##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AHV5L l01n01 kokuya8.34100000033d4b.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2824333 Sequences flagged as poor quality 0 Sequence length 76 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.690727686855624 34.0 34.0 34.0 34.0 34.0 2 33.856212422543656 34.0 34.0 34.0 34.0 34.0 3 33.84452045845869 34.0 34.0 34.0 34.0 34.0 4 33.89117890843608 34.0 34.0 34.0 34.0 34.0 5 33.91299680313901 34.0 34.0 34.0 34.0 34.0 6 37.74980251974537 38.0 38.0 38.0 38.0 38.0 7 37.768361237856865 38.0 38.0 38.0 38.0 38.0 8 37.79445518641038 38.0 38.0 38.0 38.0 38.0 9 37.770356045126405 38.0 38.0 38.0 38.0 38.0 10-11 37.54585808401488 38.0 38.0 38.0 37.0 38.0 12-13 37.57799186569006 38.0 38.0 38.0 37.0 38.0 14-15 37.5922771854452 38.0 38.0 38.0 37.0 38.0 16-17 37.75560229618816 38.0 38.0 38.0 38.0 38.0 18-19 37.68917475382683 38.0 38.0 38.0 38.0 38.0 20-21 37.62447469898203 38.0 38.0 38.0 37.0 38.0 22-23 37.650507925234024 38.0 38.0 38.0 37.0 38.0 24-25 37.71688997012746 38.0 38.0 38.0 38.0 38.0 26-27 37.29972634246741 38.0 38.0 38.0 37.0 38.0 28-29 37.52361885089329 38.0 38.0 38.0 37.5 38.0 30-31 37.53320182145661 38.0 38.0 38.0 37.0 38.0 32-33 37.474393954253976 38.0 38.0 38.0 37.5 38.0 34-35 37.529309752072436 38.0 38.0 38.0 37.0 38.0 36-37 37.141420292862065 38.0 38.0 38.0 36.5 38.0 38-39 37.65662299735902 38.0 38.0 38.0 37.5 38.0 40-41 37.25453549563738 38.0 38.0 38.0 37.0 38.0 42-43 37.363765002214684 38.0 38.0 38.0 37.0 38.0 44-45 37.49442912716029 38.0 38.0 38.0 37.0 38.0 46-47 37.354234610437224 38.0 38.0 38.0 37.0 38.0 48-49 37.537336071915036 38.0 38.0 38.0 37.0 38.0 50-51 37.111623523146875 38.0 38.0 38.0 36.5 38.0 52-53 36.9154945610167 38.0 38.0 38.0 36.5 38.0 54-55 37.17389981988668 38.0 38.0 38.0 37.0 38.0 56-57 37.38339140604171 38.0 38.0 38.0 37.0 38.0 58-59 37.393978861557756 38.0 38.0 38.0 37.0 38.0 60-61 37.339120599447725 38.0 38.0 38.0 37.0 38.0 62-63 37.171637692864124 38.0 38.0 38.0 37.0 38.0 64-65 37.30343235022216 38.0 38.0 38.0 37.0 38.0 66-67 37.44915808440435 38.0 38.0 38.0 37.0 38.0 68-69 37.52858710357454 38.0 38.0 38.0 37.0 38.0 70-71 37.484811104073074 38.0 38.0 38.0 37.0 38.0 72-73 37.10464116660465 38.0 38.0 38.0 37.0 38.0 74-75 37.249846069850825 38.0 38.0 38.0 37.0 38.0 76 35.813565539191025 38.0 36.0 38.0 31.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.03501583993217139 1101 2 -0.005517568351478985 1101 3 0.03648267169756991 1101 4 0.021066633002533308 1101 5 0.03351858866377455 1101 6 -0.030049529767225636 1101 7 -0.06340836503670033 1101 8 -0.08221021200748169 1101 9 -0.43588625626154 1101 10-11 -0.5077091538931455 1101 12-13 -0.7114799261485771 1101 14-15 -0.24667863168760107 1101 16-17 -0.05223742069549786 1101 18-19 0.026786421932150972 1101 20-21 0.1384391736113244 1101 22-23 0.09530492525709633 1101 24-25 0.0457314707593639 1101 26-27 0.09633328146198039 1101 28-29 0.0895435895680734 1101 30-31 0.13545806267893568 1101 32-33 0.12214472323088188 1101 34-35 0.13966658695908762 1101 36-37 0.23050265848637963 1101 38-39 0.056155976875011504 1101 40-41 0.17165414783800514 1101 42-43 0.14366250770475375 1101 44-45 0.1268903187740591 1101 46-47 0.10240880596698787 1101 48-49 0.08616496688214426 1101 50-51 0.20973111902456054 1101 52-53 0.24289152492424648 1101 54-55 0.18977140281609906 1101 56-57 0.11445014245487783 1101 58-59 0.1564952024003432 1101 60-61 0.13041911645742488 1101 62-63 0.12417699904327861 1101 64-65 0.1328940806053538 1101 66-67 0.1834063295206434 1101 68-69 0.09045810599845794 1101 70-71 0.10091872892810017 1101 72-73 0.11001869800980302 1101 74-75 0.16266332382993198 1101 76 0.3980208862310448 1102 1 0.06493039631916986 1102 2 0.015772772520790568 1102 3 0.048919796926767845 1102 4 0.019602731713938226 1102 5 0.02889431088578931 1102 6 0.01082097727169895 1102 7 0.020487720129267473 1102 8 -0.0029188679343690183 