##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AHV5L l01n02 kokuya3.34200000033cf6.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1899566 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.786157469653595 34.0 34.0 34.0 32.0 34.0 2 33.30123038630929 34.0 34.0 34.0 34.0 34.0 3 33.196833908376966 34.0 34.0 34.0 34.0 34.0 4 33.30543818956541 34.0 34.0 34.0 34.0 34.0 5 33.26723525268403 34.0 34.0 34.0 34.0 34.0 6 36.87764205086846 38.0 38.0 38.0 36.0 38.0 7 36.98765612776813 38.0 38.0 38.0 36.0 38.0 8 37.11484939191373 38.0 38.0 38.0 37.0 38.0 9 37.19954452753945 38.0 38.0 38.0 37.0 38.0 10-11 37.01369181170857 38.0 38.0 38.0 36.5 38.0 12-13 36.9791994592449 38.0 38.0 38.0 36.5 38.0 14-15 36.88114864132123 38.0 38.0 38.0 36.5 38.0 16-17 37.04902303999966 38.0 38.0 38.0 37.0 38.0 18-19 36.262169358685085 38.0 38.0 38.0 33.5 38.0 20-21 36.76907093515045 38.0 38.0 38.0 35.5 38.0 22-23 36.804258972839065 38.0 37.5 38.0 36.0 38.0 24-25 36.97314018044122 38.0 38.0 38.0 36.5 38.0 26-27 36.494516379004466 38.0 38.0 38.0 35.0 38.0 28-29 36.807673963421124 38.0 38.0 38.0 36.5 38.0 30-31 36.352098584624066 38.0 38.0 38.0 34.5 38.0 32-33 36.126337542364936 38.0 37.0 38.0 33.0 38.0 34-35 36.29644639880899 38.0 37.0 38.0 34.0 38.0 36-37 36.32726185876142 38.0 37.0 38.0 34.5 38.0 38-39 36.79968740228031 38.0 38.0 38.0 36.0 38.0 40-41 35.58597305910929 38.0 37.0 38.0 29.5 38.0 42-43 36.25953533596622 38.0 37.0 38.0 34.0 38.0 44-45 36.395239754765036 38.0 37.0 38.0 34.0 38.0 46-47 35.949028357003655 38.0 37.0 38.0 33.0 38.0 48-49 36.386460380950176 38.0 37.0 38.0 34.0 38.0 50-51 36.30545029759429 38.0 37.5 38.0 34.0 38.0 52-53 35.741736007066876 38.0 37.0 38.0 30.5 38.0 54-55 36.07470864397446 38.0 37.0 38.0 34.0 38.0 56-57 36.362802871813884 38.0 37.0 38.0 34.0 38.0 58-59 36.398460227230856 38.0 37.5 38.0 34.0 38.0 60-61 36.43727830462326 38.0 37.5 38.0 34.0 38.0 62-63 36.26234676763008 38.0 37.0 38.0 34.0 38.0 64-65 35.75271298812466 38.0 37.0 38.0 30.5 38.0 66-67 36.288794650988706 38.0 37.0 38.0 34.0 38.0 68-69 36.490120111646554 38.0 38.0 38.0 34.5 38.0 70-71 36.46981363111363 38.0 38.0 38.0 34.5 38.0 72-73 36.00064672667335 38.0 37.0 38.0 33.0 38.0 74-75 35.99266885172719 38.0 37.0 38.0 33.0 38.0 76 33.501159212156885 37.0 33.0 38.0 23.0 38.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.19476737979059067 1101 2 0.0719387685605355 1101 3 0.09147787925360262 1101 4 0.12671637580039175 1101 5 0.047560472925738395 1101 6 0.0955772871094922 1101 7 0.14345490070328282 1101 8 0.11651049233281441 1101 9 0.15698241586029127 1101 10-11 0.11953681381595516 1101 12-13 0.1057233912270874 1101 14-15 0.16794817093643388 1101 16-17 0.17132252401216164 1101 18-19 0.7558668623907607 1101 20-21 0.26707635853989586 1101 22-23 0.20371721184907443 1101 24-25 0.1605379885350544 1101 26-27 0.20803855309866748 1101 28-29 0.17880835233107462 1101 30-31 0.5580168249259785 1101 32-33 0.5197654827637521 1101 34-35 0.4554407734514143 1101 36-37 0.28478830976866476 1101 38-39 0.16385024857386554 1101 40-41 0.847248207019824 1101 42-43 0.3170072736211864 1101 44-45 0.1551469070353022 1101 46-47 0.22721318292493464 1101 48-49 0.18113738996837014 1101 50-51 0.2045533279304408 1101 52-53 0.7493955952711246 1101 54-55 0.38421242429858893 1101 56-57 0.18254135538444416 1101 58-59 0.17510933587799116 1101 60-61 0.23704028732533544 1101 62-63 0.33060931542804894 1101 64-65 0.7189950871675208 1101 66-67 0.29308802138240964 1101 68-69 0.2388289998324069 1101 70-71 0.2310263883559358 1101 72-73 0.2107776615265422 1101 74-75 0.5464509923326304 1101 76 0.4833942853331763 1102 1 0.04157573949250093 1102 2 0.05030692562944239 1102 3 0.1447134539515602 1102 4 0.10158950038934478 1102 5 0.06754139179307117 1102 6 0.04242748392440632 1102 7 0.09702986822249215 1102 8 0.03276323729278374 1102 9 0.0073818988748968195 1102 10-11 0.07985950939276165 1102 12-13 0.10003805177201741 1102 14-15 0.09996756240071392 1102 16-17 0.1308729208547703 1102 18-19 0.7183836667973651 1102 20-21 0.26608254954691546 1102 22-23 0.16819493770159966 1102 