##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AHV5L l01n02 kokuya1.34200000033cdc.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2044449 Sequences flagged as poor quality 0 Sequence length 76 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.789501229915736 34.0 34.0 34.0 32.0 34.0 2 33.24005245423094 34.0 34.0 34.0 34.0 34.0 3 33.167797289147344 34.0 34.0 34.0 34.0 34.0 4 33.319934613189176 34.0 34.0 34.0 34.0 34.0 5 33.38704120278862 34.0 34.0 34.0 34.0 34.0 6 36.895101320698146 38.0 38.0 38.0 36.0 38.0 7 36.98661595373619 38.0 38.0 38.0 36.0 38.0 8 37.1000748857027 38.0 38.0 38.0 37.0 38.0 9 37.15399748294039 38.0 38.0 38.0 37.0 38.0 10-11 36.992917651650885 38.0 38.0 38.0 37.0 38.0 12-13 36.94975590000044 38.0 38.0 38.0 36.5 38.0 14-15 36.826218213318114 38.0 38.0 38.0 36.0 38.0 16-17 36.974174948849296 38.0 38.0 38.0 37.0 38.0 18-19 36.229287940173606 38.0 38.0 38.0 33.5 38.0 20-21 36.72520126449719 38.0 38.0 38.0 35.0 38.0 22-23 36.706569838621554 38.0 37.5 38.0 36.0 38.0 24-25 36.86801235932029 38.0 38.0 38.0 36.5 38.0 26-27 36.43438574403177 38.0 38.0 38.0 34.5 38.0 28-29 36.82165830500052 38.0 38.0 38.0 36.5 38.0 30-31 36.422472998837335 38.0 38.0 38.0 35.0 38.0 32-33 36.09140384524143 38.0 38.0 38.0 32.5 38.0 34-35 36.225805583802774 38.0 37.0 38.0 34.0 38.0 36-37 36.35355907630858 38.0 37.5 38.0 34.0 38.0 38-39 36.77368254233781 38.0 38.0 38.0 36.0 38.0 40-41 35.689089089529745 38.0 37.0 38.0 31.0 38.0 42-43 36.13884034280141 38.0 37.0 38.0 34.0 38.0 44-45 36.34592401179975 38.0 37.0 38.0 34.0 38.0 46-47 36.13738811777648 38.0 37.0 38.0 34.0 38.0 48-49 36.30840681278917 38.0 37.5 38.0 34.0 38.0 50-51 36.29121954130429 38.0 37.5 38.0 34.0 38.0 52-53 35.603567024660435 38.0 37.0 38.0 30.0 38.0 54-55 36.16118572779267 38.0 37.0 38.0 34.0 38.0 56-57 36.52778205765955 38.0 37.5 38.0 34.5 38.0 58-59 36.43171778802015 38.0 37.5 38.0 34.5 38.0 60-61 36.42983659655975 38.0 38.0 38.0 34.0 38.0 62-63 36.177992456647246 38.0 37.0 38.0 34.0 38.0 64-65 35.7509649299151 38.0 37.0 38.0 30.5 38.0 66-67 36.26837010852313 38.0 37.0 38.0 34.0 38.0 68-69 36.488841003126026 38.0 38.0 38.0 35.0 38.0 70-71 36.463285217679676 38.0 38.0 38.0 34.5 38.0 72-73 36.04605054956127 38.0 37.0 38.0 33.5 38.0 74-75 36.06905307982738 38.0 37.0 38.0 33.0 38.0 76 34.06147035215845 37.0 34.0 38.0 24.0 38.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2168453762121203 1101 2 0.05290524286213838 1101 3 -0.02011493188279445 1101 4 0.02908492801620355 1101 5 -0.005257402085007357 1101 6 0.03442508964873525 1101 7 0.12932512902970217 1101 8 0.07626937746453422 1101 9 0.06077494517151649 1101 10-11 0.10724790527726924 1101 12-13 0.13057947122244684 1101 14-15 0.16655561904525484 1101 16-17 0.12138283293221264 1101 18-19 0.6600722687352771 1101 20-21 0.17112777664561918 1101 22-23 0.1746300515643 1101 24-25 0.16745780014941403 1101 26-27 0.1741450556437769 1101 28-29 0.12068429157844207 1101 30-31 0.5181911220233388 1101 32-33 0.4558339830401579 1101 34-35 0.40533458749610674 1101 36-37 0.23522929133586956 1101 38-39 0.14570377984576055 1101 40-41 0.7396018685887711 1101 42-43 0.31343922795438317 1101 44-45 0.1571087401590887 1101 46-47 0.2160128590804149 1101 48-49 0.1638158268739005 1101 50-51 0.19062699656281978 1101 52-53 0.7575644748263031 1101 54-55 0.40037610019847136 1101 56-57 0.15243281824673716 1101 58-59 0.21453962610164012 1101 60-61 0.25125292458496773 1101 62-63 0.31103569543034126 1101 64-65 0.7207668497663562 1101 66-67 0.3303003064270271 1101 68-69 0.2546011262453547 1101 70-71 0.28673938143345623 1101 72-73 0.22911722489248376 1101 74-75 0.5253071324454481 1101 76 0.4993465585771588 1102 1 0.08278706477737785 1102 2 0.05286707324221851 1102 3 0.23884465236196206 1102 4 0.10505935437085157 1102 5 0.07768885215402577 1102 6 0.1237794358471902 1102 7 0.20665971510953085 1102 8 0.12284292004567732 1102 9 0.10194528444071693 1102 10-11 0.15352563330404934 1102 12-13 0.14741447570003885 1102 14-15 0.18808328467939361 1102 16-17 0.20145608924121206 1102 18-19 0.779382059242792 