1102 9 -0.20403889431760547 1102 10-11 -0.19286745186984433 1102 12-13 0.030517279787773077 1102 14-15 0.12009697979476641 1102 16-17 0.05291296968876935 1102 18-19 0.07893191925037968 1102 20-21 0.13358984672159835 1102 22-23 0.10367520357385729 1102 24-25 0.045099903732968016 1102 26-27 0.06688228455099932 1102 28-29 0.12234193142766259 1102 30-31 0.10600712533101841 1102 32-33 0.12768454487206782 1102 34-35 0.11824979370104671 1102 36-37 0.17064108384226273 1102 38-39 0.07624546680551703 1102 40-41 0.1338029322432206 1102 42-43 0.09787312397122605 1102 44-45 0.12789687499968494 1102 46-47 0.04065689820173901 1102 48-49 -5.286564391226989E-4 1102 50-51 0.1597703154918193 1102 52-53 0.17298898756947523 1102 54-55 0.16864545600162728 1102 56-57 0.10261450490454394 1102 58-59 0.13326016718347944 1102 60-61 0.14912662416725908 1102 62-63 0.10922792069830933 1102 64-65 0.1419531064903481 1102 66-67 0.18107509074231842 1102 68-69 0.04825719835265829 1102 70-71 0.09341249227816206 1102 72-73 0.1900098882354868 1102 74-75 0.20763560159140582 1102 76 0.5923441132212801 1103 1 0.007942522584215794 1103 2 0.011686301526168563 1103 3 -0.009758757992251788 1103 4 -0.00526676346209598 1103 5 0.014463891974983767 1103 6 0.041816468259391115 1103 7 0.028673441145798506 1103 8 0.03386333965926269 1103 9 0.08287181790441878 1103 10-11 0.1960285893938689 1103 12-13 0.19941365521115273 1103 14-15 0.1344767191470595 1103 16-17 0.07008788314062286 1103 18-19 0.06329191778357313 1103 20-21 0.14383400313435146 1103 22-23 0.08751997315767568 1103 24-25 0.04628680549986797 1103 26-27 0.05084944705451022 1103 28-29 0.031099021803477456 1103 30-31 0.06147576718552017 1103 32-33 0.06700660794997759 1103 34-35 0.13465911642213513 1103 36-37 -0.03718366140267193 1103 38-39 0.05624777734890074 1103 40-41 0.07970280118278339 1103 42-43 0.05868437154718009 1103 44-45 0.053849416549780926 1103 46-47 0.09057001272214649 1103 48-49 -0.03438123234179358 1103 50-51 0.14464072107433168 1103 52-53 -0.018712732789218478 1103 54-55 0.10859152850648712 1103 56-57 0.09164297578359992 1103 58-59 0.11787401990304858 1103 60-61 0.09415698348493606 1103 62-63 0.08751934966978325 1103 64-65 0.1699042918760938 1103 66-67 0.12789677600552096 1103 68-69 0.05853494932004821 1103 70-71 0.1487922524261407 1103 72-73 0.18728563376870255 1103 74-75 0.18081045884729008 1103 76 0.5379450640444077 2116 1 -0.015098453331987116 2116 2 0.04101248151893344 2116 3 -0.006199362878803072 2116 4 -0.0010697252309626037 2116 5 -0.0022212015404861063 2116 6 0.05543730934091684 2116 7 0.02698477348427275 2116 8 0.04697610140373598 2116 9 0.05034125150126556 2116 10-11 0.19672413194230387 2116 12-13 0.16481413074375695 2116 14-15 0.10184307361431166 2116 16-17 0.05424302158888139 2116 18-19 0.04917157578883291 2116 20-21 0.10936169570636167 2116 22-23 0.09347290628446814 2116 24-25 0.05220477415435454 2116 26-27 0.08479965385960497 2116 28-29 0.08346067568889026 2116 30-31 0.13152983846998012 2116 32-33 0.016133103534080817 2116 34-35 0.12653067529094386 2116 36-37 0.18852669914040376 2116 38-39 0.10158441205413737 2116 40-41 0.08622946323958303 2116 42-43 0.05991901380205178 2116 44-45 0.054129526738549316 2116 46-47 0.09521303038999207 2116 48-49 0.051351634041317595 2116 50-51 0.19666215664259568 2116 52-53 0.13911663885050274 2116 54-55 0.16233066033371557 2116 56-57 0.11881155399918697 2116 58-59 0.12541338954390113 2116 60-61 0.13131230635945457 2116 62-63 0.10637677618680641 2116 64-65 0.16453374498520645 2116 66-67 0.1906915483968632 2116 68-69 -0.026780985944277802 2116 70-71 0.081670542774269 2116 72-73 0.20583709650237836 2116 74-75 0.13814467203109615 2116 76 0.5825839080625528 1117 1 0.03002635266553 1117 2 -0.04221112497982915 1117 3 -0.044559533172964905 1117 4 -0.008833312617383626 1117 5 0.03148332301243073 1117 6 0.044205620893237096 1117 7 0.06948234305853163 