24-25 0.12627373139197573 1102 26-27 0.1770074600270064 1102 28-29 0.18480061843951034 1102 30-31 0.4669033093158532 1102 32-33 0.5759509624696619 1102 34-35 0.4401109851177978 1102 36-37 0.1997728437402415 1102 38-39 0.156067810251443 1102 40-41 0.9033932241294238 1102 42-43 0.31784359809503826 1102 44-45 0.24966358393355392 1102 46-47 0.35503972610401746 1102 48-49 0.23621189253329078 1102 50-51 0.3034199950483156 1102 52-53 0.5993581992257404 1102 54-55 0.3743361458413901 1102 56-57 0.20178423398837708 1102 58-59 0.2677492752555324 1102 60-61 0.32480192490697846 1102 62-63 0.28630045375597746 1102 64-65 0.8391093174427695 1102 66-67 0.4202873838740473 1102 68-69 0.19599013989749636 1102 70-71 0.23976388599857756 1102 72-73 0.31226384830221576 1102 74-75 0.5641151612225457 1102 76 0.8205054506450864 1103 1 0.11574880374440966 1103 2 0.0751423348969169 1103 3 0.08556783279872349 1103 4 0.0693257731419692 1103 5 -0.0022970891860012443 1103 6 -0.04614626109766817 1103 7 0.09146570402126031 1103 8 0.055326297009472114 1103 9 0.039067391458871725 1103 10-11 0.06000766089843523 1103 12-13 0.010234469738882979 1103 14-15 0.05794638129111007 1103 16-17 0.06452151756015923 1103 18-19 0.5972466418275175 1103 20-21 0.17714305445486644 1103 22-23 0.1089333111003512 1103 24-25 0.011255929805329856 1103 26-27 0.02862471170172398 1103 28-29 0.06421020192361482 1103 30-31 0.396937107106325 1103 32-33 0.34163551369375256 1103 34-35 0.2858135594400295 1103 36-37 -0.0023965477740475194 1103 38-39 0.06469002404000435 1103 40-41 0.7787074055522183 1103 42-43 0.2631572084534781 1103 44-45 0.20340871612214073 1103 46-47 0.2111735077868886 1103 48-49 0.21323116198577452 1103 50-51 0.17669351771380093 1103 52-53 0.5648309572796038 1103 54-55 0.34214226514160373 1103 56-57 0.08754492371119227 1103 58-59 0.15688788742575355 1103 60-61 0.13111027814433385 1103 62-63 0.016836122358370176 1103 64-65 0.6072390740182669 1103 66-67 0.3938430712268186 1103 68-69 0.20208881264664313 1103 70-71 0.2181615204093248 1103 72-73 0.2646059675757968 1103 74-75 0.43180038389539277 1103 76 0.6767639445870657 2116 1 -0.003222385990419241 2116 2 0.05432771527809166 2116 3 0.04801757811382856 2116 4 0.08135313319778703 2116 5 0.1511762654150246 2116 6 0.0781274556607201 2116 7 0.21771011029291287 2116 8 0.12799998002902413 2116 9 0.15304337317874683 2116 10-11 0.20205676262740013 2116 12-13 0.15323209400494875 2116 14-15 0.20683154718604158 2116 16-17 0.1895107830549847 2116 18-19 0.7470530002730982 2116 20-21 0.36050426564851534 2116 22-23 0.2517933298401758 2116 24-25 0.14590058009745377 2116 26-27 0.1418975037000081 2116 28-29 0.1673057274251022 2116 30-31 0.5814013285227873 2116 32-33 0.572553576992803 2116 34-35 0.38528853065116664 2116 36-37 0.2196134688889586 2116 38-39 0.131295494471253 2116 40-41 0.8050423639048816 2116 42-43 0.2038927380219775 2116 44-45 0.24924543544331357 2116 46-47 0.2824209350094904 2116 48-49 0.1365429651507526 2116 50-51 0.18301954954502264 2116 52-53 0.6826719979239471 2116 54-55 0.4140043940876623 2116 56-57 0.2422144907965631 2116 58-59 0.2214092074908649 2116 60-61 0.16053142266046905 2116 62-63 0.1344313205370895 2116 64-65 0.7657883524683982 2116 66-67 0.337800099059578 2116 68-69 0.3743740531033737 2116 70-71 0.287893503067977 2116 72-73 0.20853184304636585 2116 74-75 0.5661401360168696 2116 76 0.9835417047819206 1117 1 0.2771378623993712 1117 2 0.07773289408504525 1117 3 0.05693223204419695 1117 4 0.03877921731904621 1117 5 0.07505392936779032 1117 6 0.03389103913596614 1117 7 -0.7252806177375746 1117 8 0.012220404748845226 1117 9 0.07853122617179054 1117 10-11 0.12109621230089829 1117 12-13 -0.02913960915588376 1117 14-15 -0.5627488128942773 1117 16-17 -0.1921854071054696 1117 18-19 -0.01782227751413501 1117 20-21 0.07618327239188005 1117 22-23 0.12664648969226278 1117 24-25 0.14911694552070287 1117 26-27 0.18073712724132918 1117 28-29 0.1834660639673018 1117 30-31 0.4292609645096448 1117 32-33 0.4170211099732697 1117 34-35 0.34335002220841204 1117 36-37 0.19067571801909367 1117 38-39 0.1005798212839153 