1102 20-21 0.33646058637006604 1102 22-23 0.2348661159828751 1102 24-25 0.18155119827323318 1102 26-27 0.2665973803204338 1102 28-29 0.22325486867373456 1102 30-31 0.5581892135423416 1102 32-33 0.6309818903173436 1102 34-35 0.5003802442337744 1102 36-37 0.37745406635531964 1102 38-39 0.24927630946391588 1102 40-41 0.8506317876985392 1102 42-43 0.3491637901048037 1102 44-45 0.310477209116911 1102 46-47 0.37304047333235957 1102 48-49 0.3256047341951316 1102 50-51 0.41482616857567933 1102 52-53 0.7008073510539674 1102 54-55 0.4196168917776717 1102 56-57 0.30062893669000346 1102 58-59 0.2176284291904409 1102 60-61 0.35634416465719454 1102 62-63 0.4200503320115203 1102 64-65 0.8627967395148772 1102 66-67 0.4724215830540075 1102 68-69 0.24299609372776843 1102 70-71 0.3638599974323995 1102 72-73 0.346004345581882 1102 74-75 0.5957627455816734 1102 76 0.7702216705169036 1103 1 0.2540654860558007 1103 2 0.09484032728443736 1103 3 0.13337267792446994 1103 4 0.16572741159063753 1103 5 0.06817761678072287 1103 6 0.07195914264758585 1103 7 0.29681489491988344 1103 8 0.13127425778736068 1103 9 0.2015088874525972 1103 10-11 0.24725926841699675 1103 12-13 0.22734243321420422 1103 14-15 0.2508001887756066 1103 16-17 0.25852646010469016 1103 18-19 0.7755439847150569 1103 20-21 0.35464539679625773 1103 22-23 0.3195556084309814 1103 24-25 0.1883393904062487 1103 26-27 0.2678618221021196 1103 28-29 0.22741950128664712 1103 30-31 0.5341097153249166 1103 32-33 0.5419705883750083 1103 34-35 0.49861394591575703 1103 36-37 0.27835422760031037 1103 38-39 0.20044570546090057 1103 40-41 0.81906882079074 1103 42-43 0.4187125260328486 1103 44-45 0.34853102648106926 1103 46-47 0.3539824991019813 1103 48-49 0.3065903996635555 1103 50-51 0.28333309915157656 1103 52-53 0.749470422992907 1103 54-55 0.4500089833766552 1103 56-57 0.2727542639877427 1103 58-59 0.38666606288324346 1103 60-61 0.2950625628583481 1103 62-63 0.29481881971103974 1103 64-65 0.7549349368254923 1103 66-67 0.5954045413817894 1103 68-69 0.43715724995789174 1103 70-71 0.3631747062298629 1103 72-73 0.4347183058475679 1103 74-75 0.6659642278521147 1103 76 0.8456236735051164 2116 1 0.05604270505429554 2116 2 0.0915469385178298 2116 3 0.06014850664453775 2116 4 0.08926209814806896 2116 5 0.14744942510608894 2116 6 -0.006563797687533679 2116 7 0.19521822388080778 2116 8 0.10316150887490494 2116 9 0.0813591138545462 2116 10-11 0.12111317801925736 2116 12-13 0.14218120982847893 2116 14-15 0.15716538142105918 2116 16-17 0.09145089891687519 2116 18-19 0.7295771631612951 2116 20-21 0.3292350201019474 2116 22-23 0.2397704123765365 2116 24-25 0.12874973004623058 2116 26-27 0.19222115275272245 2116 28-29 0.12508081730819498 2116 30-31 0.547052607192974 2116 32-33 0.5622748785252298 2116 34-35 0.389800944292773 2116 36-37 0.16262336801398902 2116 38-39 0.11672774819194842 2116 40-41 0.7780137358251622 2116 42-43 0.3515552600494658 2116 44-45 0.19424699022711422 2116 46-47 0.16658412059941696 2116 48-49 0.14413736602563176 2116 50-51 0.2072049801034268 2116 52-53 0.6219136742879456 2116 54-55 0.4098419054069353 2116 56-57 0.233003491404979 2116 58-59 0.23842744041498065 2116 60-61 0.1443224026354386 2116 62-63 0.21222675823049286 2116 64-65 0.780641933423901 2116 66-67 0.42578371833104 2116 68-69 0.3820424556464701 2116 70-71 0.27561421503767747 2116 72-73 0.17054076373531757 2116 74-75 0.5082650793605978 2116 76 0.8192739695692026 1117 1 0.27855999789866814 1117 2 0.1591895507085468 1117 3 0.10551455326063319 1117 4 0.12592847801294482 1117 5 0.10713988030522614 1117 6 0.11912641689607284 1117 7 -0.5673440856706975 1117 8 0.05160832818717154 1117 9 0.06779540046414212 1117 10-11 0.206509286677651 1117 12-13 0.14486735070924084 1117 14-15 -0.50201892448694 1117 16-17 -0.11778233090709023 1117 18-19 0.07333328330666689 1117 20-21 0.21045693354665218 1117 22-23 0.2787402203422502 1117 24-25 0.28733372628279596 1117 26-27 0.3485216294023985 1117 28-29 0.20648764341430592 1117 30-31 0.5418401150008734 1117 32-33 0.5409626001680721 1117 34-35 0.45628308939138407 1117 