1117 8 0.046883223786757355 1117 9 0.08437844464153699 1117 10-11 0.18737275705797174 1117 12-13 0.17867394241561385 1117 14-15 0.11783957299721948 1117 16-17 0.05527593774741746 1117 18-19 0.00961644124694061 1117 20-21 0.14932446307388858 1117 22-23 0.05769518896680381 1117 24-25 -0.03971882238858626 1117 26-27 0.07415686034681812 1117 28-29 0.09906946570696817 1117 30-31 -0.013689340882073964 1117 32-33 0.06879568784224688 1117 34-35 0.15712034115144746 1117 36-37 0.1556255571063403 1117 38-39 0.06402259907359564 1117 40-41 0.1005663738096132 1117 42-43 -0.21072284443134492 1117 44-45 -0.04238486083791315 1117 46-47 -0.12072852280754631 1117 48-49 -0.019311471942273783 1117 50-51 -0.14575926038014586 1117 52-53 -0.01977245853513665 1117 54-55 -0.04004875070277336 1117 56-57 0.035070853880490915 1117 58-59 0.05321617707846116 1117 60-61 0.11768823593374833 1117 62-63 0.10345754577866018 1117 64-65 0.1422235186097538 1117 66-67 0.12482234041886642 1117 68-69 0.14205115990348816 1117 70-71 0.12850323867510127 1117 72-73 0.06156608470035252 1117 74-75 0.17818878860356335 1117 76 0.5585838234026212 2117 1 -0.035352010512482934 2117 2 -8.684835099970201E-4 2117 3 -5.9133969060098934E-5 2117 4 0.031024284397460633 2117 5 0.024292434763729887 2117 6 0.026252029347759276 2117 7 0.04174583957480138 2117 8 -0.05848869994913741 2117 9 -0.17345112538339436 2117 10-11 -0.3221400659406797 2117 12-13 -0.7366471862181712 2117 14-15 -1.016888350196723 2117 16-17 -0.05534288611753624 2117 18-19 0.05198971957818799 2117 20-21 0.17355277509509648 2117 22-23 0.11814989896510752 2117 24-25 0.05362972605383476 2117 26-27 0.1620020872289416 2117 28-29 0.12558487663794438 2117 30-31 0.11406418028017384 2117 32-33 0.10618924952673581 2117 34-35 0.16794168724981517 2117 36-37 0.2850263836200071 2117 38-39 0.09853925401466768 2117 40-41 0.2093258532346738 2117 42-43 0.18472886968469737 2117 44-45 0.14948424653148606 2117 46-47 0.03441400321482746 2117 48-49 0.1302745448625373 2117 50-51 0.24306525385259192 2117 52-53 0.2539796994339909 2117 54-55 0.20880488332760905 2117 56-57 0.10030794660875841 2117 58-59 0.1384470260382571 2117 60-61 0.1726110441590336 2117 62-63 0.21138647947879718 2117 64-65 0.17037333821940592 2117 66-67 0.16174139348882477 2117 68-69 0.18323749962912927 2117 70-71 0.1884993607790193 2117 72-73 0.12813663409564668 2117 74-75 0.20076955809317099 2117 76 0.377031046215329 1116 1 0.07660911315196728 1116 2 0.005032578242236241 1116 3 0.024426079392021904 1116 4 -0.004752957872341312 1116 5 -0.017154715021618472 1116 6 -0.008109437743584635 1116 7 0.024517191289859852 1116 8 -0.05493448095394626 1116 9 -0.18463432461382467 1116 10-11 -0.25171325474028805 1116 12-13 -0.00958017671823086 1116 14-15 0.10656484121875565 1116 16-17 0.036109838715425724 1116 18-19 0.04149715348617633 1116 20-21 0.16142610170275162 1116 22-23 0.08825117091691226 1116 24-25 0.0704737603620913 1116 26-27 0.09704864703871863 1116 28-29 0.10819879138295363 1116 30-31 0.08875380051109971 1116 32-33 0.0673077791942518 1116 34-35 0.07078117227965208 1116 36-37 0.10472936368329755 1116 38-39 0.01527271736978264 1116 40-41 0.14870927572424364 1116 42-43 0.13369289524305117 1116 44-45 0.14540465721115936 1116 46-47 0.057699300117526775 1116 48-49 0.08698933106613538 1116 50-51 0.16110925408727184 1116 52-53 0.16768824188107345 1116 54-55 0.12338477554071403 1116 56-57 0.11143091355906165 1116 58-59 0.13578010530375906 1116 60-61 0.1537974696947515 1116 62-63 -0.01920438553609216 1116 64-65 0.13781598027947695 1116 66-67 0.14463891837545617 1116 68-69 0.1472289745057509 1116 70-71 0.08497203260797193 1116 72-73 0.08766520173779924 1116 74-75 0.18106555898835097 1116 76 0.400375922420082 2118 1 -0.13418361686952096 2118 2 0.020323065694043407 2118 3 -0.005265672109374009 2118 4 -0.0295309906008967 2118 5 0.007721628145233694 2118 6 0.06145546742123997 