##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AHV5L l01n02 kokuya4.34200000033d04.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2459974 Sequences flagged as poor quality 0 Sequence length 76 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.04163661892361 34.0 34.0 34.0 33.0 34.0 2 33.41256818161493 34.0 34.0 34.0 34.0 34.0 3 33.33775560229498 34.0 34.0 34.0 34.0 34.0 4 33.49982764045474 34.0 34.0 34.0 34.0 34.0 5 33.51331152280471 34.0 34.0 34.0 34.0 34.0 6 37.16227325979868 38.0 38.0 38.0 37.0 38.0 7 37.12905868110801 38.0 38.0 38.0 37.0 38.0 8 37.25332137656739 38.0 38.0 38.0 37.0 38.0 9 37.30419955658068 38.0 38.0 38.0 37.0 38.0 10-11 37.141187671089206 38.0 38.0 38.0 37.0 38.0 12-13 37.09547560258767 38.0 38.0 38.0 37.0 38.0 14-15 36.9852429334619 38.0 38.0 38.0 37.0 38.0 16-17 37.14329765273942 38.0 38.0 38.0 37.0 38.0 18-19 36.40924456925154 38.0 38.0 38.0 34.5 38.0 20-21 36.88492134469714 38.0 38.0 38.0 36.0 38.0 22-23 36.89355476927805 38.0 37.5 38.0 36.5 38.0 24-25 37.06510109456441 38.0 38.0 38.0 37.0 38.0 26-27 36.40809252455514 38.0 38.0 38.0 35.0 38.0 28-29 36.82011191988208 38.0 38.0 38.0 36.5 38.0 30-31 36.45447695788655 38.0 38.0 38.0 34.5 38.0 32-33 36.20281271265469 38.0 38.0 38.0 32.5 38.0 34-35 36.39450254352282 38.0 37.0 38.0 34.0 38.0 36-37 36.39446941309136 38.0 37.5 38.0 34.5 38.0 38-39 36.933560679909625 38.0 38.0 38.0 36.0 38.0 40-41 35.62074680464103 38.0 37.0 38.0 29.5 38.0 42-43 36.181044799660484 38.0 37.0 38.0 34.0 38.0 44-45 36.423097561193735 38.0 37.0 38.0 34.0 38.0 46-47 36.012369846185365 38.0 37.0 38.0 34.0 38.0 48-49 36.38145606416978 38.0 37.0 38.0 34.0 38.0 50-51 36.32562051468837 38.0 37.5 38.0 34.0 38.0 52-53 35.72961421543479 38.0 37.0 38.0 30.5 38.0 54-55 36.18713470142367 38.0 37.0 38.0 34.0 38.0 56-57 36.51221882832908 38.0 37.5 38.0 34.0 38.0 58-59 36.54122624873271 38.0 38.0 38.0 35.0 38.0 60-61 36.493634892076095 38.0 37.5 38.0 34.5 38.0 62-63 36.280537517876205 38.0 37.0 38.0 34.0 38.0 64-65 35.80759146234879 38.0 37.0 38.0 30.5 38.0 66-67 36.42501119930536 38.0 37.5 38.0 35.0 38.0 68-69 36.654890254937655 38.0 38.0 38.0 36.0 38.0 70-71 36.62290455102371 38.0 38.0 38.0 35.5 38.0 72-73 36.11276643574281 38.0 37.0 38.0 33.0 38.0 74-75 36.08595192469514 38.0 37.0 38.0 33.0 38.0 76 34.15918826784348 37.0 34.0 38.0 24.0 38.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.15143587272703485 1101 2 0.051944692483132826 1101 3 0.01115628843031402 1101 4 0.020610025172089763 1101 5 0.05100995168852762 1101 6 0.0946212586427535 1101 7 0.127305300889887 1101 8 0.14283224072831757 1101 9 0.12542825771011934 1101 10-11 0.12081049999881088 1101 12-13 0.14895858695736308 1101 14-15 0.14164921150213416 1101 16-17 0.12621600348338546 1101 18-19 0.7138899468206361 1101 20-21 0.2803189246505724 1101 22-23 0.21845821619068317 1101 24-25 0.16702856752021944 1101 26-27 0.2829514993867619 1101 28-29 0.15381193287274186 1101 30-31 0.5450366121129449 1101 32-33 0.6113539348103174 1101 34-35 0.39831675974704694 1101 36-37 0.3036746621912201 1101 38-39 0.1681487318999615 1101 40-41 0.8327400583923676 1101 42-43 0.4140481440466175 1101 44-45 0.23604717986228252 1101 46-47 0.27525097286358147 1101 48-49 0.2637331644061547 1101 50-51 0.2769018452213672 1101 52-53 0.7728044809290822 1101 54-55 0.42613836963064955 1101 56-57 0.1735147555044989 1101 58-59 0.14940996679523266 1101 60-61 0.22131312748895482 1101 62-63 0.34241141043411005 1101 64-65 0.8076259958092535 1101 66-67 0.29206286757140987 1101 68-69 0.2131307039528636 1101 70-71 0.2276865216249533 1101 72-73 0.18960077023467647 1101 74-75 0.49850928393930616 1101 76 0.5227226212918765 1102 1 0.08234360890445203 1102 2 0.07624094595731634 1102 3 0.11429570650251009 1102 4 0.09125719351428074 1102 5 0.05732498940027142 1102 6 0.12157465813474033 1102 7 0.21492535060197326 1102 8 0.1488818819906541 1102 9 0.08038781767513825 1102 10-11 0.12830753896512448 1102 12-13 