1117 40-41 0.8934258680597651 1117 42-43 0.3550001263955238 1117 44-45 0.19626861095525783 1117 46-47 0.11435084670161899 1117 48-49 0.16816283078766503 1117 50-51 0.09523828012513036 1117 52-53 0.7926982209545841 1117 54-55 0.36042453653490014 1117 56-57 0.18380492747871813 1117 58-59 0.12815954751629732 1117 60-61 0.2203184408947365 1117 62-63 0.036640193845940416 1117 64-65 0.71576711269595 1117 66-67 0.43024796287628675 1117 68-69 0.25936142058589695 1117 70-71 0.15474477297946976 1117 72-73 0.26794858334844207 1117 74-75 0.4016987714444724 1117 76 0.6834615356704674 2117 1 0.11426554172712855 2117 2 0.0414223366356552 2117 3 0.043094936329907796 2117 4 0.06283818589705703 2117 5 0.14094115523167972 2117 6 -0.09653992925929344 2117 7 0.1516627554438088 2117 8 0.1531210537100165 2117 9 0.08255467645896175 2117 10-11 0.07516813663026056 2117 12-13 0.06910090676048952 2117 14-15 -0.057336695515424196 2117 16-17 -0.026231621121404203 2117 18-19 0.43851237654299524 2117 20-21 0.23687421925150431 2117 22-23 0.1552980227336178 2117 24-25 0.08241266065009256 2117 26-27 0.1383519109649498 2117 28-29 0.08988404391794802 2117 30-31 0.47243050462029146 2117 32-33 0.38781980626674084 2117 34-35 0.36338593868635627 2117 36-37 0.22017327294062028 2117 38-39 0.2333093981619001 2117 40-41 0.6942258469348914 2117 42-43 0.21551316744274374 2117 44-45 0.20316118803485494 2117 46-47 0.19795010615739983 2117 48-49 0.26537640117226147 2117 50-51 0.15923843437023777 2117 52-53 0.8554271523502948 2117 54-55 0.4090959118690094 2117 56-57 0.19415492138017498 2117 58-59 0.2429083144984503 2117 60-61 0.14028775533807192 2117 62-63 0.14009170073269672 2117 64-65 0.7596075142697529 2117 66-67 0.4376025417976095 2117 68-69 0.24444763647959888 2117 70-71 0.29621707194003477 2117 72-73 0.26513025684925395 2117 74-75 0.37256465657461746 2117 76 0.7753201599633215 1116 1 0.08180750650688395 1116 2 0.0014696892136001338 1116 3 0.07018966144526928 1116 4 0.013450329337523215 1116 5 0.018091054017055797 1116 6 -0.14506114239905799 1116 7 0.1306235901051167 1116 8 0.10697329796759902 1116 9 0.07084257852272202 1116 10-11 0.0333344981183501 1116 12-13 0.06841825943740076 1116 14-15 0.1366460534667695 1116 16-17 -0.007977598156614363 1116 18-19 0.5937177804261609 1116 20-21 0.20841593965359095 1116 22-23 0.15342780807878142 1116 24-25 0.1273957018120484 1116 26-27 0.0790299214857697 1116 28-29 0.06298478320655221 1116 30-31 0.41479651579457766 1116 32-33 0.33097086186257485 1116 34-35 0.2472735204258214 1116 36-37 0.16922304296227253 1116 38-39 0.17490416042871004 1116 40-41 0.8183420115370907 1116 42-43 0.0961116302618592 1116 44-45 0.12810684041842535 1116 46-47 0.16994540125812563 1116 48-49 0.22190579282951006 1116 50-51 0.23334942460637365 1116 52-53 0.721844099246205 1116 54-55 0.36440987261058666 1116 56-57 -0.10448105869592439 1116 58-59 0.05997812047063178 1116 60-61 0.002942761985913478 1116 62-63 0.1854896975401914 1116 64-65 0.5953781669091072 1116 66-67 0.29743083083685207 1116 68-69 0.15121054970975223 1116 70-71 0.22361717271018478 1116 72-73 0.3088427689025153 1116 74-75 0.4453119246015831 1116 76 0.5074986620069879 2118 1 -0.15082738526637485 2118 2 -0.016661518767925543 2118 3 0.058570030702270515 2118 4 -0.10393670790649168 2118 5 0.07744623263818795 2118 6 -0.21152038982317123 2118 7 0.1350350037879906 2118 8 0.012200507738768351 2118 9 0.005957256539289801 2118 10-11 -0.04334595753881132 2118 12-13 0.058085469734926676 2118 14-15 0.0671705875646822 2118 16-17 0.05010248265010375 2118 18-19 0.6051200365580911 2118 20-21 0.256130228961041 2118 22-23 0.04992828088937529 2118 24-25 0.0477139357441132 2118 26-27 0.09939440374025565 2118 28-29 0.0010396610511804738 2118 30-31 0.3473650583402801 2118 32-33 0.35065523642584395 2118 34-35 0.3059616046825582 2118 36-37 0.17321090929471694 2118 38-39 0.05141241541695507 2118 40-41 0.7342445842364143 2118 42-43 0.09323847509352845 2118 44-45 0.03441217828936516 2118 46-47 0.24475218771014084 2118 48-49 0.1913123776311778 2118 50-51 0.20572092414639087 2118 52-53 