2118 7 0.07131776176479576 2118 8 -0.009798328150679936 2118 9 0.07791632285233163 2118 10-11 0.17075104103897587 2118 12-13 0.19301658688881673 2118 14-15 0.14893543476351567 2118 16-17 0.07050426297698209 2118 18-19 0.08083726309251205 2118 20-21 0.16338283417345423 2118 22-23 0.09474655882264216 2118 24-25 0.06043375785258576 2118 26-27 0.04585408407633906 2118 28-29 0.08753620489999747 2118 30-31 0.06647120852816357 2118 32-33 0.12001633466601902 2118 34-35 0.13687401970724267 2118 36-37 0.12451210506242205 2118 38-39 0.06960428684477904 2118 40-41 0.11080125286142817 2118 42-43 0.13904774682888643 2118 44-45 0.17198077531256928 2118 46-47 0.05088036581718569 2118 48-49 0.07482192477478833 2118 50-51 0.23043818284119055 2118 52-53 0.1980650011523437 2118 54-55 0.11050994960026372 2118 56-57 0.09640677174147783 2118 58-59 0.11256122203233332 2118 60-61 0.07534225124596361 2118 62-63 0.09192379553714858 2118 64-65 0.11508120143906098 2118 66-67 0.14539836455136168 2118 68-69 0.1461658752954449 2118 70-71 0.14620407956085302 2118 72-73 0.1481057967440833 2118 74-75 0.20325301563087095 2118 76 0.5751494404338331 1119 1 -0.04825252179561801 1119 2 -0.0038416033021704266 1119 3 -0.0031709800245423025 1119 4 -0.010598108550809116 1119 5 -0.016371657392532768 1119 6 0.05375383979359327 1119 7 0.07475231982294162 1119 8 0.052766864180334494 1119 9 0.08874600677701494 1119 10-11 0.21730083219610918 1119 12-13 0.21039937444061252 1119 14-15 0.11123100614454984 1119 16-17 0.02345260023961515 1119 18-19 0.07395842425624011 1119 20-21 0.13123023283612412 1119 22-23 0.053580071012909514 1119 24-25 0.04341205034329931 1119 26-27 0.06929807693089884 1119 28-29 0.05903314069180965 1119 30-31 0.1133316063807186 1119 32-33 0.1096332117044696 1119 34-35 0.16929957026758302 1119 36-37 0.09863520629904343 1119 38-39 0.02291497610140425 1119 40-41 0.10087397996316128 1119 42-43 0.16124215335675274 1119 44-45 0.14070467380837215 1119 46-47 0.05411658616944237 1119 48-49 0.06675268389932398 1119 50-51 0.17664272011564464 1119 52-53 0.20512175901895802 1119 54-55 0.11358733019427802 1119 56-57 0.10572181199719921 1119 58-59 0.11190054923784487 1119 60-61 0.17285827070389104 1119 62-63 0.17065912386598114 1119 64-65 0.1516213512040281 1119 66-67 0.13217594718522463 1119 68-69 0.08869462698761765 1119 70-71 0.09673943815442243 1119 72-73 0.17892781157226523 1119 74-75 0.20313167916910402 1119 76 0.24555547740845896 2119 1 6.599218197322898E-4 2119 2 -0.0024625522809884615 2119 3 0.003946829648327821 2119 4 -0.018739650029409916 2119 5 0.004950203057653368 2119 6 0.0690896352704371 2119 7 0.03989952574732314 2119 8 0.04883682704079462 2119 9 0.1000329413299923 2119 10-11 0.1904740298055927 2119 12-13 0.19057019457589774 2119 14-15 0.15730916262310757 2119 16-17 0.0064542510990932556 2119 18-19 0.06275307064180424 2119 20-21 0.13889884105935124 2119 22-23 0.08399030916818617 2119 24-25 -0.026874589569708007 2119 26-27 0.05486740070067242 2119 28-29 0.07341160588946138 2119 30-31 0.09613565605197749 2119 32-33 0.0597663661594936 2119 34-35 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-0.026688415127757992 2104 36-37 -0.04336262678428682 2104 38-39 -0.013894614866494237 2104 40-41 -0.0801663816265048 2104 42-43 0.02171629639466488 2104 44-45 0.008583786939247773 2104 46-47 -0.013126274571412466 2104 48-49 -0.029968594256011727 2104 50-51 -0.07403551727941249 2104 52-53 -0.09396574251990586 2104 54-55 -0.06880030196138165 2104 56-57 -0.04471350982949929 2104 58-59 0.0095916198891004 2104 60-61 -0.036427467761321 2104 62-63 -0.09634545415958229 2104 64-65 -0.19785651070209553 2104 66-67 -0.10874955581386558 2104 68-69 -0.042434879953844984 2104 70-71 -0.08105910166865726 2104 72-73 -0.13030936330886078 2104 74-75 -0.05406316698618241 2104 76 -0.29352215011362404 2111 1 0.06162561286733137 2111 2 -0.002136891344754588 2111 3 0.0010731582713034982 