FastQCFastQC Report
Tue 25 Aug 2015
000000000-AHV5L l01n01 kokuya8.34100000033d4b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AHV5L l01n01 kokuya8.34100000033d4b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2824333
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAA126503744.79064614547931No Hit
TCATTGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTG108675538.47828850209943No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACACTGGAGTTTAACAA208080.736740320634996No Hit
CTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAATG144960.513253925794161No Hit
CATTGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTGG122810.43482832937900734No Hit
CCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAAT76670.27146232402482284No Hit
ATTGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTGGT73750.26112359980214794No Hit
TGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTGGTGC72700.25740590787276146No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAC71120.25181166668377986No Hit
TTGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTGGTG59440.21045676979308034No Hit
TCATTGTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTT54880.19431136484260178No Hit
TTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTGGTGCAAC45750.16198514835184094No Hit
TCATTGTTTGGCATAGTCACGTCCAGTGCTGGATATTTGTATTTTAATTG42390.1500885341778041No Hit
TCCCTTAGGTCACTAGTTGCCTCATCCGGCACCCTGGAGTTTAACAATGA38590.1366340300524053No Hit
GTTTGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTGGTGCA38410.13599671143593903No Hit
TCATTGTTTGGCATAGTCACGTTCAATGCTGGGTATGTGTAGTTTAAGTG33370.11815179017488377No Hit
TCATTGTTTGGCATAGTCACGTTCAGTGCTGGGTATTTGTATTTTAATTG32870.1163814606846997No Hit
TCATTGTTTGGCATAGTCACGTTCAGCGCTGGATATTTGTATTTTAATTG29580.10473269263928864No Hit
TGGCATAGTCACGTTCAGTGCTGGATATTTGTATTTTAATTGGTGCAACC29320.10381212130439292No Hit
TCATTGTTTGGCATAGTCACGTTCAGCACTGGATATGTGCTTCCTGATTT29230.1034934619961598No Hit
GCCTCCCTTAGGTCACTAGTTGCCTCGTCAGGCACTCTGGAGTTTATCAC29180.10331642904714139No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATAGTA1300.070.0000112
ACAATAA550.070.0000147
TATACTA550.070.0000166
TTAGGAC150.00222524170.08
AGTCAGT301.0248368E-770.015
TCATTGA150.00222524170.01
TAGACAC301.0248368E-770.014
TAGGTTA401.3460522E-1070.09
ATGTGTT750.070.035
GTTTAAA207.930614E-570.042
CAACCGA1450.070.054
CCTACTA1600.070.066
GTTAGGC353.7161954E-970.06
TTCAAGT150.00222524170.058
CTCCAAT2000.070.057
ATCGTAT150.00222524170.042
GTAATAT150.00222524170.037
GTTACTA401.3460522E-1070.012
AGTGCAA500.070.050
AATTGTG207.930614E-570.046
Produced by FastQC (version 0.11.2)