##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AHV5L l01n02 kokuya2.34200000033ce9.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2383239 Sequences flagged as poor quality 0 Sequence length 76 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.94291718119752 34.0 34.0 34.0 32.0 34.0 2 33.36270848202803 34.0 34.0 34.0 34.0 34.0 3 33.27360159849684 34.0 34.0 34.0 34.0 34.0 4 33.3277447205253 34.0 34.0 34.0 34.0 34.0 5 33.261995125121736 34.0 34.0 34.0 34.0 34.0 6 36.852152050214016 38.0 38.0 38.0 36.0 38.0 7 36.934366632973024 38.0 38.0 38.0 36.0 38.0 8 37.06440143015451 38.0 38.0 38.0 37.0 38.0 9 37.171075582432145 38.0 38.0 38.0 37.0 38.0 10-11 36.97830410630239 38.0 38.0 38.0 36.5 38.0 12-13 36.978749088949954 38.0 38.0 38.0 37.0 38.0 14-15 36.88936338319405 38.0 38.0 38.0 37.0 38.0 16-17 37.03690502715003 38.0 38.0 38.0 37.0 38.0 18-19 36.29628333541034 38.0 38.0 38.0 34.0 38.0 20-21 36.77553992696494 38.0 38.0 38.0 35.5 38.0 22-23 36.74398329332476 38.0 37.5 38.0 36.0 38.0 24-25 36.94974129745275 38.0 38.0 38.0 36.5 38.0 26-27 36.489794561099416 38.0 38.0 38.0 34.5 38.0 28-29 36.862753378909964 38.0 38.0 38.0 36.5 38.0 30-31 36.437698862766176 38.0 38.0 38.0 35.0 38.0 32-33 36.12970121754469 38.0 38.0 38.0 32.5 38.0 34-35 36.248834883954146 38.0 37.0 38.0 34.0 38.0 36-37 36.41028721836123 38.0 37.5 38.0 34.5 38.0 38-39 36.83396608565066 38.0 38.0 38.0 36.0 38.0 40-41 35.70429591828599 38.0 37.0 38.0 31.5 38.0 42-43 36.16155219849961 38.0 37.0 38.0 34.0 38.0 44-45 36.37233739461296 38.0 37.0 38.0 34.0 38.0 46-47 36.113327282744194 38.0 37.0 38.0 34.0 38.0 48-49 36.357073084151445 38.0 37.5 38.0 34.0 38.0 50-51 36.3310928950055 38.0 37.5 38.0 34.5 38.0 52-53 35.67999663483184 38.0 37.0 38.0 30.5 38.0 54-55 36.2404060188676 38.0 37.0 38.0 34.0 38.0 56-57 36.56759288514496 38.0 37.5 38.0 34.5 38.0 58-59 36.49187324477319 38.0 38.0 38.0 35.0 38.0 60-61 36.46271334935355 38.0 38.0 38.0 34.0 38.0 62-63 36.25757404104246 38.0 37.5 38.0 34.0 38.0 64-65 35.775754341045946 38.0 37.0 38.0 30.5 38.0 66-67 36.34672561165708 38.0 37.5 38.0 34.5 38.0 68-69 36.542476646278445 38.0 38.0 38.0 35.0 38.0 70-71 36.55554982106285 38.0 38.0 38.0 35.5 38.0 72-73 36.1389822841939 38.0 37.0 38.0 34.0 38.0 74-75 36.105507043145906 38.0 37.0 38.0 33.0 38.0 76 34.23048338836348 38.0 34.0 38.0 24.0 38.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.1922779214446919 1101 2 0.10948955815048578 1101 3 0.10132192151785802 1101 4 0.19041334624857598 1101 5 0.109156143192493 1101 6 0.2123343837240057 1101 7 0.1956931522345755 1101 8 0.23408017796024438 1101 9 0.22852533179346324 1101 10-11 0.19266721279127097 1101 12-13 0.15140794809259717 1101 14-15 0.20258360007512977 1101 16-17 0.17866911000074737 1101 18-19 0.6808782443162045 1101 20-21 0.26863590215147326 1101 22-23 0.286497599816137 1101 24-25 0.2080912820066203 1101 26-27 0.28625109897864576 1101 28-29 0.18637513023988816 1101 30-31 0.5360816954839294 1101 32-33 0.566304577746223 1101 34-35 0.4158365723104538 1101 36-37 0.2614557209212336 1101 38-39 0.19567440358208188 1101 40-41 0.8160503263816778 1101 42-43 0.4902223373283192 1101 44-45 0.33402562592548435 1101 46-47 0.3161736842591978 1101 48-49 0.3006219356337283 1101 50-51 0.2728568536427076 1101 52-53 0.7686258559647499 1101 54-55 0.4335598638185516 1101 56-57 0.18626579413241018 1101 58-59 0.2199260040255453 1101 60-61 0.2847461917397851 1101 62-63 0.3565823046759675 1101 64-65 0.8019532797859483 1101 66-67 0.3809344314133014 1101 68-69 0.27426316204970647 1101 70-71 0.17079810901973502 1101 72-73 0.19754792417848677 1101 74-75 0.5517317183599744 1101 76 0.5835026491854478 1102 1 0.15280846721809382 1102 2 0.09862686423068112 1102 3 0.15810918812988461 1102 4 0.17543967050955445 1102 5 0.1548459240097415 1102 6 0.24501822395327366 1102 7 0.21899625267677436 1102 8 0.23317737894620194 1102 9 0.17636763365386798 1102 10-11 0.24647501792328086 1102 12-13 