2111 4 0.019950178384917194 2111 5 -0.01429815840962334 2111 6 -0.11056719338117915 2111 7 -0.008584678060778117 2111 8 -0.05496718726210048 2111 9 -0.04491235908812996 2111 10-11 -0.3659847757224597 2111 12-13 -0.4204179981695546 2111 14-15 -0.21985938736748523 2111 16-17 -0.04565886289042709 2111 18-19 -0.024613248816848454 2111 20-21 0.1094928040583838 2111 22-23 0.003578241712183683 2111 24-25 0.02332792571099418 2111 26-27 -0.025829415513641152 2111 28-29 0.0017031951639197018 2111 30-31 -0.03169689858624025 2111 32-33 -0.017606744720694678 2111 34-35 -0.005608884385566171 2111 36-37 0.05708460355783984 2111 38-39 -6.796301563412044E-4 2111 40-41 0.001264147498552859 2111 42-43 0.09766303993760772 2111 44-45 0.07582229516775385 2111 46-47 0.03776218262320441 2111 48-49 0.0643812498310723 2111 50-51 -0.06201580280165331 2111 52-53 -0.06448273188754428 2111 54-55 -0.02044229448318191 2111 56-57 -0.06469116736436575 2111 58-59 -0.19720605776803524 2111 60-61 -0.04123981086829076 2111 62-63 -0.01562057639282699 2111 64-65 -0.08423871639264746 2111 66-67 -0.05123946969723647 2111 68-69 -0.08274635691560661 2111 70-71 -0.03665578503275668 2111 72-73 -0.058602345119389554 2111 74-75 0.029050032748486387 2111 76 -0.3322922797974641 2110 1 0.029630083711950306 2110 2 0.001504551273470156 2110 3 0.02166602276642493 2110 4 -0.0011468291403602393 2110 5 0.031711239915736655 2110 6 -0.08129899674612773 2110 7 -0.040701111054033845 2110 8 -0.041118293599247124 2110 9 -0.06727537977373999 2110 10-11 -0.34405882701903323 2110 12-13 -0.40373842140839145 2110 14-15 -0.10294801541056131 2110 16-17 -0.042913432498316695 2110 18-19 -0.05672197345730723 2110 20-21 -0.038309749836201945 2110 22-23 -0.004711672787941268 2110 24-25 -0.02044843821710174 2110 26-27 0.010936075866624151 2110 28-29 -0.05284986490551091 2110 30-31 -0.039001358831875166 2110 32-33 -0.044725860974807574 2110 34-35 0.011955686684395062 2110 36-37 -0.025067669086595856 2110 38-39 0.03082943490844059 2110 40-41 -0.15514302277770753 2110 42-43 -0.0451948988907418 2110 44-45 -0.12872391535992733 2110 46-47 -0.0050086183039823595 2110 48-49 -0.040852009532791556 2110 50-51 -0.007221772920892988 2110 52-53 -0.055433837889545146 2110 54-55 -0.011273837230397987 2110 56-57 -0.07375966739012796 2110 58-59 -0.04179468937203268 2110 60-61 -0.11284232295343344 2110 62-63 -0.24279930670144978 2110 64-65 -0.18132445631701444 2110 66-67 -0.21558004315679113 2110 68-69 -0.2278587375775203 2110 70-71 -0.18636221828156607 2110 72-73 -0.23234095068674776 2110 74-75 -0.145189071138752 2110 76 -0.39737390245600324 2109 1 -0.013143883934446876 2109 2 -0.00316170004635552 2109 3 0.02457526669590493 2109 4 -0.015267513246023157 2109 5 1.6024366823330638E-4 2109 6 -0.15433258068022582 2109 7 -0.09071242502182741 2109 8 -0.08985455515010443 2109 9 -0.17773762471228594 2109 10-11 -0.4940587351034935 2109 12-13 -0.47274420774252945 2109 14-15 -0.08504051119163591 2109 16-17 -0.0624059032783677 2109 18-19 -0.06476864449358288 2109 20-21 0.036524977660953084 2109 22-23 -0.026488219751598763 2109 24-25 -0.07304953946557902 2109 26-27 -0.09976289337576816 2109 28-29 -0.15877311977193642 2109 30-31 -0.04099981164259958 2109 32-33 -0.06534266774860953 2109 34-35 -0.10141035409869659 2109 36-37 -0.158349251183715 2109 38-39 -0.028238559865407353 2109 40-41 -0.2868462980126196 2109 42-43 -0.16649613386798734 2109 44-45 -0.23928823668731525 2109 46-47 -0.07684901314640769 2109 48-49 -0.20885645958204435 2109 50-51 -0.18151009593685075 2109 52-53 -0.4334458350404944 2109 54-55 -0.20783223518593275 2109 56-57 -0.03888706066197756 2109 58-59 -0.022915011163341603 2109 60-61 -0.15178531760194858 2109 62-63 -0.022773993160818407 2109 64-65 -0.09754553592691195 2109 66-67 -0.11873793534287103 2109 68-69 0.04955046072842606 2109 70-71 -0.04142185141488142 2109 72-73 -0.22978104431359014 2109 