0.7310547445722904 2118 54-55 0.26253898929844155 2118 56-57 -0.04200093264862659 2118 58-59 0.017491877951520962 2118 60-61 0.14248980826472035 2118 62-63 0.21941323348053743 2118 64-65 0.7033368927174379 2118 66-67 0.27110437098311735 2118 68-69 0.1355160611472357 2118 70-71 0.1936599322117445 2118 72-73 0.1328287371631518 2118 74-75 0.397142085570394 2118 76 0.4312821262453639 1119 1 0.022034915509976827 1119 2 -0.012834193889261769 1119 3 -0.059775173820092675 1119 4 0.05012354457559809 1119 5 -0.02579476019952409 1119 6 -0.22384884239869507 1119 7 0.13719738019661065 1119 8 0.07992679758625343 1119 9 0.05994446079229476 1119 10-11 0.0800226108868003 1119 12-13 0.022470921353786366 1119 14-15 -0.057158900753442765 1119 16-17 -0.03392381258625221 1119 18-19 0.4509175187042871 1119 20-21 0.15803493311815942 1119 22-23 0.11881184914285825 1119 24-25 0.10989077215312193 1119 26-27 -0.0197731964385639 1119 28-29 0.09857830452929761 1119 30-31 0.2580566884262723 1119 32-33 0.3418445757511037 1119 34-35 0.1453504396781966 1119 36-37 0.03446179623006884 1119 38-39 0.08108025490579962 1119 40-41 0.6472301028931398 1119 42-43 0.04617494766627317 1119 44-45 0.025071748273830963 1119 46-47 0.20835874715163527 1119 48-49 -0.017963132436896956 1119 50-51 0.1292636414145818 1119 52-53 0.45735857532347524 1119 54-55 0.15820043312167087 1119 56-57 -0.002550064308550759 1119 58-59 0.15103494521011385 1119 60-61 0.1563070283973076 1119 62-63 0.2920107272712755 1119 64-65 0.7013155346353059 1119 66-67 0.29095130006680137 1119 68-69 0.16709666356739206 1119 70-71 0.19066307811840488 1119 72-73 0.07518791529738422 1119 74-75 0.43213675026119347 1119 76 0.6771767102830069 2119 1 0.06322490904261713 2119 2 0.0765943434475318 2119 3 0.019999984767217427 2119 4 0.0492362602998071 2119 5 0.1493967357584438 2119 6 -0.029617946346625956 2119 7 0.03323602924029245 2119 8 0.09783475509492234 2119 9 0.039431958710657966 2119 10-11 0.059018446162525606 2119 12-13 0.07221716299896741 2119 14-15 0.19802098673418556 2119 16-17 0.14035709860607426 2119 18-19 0.728394170153372 2119 20-21 0.28802840654753226 2119 22-23 0.22439833322020775 2119 24-25 0.08689278309287829 2119 26-27 0.1903791737586431 2119 28-29 0.1142217545527302 2119 30-31 0.48370822406442215 2119 32-33 0.32069480831392383 2119 34-35 0.33009266244515345 2119 36-37 0.18031548115894225 2119 38-39 0.12405449003934166 2119 40-41 0.7376693826277574 2119 42-43 0.19787439900766657 2119 44-45 0.19099859227945615 2119 46-47 0.27714674124673166 2119 48-49 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0.033574089268320506 2111 5 0.0030444392222506167 2111 6 0.08336404300388267 2111 7 -0.0313131226903991 2111 8 0.0780087110750074 2111 9 -0.012774491801479826 2111 10-11 -0.11112411644191411 2111 12-13 -0.025363221713874395 2111 14-15 -0.0033432717572665638 2111 16-17 -0.019388283053132227 2111 18-19 0.559610224628571 2111 20-21 0.06625043403948894 2111 22-23 -0.07056828908176982 2111 24-25 -0.045500989810619785 2111 26-27 -0.04970118982346605 2111 28-29 -0.0060056552785425765 2111 30-31 -12.204935574638213 2111 32-33 -10.480565506673422 2111 34-35 -5.176532615522145 2111 36-37 -1.2066607987683042 2111 38-39 -0.2308636121897223 2111 40-41 -6.88037546915152 2111 42-43 -0.6925605970875779 2111 44-45 0.008078703668843445 2111 46-47 -0.1367636633053948 2111 48-49 -0.1090200733012523 2111 50-51 0.023181820985143986 2111 52-53 0.31282303001759004 2111 54-55 -0.006403510779335875 2111 56-57 0.013390877727715633 2111 58-59 0.04851739505173214 2111 60-61 0.026943778860733403 2111 62-63 -0.05845746753972492 2111 64-65 0.40629302492816066 2111 66-67 -0.043683542680703624 2111 68-69 0.03210996986620529 2111 70-71 -0.09406815593877127 2111 72-73 -0.02856748145271837 2111 74-75 0.06952250122422754 2111 76 -0.0177231267448974 2110 1 0.10312662042243659 2110 2 -0.08772078845341014 2110 3 0.0015959251724524393 2110 4 0.02066048886993599 2110 5 -0.103172246810324 2110 6 0.09426347401515756 2110 7 -0.11778131188606267 2110 8 -0.25645533750574856 2110 9 -0.18627052179096637 2110 10-11 -0.0654370648548337 