0.2707640702242742 1102 14-15 0.22022054797918145 1102 16-17 0.2638126547973272 1102 18-19 0.7929881032344355 1102 20-21 0.3675517019536798 1102 22-23 0.2983753773667459 1102 24-25 0.22296918557252354 1102 26-27 0.2624796206745117 1102 28-29 0.23229434495246437 1102 30-31 0.6179772083582407 1102 32-33 0.6491272814600393 1102 34-35 0.5685985875181316 1102 36-37 0.36644359160641216 1102 38-39 0.26046122971611396 1102 40-41 0.8798476254885443 1102 42-43 0.3974467821909258 1102 44-45 0.35432468121637584 1102 46-47 0.4085361816466673 1102 48-49 0.3790221273260741 1102 50-51 0.3617176578263894 1102 52-53 0.744988121602475 1102 54-55 0.4711476805566761 1102 56-57 0.28999648546744794 1102 58-59 0.40021831895065674 1102 60-61 0.3611387854607173 1102 62-63 0.4505982937642159 1102 64-65 0.8658792937335704 1102 66-67 0.5686913986093671 1102 68-69 0.37307506363805487 1102 70-71 0.404842429350289 1102 72-73 0.35508536549354375 1102 74-75 0.6300434143170293 1102 76 0.8253178077128496 1103 1 0.21350296970443594 1103 2 0.06252815758114139 1103 3 0.0917184635119952 1103 4 0.07797600218627565 1103 5 0.07857722471479178 1103 6 0.09096684819881773 1103 7 0.12870453891546418 1103 8 0.1116144397678056 1103 9 0.09215962300705627 1103 10-11 0.15426923455348884 1103 12-13 0.049164970895972715 1103 14-15 0.10420531114579035 1103 16-17 0.1357367030533183 1103 18-19 0.6727311478632458 1103 20-21 0.25252128070243884 1103 22-23 0.19705596686747384 1103 24-25 0.09232780004295194 1103 26-27 0.17488189005904786 1103 28-29 0.1440770660581805 1103 30-31 0.48075179214445996 1103 32-33 0.4351708369050016 1103 34-35 0.42311613867770603 1103 36-37 0.2012448059834071 1103 38-39 0.10987951034886123 1103 40-41 0.7709631439541837 1103 42-43 0.40147000238002306 1103 44-45 0.3160466812835736 1103 46-47 0.2958885058158174 1103 48-49 0.25908959563300726 1103 50-51 0.3180845744133691 1103 52-53 0.7532631549695026 1103 54-55 0.33848197772297084 1103 56-57 0.11360028184000726 1103 58-59 0.24157598840956496 1103 60-61 0.16858024229291857 1103 62-63 0.17018144695336446 1103 64-65 0.7038251663279453 1103 66-67 0.3746429795722648 1103 68-69 0.24253574613640438 1103 70-71 0.2519756555108259 1103 72-73 0.2703486583290342 1103 74-75 0.5173145983707101 1103 76 0.6887263294274888 2116 1 0.15162008622670697 2116 2 0.12403899489056869 2116 3 0.1455205053119073 2116 4 0.10359276812288698 2116 5 0.25619045180818034 2116 6 0.20197653874323862 2116 7 0.28630285123039556 2116 8 0.16487179082872672 2116 9 0.15391903995744372 2116 10-11 0.13572741074074912 2116 12-13 0.12270332507701198 2116 14-15 0.21369193683112542 2116 16-17 0.1344365585324354 2116 18-19 0.7496791705683563 2116 20-21 0.3336329575074046 2116 22-23 0.21323734065062183 2116 24-25 0.17851782334379607 2116 26-27 0.21888866455405775 2116 28-29 0.1968002919753573 2116 30-31 0.596212699359711 2116 32-33 0.5867732150805338 2116 34-35 0.435808300098266 2116 36-37 0.16183792117809048 2116 38-39 0.17422195462823709 2116 40-41 0.7304046778744393 2116 42-43 0.36029074583239407 2116 44-45 0.27340348604558784 2116 46-47 0.23227051833174528 2116 48-49 0.21933363453788246 2116 50-51 0.15258760147013106 2116 52-53 0.6357004078203303 2116 54-55 0.35914304039791745 2116 56-57 0.16464499143241085 2116 58-59 0.19281252804704252 2116 60-61 0.18382256792477847 2116 62-63 0.1829170782515206 2116 64-65 0.7823203352163759 2116 66-67 0.38885061783350494 2116 68-69 0.3687649159281534 2116 70-71 0.2428310230500088 2116 72-73 0.21070843907619263 2116 74-75 0.4856178949516874 2116 76 0.7743289742402268 1117 1 0.26686314830711666 1117 2 0.04732209072214033 1117 3 0.03600758858328845 1117 4 0.014295004105470355 1117 5 0.08485216595151002 1117 6 0.06545665339213258 1117 7 -0.8555861693722804 1117 8 -0.05235472684390885 1117 9 0.030769909319687372 1117 10-11 0.16210227231452023 1117 12-13 0.0032813584587643163 1117 14-15 -0.5724663580221261 1117 16-17 -0.22526788249019347 1117 18-19 -0.06586882605830624 1117 20-21 0.13929341635731163 1117 22-23 0.1973706683212626 1117 24-25 0.2164557295511429 1117 26-27 0.19779085032048016 1117 28-29 0.19536195858703564 1117 30-31 0.5196339686142579 1117 32-33 0.5261958670943585 1117 34-35 0.3694081647700429 1117 36-37 0.18082110718323463 1117 38-39 0.10891140158354062 1117 40-41 0.8128678095344171 1117 42-43 0.36266773041484157 1117 44-45 0.22927398373558816 1117 46-47 0.2471558061326462 1117 48-49 0.2303533494127734 1117 50-51 0.1471243420672721 1117 52-53 0.7930436167233381 1117 54-55 0.3744950288471429 1117 56-57 0.18479812886395308 1117 58-59 0.18285179644903593 1117 60-61 0.3002669561486897 1117 62-63 0.011268078805791504 1117 64-65 0.759759600343763 1117 66-67 0.44410342006671755 1117 68-69 0.2572482560087792 1117 70-71 0.22318569892036777 1117 72-73 0.3048085294935561 1117 74-75 0.45164557290984675 1117 76 0.613273422927989 2117 1 0.11461748146405171 2117 2 0.10590418786519962 2117 3 0.10005986573577985 2117 4 0.11491288950328027 2117 5 0.21724160960461347 2117 6 -0.039012412226945514 2117 7 0.23967668731091152 2117 8 0.18654952743164444 2117 9 0.17555588336557548 2117 10-11 0.1667787034819881 2117 12-13 0.135254763225376 2117 14-15 0.03851968141872675 2117 16-17 0.12834776124091007 2117 18-19 0.5348126804298943 2117 20-21 0.32034947107544554 2117 22-23 0.2479082274658495 2117 24-25 0.18449777699161274 2117 26-27 0.25255347497380143 2117 28-29 0.19245377151092669 2117 30-31 0.5470346990134374 2117 32-33 0.4959410158936848 2117 34-35 0.42778314037578724 2117 36-37 0.271460529711554 2117 38-39 0.23062568961651664 2117 40-41 0.7274901294634262 2117 42-43 0.3159531257158079 2117 44-45 0.28366185946672573 2117 46-47 0.28122775825471535 2117 48-49 0.2583057915990068 2117 50-51 0.25695353595830284 2117 52-53 0.9326758711932186 2117 54-55 0.45823088449830607 2117 56-57 0.2417833654261159 2117 58-59 0.26355984476234795 2117 60-61 0.27589776179594594 2117 62-63 0.2390692121906426 2117 64-65 0.8854315561287365 2117 66-67 0.43302630417674237 2117 68-69 0.29108577928647605 2117 70-71 0.3473773711019632 2117 72-73 0.3640480308621292 2117 74-75 0.5337115017418483 2117 76 0.6747845577566096 1116 1 0.1439913742398673 1116 2 -0.01234452932504837 1116 3 -0.01804138457039528 1116 4 0.014659859368528316 1116 5 7.002916005873772E-4 1116 6 -0.2621568669613481 1116 7 0.12112814614635425 1116 8 0.05747938166220479 1116 9 0.04334204138704223 1116 10-11 -0.018189487356899292 1116 12-13 -0.02014031327156829 1116 14-15 0.060170281403557624 1116 16-17 -0.10305166036723534 1116 18-19 0.4365202154551824 1116 20-21 0.1495915401934056 1116 22-23 0.05799587517453375 1116 24-25 0.09374907990554959 1116 26-27 0.11942326269321768 1116 28-29 0.03642992317077898 1116 30-31 0.3326822916301353 1116 32-33 0.3382890005745125 1116 34-35 0.20143163409448306 1116 36-37 0.09834432406816518 1116 38-39 0.09771849790543996 1116 40-41 0.6052316605500323 1116 42-43 0.10622065911536538 1116 44-45 0.050331268271229135 1116 46-47 0.1621861484683862 1116 48-49 0.14809638319006524 1116 50-51 0.17305930503233924 1116 52-53 0.6993767403241336 1116 54-55 0.22541058631744448 1116 56-57 -0.13220523126511097 1116 58-59 0.0098319862575309 1116 60-61 -0.006606297618532153 1116 62-63 0.16702457569270734 1116 64-65 0.6077408723192548 1116 66-67 0.23435184346325144 1116 68-69 0.13539547017546738 1116 70-71 0.07048626317033069 1116 72-73 0.14986452576664533 1116 74-75 0.4505217321835815 1116 76 0.3947478790368564 2118 1 0.09124059084800251 2118 2 0.10189677415388587 2118 3 0.0918661404976504 2118 4 0.08382837437200408 2118 5 0.1719951295805231 2118 6 -0.14844042164448012 2118 7 0.21866074544376346 2118 8 0.14049872304515532 2118 9 0.11496659941765586 2118 10-11 0.11794313836936254 2118 12-13 0.14460395009793103 2118 14-15 0.20236532428855014 2118 16-17 0.18669421815926768 2118 18-19 0.680419876038755 2118 20-21 0.24633728657564546 2118 22-23 0.17389392255557823 2118 24-25 