0.12167561290237217 1102 14-15 0.17254676158805182 1102 16-17 0.17101369586686133 1102 18-19 0.802000433628713 1102 20-21 0.3263282560074643 1102 22-23 0.26738134082457776 1102 24-25 0.1501659181235553 1102 26-27 0.30595571521861586 1102 28-29 0.26060779530637035 1102 30-31 0.5487450170153423 1102 32-33 0.5806163345251747 1102 34-35 0.4222911447259179 1102 36-37 0.27909555300430355 1102 38-39 0.18428290363954858 1102 40-41 0.9000688062286031 1102 42-43 0.3913379322995283 1102 44-45 0.3240820663650723 1102 46-47 0.3570729433502038 1102 48-49 0.28817864959712836 1102 50-51 0.3013683493312911 1102 52-53 0.668713461290892 1102 54-55 0.37806854530343514 1102 56-57 0.2553368172643431 1102 58-59 0.3365765946142858 1102 60-61 0.33694964894026924 1102 62-63 0.34776581053095157 1102 64-65 0.8789907933342107 1102 66-67 0.48359116881560027 1102 68-69 0.3007360847543836 1102 70-71 0.3255101220948404 1102 72-73 0.3202428830835231 1102 74-75 0.5581450683202434 1102 76 0.8011011999779711 1103 1 0.1402801573937893 1103 2 0.005170624043714156 1103 3 0.04191058644903478 1103 4 0.07301415424865354 1103 5 0.016422316371908607 1103 6 0.058525037631042665 1103 7 0.15262304348761546 1103 8 0.09977815752004204 1103 9 0.10712086469388993 1103 10-11 0.08688146501170024 1103 12-13 0.06093610477462619 1103 14-15 0.11082016420763097 1103 16-17 0.058441163505264626 1103 18-19 0.599698676584552 1103 20-21 0.23468189327500966 1103 22-23 0.1736441508125992 1103 24-25 0.05360565571660203 1103 26-27 0.10121726447701462 1103 28-29 0.048833620763616636 1103 30-31 0.36769678654687965 1103 32-33 0.4357648091622366 1103 34-35 0.34966692662073484 1103 36-37 0.15216201712694755 1103 38-39 0.06240666370049297 1103 40-41 0.7173944535256709 1103 42-43 0.31409419332374 1103 44-45 0.19821279902209454 1103 46-47 0.29348061732489583 1103 48-49 0.3045975152845841 1103 50-51 0.1574917517162575 1103 52-53 0.6416852533779149 1103 54-55 0.28132213236078485 1103 56-57 0.09989804372798261 1103 58-59 0.17474723056322006 1103 60-61 0.16448738870961677 1103 62-63 0.13232206811397162 1103 64-65 0.6256711587476786 1103 66-67 0.4073430829817042 1103 68-69 0.22281633295857972 1103 70-71 0.16840729494254703 1103 72-73 0.17286210363143084 1103 74-75 0.4591673516382073 1103 76 0.7560984444130057 2116 1 0.09714822456989936 2116 2 0.0478128439644081 2116 3 0.039719173304305855 2116 4 0.06971328089257867 2116 5 0.09639347540773713 2116 6 0.027694067770269726 2116 7 0.16214712590108604 2116 8 0.09330488229930722 2116 9 0.08720007315113065 2116 10-11 0.10086360195210631 2116 12-13 0.07224055407684205 2116 14-15 0.12373014247271641 2116 16-17 0.12457449461579273 2116 18-19 0.6974776201800452 2116 20-21 0.27834771735762587 2116 22-23 0.18177645873574733 2116 24-25 0.11417402484760686 2116 26-27 0.12293848029869991 2116 28-29 0.07963781270606773 2116 30-31 0.45612466225566095 2116 32-33 0.5683014907425772 2116 34-35 0.3904335982192606 2116 36-37 0.16602057970496986 2116 38-39 0.07457457062586315 2116 40-41 0.787267315720463 2116 42-43 0.3028057899336858 2116 44-45 0.21937292888978988 2116 46-47 0.1528541911663055 2116 48-49 0.1235753490030902 2116 50-51 0.09564908709457143 2116 52-53 0.6634261098128462 2116 54-55 0.30120494510498474 2116 56-57 0.05039531071320624 2116 58-59 0.19052322754575357 2116 60-61 0.09396221850833086 2116 62-63 0.06290417004510829 2116 64-65 0.7223147498644948 2116 66-67 0.2863941027306751 2116 68-69 0.2409780653336 2116 70-71 0.2230666527309566 2116 72-73 0.13250965621305966 2116 74-75 0.4631490822210864 2116 76 0.6988818475217471 1117 1 0.26142506404790566 1117 2 0.07646634309290334 1117 3 0.06426367370551844 1117 4 0.06575394725022932 1117 5 0.011863151608721978 1117 6 0.05355573041093464 1117 7 -0.7116672716663146 1117 8 -0.05761915165571452 1117 9 0.0725413674191202 1117 10-11 0.090047490574797 1117 12-13 -0.017533809057951544 1117 14-15 -0.5989163538626912 1117 16-17 -0.1594896671528474 1117 18-19 0.03495262603264848 1117 