36-37 0.3168873657106346 1117 38-39 0.24325067501540332 1117 40-41 0.8766795301674222 1117 42-43 0.4448073353583979 1117 44-45 0.24470276296984395 1117 46-47 0.22637056128116484 1117 48-49 0.2779044032625677 1117 50-51 0.17936920876862672 1117 52-53 0.8664041932539348 1117 54-55 0.4938991339108654 1117 56-57 0.2830303651052901 1117 58-59 0.3144914843723825 1117 60-61 0.37556200839843257 1117 62-63 0.2563939174885519 1117 64-65 0.8859745657689615 1117 66-67 0.6304040765650925 1117 68-69 0.41263202701188817 1117 70-71 0.34638453112677325 1117 72-73 0.3807608896971786 1117 74-75 0.4950255637353038 1117 76 0.8113962320554222 2117 1 0.08181016327193902 2117 2 0.018686354812032846 2117 3 0.03424651066022477 2117 4 0.19040342005318678 2117 5 0.15750377182095576 2117 6 -0.061361561661676944 2117 7 0.17566812798686726 2117 8 0.1618265180144789 2117 9 0.10253920226507773 2117 10-11 0.1475130455261322 2117 12-13 0.10500216827716713 2117 14-15 0.029068252514250048 2117 16-17 0.08708394204666092 2117 18-19 0.4908908079079879 2117 20-21 0.271021264535392 2117 22-23 0.18623705805149626 2117 24-25 0.14605480363307066 2117 26-27 0.1998008633125039 2117 28-29 0.14185714520412773 2117 30-31 0.5164201161178568 2117 32-33 0.4582440616070045 2117 34-35 0.378661707927499 2117 36-37 0.2530358141886566 2117 38-39 0.17921767830592472 2117 40-41 0.6642395629870705 2117 42-43 0.30367330978345564 2117 44-45 0.24673389426365588 2117 46-47 0.23364444995113587 2117 48-49 0.26515470054214063 2117 50-51 0.2074791672540428 2117 52-53 0.9026764093712316 2117 54-55 0.3546129253994934 2117 56-57 0.20784288439020315 2117 58-59 0.23261573603821262 2117 60-61 0.24418786486876343 2117 62-63 0.2269079364378328 2117 64-65 0.7802679675040736 2117 66-67 0.4198597628389038 2117 68-69 0.23695144841251192 2117 70-71 0.2732354728226696 2117 72-73 0.23878494364789304 2117 74-75 0.4088964663263823 2117 76 0.5521204043183872 1116 1 0.14151314032955042 1116 2 -0.028246767647779336 1116 3 0.003439571969316546 1116 4 -0.015916821455142838 1116 5 0.04059067687907714 1116 6 -0.05999515155280477 1116 7 0.09078383896007836 1116 8 0.1347883208565861 1116 9 0.08892124753108988 1116 10-11 0.027983305193323815 1116 12-13 0.061622076123441616 1116 14-15 0.13609823231170992 1116 16-17 -0.0337330977319823 1116 18-19 0.4891504100911135 1116 20-21 0.2502685134312941 1116 22-23 0.1611202774388758 1116 24-25 0.19505280080773701 1116 26-27 0.09474315297548941 1116 28-29 0.10424420751652974 1116 30-31 0.4311294082397623 1116 32-33 0.3556349761288402 1116 34-35 0.22138013121797684 1116 36-37 0.22154649776839364 1116 38-39 0.21655151328015876 1116 40-41 0.6749757789881912 1116 42-43 0.09780348243590709 1116 44-45 0.08042710175189427 1116 46-47 0.19768805608689632 1116 48-49 0.21193065329798344 1116 50-51 0.22162495539168248 1116 52-53 0.7435350058726868 1116 54-55 0.30137935472436794 1116 56-57 -0.023719505058480195 1116 58-59 0.10949849352080321 1116 60-61 0.05523775474075876 1116 62-63 0.27255750999219686 1116 64-65 0.6799154755402554 1116 66-67 0.3140713773726347 1116 68-69 0.21255080039267682 1116 70-71 0.21083422096583604 1116 72-73 0.1376419149887269 1116 74-75 0.43215183903982535 1116 76 0.54075509032333 2118 1 0.08371351390788107 2118 2 0.07119636656196349 2118 3 0.12280319193762068 2118 4 0.03506084447933944 2118 5 0.07077718312925185 2118 6 -0.1281650819551814 2118 7 0.20437151337539916 2118 8 0.12568312582818209 2118 9 0.10674557302014165 2118 10-11 0.05852963151592405 2118 12-13 0.09217991639593492 2118 14-15 0.16738516147839988 2118 16-17 0.14383761455166422 2118 18-19 0.615731522139825 2118 20-21 0.26252542379332766 2118 22-23 0.13904682925797118 2118 24-25 0.051958418365202874 2118 26-27 0.26073867211170665 2118 28-29 0.144275725766434 2118 30-31 0.40303288493358735 2118 32-33 0.4111046177174842 2118 34-35 0.359198145600395 2118 36-37 0.28879213213549093 2118 38-39 0.1647884863058593 2118 40-41 0.748883750289437 2118 42-43 0.261417313621223 2118 44-45 0.19353323719586513 2118 46-47 0.30872034639282475 2118 48-49 0.21056957648706032 2118 50-51 