0.07044901500609768 2118 26-27 0.22244022938819086 2118 28-29 0.08373180475539499 2118 30-31 0.40262203731593615 2118 32-33 0.4311748474667567 2118 34-35 0.3853259725321436 2118 36-37 0.25314825975245014 2118 38-39 0.21783488747631452 2118 40-41 0.7556213450010105 2118 42-43 0.20548001054866916 2118 44-45 0.19226808962704212 2118 46-47 0.3094830515690603 2118 48-49 0.2869457165672671 2118 50-51 0.31741648666100275 2118 52-53 0.8250537897086332 2118 54-55 0.3055154922577543 2118 56-57 0.07969966210140456 2118 58-59 0.10176862302397893 2118 60-61 0.2185272609355451 2118 62-63 0.3192267649009324 2118 64-65 0.811765930435044 2118 66-67 0.49513620605063835 2118 68-69 0.2172053168457566 2118 70-71 0.32683482100070904 2118 72-73 0.14726742117038327 2118 74-75 0.45029505372056633 2118 76 0.5431045287223881 1119 1 0.11248433183137507 1119 2 0.06804162564179705 1119 3 0.09434153439353565 1119 4 0.057140089166182406 1119 5 0.12806724366978273 1119 6 -0.20562856865426937 1119 7 0.09770080222084232 1119 8 0.00123577726003532 1119 9 0.09038594652852794 1119 10-11 0.07295232855863532 1119 12-13 0.04839174566572524 1119 14-15 0.028724464208664813 1119 16-17 0.006196888201273509 1119 18-19 0.4548920392620204 1119 20-21 0.1505166752040168 1119 22-23 0.09500269105641479 1119 24-25 0.09891330983096935 1119 26-27 0.049096897719472565 1119 28-29 0.10588470015824925 1119 30-31 0.3718549177289816 1119 32-33 0.37425602938821356 1119 34-35 0.20979826984898153 1119 36-37 0.11786387025433243 1119 38-39 0.11368535259114765 1119 40-41 0.6922434652519343 1119 42-43 0.107055939281409 1119 44-45 -0.034520040856037326 1119 46-47 0.22025949875801132 1119 48-49 0.04042243761777087 1119 50-51 0.1763486546707398 1119 52-53 0.5175299507259581 1119 54-55 0.25746307393860235 1119 56-57 0.046183956199257636 1119 58-59 0.20371072545558633 1119 60-61 0.20009381586376662 1119 62-63 0.2808179579962342 1119 64-65 0.7362549058058789 1119 66-67 0.29035981425163016 1119 68-69 0.18151444060693223 1119 70-71 0.17428984050680896 1119 72-73 0.09026186712083728 1119 74-75 0.48508142234688023 1119 76 0.6100903487121485 2119 1 0.14049805521149494 2119 2 0.0363771098285639 2119 3 0.05298722856476701 2119 4 0.03228913830510294 2119 5 0.11535600384516442 2119 6 0.007017541681022976 2119 7 0.023008325773140825 2119 8 0.12482580517498576 2119 9 0.08744128984489663 2119 10-11 0.12907679532354877 2119 12-13 0.055631163404484596 2119 14-15 0.1578265954289293 2119 16-17 0.15311649774393032 2119 18-19 0.6185925128391006 2119 20-21 0.26611773616488676 2119 22-23 0.23517459245446304 2119 24-25 0.08875122749445552 2119 26-27 0.18475596204368827 2119 28-29 0.09269899136685211 2119 30-31 0.43378854686139334 2119 32-33 0.37259985411003527 2119 34-35 0.3429068892787299 2119 36-37 0.189287183983609 2119 38-39 0.13561819122952556 2119 40-41 0.6864864494779184 2119 42-43 0.25979779761081545 2119 44-45 0.24044631868502364 2119 46-47 0.18871436665777708 2119 48-49 0.15164849361814703 2119 50-51 0.20905620853150708 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-0.009236296428518642 2111 5 0.03185124688732799 2111 6 0.13387155851911103 2111 7 -0.05381311230110697 2111 8 0.005540577397354696 2111 9 -0.07328025529750448 2111 10-11 -0.06718865221751003 2111 12-13 0.006412226218223793 2111 14-15 -0.024786936916228797 2111 16-17 0.005293405905938187 2111 18-19 0.5015087386930617 2111 20-21 0.14148195248584017 2111 22-23 -0.030749981150755445 2111 24-25 0.02184501334032518 2111 26-27 -0.12118766994741037 2111 28-29 -0.02078483574035772 2111 30-31 -12.29574585307953 2111 32-33 -10.605353667362792 2111 34-35 -5.510968341312459 2111 36-37 -1.2201139727598402 2111 38-39 -0.312989859124329 2111 40-41 -6.473242493679205 2111 42-43 -0.7888234803067959 2111 44-45 -0.14585772940780117 2111 46-47 -0.15403397480433512 2111 48-49 -0.09661234273563224 2111 50-51 -0.05203942586321375 2111 52-53 0.25890270906020163 2111 54-55 0.007861208060781166 2111 