74-75 -0.25873137251009837 2109 76 -0.6223116254974244 2108 1 0.0066627058132979755 2108 2 0.019991005315610266 2108 3 0.030721857828197585 2108 4 0.018846585917842162 2108 5 -0.0015693556094191763 2108 6 0.02661837597032246 2108 7 0.029558636179103814 2108 8 0.05677198595275712 2108 9 0.03727476505751781 2108 10-11 0.1479334342175065 2108 12-13 0.1540897299253885 2108 14-15 0.08313636258507984 2108 16-17 0.005792326745059029 2108 18-19 0.01383209819701392 2108 20-21 0.0901275835370896 2108 22-23 -0.03702654622343715 2108 24-25 0.007346759270795644 2108 26-27 -0.04909395180095544 2108 28-29 -0.03279363899289223 2108 30-31 -0.0655909542076003 2108 32-33 -0.10221689825298341 2108 34-35 -0.014507620995964032 2108 36-37 -0.1316100709919894 2108 38-39 0.028385097854325636 2108 40-41 -0.08599297559672436 2108 42-43 0.018937605788856615 2108 44-45 -0.01275380816523608 2108 46-47 0.02600133777837499 2108 48-49 -0.029107961475205002 2108 50-51 0.01600733768323437 2108 52-53 -0.04062666804507842 2108 54-55 0.01390752963202857 2108 56-57 -0.006738862387237532 2108 58-59 -0.03124546276969653 2108 60-61 -0.09720853895534276 2108 62-63 -0.06626800595899596 2108 64-65 -0.06274980068408809 2108 66-67 -0.060973726524316874 2108 68-69 -0.14792233096611085 2108 70-71 -0.010713224816143452 2108 72-73 -0.02954218392729757 2108 74-75 -0.04344364532153122 2108 76 -0.12005569700664864 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 1.0 15 11.0 16 22.0 17 19.0 18 39.0 19 39.0 20 48.0 21 112.0 22 268.0 23 537.0 24 738.0 25 992.0 26 1409.0 27 2108.0 28 3035.0 29 4854.0 30 7680.0 31 12573.0 32 23523.0 33 40757.0 34 68109.0 35 112963.0 36 232556.0 37 2311940.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.73753696890558 1.2983596481009851 44.83593117383821 3.1281722091552235 2 1.9463710546879565 1.6602858090742134 2.7650422241286705 93.62830091210917 3 2.017821552911785 43.54359064600386 2.9334713718247816 51.50511642925958 4 2.0662223611734167 1.187147549527623 94.08826792024878 2.658362169050179 5 2.4137734466863505 1.241779917594703 44.77655432273744 51.56789231298151 6 44.51415608570236 1.4341439199981023 3.0999531570816896 50.95174683721785 7 2.2114955991379204 1.5256699546406178 45.586055185419 50.67677926080246 8 2.331311499033577 2.0968844679434047 93.82721513362624 1.744588899396778 9 3.3217754422017522 1.8701760734304347 92.97589908838654 1.8321493959812813 10-11 69.46526843683093 26.222580694273656 2.0660276249294967 2.2461232439659207 12-13 26.331402848035268 23.354806249829604 26.756016376255914 23.557774525879207 14-15 2.2388825963510675 47.61419775925856 23.242886019460173 26.904033624930207 16-17 23.22279277974658 2.0033048510922757 47.84743866959031 26.926463699570835 18-19 26.71984854477146 47.12539208372384 2.7842148925073635 23.370544478997342 20-21 23.769048983467055 1.3992519643150827 51.40250052180508 23.42919853041278 22-23 26.41066049931081 1.4674969275931697 44.50270205390087 27.619140519195152 24-25 2.695131912561302 23.004741296440613 26.36942952548442 47.93069726551366 26-27 23.64167044041903 25.9815503341851 23.382511906350985 26.994267319044884 28-29 23.388389400258397 1.763796974365275 26.51944724648262 48.328366378893705 30-31 48.79656541916268 2.0442525722002327 22.92815684269525 26.231025165941833 32-33 47.32882064544089 23.366826787067954 2.51726690868251 26.787085658808646 34-35 1.811507354125735 47.258715597629596 23.85076405650467 27.079012991739997 36-37 2.5067688548057188 1.8441168233349254 45.57134374735557 50.07777057450379 38-39 48.28867559172378 2.1110294005699752 48.080024558010685 1.5202704496955564 40-41 26.55281795737259 47.313755141479426 24.402257099286807 1.7311698018611827 42-43 26.25101218588601 1.8796119296131157 70.09107991161099 1.7782959728898824 44-45 1.797521751153281 3.0758943793100886 93.2699331134112 1.8566507561254286 46-47 2.3205655990281597 93.13384788550076 