.01
TCATTGT1084350.069.864431
GCCTCCC884350.069.675471
Produced by FastQC (version 0.11.2)