56-57 -0.04504109307259796 2111 58-59 0.03179471752196861 2111 60-61 -0.00896548953151921 2111 62-63 -0.13162224232375763 2111 64-65 0.4017205420549317 2111 66-67 9.696826553735605E-4 2111 68-69 -0.0020744673684092163 2111 70-71 -0.045619246187847295 2111 72-73 -0.05373597531698948 2111 74-75 0.13485326202572878 2111 76 0.06860248280676728 2110 1 0.1354615749442587 2110 2 0.005395079201605313 2110 3 0.020802078780064903 2110 4 0.024732509715768458 2110 5 -0.1239333594674008 2110 6 0.06684385053025466 2110 7 -0.15411832249100144 2110 8 -0.20543195798025948 2110 9 -0.08365310705153206 2110 10-11 -0.042271074716026646 2110 12-13 0.023264495610590075 2110 14-15 0.005020946725963427 2110 16-17 -0.11400584180534423 2110 18-19 0.5296534272197704 2110 20-21 0.040169061936332184 2110 22-23 -0.09336388023955777 2110 24-25 -0.11859125685371907 2110 26-27 -0.14466165478710735 2110 28-29 -0.09758606975153583 2110 30-31 0.0633755223705208 2110 32-33 -0.3062096015679501 2110 34-35 -0.16000389429119366 2110 36-37 -0.14765869134946286 2110 38-39 -0.1649196121073615 2110 40-41 0.2611201366829121 2110 42-43 -0.17247834369946702 2110 44-45 -0.26477066863061083 2110 46-47 -0.26189850557230443 2110 48-49 -0.21062960743962122 2110 50-51 -0.3781557264064901 2110 52-53 0.25922946434089766 2110 54-55 0.02075182914634155 2110 56-57 -0.025362122083308236 2110 58-59 -0.17335727915678945 2110 60-61 -0.4108558028300564 2110 62-63 -0.19933295116440775 2110 64-65 0.38649317334756006 2110 66-67 -0.013889773205825406 2110 68-69 -0.4083299162721872 2110 70-71 -0.13960881006080683 2110 72-73 -0.1824470635230213 2110 74-75 -0.07769084814771077 2110 76 -0.6815440381263329 2109 1 -0.128333069221803 2109 2 0.012613909817758895 2109 3 -0.01267106889240921 2109 4 -0.054941408854809026 2109 5 -0.22363878760933176 2109 6 0.03533689954373642 2109 7 -0.06754235455184698 2109 8 -0.1252472092870036 2109 9 0.024009741384823258 2109 10-11 -0.0611099627338163 2109 12-13 -0.03535377441677667 2109 14-15 -0.018512897168363907 2109 16-17 -0.0685939787335954 2109 18-19 0.4600897737355325 2109 20-21 0.1373055379266077 2109 22-23 -0.09711270620845625 2109 24-25 -0.08432792658126687 2109 26-27 -0.03898833847625127 2109 28-29 -0.008405172716003051 2109 30-31 0.2297337646389792 2109 32-33 0.14397787514086957 2109 34-35 0.09761369259914687 2109 36-37 -0.044691825690634346 2109 38-39 -0.24893487851309715 2109 40-41 0.16145447439622274 2109 42-43 0.04718229172986099 2109 44-45 -0.10974707611170942 2109 46-47 -0.2214837905486533 2109 48-49 -0.08637147257791611 2109 50-51 -0.09906432981124169 2109 52-53 0.18761431056849176 2109 54-55 -0.04372785004130009 2109 56-57 0.04034969664047594 2109 58-59 -0.09955486170439798 2109 60-61 -0.25088924014242764 2109 62-63 -0.004538298074933778 2109 64-65 0.539919545326228 2109 66-67 -0.12052426883526124 2109 68-69 -0.24028203237516976 2109 70-71 -0.25969652298518753 2109 72-73 -0.208096181797508 2109 74-75 -0.02198221352517038 2109 76 -0.5610607912722259 2108 1 -0.1617468068324115 2108 2 -0.0777960753329694 2108 3 -0.0278734187104277 2108 4 -8.294793147101132E-4 2108 5 0.04622372015372633 2108 6 0.03118948124366483 2108 7 0.10314917547120928 2108 8 0.09397480071486797 2108 9 -0.06614072400098081 2108 10-11 -0.0161796806317156 2108 12-13 0.05258651389009117 2108 14-15 -0.010016499744232021 2108 16-17 -0.08085760084158267 2108 18-19 0.558033746853944 2108 20-21 0.06520898095743632 2108 22-23 -0.07678881617186306 2108 24-25 -0.13654619945593538 2108 26-27 -0.10302356274754487 2108 28-29 -0.04845430400978756 2108 30-31 0.3157069626926656 2108 32-33 0.17422580524714704 2108 34-35 0.1288274394235671 2108 36-37 -0.02886899932305198 2108 38-39 0.027065527234981346 2108 40-41 0.3882681118759308 2108 42-43 -0.05462810707663124 2108 44-45 -0.2367411848852825 2108 46-47 -0.0694130521048919 2108 48-49 -0.047592078646012226 2108 50-51 0.09637140891396712 2108 52-53 0.3899074118786672 2108 54-55 -0.10508904824461496 2108 56-57 -0.13776327523775933 2108 58-59 -0.05374972967265279 2108 60-61 -0.19809297501232948 2108 62-63 -0.1854247454715363 2108 64-65 0.3510503097120221 2108 66-67 0.019521817218397075 2108 68-69 0.012715878760467092 2108 70-71 -0.1417185255194724 2108 72-73 -0.16232778430579486 2108 74-75 0.11475041701784505 2108 76 -0.8221789168467097 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 36.0 13 338.0 14 1215.0 15 2374.0 16 3257.0 17 4269.0 18 5981.0 19 7980.0 20 8714.0 21 8377.0 22 8123.0 23 8041.0 24 8131.0 25 8514.0 26 8962.0 27 10224.0 28 11723.0 29 14038.0 30 17204.0 31 24386.0 32 36493.0 33 49740.0 34 77886.0 35 137314.0 36 348457.0 37 1571461.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.76084647825921 2.136546103852782 53.724448114519774 4.378159303368231 2 2.414990691239947 1.8388839726103845 4.558166428125757 91.18795890802392 3 2.795942832422598 50.41915645052805 4.360871905839071 42.42402881121029 4 3.270800788338895 1.320555764654741 90.67722540626433 4.7314180407420325 5 2.6898267441914134 1.7323063276490525 52.69681303469773 42.8810538934618 6 51.121184237082396 2.174016118400211 5.4681465014629245 41.236653143054475 7 2.775592376593367 2.338707951657387 54.26879133817465 40.6169083335746 8 3.907623196834224 3.1476490607949934 90.68645654086728 2.2582712015035002 9 5.571115611988558 2.5403243233263635 89.51783685983655 2.370723204848528 10-11 71.32163412901518 21.781659330012644 3.0251477086435727 3.871558832328608 12-13 21.325137764194025 27.85421436960372 22.25664316503716 28.564004701165096 14-15 3.1958187995412968 46.03424163501856 28.112371440715766 22.657568124724378 16-17 27.15783016306799 3.1503764414731377 46.82130075917691 22.870492636281966 18-19 22.286308674874824 45.664597633724526 4.130030601211209 27.919063090189443 20-21 27.97862488822984 1.6232740400773906 42.35078815007643 28.047312921616342 22-23 21.248792085057353 2.372926089242413 51.9105301650401 24.467751660660138 24-25 3.6927685389505625 27.551768832248886 21.983590399452176 46.771872229348375 26-27 15.196902199066061 34.02172421649696 25.198521843591852 25.582851740845125 28-29 25.689660164171535 4.928523744366385 22.15023335888679 47.23158273257529 30-31 47.60815008482154 6.307676233898489 26.926988858440133 19.15718482283984 32-33 61.13132170126454 12.156816836246806 4.164206779093494 22.54765468339516 34-35 3.2598283260722067 45.768426918156344 28.148121107450823 22.823623648320627 36-37 22.590957096623544 13.60551753307159 38.022309134753165 25.781216235551703 38-39 47.41689775972951 3.3194320838153457 47.20111998838556 2.0625501680695892 40-41 22.719857303442918 42.46628223187015 29.59868901104757 5.215171453639353 42-43 36.6220509147425 2.542799945788064 55.41494159838775 5.420207541081696 44-45 2.8908556800220206 4.945811141895547 86.5318585337014 5.631474644381029 46-47 6.242533795393579 83.7207892284408 5.8488888441318725 4.1877881320337575 48-49 19.155401535473363 23.112788939758037 36.201593713429496 21.530215811339108 50-51 49.5146730982499 19.44446192765392 24.982408394625967 6.058456579470208 52-53 44.92063112428086 24.494102353981283 27.006376616025502 3.578889905712352 54-55 8.518742769818722 59.95424714013156 3.339656660536354 28.18735342951336 56-57 20.437627111674487 27.471793638825144 7.1341564987817 44.956422750718666 58-59 1.7609857844723087 28.576193994811263 41.76068787058285 27.90213235013358 60-61 4.942370446270811 43.82342685731477 29.610269889003998 21.62393280741042 62-63 2.856113046152736 19.76121572364333 62.80356271444031 14.57910851576363 64-65 25.418789303129063 49.0765298822317 22.308924954652053 3.1957558599871856 66-67 21.38832907652149 22.667176896651995 27.674438023211266 28.270056003615245 68-69 3.997563819658876 27.829269326324386 45.778329408003145 22.394837446013597 70-71 40.97329306880258 5.3148886872025844 26.88712714083648 26.82469110315835 72-73 27.347718797820946 65.59363118847921 