20-21 0.12417145928193918 1117 22-23 0.1957494840873082 1117 24-25 0.16714695322057338 1117 26-27 0.2601969397259296 1117 28-29 0.1824438961837842 1117 30-31 0.4880101555612697 1117 32-33 0.4770010051717861 1117 34-35 0.3440774907315216 1117 36-37 0.16829461045343663 1117 38-39 0.07713079083818997 1117 40-41 0.8061792367360852 1117 42-43 0.33759124304009447 1117 44-45 0.22703588030299215 1117 46-47 0.20975095953711076 1117 48-49 0.20363430781794278 1117 50-51 0.14807580699171297 1117 52-53 0.7604299665368757 1117 54-55 0.3467957298443167 1117 56-57 0.2263054105125235 1117 58-59 0.14194469391425457 1117 60-61 0.27073937733789677 1117 62-63 0.09492686720754051 1117 64-65 0.7241808839483497 1117 66-67 0.45918846535450086 1117 68-69 0.2513661152765323 1117 70-71 0.1671193751964637 1117 72-73 0.36014328984982313 1117 74-75 0.4074087575304972 1117 76 0.6168663976844471 2117 1 0.14540024034356236 2117 2 0.07756084558907617 2117 3 0.0694552135706914 2117 4 0.0648851381673694 2117 5 0.10059419052090846 2117 6 0.008911783753568159 2117 7 0.1791632713528628 2117 8 0.12302911654971638 2117 9 0.11113620094773324 2117 10-11 0.13115374825763837 2117 12-13 0.09398946207214465 2117 14-15 0.0026857274121354635 2117 16-17 0.07656913654864894 2117 18-19 0.4999407590020155 2117 20-21 0.28890845754926175 2117 22-23 0.1815662805829632 2117 24-25 0.12079810597192164 2117 26-27 0.19294849159312832 2117 28-29 0.10590492961690501 2117 30-31 0.5209755222410806 2117 32-33 0.4525512453534972 2117 34-35 0.41540860364071364 2117 36-37 0.3204255337163957 2117 38-39 0.18447770537165553 2117 40-41 0.702188245446294 2117 42-43 0.2352735403511872 2117 44-45 0.19680189758043554 2117 46-47 0.17238548404726117 2117 48-49 0.27140738129274666 2117 50-51 0.21200641025236422 2117 52-53 0.8489898584673199 2117 54-55 0.36099936094228724 2117 56-57 0.160504377588488 2117 58-59 0.20549108100699698 2117 60-61 0.17726999803078058 2117 62-63 0.2010540782792205 2117 64-65 0.78386733002786 2117 66-67 0.42898501474955 2117 68-69 0.2402787142295253 2117 70-71 0.30473417274474457 2117 72-73 0.292143095986944 2117 74-75 0.3843935315812601 2117 76 0.6163551550516857 1116 1 0.23732806694886932 1116 2 0.06774332216027545 1116 3 0.05946215103220709 1116 4 0.07896621580006524 1116 5 0.06690442510228678 1116 6 -0.12049900176317863 1116 7 0.12318696210699898 1116 8 0.10262447710695 1116 9 0.09299823654088613 1116 10-11 -0.015304741230117713 1116 12-13 0.028451707266071935 1116 14-15 0.12716000752229917 1116 16-17 0.005963029695330135 1116 18-19 0.5768732738297615 1116 20-21 0.23498254048946876 1116 22-23 0.14782207906591083 1116 24-25 0.14513614455339763 1116 26-27 0.0837845244236064 1116 28-29 0.050673982097507064 1116 30-31 0.3774538128275893 1116 32-33 0.3780178419481075 1116 34-35 0.2339692414030594 1116 36-37 0.1068100013923754 1116 38-39 0.1281921129325454 1116 40-41 0.644333826701768 1116 42-43 0.09352583748787424 1116 44-45 0.07256692604618564 1116 46-47 0.20195487527783484 1116 48-49 0.1776025221158335 1116 50-51 0.16792952955823637 1116 52-53 0.7683225862599556 1116 54-55 0.26723288652247845 1116 56-57 -0.157963362394959 1116 58-59 0.02098963054010028 1116 60-61 -0.012832037217762604 1116 62-63 0.1596787460215836 1116 64-65 0.5489909499714827 1116 66-67 0.36167143287377 1116 68-69 0.18147349710256577 1116 70-71 0.18748794496379872 1116 72-73 0.14221605523069059 1116 74-75 0.42783410223362495 1116 76 0.5465004355881078 2118 1 0.03679439096590187 2118 2 0.05804744436427001 2118 3 0.10409519871403461 2118 4 0.07869340952597526 2118 5 0.11280774635862656 2118 6 -0.05909198223677947 2118 7 0.181765346102587 2118 8 0.07555366890648685 2118 9 0.0789147417128504 2118 10-11 0.07713910321950124 2118 12-13 0.05436396649027131 2118 14-15 0.14056437787468212 2118 16-17 0.105392242972421 2118 18-19 0.6424243259822262 2118 20-21 0.21313139813126725 2118 22-23 0.11079146693901976 2118 24-25 0.06438868840478307 2118 26-27 0.267569733160677 2118 