2110 12-13 0.03397442975517606 2110 14-15 0.030154325948331007 2110 16-17 -0.12818657101491482 2110 18-19 0.6157285825842678 2110 20-21 0.06218169199334511 2110 22-23 -0.08836705427477654 2110 24-25 -0.13408813177192513 2110 26-27 -0.07219843479295207 2110 28-29 -0.06381904864618093 2110 30-31 -0.012167875584367494 2110 32-33 -0.34500832192268405 2110 34-35 -0.1765311169699757 2110 36-37 -0.0798536024902532 2110 38-39 -0.1492995502372736 2110 40-41 0.31832666939769894 2110 42-43 -0.15318723396762834 2110 44-45 -0.30374055019050417 2110 46-47 -0.3612068935383306 2110 48-49 -0.2709410557981613 2110 50-51 -0.32888329917568626 2110 52-53 0.34144964153752966 2110 54-55 -0.047921993691232956 2110 56-57 -0.10972815111826151 2110 58-59 -0.20116762080986916 2110 60-61 -0.3494253214546461 2110 62-63 -0.17303607467006543 2110 64-65 0.4323196484365255 2110 66-67 -0.08343834496403701 2110 68-69 -0.4253575461337178 2110 70-71 -0.24128937912621495 2110 72-73 -0.26437169676469097 2110 74-75 -0.08064447206927383 2110 76 -0.6437766089190475 2109 1 -0.15931336097364834 2109 2 -0.06714390665737113 2109 3 -0.012357089451612069 2109 4 -0.12200598410873909 2109 5 -0.1745220608945246 2109 6 0.049754328117515456 2109 7 -0.09341556295505171 2109 8 -0.1510411875492963 2109 9 -0.05096457803697518 2109 10-11 -0.029151177060633415 2109 12-13 -0.023830525511534972 2109 14-15 -0.032164245769273236 2109 16-17 -0.07017520541587885 2109 18-19 0.46033028543482146 2109 20-21 0.14507633590352498 2109 22-23 -0.10400203938424823 2109 24-25 -0.07553043804075799 2109 26-27 -0.16820025046365572 2109 28-29 -0.09206792568761557 2109 30-31 0.19545844260428424 2109 32-33 0.1397079066636877 2109 34-35 0.11607399577617628 2109 36-37 -0.002399031268588203 2109 38-39 -0.26749406202016246 2109 40-41 0.14204029429931353 2109 42-43 0.06227527476066541 2109 44-45 -0.05941775959066575 2109 46-47 -0.21554504193047563 2109 48-49 -0.09306986263078443 2109 50-51 -0.09327797176895558 2109 52-53 0.17483563764897525 2109 54-55 -0.21836522923063484 2109 56-57 0.015480996444850348 2109 58-59 -0.04918072281559205 2109 60-61 -0.18296367722864915 2109 62-63 -0.004715195351252532 2109 64-65 0.6124101574781733 2109 66-67 -0.14181302975841703 2109 68-69 -0.1584412285989103 2109 70-71 -0.24381858036680626 2109 72-73 -0.2746779489390505 2109 74-75 -0.020468072581010688 2109 76 -0.6031315076214625 2108 1 -0.05258560632894671 2108 2 -0.01671431987460892 2108 3 0.015201831768742124 2108 4 -0.027829511538058682 2108 5 0.0883304335946562 2108 6 0.026638304480776753 2108 7 0.09764592247504567 2108 8 0.08595863268340054 2108 9 -0.008558504909856879 2108 10-11 0.01348006791546652 2108 12-13 0.0059655770315600876 2108 14-15 0.02807451621010415 2108 16-17 -0.05222317297724999 2108 18-19 0.538840839688028 2108 20-21 0.08721418865829378 2108 22-23 -0.030332708804209574 2108 24-25 -0.06580676992096102 2108 26-27 -0.05432115434692264 2108 28-29 -0.004697507951135549 2108 30-31 0.35118028698441606 2108 32-33 0.14158331689521475 2108 34-35 0.1904100887548168 2108 36-37 0.05053044696742148 2108 38-39 0.006618586603089227 2108 40-41 0.4796045923434704 2108 42-43 0.05786195279279838 2108 44-45 -0.12050991497284969 2108 46-47 0.01624378231193191 2108 48-49 0.0399732877684329 2108 50-51 0.018175557535151654 2108 52-53 0.4228512179445332 2108 54-55 -0.0976483625770257 2108 56-57 -0.11341040459332419 2108 58-59 -0.04278014697410981 2108 60-61 -0.16609765214590766 2108 62-63 -0.07099361040364727 2108 64-65 0.3936308059931548 2108 66-67 0.019807067716946847 2108 68-69 0.01912369257686919 2108 70-71 -0.17037540491892855 2108 72-73 -0.19080100139246525 2108 74-75 0.1421630906476281 2108 76 -0.9490883589960077 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 2.0 11 1.0 12 40.0 13 287.0 14 1060.0 15 1865.0 16 2502.0 17 3135.0 18 4381.0 19 6069.0 20 6676.0 21 6536.0 22 6358.0 23 6185.0 24 6303.0 25 6729.0 26 7310.0 27 8148.0 28 9442.0 29 11420.0 30 14552.0 31 19988.0 32 30517.0 33 44092.0 34 69655.0 35 118289.0 36 294267.0 37 1213757.