3.2468417980739654 3.8118082156258772 74-75 23.62192797281347 31.828364675133297 21.247659173083356 23.30204817896988 76 10.424090911570346 80.73483188215701 3.3238378526031167 5.517239353669523 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.5 3 0.5 4 0.0 5 0.5 6 0.5 7 0.5 8 1.0 9 0.5 10 0.0 11 0.0 12 0.0 13 0.5 14 1.0 15 2.0 16 1.5 17 0.0 18 2.0 19 5.0 20 10.0 21 14.0 22 18.5 23 30.0 24 60.5 25 84.0 26 107.0 27 218.5 28 516.5 29 726.0 30 1530.0 31 3571.5 32 19700.0 33 34591.0 34 127831.5 35 157470.5 36 367526.0 37 641183.0 38 371765.0 39 63924.0 40 24012.5 41 69244.5 42 115965.0 43 68813.0 44 18264.0 45 17269.5 46 19672.0 47 47566.5 48 265616.5 49 279810.5 50 103849.0 51 140445.5 52 109861.0 53 75900.0 54 109120.0 55 64811.5 56 13304.5 57 4831.5 58 3557.0 59 3259.5 60 3043.5 61 3354.5 62 3584.0 63 3804.0 64 4313.5 65 4951.0 66 5645.0 67 5991.0 68 6013.0 69 6026.0 70 5780.0 71 5543.0 72 5281.0 73 4572.5 74 3727.0 75 3328.0 76 2897.5 77 2113.5 78 1500.5 79 1241.0 80 995.0 81 632.5 82 408.5 83 301.0 84 236.5 85 136.5 86 89.0 87 77.0 88 66.0 89 45.0 90 39.0 91 36.5 92 30.0 93 30.0 94 24.0 95 18.0 96 18.0 97 17.0 98 20.5 99 65.0 100 105.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 2383239.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 7.684597992645234 #Duplication Level Percentage of deduplicated Percentage of total 1 90.9658695377928 6.990361384493507 2 4.240702918616153 0.6517619427160494 3 1.6167093922206826 0.3727128525044926 4 0.7419570210167514 0.22806565737337461 5 0.4318135994964135 0.1659156959943526 6 0.22374939435918037 0.10316544880488866 7 0.2030470784095391 0.10922346198108965 8 0.10973767866323672 0.06746319561384452 9 0.1144790892616529 0.07917532015859571 >10 0.9055648845687698 1.455056663379244 >50 0.15579008771703043 0.8543985609619501 >100 0.20125592551883184 3.5719221017260114 >500 0.037962441973343745 2.0878150890932496 >1k 0.041870339988283406 6.423894577383543 >5k 0.00446616959875023 2.5165237415668784 >10k+ 0.005024440798594008 74.32254430624896 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TCATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTG 619157 25.979643669812386 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAA 470333 19.735032869133143 No Hit TCATTGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTG 219656 9.216700465207223 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAA 170496 7.153961478475301 No Hit TCATTGTTTGGCATAGTCACGTTCAGCACTGGATATGTGCTTCCTGATTT 110229 4.625176073402626 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCAC 103320 4.3352764871672544 No Hit TCATTGTTTGGCATAGTCACGTTCAATGCTAGGTATGTGTAGTTTAAGTG 16118 0.6763064887743109 No Hit CTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAATG 12050 0.5056144180252169 No Hit ATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGG 11065 0.46428411082564525 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCATCCAGCACACTGGAGTTTAACAA 9307 0.39051895340752646 No Hit GCCTCCCTTAGGTCACTAATTGCCTCATCCGGCACACTGGAGTTTAACAA 8811 0.36970694084814826 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGTAGTTTAACAA 8617 0.361566758516456 No Hit TCATTGTTTGGCATAGTCGCGTTCAATGCTGGGTATGTGTAGTTTAAGTG 7872 0.3303067799746479 No Hit TTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTCAAC 6320 0.2651853213211096 No Hit TGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTCAACC 6160 0.25847176888260054 No Hit TCATTGTTTGGCATAGTCACGTTCAGTGCTAGATATTTGTATTTTAATTG 5847 0.2453383819247671 No Hit CATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGG 5725 0.2402192981904039 No Hit CTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAATG 4771 0.20018974177579335 No Hit CCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAAT 4677 0.19624552971816925 No Hit TGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTC 4354 0.182692545732929 No Hit TCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAATGA 4289 0.1799651650547847 No Hit