FastQCFastQC Report
Tue 25 Aug 2015
000000000-AHV5L l01n02 kokuya3.34200000033cf6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AHV5L l01n02 kokuya3.34200000033cf6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1899566
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAA23144912.184309468583876No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAA22789211.99705616967244No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCAC22111711.640395753556339No Hit
TCATTGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTG21031811.07189747552862No Hit
TCATTGTTTGGCATAGTCACGTTCAGCACTGGATATGTGCTTCCTGATTT20922611.014410660119205No Hit
TCATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTG20377110.727239801091407No Hit
ATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGG55060.28985568282439256No Hit
TCATTGTTTGGCATAGTCACGTTCAGTGCTAGATATTTGTATTTTAATTG54620.2875393642547824No Hit
TCATTGTTTGGCATAGTCACGTTCAATGCTAGGTATGTGTAGTTTAAGTG53350.28085362656522594No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCATCCAGCACACTGGAGTTTAACAA45250.23821230744285798No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCATCCAGCACCCTGGAGTTTAACAA44990.23684357374263387No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGTAGTTTATCAC44190.23263208543425185No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGTAGTTTAACAA42870.2256831297254215No Hit
GCCTCCCTTAGGTCACTAATTGCCTCGTCAGGCACTCTGGAGTTTATCAC42390.22315623674039226No Hit
CTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCACTG42200.22215600826715154No Hit
GCCTCCCTTAGGTCACTAATTGCCTCATCCGGCACACTGGAGTTTAACAA41750.21978704609368666No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGTAGTTTAACAA41540.21868153041273639No Hit
TGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTCAACC41370.21778658914720522No Hit
CTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAATG40160.2114167130807774No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCGTCAAGCACTCTGGAGTTTATCAC39680.2088898200957482No Hit
TCATTGTTTGGCATAGTCACGTTCAGCACTAGATATGTGCTTCCTGATTT39670.2088371764918934No Hit
GCCTCCCTTAGGTCACTAATTGCCTCATCCGGCACCCTGGAGTTTAACAA39630.2086266020764743No Hit
TGGCATAGTCACGTTCAGCACTGGATATGTGCTTCCTGATTTGGTCAACC36190.19051720235043162No Hit
CTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAATG35340.18604249602277573No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTAACAA33080.17414504155159652No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTAACAC32930.17335538749377488No Hit
TCATTGTTTGGCATAGTCGCGTTCAGTGCTGGATATTTGTATTTTAATTG31590.166301144577235No Hit
CATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGG30930.16282666672281984No Hit
TCATTGTTTGGCATAGTCACTCAAAACGGAACAAGTTCTGCTTGCATAAG29840.15708851390264933No Hit
TCATTGTTTGGCATAGTCGCGTTCAGCACTGGATATGTGCTTCCTGATTT28730.1512450738747693No Hit
TCATTGTTTGGCATAGTCACGTTCAGCACTGGATATGTGTTTCCTGATTT28320.1490866861167235No Hit
TCATTGTTTGGACTGGAGTCACTCAAAACGGAACAAGTTCTGCTTGCATA28230.14861289368203054No Hit
GCCTCCCTTAGGTCACTAGTTGAACGAAGCAAAGCCTACAGCAACTGTTA27300.14371703852353643No Hit
CCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCACT26890.14155865076549065No Hit
CATTGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTGG23960.1261340748360415No Hit
TTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGT23410.12323867662402885No Hit
CCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAAT22730.11965891156190413No Hit
TGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTC22490.11839546506938953No Hit
CCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAAT22270.11723730578458448No Hit
TTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTCAAC21750.11449983838413616No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACCCTGGAGTTTATCAC20880.10991984484877072No Hit
CATTGTTTGGCATAGTCACGTTCAGCACTGGATATGTGCTTCCTGATTTG20280.1067612286174842No Hit
TCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAATGA20070.10565571293653392No Hit
ATTGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTGGT19690.10365525599005246No Hit
TTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTGGTGCAAC19170.10091778858960414No Hit
TTGGCATAGTCACGTTCAGCACTGGATATGTGCTTCCTGATTTGGTCAAC19090.10049663975876595No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATTAA353.7161954E-970.0000153
ACAATTA550.070.0000147
TTAGGTT650.070.08
TTCTAAG150.002225128770.042
AGGTAAC150.002225128770.010
ATAGGGG150.002225128770.033
ATTACAC150.002225128770.062
AGAGTAA150.002225128770.039
CCGTAAC150.002225128770.048
TCTGTAA150.002225128770.028
ACGGACC150.002225128770.026
AATCTAG150.002225128770.059
TAGGGGG150.002225128770.034
GAGCCTA150.002225128770.040
GTATCAT301.0246549E-770.050
TGCTATA150.002225128770.027
ACATATT207.929946E-570.03
ACATACT150.002225128770.011
CCCGGGT150.002225128770.01
ATATTGT207.929946E-570.033
Produced by FastQC (version 0.11.2)