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.139820358966205 2.2567786536503602 45.39484282199197 5.208558165391463 2 3.2267370546745946 2.2864170026205985 4.770984530150571 89.71586141255423 3 3.330181736249228 42.21595880322136 4.890169649277783 49.56368981125162 4 3.447419042033812 2.1887631174699904 89.39778875806368 4.966029082432514 5 3.278906866094676 2.440452187499671 44.739587884811584 49.54105306159407 6 43.719407485709894 2.3334277408629127 5.800851352361539 48.146313421065656 7 3.4233609150721795 2.8321732437830534 46.25214391076698 47.49232193037778 8 4.436013278822636 3.3533975655491837 89.56872253978014 2.641866615848041 9 5.528104840789949 2.9348282712998652 88.4657337518149 3.071333136095298 10-11 66.86487860911387 25.523119491504904 3.3559244585342123 4.256077440847014 12-13 25.194728690658813 23.790328948823046 25.9255271993708 25.08941516114734 14-15 3.6419634800791334 45.45762031958879 24.467983739443643 26.432432460888432 16-17 23.71494330810301 3.5828447129502212 46.28767834336896 26.414533635577808 18-19 26.065138036793666 45.00514854445699 4.539773822020398 24.389939596728937 20-21 24.358406078019925 2.352379438250632 48.81628224552345 24.472932238205992 22-23 25.270588123813543 2.796480880369516 44.0984677552662 27.83446324055074 24-25 4.358074423315641 23.747503377087188 25.437520991637037 46.45690120796013 26-27 25.252215506068225 24.388255001405586 17.60433699065997 32.75519250186621 28-29 18.22482082749428 9.562500065804505 25.70908302212189 46.50359608457932 30-31 46.93314157023236 11.515762021430158 23.439459329130973 18.111637079206513 32-33 51.4780481436286 17.72425912024115 4.647298382893776 26.15039435323648 34-35 3.7845223593178647 44.64867238095439 24.9898134626541 26.576991797073646 36-37 17.673931834955987 9.804239494705632 39.85865718801031 32.66317148232807 38-39 46.73917621182944 3.6490966884014555 46.587957459756595 3.023769640012508 40-41 25.817107697231894 38.09786024807772 26.49542053290067 9.589611521789713 42-43 31.491403825926557 3.051723393659394 55.25849062364772 10.198382156766336 44-45 3.599795953391459 4.891775279195353 81.46931983410948 10.039108933303712 46-47 10.588892410161058 73.50918578243662 11.212297967009306 4.689623840393016 48-49 10.484000029480418 26.344649251460595 37.709771600460314 25.46157911859867 50-51 37.854067718626254 18.653839877108773 32.22794048745871 11.26415191680626 52-53 44.91415407519402 27.063313409484063 23.72813053086863 4.294401984453291 54-55 18.416390901921808 53.15553658046102 4.051241178248084 24.376831339369097 56-57 24.235009470584334 24.009089444641564 12.050515749386966 39.705385335387135 58-59 2.6319169747194886 24.456270537585954 48.38510480815091 24.526707679543645 60-61 9.905367857710656 39.31700714794853 25.514196400651517 25.263428593689298 62-63 3.5213306618459166 18.542603942163634 53.285987430813144 24.65007796517731 64-65 32.78393590957092 37.63312251324776 25.669258135805755 3.913683441375556 66-67 25.30646473984057 26.32969846796584 23.706441366080462 24.657395426113123 68-69 4.305141279639665 24.45619157218017 45.41126762639466 25.827399521785505 70-71 47.813605844703474 5.375675285828447 23.356703583871262 23.454015285596817 72-73 39.528186964811965 52.55221455848336 3.7590165332502266 4.160581943454452 74-75 16.38326859924846 31.664390708193345 24.88623717206983 27.066103520488365 76 17.112593087052517 72.82905674243484 3.408410131577423 6.64994003893521 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 1.0 4 2.0 5 1.0 6 0.0 7 0.0 8 0.0 9 1.5 10 1.5 11 0.0 12 0.0 13 0.5 14 1.5 15 2.0 16 4.0 17 6.0 18 5.5 19 12.0 20 17.0 21 15.0 22 23.0 23 35.0 24 59.5 25 80.0 26 96.5 27 197.0 28 505.5 29 730.0 30 1671.5 31 3413.5 32 17328.5 33 30443.0 34 118762.5 35 132057.5 36 141713.5 37 226394.0 38 135185.0 39 31256.5 40 23948.5 41 119886.5 42 210413.0 43 121963.0 44 26207.5 45 17261.0 46 15620.0 47 27624.5 48 135487.5 49 156825.5 50 82305.0 51 158059.5 52 149861.5 53 144630.5 54 223352.0 55 131318.5 56 24705.5 57 7897.5 58 5669.0 59 5083.0 60 4463.0 61 4550.0 62 4671.0 63 4918.0 64 5339.5 65 5615.5 66 5848.5 67 5980.0 68 5883.5 69 5629.0 70 5263.0 71 5055.0 72 4593.0 73 3846.5 74 3157.0 75 2752.0 76 2378.0 77 1713.0 78 1153.5 79 885.0 80 733.0 81 475.5 82 283.5 83 197.0 84 166.0 85 105.5 86 66.0 87 56.0 88 49.5 89 32.5 90 19.5 91 14.0 92 11.0 93 16.5 94 15.5 95 13.5 96 18.0 97 17.0 98 15.0 99 43.5 100 73.