28-29 0.06677285650832232 2118 30-31 0.3986488571291744 2118 32-33 0.44551808563576856 2118 34-35 0.3548968694712613 2118 36-37 0.2021477401030225 2118 38-39 0.161828858058378 2118 40-41 0.7872847273548587 2118 42-43 0.23720026333495525 2118 44-45 0.11874181448680332 2118 46-47 0.26432941743414773 2118 48-49 0.268048437497562 2118 50-51 0.31176844457815633 2118 52-53 0.7584160631955399 2118 54-55 0.2701308078254101 2118 56-57 0.06104627854985267 2118 58-59 0.06702615395146694 2118 60-61 0.22050168849401786 2118 62-63 0.28244611269138886 2118 64-65 0.7805602117878578 2118 66-67 0.4085295760894212 2118 68-69 0.16844849474829715 2118 70-71 0.2870843321083285 2118 72-73 0.15848984782027742 2118 74-75 0.4154505353731608 2118 76 0.47838580986757506 1119 1 0.11921652230108037 1119 2 0.09795152970313836 1119 3 0.06477016221508336 1119 4 0.08480323194014971 1119 5 0.1043336822323866 1119 6 -0.06818340002384815 1119 7 0.2239037521540368 1119 8 0.16882318630644022 1119 9 0.12690633296531217 1119 10-11 0.15174884424228452 1119 12-13 0.08947378127400185 1119 14-15 0.04011054459476071 1119 16-17 0.035475529251364435 1119 18-19 0.5462331884867382 1119 20-21 0.22594976562354674 1119 22-23 0.16185323028339127 1119 24-25 0.1593222654035813 1119 26-27 0.1607062265145629 1119 28-29 0.2047000086746067 1119 30-31 0.3660842399993456 1119 32-33 0.5033628926110154 1119 34-35 0.2614464088468793 1119 36-37 0.18322203009035576 1119 38-39 0.2510676196884418 1119 40-41 0.7964859980778698 1119 42-43 0.15073965676565848 1119 44-45 0.14092513840925136 1119 46-47 0.34699465466460566 1119 48-49 0.0949869259577909 1119 50-51 0.29083910756185816 1119 52-53 0.647303239474013 1119 54-55 0.3066523180425662 1119 56-57 0.08779409497157786 1119 58-59 0.2739919848303458 1119 60-61 0.2898663142716842 1119 62-63 0.3883907868969345 1119 64-65 0.8059088212668968 1119 66-67 0.32550907817761754 1119 68-69 0.25828276153908547 1119 70-71 0.3291334365487373 1119 72-73 0.14400771322944195 1119 74-75 0.4793686022260104 1119 76 0.6520463620063879 2119 1 0.13777440894528326 2119 2 0.08906326079209492 2119 3 0.07749581907249592 2119 4 0.09662104766281487 2119 5 0.10259538498050347 2119 6 0.046009491916116474 2119 7 0.11173198518771699 2119 8 0.15983627358991725 2119 9 0.08467711425100788 2119 10-11 0.1179601091275444 2119 12-13 0.11494446017919557 2119 14-15 0.1718876605952815 2119 16-17 0.11738350546272613 2119 18-19 0.6596048271933839 2119 20-21 0.3177715957728964 2119 22-23 0.19261077027301354 2119 24-25 0.11803603299352261 2119 26-27 0.2103688253130116 2119 28-29 0.08303067624346738 2119 30-31 0.42691433002097057 2119 32-33 0.4011789182528531 2119 34-35 0.4024299560094562 2119 36-37 0.1701725301894612 2119 38-39 0.17034537635611713 2119 40-41 0.6740219858982428 2119 42-43 0.25847506565015976 2119 44-45 0.2145274299147033 2119 46-47 0.2781325678949145 2119 48-49 0.1442629910117077 2119 50-51 0.2701823953268203 2119 52-53 0.664555503269824 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-0.09716598167641166 2111 4 -0.09291359758372408 2111 5 -0.04406430907716441 2111 6 0.03390772431768596 2111 7 -0.11042784734733857 2111 8 -0.01913075005980147 2111 9 -0.06161129984550939 2111 10-11 -0.06859602983609392 2111 12-13 -0.005359565068985717 2111 14-15 -0.02685843093053819 2111 16-17 0.02412674754624078 2111 18-19 0.5114153354579756 2111 20-21 0.09174560158837153 2111 22-23 -0.07040060933833558 2111 24-25 -0.005890217713435675 2111 26-27 -0.10576143976654606 2111 28-29 -0.015378463187879277 2111 30-31 -12.49401419576727 2111 32-33 -10.753217614670845 2111 34-35 -5.539707949505193 2111 36-37 -1.340133833596859 2111 38-39 -0.2888754689924511 2111 40-41 -6.509161693418317 2111 42-43 -0.6736632001541807 2111 44-45 -0.06412558741111951 2111 46-47 -0.0749144173213594 2111 48-49 -0.08514936789952543 2111 50-51 -0.0753979921843353 2111 52-53 0.2011629906821355 2111 54-55 -0.00771561387200137 2111 56-57 -0.03695138890472549 2111 58-59 