0.3171319270335218 2118 52-53 0.744684628079888 2118 54-55 0.2992114789954812 2118 56-57 0.04361082080676937 2118 58-59 0.14468865170346845 2118 60-61 0.2120231008672704 2118 62-63 0.2830267425614821 2118 64-65 0.7825899906512674 2118 66-67 0.34730425545291865 2118 68-69 0.13632885413160523 2118 70-71 0.20439223910143767 2118 72-73 0.1282344330851828 2118 74-75 0.41810891401113537 2118 76 0.5473630375512784 1119 1 0.002041856090997385 1119 2 0.014615036460774888 1119 3 0.018190902296446154 1119 4 0.03555153045126502 1119 5 0.017392696269631358 1119 6 -0.28790155671615736 1119 7 0.05829831233636895 1119 8 0.0061641695433962695 1119 9 0.0883966841274102 1119 10-11 0.006145860188986774 1119 12-13 -0.0212706282791828 1119 14-15 -0.04314127375104704 1119 16-17 -0.03204452514175671 1119 18-19 0.4020993062247413 1119 20-21 0.1375572308490689 1119 22-23 0.07253492571050657 1119 24-25 0.11218779802747747 1119 26-27 0.01330009467021398 1119 28-29 0.10853806719222803 1119 30-31 0.2876284216580203 1119 32-33 0.28778225079417297 1119 34-35 0.2277403046820652 1119 36-37 0.049336873532247694 1119 38-39 0.09019319596903586 1119 40-41 0.6294775775737449 1119 42-43 0.038018926754055826 1119 44-45 -0.06531712108918697 1119 46-47 0.18054497201333675 1119 48-49 0.042650367417238044 1119 50-51 0.17877397222936509 1119 52-53 0.4506783597485011 1119 54-55 0.1790540762148467 1119 56-57 0.01652864376617913 1119 58-59 0.17511654917855424 1119 60-61 0.2026283124119317 1119 62-63 0.2714744775533049 1119 64-65 0.7195803573279065 1119 66-67 0.21788919837455722 1119 68-69 0.20417931961687685 1119 70-71 0.1788978212937522 1119 72-73 0.07078174094750977 1119 74-75 0.4148993191300079 1119 76 0.46485877691871735 2119 1 0.07361043354164565 2119 2 -6.376709141591164E-4 2119 3 0.043164566409096494 2119 4 0.03518834949321814 2119 5 0.07387081741157431 2119 6 -0.0600133805938583 2119 7 0.019448092749208 2119 8 0.169017132577153 2119 9 0.0796990198284746 2119 10-11 0.12442290529528321 2119 12-13 0.049603194605936096 2119 14-15 0.13285900782223337 2119 16-17 0.07869527400549714 2119 18-19 0.5926826836297963 2119 20-21 0.22017457733447543 2119 22-23 0.18777071868857576 2119 24-25 0.04173069216649594 2119 26-27 0.1457313935044624 2119 28-29 0.07012785049651882 2119 30-31 0.3870876645330483 2119 32-33 0.3909517680509964 2119 34-35 0.3257601095265059 2119 36-37 0.13659019691122865 2119 38-39 0.15423615253703815 2119 40-41 0.6309708195293311 2119 42-43 0.2235429137688314 2119 44-45 0.13558827057481437 2119 46-47 0.1999594924516117 2119 48-49 0.10842237106183461 2119 50-51 0.17798685991404994 2119 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0.07221828997466417 2111 7 -0.10329345681968505 2111 8 6.547236059830652E-4 2111 9 -0.053571025866013144 2111 10-11 -0.0498410450626281 2111 12-13 -0.031635952751585705 2111 14-15 -0.018886769596051067 2111 16-17 0.00989524812947451 2111 18-19 0.5173991980084338 2111 20-21 0.12709558523859243 2111 22-23 -0.02418986822281255 2111 24-25 -0.015798311202026127 2111 26-27 -0.12686466014758935 2111 28-29 -0.00756877711997106 2111 30-31 -12.130519907352408 2111 32-33 -10.535979676195122 2111 34-35 -5.381636837783304 2111 36-37 -1.1751619897038452 2111 38-39 -0.2547905305310678 2111 40-41 -6.482191771921432 2111 42-43 -0.7852029450104325 2111 44-45 -0.15987687036890463 2111 46-47 -0.1372106110205138 2111 48-49 -0.04973253440560654 2111 50-51 -0.007322058662374786 2111 52-53 0.24603294059092917 2111 54-55 -0.03118802699132317 2111 56-57 -0.07557509610086299 2111 58-59 -0.04052297596695098 2111 60-61 0.01743073143238405 2111 62-63 -0.17763162944289945 2111 64-65 0.38199570557013374 2111 66-67 -0.024194661466431455 2111 68-69 0.04059421093666771 2111 70-71 -0.04245718568119372 2111 72-73 -0.019273032270348267 2111 74-75 0.12575968396183868 2111 76 -0.2064926789519319 2110 1 0.16007876912885877 2110 2 -0.09168843310649777 2110 3 -0.07777897565100034 2110 4 -0.00788841915850469 2110 5 -0.10401829926233574 2110 6 0.05055983960659205 2110 7 -0.1802074312891122 2110 8 -0.2551613477808843 2110 9 -0.10941127174621101 2110 10-11 -0.09648682434168876 2110 12-13 -0.04714861959003258 2110 14-15 -0.007130969500934725 2110 16-17 -0.17402960300537984 2110 18-19 0.5362767510728617 2110 20-21 0.007942644241467178 2110 22-23 -0.06315736330069655 2110 24-25 -0.09572246934776985 2110 26-27 -0.1156892335493751 2110 28-29 -0.10616714507784053 2110 30-31 0.02300371070740681 2110 32-33 -0.3120864434214212 2110 34-35 -0.21728736567938967 2110 36-37 -0.14478948460389773 2110 38-39 -0.12108017672455418 2110 40-41 0.24031431005472115 2110 42-43 -0.10356973617476228 2110 44-45 -0.29321332078525586 2110 46-47 -0.2973450344925581 2110 48-49 -0.24006856084003658 2110 50-51 -0.36430677235048137 2110 52-53 0.30420352359391956 2110 54-55 -0.1617268221185455 2110 56-57 -0.1737956032434269 2110 58-59 -0.27803800807535595 2110 60-61 -0.4862939384817153 2110 62-63 -0.2719507505388421 2110 64-65 0.39111258709566954 2110 66-67 0.01870670962992449 2110 68-69 -0.3697583350605882 2110 70-71 -0.2669656759215542 2110 72-73 -0.27177761824208346 2110 74-75 -0.06399479640527517 2110 76 -0.7559451032266011 2109 1 -0.058394129058683575 2109 2 0.057525904007242445 2109 3 0.030278446640259915 2109 4 -0.03373714485547907 2109 5 -0.09378291761104407 2109 6 0.1170104761705133 2109 7 -0.049969399104917045 2109 8 -0.09735242429631796 2109 9 0.008260783613359024 2109 10-11 -0.012081291120829007 2109 12-13 -0.007940653675319709 2109 14-15 -0.0165425982018661 2109 16-17 -0.028777254993400447 2109 18-19 0.41793901515119813 2109 20-21 0.11215420033801138 2109 22-23 -0.10945049609606627 2109 24-25 -0.0886196181997363 2109 26-27 -0.10409145010290644 2109 28-29 -0.06737426506538213 2109 30-31 0.24560519643873135 2109 32-33 0.11354184326288674 2109 34-35 0.1489644614652761 2109 36-37 -0.045250076291928565 2109 38-39 -0.25722443003302686 2109 40-41 0.1258424100962614 2109 42-43 0.012584195339364612 2109 44-45 -0.035785158542424256 2109 46-47 -0.2177681653586987 2109 48-49 -0.09599528825823711 2109 50-51 -0.18981801868545034 2109 52-53 0.19640551378234505 2109 54-55 -0.15350764146064222 2109 56-57 -0.033764993089519635 2109 58-59 -0.16527796465148015 2109 60-61 -0.2637850398588597 2109 62-63 -0.0360740694352657 2109 64-65 0.4931977518691184 2109 66-67 -0.07878772630953534 2109 68-69 -0.20852963734916585 2109 70-71 -0.21898525978075156 2109 72-73 -0.186425158149774 2109 74-75 -0.10650260447129511 2109 76 -0.4764884222740946 2108 1 -0.22520898929211342 2108 2 -0.020118638727311122 2108 3 -0.0874457939521136 2108 4 -0.05253649166346008 2108 5 0.019580252734755277 2108 6 -0.020744831063915115 2108 7 0.003186811972931025 2108 8 0.04428773983625689 2108 9 -0.059567329271494884 2108 10-11 -0.0448612342293373 2108 12-13 -0.06069366750172378 2108 14-15 -0.07986027630180104 2108 16-17 -0.17982342890887537 2108 18-19 0.4875662328468877 2108 20-21 0.0029454620578093227 2108 22-23 -0.1552997296092613 2108 24-25 -0.17367089542823066 2108 26-27 -0.14467228002212096 2108 28-29 -0.08734846845204913 2108 30-31 0.2052438908596983 2108 32-33 0.16391400374845944 2108 34-35 0.01683341280473627 2108 36-37 -0.04794891407856028 2108 38-39 -0.034797207530886 2108 40-41 0.32615878910719687 2108 42-43 0.03119775163795424 2108 44-45 -0.1336296012278595 2108 46-47 -0.12466413846514257 2108 48-49 -0.0775346914901931 2108 50-51 -0.07342331782869138 2108 52-53 0.2994908477014704 2108 54-55 -0.2206162634436737 2108 56-57 -0.24688678351250815 2108 58-59 -0.11024677637064428 2108 60-61 -0.2820678404608614 2108 62-63 -0.26978300120483567 2108 64-65 0.28064170487392914 2108 66-67 -0.12455530917829805 2108 68-69 -0.10740693934659618 2108 70-71 -0.2650156053888537 2108 72-73 -0.31845490682501776 2108 74-75 0.005315589255580733 2108 76 -0.8564048326320446 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 3.0 12 56.0 13 388.0 14 1172.0 15 1910.0 16 2601.0 17 3820.0 18 5169.0 19 7211.0 20 8326.0 21 7849.0 22 7436.0 23 7045.0 24 7080.0 25 7483.0 26 7906.0 27 8768.0 28 10321.0 29 12032.0 30 15223.0 31 21840.0 32 32691.0 33 44188.0 34 69047.0 35 122442.0 36 300237.0 37 1332205.