FastQCFastQC Report
Tue 25 Aug 2015
000000000-AHV5L l01n02 kokuya1.34200000033cdc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AHV5L l01n02 kokuya1.34200000033cdc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2044449
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTG47951423.454436867830893No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAA43426121.24097984346883No Hit
TCATTGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTG21725110.626383930340156No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAA20914410.22984677045013No Hit
TCATTGTTTGGCATAGTCACGTTCAGCACTGGATATGTGCTTCCTGATTT386961.8927349129276398No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCAC352381.7235939854699238No Hit
TCATTGTTTGGCATAGTCACGTTCAATGCTAGGTATGTGTAGTTTAAGTG126080.6166942780181849No Hit
ATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGG111720.5464553040941593No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCATCCAGCACACTGGAGTTTAACAA83750.4096458263326696No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGTAGTTTAACAA79780.3902273913411389No Hit
TGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTCAACC79060.3867056600580401No Hit
GCCTCCCTTAGGTCACTAATTGCCTCATCCGGCACACTGGAGTTTAACAA78860.3857274013682904No Hit
CATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGG60120.2940645621387474No Hit
TCATTGTTTGGCATAGTCACGTTCAGTGCTAGATATTTGTATTTTAATTG57740.28242328373072645No Hit
TCATTGTTTGGCATAGTCACTCAAAACGGAACAAGTTCTGCTTGCATAAG50180.24544510525818936No Hit
CTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAATG49200.24065163767841602No Hit
TGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTC47060.2301842696980947No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCATCCAGCACCCTGGAGTTTAACAA40530.19824412347776835No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGTAGTTTAACAA37730.18454850182127314No Hit
CTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAATG36810.18004851184842469No Hit
TTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTCAAC35810.17515721839967638No Hit
CCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAAT33680.16473876335384252No Hit
TCATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTATTTTAAGTG33280.1627822459743432No Hit
TCATTGTTTGGCATAGTCGCGTTCAGTGCTGGATATTTGTATTTTAATTG32740.16014094751201913No Hit
TTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGT32690.1598963828395817No Hit
TCATTGTTTGGCATAGTCACGTTCAGTGCTGGGTATGTGTAGTTTAAGTG31920.15613008688404553No Hit
CATTGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTGG26740.13079318681952937No Hit
TCATTGTTTGGCATAGTCACGTTCAGTGCTGGGTATTTGTATTTTAATTG23750.11616821940777196No Hit
TCATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGGAGTTTAAGTG23620.1155323512594347No Hit
TCATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAATTG23140.11318453040403552No Hit
ATTGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTGGT22450.1098095379243992No Hit
TCATTGTTTGGACTGGAGTCACTCAAAACGGAACAAGTTCTGCTTGCATA22410.10961388618644925No Hit
TCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAATGA21740.1063367195757879No Hit
CCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAAT21230.10384215991692627No Hit
TCATTGTTTGGCATAGTCACGTCCAATGCTGGGTATGTGTAGTTTAAGTG20820.10183672960293946No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCAAG150.00222515370.021
GTTCTAA150.00222515370.013
GTACACT500.070.032
ATATGTC207.930092E-570.033
CGCCTTA150.00222515370.021
TAGTTAT207.930092E-570.030
CCCGTAG401.3460522E-1070.05
TATCTAG150.00222515370.053
CACAGTA150.00222515370.046
GAGTTAA600.070.040
TAGGCTA150.00222515370.09
ATAGTCT700.070.013
AGCTAAG150.00222515370.065
GCTAAGA150.00222515370.066
AAGTGTA150.00222515370.035
GTAACAC150.00222515370.042
AGGCTAC150.00222515370.010
TCATTGT887550.069.846211
GCTAGGT15500.069.7741928
TAGGTAT15300.069.7712430
Produced by FastQC (version 0.11.2)