TCATTGTTTGGCATAGTCACTCAAAACGGAACAAGTTCTGCTTGCATAAG 4166 0.1748041216176808 No Hit TCATTGTTTGGCATAGTCACGTTCAGTGCTGGGTATGTGTAGTTTAAGTG 4092 0.17169910361487037 No Hit TCATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTATTTTAAGTG 3845 0.16133505703792192 No Hit TTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGT 3787 0.15890139427896238 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCATCCAGCACCCTGGAGTTTAACAA 3549 0.14891498502668007 No Hit TCATTGTTTGGCATAGTCGCGTTCAGTGCTGGATATTTGTATTTTAATTG 3332 0.13980972953195211 No Hit CCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAATGAA 3145 0.1319632651194446 No Hit CTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCACTG 3089 0.1296135217659664 No Hit GCCTCCCTTAGGTCACTAATTGCCTCATCCGGCACCCTGGAGTTTAACAA 3073 0.1289421665221155 No Hit TCATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGGAGTTTAAGTG 3051 0.1280190530618205 No Hit TCATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGCTTAAGTG 2833 0.11887183786435183 No Hit TCATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAATTG 2711 0.11375275412998864 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0012587910822204572 0.0 0.0 2 0.0012587910822204572 0.0 0.0 3 0.0015525090014052304 0.0 0.0 4 0.0015525090014052304 0.0 0.0 5 0.0015525090014052304 0.0 0.0 6 0.0015525090014052304 0.0 0.0 7 0.0015525090014052304 0.0 0.0 8 0.0015525090014052304 0.0 0.0 9 0.0015525090014052304 0.0 0.0 10 0.0015525090014052304 0.0 0.0 11 0.0015525090014052304 0.0 0.0 12 0.0015525090014052304 0.0 0.0 13 0.0015525090014052304 0.0 0.0 14 0.0015525090014052304 0.0 0.0 15 0.0015525090014052304 0.0 0.0 16 0.0015525090014052304 0.0 0.0 17 0.0015525090014052304 0.0 0.0 18 0.0015525090014052304 0.0 0.0 19 0.0015525090014052304 0.0 0.0 20 0.0015525090014052304 0.0 0.0 21 0.0015525090014052304 0.0 0.0 22 0.0015525090014052304 0.0 0.0 23 0.0015525090014052304 0.0 0.0 24 0.0015525090014052304 0.0 0.0 25 0.0015944687041459123 0.0 0.0 26 0.0015944687041459123 0.0 0.0 27 0.0015944687041459123 0.0 0.0 28 0.0016364284068865942 0.0 0.0 29 0.0017203478123679582 0.0 0.0 30 0.0017203478123679582 0.0 0.0 31 0.0017203478123679582 0.0 0.0 32 0.00176230751510864 0.0 0.0 33 0.00176230751510864 0.0 0.0 34 0.00176230751510864 0.0 0.0 35 0.00176230751510864 0.0 0.0 36 0.001804267217849322 0.0 0.0 37 0.0018462269205900038 0.0 0.0 38 0.0019301463260713676 0.0 0.0 39 0.002097985137034095 0.0 0.0 40 0.002181904542515459 0.0 0.0 41 0.0022658239479968227 0.0 0.0 42 0.0023917030562188683 0.0 0.0 43 0.0025175821644409144 0.0 0.0 44 0.0025175821644409144 0.0 0.0 45 0.00264346127266296 0.0 0.0 46 0.0027693403808850057 0.0 0.0 47 0.0029371791918477333 0.0 0.0 48 0.0029371791918477333 0.0 0.0 49 0.0031889374082918246 0.0 0.0 50 0.0032308971110325065 0.0 0.0 51 0.003398735921995234 0.0 0.0 52 0.003566574732957962 0.0 0.0 53 0.0038183329494020533 0.0 0.0 54 0.003902252354883417 0.0 0.0 55 0.004279889679549554 0.0 0.0 56 0.004909285220659782 0.0 0.0 57 0.005119083734363193 0.0 0.0 58 0.00528692254532592 0.0 0.0 59 0.005664559869992057 0.0 0.0 60 0.006042197194658194 0.0 0.0 61 0.006377874816583649 0.0 0.0 62 0.006755512141249786 0.0 0.0 63 0.007175109168656606 4.1959702740681906E-5 0.0 64 0.007636665898804107 4.1959702740681906E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATAGTAC 45 5.456968E-12 70.00001 13 TGTACAA 35 3.7161954E-9 70.00001 50 TTCTAAG 25 2.8419308E-6 70.0 42 CTAAGTT 105 0.0 70.0 44 CTAAGGT 30 1.02476406E-7 70.0 7 TGTTGTA 15 0.002225198 70.0 38 TAGTTAA 25 2.8419308E-6 70.0 40 AGTGCTA 755 0.0 70.0 25 GTGCTAG 765 0.0 70.0 26 AGTGAAC 15 0.002225198 70.0 49 GCCCTAG 20 7.930361E-5 70.0 10 AGATTTT 20 7.930361E-5 70.0 39 AAACAGT 20 7.930361E-5 70.0 55 ACATGTG 15 0.002225198 70.0 33 GTGAGTG 15 0.002225198 70.0 44 GACATGT 15 0.002225198 70.0 32 TTAAAGT 15 0.002225198 70.0 43 AACAGTT 20 7.930361E-5 70.0 56 GTAGTGA 20 7.930361E-5 70.0 13 TTGGGAC 15 0.002225198 70.0 48 >>END_MODULE