2.3322851802531783 2.213301335217908 48-49 2.8363156894034804 26.89272121948793 44.1518758588311 26.1190872322775 50-51 44.47850164977005 26.293553203535136 26.80710808534263 2.420837061352185 52-53 47.2202817443977 27.62048596960769 22.612276951761707 2.5469553342328966 54-55 2.589815011190253 72.07912452249788 1.6761302580113606 23.65493020830051 56-57 25.85058135850128 23.039599084102335 2.7655025097961183 48.34431704760026 58-59 1.4895021231561576 23.94062598142641 51.06164889196848 23.508223003448954 60-61 1.7971853885501463 47.769296325893585 24.033107993993628 26.400410291562643 62-63 2.0701701959365275 26.37897160143652 48.13364783826836 23.417210364358592 64-65 26.959922926935313 44.418540589937514 26.553614605643173 2.0679218774839936 66-67 26.18689085175155 26.546126111899692 23.359904798761335 23.907078237587424 68-69 2.6264077217523574 23.541292758325593 47.171349837289014 26.660949682633035 70-71 51.01305334746292 3.2790219850138067 22.974344739094153 22.73357992842912 72-73 26.990213264512363 68.70634588768392 2.1182700481848284 2.1851707996188834 74-75 23.456299239501856 23.566608470035224 26.115263320578702 26.861828969884215 76 49.3891832160018 45.45455511088813 2.3127230393866447 2.843538633723431 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 1.0 2 1.0 3 2.0 4 3.0 5 3.0 6 4.5 7 9.0 8 12.0 9 10.5 10 9.0 11 11.5 12 14.0 13 16.0 14 26.5 15 43.5 16 50.0 17 48.0 18 61.0 19 81.5 20 111.5 21 134.0 22 154.5 23 191.0 24 233.0 25 259.0 26 291.0 27 496.5 28 1843.5 29 3017.0 30 4922.5 31 7298.0 32 33949.5 33 60131.0 34 575979.5 35 584472.0 36 46093.0 37 15070.0 38 11434.5 39 6605.5 40 4904.0 41 5657.0 42 6918.0 43 5847.5 44 4981.5 45 5250.0 46 5314.0 47 6922.5 48 12946.0 49 47550.5 50 77740.0 51 659844.0 52 659239.0 53 45313.5 54 14097.0 55 10466.0 56 5718.0 57 3865.0 58 3129.0 59 2924.0 60 2822.0 61 3083.0 62 3241.0 63 3502.0 64 3976.5 65 4415.5 66 4766.0 67 4891.0 68 4974.0 69 5022.5 70 4910.5 71 4833.0 72 4704.0 73 4418.0 74 3978.0 75 3695.0 76 3436.5 77 2973.5 78 2527.0 79 2285.0 80 2072.5 81 1701.5 82 1394.0 83 1245.0 84 1087.0 85 844.5 86 649.5 87 539.0 88 452.5 89 301.5 90 195.5 91 133.5 92 113.0 93 82.0 94 40.5 95 21.5 96 13.0 97 9.0 98 2.5 99 1.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 8.851647450920271E-5 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 2824333.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 4.427145206702391 #Duplication Level Percentage of deduplicated Percentage of total 1 90.29822839937171 3.997633690319962 2 3.7402971816583404 0.33117677478842367 3 1.8311528995207347 0.24320339345557215 4 1.3975833269485498 0.24749217307469812 5 0.788916795771547 0.1746324605443507 6 0.4633036164668685 0.12306674309335078 7 0.23344337065436077 0.0723441389600232 8 0.15246876701608653 0.05400010968536729 9 0.10064239532722413 0.04010026482575708 >10 0.5544666868969403 0.525594431559267 >50 0.11471918135923813 0.35720750033338844 >100 0.22723903100646103 2.231161032398155 >500 0.04397220606648403 1.3291451289217708 >1k 0.044771700722116205 3.9017092069998878 >5k 0.00479696793451245 1.4460895128652451 >10k+ 0.003997473278760375 84.92544343817488 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAA 1265037 44.79064614547931 No Hit TCATTGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTG 1086755 38.47828850209943 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAA 20808 0.736740320634996 No Hit CTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAATG 14496 0.513253925794161 No Hit CATTGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTGG 12281 0.43482832937900734 No Hit CCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAAT 7667 0.27146232402482284 No Hit ATTGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTGGT 7375 0.26112359980214794 No Hit TGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTGGTGC 7270 0.25740590787276146 