FastQCFastQC Report
Tue 25 Aug 2015
000000000-AHV5L l01n02 kokuya2.34200000033ce9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AHV5L l01n02 kokuya2.34200000033ce9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2383239
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTG61915725.979643669812386No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAA47033319.735032869133143No Hit
TCATTGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTG2196569.216700465207223No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAA1704967.153961478475301No Hit
TCATTGTTTGGCATAGTCACGTTCAGCACTGGATATGTGCTTCCTGATTT1102294.625176073402626No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCAC1033204.3352764871672544No Hit
TCATTGTTTGGCATAGTCACGTTCAATGCTAGGTATGTGTAGTTTAAGTG161180.6763064887743109No Hit
CTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAATG120500.5056144180252169No Hit
ATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGG110650.46428411082564525No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCATCCAGCACACTGGAGTTTAACAA93070.39051895340752646No Hit
GCCTCCCTTAGGTCACTAATTGCCTCATCCGGCACACTGGAGTTTAACAA88110.36970694084814826No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGTAGTTTAACAA86170.361566758516456No Hit
TCATTGTTTGGCATAGTCGCGTTCAATGCTGGGTATGTGTAGTTTAAGTG78720.3303067799746479No Hit
TTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTCAAC63200.2651853213211096No Hit
TGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTCAACC61600.25847176888260054No Hit
TCATTGTTTGGCATAGTCACGTTCAGTGCTAGATATTTGTATTTTAATTG58470.2453383819247671No Hit
CATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGG57250.2402192981904039No Hit
CTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAATG47710.20018974177579335No Hit
CCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAAT46770.19624552971816925No Hit
TGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTC43540.182692545732929No Hit
TCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAATGA42890.1799651650547847No Hit
TCATTGTTTGGCATAGTCACTCAAAACGGAACAAGTTCTGCTTGCATAAG41660.1748041216176808No Hit
TCATTGTTTGGCATAGTCACGTTCAGTGCTGGGTATGTGTAGTTTAAGTG40920.17169910361487037No Hit
TCATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTATTTTAAGTG38450.16133505703792192No Hit
TTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGT37870.15890139427896238No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCATCCAGCACCCTGGAGTTTAACAA35490.14891498502668007No Hit
TCATTGTTTGGCATAGTCGCGTTCAGTGCTGGATATTTGTATTTTAATTG33320.13980972953195211No Hit
CCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAATGAA31450.1319632651194446No Hit
CTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCACTG30890.1296135217659664No Hit
GCCTCCCTTAGGTCACTAATTGCCTCATCCGGCACCCTGGAGTTTAACAA30730.1289421665221155No Hit
TCATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGGAGTTTAAGTG30510.1280190530618205No Hit
TCATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGCTTAAGTG28330.11887183786435183No Hit
TCATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAATTG27110.11375275412998864No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGTAC455.456968E-1270.0000113
TGTACAA353.7161954E-970.0000150
TTCTAAG252.8419308E-670.042
CTAAGTT1050.070.044
CTAAGGT301.02476406E-770.07
TGTTGTA150.00222519870.038
TAGTTAA252.8419308E-670.040
AGTGCTA7550.070.025
GTGCTAG7650.070.026
AGTGAAC150.00222519870.049
GCCCTAG207.930361E-570.010
AGATTTT207.930361E-570.039
AAACAGT207.930361E-570.055
ACATGTG150.00222519870.033
GTGAGTG150.00222519870.044
GACATGT150.00222519870.032
TTAAAGT150.00222519870.043
AACAGTT207.930361E-570.056
GTAGTGA207.930361E-570.013
TTGGGAC150.00222519870.048
Produced by FastQC (version 0.11.2)