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.49213357731105 2.981243357012085 49.27019456097951 5.256428504697354 2 3.809583902557608 2.5744833938141767 4.92323359496862 88.6926991086596 3 4.105458243272393 46.19406011106171 5.193624296815426 44.506857348850474 4 4.5248866565025585 2.0682345218687286 88.49851475874428 4.908364062884425 5 3.7590568412320384 2.6824342402280514 49.046564624502736 44.51194429403717 6 47.733790375793184 2.4354728340007505 6.14806238746968 43.68267440273639 7 3.9480075071571847 2.962118399627479 49.93003004721566 43.15984404599968 8 5.061755025437171 3.0686018580067294 88.5941884586018 3.275454657954295 9 5.936758510483753 3.059748616864495 87.59631568212266 3.407177190529086 10-11 68.28959783296135 23.482561805161197 3.3538131790032426 4.874027182874212 12-13 23.4356543009877 25.813091938219053 23.878976682715 26.87227707807825 14-15 4.225784062111601 45.073293586682766 26.343870646809968 24.35705170439566 16-17 25.68286125014613 3.704323267540545 45.75457739469167 24.858238087621654 18-19 24.0524708613421 44.97233728990061 4.271957872267785 26.70323397648951 20-21 26.389873261695453 2.3580681151743086 44.71610688258792 26.53595174054232 22-23 23.358322951562986 3.167772832680101 47.645991658388155 25.82791255736876 24-25 4.715231341060599 25.842537524780518 23.391437008211014 46.05079412594787 26-27 13.878653857347384 35.659534671689045 24.948384625882085 25.51342684508149 28-29 25.96579811968897 3.7897007946884465 23.83725394959718 46.407247136025404 30-31 46.98608280275028 5.423099328963452 25.346731564348147 22.244086303938126 32-33 59.25679730822338 11.914237038928336 4.88630432943057 23.942661323417703 34-35 4.403386927235651 44.67198741568021 26.647033014763387 24.27759264232074 36-37 20.058827586308094 15.132781497606446 35.54798872459034 29.260402191495118 38-39 46.360706478860564 3.9738824495010636 46.51165179468894 3.1537592769494376 40-41 24.297695858395098 43.210542302595954 27.7522941389098 4.739467700099147 42-43 37.21689804930326 3.1797565016295346 54.87207066549471 4.731274783572493 44-45 4.00660520267319 5.123898908703518 85.89091730828208 4.978578580341207 46-47 5.845438061795623 84.39200488738041 4.996333975560163 4.7662230752638 48-49 18.739988133722093 24.237655231311713 33.54304753994842 23.47930909501778 50-51 47.180413891469044 22.424795140402132 24.86657774295177 5.528213225177052 52-53 44.168233103393625 25.39950862066014 25.706608479839797 4.725649796106433 54-55 6.00183716981935 63.37282074534508 3.863950629240446 26.761391455595124 56-57 22.388966415890053 26.120632013809097 5.471303025900866 46.01909854439998 58-59 2.808482872402295 26.620668943074637 44.195624346706616 26.375223837816446 60-61 4.493019879683964 44.46677319903798 27.719620298672158 23.320586622605894 62-63 3.5391198313090717 22.8766528291975 60.29394717109597 13.29028016839745 64-65 25.194636794559315 46.673260130235576 23.890764699926486 4.2413383752786205 66-67 23.270842168232125 24.11346529064799 25.906344447819436 26.709348093300445 68-69 4.16721082306284 26.367079834224285 45.09708972931093 24.368619613401947 70-71 43.14169734730483 5.868378228070252 25.328095736308416 25.661828688316508 72-73 26.046773482733 65.91913028889446 3.710217276146287 4.323878952226248 74-75 24.05036760515914 28.31992874363704 22.888832149884884 24.740871501318935 76 14.943537354074374 75.43519060636876 3.4783455102083742 6.142926529348494 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 0.5 7 0.5 8 1.0 9 1.0 10 1.5 11 1.5 12 1.0 13 1.0 14 0.5 15 1.5 16 3.0 17 3.0 18 3.5 19 4.5 20 8.5 21 12.0 22 15.5 23 33.5 24 74.0 25 100.0 26 139.5 27 286.5 28 693.5 29 993.0 30 1754.5 31 3932.0 32 19658.5 33 33969.0 34 126727.0 35 150904.5 36 296478.0 37 510632.0 38 294289.5 39 50072.0 40 18553.0 41 30498.5 42 46088.0 43 29246.0 44 12051.0 45 14345.5 46 16993.0 47 38697.0 48 241689.0 49 255263.0 50 87549.0 51 149829.5 52 121956.0 53 37487.0 54 43172.0 55 27036.5 56 8193.0 57 5030.5 58 4576.0 59 4687.0 60 5155.5 61 5927.5 62 6342.0 63 6897.5 64 7949.0 65 8798.5 66 9450.0 67 9748.0 68 9726.0 69 9451.5 70 8792.0 71 8385.0 72 7891.5 73 6749.5 74 5384.0 75 4667.0 76 4122.0 77 2996.5 78 2006.0 79 1596.0 80 1279.0 81 780.5 82 470.0 83 341.0 84 280.5 85 179.5 86 113.0 87 87.0 88 71.0 89 47.0 90 37.0 91 29.5 92 24.0 93 29.0 94 28.0 95 19.0 96 16.0 97 18.0 98 21.0 99 56.0 100 90.