0.030062702939545716 2111 60-61 -0.03306601424335298 2111 62-63 -0.15760059598189002 2111 64-65 0.3784118785353954 2111 66-67 -0.11974069533614795 2111 68-69 -0.0652961822190008 2111 70-71 -0.10026261987058405 2111 72-73 0.05282938163467321 2111 74-75 0.19970784667265917 2111 76 0.21560793196402273 2110 1 0.10129062079907669 2110 2 -0.031706209605651736 2110 3 0.0012416215209825054 2110 4 0.027657776573320803 2110 5 -0.03368001241946672 2110 6 0.047782996391205756 2110 7 -0.05302494260636337 2110 8 -0.18842279350771207 2110 9 -0.04620303122665348 2110 10-11 -0.07382599730586747 2110 12-13 -0.051829340357997467 2110 14-15 0.016355023054522633 2110 16-17 -0.09535019219371321 2110 18-19 0.5370326846324431 2110 20-21 0.06508573881389168 2110 22-23 -0.05557841526320573 2110 24-25 -0.06427434372974972 2110 26-27 -0.20144975124028974 2110 28-29 -0.12211856916319874 2110 30-31 0.042448064105613525 2110 32-33 -0.26194305454764333 2110 34-35 -0.1463187086888098 2110 36-37 -0.05138623610053372 2110 38-39 -0.06844988173869382 2110 40-41 0.1705109713685431 2110 42-43 -0.27170550331720733 2110 44-45 -0.2944702270464532 2110 46-47 -0.4030499283443376 2110 48-49 -0.22817864955258926 2110 50-51 -0.34739499694389764 2110 52-53 0.2803769912348315 2110 54-55 -0.08739739034031402 2110 56-57 -0.030693098340556446 2110 58-59 -0.12628781036590908 2110 60-61 -0.3338830586972463 2110 62-63 -0.1646179120407396 2110 64-65 0.47242739810732104 2110 66-67 -0.014172416441503799 2110 68-69 -0.2533266328677115 2110 70-71 -0.1826603680616401 2110 72-73 -0.2556960054241344 2110 74-75 -0.06474876977605248 2110 76 -0.6340114826729533 2109 1 -0.07182216681831477 2109 2 -0.018559164792876004 2109 3 0.004971997034353137 2109 4 -0.0502596049799493 2109 5 -0.09456798762234087 2109 6 0.041081403311160614 2109 7 -0.03195873979748143 2109 8 -0.10836891513500291 2109 9 -0.056550943098855555 2109 10-11 -0.05418093773193533 2109 12-13 -0.02998894425978449 2109 14-15 -0.0391551752099204 2109 16-17 -0.05588472322428828 2109 18-19 0.37831121295413084 2109 20-21 0.14426133476060699 2109 22-23 -0.09546703591579586 2109 24-25 -0.06968076834600367 2109 26-27 -0.10166056814453839 2109 28-29 -0.046411140296370945 2109 30-31 0.19155900653456825 2109 32-33 0.054812289385978374 2109 34-35 0.12000220540001294 2109 36-37 -0.06385006082343381 2109 38-39 -0.266675045029352 2109 40-41 0.25676445211103527 2109 42-43 0.03823642562260687 2109 44-45 -0.007949765831419597 2109 46-47 -0.27706188681387545 2109 48-49 -0.039336554934990886 2109 50-51 -0.13969287957936416 2109 52-53 0.23524401438437081 2109 54-55 -0.12135478342237604 2109 56-57 0.013145173350885386 2109 58-59 -0.06210399539271094 2109 60-61 -0.20545623605190144 2109 62-63 0.013605546288395942 2109 64-65 0.5721820208739956 2109 66-67 -0.1004832587260367 2109 68-69 -0.11447390871512653 2109 70-71 -0.23348788993535408 2109 72-73 -0.24153762146997337 2109 74-75 0.02611944702304214 2109 76 -0.7483247847086574 2108 1 -0.08122595843742886 2108 2 -0.051474215689310654 2108 3 -2.195593503984128E-5 2108 4 0.019100017485776277 2108 5 0.03427500825142005 2108 6 0.0032060800612825346 2108 7 0.0505762840624584 2108 8 0.035364009035973254 2108 9 0.009626425084107382 2108 10-11 -0.057579218998817794 2108 12-13 0.03517945395469724 2108 14-15 0.040837791445959226 2108 16-17 -0.036961414008892746 2108 18-19 0.5790395125974115 2108 20-21 0.12221339098985595 2108 22-23 -0.0575776256321987 2108 24-25 -0.09437057274809746 2108 26-27 -0.11874701219862516 2108 28-29 -0.00899464080173118 2108 30-31 0.37125156111485325 2108 32-33 0.17271579687603378 2108 34-35 0.16719141314878527 2108 36-37 0.01937529403270588 2108 38-39 0.08925577750657254 2108 40-41 0.5380904435860998 2108 42-43 0.04658590117804806 2108 44-45 -0.019637536658507315 2108 46-47 0.01884265864951118 2108 48-49 0.032157507457696966 2108 50-51 0.08079388913551355 2108 52-53 0.39808476137487503 2108 54-55 0.021119109345619336 2108 56-57 -0.0809980749306689 2108 58-59 0.019495047909721563 2108 60-61 -0.17545936837129972 2108 62-63 -0.09678080376956899 2108 64-65 0.38988157376878974 2108 66-67 0.03637909622617741 2108 68-69 0.04626863320672925 2108 70-71 -0.10266203902101978 2108 72-73 -0.09310244765499931 2108 74-75 0.11575151625924462 2108 76 -0.6584555689950804 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 2.0 12 26.0 13 237.0 14 659.0 15 1152.0 16 1711.0 17 2973.0 18 4765.0 19 7236.0 20 8434.0 21 7988.0 22 7596.0 23 7442.0 24 7660.0 25 7931.0 26 8780.0 27 9708.0 28 11412.0 29 13770.0 30 17663.0 31 26799.0 32 42256.0 33 57982.0 34 89786.0 35 149986.0 36 363426.0 37 1602594.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.92525042947609 2.083274050863952 51.63680591746092 3.354669602199048 2 2.7369801469446426 2.188234509795632 3.756665720857212 91.31811962240252 3 2.8784044059002247 48.91759018591254 4.153580484996996 44.05042492319025 4 2.9202341162955383 1.7907099831136426 92.13251847377248 3.1565374268183324 5 2.8965753296579555 2.1609984495771095 50.896228984533984 44.04619723623095 6 50.084309834168984 1.8290030707641625 4.436063145382837 43.65062394968402 7 2.766573955659694 2.2579100429516736 51.64452957632886 43.33098642505978 8 3.5947127896473705 2.3133171326201007 91.9464189458913 2.1455511318412306 9 4.0785796923056905 2.352707792846591 91.08372690117864 2.4849856136690875 10-11 70.78454894238719 23.438011946467725 2.4848230103244995 3.292616100820578 12-13 23.08172769305692 26.44219003940692 23.583867146563335 26.892215120972825 14-15 2.9180389711435972 46.46429189902007 26.968049255805145 23.64961987403119 16-17 26.1504796392157 2.8494203597273793 46.91858531838141 24.081514682675508 18-19 23.349779306610557 46.4467713886407 3.2550750536387785 26.948374251109968 20-21 26.52816655785793 2.0166270050008657 44.8179330350646 26.637273402076605 22-23 23.01857661910248 2.41291168118037 49.92575124777742 24.642760451939736 24-25 3.4195686621078107 26.503674429079332 23.206586736282578 46.87017017253028 26-27 21.078373186058062 28.789958755661644 20.589953389751273 29.541714668529018 28-29 21.103515728215015 8.087321248110753 23.734763050341183 47.07439997333305 30-31 47.4917621080548 9.793497817456608 26.240643193789854 16.47409688069874 32-33 58.64736781770864 14.297671438803825 3.835406390474046 23.21955435301349 34-35 3.1612325983933163 46.120731357323294 27.30622356171244 23.411812482570955 36-37 21.64047262288138 11.8094134328249 39.306756900682686 27.243357043611027 38-39 47.063851081352894 2.851229321935923 47.732435383463404 2.3524842132477826 40-41 23.388621180549062 39.92845046329758 28.284831465698417 8.398096890454939 42-43 34.991182833639705 2.211507113489817 54.02103843373954 8.776271619130933 44-45 2.9913324287167264 3.611765815411057 84.9138852687061 8.483016487166124 46-47 9.434469632605873 77.98726734510201 9.250646551548918 3.3276164707431866 48-49 15.4639845786988 23.576407718130355 37.7979807916669 23.161626911503944 50-51 44.152641450682 17.062538059345343 29.490718194582545 9.294102295390113 52-53 45.52131445291698 24.461356095633533 26.5357885896355 3.4815408618139863 54-55 14.98613399979024 55.29869421384128 2.7217767342256463 26.993395052142827 56-57 22.231413827951027 26.70772130111944 9.333188074345502 41.72767679658403 58-59 1.9794111645082428 26.953110073521103 44.42085566758023 26.646623094390424 60-61 8.313543151269078 40.833236448840516 27.86496930455363 22.988251095336782 62-63 2.2424830506338687 17.575714214865684 59.63743925748809 20.544363477012357 64-65 29.267179246609924 44.00052602181974 23.693014641618163 3.03928008995217 66-67 22.627474924531725 23.798056402222137 26.50747528225908 27.066993390987058 68-69 2.6553329425432954 27.046810250840046 46.56421165426951 23.733645152347137 70-71 43.37783651371925 4.374964938653823 26.12887371980354 26.118324827823386 72-73 35.34602398236729 59.12865339227162 2.6939918877191387 2.8313307376419425 74-75 