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAC 7112 0.25181166668377986 No Hit TTGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTGGTG 5944 0.21045676979308034 No Hit TCATTGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTT 5488 0.19431136484260178 No Hit TTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTGGTGCAAC 4575 0.16198514835184094 No Hit TCATTGTTTGGCATAGTCACGTCCAGTGCTGGATATTTGTATTTTAATTG 4239 0.1500885341778041 No Hit TCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAATGA 3859 0.1366340300524053 No Hit GTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTGGTGCA 3841 0.13599671143593903 No Hit TCATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTG 3337 0.11815179017488377 No Hit TCATTGTTTGGCATAGTCACGTTCAGTGCTGGGTATTTGTATTTTAATTG 3287 0.1163814606846997 No Hit TCATTGTTTGGCATAGTCACGTTCAGCGCTGGATATTTGTATTTTAATTG 2958 0.10473269263928864 No Hit TGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTGGTGCAACC 2932 0.10381212130439292 No Hit TCATTGTTTGGCATAGTCACGTTCAGCACTGGATATGTGCTTCCTGATTT 2923 0.1034934619961598 No Hit GCCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCAC 2918 0.10331642904714139 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 8.497581552883459E-4 0.0 0.0 2 8.497581552883459E-4 0.0 0.0 3 8.851647450920271E-4 0.0 0.0 4 8.851647450920271E-4 0.0 0.0 5 8.851647450920271E-4 0.0 0.0 6 8.851647450920271E-4 0.0 0.0 7 8.851647450920271E-4 0.0 0.0 8 8.851647450920271E-4 0.0 0.0 9 8.851647450920271E-4 0.0 0.0 10 9.205713348957081E-4 0.0 0.0 11 9.205713348957081E-4 0.0 0.0 12 9.559779246993892E-4 0.0 0.0 13 9.559779246993892E-4 0.0 0.0 14 9.559779246993892E-4 0.0 0.0 15 9.559779246993892E-4 0.0 0.0 16 9.559779246993892E-4 0.0 0.0 17 9.559779246993892E-4 0.0 0.0 18 9.559779246993892E-4 0.0 0.0 19 9.559779246993892E-4 0.0 0.0 20 0.0010621976941104325 0.0 0.0 21 0.0010621976941104325 0.0 0.0 22 0.0010621976941104325 0.0 0.0 23 0.0010621976941104325 0.0 0.0 24 0.0010621976941104325 0.0 0.0 25 0.0010621976941104325 0.0 0.0 26 0.0010621976941104325 0.0 0.0 27 0.0011330108737177946 0.0 0.0 28 0.0011330108737177946 0.0 0.0 29 0.0011684174635214757 0.0 0.0 30 0.0011684174635214757 0.0 0.0 31 0.0012746372329325189 0.0 0.0 32 0.0013100438227362 0.0 0.0 33 0.0013454504125398812 0.0 0.0 34 0.0014870767717546055 0.0 0.0 35 0.0015578899513619675 0.0 0.0 36 0.0016287031309693298 0.0 0.0 37 0.0016995163105766918 0.0 0.0 38 0.0018057360799877352 0.0 0.0 39 0.0019119558493987784 0.0 0.0 40 0.002088988798417184 0.0 0.0 41 0.002159801978024546 0.0 0.0 42 0.002266021747435589 0.0 0.0 43 0.0024784612862576755 0.0 0.0 44 0.0027263074148834434 0.0 0.0 45 0.00293874695370553 0.0 0.0 46 0.0031865930823312973 0.0 0.0 47 0.0033990326211533837 0.0 0.0 48 0.0036822853395828323 0.0 0.0 49 0.004036351237619643 0.0 0.0 50 0.00424879077644173 0.0 0.0 51 0.004425823725460135 0.0 0.0 52 0.00488610939290799 0.0 0.0 53 0.005310988470552162 0.0 0.0 54 0.005700460958392654 0.0 0.0 55 0.005842087317607378 0.0 0.0 56 0.006089933446233146 0.0 0.0 57 0.006373186164662595 0.0 0.0 58 0.006656438883092044 0.0 0.0 59 0.006904285011717811 0.0 0.0 60 0.007399977268969346 0.0 0.0 61 0.008037295885435605 0.0 0.0 62 0.008462174963079779 0.0 0.0 63 0.008993273810134995 0.0 0.0 64 0.009559779246993892 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATAGTA 130 0.0 70.00001 12 ACAATAA 55 0.0 70.00001 47 TATACTA 55 0.0 70.00001 66 TTAGGAC 15 0.002225241 70.0 8 AGTCAGT 30 1.0248368E-7 70.0 15 TCATTGA 15 0.002225241 70.0 1 TAGACAC 30 1.0248368E-7 70.0 14 TAGGTTA 40 1.3460522E-10 70.0 9 ATGTGTT 75 0.0 70.0 35 GTTTAAA 20 7.930614E-5 70.0 42 CAACCGA 145 0.0 70.0 54 CCTACTA 160 0.0 70.0 66 GTTAGGC 35 3.7161954E-9 70.0 6 TTCAAGT 15 0.002225241 70.0 58 CTCCAAT 200 0.0 70.0 57 ATCGTAT 15 0.002225241 70.0 42 GTAATAT 15 0.002225241 70.0 37 GTTACTA 40 1.3460522E-10 70.0 12 AGTGCAA 50 0.0 70.0 50 AATTGTG 20 7.930614E-5 70.0 46 >>END_MODULE