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 2044449.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 11.223883762540122 #Duplication Level Percentage of deduplicated Percentage of total 1 92.37912169005853 10.368525239347658 2 3.385608913510288 0.7599936182131845 3 1.6462226286789001 0.5543103429466567 4 0.7249104094705439 0.32545240696610994 5 0.34633517437945865 0.19436128700570537 6 0.22156999215691264 0.14921255023416682 7 0.16421980985986998 0.12902288401615344 8 0.12967144121538565 0.11643337468180345 9 0.06761140314544958 0.06829762769340891 >10 0.6116507700290799 1.4291233519653885 >50 0.13052598678614777 1.0357759057670053 >100 0.13952415117077832 3.487937075343707 >500 0.02718223167543228 2.221956923180342 >1k 0.019161243243444953 4.795967556880042 >5k 0.0031192721559095806 2.4481736131828735 >10k+ 0.003564882463896664 71.9154562425758 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TCATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTG 479514 23.454436867830893 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAA 434261 21.24097984346883 No Hit TCATTGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTG 217251 10.626383930340156 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAA 209144 10.22984677045013 No Hit TCATTGTTTGGCATAGTCACGTTCAGCACTGGATATGTGCTTCCTGATTT 38696 1.8927349129276398 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCAC 35238 1.7235939854699238 No Hit TCATTGTTTGGCATAGTCACGTTCAATGCTAGGTATGTGTAGTTTAAGTG 12608 0.6166942780181849 No Hit ATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGG 11172 0.5464553040941593 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCATCCAGCACACTGGAGTTTAACAA 8375 0.4096458263326696 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGTAGTTTAACAA 7978 0.3902273913411389 No Hit TGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTCAACC 7906 0.3867056600580401 No Hit GCCTCCCTTAGGTCACTAATTGCCTCATCCGGCACACTGGAGTTTAACAA 7886 0.3857274013682904 No Hit CATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGG 6012 0.2940645621387474 No Hit TCATTGTTTGGCATAGTCACGTTCAGTGCTAGATATTTGTATTTTAATTG 5774 0.28242328373072645 No Hit TCATTGTTTGGCATAGTCACTCAAAACGGAACAAGTTCTGCTTGCATAAG 5018 0.24544510525818936 No Hit CTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAATG 4920 0.24065163767841602 No Hit TGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTC 4706 0.2301842696980947 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCATCCAGCACCCTGGAGTTTAACAA 4053 0.19824412347776835 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGTAGTTTAACAA 3773 0.18454850182127314 No Hit CTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAATG 3681 0.18004851184842469 No Hit TTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGTCAAC 3581 0.17515721839967638 No Hit CCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAAT 3368 0.16473876335384252 No Hit TCATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTATTTTAAGTG 3328 0.1627822459743432 No Hit TCATTGTTTGGCATAGTCGCGTTCAGTGCTGGATATTTGTATTTTAATTG 3274 0.16014094751201913 No Hit TTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTGGGT 3269 0.1598963828395817 No Hit TCATTGTTTGGCATAGTCACGTTCAGTGCTGGGTATGTGTAGTTTAAGTG 3192 0.15613008688404553 No Hit CATTGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTGG 2674 0.13079318681952937 No Hit TCATTGTTTGGCATAGTCACGTTCAGTGCTGGGTATTTGTATTTTAATTG 2375 0.11616821940777196 No Hit TCATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGGAGTTTAAGTG 2362 0.1155323512594347 No Hit TCATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAATTG 2314 0.11318453040403552 No Hit ATTGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTGGT 2245 0.1098095379243992 No Hit TCATTGTTTGGACTGGAGTCACTCAAAACGGAACAAGTTCTGCTTGCATA 