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 1899566.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 9.223346974647297 #Duplication Level Percentage of deduplicated Percentage of total 1 91.3307712441788 8.423753926472008 2 4.049739450655427 0.7470430422062507 3 1.515278914713606 0.4192782958131173 4 0.7586025129474695 0.27987416771015516 5 0.37107682946890314 0.17112851762218592 6 0.2677709981709314 0.14818468955268882 7 0.18996632535070893 0.12264877325458275 8 0.12796147584491524 0.09441864728844816 9 0.11345598681838498 0.0941799539499277 >10 0.8153270212229724 1.5599642078076694 >50 0.17449334202506575 1.1371006242764898 >100 0.1900615838279651 3.8585017205760677 >500 0.052141067668458135 3.188899783101759 >1k 0.0380805548909305 8.42417459223018 >5k 0.0017575640718890998 0.8809393036422385 >10k+ 0.0035151281437781996 70.44990975449625 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAA 231449 12.184309468583876 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAA 227892 11.99705616967244 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCAC 221117 11.640395753556339 No Hit TCATTGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTG 210318 11.07189747552862 No Hit TCATTGTTTGGCATAGTCACGTTCAGCACTGGATATGTGCTTCCTGATTT 209226 11.014410660119205 No Hit TCATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTG 203771 10.727239801091407 No Hit ATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGG 5506 0.28985568282439256 No Hit TCATTGTTTGGCATAGTCACGTTCAGTGCTAGATATTTGTATTTTAATTG 5462 0.2875393642547824 No Hit TCATTGTTTGGCATAGTCACGTTCAATGCTAGGTATGTGTAGTTTAAGTG 5335 0.28085362656522594 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCATCCAGCACACTGGAGTTTAACAA 4525 0.23821230744285798 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCATCCAGCACCCTGGAGTTTAACAA 4499 0.23684357374263387 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGTAGTTTATCAC 4419 0.23263208543425185 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGTAGTTTAACAA 4287 0.2256831297254215 No Hit GCCTCCCTTAGGTCACTAATTGCCTCGTCAGGCACTCTGGAGTTTATCAC 4239 0.22315623674039226 No Hit CTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCACTG 4220 0.22215600826715154 No Hit GCCTCCCTTAGGTCACTAATTGCCTCATCCGGCACACTGGAGTTTAACAA 4175 0.21978704609368666 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGTAGTTTAACAA 4154 0.21868153041273639 No Hit TGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTCAACC 4137 0.21778658914720522 No Hit CTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAATG 4016 0.2114167130807774 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCGTCAAGCACTCTGGAGTTTATCAC 3968 0.2088898200957482 No Hit TCATTGTTTGGCATAGTCACGTTCAGCACTAGATATGTGCTTCCTGATTT 3967 0.2088371764918934 No Hit GCCTCCCTTAGGTCACTAATTGCCTCATCCGGCACCCTGGAGTTTAACAA 3963 0.2086266020764743 No Hit TGGCATAGTCACGTTCAGCACTGGATATGTGCTTCCTGATTTGGTCAACC 3619 0.19051720235043162 No Hit CTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAATG 3534 0.18604249602277573 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTAACAA 3308 0.17414504155159652 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTAACAC 3293 0.17335538749377488 No Hit TCATTGTTTGGCATAGTCGCGTTCAGTGCTGGATATTTGTATTTTAATTG 3159 0.166301144577235 No Hit CATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGG 3093 0.16282666672281984 No Hit TCATTGTTTGGCATAGTCACTCAAAACGGAACAAGTTCTGCTTGCATAAG 2984 0.15708851390264933 No Hit TCATTGTTTGGCATAGTCGCGTTCAGCACTGGATATGTGCTTCCTGATTT 2873 0.1512450738747693 No Hit TCATTGTTTGGCATAGTCACGTTCAGCACTGGATATGTGTTTCCTGATTT 2832 0.1490866861167235 No Hit TCATTGTTTGGACTGGAGTCACTCAAAACGGAACAAGTTCTGCTTGCATA 2823 0.14861289368203054 No Hit GCCTCCCTTAGGTCACTAGTTGAACGAAGCAAAGCCTACAGCAACTGTTA 2730 0.14371703852353643 No Hit CCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCACT 2689 0.14155865076549065 No Hit CATTGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTGG 2396 0.1261340748360415 No Hit TTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGT 2341 0.12323867662402885 No Hit CCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAAT 2273 0.11965891156190413 No Hit TGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTC 2249 0.11839546506938953 No Hit CCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAAT 2227 0.11723730578458448 No Hit TTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTCAAC 2175 0.11449983838413616 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACCCTGGAGTTTATCAC 2088 0.10991984484877072 No Hit CATTGTTTGGCATAGTCACGTTCAGCACTGGATATGTGCTTCCTGATTTG 2028 0.1067612286174842 No Hit TCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAATGA 2007 0.10565571293653392 No Hit ATTGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTGGT 1969 0.10365525599005246 No Hit TTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTGGTGCAAC 1917 0.10091778858960414 No Hit TTGGCATAGTCACGTTCAGCACTGGATATGTGCTTCCTGATTTGGTCAAC 1909 0.10049663975876595 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.00210574415419101 0.0 0.0 2 0.00210574415419101 0.0 0.0 3 0.0021583877580457853 0.0 0.0 4 0.0021583877580457853 0.0 0.0 5 0.0021583877580457853 0.0 0.0 6 0.0021583877580457853 0.0 0.0 7 0.0021583877580457853 0.0 0.0 8 0.0021583877580457853 0.0 0.0 9 0.0021583877580457853 0.0 0.0 10 0.0021583877580457853 0.0 0.0 11 0.0021583877580457853 0.0 0.0 12 0.0021583877580457853 0.0 0.0 13 0.0022636749657553358 0.0 0.0 14 0.0022636749657553358 0.0 0.0 15 0.002368962173464886 0.0 0.0 16 0.002368962173464886 0.0 0.0 17 0.002368962173464886 0.0 0.0 18 0.002368962173464886 0.0 0.0 19 0.002368962173464886 0.0 0.0 20 0.002368962173464886 0.0 0.0 21 0.002368962173464886 0.0 0.0 22 0.002368962173464886 0.0 0.0 23 0.002368962173464886 0.0 0.0 24 0.0024216057773196616 0.0 0.0 25 0.0024216057773196616 0.0 0.0 26 0.0024742493811744366 0.0 0.0 27 0.0024742493811744366 0.0 0.0 28 0.0026321801927387625 0.0 0.0 29 0.0026321801927387625 0.0 0.0 30 0.0026848237965935375 0.0 0.0 31 0.002737467400448313 0.0 0.0 32 0.0028953982120126388 0.0 0.0 33 0.0030533290235769646 0.0 0.0 34 0.00321125983514129 0.0 0.0 35 0.003369190646705616 0.0 0.0 36 0.0035797650621247168 0.0 0.0 37 0.003790339477543818 0.0 0.0 38 0.0038956266852533685 0.0 0.0 39 0.004369419119946346 0.0 0.0 40 0.004790567950784547 0.0 0.0 41 0.005159073177767974 0.0 0.0 42 0.005632865612460952 0.0 0.0 43 0.00642251967028258 0.0 0.0 44 0.006738381293411232 0.0 0.0 45 0.00742274814352331 0.0 0.0 46 0.00831768940905449 0.0 0.0 47 0.009265274278440443 0.0 0.0 48 0.009949641128552521 0.0 0.0 49 0.010791938790228927 0.0 0.0 50 0.012002741678888757 0.0 0.0 51 0.012897682944419935 0.0 0.0 52 0.013687337002241565 0.0 0.0 53 0.015424575929449147 0.0 0.0 54 0.016688022421963752 0.0 0.0 55 0.01805675612218791 0.0 0.0 56 0.01916227180313819 0.0 0.0 57 0.020425718295652796 0.0 0.0 58 0.02205767001515083 0.0 5.264360385477525E-5 59 0.023742265338503637 0.0 5.264360385477525E-5 60 0.02569007868113032 0.0 5.264360385477525E-5 61 0.027164099589064027 0.0 5.264360385477525E-5 62 0.02848018968543341 0.0 5.264360385477525E-5 63 0.030059497801076668 0.0 5.264360385477525E-5 64 0.03158616231286515 5.264360385477525E-5 5.264360385477525E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCATTAA 35 3.7161954E-9 70.00001 53 ACAATTA 55 0.0 70.00001 47 TTAGGTT 65 0.0 70.0 8 TTCTAAG 15 0.0022251287 70.0 42 AGGTAAC 15 0.0022251287 70.0 10 ATAGGGG 15 0.0022251287 70.0 33 ATTACAC 15 0.0022251287 70.0 62 AGAGTAA 15 0.0022251287 70.0 39 CCGTAAC 15 0.0022251287 70.0 48 TCTGTAA 15 0.0022251287 70.0 28 ACGGACC 15 0.0022251287 70.0 26 AATCTAG 15 0.0022251287 70.0 59 TAGGGGG 15 0.0022251287 70.0 34 GAGCCTA 15 0.0022251287 70.0 40 GTATCAT 30 1.0246549E-7 70.0 50 TGCTATA 15 0.0022251287 70.0 27 ACATATT 20 7.929946E-5 70.0 3 ACATACT 15 0.0022251287 70.0 11 CCCGGGT 15 0.0022251287 70.0 1 ATATTGT 20 7.929946E-5 70.0 33 >>END_MODULE