FastQCFastQC Report
Tue 25 Aug 2015
000000000-AHV5L l01n02 kokuya4.34200000033d04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AHV5L l01n02 kokuya4.34200000033d04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2459974
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTG50394220.48566366961602No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAA41336316.803551582252496No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCAC24758210.064415314958614No Hit
TCATTGTTTGGCATAGTCACGTTCAGCACTGGATATGTGCTTCCTGATTT2451809.966772006533402No Hit
TCATTGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTG2045678.315819598093313No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAA1796347.302272300438947No Hit
TCATTGTTTGGCATAGTCACGTTCAATGCTAGGTATGTGTAGTTTAAGTG132260.5376479588808663No Hit
ATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGG98780.40154895946054714No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCATCCAGCACACTGGAGTTTAACAA78340.3184586503759796No Hit
GCCTCCCTTAGGTCACTAATTGCCTCATCCGGCACACTGGAGTTTAACAA77540.31520658348421565No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGTAGTTTAACAA75950.3087431005368349No Hit
TGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTCAACC69050.2806940235953713No Hit
TCATTGTTTGGCATAGTCGCGTTCAATGCTGGGTATGTGTAGTTTAAGTG63660.2587832229121121No Hit
CATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGG58460.23764478811564677No Hit
TCATTGTTTGGCATAGTCACGTTCAGTGCTAGATATTTGTATTTTAATTG54530.2216690095098566No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGTAGTTTATCAC50770.2063842951185663No Hit
GCCTCCCTTAGGTCACTAATTGCCTCGTCAGGCACTCTGGAGTTTATCAC49890.202807021537626No Hit
TCATTGTTTGGCATAGTCACGTTCAGCACTAGATATGTGCTTCCTGATTT48120.19561182353959838No Hit
TCATTGTTTGGCATAGTCACTCAAAACGGAACAAGTTCTGCTTGCATAAG46410.18866053055845305No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCGTCAAGCACTCTGGAGTTTATCAC43810.1780913131602204No Hit
TTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGT41760.1697578917500754No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTAACAC41310.16792860412345822No Hit
TGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTC40450.16443263221481202No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCATCCAGCACCCTGGAGTTTAACAA36130.146871470999287No Hit
TCATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTATTTTAAGTG35100.14268443487614096No Hit
TCATTGTTTGGCATAGTCACGTTCAGCACTGGATATGTGTTTCCTGATTT34850.14166816397246476No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGTAGTTTAACAA32230.13101764490193799No Hit
TCATTGTTTGGCATAGTCGCGTTCAGTGCTGGATATTTGTATTTTAATTG30970.12589563954740984No Hit
GCCTCCCTTAGGTCACTAATTGCCTCATCCGGCACCCTGGAGTTTAACAA30670.12467611446299838No Hit
TCATTGTTTGGACTGGAGTCACTCAAAACGGAACAAGTTCTGCTTGCATA28010.11386299204788344No Hit
CTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAATG27490.11174914856823691No Hit
TCATTGTTTGGCATAGTCACGTTCAGTGCTGGGTATGTGTAGTTTAAGTG27210.11061092515611953No Hit
TTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTCAAC27110.11020441679464904No Hit
GTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTCAACCAATTTAA25900.10528566562085614No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGATA14900.070.0000129
TTAGGAT150.002225206570.000018
ATTCGTG301.02478225E-770.0000147
ATTAACG150.002225206570.0000150
ATATGTC500.070.0000133
AAACGAC150.002225206570.0000161
CCGTAGG150.002225206570.000016
TGCTAGT150.002225206570.0000127
TATTGGA353.7161954E-970.061
AGGTTAC1300.070.010
TTGTTAG353.7161954E-970.04
CGCTAGT207.9304125E-569.9999914
ACGTACT207.9304125E-569.9999911
TCATTGT1158650.069.8852161
GCCTCCC992950.069.820231
TTAGACT22300.069.5291563
GGGACAA9650.069.27460550
CCTCCCT1013100.069.22962
GCTAGGT17050.069.1788928
ATGCTAG16950.069.1740426
Produced by FastQC (version 0.11.2)