2241 0.10961388618644925 No Hit TCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAATGA 2174 0.1063367195757879 No Hit CCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAAT 2123 0.10384215991692627 No Hit TCATTGTTTGGCATAGTCACGTCCAATGCTGGGTATGTGTAGTTTAAGTG 2082 0.10183672960293946 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 9.782586897496587E-4 0.0 0.0 2 9.782586897496587E-4 0.0 0.0 3 9.782586897496587E-4 0.0 0.0 4 9.782586897496587E-4 0.0 0.0 5 9.782586897496587E-4 0.0 0.0 6 9.782586897496587E-4 0.0 0.0 7 9.782586897496587E-4 0.0 0.0 8 9.782586897496587E-4 0.0 0.0 9 9.782586897496587E-4 0.0 0.0 10 9.782586897496587E-4 4.8912934487482935E-5 0.0 11 9.782586897496587E-4 4.8912934487482935E-5 0.0 12 9.782586897496587E-4 4.8912934487482935E-5 0.0 13 9.782586897496587E-4 4.8912934487482935E-5 0.0 14 9.782586897496587E-4 4.8912934487482935E-5 0.0 15 9.782586897496587E-4 4.8912934487482935E-5 0.0 16 9.782586897496587E-4 4.8912934487482935E-5 0.0 17 9.782586897496587E-4 4.8912934487482935E-5 0.0 18 9.782586897496587E-4 4.8912934487482935E-5 0.0 19 9.782586897496587E-4 4.8912934487482935E-5 0.0 20 9.782586897496587E-4 4.8912934487482935E-5 0.0 21 9.782586897496587E-4 4.8912934487482935E-5 0.0 22 0.0010271716242371417 4.8912934487482935E-5 0.0 23 0.0010271716242371417 4.8912934487482935E-5 0.0 24 0.0010271716242371417 4.8912934487482935E-5 0.0 25 0.0010271716242371417 4.8912934487482935E-5 0.0 26 0.0010271716242371417 4.8912934487482935E-5 0.0 27 0.0010271716242371417 4.8912934487482935E-5 0.0 28 0.0010271716242371417 4.8912934487482935E-5 0.0 29 0.0010271716242371417 4.8912934487482935E-5 0.0 30 0.0010271716242371417 4.8912934487482935E-5 0.0 31 0.0011739104276995904 4.8912934487482935E-5 0.0 32 0.0012717362966745563 4.8912934487482935E-5 0.0 33 0.0013206492311620393 4.8912934487482935E-5 0.0 34 0.0014184751001370052 4.8912934487482935E-5 0.0 35 0.001516300969111971 4.8912934487482935E-5 0.0 36 0.0016141268380869369 4.8912934487482935E-5 0.0 37 0.0017119527070619028 4.8912934487482935E-5 0.0 38 0.0019076044450118345 4.8912934487482935E-5 0.0 39 0.0021032561829617664 4.8912934487482935E-5 0.0 40 0.0023967337898866637 4.8912934487482935E-5 0.0 41 0.0024945596588616297 4.8912934487482935E-5 0.0 42 0.0027391243312990445 4.8912934487482935E-5 0.0 43 0.0032282536761738738 4.8912934487482935E-5 0.0 44 0.0033260795451488397 4.8912934487482935E-5 0.0 45 0.0034728183486112886 4.8912934487482935E-5 0.0 46 0.003864121824511152 4.8912934487482935E-5 0.0 47 0.004255425300411016 4.8912934487482935E-5 0.0 48 0.004548902907335913 4.8912934487482935E-5 0.0 49 0.004940206383235776 4.8912934487482935E-5 0.0 50 0.005380422793623123 4.8912934487482935E-5 0.0 51 0.005771726269522986 4.8912934487482935E-5 0.0 52 0.006211942679910333 4.8912934487482935E-5 0.0 53 0.0066032461558101965 4.8912934487482935E-5 0.0 54 0.007483678976584889 4.8912934487482935E-5 0.0 55 0.007874982452484752 4.8912934487482935E-5 0.0 56 0.008510850600822031 4.8912934487482935E-5 0.0 57 0.009097805814671825 4.8912934487482935E-5 0.0 58 0.010467367980321348 4.8912934487482935E-5 0.0 59 0.011347800801096042 4.8912934487482935E-5 0.0 60 0.0123260594908457 4.8912934487482935E-5 0.0 61 0.013157579377132909 4.8912934487482935E-5 0.0 62 0.014135838066882568 4.8912934487482935E-5 0.0 63 0.015016270887657262 4.8912934487482935E-5 0.0 64 0.015896703708431954 4.8912934487482935E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGTCAAG 15 0.002225153 70.0 21 GTTCTAA 15 0.002225153 70.0 13 GTACACT 50 0.0 70.0 32 ATATGTC 20 7.930092E-5 70.0 33 CGCCTTA 15 0.002225153 70.0 21 TAGTTAT 20 7.930092E-5 70.0 30 CCCGTAG 40 1.3460522E-10 70.0 5 TATCTAG 15 0.002225153 70.0 53 CACAGTA 15 0.002225153 70.0 46 GAGTTAA 60 0.0 70.0 40 TAGGCTA 15 0.002225153 70.0 9 ATAGTCT 70 0.0 70.0 13 AGCTAAG 15 0.002225153 70.0 65 GCTAAGA 15 0.002225153 70.0 66 AAGTGTA 15 0.002225153 70.0 35 GTAACAC 15 0.002225153 70.0 42 AGGCTAC 15 0.002225153 70.0 10 TCATTGT 88755 0.0 69.84621 1 GCTAGGT 1550 0.0 69.77419 28 TAGGTAT 1530 0.0 69.77124 30 >>END_MODULE