19.748379454416998 33.66391270802049 22.761805612579643 23.825902224982865 76 10.587225718645806 81.94806123967165 2.3296994195873615 5.135013622095193 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2.0 1 2.5 2 3.0 3 3.0 4 3.0 5 3.5 6 5.0 7 8.0 8 10.0 9 10.5 10 12.5 11 27.0 12 40.0 13 37.5 14 39.0 15 54.5 16 85.5 17 105.0 18 118.0 19 168.0 20 219.0 21 233.0 22 270.5 23 363.5 24 494.5 25 570.0 26 650.0 27 828.0 28 1158.5 29 1391.0 30 1975.0 31 3967.5 32 18036.0 33 30696.0 34 119433.0 35 143429.0 36 307642.5 37 536597.0 38 308050.5 39 54004.0 40 32526.0 41 143644.5 42 250741.0 43 142368.0 44 25741.0 45 17840.5 46 18194.0 47 36131.5 48 228621.5 49 236857.5 50 70541.0 51 129674.5 52 120950.0 53 149088.0 54 245084.0 55 140021.0 56 22126.0 57 7060.5 58 4827.0 59 4104.0 60 3209.0 61 3059.0 62 3081.0 63 3124.0 64 3255.5 65 3471.5 66 3702.5 67 3806.0 68 3897.0 69 3995.0 70 3943.0 71 3884.0 72 3873.5 73 3725.5 74 3507.5 75 3427.0 76 3284.0 77 2922.0 78 2493.5 79 2284.0 80 2172.5 81 1888.5 82 1554.0 83 1392.0 84 1240.0 85 995.0 86 784.0 87 666.0 88 576.0 89 427.5 90 309.5 91 211.0 92 172.0 93 138.5 94 94.0 95 68.5 96 54.0 97 49.5 98 37.5 99 63.0 100 96.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 2459974.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 8.026116272603877 #Duplication Level Percentage of deduplicated Percentage of total 1 90.2578222435664 7.244197758388764 2 3.899657778594856 0.6259821350873291 3 2.08222279629444 0.5013648680557665 4 0.9664676475346097 0.3102792685129087 5 0.6938894535278943 0.2784618717174222 6 0.40806399505768426 0.19651014425977364 7 0.24114956013521516 0.13548460861127623 8 0.14514150472316573 0.09319380743110503 9 0.07318701154358098 0.05286667178638648 >10 0.8168164381922934 1.3897637570847516 >50 0.16354015880371991 0.9549722069383174 >100 0.17504905978092247 3.0990989072643447 >500 0.038237813237360266 2.0749004961989748 >1k 0.0304869040194566 5.479802509652441 >5k 0.00465054468093402 2.6006967113599893 >10k+ 0.0036170903073931265 74.96242427765057 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TCATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTG 503942 20.48566366961602 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAA 413363 16.803551582252496 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCAC 247582 10.064415314958614 No Hit TCATTGTTTGGCATAGTCACGTTCAGCACTGGATATGTGCTTCCTGATTT 245180 9.966772006533402 No Hit TCATTGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTG 204567 8.315819598093313 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAA 179634 7.302272300438947 No Hit TCATTGTTTGGCATAGTCACGTTCAATGCTAGGTATGTGTAGTTTAAGTG 13226 0.5376479588808663 No Hit ATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGG 9878 0.40154895946054714 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCATCCAGCACACTGGAGTTTAACAA 7834 0.3184586503759796 No Hit GCCTCCCTTAGGTCACTAATTGCCTCATCCGGCACACTGGAGTTTAACAA 7754 0.31520658348421565 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGTAGTTTAACAA 7595 0.3087431005368349 No Hit TGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTCAACC 6905 0.2806940235953713 No Hit TCATTGTTTGGCATAGTCGCGTTCAATGCTGGGTATGTGTAGTTTAAGTG 6366 0.2587832229121121 No Hit CATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGG 5846 0.23764478811564677 No Hit TCATTGTTTGGCATAGTCACGTTCAGTGCTAGATATTTGTATTTTAATTG 5453 0.2216690095098566 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGTAGTTTATCAC 5077 0.2063842951185663 No Hit GCCTCCCTTAGGTCACTAATTGCCTCGTCAGGCACTCTGGAGTTTATCAC 4989 0.202807021537626 No Hit TCATTGTTTGGCATAGTCACGTTCAGCACTAGATATGTGCTTCCTGATTT 4812 0.19561182353959838 No Hit TCATTGTTTGGCATAGTCACTCAAAACGGAACAAGTTCTGCTTGCATAAG 4641 0.18866053055845305 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCGTCAAGCACTCTGGAGTTTATCAC 4381 0.1780913131602204 No Hit TTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGT 4176 0.1697578917500754 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTAACAC 4131 0.16792860412345822 No Hit TGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTC 4045 0.16443263221481202 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCATCCAGCACCCTGGAGTTTAACAA 3613 0.146871470999287 No Hit TCATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTATTTTAAGTG 3510 0.14268443487614096 No Hit TCATTGTTTGGCATAGTCACGTTCAGCACTGGATATGTGTTTCCTGATTT 3485 0.14166816397246476 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGTAGTTTAACAA 3223 0.13101764490193799 No Hit TCATTGTTTGGCATAGTCGCGTTCAGTGCTGGATATTTGTATTTTAATTG 3097 0.12589563954740984 No Hit GCCTCCCTTAGGTCACTAATTGCCTCATCCGGCACCCTGGAGTTTAACAA 3067 0.12467611446299838 No Hit TCATTGTTTGGACTGGAGTCACTCAAAACGGAACAAGTTCTGCTTGCATA 2801 0.11386299204788344 No Hit CTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAATG 2749 0.11174914856823691 No Hit TCATTGTTTGGCATAGTCACGTTCAGTGCTGGGTATGTGTAGTTTAAGTG 2721 0.11061092515611953 No Hit TTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTCAAC 2711 0.11020441679464904 No Hit GTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTCAACCAATTTAA 2590 0.10528566562085614 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.001951240135058338 0.0 0.0 2 0.001951240135058338 0.0 0.0 3 0.001951240135058338 0.0 0.0 4 0.001951240135058338 0.0 0.0 5 0.001951240135058338 0.0 0.0 6 0.001951240135058338 0.0 0.0 7 0.001951240135058338 0.0 0.0 8 0.001951240135058338 0.0 0.0 9 0.001951240135058338 0.0 0.0 10 0.001951240135058338 0.0 0.0 11 0.0019918909712053866 0.0 0.0 12 0.0019918909712053866 0.0 0.0 13 0.0019918909712053866 0.0 0.0 14 0.0020325418073524355 0.0 0.0 15 0.0020325418073524355 0.0 0.0 16 0.0020325418073524355 0.0 0.0 17 0.0020325418073524355 0.0 0.0 18 0.0020325418073524355 0.0 0.0 19 0.0020325418073524355 0.0 0.0 20 0.0020731926434994843 0.0 0.0 21 0.0020731926434994843 0.0 0.0 22 0.0020731926434994843 0.0 0.0 23 0.0020731926434994843 0.0 0.0 24 0.0020731926434994843 0.0 0.0 25 0.0020731926434994843 0.0 0.0 26 0.0020731926434994843 0.0 0.0 27 0.0020731926434994843 0.0 0.0 28 0.0020731926434994843 0.0 0.0 29 0.0020731926434994843 0.0 0.0 30 0.0021544943157935817 0.0 0.0 31 0.0021544943157935817 0.0 0.0 32 0.0021544943157935817 0.0 0.0 33 0.00219514515194063 0.0 0.0 34 0.002235795988087679 0.0 0.0 35 0.0023577484965288252 0.0 0.0 36 0.0024390501688229226 0.0 0.0 37 0.0026016535134111172 0.0 0.0 38 0.0028049076941463608 0.0 0.0 39 0.002967511038734556 0.0 0.0 40 0.0030894635471757016 0.0 0.0 41 0.003292717727910945 0.0 0.0 42 0.003374019400205043 0.0 0.0 43 0.0034959719086461887 0.0 0.0 44 0.0036992260893814323 0.0 0.0 45 0.003943131106263725 0.0 0.0 46 0.003983781942410773 0.0 0.0 47 0.004308988631587163 0.0 0.0 48 0.004552893648469456 0.0 0.0 49 0.004959402009939943 0.0 0.0 50 0.0052033070268222344 0.0 0.0 51 0.005528513715998625 0.0 0.0 52 0.005772418732880917 0.0 0.0 53 0.005975672913616161 0.0 0.0 54 0.006544784619674842 0.0 0.0 55 0.006788689636557134 0.0 0.0 56 0.007317150506468768 0.0 0.0 57 0.007479753851056962 0.0 0.0 58 0.00788626221252745 0.0 0.0 59 0.008292770573997937 0.0 0.0 60 0.008658628099321376 0.0 0.0 61 0.009065136460791863 0.0 0.0 62 0.009837502347585788 0.0 0.0 63 0.010162709036762177 0.0 0.0 64 0.010406614053644469 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTAGATA 1490 0.0 70.00001 29 TTAGGAT 15 0.0022252065 70.00001 8 ATTCGTG 30 1.02478225E-7 70.00001 47 ATTAACG 15 0.0022252065 70.00001 50 ATATGTC 50 0.0 70.00001 33 AAACGAC 15 0.0022252065 70.00001 61 CCGTAGG 15 0.0022252065 70.00001 6 TGCTAGT 15 0.0022252065 70.00001 27 TATTGGA 35 3.7161954E-9 70.0 61 AGGTTAC 130 0.0 70.0 10 TTGTTAG 35 3.7161954E-9 70.0 4 CGCTAGT 20 7.9304125E-5 69.99999 14 ACGTACT 20 7.9304125E-5 69.99999 11 TCATTGT 115865 0.0 69.885216 1 GCCTCCC 99295 0.0 69.82023 1 TTAGACT 2230 0.0 69.52915 63 GGGACAA 965 0.0 69.274605 50 CCTCCCT 101310 0.0 69.2296 2 GCTAGGT 1705 0.0 69.17889 28 ATGCTAG 1695 0.0 69.17404 26 >>END_MODULE